BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00029974
         (2378 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVI12435.1| Zinc finger, RING/FYVE/PHD-type [Cynara carduncul...   593   0.0  
ref|XP_021968981.1| putative E3 ubiquitin-protein ligase RF298 [...   554   0.0  
gb|OTG21756.1| putative zinc finger, RING/FYVE/PHD-type [Heliant...   554   0.0  
ref|XP_023749584.1| putative E3 ubiquitin-protein ligase RF298 [...   466   e-151
gb|PLY61778.1| hypothetical protein LSAT_8X55580 [Lactuca sativa]     463   e-150
ref|XP_015168014.1| PREDICTED: putative E3 ubiquitin-protein lig...   343   e-102
ref|XP_006355787.1| PREDICTED: putative E3 ubiquitin-protein lig...   343   e-102
gb|OAY61717.1| hypothetical protein MANES_01G211400 [Manihot esc...   322   6e-95
ref|XP_021623591.1| putative E3 ubiquitin-protein ligase RF298 [...   315   4e-92
ref|XP_024167144.1| putative E3 ubiquitin-protein ligase RF298 [...   315   1e-91
ref|XP_017616200.1| PREDICTED: putative E3 ubiquitin-protein lig...   304   2e-87
ref|XP_010522682.1| PREDICTED: putative E3 ubiquitin-protein lig...   300   4e-86
ref|XP_016717405.1| PREDICTED: putative E3 ubiquitin-protein lig...   300   9e-86
ref|NP_192209.2| RING/U-box superfamily protein [Arabidopsis tha...   297   6e-85
ref|XP_020876365.1| putative E3 ubiquitin-protein ligase RF298 i...   292   3e-83
ref|XP_010456961.1| PREDICTED: LOW QUALITY PROTEIN: putative E3 ...   289   5e-82
ref|XP_017186392.1| PREDICTED: putative E3 ubiquitin-protein lig...   287   5e-81
ref|XP_013469244.1| E3 ubiquitin-protein ligase RF298-like prote...   262   8e-73
ref|XP_003591611.2| E3 ubiquitin-protein ligase RF298-like prote...   262   1e-72
ref|XP_010430601.1| PREDICTED: putative E3 ubiquitin-protein lig...   259   6e-71

>gb|KVI12435.1| Zinc finger, RING/FYVE/PHD-type [Cynara cardunculus var. scolymus]
          Length = 669

 Score =  593 bits (1530), Expect = 0.0
 Identities = 333/672 (49%), Positives = 432/672 (64%), Gaps = 40/672 (5%)
 Frame = +3

Query: 201  MAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEV 380
            MAEMK+ L+ P+E+   L  D DWD ++VS L KL++D LH+C   A+K+IV NGY ++V
Sbjct: 1    MAEMKAKLNWPEEEDGELDSDYDWDCSSVSGLRKLMTDGLHSCFGNAVKKIVENGYPEQV 60

Query: 381  AEQTVLKCGPFYSAKDMVSSITDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTML 560
            AE  VLK GPF+ +KDMVS I D AL+ L+ +  +D+ +Y F++  +LV+FM+LEMVTML
Sbjct: 61   AEFVVLKSGPFFGSKDMVSVIVDRALNSLRTKGCHDSKFYIFEELHTLVEFMILEMVTML 120

Query: 561  RHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSAEPES----L 728
            R VKP LSVREA+WTLLICDLN+I AS+A+LDP KF I  +K+   +  S++ ES    +
Sbjct: 121  REVKPFLSVREALWTLLICDLNVIHASEAELDPMKFFIDSVKAAHEIETSSDSESQNQEI 180

Query: 729  PEPKTEPEPNTVPEPKTEPESKSAGKKVDEKCQCCTKCCTG--HKKS------FRFERTY 884
                 E +   V   K+ P+S  +GK++++KC  C K C+G  HKK       FRFE+ Y
Sbjct: 181  LASNVEKQDMVVKSLKSSPKSSKSGKRIEDKCPSCRKWCSGNSHKKDAVREKMFRFEKPY 240

Query: 885  SGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVNNKNPLPLKETNS--------EPV 1040
             GRMSKKALK  L +W   +L  +K   +CESSS       L LKET          EPV
Sbjct: 241  KGRMSKKALKEKLAAWG--DLVSDKKHPSCESSSGTKKTPTLALKETEETAKATPKPEPV 298

Query: 1041 ----SQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWAN 1208
                + EP KT ++YL+ IP+DE+KGEHV +D KD+++L A   + +L KEL+DWDDWAN
Sbjct: 299  APVAAPEPTKTVQHYLASIPYDESKGEHVPRDVKDKILLAAVDQVLALHKELRDWDDWAN 358

Query: 1209 VKVMQVAKRLSYDR-----------PXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXX 1355
            +KVMQVAKRLS DR                               SE T ALN       
Sbjct: 359  LKVMQVAKRLSQDRAELNKLRAEKEEAEKMMKDKQVAEENNMRKLSEMTTALNNANYQIE 418

Query: 1356 XXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCESFGPXXXXXXX 1535
                   RL+ E  +LK++ E A  RS  EA+ L+ ALQKEQEA +K +SFG        
Sbjct: 419  MASSAVVRLKSEKIILKKEMEAARIRSSIEAKRLEAALQKEQEAYKKSQSFGTEKSLLEE 478

Query: 1536 XXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKA 1715
                    VVP QR  EKAK LL++T+IRLA E+KETAK   + ES++ ERE +E +AK+
Sbjct: 479  ELKALKHEVVPIQRSAEKAKDLLSQTQIRLANEEKETAKVVLKYESLKRERELIEEMAKS 538

Query: 1716 EEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEMSWQPAELTSSQSEV 1895
            EE++  EKAE +L+++E+E+KR+Q EI A+KLE ETKKIAAMR  M+W P    S  SE+
Sbjct: 539  EEEMVVEKAEKKLQKFENEMKRIQYEISAMKLEQETKKIAAMRTGMNW-PVFEGSKSSEI 597

Query: 1896 -----KHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQ 2060
                 K+G    KKDRECVMCLTEEMTVV VPCGHQVLCA+CNV+HE++GMKDCPSCRT 
Sbjct: 598  LRNNNKNGNLRQKKDRECVMCLTEEMTVVLVPCGHQVLCADCNVIHEQNGMKDCPSCRTP 657

Query: 2061 IQKRITARFAKS 2096
            IQKRI ARFAKS
Sbjct: 658  IQKRINARFAKS 669


>ref|XP_021968981.1| putative E3 ubiquitin-protein ligase RF298 [Helianthus annuus]
 ref|XP_021968982.1| putative E3 ubiquitin-protein ligase RF298 [Helianthus annuus]
          Length = 600

 Score =  554 bits (1428), Expect = 0.0
 Identities = 328/632 (51%), Positives = 400/632 (63%), Gaps = 21/632 (3%)
 Frame = +3

Query: 264  SDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPFYSA--KDMVS 437
            S WD+TTV+DLAKL SD+LH C   AIK+I  NGYSK+ AE+TVLKCGPFY     DMVS
Sbjct: 10   SGWDNTTVADLAKLFSDTLHACFRNAIKKISQNGYSKDTAEETVLKCGPFYGGINDDMVS 69

Query: 438  SITDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLIC 617
            SI D ALDYL  + RN  TWY+FDD   LV++MMLEMVTML ++KPSLSV EAMWTLLIC
Sbjct: 70   SIADRALDYL--QIRNCHTWYRFDDIHRLVEYMMLEMVTMLTYIKPSLSVHEAMWTLLIC 127

Query: 618  DLNIIDASQADLDPQKFSIGGLKSGQGVGPSAEPESLPEPKTEPEPNTVPEPKTEPE--- 788
            DLN+  AS+A+ DP K  I                S P+ +TE E   +P P  E     
Sbjct: 128  DLNVHVASEAEFDPHKCFI------------RRSPSKPQTQTETE---IPSPDQEETIAA 172

Query: 789  ---SKSAGKKVDEKCQCCTKCCTGHKKSFR-FERTYSGRMSKKALKANLTSWKKLNLSIE 956
               +K  G+  DEKC+CC K C+G+K++   FE+ + G+MSKKALKA +     +NL   
Sbjct: 173  LNLTKPPGRS-DEKCRCCKKWCSGNKRNLTSFEKAFKGQMSKKALKAKI-----VNLVDN 226

Query: 957  KVQSACESSSSVNNKNPLP-LKETNSEPVSQEPLKTAEYYLSGIPFDEAKGEHVAQDDKD 1133
            K       S SV+ +NPL  ++E   +  + +P KTA+YYL+GIPFDE+KGEHV QDD+D
Sbjct: 227  K------QSPSVSKENPLKEIEEEKPKVTTPKPFKTADYYLAGIPFDESKGEHVPQDDQD 280

Query: 1134 EMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRP-----------XXXXXXXXX 1280
            +MILTA + LQSLQK++K WDDWA+VKVMQVA RLS D P                    
Sbjct: 281  KMILTAVAQLQSLQKQVKSWDDWASVKVMQVANRLSQDGPEIKKLKAEKVEAENLKKDKE 340

Query: 1281 XXXXXXXXXXSETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLK 1460
                      SE TAAL T             RLE ENSVLK+D+E+AE RSL EA SL 
Sbjct: 341  ATEESTKKKLSEMTAALTTTDCQFKMSTSTIARLESENSVLKRDKERAEKRSLIEANSLA 400

Query: 1461 EALQKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKK 1640
            EAL KE E ++K E  G                V P QRDL+KAK LL ETE RLA+E++
Sbjct: 401  EALCKELEVLKKSELCGSEKKLLEEELKSLKQEVGPNQRDLDKAKLLLKETETRLAQEER 460

Query: 1641 ETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAE 1820
            E AKF + A+SIR+ERE LEALA+A  +   ++A  ELK+ E+EIKRL+ EI A+ LEAE
Sbjct: 461  EAAKFYEEAKSIRNERERLEALAEAGGERAMKRAAKELKKVETEIKRLEYEIAALNLEAE 520

Query: 1821 TKKIAAMRKEMSWQPAELTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCA 2000
            TKKIA     MSWQ             G +    D ECVMCL+EEMTV+FVPCGHQVLC 
Sbjct: 521  TKKIAG---GMSWQ------------LGCEGLSHD-ECVMCLSEEMTVLFVPCGHQVLCD 564

Query: 2001 ECNVLHEKSGMKDCPSCRTQIQKRITARFAKS 2096
            ECNV+HE +GMK+CPSCRT IQKRI   FAKS
Sbjct: 565  ECNVIHEDNGMKECPSCRTPIQKRINCLFAKS 596


>gb|OTG21756.1| putative zinc finger, RING/FYVE/PHD-type [Helianthus annuus]
          Length = 620

 Score =  554 bits (1428), Expect = 0.0
 Identities = 328/632 (51%), Positives = 400/632 (63%), Gaps = 21/632 (3%)
 Frame = +3

Query: 264  SDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPFYSA--KDMVS 437
            S WD+TTV+DLAKL SD+LH C   AIK+I  NGYSK+ AE+TVLKCGPFY     DMVS
Sbjct: 30   SGWDNTTVADLAKLFSDTLHACFRNAIKKISQNGYSKDTAEETVLKCGPFYGGINDDMVS 89

Query: 438  SITDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLIC 617
            SI D ALDYL  + RN  TWY+FDD   LV++MMLEMVTML ++KPSLSV EAMWTLLIC
Sbjct: 90   SIADRALDYL--QIRNCHTWYRFDDIHRLVEYMMLEMVTMLTYIKPSLSVHEAMWTLLIC 147

Query: 618  DLNIIDASQADLDPQKFSIGGLKSGQGVGPSAEPESLPEPKTEPEPNTVPEPKTEPE--- 788
            DLN+  AS+A+ DP K  I                S P+ +TE E   +P P  E     
Sbjct: 148  DLNVHVASEAEFDPHKCFI------------RRSPSKPQTQTETE---IPSPDQEETIAA 192

Query: 789  ---SKSAGKKVDEKCQCCTKCCTGHKKSFR-FERTYSGRMSKKALKANLTSWKKLNLSIE 956
               +K  G+  DEKC+CC K C+G+K++   FE+ + G+MSKKALKA +     +NL   
Sbjct: 193  LNLTKPPGRS-DEKCRCCKKWCSGNKRNLTSFEKAFKGQMSKKALKAKI-----VNLVDN 246

Query: 957  KVQSACESSSSVNNKNPLP-LKETNSEPVSQEPLKTAEYYLSGIPFDEAKGEHVAQDDKD 1133
            K       S SV+ +NPL  ++E   +  + +P KTA+YYL+GIPFDE+KGEHV QDD+D
Sbjct: 247  K------QSPSVSKENPLKEIEEEKPKVTTPKPFKTADYYLAGIPFDESKGEHVPQDDQD 300

Query: 1134 EMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRP-----------XXXXXXXXX 1280
            +MILTA + LQSLQK++K WDDWA+VKVMQVA RLS D P                    
Sbjct: 301  KMILTAVAQLQSLQKQVKSWDDWASVKVMQVANRLSQDGPEIKKLKAEKVEAENLKKDKE 360

Query: 1281 XXXXXXXXXXSETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLK 1460
                      SE TAAL T             RLE ENSVLK+D+E+AE RSL EA SL 
Sbjct: 361  ATEESTKKKLSEMTAALTTTDCQFKMSTSTIARLESENSVLKRDKERAEKRSLIEANSLA 420

Query: 1461 EALQKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKK 1640
            EAL KE E ++K E  G                V P QRDL+KAK LL ETE RLA+E++
Sbjct: 421  EALCKELEVLKKSELCGSEKKLLEEELKSLKQEVGPNQRDLDKAKLLLKETETRLAQEER 480

Query: 1641 ETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAE 1820
            E AKF + A+SIR+ERE LEALA+A  +   ++A  ELK+ E+EIKRL+ EI A+ LEAE
Sbjct: 481  EAAKFYEEAKSIRNERERLEALAEAGGERAMKRAAKELKKVETEIKRLEYEIAALNLEAE 540

Query: 1821 TKKIAAMRKEMSWQPAELTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCA 2000
            TKKIA     MSWQ             G +    D ECVMCL+EEMTV+FVPCGHQVLC 
Sbjct: 541  TKKIAG---GMSWQ------------LGCEGLSHD-ECVMCLSEEMTVLFVPCGHQVLCD 584

Query: 2001 ECNVLHEKSGMKDCPSCRTQIQKRITARFAKS 2096
            ECNV+HE +GMK+CPSCRT IQKRI   FAKS
Sbjct: 585  ECNVIHEDNGMKECPSCRTPIQKRINCLFAKS 616


>ref|XP_023749584.1| putative E3 ubiquitin-protein ligase RF298 [Lactuca sativa]
          Length = 663

 Score =  466 bits (1199), Expect = e-151
 Identities = 294/674 (43%), Positives = 399/674 (59%), Gaps = 38/674 (5%)
 Frame = +3

Query: 189  KEKYMAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGY 368
            ++K M  MK+ L + KE++EA   D D D+T V  L KL+S++L+ C+  AI +I   GY
Sbjct: 4    EDKVMESMKAKLVMTKEEVEAFLTDPDCDTTGVRGLIKLVSETLNLCIQNAITKITKKGY 63

Query: 369  SKEVAEQTVLKCGP---FYSAKDMVSSITDCALDYLKKETR--NDATWYKFDDFDSLVKF 533
            +KE AE  +L+CGP   FY ++ + S+I +CAL  LK   +  ND+  Y F + + LV F
Sbjct: 64   TKEAAENAILRCGPLTPFYCSEGVESNIAECALLSLKSNNKKYNDSADYTFQELNELVDF 123

Query: 534  MMLEMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSA 713
             +LEMV  LR  KP+LSV EA+W LL+CDLN+  AS  + D Q+           +G SA
Sbjct: 124  TVLEMVATLRDSKPNLSVSEAIWCLLMCDLNVSKASVMERDLQQ-----------MGTSA 172

Query: 714  EPESLPEPKTEPE-PNTVPEPKTEPESKSA--GKKVDEKCQCCTKCCTG-HKK------- 860
            EPE     K+EP+   T+      P S+S+  GKK D+ C C   C    HK+       
Sbjct: 173  EPEIESGSKSEPKNQETIASNVKRPPSQSSKSGKKTDDICTCRNWCAVATHKRDATLRGK 232

Query: 861  -SFRFERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVN---NKNP-LPLKET 1025
             +   E+ Y GR  +K LK  + S K+L+L  +K  S+  SSSS +   NKN  L LKET
Sbjct: 233  AALLVEKPYKGRRIRKGLKKQVFSLKELSLVNKKSPSSSSSSSSSSSEQNKNTSLALKET 292

Query: 1026 NSEPV----SQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDW 1193
              +P     S++P KT +YYL  IP DE KGE+V +DDKD + L A + +++LQKEL+ W
Sbjct: 293  EEKPKGTPKSEQP-KTLDYYLKNIPCDE-KGEYVPRDDKDILRLEAVAQIEALQKELQGW 350

Query: 1194 DDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXXS----ETTAALNTXXXXXXXX 1361
            DDWA  KVM+V KRL+ +R                    +    ETT  LNT        
Sbjct: 351  DDWATSKVMEVTKRLNQNRSEVNMLKAEMKDNEAMDENTAKRLLETTLTLNTTNSELNVA 410

Query: 1362 XXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCES-FGPXXXXXXXX 1538
                 +L+ E SV+K++ E+  NRS+ +A+ L++AL KE+EA +K +S FG         
Sbjct: 411  NSTIVKLKTEQSVIKEEMERERNRSIFQAKRLEQALIKEEEARKKSQSAFGSEKTSLEEE 470

Query: 1539 XXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAE 1718
                   V  KQ++LEKAK LL+ET IR+AKE++ETAK  ++AE I++ERE +EALA AE
Sbjct: 471  LKVLKRQVGQKQQELEKAKCLLSETLIRVAKEERETAKVVNKAECIKNERERIEALAMAE 530

Query: 1719 EQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEMSW--------QPAEL 1874
             +I   KAE E K+ E+E KR++ EI A+KLEAE+K+IA M   M+         +    
Sbjct: 531  YEIMKGKAEEEFKKNETEKKRIEYEISALKLEAESKRIAEMYMSMNMNMDSSKGLEGFRN 590

Query: 1875 TSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCR 2054
             S + + K   K  KKDRECVMCL EEM+VVFVPCGHQVLC  CNV HE+  M +CPSCR
Sbjct: 591  MSKKKKKKKKMKKKKKDRECVMCLNEEMSVVFVPCGHQVLCKACNVKHEER-MNECPSCR 649

Query: 2055 TQIQKRITARFAKS 2096
            T IQKRI ARFAKS
Sbjct: 650  TPIQKRINARFAKS 663


>gb|PLY61778.1| hypothetical protein LSAT_8X55580 [Lactuca sativa]
          Length = 656

 Score =  463 bits (1192), Expect = e-150
 Identities = 293/670 (43%), Positives = 396/670 (59%), Gaps = 38/670 (5%)
 Frame = +3

Query: 201  MAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEV 380
            M  MK+ L + KE++EA   D D D+T V  L KL+S++L+ C+  AI +I   GY+KE 
Sbjct: 1    MESMKAKLVMTKEEVEAFLTDPDCDTTGVRGLIKLVSETLNLCIQNAITKITKKGYTKEA 60

Query: 381  AEQTVLKCGP---FYSAKDMVSSITDCALDYLKKETR--NDATWYKFDDFDSLVKFMMLE 545
            AE  +L+CGP   FY ++ + S+I +CAL  LK   +  ND+  Y F + + LV F +LE
Sbjct: 61   AENAILRCGPLTPFYCSEGVESNIAECALLSLKSNNKKYNDSADYTFQELNELVDFTVLE 120

Query: 546  MVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSAEPES 725
            MV  LR  KP+LSV EA+W LL+CDLN+  AS  + D Q+           +G SAEPE 
Sbjct: 121  MVATLRDSKPNLSVSEAIWCLLMCDLNVSKASVMERDLQQ-----------MGTSAEPEI 169

Query: 726  LPEPKTEPE-PNTVPEPKTEPESKSA--GKKVDEKCQCCTKCCTG-HKK--------SFR 869
                K+EP+   T+      P S+S+  GKK D+ C C   C    HK+        +  
Sbjct: 170  ESGSKSEPKNQETIASNVKRPPSQSSKSGKKTDDICTCRNWCAVATHKRDATLRGKAALL 229

Query: 870  FERTYSGRMSKKALKANLTSWKKLNLSIEKVQSACESSSSVN---NKNP-LPLKETNSEP 1037
             E+ Y GR  +K LK  + S K+L+L  +K  S+  SSSS +   NKN  L LKET  +P
Sbjct: 230  VEKPYKGRRIRKGLKKQVFSLKELSLVNKKSPSSSSSSSSSSSEQNKNTSLALKETEEKP 289

Query: 1038 V----SQEPLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWA 1205
                 S++P KT +YYL  IP DE KGE+V +DDKD + L A + +++LQKEL+ WDDWA
Sbjct: 290  KGTPKSEQP-KTLDYYLKNIPCDE-KGEYVPRDDKDILRLEAVAQIEALQKELQGWDDWA 347

Query: 1206 NVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXXS----ETTAALNTXXXXXXXXXXXX 1373
              KVM+V KRL+ +R                    +    ETT  LNT            
Sbjct: 348  TSKVMEVTKRLNQNRSEVNMLKAEMKDNEAMDENTAKRLLETTLTLNTTNSELNVANSTI 407

Query: 1374 FRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCES-FGPXXXXXXXXXXXX 1550
             +L+ E SV+K++ E+  NRS+ +A+ L++AL KE+EA +K +S FG             
Sbjct: 408  VKLKTEQSVIKEEMERERNRSIFQAKRLEQALIKEEEARKKSQSAFGSEKTSLEEELKVL 467

Query: 1551 XXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQIT 1730
               V  KQ++LEKAK LL+ET IR+AKE++ETAK  ++AE I++ERE +EALA AE +I 
Sbjct: 468  KRQVGQKQQELEKAKCLLSETLIRVAKEERETAKVVNKAECIKNERERIEALAMAEYEIM 527

Query: 1731 AEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEMSW--------QPAELTSSQ 1886
              KAE E K+ E+E KR++ EI A+KLEAE+K+IA M   M+         +     S +
Sbjct: 528  KGKAEEEFKKNETEKKRIEYEISALKLEAESKRIAEMYMSMNMNMDSSKGLEGFRNMSKK 587

Query: 1887 SEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQ 2066
             + K   K  KKDRECVMCL EEM+VVFVPCGHQVLC  CNV HE+  M +CPSCRT IQ
Sbjct: 588  KKKKKKMKKKKKDRECVMCLNEEMSVVFVPCGHQVLCKACNVKHEER-MNECPSCRTPIQ 646

Query: 2067 KRITARFAKS 2096
            KRI ARFAKS
Sbjct: 647  KRINARFAKS 656


>ref|XP_015168014.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X2
            [Solanum tuberosum]
          Length = 819

 Score =  343 bits (879), Expect = e-102
 Identities = 246/766 (32%), Positives = 369/766 (48%), Gaps = 143/766 (18%)
 Frame = +3

Query: 234  KEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPF 413
            K+ +E L   +DWD T    L +LL  +L     +AIK+IV  GYS+E+AE+ +L+ G +
Sbjct: 56   KQGVETL-QQADWDDTIACQLMELLFHNLSATFQSAIKRIVECGYSEEIAERVLLRSGLY 114

Query: 414  YSAKDMVSSITDCALDYLKKETRND-ATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVR 590
            + +KD VS+I D AL  L +E   D +T   F+D +SLV++ MLEM+ +LR VKP  +V 
Sbjct: 115  HGSKDAVSTIMDGALALLSREKELDTSTSLIFEDLNSLVEYTMLEMICVLREVKPDFTVA 174

Query: 591  EAMWTLLICDLNIIDASQADLDPQKFSIGG---LKSGQGVGPSAEPE------------- 722
            EAMW LLICDLN++ A   + D Q  S       KS  G    A+P+             
Sbjct: 175  EAMWCLLICDLNLLHACSIERDLQVDSCSSESPRKSSSG-SKLAQPKEAFVVSQLGLDKL 233

Query: 723  -----SLPEPKTEPEPNTVPEPKTE----------------------------PESKSAG 803
                 S+P  K+        +P T+                             +SKSAG
Sbjct: 234  QLSKPSMPIAKSLQSETPCDDPVTQFAQLSNSIYSHLHGVEITANRSSARLPVAKSKSAG 293

Query: 804  KKVDEKCQCCT------KCCTGHK-------------KSFRFERTYSGRMSKKALKANLT 926
               +             KC TG +             K+F FE++Y GRM K + KA LT
Sbjct: 294  VSGESVLSITKAAILEEKCGTGRRGSSLNSKKDMLRQKTFHFEKSYKGRMGKGSFKAKLT 353

Query: 927  SWKKLNLS---------------------IEKVQSAC-----ESSSSVNNKNPLPLKETN 1028
            +W  + L                         V+S C      S SS  + +  P  ET 
Sbjct: 354  TWSSMVLDKTLNSESRSSGLGMKSTNSKVTTTVKSNCPLAVGSSHSSSTSPSIAPSSETA 413

Query: 1029 SEPVSQEPL--------------------------------KTAEYYLSGIPFDEAKGEH 1112
            + P +Q+ +                                K  +YY +GIP+DE+  +H
Sbjct: 414  TVPATQDTVCALPAVNTNIAASLTPDPKSSSNTPGSTPALPKVLDYY-AGIPYDESLEKH 472

Query: 1113 VAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDR-----------PXX 1259
            V Q++KD+++L   + +Q+LQKEL+ W DWAN KVMQ A+RL  D+              
Sbjct: 473  VPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVMQAARRLGKDQGELKMLKQEKEEAE 532

Query: 1260 XXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSL 1439
                             SE   AL+              RL+ EN  LK++ + A   +L
Sbjct: 533  KLQKEKQIVEENNMKRLSEMEYALSNASGQSKMADSTLHRLKEENVSLKKEMDAATLAAL 592

Query: 1440 TEAQSLKEALQKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEI 1619
              A +  +A+ +EQE ++KC+++                  V  +++LE+AK    + ++
Sbjct: 593  ESATNFHQAVAREQEILKKCQAWEMKKGSLLDNFSTLKREAVHFEQELERAKTRQNQLKV 652

Query: 1620 RLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREID 1799
               +E++E  K   +A+S+++ERE     +K EE    EKAE  +++ + +I++L+ EI 
Sbjct: 653  LSEQEEREKRKVLQQADSLKAEREKRGVQSKMEEDNIREKAERNMQKCKEDIRKLESEIS 712

Query: 1800 AIKLEAETKKIAAMRKEMSW---QPAELTSSQS--EVKHGTKSNKKDRECVMCLTEEMTV 1964
             ++ ++E  KI A+R+ ++    Q  +LT S +  E   G+ S K +RECVMCL+EEMTV
Sbjct: 713  LLRFQSEGSKIEALRRGINNTRPQSPKLTKSLAVFEENLGSGSVKIERECVMCLSEEMTV 772

Query: 1965 VFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAKS*R 2102
            VF+PC HQVLCA+CNVLHEK GM DCPSCRT I+KRI  +FA+S R
Sbjct: 773  VFLPCAHQVLCAQCNVLHEKQGMNDCPSCRTPIKKRINVQFARSSR 818


>ref|XP_006355787.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum tuberosum]
 ref|XP_006355788.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Solanum tuberosum]
          Length = 845

 Score =  343 bits (879), Expect = e-102
 Identities = 246/766 (32%), Positives = 369/766 (48%), Gaps = 143/766 (18%)
 Frame = +3

Query: 234  KEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPF 413
            K+ +E L   +DWD T    L +LL  +L     +AIK+IV  GYS+E+AE+ +L+ G +
Sbjct: 82   KQGVETL-QQADWDDTIACQLMELLFHNLSATFQSAIKRIVECGYSEEIAERVLLRSGLY 140

Query: 414  YSAKDMVSSITDCALDYLKKETRND-ATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVR 590
            + +KD VS+I D AL  L +E   D +T   F+D +SLV++ MLEM+ +LR VKP  +V 
Sbjct: 141  HGSKDAVSTIMDGALALLSREKELDTSTSLIFEDLNSLVEYTMLEMICVLREVKPDFTVA 200

Query: 591  EAMWTLLICDLNIIDASQADLDPQKFSIGG---LKSGQGVGPSAEPE------------- 722
            EAMW LLICDLN++ A   + D Q  S       KS  G    A+P+             
Sbjct: 201  EAMWCLLICDLNLLHACSIERDLQVDSCSSESPRKSSSG-SKLAQPKEAFVVSQLGLDKL 259

Query: 723  -----SLPEPKTEPEPNTVPEPKTE----------------------------PESKSAG 803
                 S+P  K+        +P T+                             +SKSAG
Sbjct: 260  QLSKPSMPIAKSLQSETPCDDPVTQFAQLSNSIYSHLHGVEITANRSSARLPVAKSKSAG 319

Query: 804  KKVDEKCQCCT------KCCTGHK-------------KSFRFERTYSGRMSKKALKANLT 926
               +             KC TG +             K+F FE++Y GRM K + KA LT
Sbjct: 320  VSGESVLSITKAAILEEKCGTGRRGSSLNSKKDMLRQKTFHFEKSYKGRMGKGSFKAKLT 379

Query: 927  SWKKLNLS---------------------IEKVQSAC-----ESSSSVNNKNPLPLKETN 1028
            +W  + L                         V+S C      S SS  + +  P  ET 
Sbjct: 380  TWSSMVLDKTLNSESRSSGLGMKSTNSKVTTTVKSNCPLAVGSSHSSSTSPSIAPSSETA 439

Query: 1029 SEPVSQEPL--------------------------------KTAEYYLSGIPFDEAKGEH 1112
            + P +Q+ +                                K  +YY +GIP+DE+  +H
Sbjct: 440  TVPATQDTVCALPAVNTNIAASLTPDPKSSSNTPGSTPALPKVLDYY-AGIPYDESLEKH 498

Query: 1113 VAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDR-----------PXX 1259
            V Q++KD+++L   + +Q+LQKEL+ W DWAN KVMQ A+RL  D+              
Sbjct: 499  VPQNEKDKIVLLRIARIQTLQKELQGWTDWANEKVMQAARRLGKDQGELKMLKQEKEEAE 558

Query: 1260 XXXXXXXXXXXXXXXXXSETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSL 1439
                             SE   AL+              RL+ EN  LK++ + A   +L
Sbjct: 559  KLQKEKQIVEENNMKRLSEMEYALSNASGQSKMADSTLHRLKEENVSLKKEMDAATLAAL 618

Query: 1440 TEAQSLKEALQKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEI 1619
              A +  +A+ +EQE ++KC+++                  V  +++LE+AK    + ++
Sbjct: 619  ESATNFHQAVAREQEILKKCQAWEMKKGSLLDNFSTLKREAVHFEQELERAKTRQNQLKV 678

Query: 1620 RLAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREID 1799
               +E++E  K   +A+S+++ERE     +K EE    EKAE  +++ + +I++L+ EI 
Sbjct: 679  LSEQEEREKRKVLQQADSLKAEREKRGVQSKMEEDNIREKAERNMQKCKEDIRKLESEIS 738

Query: 1800 AIKLEAETKKIAAMRKEMSW---QPAELTSSQS--EVKHGTKSNKKDRECVMCLTEEMTV 1964
             ++ ++E  KI A+R+ ++    Q  +LT S +  E   G+ S K +RECVMCL+EEMTV
Sbjct: 739  LLRFQSEGSKIEALRRGINNTRPQSPKLTKSLAVFEENLGSGSVKIERECVMCLSEEMTV 798

Query: 1965 VFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAKS*R 2102
            VF+PC HQVLCA+CNVLHEK GM DCPSCRT I+KRI  +FA+S R
Sbjct: 799  VFLPCAHQVLCAQCNVLHEKQGMNDCPSCRTPIKKRINVQFARSSR 844


>gb|OAY61717.1| hypothetical protein MANES_01G211400 [Manihot esculenta]
          Length = 730

 Score =  322 bits (824), Expect = 6e-95
 Identities = 215/651 (33%), Positives = 322/651 (49%), Gaps = 41/651 (6%)
 Frame = +3

Query: 264  SDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPFYSAKDMVSSI 443
            +DW       L +LL  ++ T    AIKQ+  +G+S+ VA++ + + G +   +D+V++I
Sbjct: 88   TDWSDPLAFHLEELLLFNIRTIFQNAIKQLCLSGFSEHVAQRAITRLGLYQGGRDIVANI 147

Query: 444  TDCALDYLKKETRNDATW-YKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICD 620
               AL  L      D+T    F++   +V++ MLE+V ++R VKPSLS  EAMW LLICD
Sbjct: 148  VTDALALLTSGKDIDSTKDIVFENLQQMVEYTMLELVNVVRQVKPSLSTGEAMWRLLICD 207

Query: 621  LNIIDASQADLDPQKFSIGGLKSGQGVGPSAEPESLPEPKT-----EPEPNTVPEPKTEP 785
            +NI  A  A+ D     +G        G S+   SLP+ ++     EP P++ P  +T  
Sbjct: 208  MNISQACAAEGD----LLGECGCKAIPGASSSNSSLPKSRSKAQCSEPIPSSNPSSRTSQ 263

Query: 786  ESKSAGKKVDEKCQCCTKCCTGHKKSFRFERTY-----------------SGRMSKKALK 914
             S S GK    +     K      K+   ++T                  S  +  K+  
Sbjct: 264  TSPSEGKSKKARKSRSKKELAARNKTLNPDKTKGSASNTKDKVEAPVREGSSHVPNKSPS 323

Query: 915  ANLTSWKKLNLSIEKVQSACESSSSVNNKNPLPLKETNSEPVSQEPLKTAEYYLSGIPFD 1094
            A   S     +  E   SA  +  +V+ K PL   E N+        K ++YY S IP+D
Sbjct: 324  AVSASDDPSKVHKEGTTSAANTELTVSGKKPLLKPEANTAVFP----KISDYY-SRIPYD 378

Query: 1095 EAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXX 1274
            E+ G+++ QD+KDE+IL     +Q LQ EL+ W DWAN KV Q   RLS D+P       
Sbjct: 379  ESLGKYIPQDEKDELILKLVPQVQELQNELQSWTDWANQKVRQATHRLSKDKPELKALRL 438

Query: 1275 XXXXXXXXXXXX----SETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLT 1442
                            SE   AL               +LE E S+L  + +  + +++ 
Sbjct: 439  EKEEKKTLEENTLRRISEMELALVNAAGQVEAANSTICKLEEEQSLLHDEFDTVKLQAVE 498

Query: 1443 EAQSLKEALQKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIR 1622
               + +EAL++E++A++  +S+                 V   QR + +A+ +    E R
Sbjct: 499  ADVNCEEALEREKKALKDAQSWKGQKSLLQEELQTHKQRVTELQRKIGEAEKIKNHFEAR 558

Query: 1623 LAKEKKETAKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDA 1802
              +E+    K    A SIR+ERE LEA AKA E I  +KAE +LK+Y   I +L++EI  
Sbjct: 559  WEQERMLNEKLLAEAASIRNEREQLEAAAKAGEDIIRQKAEEDLKKYMENISKLEKEISK 618

Query: 1803 IKLEAETKKIAAMRKEM--------------SWQPAELTSSQSEVKHGTKSNKKDRECVM 1940
            ++L ++  KIAA+R+ +              S     L  +  +   G+   K+DRECVM
Sbjct: 619  LRLTSDASKIAALRRSVDGNYGGTSIQSMKGSQDSNVLRKADFQETCGSGGLKRDRECVM 678

Query: 1941 CLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            CL+EE  VVF+PC HQVLC +CN +H K GMKDCPSCRT IQ+RI ARFA+
Sbjct: 679  CLSEEKCVVFLPCAHQVLCTKCNEIHGKQGMKDCPSCRTPIQRRIAARFAR 729


>ref|XP_021623591.1| putative E3 ubiquitin-protein ligase RF298 [Manihot esculenta]
          Length = 763

 Score =  315 bits (807), Expect = 4e-92
 Identities = 218/679 (32%), Positives = 327/679 (48%), Gaps = 69/679 (10%)
 Frame = +3

Query: 264  SDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPFYSAKDMVSSI 443
            +DW       L +LL  ++ T    AIKQ+  +G+S+ VA++ + + G +   +D+V++I
Sbjct: 88   TDWSDPLAFHLEELLLFNIRTIFQNAIKQLCLSGFSEHVAQRAITRLGLYQGGRDIVANI 147

Query: 444  TDCALDYLKKETRNDATW-YKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICD 620
               AL  L      D+T    F++   +V++ MLE+V ++R VKPSLS  EAMW LLICD
Sbjct: 148  VTDALALLTSGKDIDSTKDIVFENLQQMVEYTMLELVNVVRQVKPSLSTGEAMWRLLICD 207

Query: 621  LNIIDASQADLDPQKFSIGGLKSGQGVGPSAEPESLPEPKT-----EPEPNTVPEPKTEP 785
            +NI  A  A+ D     +G        G S+   SLP+ ++     EP P++ P  +T  
Sbjct: 208  MNISQACAAEGD----LLGECGCKAIPGASSSNSSLPKSRSKAQCSEPIPSSNPSSRTSQ 263

Query: 786  ESKSAGKKVDEKCQCCTKCCTGHKKSFRFERT--YSGRMSKKALKANLTSWKKLNLSIE- 956
             S S GK    +     K      K+   ++     G+  K A    L    ++  S E 
Sbjct: 264  TSPSEGKSKKARKSRSKKELAARNKTLNPDKVPGNKGKSGKAASSGRLIMENRMKSSSES 323

Query: 957  --------------KVQSAC-ESSSSVNNKNP----------------------LPLKET 1025
                          KV++   E SS V NK+P                        L  +
Sbjct: 324  HGLQTKGSASNTKDKVEAPVREGSSHVPNKSPSAVSASDDPSKVHKEGTTSAANTELTVS 383

Query: 1026 NSEPVSQEPLKTAEY-----YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKD 1190
              +P+ +    TA +     Y S IP+DE+ G+++ QD+KDE+IL     +Q LQ EL+ 
Sbjct: 384  GKKPLLKPEANTAVFPKISDYYSRIPYDESLGKYIPQDEKDELILKLVPQVQELQNELQS 443

Query: 1191 WDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX----SETTAALNTXXXXXXX 1358
            W DWAN KV Q   RLS D+P                       SE   AL         
Sbjct: 444  WTDWANQKVRQATHRLSKDKPELKALRLEKEEKKTLEENTLRRISEMELALVNAAGQVEA 503

Query: 1359 XXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCESFGPXXXXXXXX 1538
                  +LE E S+L  + +  + +++    + +EAL++E++A++  +S+          
Sbjct: 504  ANSTICKLEEEQSLLHDEFDTVKLQAVEADVNCEEALEREKKALKDAQSWKGQKSLLQEE 563

Query: 1539 XXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAE 1718
                   V   QR + +A+ +    E R  +E+    K    A SIR+ERE LEA AKA 
Sbjct: 564  LQTHKQRVTELQRKIGEAEKIKNHFEARWEQERMLNEKLLAEAASIRNEREQLEAAAKAG 623

Query: 1719 EQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEM--------------S 1856
            E I  +KAE +LK+Y   I +L++EI  ++L ++  KIAA+R+ +              S
Sbjct: 624  EDIIRQKAEEDLKKYMENISKLEKEISKLRLTSDASKIAALRRSVDGNYGGTSIQSMKGS 683

Query: 1857 WQPAELTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMK 2036
                 L  +  +   G+   K+DRECVMCL+EE  VVF+PC HQVLC +CN +H K GMK
Sbjct: 684  QDSNVLRKADFQETCGSGGLKRDRECVMCLSEEKCVVFLPCAHQVLCTKCNEIHGKQGMK 743

Query: 2037 DCPSCRTQIQKRITARFAK 2093
            DCPSCRT IQ+RI ARFA+
Sbjct: 744  DCPSCRTPIQRRIAARFAR 762


>ref|XP_024167144.1| putative E3 ubiquitin-protein ligase RF298 [Rosa chinensis]
 ref|XP_024167145.1| putative E3 ubiquitin-protein ligase RF298 [Rosa chinensis]
 gb|PRQ26203.1| putative transcription factor C2H2 family [Rosa chinensis]
          Length = 818

 Score =  315 bits (807), Expect = 1e-91
 Identities = 240/769 (31%), Positives = 354/769 (46%), Gaps = 133/769 (17%)
 Frame = +3

Query: 186  KKEKYMAEMKSTLDLPKEDME--ALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVA 359
            +K      +++T+  PKE+ E  A+    DWD      L +LL   L     +AIK+I  
Sbjct: 51   RKRSEPEPVEATVVQPKEEAETGAVEEGRDWDDPIACQLEELLLSYLQAIFQSAIKRITE 110

Query: 360  NGYSKEVAEQTVLKCGPFYSAKDMVSSITDCALDYLKKETRNDATWY-KFDDFDSLVKFM 536
             GYS+EVA + V + G +   KD V +I +  L++LK     DA+   +FD+   +V++ 
Sbjct: 111  CGYSEEVAVKAVSRGGLYVGGKDSVLNIVNDTLEFLKGGKDTDASRENEFDNLQHVVEYT 170

Query: 537  MLEMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSAE 716
            MLEM+++LR V+PSLSV EAMW LLICDLNI+ A   + D          S +    S+ 
Sbjct: 171  MLEMISVLREVRPSLSVAEAMWWLLICDLNILLACTMEGDLSSVLAFEEASSESSSDSSS 230

Query: 717  PESLPEPKTE---PEPNTV------------------------------PE-PKTEPESK 794
             +   E  TE   P P+                                PE P+ + ES 
Sbjct: 231  SQLKSEANTEMNHPNPSNANSSKPSLPNSQNHRSLSETLKFGSFPNSANPETPEGKEESL 290

Query: 795  SAGKKVDEKCQCCTK---------------------------CCTGHKKSFRFERTYSGR 893
            S+     EKC   TK                                K+SF+ ER Y   
Sbjct: 291  SSMLDSLEKCLGSTKDYVLSSSQTSTSEEKSGAVRKGRTKAELAALRKRSFQMERNYRAY 350

Query: 894  MSKKALKAN---------LTSWKKLNLSIEKVQSACESSSSVNNKNP------LPLKETN 1028
             SK   K+          +    K    +  V +  +SSS V   +       +P K T 
Sbjct: 351  RSKGGFKSGKLAAFGSFVMEKRPKPPSDLPGVHTK-DSSSKVTGSSARGCLSTVPSKGTT 409

Query: 1029 SE----------------------PVSQE-PLKTAEYYLSGIPFDEAKGEHVAQDDKDEM 1139
            S                       PVS   P KT +Y  +GIP+D++ G++V QD KDE+
Sbjct: 410  SSLPSVDTKQKQKSDSEEKSDTKTPVSTSVPPKTIDY-CAGIPYDKSLGKYVPQDKKDEL 468

Query: 1140 ILTATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXXS-- 1313
            IL     LQ LQ EL+ W +WAN KVMQV++RL  +RP                      
Sbjct: 469  ILKLVPRLQKLQDELQSWTEWANEKVMQVSRRLGKERPELKALRQEKEEAGKFEKEKQVV 528

Query: 1314 ---------ETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEA 1466
                     E   ALN              RL+  NS LK+    A+  ++  A +L+EA
Sbjct: 529  EENTMKRLFEMEHALNNASSQVESANSTISRLQVNNSTLKKQLSSAKIMAIESAANLQEA 588

Query: 1467 LQKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKET 1646
            L++E +A++K +++                 V   Q+DL KAK  L + E R  +E  E 
Sbjct: 589  LERENKALKKVQAWEGQKGSLQEELETKKNKVAALQQDLGKAKNALHQIEARWKREIMEK 648

Query: 1647 AKFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETK 1826
                 +A+S+R+ERE  EAL + EE     KAE+++++Y  EIK L+ ++  ++ ++++ 
Sbjct: 649  ETILAQADSMRNEREQREALTQVEEAKIKHKAEDDMQKYVEEIKLLESKLSELQTKSDSS 708

Query: 1827 KIAAMRKEMSWQPAELTSSQSEVKH--------------------GTKSNKKDRECVMCL 1946
            +IAA+R++ +       S +  VK                     GT+S ++DRECVMCL
Sbjct: 709  RIAALRRDAAGSFGACLSDRKNVKPTKGSQSSTSFKKLTNSRDHLGTESLRQDRECVMCL 768

Query: 1947 TEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            +EEM+VVF+PC HQV+CA+CN LH K GMKDCPSCR  IQ+RI  ++A+
Sbjct: 769  SEEMSVVFLPCAHQVVCAKCNELHLKQGMKDCPSCRALIQRRIKVQYAR 817


>ref|XP_017616200.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Gossypium
            arboreum]
 gb|KHG14418.1| hypothetical protein F383_03946 [Gossypium arboreum]
          Length = 837

 Score =  304 bits (779), Expect = 2e-87
 Identities = 231/753 (30%), Positives = 342/753 (45%), Gaps = 143/753 (18%)
 Frame = +3

Query: 264  SDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPFYSAKDMVSSI 443
            +DWD      L +LL  +L      AIK+IV  G+ ++VAE+ + + G +   KD+VS++
Sbjct: 85   ADWDDPIACQLEELLLSNLQMIFRNAIKKIVECGFKEDVAEKAISRHGLYQGGKDLVSNV 144

Query: 444  TDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICDL 623
             + A+  LKK    D + + F+D   LV + MLEM+++LR VKP LS+ EAMW LL+ DL
Sbjct: 145  VNDAIASLKKGIEGDISIHLFEDLQQLVVYTMLEMISVLREVKPCLSIAEAMWWLLMFDL 204

Query: 624  NIIDA-------------------SQADLDPQ----------KFSIGGLKSGQGVGPSAE 716
            NI  A                   S +D +P+          K SI    +G    PS  
Sbjct: 205  NISVACEVEGDILRNLGCVEVSGESSSDSNPKSRVGTQNPETKLSISNESNGSK--PSLS 262

Query: 717  PESL--------PEPKTEP-----------------EPNTVPEPKTEPESKSAGKKVDEK 821
            PE+L        P+PK+ P                   + VP      E    G+K   K
Sbjct: 263  PEALKFGSFPNLPKPKSPPPYEGMTPEKEFLVSRSVSGDYVPVASISEEKTGIGRKGRSK 322

Query: 822  CQCCTKCCTGHKKSFRFERTYSG-----RMSKKALKANLTSWKKLN-------------- 944
             +         KKSF  E   +      R  K A   +    K++               
Sbjct: 323  KELAAV----RKKSFNMEINRAAYWKSFRAVKLAASGSSVVEKRMKSPSELPAVHMKDSS 378

Query: 945  ---------LSIEKVQSACESSSSVNNKNPLPLKETNSEP---VSQEPL----------- 1055
                     L+ E   +A +SS+    K+ +P   T   P   +  +P+           
Sbjct: 379  PNVVMEAGALADESHHAADDSSTLKGTKSTVPTANTGLAPSLSLENKPVPKSKGGTSKSS 438

Query: 1056 KTAEY----------------------YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQS 1169
            KT E                       Y +GIP+DE+ G+++ Q++KDEMIL     ++ 
Sbjct: 439  KTPEQDAGKKPTPKAKSSTSMSSKSIDYCAGIPYDESLGKYIPQNEKDEMILKLVPRIEE 498

Query: 1170 LQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------SE 1316
            LQ EL  W  W N KVMQ A+RLS D+                               SE
Sbjct: 499  LQNELDSWTQWTNQKVMQAARRLSKDQAELKSLRQEKEEVEQLKREKEIMEENTMKRLSE 558

Query: 1317 TTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRK 1496
               ALN              +LE E+S+LK + E A+++++  A S +EAL++EQ+A++ 
Sbjct: 559  MEFALNNATSQVEDANNTVQKLEVEHSMLKMEMEVAKSQAIASAVSCREALEREQKALKD 618

Query: 1497 CESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESI 1676
             +S+                     +R + KA+ + ++ E+   +E+    KF  +A SI
Sbjct: 619  IQSWEGQRSLLQEELASEKQKATELKRKVGKAQNIYSQIEMTWKEERMAKEKFLAQAASI 678

Query: 1677 RSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEMS 1856
            R ERE LEA+AK EE     KAE ++K+Y  EIK L+ ++  +K++ ++ KIAA+R  + 
Sbjct: 679  RKERERLEAVAKVEEDKIKLKAEKDMKKYGEEIKMLENKLSELKMKLDSSKIAALRGCID 738

Query: 1857 WQPAELTS------SQSEVKH--------GTKSNKKDRECVMCLTEEMTVVFVPCGHQVL 1994
                + +S      S S  K         G++  K++RECVMCL+EE  VVF+PC HQVL
Sbjct: 739  GGNGQCSSVNDGYHSPSFSKRVVDINDYSGSRGLKQERECVMCLSEEKIVVFLPCAHQVL 798

Query: 1995 CAECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            C +CN LHEK  MKDCP+CRT I  RI ARFAK
Sbjct: 799  CVKCNELHEKQRMKDCPACRTVISNRICARFAK 831


>ref|XP_010522682.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Tarenaya
            hassleriana]
 ref|XP_010522683.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Tarenaya
            hassleriana]
          Length = 807

 Score =  300 bits (768), Expect = 4e-86
 Identities = 222/735 (30%), Positives = 341/735 (46%), Gaps = 111/735 (15%)
 Frame = +3

Query: 222  LDLPKEDMEALAHDSD---WDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQT 392
            L + K   ++   DS+   WD      L +LLS +L T   +AIKQIV  GY ++V  + 
Sbjct: 73   LSMEKLSSQSKVEDSESVGWDDPLACHLQELLSSNLATLFHSAIKQIVDCGYREDVVLKA 132

Query: 393  VLKCGPFYSAKDMVSSITDCALDYLKKETRNDATWYK--FDDFDSLVKFMMLEMVTMLRH 566
            +   G +    D+VS++ +  ++++K   +  A W    F+D + LV + ++EM+ ++R 
Sbjct: 133  ITGSGLYCGGNDLVSNLVNDTVNFMKSG-KEGAGWRDTVFEDLEQLVAYTLVEMINLVRE 191

Query: 567  VKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSAE---PESLPEP 737
            ++PSLS  EAMW LL+CDLN++ A + D D  + S G   S       AE   P+S    
Sbjct: 192  IRPSLSTVEAMWQLLMCDLNVLLACELDGDGLEGSSGFSLSESSESLGAECNTPKSSDPD 251

Query: 738  KTEPEPNT----------------VPEPKTEPES-KSAGKKVDEKCQCC----------- 833
              +P P+T                +P PK    S K+ GK++                  
Sbjct: 252  NQKPPPSTSHNNQSDSLKFGTFPYLPNPKNSRASGKTPGKEIFSGSSASGEGIKSASLTS 311

Query: 834  ---TKCCTGHKKSFRFE-------------RTYS-GRMSKKALKANLTSW-------KKL 941
                K  TG K   + E             RTYS G   K A  A++ S+        K+
Sbjct: 312  ISDDKLVTGRKGRTKKEMAMLRQKSCVEKIRTYSKGSGYKTAKFASVGSFLVDKRVKSKM 371

Query: 942  NLSIEKVQSACESSSSVNNKN---------------------------PLPLKETNSEPV 1040
               I       +S ++++N +                           PL   E NS   
Sbjct: 372  TAEIGVKVPLSDSGNTISNNSSKSDPPAGDAKGCVTALPATSADSSTPPLSTSEKNSSSE 431

Query: 1041 SQE----PLKTAEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDWAN 1208
            S++     +K A+ Y +GIP+D + G +V ++ KDE+IL      + LQ EL+ W +WAN
Sbjct: 432  SEQNASVSVKPAQDYYAGIPYDASLGGYVPRNKKDELILKLVPRAKELQNELQIWTEWAN 491

Query: 1209 VKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------SETTAALNTXXXXXX 1355
             KV +   RL  D+P                              SE   ALN       
Sbjct: 492  KKVKEATSRLLKDQPEIKALRKEKEEAEQYKREKQLVEENTTKRMSEMEFALNNATKQLE 551

Query: 1356 XXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCESFGPXXXXXXX 1535
                   RLE E ++LK++ E A+ R++  A+S +EA ++ Q+A++  +++         
Sbjct: 552  KANKTVRRLEVEQTLLKKEMEAAKVRAVESAESCREAKERGQKALKNAQAWEGQKVLLQE 611

Query: 1536 XXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKA 1715
                    V   Q+++  AK    + E    +EK    K   +A + + ERE +EA    
Sbjct: 612  ELKGQRGKVAELQQEVSMAKSRRCQIEGTWKQEKAAKEKLLAQAAATKKEREQVEAFGST 671

Query: 1716 EEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEM---------SWQPA 1868
            EE+    KAEN++K Y   IKRL+ EI  +KL+++  KIA ++K +         S  P 
Sbjct: 672  EEERIKRKAENDMKYYVENIKRLEGEISRLKLKSDCLKIAELKKGIDGSHGSKSGSLNPG 731

Query: 1869 ELTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPS 2048
            E   + +    G    K++RECVMCL+EEMTV+F+PC HQVLC++CN LHEK GM+DCPS
Sbjct: 732  ESGETNAWENLGETKIKRERECVMCLSEEMTVIFLPCAHQVLCSKCNQLHEKEGMEDCPS 791

Query: 2049 CRTQIQKRITARFAK 2093
            CR  IQ+RI ARFA+
Sbjct: 792  CRATIQRRIHARFAR 806


>ref|XP_016717405.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Gossypium
            hirsutum]
          Length = 837

 Score =  300 bits (767), Expect = 9e-86
 Identities = 228/751 (30%), Positives = 340/751 (45%), Gaps = 141/751 (18%)
 Frame = +3

Query: 264  SDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANGYSKEVAEQTVLKCGPFYSAKDMVSSI 443
            +DWD      L +LL  +L      AIK+IV  G+ ++VAE+ + + G +   KD+VS++
Sbjct: 85   ADWDDPIACQLEELLLSNLQMIFRNAIKKIVECGFKEDVAEKAISRHGLYQGGKDLVSNV 144

Query: 444  TDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICDL 623
             + A+  LKK    D + + F+D   LV + MLEM+++LR VKP LS+ EAMW LL+ DL
Sbjct: 145  VNDAIASLKKGIEGDISIHLFEDLQQLVVYTMLEMISVLREVKPCLSIAEAMWWLLMFDL 204

Query: 624  NIIDA-------------------SQADLDPQ----------KFSIGGLKSGQGVGPSAE 716
            NI  A                   S +D +P+          K SI    +G     S+E
Sbjct: 205  NISVACEVEGDILRNLGCVEVSGESSSDSNPKSRAGTQNPETKLSISNESNGSKPSLSSE 264

Query: 717  P------ESLPEPKTEP-----------------EPNTVPEPKTEPESKSAGKKVDEKCQ 827
                    +LP+PK+ P                   + VP      E    G+K   K +
Sbjct: 265  ALKFGSFPNLPKPKSPPPYEGMTPEKEFLVSRSVSGDYVPVASISEEKTGIGRKGRSKKE 324

Query: 828  CCTKCCTGHKKSFRFERTYSG-----RMSKKALKANLTSWKKLN---------------- 944
                     KKSF  E   +      R  K A   +    K++                 
Sbjct: 325  LAAV----RKKSFNMEINRAAYWKSFRAVKLAASGSSVVEKRMKSPSELPAVHMKDSLPN 380

Query: 945  -------LSIEKVQSACESSSSVNNKNPLPLKETNSEP---VSQEPL-----------KT 1061
                   L+ E   +A +SS+    K+ +P   T   P   +  +P+           KT
Sbjct: 381  VVMEAGALADESHHAADDSSTLKGTKSTVPTANTGLAPSLSLENKPVPKSKGGTSKSSKT 440

Query: 1062 AEY----------------------YLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQ 1175
             E                       Y +GIP+DE+ G+++ Q++KDEMIL     ++ LQ
Sbjct: 441  PEQDAGNKPTPKAKSSTSMSSKSIDYCAGIPYDESLGKYIPQNEKDEMILKLVPRIEELQ 500

Query: 1176 KELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------SETT 1322
             EL  W  W N KVMQ A+RLS D+                               SE  
Sbjct: 501  NELDSWTQWTNQKVMQAARRLSKDQAELKSLRQEKEEVEQLKREKEIMEENTMKRLSEME 560

Query: 1323 AALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCE 1502
             ALN              +LE E+S+LK + E A+++++  A S +EAL++EQ+A++  +
Sbjct: 561  FALNNATSQVEDANNTVQKLEVEHSMLKMEMEVAKSQAIASAVSCREALEREQKALKDIQ 620

Query: 1503 SFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRS 1682
            S+                     +R + KA+ + ++ E+   +E+    KF  +A SIR 
Sbjct: 621  SWEGQRSLLQEELASEKQKATELKRKVGKAQNIYSQIEMTWKEERMAKEKFLAQAASIRK 680

Query: 1683 EREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEMSWQ 1862
            ERE LEA+AK EE     KAE ++K+Y  EIK L+ ++  +K++ ++ KIAA+   +   
Sbjct: 681  ERERLEAVAKVEEDKIKLKAEKDMKKYGEEIKMLENKLSELKMKLDSSKIAALWGCIDGG 740

Query: 1863 PAELTS------SQSEVKH--------GTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCA 2000
              + +S      S S  K         G++  K++RECVMCL+EE  VVF+PC HQVLC 
Sbjct: 741  NGQCSSVNDRYHSPSFSKRVVDINDYSGSRGLKQERECVMCLSEEKIVVFLPCAHQVLCV 800

Query: 2001 ECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            +CN LHEK  MKDCP+CRT I  RI ARFAK
Sbjct: 801  KCNELHEKQRMKDCPACRTVISNRICARFAK 831


>ref|NP_192209.2| RING/U-box superfamily protein [Arabidopsis thaliana]
 ref|NP_974504.1| RING/U-box superfamily protein [Arabidopsis thaliana]
 sp|Q0WPJ7.1|RF298_ARATH RecName: Full=Putative E3 ubiquitin-protein ligase RF298; AltName:
            Full=RING finger protein 298; AltName: Full=RING-type E3
            ubiquitin transferase RF298
 dbj|BAF00952.1| hypothetical protein [Arabidopsis thaliana]
 gb|AEE82258.1| RING/U-box superfamily protein [Arabidopsis thaliana]
 gb|AEE82259.1| RING/U-box superfamily protein [Arabidopsis thaliana]
 gb|OAO97081.1| hypothetical protein AXX17_AT4G03690 [Arabidopsis thaliana]
          Length = 814

 Score =  297 bits (760), Expect = 6e-85
 Identities = 229/760 (30%), Positives = 346/760 (45%), Gaps = 124/760 (16%)
 Frame = +3

Query: 186  KKEKYMAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVANG 365
            K +  + E  S   L  E+ +++     WD      L  LLS +L T   +A+ QI+  G
Sbjct: 62   KSQSPLCENDSNGQLKAEESDSVG----WDDPFACHLEGLLSSNLLTLFRSAMNQIMDCG 117

Query: 366  YSKEVAEQTVLKCGPFYSAKDMVSSITDCALDYLKKETRNDATW-YKFDDFDSLVKFMML 542
            YS++V  + +     +    D+VS+I +  L +LK   +   +  Y F+D   LV + ++
Sbjct: 118  YSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFEDLQQLVAYSLV 177

Query: 543  EMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSAE-- 716
            E ++++R V+PSLS  EAMW LLICDLN++ A + D D  + S     S     P AE  
Sbjct: 178  EKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDADGLEGSSVSNASKSSESPVAECN 237

Query: 717  -PESLPEPKTEPEPNTVPEPKTEP-----------------ESKSAGKKV---------- 812
             P+S      +   +     ++EP                    + GK+V          
Sbjct: 238  PPKSSDADNPKAPVSNTQSKQSEPVKFGNFANVNNSKNPHASGATPGKEVFSVSTASGEG 297

Query: 813  ----------DEKCQCCTKCCTGHKKSFRFE-------RTYS------------------ 887
                      DEK   C K  T  + +   +       RTYS                  
Sbjct: 298  TKSASLTSVSDEKLVSCRKGRTKKEMAMLRQKSCVEKIRTYSKGGGYKTAKFGGFLVEKR 357

Query: 888  GRMSKKALKANL-TSWKKLNLSIEKVQSACESSSSVNNKN-------------------- 1004
            G+ +   L A    S  K+   + K+  A ESSS+++N                      
Sbjct: 358  GKSASDLLSAQARNSSSKITTEVMKIPLA-ESSSTLSNSTKSDSPALDVKEHVTALPANN 416

Query: 1005 -PLPL---KETNSEPVSQEPLKT--AEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQ 1166
             P P+   K++ SEP  +  + T  A  Y + IP+D   G ++ ++ +DE+IL     ++
Sbjct: 417  APAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVPRMK 476

Query: 1167 SLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------S 1313
             LQKEL+DW DWAN KV Q   RL  D+P                              S
Sbjct: 477  DLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIKRRS 536

Query: 1314 ETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMR 1493
            E   ALN              RLE E S+LK++RE A  R+   A+S +EA ++ Q  ++
Sbjct: 537  EMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQRLLK 596

Query: 1494 KCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAES 1673
              +S+                 V   Q+++ KAK    + E    +EK  T K   +A +
Sbjct: 597  NSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIEATWKQEKSATGKLTAQAAA 656

Query: 1674 IRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEM 1853
            ++ ER  LE L KAEE+    KAEN++K Y   IKRL  EI  +KL++++ KIAA++K +
Sbjct: 657  LKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDSLKIAALKKGI 716

Query: 1854 SWQPAELTSSQSEVKH--GTKSN------------------KKDRECVMCLTEEMTVVFV 1973
                     ++S + H   TK+N                  K++RECVMCL+EEM+V+F+
Sbjct: 717  D---GNNDGNKSGMNHTTNTKANSMASAKVWENNQGAESKIKRERECVMCLSEEMSVIFL 773

Query: 1974 PCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            PC HQVLC++CN LHEK  M+DCPSCR +IQ+RI ARFA+
Sbjct: 774  PCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813


>ref|XP_020876365.1| putative E3 ubiquitin-protein ligase RF298 isoform X1 [Arabidopsis
            lyrata subsp. lyrata]
          Length = 809

 Score =  292 bits (748), Expect = 3e-83
 Identities = 226/754 (29%), Positives = 336/754 (44%), Gaps = 118/754 (15%)
 Frame = +3

Query: 183  LKKEKYMAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVAN 362
            LK +  ++E  S   L  E+ +++     WD      L +LLS +L T   +A+ QI+  
Sbjct: 58   LKSQSPLSENDSNGQLKAEESDSVG----WDDPFACQLEQLLSSNLLTLFRSAMNQIMDC 113

Query: 363  GYSKEVAEQTVLKCGPFYSAKDMVSSITDCALDYLKK-ETRNDATWYKFDDFDSLVKFMM 539
            GYS++V  + +     +    D+VS+I +  L  LK  +    +  Y F+D   LV + +
Sbjct: 114  GYSEDVVLKAISSSRLYCGGNDLVSNIVNDTLSILKSGKNVAGSRDYVFEDLQQLVAYTL 173

Query: 540  LEMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGG------------- 680
            +E ++++R V+PSLS  EAMW LL+CDLN++ A + + D  + S G              
Sbjct: 174  VEKISLVREVRPSLSTVEAMWRLLMCDLNVLQAFEVEGDGLEGSSGSNASKSLESPVSEC 233

Query: 681  --LKSGQGVGPSAEPESLPEPKTEPE-----PNTVPEPKTEPESKSAGKKV--------- 812
               KS     P A   +    ++EP      PN            + GK+V         
Sbjct: 234  NPSKSSGSDNPKAPISNAQSNQSEPVKFGNFPNVNNSKNPHASGATPGKEVFSVSTASGE 293

Query: 813  -----------DEKCQCCTKCCTGHKKSFRFE-------RTYS-----------GRMSKK 905
                       DEK   C K  T  + +   +       RTYS           G + +K
Sbjct: 294  GTKSASLTSVSDEKLVSCRKGRTKKEMAMLRQKSCVEKIRTYSKGGGYKTAKFGGFLVEK 353

Query: 906  ALKANL--------TSWKKLNLSIEKVQSACESSSSVNN-KNPLPL-------------- 1016
              KA           S  K+   + K+  A  SS+  NN K+  P               
Sbjct: 354  RSKAASDLLSAQARNSSSKITTDVMKIPLAESSSTLSNNTKSDSPALDVKEHVTALPANN 413

Query: 1017 --------KETNSEPVSQEPLKT--AEYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQ 1166
                    K++ SEP  +  + T  A  Y + IP+D + G +V ++  DE+IL     ++
Sbjct: 414  APATVASEKKSGSEPEEKASVSTKPAPDYCAAIPYDASLGIYVPRNKGDELILKLVPRMK 473

Query: 1167 SLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX-----------S 1313
             LQKEL+DW DWAN KV Q   RL  D+P                              S
Sbjct: 474  DLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTMKRRS 533

Query: 1314 ETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMR 1493
            E   ALN              RLE E S+LK++RE A  R+   A+S +EA ++ Q  ++
Sbjct: 534  EMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRAAESAESCREAKERVQRLLK 593

Query: 1494 KCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAES 1673
              +S+                 V   Q+++ KAK    + E    +EK  T K   +A +
Sbjct: 594  NAQSWEGQKVLLQEELKSQKDKVAELQQEVAKAKTRQNQIEATWKQEKAATGKLTTQAAA 653

Query: 1674 IRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEM 1853
            ++ ER  LE L KAEE+    KAEN++K Y   IKRL  EI  +KL+++  KIAA++K +
Sbjct: 654  LKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDCLKIAALKKGI 713

Query: 1854 SWQ---------------PAELTSSQSEVKHGTKSNKKDRECVMCLTEEMTVVFVPCGHQ 1988
                              P   T             K++RECVMCL+EEM+V+F+PC HQ
Sbjct: 714  DGSNDKSGMNHTTTTKANPMAATKVWENNHRAESKIKRERECVMCLSEEMSVIFLPCAHQ 773

Query: 1989 VLCAECNVLHEKSGMKDCPSCRTQIQKRITARFA 2090
            VLC++CN LHEK  M+DCPSCR +IQ+RI ARF+
Sbjct: 774  VLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFS 807


>ref|XP_010456961.1| PREDICTED: LOW QUALITY PROTEIN: putative E3 ubiquitin-protein ligase
            RF298 [Camelina sativa]
          Length = 822

 Score =  289 bits (740), Expect = 5e-82
 Identities = 228/763 (29%), Positives = 345/763 (45%), Gaps = 126/763 (16%)
 Frame = +3

Query: 183  LKKEKYMAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVAN 362
            LK +  + E  S   L  E+ E+L     WD      L +LLS +L     +A+ QI+  
Sbjct: 65   LKSQNPLCENDSNGXLKAEESESLG----WDDPFACQLEQLLSSNLLNLFRSAMNQIMDC 120

Query: 363  GYSKEVAEQTVLKCGPFYSAKDMVSSITDCALDYLKKETRNDATW-YKFDDFDSLVKFMM 539
            GYS++V  + +     +    D+VS+I +  L +LK   +   +  Y F+D   LV + +
Sbjct: 121  GYSEDVVLKAISSSRLYCGGNDLVSNIVNDTLSFLKSGKKVAGSRDYVFEDLQQLVAYTL 180

Query: 540  LEMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIG-------GLKSGQG 698
            +E ++++R V+PSLS  EAMW LL+CDLN++ A + + +  + S G         +  +G
Sbjct: 181  VEKISLVREVRPSLSTVEAMWRLLMCDLNVLQAFEVEGEGMEGSSGCNANKTSETQGAEG 240

Query: 699  VGPSAEPESLPEPKTEPE---------------PNTVPEPKTEPESKSAGKKV------- 812
            + P  +P     PK+                  PN            + GK+V       
Sbjct: 241  IPP--KPTDSDNPKSPVSNTQCNHSDPVKFGNFPNVNNSKNPHASGATPGKEVFSVTNAS 298

Query: 813  -------------DEKCQCCTKCCTG-------HKKSFRFERTYS-----------GRMS 899
                         DEK   C K  T         K      RTYS           G + 
Sbjct: 299  AEGTKSASLTSVSDEKLVSCRKGRTKKELAMLRQKSCVEKIRTYSKGGGYKTAKFGGFLV 358

Query: 900  KKALKA--NLTSWKKLNLS-------IEKVQSACESSSSVNNKN---------------- 1004
            +K  KA  +L S +  N S       + K+  A   SS+++  N                
Sbjct: 359  EKRSKAASDLLSAQARNSSSKITTTEVVKIPLAESRSSTLSTSNSTKSDSPALDAKEYVT 418

Query: 1005 -------PLPL---KETNSEPVSQEPLKT--AEYYLSGIPFDEAKGEHVAQDDKDEMILT 1148
                   P P+   K+++S+P  +  + T  A  Y + IP+D + G +V ++ +DE+IL 
Sbjct: 419  ALPANNAPAPVASEKKSSSQPEEKASVSTKPAPDYYAAIPYDASLGIYVPRNKRDELILK 478

Query: 1149 ATSLLQSLQKELKDWDDWANVKVMQVAKRLSYDRPXXXXXXXXXXXXXXXXXXX------ 1310
                ++ LQKEL+DW DWAN KV Q   RL  D+P                         
Sbjct: 479  LVPRMKDLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEEN 538

Query: 1311 -----SETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQK 1475
                 SE   ALN              RLE E S+LK++RE A  R+   A+S +EA ++
Sbjct: 539  TMKRRSEMELALNNATNQIERANNTVRRLELEQSLLKREREAANIRAAESAESCREAKER 598

Query: 1476 EQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKF 1655
             Q  ++  +S+                 V   Q+++ KAK    + E    +EK    K 
Sbjct: 599  LQRLLKNAQSWEGQKVSLQEELKSQRHKVAELQQEVAKAKTRQNQIEATWKQEKAAKVKL 658

Query: 1656 RDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIA 1835
              +A +++ ER  LE   KAEE+    KAEN++K Y   IKRL+ EI  ++L+++  KIA
Sbjct: 659  AAQAAALKKERGKLEEFGKAEEERIKTKAENDVKYYIENIKRLESEISKLELKSDGLKIA 718

Query: 1836 AMRKEMSWQPAELTSSQS----------------EVKHGTKSN-KKDRECVMCLTEEMTV 1964
            A++K +        S  +                E  HG +S  K++RECVMCL+EEM+V
Sbjct: 719  ALKKGIDGSNDGNKSGMNHTTTTKANTMAGAKVWENNHGEESKIKRERECVMCLSEEMSV 778

Query: 1965 VFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            VF+PC HQVLC++CN LHEK  M+DCPSCR +IQ+RI ARFA+
Sbjct: 779  VFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 821


>ref|XP_017186392.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Malus
            domestica]
          Length = 850

 Score =  287 bits (734), Expect = 5e-81
 Identities = 237/811 (29%), Positives = 363/811 (44%), Gaps = 165/811 (20%)
 Frame = +3

Query: 153  TFDAKLSGKELKKEKYMAEMKSTLDLPKEDMEALAHD-SDWDSTTVSDLAKLLSDSLHTC 329
            T  ++L  +E +K       ++ +  PKE++E    + +DWD   V  L +LL   L   
Sbjct: 42   TSQSELPSEECQKLSDNESAEAGVVQPKEEVEVGDFECADWDDPIVCQLEELLLSYLQAI 101

Query: 330  LDTAIKQIVANGYSKEVAEQTVLKCGPFYSAKDMVSSITDCALDYLKKETRNDATWYKFD 509
              +AIKQI  +GYS+EVAE+ V + G +   KD + +I    L++LK E   DA+  +F 
Sbjct: 102  FRSAIKQIAEHGYSEEVAERAVSRGGLYIGGKDPLENIVSDTLEFLKTEKDIDASNDQFV 161

Query: 510  DFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKS 689
            +   LV++ MLEM++++R V+P LSV EAMW LLICDLNI+ A  ++ DP   ++G  +S
Sbjct: 162  NLQHLVEYTMLEMISVVREVRPLLSVAEAMWWLLICDLNILLACTSEGDPFS-ALGFEES 220

Query: 690  GQGVGP----------SAEPESLPEPKTEPEP---------NTVPEPKT-EPESKSA--- 800
                 P          S++P      K  PE          +  PE  T E +S S+   
Sbjct: 221  SYSSSPQLSLKADEANSSKPPPAHAQKNPPETLKFGSFRKHSHAPEEVTSEKDSLSSMLD 280

Query: 801  ---------GKKVDEKCQCCT--------------KCCTGHKKSFRFERTYS-------- 887
                     G+ V  K Q  T              +     KKS+  +R YS        
Sbjct: 281  SLEKCLGFTGENVQSKSQTSTSEEKSGALKGRTKKELAALRKKSYHMDRNYSAYGKRGGF 340

Query: 888  --------GRMSKKALK------------ANLTSWKKLNLSIEKVQSACESSSSVNNKN- 1004
                    G + +K LK            A+  S  K   +I        +S+ +++ + 
Sbjct: 341  KSGKVTIGGFVLEKRLKPPSEIPGEQATVASSKSSAKAGATIPSSDGRHHASTKISSASL 400

Query: 1005 ------PLPLK---------------ETNSEPVSQEPLKTAEY-YLSGIPFDE-AKGEHV 1115
                   LP+                E  S P ++ P     +   +G+P D+ + G+ +
Sbjct: 401  ATGGTVTLPISYLQPABRKLSQKSCLEKKSGPKAKTPASPKNHDCCAGLPCDDKSLGKDI 460

Query: 1116 AQDDKDEMILTATSLLQSL--------------------------------------QKE 1181
             +D+ DE+ L A   L+ L                                      Q +
Sbjct: 461  PRDEIDELFLKAVPQLKDLVPLDYCAGIPYDKSLGKYVVQDEKDELILNLVPRLEELQNK 520

Query: 1182 LKDWDDWANVKVMQVAKRLSYDRP-----------XXXXXXXXXXXXXXXXXXXSETTAA 1328
            L+ W +WAN KVMQV+ RL  +RP                              SE   A
Sbjct: 521  LESWTNWANEKVMQVSHRLGKERPELKILRQEKAEAEKFKKEKQMLEENTVKRISELENA 580

Query: 1329 LNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCESF 1508
            LN              RLE ENS LK++   A+  ++      +EA+++EQ+A+++ + +
Sbjct: 581  LNNATDQVEKATSKFCRLELENSTLKEELSAAKRMAIESVARNQEAIEREQKALKRAQVW 640

Query: 1509 GPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSER 1688
                             V   Q+DL KAK +  + E R  +EK E  K   +A+SIR ER
Sbjct: 641  EGLRSALQAELETGKKKVAQLQQDLGKAKNVHHQIEARWKREKIEKEKILAQADSIRKER 700

Query: 1689 EHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKKIAAMRKEMSWQPA 1868
            E  EAL K EE      AE +++EY  +I++++ ++  +KL++++ +IAA+R+  +    
Sbjct: 701  EQREALTKLEEDGIKLSAEYDMQEYMEDIRKVESKLSELKLKSDSSRIAALRRGAA---G 757

Query: 1869 ELTSSQSEVKHG--TKSN---------------KKDRECVMCLTEEMTVVFVPCGHQVLC 1997
                S S+ K G  TK N               ++DRECVMCL+EEM+VVF+PC HQV+C
Sbjct: 758  SFGGSPSDRKRGLATKGNQNATSSKKVRNSPDLRQDRECVMCLSEEMSVVFLPCAHQVVC 817

Query: 1998 AECNVLHEKSGMKDCPSCRTQIQKRITARFA 2090
            A CN LH+K GMKDCPSCRT IQ+RI  + A
Sbjct: 818  ANCNELHKKQGMKDCPSCRTPIQRRINVQHA 848


>ref|XP_013469244.1| E3 ubiquitin-protein ligase RF298-like protein, putative [Medicago
            truncatula]
 gb|KEH43282.1| E3 ubiquitin-protein ligase RF298-like protein, putative [Medicago
            truncatula]
          Length = 741

 Score =  262 bits (670), Expect = 8e-73
 Identities = 194/664 (29%), Positives = 313/664 (47%), Gaps = 57/664 (8%)
 Frame = +3

Query: 267  DWDSTTVSDLAKLLSDSLHTCLDTAIKQIV-ANGYSKEVAEQTVLKCGPFYSAKDMVSSI 443
            DW     + L  LL  +L      AIK++V   GYS+E+AE  V +    Y+  D +++I
Sbjct: 84   DWKDPMATQLEDLLMSNLEAIFSNAIKKVVDLGGYSQEMAEMAVSRKS-LYTEGDPLTNI 142

Query: 444  TDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICDL 623
                L+ LK +       + F +   L+ + ++EM+++LR +KPSL+V EAMW LL+ DL
Sbjct: 143  VYNTLNTLKGKGTETPADFVFQNTKQLLHYSLVEMLSVLRELKPSLTVTEAMWELLVHDL 202

Query: 624  NIIDASQADLDPQKFSIGGLKSGQGVGPSAEPESLPEPKTEPEPNTVPEPKTEPESKSAG 803
            +I      +        G L  G     S++  S    K   + + +        +  + 
Sbjct: 203  SITRVIAPE--------GQLSGGDSSEQSSDKSSTAPSKLNVQSDMIGSMSVTTFTNISQ 254

Query: 804  KKVDEKCQCCTKCCTGHKK--SFRFERTYSGRMSKKALKANLTSWKKLNLS---IEK--- 959
            +   E      K    HK+  S   E+ +  +  K   KA +   K  ++S   +EK   
Sbjct: 255  ESKSESNSQLMKSRKHHKEFTSVLREKFFHMKEPKACGKAGVKLGKLTSVSGLIVEKKLK 314

Query: 960  ---------------------VQSACESSSSVNNKNP-------LPLKE-TNSEPVSQ-- 1046
                                 +   C  S++ ++  P       LP K+ T++ P  +  
Sbjct: 315  PPSEVPNQKMKRGSSNTKGVSIAGVCRISTNDDSALPEGGSAVKLPTKDATSTSPTVKTA 374

Query: 1047 ----EPLKTA----EYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDW 1202
                +P  +A    + Y + IPFDEA G+ V +D+KDE +L   S  Q LQ E++ W+DW
Sbjct: 375  KPKPKPFSSAAQKVQNYCAAIPFDEASGKFVPRDEKDEQVLKLVSRAQELQDEVQSWNDW 434

Query: 1203 ANVKVMQVAKRL--------SYDRPXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXX 1358
            AN KVMQVA +L        S  +                    SE   A+         
Sbjct: 435  ANKKVMQVADKLRKLQVESKSLKKETEVYKKERKSLEENAEKRISEVENAMENNQKQLES 494

Query: 1359 XXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCESFGPXXXXXXXX 1538
                   LE + S+L+++   A++ +     S ++AL++EQ  +++ +S+          
Sbjct: 495  ATSSILILEAKKSLLEKELVSAKSLAEKSMASYQQALEREQTTIQQAQSWKTELDLLQDE 554

Query: 1539 XXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAE 1718
                   +    +++EK K LL  TE R+ KE+ +T K   +A   R ER+  EAL KAE
Sbjct: 555  LEKEKQKLANLHQEIEKGKNLLANTEGRVKKERAKTEKILAQAAYFRKERQQSEALMKAE 614

Query: 1719 EQITAEKAENELKEYESEIKRLQREIDAIKLEAETK-KIAAMRKEMSWQPAELTSSQSEV 1895
            E    +KA ++L+EY   + +L++EI  ++L++ +K   +++ KE       +T    + 
Sbjct: 615  EDAVRKKAASQLQEYVDSMVKLEKEIAVLRLKSGSKISTSSVVKENKKSETVMTGGSQDK 674

Query: 1896 KHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRI 2075
              G +  K++ ECVMCL+EE +VVF+PC HQVLC  CN LHEK GMK+CPSCRT I+ RI
Sbjct: 675  PAGGRL-KREHECVMCLSEERSVVFLPCAHQVLCPNCNELHEKQGMKECPSCRTTIEFRI 733

Query: 2076 TARF 2087
             A+F
Sbjct: 734  HAKF 737


>ref|XP_003591611.2| E3 ubiquitin-protein ligase RF298-like protein, putative [Medicago
            truncatula]
 gb|AES61862.2| E3 ubiquitin-protein ligase RF298-like protein, putative [Medicago
            truncatula]
          Length = 766

 Score =  262 bits (670), Expect = 1e-72
 Identities = 194/664 (29%), Positives = 313/664 (47%), Gaps = 57/664 (8%)
 Frame = +3

Query: 267  DWDSTTVSDLAKLLSDSLHTCLDTAIKQIV-ANGYSKEVAEQTVLKCGPFYSAKDMVSSI 443
            DW     + L  LL  +L      AIK++V   GYS+E+AE  V +    Y+  D +++I
Sbjct: 109  DWKDPMATQLEDLLMSNLEAIFSNAIKKVVDLGGYSQEMAEMAVSRKS-LYTEGDPLTNI 167

Query: 444  TDCALDYLKKETRNDATWYKFDDFDSLVKFMMLEMVTMLRHVKPSLSVREAMWTLLICDL 623
                L+ LK +       + F +   L+ + ++EM+++LR +KPSL+V EAMW LL+ DL
Sbjct: 168  VYNTLNTLKGKGTETPADFVFQNTKQLLHYSLVEMLSVLRELKPSLTVTEAMWELLVHDL 227

Query: 624  NIIDASQADLDPQKFSIGGLKSGQGVGPSAEPESLPEPKTEPEPNTVPEPKTEPESKSAG 803
            +I      +        G L  G     S++  S    K   + + +        +  + 
Sbjct: 228  SITRVIAPE--------GQLSGGDSSEQSSDKSSTAPSKLNVQSDMIGSMSVTTFTNISQ 279

Query: 804  KKVDEKCQCCTKCCTGHKK--SFRFERTYSGRMSKKALKANLTSWKKLNLS---IEK--- 959
            +   E      K    HK+  S   E+ +  +  K   KA +   K  ++S   +EK   
Sbjct: 280  ESKSESNSQLMKSRKHHKEFTSVLREKFFHMKEPKACGKAGVKLGKLTSVSGLIVEKKLK 339

Query: 960  ---------------------VQSACESSSSVNNKNP-------LPLKE-TNSEPVSQ-- 1046
                                 +   C  S++ ++  P       LP K+ T++ P  +  
Sbjct: 340  PPSEVPNQKMKRGSSNTKGVSIAGVCRISTNDDSALPEGGSAVKLPTKDATSTSPTVKTA 399

Query: 1047 ----EPLKTA----EYYLSGIPFDEAKGEHVAQDDKDEMILTATSLLQSLQKELKDWDDW 1202
                +P  +A    + Y + IPFDEA G+ V +D+KDE +L   S  Q LQ E++ W+DW
Sbjct: 400  KPKPKPFSSAAQKVQNYCAAIPFDEASGKFVPRDEKDEQVLKLVSRAQELQDEVQSWNDW 459

Query: 1203 ANVKVMQVAKRL--------SYDRPXXXXXXXXXXXXXXXXXXXSETTAALNTXXXXXXX 1358
            AN KVMQVA +L        S  +                    SE   A+         
Sbjct: 460  ANKKVMQVADKLRKLQVESKSLKKETEVYKKERKSLEENAEKRISEVENAMENNQKQLES 519

Query: 1359 XXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEALQKEQEAMRKCESFGPXXXXXXXX 1538
                   LE + S+L+++   A++ +     S ++AL++EQ  +++ +S+          
Sbjct: 520  ATSSILILEAKKSLLEKELVSAKSLAEKSMASYQQALEREQTTIQQAQSWKTELDLLQDE 579

Query: 1539 XXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETAKFRDRAESIRSEREHLEALAKAE 1718
                   +    +++EK K LL  TE R+ KE+ +T K   +A   R ER+  EAL KAE
Sbjct: 580  LEKEKQKLANLHQEIEKGKNLLANTEGRVKKERAKTEKILAQAAYFRKERQQSEALMKAE 639

Query: 1719 EQITAEKAENELKEYESEIKRLQREIDAIKLEAETK-KIAAMRKEMSWQPAELTSSQSEV 1895
            E    +KA ++L+EY   + +L++EI  ++L++ +K   +++ KE       +T    + 
Sbjct: 640  EDAVRKKAASQLQEYVDSMVKLEKEIAVLRLKSGSKISTSSVVKENKKSETVMTGGSQDK 699

Query: 1896 KHGTKSNKKDRECVMCLTEEMTVVFVPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRI 2075
              G +  K++ ECVMCL+EE +VVF+PC HQVLC  CN LHEK GMK+CPSCRT I+ RI
Sbjct: 700  PAGGRL-KREHECVMCLSEERSVVFLPCAHQVLCPNCNELHEKQGMKECPSCRTTIEFRI 758

Query: 2076 TARF 2087
             A+F
Sbjct: 759  HAKF 762


>ref|XP_010430601.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298 isoform X1
            [Camelina sativa]
          Length = 820

 Score =  259 bits (661), Expect = 6e-71
 Identities = 212/761 (27%), Positives = 328/761 (43%), Gaps = 124/761 (16%)
 Frame = +3

Query: 183  LKKEKYMAEMKSTLDLPKEDMEALAHDSDWDSTTVSDLAKLLSDSLHTCLDTAIKQIVAN 362
            LK +  + E  S   L  E+ E+L     WD      L +LLS +LH     A+ QI+  
Sbjct: 65   LKSQNSLCENDSNGQLKVEESESLG----WDDPFACQLEQLLSSNLHNLFRNAMNQIMDC 120

Query: 363  GYSKEVAEQTVLKCGPFYSAKDMVSSITDCALDYLKKETRNDATW-YKFDDFDSLVKFMM 539
            GYS++V  + +     +    D+VS+I +  L +LK   +   +  Y F+D   LV + +
Sbjct: 121  GYSEDVVLKAISSSRLYCGGNDLVSNIVNDTLSFLKSGKKVAGSRDYVFEDLQQLVAYTL 180

Query: 540  LEMVTMLRHVKPSLSVREAMWTLLICDLNIIDASQADLDPQKFSIGGLKSGQGVGPSAEP 719
            +E ++++R V+PSLS  EAMW LL+CDLN++ A + + +  + S G   +      +   
Sbjct: 181  VEKISLVREVRPSLSTVEAMWRLLMCDLNVLQAFEVEGEGFEGSSG--SNANKTSETQGA 238

Query: 720  ESLPEPKTEPEPNTVPEPKTE--------------------PESK---SAGKKV------ 812
            E +P   T+ + +  P   T+                    P +    + GK+V      
Sbjct: 239  EGIPPKSTDSDNSKSPVSNTQCSHSDPVKFGNFPNVNNSKNPHASGGATPGKEVFSVTTA 298

Query: 813  --------------DEKCQCCTKCCT-------GHKKSFRFERTYS-GRMSKKALKANLT 926
                          DEK   C K  T         K      RTYS G   K A      
Sbjct: 299  SAEGTKSASLTSVSDEKLVSCRKGRTKKELAMLRQKSCVEKIRTYSKGGGYKTAKFGGFL 358

Query: 927  SWKKLNLSIEKVQSACESSSS---VNNKNPLPLKE--------TNSEPVSQEPLKTAEYY 1073
              K+   + + + +   +SSS         +PL E        T++   S  P    + Y
Sbjct: 359  VEKRSKAASDLLSAQARNSSSKITTTEVVKIPLAESSGSSTLSTSNSTKSDTPALDVKEY 418

Query: 1074 LSGIPFDEA----KGEHVAQDDKDEMILTAT--------------------------SLL 1163
            ++ +P + A      E  +    +E    +T                           L+
Sbjct: 419  VTALPANNAPAIVASEKKSSSQPEEKASVSTKPAPDYYAAIPYDASLGIYVPRNKRDELI 478

Query: 1164 QSLQKELKD-------WDDWANVKVMQVAKRLSYDRP-----------XXXXXXXXXXXX 1289
              L   +KD       W DWAN KV Q   RL  D+P                       
Sbjct: 479  LKLVPRMKDLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLE 538

Query: 1290 XXXXXXXSETTAALNTXXXXXXXXXXXXFRLEYENSVLKQDREKAENRSLTEAQSLKEAL 1469
                   SE   ALN              RLE E S+LK++R+ A  R+   A+S +EA 
Sbjct: 539  ENTMKRRSEMELALNNATNQIERANNTVRRLELEQSLLKRERDAANIRAAESAESCREAK 598

Query: 1470 QKEQEAMRKCESFGPXXXXXXXXXXXXXXXVVPKQRDLEKAKGLLTETEIRLAKEKKETA 1649
            ++ Q +++  +S+                 V   Q+++ KAK    + E    +EK    
Sbjct: 599  ERVQRSLKNAQSWEGQKVLLQEELKSQRNKVAELQQEVAKAKTRQNQIEATWKQEKAAKG 658

Query: 1650 KFRDRAESIRSEREHLEALAKAEEQITAEKAENELKEYESEIKRLQREIDAIKLEAETKK 1829
            K   +A +++ ER  LE   KAEE+    KAEN++K Y   IKRL+ EI  ++L+++  K
Sbjct: 659  KLAAQAAALKKERVKLEEFGKAEEERIKTKAENDVKYYIENIKRLESEISKLELKSDGLK 718

Query: 1830 IAAMRKEMSWQPAELTSSQS------------EVKHGTKSN-KKDRECVMCLTEEMTVVF 1970
            IAA++K +    + +  + +            E  HG +S  K++RECVMCL EEM+V+F
Sbjct: 719  IAALKKGIDGNKSGMNHTTTTKANTMAGAKVWENNHGEESKIKRERECVMCLCEEMSVIF 778

Query: 1971 VPCGHQVLCAECNVLHEKSGMKDCPSCRTQIQKRITARFAK 2093
            +PC HQVLC++CN LHEK  M+DCPSCR +IQ+RI ARFA+
Sbjct: 779  LPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 819