BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00040573
         (1278 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_022035444.1| piezo-type mechanosensitive ion channel homo...   706   0.0  
ref|XP_023749583.1| piezo-type mechanosensitive ion channel homo...   687   0.0  
gb|PLY61780.1| hypothetical protein LSAT_8X55460 [Lactuca sativa]     687   0.0  
ref|XP_021907032.1| piezo-type mechanosensitive ion channel homo...   587   0.0  
ref|XP_022010653.1| piezo-type mechanosensitive ion channel homo...   624   0.0  
gb|OTF93926.1| putative Piezo non-specific cation channel, R-Ras...   624   0.0  
ref|XP_023738449.1| piezo-type mechanosensitive ion channel homo...   622   0.0  
ref|XP_016484083.1| PREDICTED: piezo-type mechanosensitive ion c...   610   0.0  
gb|KVH94432.1| Piezo family [Cynara cardunculus var. scolymus]        617   0.0  
ref|XP_009770606.1| PREDICTED: piezo-type mechanosensitive ion c...   610   0.0  
dbj|GAU30701.1| hypothetical protein TSUD_39250 [Trifolium subte...   595   0.0  
ref|XP_009770604.1| PREDICTED: piezo-type mechanosensitive ion c...   610   0.0  
gb|OIT06222.1| piezo-type mechanosensitive ion channel-like prot...   607   0.0  
ref|XP_012847917.1| PREDICTED: LOW QUALITY PROTEIN: piezo-type m...   593   0.0  
ref|XP_018630180.1| PREDICTED: piezo-type mechanosensitive ion c...   608   0.0  
ref|XP_021834067.1| piezo-type mechanosensitive ion channel homo...   604   0.0  
ref|XP_009614902.1| PREDICTED: piezo-type mechanosensitive ion c...   608   0.0  
ref|XP_019228329.1| PREDICTED: piezo-type mechanosensitive ion c...   607   0.0  
ref|XP_020425451.1| piezo-type mechanosensitive ion channel homo...   602   0.0  
gb|ONH93697.1| hypothetical protein PRUPE_8G248000 [Prunus persica]   602   0.0  

>ref|XP_022035444.1| piezo-type mechanosensitive ion channel homolog [Helianthus annuus]
 gb|OTG29048.1| hypothetical protein HannXRQ_Chr04g0117981 [Helianthus annuus]
          Length = 2441

 Score =  706 bits (1821), Expect = 0.0
 Identities = 347/425 (81%), Positives = 376/425 (88%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSL AL LWSFYFASV AFGLL YVGY+I+A+PS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 331  VSLCALCLWSFYFASVCAFGLLAYVGYVIFAYPSLFRLHRLNGLLLVFILLWAVSTYIFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAFS+L+WDL   M+IWEMVGLWHYPIPGFF+LAQFC               FLYISDEE
Sbjct: 391  VAFSYLHWDLEKDMEIWEMVGLWHYPIPGFFILAQFCLGVLVALGNLVNNSVFLYISDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            D PTSNGDAT EV E+TKVLIVATIAW+LRKCSRAIMLVLIFLIAMKPGFIHA+YM+FFF
Sbjct: 451  DQPTSNGDATQEVTEDTKVLIVATIAWVLRKCSRAIMLVLIFLIAMKPGFIHALYMIFFF 510

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSHDI +KVR TLLLLCE+HFA LYSLQIT VSQALEKSGS+SLEILSQLGF   NS
Sbjct: 511  LYLLSHDIGDKVRHTLLLLCELHFALLYSLQITLVSQALEKSGSLSLEILSQLGFCGQNS 570

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
            PWDF+EIALLACFCAIHKHGY +LFSFSAIVQHTP  PVGFSILKAGLNKSVLLSVYASG
Sbjct: 571  PWDFVEIALLACFCAIHKHGYDLLFSFSAIVQHTPYPPVGFSILKAGLNKSVLLSVYASG 630

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            T+T++DDN SQERKVASYL ++GQKFLS+YRSFGTYIAF TIL+TVYM RPNFVSFGFIF
Sbjct: 631  TATNSDDNLSQERKVASYLGAIGQKFLSIYRSFGTYIAFLTILITVYMARPNFVSFGFIF 690

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQLVQKTKRRLWYPLKLY+I+MFII YILSIFP  +SWVSTK DLY  LGY 
Sbjct: 691  LLLFWITGRQLVQKTKRRLWYPLKLYSIVMFIITYILSIFPAFKSWVSTKVDLYAYLGYN 750

Query: 1262 PEGSL 1276
            P+ SL
Sbjct: 751  PDASL 755


>ref|XP_023749583.1| piezo-type mechanosensitive ion channel homolog [Lactuca sativa]
          Length = 2424

 Score =  687 bits (1773), Expect = 0.0
 Identities = 348/425 (81%), Positives = 370/425 (87%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALSLWSFYFASV AFGLLVYVGYII+AFPS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 331  VSLFALSLWSFYFASVCAFGLLVYVGYIIFAFPSLFQLHRLNGLLLIFILLWAVSTYIFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF++LN++LG  M+IWEMVGLWHYPIPGFF+LAQFC               FLYISDE 
Sbjct: 391  VAFAYLNFELGKDMEIWEMVGLWHYPIPGFFILAQFCLGVLVALGNLVNNSVFLYISDE- 449

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            DH  SNGDA  EV+EETKVLIVATIAW LRKCSRAIMLVLIFLIAMKPGFIHAIYM+FFF
Sbjct: 450  DHEASNGDANHEVKEETKVLIVATIAWGLRKCSRAIMLVLIFLIAMKPGFIHAIYMIFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSH+ISNKVR  LLLLCE+HFA LYSLQIT VSQALEKSGSI++E+LSQLG  EH S
Sbjct: 510  LYLLSHNISNKVRHVLLLLCELHFALLYSLQITLVSQALEKSGSITMEVLSQLGLREHGS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIALLACFCAI KHGYKMLFSFSAIVQHTP  PVGFSILKAGLNKSVLLSVYASG
Sbjct: 570  SWDFLEIALLACFCAIDKHGYKMLFSFSAIVQHTPYPPVGFSILKAGLNKSVLLSVYASG 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            T   NDD PSQERKVASYLS++GQKFLSLYRSFGTYIAF TILVTVYM  PNFVSFGFIF
Sbjct: 630  TRI-NDDTPSQERKVASYLSAIGQKFLSLYRSFGTYIAFITILVTVYMASPNFVSFGFIF 688

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQLVQKTKRRLW+PLK YAI MFI+ Y LSIFP+ E WVS   DLYTILGY 
Sbjct: 689  LLLFWITGRQLVQKTKRRLWFPLKAYAITMFILIYSLSIFPSFEIWVSENIDLYTILGYN 748

Query: 1262 PEGSL 1276
            P+ SL
Sbjct: 749  PQASL 753


>gb|PLY61780.1| hypothetical protein LSAT_8X55460 [Lactuca sativa]
          Length = 2433

 Score =  687 bits (1773), Expect = 0.0
 Identities = 348/425 (81%), Positives = 370/425 (87%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALSLWSFYFASV AFGLLVYVGYII+AFPS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 340  VSLFALSLWSFYFASVCAFGLLVYVGYIIFAFPSLFQLHRLNGLLLIFILLWAVSTYIFN 399

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF++LN++LG  M+IWEMVGLWHYPIPGFF+LAQFC               FLYISDE 
Sbjct: 400  VAFAYLNFELGKDMEIWEMVGLWHYPIPGFFILAQFCLGVLVALGNLVNNSVFLYISDE- 458

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            DH  SNGDA  EV+EETKVLIVATIAW LRKCSRAIMLVLIFLIAMKPGFIHAIYM+FFF
Sbjct: 459  DHEASNGDANHEVKEETKVLIVATIAWGLRKCSRAIMLVLIFLIAMKPGFIHAIYMIFFF 518

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSH+ISNKVR  LLLLCE+HFA LYSLQIT VSQALEKSGSI++E+LSQLG  EH S
Sbjct: 519  LYLLSHNISNKVRHVLLLLCELHFALLYSLQITLVSQALEKSGSITMEVLSQLGLREHGS 578

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIALLACFCAI KHGYKMLFSFSAIVQHTP  PVGFSILKAGLNKSVLLSVYASG
Sbjct: 579  SWDFLEIALLACFCAIDKHGYKMLFSFSAIVQHTPYPPVGFSILKAGLNKSVLLSVYASG 638

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            T   NDD PSQERKVASYLS++GQKFLSLYRSFGTYIAF TILVTVYM  PNFVSFGFIF
Sbjct: 639  TRI-NDDTPSQERKVASYLSAIGQKFLSLYRSFGTYIAFITILVTVYMASPNFVSFGFIF 697

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQLVQKTKRRLW+PLK YAI MFI+ Y LSIFP+ E WVS   DLYTILGY 
Sbjct: 698  LLLFWITGRQLVQKTKRRLWFPLKAYAITMFILIYSLSIFPSFEIWVSENIDLYTILGYN 757

Query: 1262 PEGSL 1276
            P+ SL
Sbjct: 758  PQASL 762


>ref|XP_021907032.1| piezo-type mechanosensitive ion channel homolog isoform X1 [Carica
            papaya]
          Length = 611

 Score =  587 bits (1513), Expect = 0.0
 Identities = 290/425 (68%), Positives = 340/425 (80%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSL ALS WSF+FAS+ AFGLL YVGYIIYAFPS+F LHRL+GLLL+FILLWAV TYIFN
Sbjct: 58   VSLLALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFWLHRLSGLLLVFILLWAVSTYIFN 117

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF+FLN  LG  MDIWEMVGLWHY IPGFFLLAQFC               F Y+SDE 
Sbjct: 118  VAFAFLNLKLGKDMDIWEMVGLWHYSIPGFFLLAQFCLGILVALGNLVNSSVFFYLSDEN 177

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
               ++N  ++ EV+EETKVLIVAT+AW LRKCSR IML LIFLIAMKPGFIHA+YM+FF 
Sbjct: 178  GQ-SANDTSSVEVDEETKVLIVATVAWGLRKCSRPIMLTLIFLIAMKPGFIHAVYMIFFL 236

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSH+IS K+RQ+L+LLCE HFA LY LQI  +S  L +  S+S +IL QLG  ++ S
Sbjct: 237  IYLLSHEISRKIRQSLILLCEAHFALLYILQIDLISNTLRREKSLSGQILLQLGLLKNES 296

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIALLACFCAIH HG++MLFSFSAIV+HTP  P GFSILKAGLNKSVLLSVYAS 
Sbjct: 297  SWDFLEIALLACFCAIHNHGFEMLFSFSAIVRHTPSPPFGFSILKAGLNKSVLLSVYASP 356

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            T   + +NPS ER++A++LS++GQKFLS+YRS GTYIAF TIL+ VY+ RPN +SFG+IF
Sbjct: 357  TLNYSHENPSYERRIAAFLSAIGQKFLSMYRSCGTYIAFLTILLAVYLVRPNCISFGYIF 416

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LL+FWITGRQLV+KTKRRLW+PLK YAI +FI  Y LS FP+ + W+ST  DLY  LGY 
Sbjct: 417  LLIFWITGRQLVEKTKRRLWFPLKAYAITVFIFIYSLSSFPSFKEWLSTIIDLYFYLGYN 476

Query: 1262 PEGSL 1276
             + SL
Sbjct: 477  SKASL 481


>ref|XP_022010653.1| piezo-type mechanosensitive ion channel homolog [Helianthus annuus]
          Length = 2480

 Score =  624 bits (1610), Expect = 0.0
 Identities = 305/425 (71%), Positives = 351/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALSLWSFYFASV A GLL YVGYII+AFPS+FHLHRLNG+LL+FILLWAV TY FN
Sbjct: 331  VSLFALSLWSFYFASVCALGLLTYVGYIIFAFPSLFHLHRLNGILLVFILLWAVSTYAFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            +AFS LN  LG   ++WEMVGLWHYP PGFF+LAQF                FLYISDE 
Sbjct: 391  IAFSHLNGILGKDTEVWEMVGLWHYPFPGFFILAQFSLGILVALVNLVNNSVFLYISDEV 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
             H TSNGD  P+ ++ETKVLIVATIAW+LRKC+RA+MLV IFLIAMKPGFIHA+YM+FFF
Sbjct: 451  PH-TSNGDPAPKAKKETKVLIVATIAWVLRKCNRALMLVQIFLIAMKPGFIHAVYMIFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSH I+ +V+  LLLLCEIHFA LY+LQI  V  AL K  +IS EILSQLG  E +S
Sbjct: 510  LYLLSHRINTRVQGALLLLCEIHFAILYTLQINMVCVALTKKETISWEILSQLGLLERDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDF+EIALLACFCAI KHG+K+LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVY   
Sbjct: 570  SWDFVEIALLACFCAIQKHGHKLLFSFSAIVQHTPCAPVGFSILKAGLNKSVLLSVYEWD 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
             +T N+DNPSQERKVASYLS++GQKFLS+YRS GTYIAF TIL+TVYMTRPN+VSFG++F
Sbjct: 630  NTTDNNDNPSQERKVASYLSAIGQKFLSIYRSLGTYIAFITILITVYMTRPNYVSFGYLF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQL + +++RLW+PLK Y+I+MFI+ YIL IF T E W+STK DLYT LGY 
Sbjct: 690  LLLFWITGRQLAETSRKRLWFPLKAYSIVMFILIYILCIFRTFEGWISTKVDLYTYLGYN 749

Query: 1262 PEGSL 1276
             + ++
Sbjct: 750  SKSTV 754


>gb|OTF93926.1| putative Piezo non-specific cation channel, R-Ras-binding domain
            protein [Helianthus annuus]
          Length = 2494

 Score =  624 bits (1610), Expect = 0.0
 Identities = 305/425 (71%), Positives = 351/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALSLWSFYFASV A GLL YVGYII+AFPS+FHLHRLNG+LL+FILLWAV TY FN
Sbjct: 345  VSLFALSLWSFYFASVCALGLLTYVGYIIFAFPSLFHLHRLNGILLVFILLWAVSTYAFN 404

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            +AFS LN  LG   ++WEMVGLWHYP PGFF+LAQF                FLYISDE 
Sbjct: 405  IAFSHLNGILGKDTEVWEMVGLWHYPFPGFFILAQFSLGILVALVNLVNNSVFLYISDEV 464

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
             H TSNGD  P+ ++ETKVLIVATIAW+LRKC+RA+MLV IFLIAMKPGFIHA+YM+FFF
Sbjct: 465  PH-TSNGDPAPKAKKETKVLIVATIAWVLRKCNRALMLVQIFLIAMKPGFIHAVYMIFFF 523

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSH I+ +V+  LLLLCEIHFA LY+LQI  V  AL K  +IS EILSQLG  E +S
Sbjct: 524  LYLLSHRINTRVQGALLLLCEIHFAILYTLQINMVCVALTKKETISWEILSQLGLLERDS 583

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDF+EIALLACFCAI KHG+K+LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVY   
Sbjct: 584  SWDFVEIALLACFCAIQKHGHKLLFSFSAIVQHTPCAPVGFSILKAGLNKSVLLSVYEWD 643

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
             +T N+DNPSQERKVASYLS++GQKFLS+YRS GTYIAF TIL+TVYMTRPN+VSFG++F
Sbjct: 644  NTTDNNDNPSQERKVASYLSAIGQKFLSIYRSLGTYIAFITILITVYMTRPNYVSFGYLF 703

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQL + +++RLW+PLK Y+I+MFI+ YIL IF T E W+STK DLYT LGY 
Sbjct: 704  LLLFWITGRQLAETSRKRLWFPLKAYSIVMFILIYILCIFRTFEGWISTKVDLYTYLGYN 763

Query: 1262 PEGSL 1276
             + ++
Sbjct: 764  SKSTV 768


>ref|XP_023738449.1| piezo-type mechanosensitive ion channel homolog [Lactuca sativa]
          Length = 2457

 Score =  622 bits (1605), Expect = 0.0
 Identities = 310/425 (72%), Positives = 352/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALSLWSFY+ASV AFGLL YVGYII+ FPS+F LHRLNGLLL+FIL WAV TYIFN
Sbjct: 328  VSLFALSLWSFYYASVCAFGLLGYVGYIIFVFPSLFRLHRLNGLLLVFILFWAVSTYIFN 387

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            + FS  N  LG    +WEMVGLWHYP PGFF+LAQFC               FLY+SDE 
Sbjct: 388  IVFSNQNGKLGKDTQVWEMVGLWHYPFPGFFILAQFCLGFLVALVNLVNNSVFLYLSDEV 447

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
               TS+GD+TPEV+EETKVLIVATIAW+LRKC+RAIMLV IFLIAMKPGFIHA+YM+FFF
Sbjct: 448  PS-TSDGDSTPEVKEETKVLIVATIAWVLRKCNRAIMLVQIFLIAMKPGFIHAVYMIFFF 506

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSH IS +V+  LLLLCEIHFA LY+LQI  VS AL KS +IS  ILSQLG  E +S
Sbjct: 507  LYLLSHRISPRVQGALLLLCEIHFAILYTLQINLVSGALRKSDTISWAILSQLGLLECDS 566

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDF+EIALLA FCAI KHG+K+LFSFSAIVQHTP  P+GFSILKAGLNKSVLLSVYA  
Sbjct: 567  TWDFVEIALLAGFCAIQKHGHKVLFSFSAIVQHTPRPPIGFSILKAGLNKSVLLSVYAVA 626

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
             ST  DDNPSQERKVASYLS++GQ+FLSLYRSFGTYIAF TIL+TVYMT PN+VSFG++F
Sbjct: 627  NSTHIDDNPSQERKVASYLSAIGQRFLSLYRSFGTYIAFVTILITVYMTPPNYVSFGYLF 686

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQLV+KT++RLW+PLK Y+I+MFI+ YILSIFP+ E W+STK DLY  LGY 
Sbjct: 687  LLLFWITGRQLVEKTRKRLWFPLKAYSIVMFILIYILSIFPSFEGWISTKVDLYKYLGYN 746

Query: 1262 PEGSL 1276
             E ++
Sbjct: 747  SEAAV 751


>ref|XP_016484083.1| PREDICTED: piezo-type mechanosensitive ion channel homolog, partial
            [Nicotiana tabacum]
          Length = 1888

 Score =  610 bits (1573), Expect = 0.0
 Identities = 294/425 (69%), Positives = 351/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 331  VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 391  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+   + EV+EETKVLIVAT+AW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 451  NQSSSDS-FSEEVKEETKVLIVATVAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 510  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 570  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYASS 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            +S  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 630  SSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 690  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 749

Query: 1262 PEGSL 1276
             E SL
Sbjct: 750  HEASL 754


>gb|KVH94432.1| Piezo family [Cynara cardunculus var. scolymus]
          Length = 2449

 Score =  617 bits (1591), Expect = 0.0
 Identities = 306/425 (72%), Positives = 356/425 (83%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFAL+LWSFY+AS+ AFGLL YVGY+I++FPS+FHLHRLNGLLL+FILLWAV TYIFN
Sbjct: 390  VSLFALALWSFYYASICAFGLLGYVGYVIFSFPSLFHLHRLNGLLLVFILLWAVSTYIFN 449

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            +AFS LNW +G   +IWEMVGLWHYP PGFF+LAQFC               FLYISDE 
Sbjct: 450  IAFSCLNWKVGKDTEIWEMVGLWHYPFPGFFILAQFCLGVLVSLVNLVNNSVFLYISDEV 509

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
             H T + D  PEVEEETKVLIVATIAW+LRKC+RAIMLV IFLIAMKP       ++FFF
Sbjct: 510  HH-TDDVDPAPEVEEETKVLIVATIAWVLRKCNRAIMLVQIFLIAMKP-------VIFFF 561

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            LYLLSH IS +V+Q+LLLLCEIHFA LY+LQIT +S   EKS + S EILSQLG  E +S
Sbjct: 562  LYLLSHRISTRVQQSLLLLCEIHFAILYTLQITLISGVREKSETTSWEILSQLGLLECDS 621

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             W F+EIALLACFCAI KHG+K+LFSFSAIVQHTP  P+GFSILKAGLNKSVLLSVYA+ 
Sbjct: 622  SWSFVEIALLACFCAIQKHGHKVLFSFSAIVQHTPRPPIGFSILKAGLNKSVLLSVYAN- 680

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
             +  +DDNPSQERKVASYLS++GQKFLSLYRSFGTYIAF TIL+TVYM+RPN+VSFG++F
Sbjct: 681  -NNEDDDNPSQERKVASYLSAIGQKFLSLYRSFGTYIAFVTILITVYMSRPNYVSFGYLF 739

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLLFWITGRQLV+KT++RLW+PLK+Y+I+MFI+ YILSIFP+ ESW+STK D+YT LGY 
Sbjct: 740  LLLFWITGRQLVEKTRKRLWFPLKVYSIVMFILIYILSIFPSFESWISTKVDIYTYLGYN 799

Query: 1262 PEGSL 1276
             E S+
Sbjct: 800  SETSV 804


>ref|XP_009770606.1| PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X3
            [Nicotiana sylvestris]
          Length = 2420

 Score =  610 bits (1573), Expect = 0.0
 Identities = 294/425 (69%), Positives = 351/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 331  VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 391  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+   + EV+EETKVLIVAT+AW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 451  NQSSSDS-FSEEVKEETKVLIVATVAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 510  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 570  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYASS 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            +S  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 630  SSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 690  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 749

Query: 1262 PEGSL 1276
             E SL
Sbjct: 750  HEASL 754


>dbj|GAU30701.1| hypothetical protein TSUD_39250 [Trifolium subterraneum]
          Length = 1540

 Score =  595 bits (1533), Expect = 0.0
 Identities = 295/424 (69%), Positives = 344/424 (81%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSL ALS WSF+FAS+ AFGLL YVGYIIYAFPS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 71   VSLLALSFWSFHFASLCAFGLLAYVGYIIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFN 130

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF+FLNW LG  M IWEMVGLWHYPIPGFFLLAQFC               FL +SDE 
Sbjct: 131  VAFTFLNWKLGRDMKIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDEG 190

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  TSNG ++ +V+ ETKVLIVATIAW LRKCSRAIML LIFLIA+KPGFIHA+YM+FF 
Sbjct: 191  EQ-TSNGCSSVKVDGETKVLIVATIAWGLRKCSRAIMLALIFLIAIKPGFIHAVYMIFFL 249

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSH+IS K+RQ L+LLCEIHFA LY LQI  VS ALEK GS+S+EIL QLGF E +S
Sbjct: 250  IYLLSHNISRKLRQALILLCEIHFALLYILQINLVSSALEKKGSVSMEILMQLGFLEEDS 309

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLE+ALLACFC IH HG++MLFSFSAI+QH P  P+GFSILKAGLNKSVLLSVY+S 
Sbjct: 310  AWDFLEVALLACFCTIHNHGFEMLFSFSAIIQHAPSPPIGFSILKAGLNKSVLLSVYSSS 369

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            +  ++D++ S ER++ASYLS++GQKFLS+YRS GTYIAF TIL TVYM +PN+ SFG+IF
Sbjct: 370  SVRNSDESFSYERRIASYLSAVGQKFLSIYRSCGTYIAFLTILFTVYMVKPNYTSFGYIF 429

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WITGRQLV++TK++LW PLK+YAI +FI  Y LS F +LE  +S   DLY  LGY 
Sbjct: 430  LLLLWITGRQLVERTKKQLWLPLKVYAISVFIFIYSLSSFSSLEMCLSRMIDLYFYLGYD 489

Query: 1262 PEGS 1273
             + S
Sbjct: 490  SKSS 493


>ref|XP_009770604.1| PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X1
            [Nicotiana sylvestris]
          Length = 2473

 Score =  610 bits (1573), Expect = 0.0
 Identities = 294/425 (69%), Positives = 351/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 331  VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 391  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+   + EV+EETKVLIVAT+AW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 451  NQSSSDS-FSEEVKEETKVLIVATVAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 510  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 570  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYASS 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            +S  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 630  SSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 690  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 749

Query: 1262 PEGSL 1276
             E SL
Sbjct: 750  HEASL 754


>gb|OIT06222.1| piezo-type mechanosensitive ion channel-like protein [Nicotiana
            attenuata]
          Length = 2212

 Score =  607 bits (1564), Expect = 0.0
 Identities = 293/425 (68%), Positives = 349/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 70   VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 129

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 130  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 189

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+     EV+EETKVLIVAT+AW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 190  NQSSSDSFGE-EVKEETKVLIVATVAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 248

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 249  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 308

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 309  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYASS 368

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            +S  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 369  SSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 428

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 429  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 488

Query: 1262 PEGSL 1276
               SL
Sbjct: 489  HGASL 493


>ref|XP_012847917.1| PREDICTED: LOW QUALITY PROTEIN: piezo-type mechanosensitive ion
            channel homolog, partial [Erythranthe guttata]
          Length = 1492

 Score =  593 bits (1528), Expect = 0.0
 Identities = 289/425 (68%), Positives = 342/425 (80%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL YVGY++YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 332  VSLFALSYWSFHFASICAFGLLAYVGYVLYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 391

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF+++NW LG  M+IWEMVGLWHYPIPGFFLLAQFC               FL +S+EE
Sbjct: 392  VAFAYVNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSNEE 451

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
                SN + T EV+E+ KVLIVATIAW LRK SR IML+LIFLIA KPG  HA+YMVFFF
Sbjct: 452  -RQVSNENKTEEVKEDAKVLIVATIAWGLRKSSRPIMLLLIFLIATKPGLAHAVYMVFFF 510

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
             YLLSH+I  ++RQ+L+LLCE HFA LY LQ++ VS+ LE+ GSIS+E+LSQLG  E +S
Sbjct: 511  AYLLSHNIDTRMRQSLILLCEAHFAVLYILQLSLVSRKLEQKGSISMEVLSQLGLLESDS 570

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIALLACFC IH HG++MLFSFSAIVQHTP  PVGFSILKAGLNKSVLLSVYA+ 
Sbjct: 571  SWDFLEIALLACFCTIHNHGFEMLFSFSAIVQHTPFPPVGFSILKAGLNKSVLLSVYATS 630

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
             +     N S ER+VA YLS++G+KFLS+YRSFGTYIAF TIL+ VY+ RPNF++FG+IF
Sbjct: 631  NTRDYSVNHSHERRVALYLSAVGEKFLSIYRSFGTYIAFLTILLAVYLVRPNFIAFGYIF 690

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLW+PLK YAI++F+  YILS+FPT E W+S K +LY   GY 
Sbjct: 691  LLLVWIIGRQLVEKTKRRLWFPLKAYAIMVFVFIYILSVFPTFEMWMSQKVNLYVCFGYN 750

Query: 1262 PEGSL 1276
             + SL
Sbjct: 751  SDASL 755


>ref|XP_018630180.1| PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X2
            [Nicotiana tomentosiformis]
          Length = 2420

 Score =  608 bits (1569), Expect = 0.0
 Identities = 295/425 (69%), Positives = 350/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 331  VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 391  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+   + EV+EETKVLIVATIAW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 451  NQSSSDS-FSDEVKEETKVLIVATIAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 510  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 570  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYAST 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            TS  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 630  TSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 690  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 749

Query: 1262 PEGSL 1276
               SL
Sbjct: 750  HGASL 754


>ref|XP_021834067.1| piezo-type mechanosensitive ion channel homolog isoform X2 [Prunus
            avium]
          Length = 2125

 Score =  604 bits (1558), Expect = 0.0
 Identities = 298/424 (70%), Positives = 344/424 (81%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FASV AFGLL YVGYIIYAFPS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 333  VSLFALSFWSFHFASVCAFGLLAYVGYIIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFN 392

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF+FLNW +G   DIWEMVGLWHYPIPGFFLLAQFC               FL +SDE 
Sbjct: 393  VAFAFLNWKIGKNTDIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDE- 451

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            D   SN ++T E E ETKVLIVATIAW LRK SRAIML LIFLIAMKPGFIHA+Y++FF 
Sbjct: 452  DGRFSNDNSTVEGEGETKVLIVATIAWGLRKSSRAIMLALIFLIAMKPGFIHAVYVIFFL 511

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSH+IS K+RQ+L+LLCE+HFA LY +QI P+S  LE+ GS+S E+LSQLG  +H S
Sbjct: 512  IYLLSHNISRKIRQSLILLCEVHFALLYIIQINPISDTLEQKGSLSAEVLSQLGLLQHES 571

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFL+IALLACFCAIH HG++MLFSFSAIVQHTP  PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 572  SWDFLKIALLACFCAIHNHGFEMLFSFSAIVQHTPSRPVGFSILKAGLNKSVLLSVYASS 631

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
                + DNPS ER++A +LS++GQKFLS+YRS GTYIAF TIL+TVY+ RPN+VSFG+IF
Sbjct: 632  AIKYSHDNPSYERRIALFLSAIGQKFLSVYRSCGTYIAFLTILLTVYLVRPNYVSFGYIF 691

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV++TK+RLW+PLK YAI++FI  Y LS F ++E W+S   DLY  LGY 
Sbjct: 692  LLLAWIIGRQLVERTKKRLWFPLKAYAIVVFIFIYSLSSFRSIEVWLSRLIDLYFYLGYD 751

Query: 1262 PEGS 1273
             E S
Sbjct: 752  SEAS 755


>ref|XP_009614902.1| PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X1
            [Nicotiana tomentosiformis]
          Length = 2473

 Score =  608 bits (1569), Expect = 0.0
 Identities = 295/425 (69%), Positives = 350/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 331  VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 391  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+   + EV+EETKVLIVATIAW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 451  NQSSSDS-FSDEVKEETKVLIVATIAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 510  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 570  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYAST 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            TS  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 630  TSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 690  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 749

Query: 1262 PEGSL 1276
               SL
Sbjct: 750  HGASL 754


>ref|XP_019228329.1| PREDICTED: piezo-type mechanosensitive ion channel homolog isoform X1
            [Nicotiana attenuata]
          Length = 2473

 Score =  607 bits (1564), Expect = 0.0
 Identities = 293/425 (68%), Positives = 349/425 (82%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FAS+ AFGLL Y+GYI+YAFPS+F LHRLNGLLL+FILLWAV TY+FN
Sbjct: 331  VSLFALSFWSFHFASICAFGLLAYIGYILYAFPSLFRLHRLNGLLLVFILLWAVSTYVFN 390

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            V F FLNW LG  M+IWEMVGLWHYPIPGFFLLAQF                FL +SDEE
Sbjct: 391  VGFVFLNWKLGKDMEIWEMVGLWHYPIPGFFLLAQFFLGILVAVGNLVNNSVFLCMSDEE 450

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            +  +S+     EV+EETKVLIVAT+AW LRKCSRAIMLVLIFLIA KPGFIHA+YMVFFF
Sbjct: 451  NQSSSDSFGE-EVKEETKVLIVATVAWGLRKCSRAIMLVLIFLIATKPGFIHAVYMVFFF 509

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSHDI+ K+RQ+L+LLCE HFA LY LQ+  +S+ LE+  S+S+ ILSQLG  + +S
Sbjct: 510  VYLLSHDINEKMRQSLILLCEAHFAILYILQLNLISKTLERESSLSMVILSQLGLLQSDS 569

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFLEIA+LACFCA+H HG+ +LFSFSAIVQHTPC PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 570  YWDFLEIAVLACFCAVHNHGFDVLFSFSAIVQHTPCPPVGFSILKAGLNKSVLLSVYASS 629

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
            +S  +D + S E+++ASYLS++G+KFLS+YRSFGTYIAF TIL+TVY+ +PN++SFG+IF
Sbjct: 630  SSRDHDHDHSHEKRIASYLSAIGKKFLSVYRSFGTYIAFVTILITVYLVKPNYISFGYIF 689

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV+KTKRRLWYPLKLY I++F++ Y LSIFPT E+W+S  FD+   LGY 
Sbjct: 690  LLLLWIIGRQLVEKTKRRLWYPLKLYGILVFVLIYSLSIFPTFEAWMSRNFDIQLYLGYD 749

Query: 1262 PEGSL 1276
               SL
Sbjct: 750  HGASL 754


>ref|XP_020425451.1| piezo-type mechanosensitive ion channel homolog isoform X2 [Prunus
            persica]
          Length = 2124

 Score =  602 bits (1552), Expect = 0.0
 Identities = 297/424 (70%), Positives = 344/424 (81%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FASV AFGLL YVGYIIYAFPS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 333  VSLFALSFWSFHFASVCAFGLLAYVGYIIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFN 392

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF+FLNW +G   DIWEMVGLWHYPIPGFFLLAQFC               FL +SDE 
Sbjct: 393  VAFAFLNWKIGKNTDIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDE- 451

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            D   SN ++T E E ETKVLIVATIAW LRK SRAIML+LIFLIAMKPGFIHA+Y++FF 
Sbjct: 452  DARFSNDNSTVEGEGETKVLIVATIAWGLRKSSRAIMLLLIFLIAMKPGFIHAVYVIFFL 511

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSH+IS K+RQ+L+LLCE+HFA LY +QI P+S  LE+ GS+S E+LSQLG  +H S
Sbjct: 512  IYLLSHNISRKIRQSLILLCEVHFALLYIIQINPISDTLERKGSLSAEVLSQLGLLQHES 571

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFL+IALLACFCAIH HG++MLFSFSAIVQHT   PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 572  SWDFLKIALLACFCAIHNHGFEMLFSFSAIVQHTSSRPVGFSILKAGLNKSVLLSVYASS 631

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
                + DNPS ER++A +LS++GQKFLS+YRS GTYIAF TIL+TVY+ RPN+VSFG+IF
Sbjct: 632  AIKYSHDNPSYERRIALFLSAIGQKFLSVYRSCGTYIAFLTILLTVYLVRPNYVSFGYIF 691

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV++TK+RLW+PLK YAI++FI  Y LS F ++E W+S   DLY  LGY 
Sbjct: 692  LLLTWIIGRQLVERTKKRLWFPLKAYAIVVFIFIYSLSSFRSIEVWLSRLIDLYFYLGYD 751

Query: 1262 PEGS 1273
             E S
Sbjct: 752  SEAS 755


>gb|ONH93697.1| hypothetical protein PRUPE_8G248000 [Prunus persica]
          Length = 2210

 Score =  602 bits (1552), Expect = 0.0
 Identities = 297/424 (70%), Positives = 344/424 (81%)
 Frame = +2

Query: 2    VSLFALSLWSFYFASVSAFGLLVYVGYIIYAFPSVFHLHRLNGLLLMFILLWAVMTYIFN 181
            VSLFALS WSF+FASV AFGLL YVGYIIYAFPS+F LHRLNGLLL+FILLWAV TYIFN
Sbjct: 58   VSLFALSFWSFHFASVCAFGLLAYVGYIIYAFPSLFRLHRLNGLLLVFILLWAVSTYIFN 117

Query: 182  VAFSFLNWDLGDGMDIWEMVGLWHYPIPGFFLLAQFCXXXXXXXXXXXXXXXFLYISDEE 361
            VAF+FLNW +G   DIWEMVGLWHYPIPGFFLLAQFC               FL +SDE 
Sbjct: 118  VAFAFLNWKIGKNTDIWEMVGLWHYPIPGFFLLAQFCLGILVALGNLVNNSVFLCLSDE- 176

Query: 362  DHPTSNGDATPEVEEETKVLIVATIAWILRKCSRAIMLVLIFLIAMKPGFIHAIYMVFFF 541
            D   SN ++T E E ETKVLIVATIAW LRK SRAIML+LIFLIAMKPGFIHA+Y++FF 
Sbjct: 177  DARFSNDNSTVEGEGETKVLIVATIAWGLRKSSRAIMLLLIFLIAMKPGFIHAVYVIFFL 236

Query: 542  LYLLSHDISNKVRQTLLLLCEIHFAFLYSLQITPVSQALEKSGSISLEILSQLGFYEHNS 721
            +YLLSH+IS K+RQ+L+LLCE+HFA LY +QI P+S  LE+ GS+S E+LSQLG  +H S
Sbjct: 237  IYLLSHNISRKIRQSLILLCEVHFALLYIIQINPISDTLERKGSLSAEVLSQLGLLQHES 296

Query: 722  PWDFLEIALLACFCAIHKHGYKMLFSFSAIVQHTPCTPVGFSILKAGLNKSVLLSVYASG 901
             WDFL+IALLACFCAIH HG++MLFSFSAIVQHT   PVGFSILKAGLNKSVLLSVYAS 
Sbjct: 297  SWDFLKIALLACFCAIHNHGFEMLFSFSAIVQHTSSRPVGFSILKAGLNKSVLLSVYASS 356

Query: 902  TSTSNDDNPSQERKVASYLSSMGQKFLSLYRSFGTYIAFATILVTVYMTRPNFVSFGFIF 1081
                + DNPS ER++A +LS++GQKFLS+YRS GTYIAF TIL+TVY+ RPN+VSFG+IF
Sbjct: 357  AIKYSHDNPSYERRIALFLSAIGQKFLSVYRSCGTYIAFLTILLTVYLVRPNYVSFGYIF 416

Query: 1082 LLLFWITGRQLVQKTKRRLWYPLKLYAIIMFIICYILSIFPTLESWVSTKFDLYTILGYK 1261
            LLL WI GRQLV++TK+RLW+PLK YAI++FI  Y LS F ++E W+S   DLY  LGY 
Sbjct: 417  LLLTWIIGRQLVERTKKRLWFPLKAYAIVVFIFIYSLSSFRSIEVWLSRLIDLYFYLGYD 476

Query: 1262 PEGS 1273
             E S
Sbjct: 477  SEAS 480