BLASTX nr result
ID: Chrysanthemum21_contig00022497
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Chrysanthemum21_contig00022497
(1665 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_021982081.1| cytoplasmic tRNA 2-thiolation protein 2 isof... 541 0.0
ref|XP_021982080.1| cytoplasmic tRNA 2-thiolation protein 2 isof... 536 0.0
ref|XP_023737631.1| cytoplasmic tRNA 2-thiolation protein 2 [Lac... 531 0.0
gb|KVI12121.1| Thiouridylase, cytoplasmic, subunit 2 [Cynara car... 484 e-166
ref|XP_017238071.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 436 e-145
ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 434 e-144
ref|XP_012491368.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 432 e-144
ref|XP_015898174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 433 e-143
ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 431 e-143
ref|XP_018805161.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 431 e-143
gb|EOY09232.1| Adenine nucleotide alpha hydrolases-like superfam... 431 e-143
gb|PPD68667.1| hypothetical protein GOBAR_DD34458 [Gossypium bar... 429 e-143
ref|XP_016696761.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 431 e-143
ref|XP_018841765.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 430 e-143
ref|XP_021282370.1| cytoplasmic tRNA 2-thiolation protein 2 [Her... 430 e-143
ref|XP_012491365.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 429 e-143
ref|XP_019224820.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 429 e-142
ref|XP_015089096.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 429 e-142
ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro... 429 e-142
ref|XP_022766665.1| cytoplasmic tRNA 2-thiolation protein 2 isof... 428 e-142
>ref|XP_021982081.1| cytoplasmic tRNA 2-thiolation protein 2 isoform X2 [Helianthus
annuus]
gb|OTG14720.1| putative adenine nucleotide alpha hydrolases-like superfamily protein
[Helianthus annuus]
Length = 455
Score = 541 bits (1394), Expect(2) = 0.0
Identities = 263/307 (85%), Positives = 290/307 (94%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
CLKCK++EAT+FTGG+GGGRFCADCFRSNLYGKFKLAVTSNAMISP DKVLVAFSGG SS
Sbjct: 44 CLKCKATEATVFTGGDGGGRFCADCFRSNLYGKFKLAVTSNAMISPTDKVLVAFSGGPSS 103
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RVALQFVSEMQLKAQKN+DATANRDRSLPVF VGVAFIDESE+HP+AS DKA+EDIK
Sbjct: 104 RVALQFVSEMQLKAQKNYDATANRDRSLPVFAVGVAFIDESEIHPVASAAFDKAVEDIKE 163
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NL PTKELHV PI +YSTKT+DGK+ML+NL N+V+DATGKEDLLTHLRM+SLQK+
Sbjct: 164 IVSNLPLPTKELHVAPISHVYSTKTSDGKQMLRNLTNSVSDATGKEDLLTHLRMLSLQKV 223
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A +NGYTKLVLGSCTSRIACHVL+ATVKGQGYSLAADIQYADARWEVPVVLPLRDC +QE
Sbjct: 224 AIKNGYTKLVLGSCTSRIACHVLSATVKGQGYSLAADIQYADARWEVPVVLPLRDCPLQE 283
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+NILCNLDSLKIV++FN++ AGINGL+SSFVK+LQEENP+RECTIVRTAGKLTPFPFNRI
Sbjct: 284 LNILCNLDSLKIVDIFNETGAGINGLVSSFVKILQEENPSRECTIVRTAGKLTPFPFNRI 343
Query: 1084 PEANVDD 1104
PEANVDD
Sbjct: 344 PEANVDD 350
Score = 154 bits (389), Expect(2) = 0.0
Identities = 70/88 (79%), Positives = 76/88 (86%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMSCYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESI 1300
ESLPPE+FCPICNSPMSCY N + +F A CC SCQYQILP+E SSMDQFYSFLPESI
Sbjct: 368 ESLPPESFCPICNSPMSCYGNAESRPELFRAACCPSCQYQILPDEASSMDQFYSFLPESI 427
Query: 1301 ISRAKNGNEKWIREKIQDCLLSDGEDEV 1384
I+RAKNGN+KWIREKIQDCLL D EDEV
Sbjct: 428 IARAKNGNQKWIREKIQDCLLLDDEDEV 455
>ref|XP_021982080.1| cytoplasmic tRNA 2-thiolation protein 2 isoform X1 [Helianthus
annuus]
Length = 456
Score = 536 bits (1382), Expect(2) = 0.0
Identities = 263/308 (85%), Positives = 290/308 (94%), Gaps = 1/308 (0%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
CLKCK++EAT+FTGG+GGGRFCADCFRSNLYGKFKLAVTSNAMISP DKVLVAFSGG SS
Sbjct: 44 CLKCKATEATVFTGGDGGGRFCADCFRSNLYGKFKLAVTSNAMISPTDKVLVAFSGGPSS 103
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RVALQFVSEMQLKAQKN+DATANRDRSLPVF VGVAFIDESE+HP+AS DKA+EDIK
Sbjct: 104 RVALQFVSEMQLKAQKNYDATANRDRSLPVFAVGVAFIDESEIHPVASAAFDKAVEDIKE 163
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NL PTKELHV PI +YSTKT+DGK+ML+NL N+V+DATGKEDLLTHLRM+SLQK+
Sbjct: 164 IVSNLPLPTKELHVAPISHVYSTKTSDGKQMLRNLTNSVSDATGKEDLLTHLRMLSLQKV 223
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A +NGYTKLVLGSCTSRIACHVL+ATVKGQGYSLAADIQYADARWEVPVVLPLRDC +QE
Sbjct: 224 AIKNGYTKLVLGSCTSRIACHVLSATVKGQGYSLAADIQYADARWEVPVVLPLRDCPLQE 283
Query: 904 INILCNLD-SLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNR 1080
+NILCNLD SLKIV++FN++ AGINGL+SSFVK+LQEENP+RECTIVRTAGKLTPFPFNR
Sbjct: 284 LNILCNLDSSLKIVDIFNETGAGINGLVSSFVKILQEENPSRECTIVRTAGKLTPFPFNR 343
Query: 1081 IPEANVDD 1104
IPEANVDD
Sbjct: 344 IPEANVDD 351
Score = 154 bits (389), Expect(2) = 0.0
Identities = 70/88 (79%), Positives = 76/88 (86%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMSCYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYSFLPESI 1300
ESLPPE+FCPICNSPMSCY N + +F A CC SCQYQILP+E SSMDQFYSFLPESI
Sbjct: 369 ESLPPESFCPICNSPMSCYGNAESRPELFRAACCPSCQYQILPDEASSMDQFYSFLPESI 428
Query: 1301 ISRAKNGNEKWIREKIQDCLLSDGEDEV 1384
I+RAKNGN+KWIREKIQDCLL D EDEV
Sbjct: 429 IARAKNGNQKWIREKIQDCLLLDDEDEV 456
>ref|XP_023737631.1| cytoplasmic tRNA 2-thiolation protein 2 [Lactuca sativa]
gb|PLY70842.1| hypothetical protein LSAT_8X81281 [Lactuca sativa]
Length = 465
Score = 531 bits (1367), Expect = 0.0
Identities = 262/306 (85%), Positives = 286/306 (93%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
CLKCK++EATIFTGGEGG RFCADCFRSNLYGKFKLAVTS+AMISP DKVLVAFSGG SS
Sbjct: 46 CLKCKAAEATIFTGGEGGSRFCADCFRSNLYGKFKLAVTSHAMISPTDKVLVAFSGGPSS 105
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RVALQFVSEMQLK+QKNFDATA RDRSLPVFGVGVAFIDE+E+HPI D +KAIEDIK
Sbjct: 106 RVALQFVSEMQLKSQKNFDATAIRDRSLPVFGVGVAFIDETELHPITCTDFNKAIEDIKE 165
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NL+P KELH+VPIR+IYSTKT+DG E L+NLINTV+DATGKEDLL+HLRM+SLQKI
Sbjct: 166 IVSNLSPTKKELHIVPIRNIYSTKTSDGSETLKNLINTVSDATGKEDLLSHLRMLSLQKI 225
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A ENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWE+PVVLPLRDCL+QE
Sbjct: 226 AIENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLIQE 285
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+NILC+LDSLKIVE F DS+ GINGL+SSFVK+LQEENP+RECTIVRTAGKLTPFPFN+I
Sbjct: 286 LNILCSLDSLKIVEEFKDSRGGINGLVSSFVKLLQEENPSRECTIVRTAGKLTPFPFNKI 345
Query: 1084 PEANVD 1101
PE N D
Sbjct: 346 PETNTD 351
Score = 139 bits (351), Expect = 3e-32
Identities = 64/94 (68%), Positives = 76/94 (80%), Gaps = 6/94 (6%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPM------SCYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYS 1282
ESLPP++FCPICNSP+ + + N Q +FGA CCSSCQYQILPEE SSM++FYS
Sbjct: 372 ESLPPDSFCPICNSPIRDLQSVTSFTNSQTTPELFGAACCSSCQYQILPEELSSMEEFYS 431
Query: 1283 FLPESIISRAKNGNEKWIREKIQDCLLSDGEDEV 1384
FLP+SII RA+NGN+KWIREKIQDCL+ D EDEV
Sbjct: 432 FLPQSIIERARNGNQKWIREKIQDCLILDDEDEV 465
>gb|KVI12121.1| Thiouridylase, cytoplasmic, subunit 2 [Cynara cardunculus var.
scolymus]
Length = 447
Score = 484 bits (1245), Expect(2) = e-166
Identities = 250/330 (75%), Positives = 269/330 (81%), Gaps = 23/330 (6%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
CLKCK +EATIFTGG+GGGRFCADCFRSNLYGKFKLAVTSNAMISP DKVLVAFSGG SS
Sbjct: 43 CLKCKGTEATIFTGGDGGGRFCADCFRSNLYGKFKLAVTSNAMISPTDKVLVAFSGGPSS 102
Query: 364 R-----------------------VALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAF 474
VALQFVSEMQLKAQKNFDATA+RDRSLPVFGVGVAF
Sbjct: 103 SCSDLSQANSLDQVLLDTFKDLNVVALQFVSEMQLKAQKNFDATADRDRSLPVFGVGVAF 162
Query: 475 IDESEVHPIASGDLDKAIEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLIN 654
IDESE+HP + DLDKA+ED+K IVLNLAPP KELHVVPIRSI S KT+DG+E L+NL+N
Sbjct: 163 IDESEIHPSTAADLDKAVEDMKEIVLNLAPPRKELHVVPIRSICSEKTSDGRETLENLVN 222
Query: 655 TVTDATGKEDLLTHLRMMSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAAD 834
IA ENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAAD
Sbjct: 223 A---------------------IAIENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAAD 261
Query: 835 IQYADARWEVPVVLPLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEE 1014
IQYADARWE+PVVLPLRDCLMQE+NILC+LDSLKIVEVFNDS+A INGL+SSFVK+LQEE
Sbjct: 262 IQYADARWEIPVVLPLRDCLMQELNILCSLDSLKIVEVFNDSRANINGLVSSFVKLLQEE 321
Query: 1015 NPARECTIVRTAGKLTPFPFNRIPEANVDD 1104
NP+RECTIVRTAGKLTPFPFNRIPE + DD
Sbjct: 322 NPSRECTIVRTAGKLTPFPFNRIPEVDADD 351
Score = 130 bits (328), Expect(2) = e-166
Identities = 59/79 (74%), Positives = 66/79 (83%), Gaps = 6/79 (7%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPM------SCYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFYS 1282
ESLPPE+FCPICNSPM SCY NPQP +F A CCS CQYQ+LPEEPSSMDQFYS
Sbjct: 369 ESLPPESFCPICNSPMNDLQNSSCYSNPQPNPELFRAACCSCCQYQVLPEEPSSMDQFYS 428
Query: 1283 FLPESIISRAKNGNEKWIR 1339
FLP+SII+RAK+GN+KWIR
Sbjct: 429 FLPKSIITRAKDGNQKWIR 447
>ref|XP_017238071.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Daucus carota
subsp. sativus]
Length = 460
Score = 436 bits (1120), Expect = e-145
Identities = 229/336 (68%), Positives = 267/336 (79%), Gaps = 5/336 (1%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEG--GG---RFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFS 348
CLKCKSSE I GG G GG + CADCFR NLYGKFKLAV++N MISP D VLVAFS
Sbjct: 37 CLKCKSSE--IIAGGTGFSGGTNMKLCADCFRGNLYGKFKLAVSANGMISPSDNVLVAFS 94
Query: 349 GGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAI 528
GG SSRVALQFV EMQ+K+QKNFDA+ RDRSLPVFGVGVAF+DES H + S +LDK I
Sbjct: 95 GGTSSRVALQFVHEMQVKSQKNFDAS--RDRSLPVFGVGVAFVDESAFHTLPSHELDKEI 152
Query: 529 EDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMM 708
E+IK IV L+PPTK HV+PI SI + +N K+ L L+N V+D TGKEDL+ LRMM
Sbjct: 153 EEIKLIVDELSPPTKAFHVIPIESICLSNSNSAKDNLNELLNAVSDKTGKEDLMVQLRMM 212
Query: 709 SLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRD 888
SLQK A ENGYTKLVLGSCTSR+ACHV+A+TVKGQGYSLAADIQY D+RWE+PVVLP+RD
Sbjct: 213 SLQKTALENGYTKLVLGSCTSRLACHVIASTVKGQGYSLAADIQYVDSRWEIPVVLPIRD 272
Query: 889 CLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPF 1068
C +QE+N+LC L+SLK +E+ N S+AGINGLISSFVK+LQEENP+RECTIVRTAGKLTPF
Sbjct: 273 CTLQELNMLCKLESLKTMELHNGSRAGINGLISSFVKLLQEENPSRECTIVRTAGKLTPF 332
Query: 1069 PFNRIPEANVDDXXXXXXISSPRGILSYLQQPNELL 1176
FN+IPE DD ++S R + + NELL
Sbjct: 333 HFNKIPE-EADD--CNLQLASQRRRKKFNLKSNELL 365
Score = 100 bits (248), Expect = 1e-18
Identities = 49/96 (51%), Positives = 65/96 (67%), Gaps = 10/96 (10%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMS-------CYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQFY 1279
E LPPE++CP+C+SP+ ++N F CCSSCQ+QILP+EP M+QFY
Sbjct: 363 ELLPPESYCPLCSSPLDKNSISNLAFENGHTSYGSFVPKCCSSCQFQILPKEPLHMEQFY 422
Query: 1280 SFLPESIISRAKNG---NEKWIREKIQDCLLSDGED 1378
S LP+ I RAK+G +++ IRE+IQDCLLSD ED
Sbjct: 423 SLLPQPITDRAKDGSCRSQRSIREQIQDCLLSDNED 458
>ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
tuberosum]
Length = 481
Score = 434 bits (1117), Expect = e-144
Identities = 219/313 (69%), Positives = 256/313 (81%), Gaps = 9/313 (2%)
Frame = +1
Query: 184 CLKCK---------SSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVL 336
CLKCK ++E GG GGRFCADCFRSNL+GKF+LAVT+NAMISP D VL
Sbjct: 52 CLKCKVNRTIAAAAAAEGGGDFGGGDGGRFCADCFRSNLFGKFRLAVTANAMISPSDNVL 111
Query: 337 VAFSGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDL 516
VAFSGG SRVALQF EMQ KAQKN+DA+ RDR+LPVFGVGVAFIDE + ++S +L
Sbjct: 112 VAFSGGTCSRVALQFTHEMQSKAQKNYDAS--RDRALPVFGVGVAFIDEKAISAVSSDNL 169
Query: 517 DKAIEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTH 696
D+A+E++K IV +LAPP K+ HVVP +IYS K D K+ L LINTV D TGKEDLL H
Sbjct: 170 DRAVEEMKLIVSDLAPPVKQFHVVPTEAIYSLKAGDAKDRLNELINTVNDVTGKEDLLEH 229
Query: 697 LRMMSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVL 876
RM+SLQKIA ENGYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVL
Sbjct: 230 FRMLSLQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVL 289
Query: 877 PLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGK 1056
PLRDC + E+N+LC+LDSLK VEVFN ++AGINGLISSFVK+LQEENP+RE TI+RTAGK
Sbjct: 290 PLRDCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSFVKLLQEENPSRESTIMRTAGK 349
Query: 1057 LTPFPFNRIPEAN 1095
LTPF FNR+PE N
Sbjct: 350 LTPFHFNRMPEDN 362
Score = 100 bits (250), Expect = 6e-19
Identities = 46/97 (47%), Positives = 68/97 (70%), Gaps = 11/97 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMSC--------YKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQF 1276
++LPPE+FCPICNSP+ + N + N GA CC+SCQ+Q+LP +PSS++ F
Sbjct: 383 DALPPESFCPICNSPLKISNFGTSVTFDNGRTNPNGIGAACCASCQFQVLPNDPSSLEHF 442
Query: 1277 YSFLPESIISRAKNG---NEKWIREKIQDCLLSDGED 1378
S LP S++S A++G +++ +RE+I+DCLLSD ED
Sbjct: 443 ISLLPPSMVSEAEDGDRLSQRQLREQIEDCLLSDNED 479
>ref|XP_012491368.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X2
[Gossypium raimondii]
gb|KJB43134.1| hypothetical protein B456_007G186200 [Gossypium raimondii]
gb|KJB43137.1| hypothetical protein B456_007G186200 [Gossypium raimondii]
Length = 460
Score = 432 bits (1112), Expect = e-144
Identities = 216/302 (71%), Positives = 252/302 (83%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCK +E G RFC DCF++NLYGKFK AVT NAMI+P DKVLVAFSGG SS
Sbjct: 41 CVKCKVNEPVCCGIGGENSRFCKDCFKNNLYGKFKQAVTYNAMITPPDKVLVAFSGGPSS 100
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ KAQKN+DA N+D+SLPVFGVGVAFIDES H S ++KAIEDI+
Sbjct: 101 RVVLQFVHEMQYKAQKNYDA--NKDKSLPVFGVGVAFIDESSTHSFTSQHIEKAIEDIRL 158
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NLAPP+KEL VVPI SI+S+ DGKE L+ L++ V+D TGKEDLL+HLR++ LQKI
Sbjct: 159 IVSNLAPPSKELFVVPIESIFSSDGIDGKERLKELLDAVSDVTGKEDLLSHLRILLLQKI 218
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGYT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RWE+PVVLPLRDC QE
Sbjct: 219 ASENGYTRIVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCPAQE 278
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC+LD LKIVE+ N +GINGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNR+
Sbjct: 279 LNTLCSLDGLKIVELLNGPCSGINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRV 338
Query: 1084 PE 1089
PE
Sbjct: 339 PE 340
Score = 85.9 bits (211), Expect = 4e-14
Identities = 45/98 (45%), Positives = 66/98 (67%), Gaps = 11/98 (11%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPMSCYKNPQPL--------DNIFGATCCSSCQYQILPEEPSSMDQFY 1279
SL ++FCPICNSP+ P L ++F A+ CSSCQ+QILP++PS M++F
Sbjct: 364 SLSSDSFCPICNSPLKKSNFPSSLRSHGSQQNPDLF-ASACSSCQFQILPKDPSLMEEFL 422
Query: 1280 SFLPESIISRAKN---GNEKWIREKIQDCLLSDGEDEV 1384
S LP+ +I++AK+ GN +RE+IQ+ L SDGE+E+
Sbjct: 423 SLLPQQMITKAKHGDQGNFSLLREQIQEFLFSDGENEI 460
>ref|XP_015898174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X2
[Ziziphus jujuba]
Length = 505
Score = 433 bits (1113), Expect = e-143
Identities = 217/306 (70%), Positives = 253/306 (82%), Gaps = 2/306 (0%)
Frame = +1
Query: 184 CLKCKSSEATIFTGG--EGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGA 357
C+KCK+++ F G + RFC DCFRSNL+GKF+LAVTSNAMI P D VLVAFSGGA
Sbjct: 83 CIKCKANQPMSFAGRRTDDDARFCGDCFRSNLFGKFRLAVTSNAMICPTDNVLVAFSGGA 142
Query: 358 SSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDI 537
SSRVALQFV EMQ KAQKNFDA+ RDRSLPVFGVGVAFIDES + + ++ K IEDI
Sbjct: 143 SSRVALQFVHEMQHKAQKNFDAS--RDRSLPVFGVGVAFIDESSIFTVPEDEVTKGIEDI 200
Query: 538 KGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQ 717
+ IV +L PPTKELHVVPI S+YS+ +NDG + L+ L++ V DATGKEDLL +LRM+SLQ
Sbjct: 201 RLIVSSLTPPTKELHVVPIESLYSSDSNDGSDRLKKLLDAVCDATGKEDLLVYLRMLSLQ 260
Query: 718 KIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLM 897
KIA+ENGY +LVLGSCTSRIACHVL ATVKG GYSL ADIQY D+RW VPVVLPLRDCL+
Sbjct: 261 KIASENGYNRLVLGSCTSRIACHVLTATVKGHGYSLPADIQYVDSRWGVPVVLPLRDCLI 320
Query: 898 QEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFN 1077
QE+N+LC+LD LK VE+ + + INGL+SSFV +LQEENP+RECTIVRTAGKLTPF FN
Sbjct: 321 QELNMLCHLDGLKTVELNKNPSSSINGLVSSFVTLLQEENPSRECTIVRTAGKLTPFHFN 380
Query: 1078 RIPEAN 1095
RIPE N
Sbjct: 381 RIPEIN 386
Score = 102 bits (255), Expect = 2e-19
Identities = 50/98 (51%), Positives = 69/98 (70%), Gaps = 11/98 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMS-----CYKNPQPLDN---IFGATCCSSCQYQILPEEPSSMDQF 1276
ES+ E+FCPICNSP+S +KN + D +F A CCSSC++QILP++PSS+ F
Sbjct: 407 ESISSESFCPICNSPLSKSDLLSFKNVESCDTNSVLFSAACCSSCRFQILPKDPSSVGDF 466
Query: 1277 YSFLPESIISRAKNG---NEKWIREKIQDCLLSDGEDE 1381
YS LP+ +++RAK G N +RE+I+DC+LSD EDE
Sbjct: 467 YSLLPQPVVARAKCGSADNYSLLREQIKDCILSDSEDE 504
>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
emb|CBI29838.3| unnamed protein product, partial [Vitis vinifera]
Length = 468
Score = 431 bits (1109), Expect = e-143
Identities = 228/333 (68%), Positives = 263/333 (78%), Gaps = 4/333 (1%)
Frame = +1
Query: 184 CLKCKSSEATIFTGG---EGG-GRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSG 351
CLKCK +E +G +G GRFC DCFR NL+GKFKLAVTSNAMISP D VLVAFSG
Sbjct: 47 CLKCKVNETLASSGTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSG 106
Query: 352 GASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIE 531
G SSRVALQFV EMQ KAQKNFDA+ RDRSLPVFGVGVAF+DES V I S + DKAI+
Sbjct: 107 GPSSRVALQFVHEMQHKAQKNFDAS--RDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQ 164
Query: 532 DIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMS 711
D+K IV NLAPP K HVVPI S+YS+ +G++ L+ L+N V D TGKEDLL HLRM+S
Sbjct: 165 DMKSIVSNLAPPDKAFHVVPIESVYSS---NGRDRLEELLNAVKDVTGKEDLLQHLRMLS 221
Query: 712 LQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDC 891
LQK A ENGYTKL+LGSCTSRIACHV++ATVKGQGYSL ADIQY DARWE+PVVLPLRDC
Sbjct: 222 LQKNAYENGYTKLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDC 281
Query: 892 LMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFP 1071
L QE+N+LC LD LK +EV N +GINGL++SFV +LQEENP+RECTIVRTAGKLTPF
Sbjct: 282 LAQELNMLCRLDGLKTMEVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFH 341
Query: 1072 FNRIPEANVDDXXXXXXISSPRGILSYLQQPNE 1170
FN+IPE+ DD ++S R + +PNE
Sbjct: 342 FNQIPES--DD--SNVHLASRRRQKKFNLKPNE 370
Score = 101 bits (251), Expect = 4e-19
Identities = 50/97 (51%), Positives = 68/97 (70%), Gaps = 11/97 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMS--------CYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQF 1276
ES+PPE++CPICNSP+S + Q +IFGATCC SCQ+QILP+EP SM+ F
Sbjct: 370 ESVPPESYCPICNSPLSKSDHINLSSLGSDQTNADIFGATCCPSCQFQILPKEPVSMEHF 429
Query: 1277 YSFLPESIISRAKNGN---EKWIREKIQDCLLSDGED 1378
YS LP+ + +AK+ + ++ +RE+IQD LLSD ED
Sbjct: 430 YSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDSED 466
>ref|XP_018805161.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Juglans
regia]
Length = 461
Score = 431 bits (1108), Expect = e-143
Identities = 219/331 (66%), Positives = 266/331 (80%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCKS+E I G GRFCADCFRSNLYGKF+LAV SNAMI P D VLVAFSGG+SS
Sbjct: 42 CVKCKSNEP-ISGGNAEDGRFCADCFRSNLYGKFRLAVISNAMICPTDNVLVAFSGGSSS 100
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RVALQFV EMQ K+QKNFDA+ RDRSLPVFGVGVAFIDE + P++S ++DKAI+D +
Sbjct: 101 RVALQFVHEMQDKSQKNFDAS--RDRSLPVFGVGVAFIDERAIFPVSSHEIDKAIQDFRS 158
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV LAPP KELHVVPI +I S+ ++DG++ L+NL++ ++D+TGKEDLL HLRM+SLQKI
Sbjct: 159 IVSALAPPRKELHVVPIENICSSSSSDGRDRLKNLLDAISDSTGKEDLLLHLRMLSLQKI 218
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGY ++VLGSCTS IACHV++A VKGQGYSL DIQY DARWE+PVVLPLRDCL QE
Sbjct: 219 ASENGYNRVVLGSCTSSIACHVISAIVKGQGYSLPGDIQYVDARWEIPVVLPLRDCLTQE 278
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC+LD LK VE+ +GINGL+SSFV +LQ+ENP+RECTIVRTAGKLTPF FN I
Sbjct: 279 LNRLCHLDGLKTVELHKVPYSGINGLVSSFVTLLQKENPSRECTIVRTAGKLTPFHFNSI 338
Query: 1084 PEANVDDXXXXXXISSPRGILSYLQQPNELL 1176
PE + + +++ R + Y +PNE L
Sbjct: 339 PEKSDSN----TTLATQRRMKKYNLKPNESL 365
Score = 108 bits (271), Expect = 1e-21
Identities = 54/110 (49%), Positives = 74/110 (67%), Gaps = 11/110 (10%)
Frame = +2
Query: 1085 QKQM*MTXXXXXESLPPEAFCPICNSPM--------SCYKNPQPLDNIFGATCCSSCQYQ 1240
Q++M ESL E+FCP+CNSP+ S +N Q +IFGA CCSSC++Q
Sbjct: 351 QRRMKKYNLKPNESLSSESFCPVCNSPLNKSDLLRQSNLQNHQTSSDIFGAACCSSCRFQ 410
Query: 1241 ILPEEPSSMDQFYSFLPESIISRAKN---GNEKWIREKIQDCLLSDGEDE 1381
ILP++PSSM+ FY+ LP+ +++RAK GN +RE+IQDCL+SD EDE
Sbjct: 411 ILPKDPSSMEHFYTHLPQPLVARAKRGGYGNLSLLREQIQDCLISDSEDE 460
>gb|EOY09232.1| Adenine nucleotide alpha hydrolases-like superfamily protein,
putative [Theobroma cacao]
Length = 464
Score = 431 bits (1108), Expect = e-143
Identities = 213/302 (70%), Positives = 253/302 (83%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCKS+E G RFC DCF++NLYGKFK AV+ NAMI+P DKVLVAFSGG SS
Sbjct: 43 CVKCKSNEPVCCGIGGDDARFCKDCFKTNLYGKFKQAVSYNAMITPSDKVLVAFSGGPSS 102
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ +AQKNFDA+ +DRSLPVFGVGVAFIDES +H AS D +KAIEDI+
Sbjct: 103 RVVLQFVHEMQCRAQKNFDAS--KDRSLPVFGVGVAFIDESSIHSFASQDAEKAIEDIRS 160
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NLAPP KEL+VVPI +I+S+ DGKE ++ L++ V+D TGKEDLL HLRM+SLQ++
Sbjct: 161 IVSNLAPPEKELYVVPIENIFSSDNIDGKERMKKLLDAVSDVTGKEDLLIHLRMLSLQRV 220
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A++N YT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RW+VPVVLPLRDC QE
Sbjct: 221 ASQNRYTRVVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWQVPVVLPLRDCPAQE 280
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC+LD LKIVE+ N + INGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 281 LNTLCSLDGLKIVELLNGPCSSINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRI 340
Query: 1084 PE 1089
P+
Sbjct: 341 PD 342
Score = 92.0 bits (227), Expect = 4e-16
Identities = 51/99 (51%), Positives = 69/99 (69%), Gaps = 12/99 (12%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPM------SCYKNPQPLDN--IFGATC-CSSCQYQILPEEPSSMDQF 1276
SL E+FCPICNSP+ S ++ Q +N IF A CSSCQ+QI+P+EPS M+QF
Sbjct: 366 SLSSESFCPICNSPLNKSNFSSSLRSLQSNENSDIFAAAAACSSCQFQIIPKEPSLMEQF 425
Query: 1277 YSFLPESIISRAKNGNE---KWIREKIQDCLLSDGEDEV 1384
S LP+ +I+RAK+G+ +RE+IQ+ LLSDGEDE+
Sbjct: 426 VSLLPQPLITRAKHGDRGDFSLLREQIQEFLLSDGEDEI 464
>gb|PPD68667.1| hypothetical protein GOBAR_DD34458 [Gossypium barbadense]
Length = 446
Score = 429 bits (1104), Expect = e-143
Identities = 217/303 (71%), Positives = 253/303 (83%), Gaps = 1/303 (0%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCK +E G RFC DCF++NLYGKFK AVT NAMI+P DKVLVAFSGG SS
Sbjct: 41 CVKCKVNEPVCCGIGGENSRFCKDCFKNNLYGKFKQAVTYNAMITPPDKVLVAFSGGPSS 100
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ KAQKN+DA N+D+SLPVFGVGVAFIDES H S ++KAIEDI+
Sbjct: 101 RVVLQFVHEMQYKAQKNYDA--NKDKSLPVFGVGVAFIDESSTHSFTSQHIEKAIEDIRL 158
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NLAPP+KEL VVPI SI+S+ DGKE L+ L++ V+D TGKEDLL+HLR++ LQKI
Sbjct: 159 IVSNLAPPSKELFVVPIESIFSSDGIDGKERLKELLDAVSDVTGKEDLLSHLRILLLQKI 218
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGYT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RWE+PVVLPLRDC QE
Sbjct: 219 ASENGYTRIVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCPAQE 278
Query: 904 INILCNLD-SLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNR 1080
+N LC+LD SLKIVE+ N +GINGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNR
Sbjct: 279 LNTLCSLDGSLKIVELLNGPCSGINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNR 338
Query: 1081 IPE 1089
+PE
Sbjct: 339 VPE 341
Score = 70.5 bits (171), Expect = 4e-09
Identities = 34/76 (44%), Positives = 52/76 (68%), Gaps = 8/76 (10%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPMSCYKNPQPL--------DNIFGATCCSSCQYQILPEEPSSMDQFY 1279
SL ++FCPICNSP+ P L ++F A+ CSSCQ+QILP++PS M++F
Sbjct: 365 SLSSDSFCPICNSPLKKSNFPSSLRSHGSQQNPDLF-ASACSSCQFQILPKDPSLMEEFL 423
Query: 1280 SFLPESIISRAKNGNE 1327
S LP+ +I++AK+G++
Sbjct: 424 SLLPQQMITKAKHGDQ 439
>ref|XP_016696761.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like isoform X2
[Gossypium hirsutum]
Length = 478
Score = 431 bits (1107), Expect = e-143
Identities = 215/302 (71%), Positives = 251/302 (83%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCK +E G RFC DCF++NLYGKFK AVT NAMI+P DKVLVAFSGG SS
Sbjct: 41 CVKCKVNEPVCCGIGGENSRFCKDCFKNNLYGKFKQAVTYNAMITPPDKVLVAFSGGPSS 100
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ KAQKN+DA N+D+SLPVFGVGVAFIDES H S ++KAIEDI+
Sbjct: 101 RVVLQFVHEMQYKAQKNYDA--NKDKSLPVFGVGVAFIDESSTHSFTSQHIEKAIEDIRL 158
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NLAPP+KEL VVPI I+S+ DGKE L+ L++ V+D TGKEDLL+HLR++ LQKI
Sbjct: 159 IVSNLAPPSKELFVVPIERIFSSDGIDGKERLKELLDAVSDVTGKEDLLSHLRILLLQKI 218
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGYT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RWE+PVVLPLRDC QE
Sbjct: 219 ASENGYTRIVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCPAQE 278
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC+LD LKIVE+ N +GINGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNR+
Sbjct: 279 LNTLCSLDGLKIVELLNGPCSGINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRV 338
Query: 1084 PE 1089
PE
Sbjct: 339 PE 340
Score = 70.5 bits (171), Expect = 4e-09
Identities = 34/76 (44%), Positives = 52/76 (68%), Gaps = 8/76 (10%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPMSCYKNPQPL--------DNIFGATCCSSCQYQILPEEPSSMDQFY 1279
SL ++FCPICNSP+ P L ++F A+ CSSCQ+QILP++PS M++F
Sbjct: 364 SLSSDSFCPICNSPLKKSNFPSSLRSHGSQQNPDLF-ASACSSCQFQILPKDPSLMEEFL 422
Query: 1280 SFLPESIISRAKNGNE 1327
S LP+ +I++AK+G++
Sbjct: 423 SLLPQQMITKAKHGDQ 438
>ref|XP_018841765.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Juglans
regia]
Length = 461
Score = 430 bits (1105), Expect = e-143
Identities = 219/331 (66%), Positives = 264/331 (79%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCKS+E I G GRFCADCFRSNLYGKF+LAV SNAMI P D VLVAFSGG SS
Sbjct: 42 CVKCKSNEP-ISGGNAEDGRFCADCFRSNLYGKFRLAVISNAMICPTDNVLVAFSGGPSS 100
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RVALQFV EMQ K+QKNFDA+ RDRSLPVFGVGVAFIDE + P+ S ++DKAI+D +
Sbjct: 101 RVALQFVHEMQDKSQKNFDAS--RDRSLPVFGVGVAFIDERAIFPVPSHEIDKAIQDFRS 158
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV LAPP KELHVVPI +I S+ ++DG++ L+NL++ ++D+TGKEDLL HLRM+SLQKI
Sbjct: 159 IVSALAPPRKELHVVPIENICSSSSSDGRDRLKNLLDAISDSTGKEDLLLHLRMLSLQKI 218
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGY ++VLGSCTS IACHV++A VKGQGYSL DIQY DARWE+PVVLPLRDCL QE
Sbjct: 219 ASENGYNRVVLGSCTSSIACHVISAIVKGQGYSLPGDIQYVDARWEIPVVLPLRDCLTQE 278
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC+LD LK VE+ +GINGL+SSFV +LQ+ENP+RECTIVRTAGKLTPF FN I
Sbjct: 279 LNRLCHLDGLKTVELHKVPYSGINGLVSSFVTLLQKENPSRECTIVRTAGKLTPFHFNSI 338
Query: 1084 PEANVDDXXXXXXISSPRGILSYLQQPNELL 1176
PE + + +++ R + Y +PNE L
Sbjct: 339 PEKSDSN----TTLATQRRLKKYNLKPNESL 365
Score = 108 bits (271), Expect = 1e-21
Identities = 52/98 (53%), Positives = 71/98 (72%), Gaps = 11/98 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPM--------SCYKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQF 1276
ESL E+FCP+CNSP+ S +N Q +IFGA CCSSC++QILP++PSSM+ F
Sbjct: 363 ESLSSESFCPVCNSPLNKSDLLRQSNLQNHQTSSDIFGAACCSSCRFQILPKDPSSMEHF 422
Query: 1277 YSFLPESIISRAKN---GNEKWIREKIQDCLLSDGEDE 1381
Y+ LP+ +++RAK+ GN +RE+IQDCL+SD EDE
Sbjct: 423 YTHLPQPLVARAKHSGYGNLSLLREQIQDCLISDSEDE 460
>ref|XP_021282370.1| cytoplasmic tRNA 2-thiolation protein 2 [Herrania umbratica]
Length = 462
Score = 430 bits (1105), Expect = e-143
Identities = 214/302 (70%), Positives = 251/302 (83%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCKS+E G RFC DCF++NLYGKFK AV+ NAMI+P DKVLVAFSGG SS
Sbjct: 43 CVKCKSNEFVCCGIGGDDARFCKDCFKTNLYGKFKQAVSYNAMITPSDKVLVAFSGGPSS 102
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ +AQKNFDA+ +DRSLPVFGVGVAFIDES +H AS +KAIEDI+
Sbjct: 103 RVVLQFVHEMQCRAQKNFDAS--KDRSLPVFGVGVAFIDESSIHSFASQGAEKAIEDIRS 160
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV LAPP KEL+VVPI +I+S+ DGKE L+ L++ V+D TGKEDLL HLRM+SLQK+
Sbjct: 161 IVSKLAPPEKELYVVPIENIFSSDNIDGKERLKKLLDAVSDVTGKEDLLIHLRMLSLQKV 220
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+EN YT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RWE+PVVLPLRDC QE
Sbjct: 221 ASENRYTRVVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCPAQE 280
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC+LD LKIVE+ N + INGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 281 LNTLCSLDGLKIVELLNGPWSSINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRI 340
Query: 1084 PE 1089
P+
Sbjct: 341 PD 342
Score = 90.9 bits (224), Expect = 1e-15
Identities = 47/97 (48%), Positives = 66/97 (68%), Gaps = 10/97 (10%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPMSCYKNPQPL--------DNIFGATCCSSCQYQILPEEPSSMDQFY 1279
SL E+FCPICNSP++ L +IF A CSSC++QI+P+EPS +QF
Sbjct: 366 SLSSESFCPICNSPLNRSNFSSSLRSLESNKNSDIFAAAACSSCRFQIIPKEPSLTEQFV 425
Query: 1280 SFLPESIISRAKNGN--EKWIREKIQDCLLSDGEDEV 1384
S LP+ +I+RAK+G+ + +RE+IQ+ LLSDGEDE+
Sbjct: 426 SLLPQPLITRAKHGDRGDFSLREQIQEFLLSDGEDEI 462
>ref|XP_012491365.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
[Gossypium raimondii]
ref|XP_012491367.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
[Gossypium raimondii]
gb|KJB43135.1| hypothetical protein B456_007G186200 [Gossypium raimondii]
gb|KJB43136.1| hypothetical protein B456_007G186200 [Gossypium raimondii]
Length = 461
Score = 429 bits (1104), Expect = e-143
Identities = 217/303 (71%), Positives = 253/303 (83%), Gaps = 1/303 (0%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCK +E G RFC DCF++NLYGKFK AVT NAMI+P DKVLVAFSGG SS
Sbjct: 41 CVKCKVNEPVCCGIGGENSRFCKDCFKNNLYGKFKQAVTYNAMITPPDKVLVAFSGGPSS 100
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ KAQKN+DA N+D+SLPVFGVGVAFIDES H S ++KAIEDI+
Sbjct: 101 RVVLQFVHEMQYKAQKNYDA--NKDKSLPVFGVGVAFIDESSTHSFTSQHIEKAIEDIRL 158
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NLAPP+KEL VVPI SI+S+ DGKE L+ L++ V+D TGKEDLL+HLR++ LQKI
Sbjct: 159 IVSNLAPPSKELFVVPIESIFSSDGIDGKERLKELLDAVSDVTGKEDLLSHLRILLLQKI 218
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGYT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RWE+PVVLPLRDC QE
Sbjct: 219 ASENGYTRIVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCPAQE 278
Query: 904 INILCNLD-SLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNR 1080
+N LC+LD SLKIVE+ N +GINGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNR
Sbjct: 279 LNTLCSLDGSLKIVELLNGPCSGINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNR 338
Query: 1081 IPE 1089
+PE
Sbjct: 339 VPE 341
Score = 85.9 bits (211), Expect = 4e-14
Identities = 45/98 (45%), Positives = 66/98 (67%), Gaps = 11/98 (11%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPMSCYKNPQPL--------DNIFGATCCSSCQYQILPEEPSSMDQFY 1279
SL ++FCPICNSP+ P L ++F A+ CSSCQ+QILP++PS M++F
Sbjct: 365 SLSSDSFCPICNSPLKKSNFPSSLRSHGSQQNPDLF-ASACSSCQFQILPKDPSLMEEFL 423
Query: 1280 SFLPESIISRAKN---GNEKWIREKIQDCLLSDGEDEV 1384
S LP+ +I++AK+ GN +RE+IQ+ L SDGE+E+
Sbjct: 424 SLLPQQMITKAKHGDQGNFSLLREQIQEFLFSDGENEI 461
>ref|XP_019224820.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Nicotiana
attenuata]
gb|OIT33086.1| cytoplasmic trna 2-thiolation protein 2 [Nicotiana attenuata]
Length = 478
Score = 429 bits (1104), Expect = e-142
Identities = 218/313 (69%), Positives = 253/313 (80%), Gaps = 9/313 (2%)
Frame = +1
Query: 184 CLKCK---------SSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVL 336
C+KCK S+E GE GRFCADCFRSNLYGKF+ AVTSNAMISPLD VL
Sbjct: 51 CVKCKVNKTIAAAASAEDGGDFSGEDSGRFCADCFRSNLYGKFRFAVTSNAMISPLDNVL 110
Query: 337 VAFSGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDL 516
VAFSGG SRVALQF EMQ KAQKN+DA+ DR+LPVFGVGVAFIDE + ++S DL
Sbjct: 111 VAFSGGTCSRVALQFTHEMQSKAQKNYDASP--DRALPVFGVGVAFIDEKAISAVSSDDL 168
Query: 517 DKAIEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTH 696
D+AIE +K I+ +LAPP K+ HVVP +IYS K D K+ L LIN V+D TGKEDLL H
Sbjct: 169 DRAIEKMKLILSDLAPPVKQFHVVPAEAIYSLKAGDAKDRLNELINAVSDVTGKEDLLEH 228
Query: 697 LRMMSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVL 876
LRM+SLQKIA E+GYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVL
Sbjct: 229 LRMLSLQKIAVEHGYTKVLLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVL 288
Query: 877 PLRDCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGK 1056
PLRDCL+ E+N LC+LDSL IVEVFN +Q+GINGL+SSFVK+LQEENP+RE TI+RTAGK
Sbjct: 289 PLRDCLIHELNTLCSLDSLMIVEVFNGTQSGINGLVSSFVKLLQEENPSRESTIMRTAGK 348
Query: 1057 LTPFPFNRIPEAN 1095
LTPF FNR+ E N
Sbjct: 349 LTPFHFNRMSEDN 361
Score = 97.1 bits (240), Expect = 1e-17
Identities = 45/97 (46%), Positives = 68/97 (70%), Gaps = 11/97 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMSC--------YKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQF 1276
++LPPE+FC IC+SP+ + N + N GA CC+SCQ+Q+LPE+PSS++ F
Sbjct: 382 DALPPESFCRICSSPLKISTFVTSISFDNAKTSLNARGAACCASCQFQVLPEDPSSLEHF 441
Query: 1277 YSFLPESIISRAKNG---NEKWIREKIQDCLLSDGED 1378
S LP S++S A++G +++ +RE+I+DCLLSD ED
Sbjct: 442 ISLLPPSMVSHAEDGDHLSQRQLREQIEDCLLSDNED 478
>ref|XP_015089096.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
pennellii]
Length = 478
Score = 429 bits (1103), Expect = e-142
Identities = 215/310 (69%), Positives = 251/310 (80%), Gaps = 6/310 (1%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEG------GGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAF 345
CLKCK + G G GRFCADCFRSNL+GKF+LAVT+NAMISP D VLVAF
Sbjct: 52 CLKCKVNRTIAAADGGGDFGGGDAGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAF 111
Query: 346 SGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKA 525
SGG SRVALQF EMQ KAQKN+DA+ RDR+LPVFGVGVAFIDE + ++S +LD+A
Sbjct: 112 SGGTCSRVALQFTHEMQSKAQKNYDAS--RDRALPVFGVGVAFIDEKAISAVSSDNLDRA 169
Query: 526 IEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRM 705
+E++K IV +LAPP K+ HVVP +IYS D K+ L LINTV D TGKEDLL H RM
Sbjct: 170 VEEMKLIVSDLAPPVKQFHVVPTEAIYSLDAGDSKDRLNELINTVNDVTGKEDLLEHFRM 229
Query: 706 MSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLR 885
+SLQKIA ENGYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVLPLR
Sbjct: 230 LSLQKIAIENGYTKIILGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVLPLR 289
Query: 886 DCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTP 1065
DC + E+N+LC+LDSLK VEVFN ++AGINGLISS VK+LQEENP+RE TI+RTAGKLTP
Sbjct: 290 DCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSLVKLLQEENPSRESTIMRTAGKLTP 349
Query: 1066 FPFNRIPEAN 1095
F FNR+PE N
Sbjct: 350 FHFNRMPEDN 359
Score = 97.4 bits (241), Expect = 9e-18
Identities = 45/97 (46%), Positives = 68/97 (70%), Gaps = 11/97 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMSC--------YKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQF 1276
++LPPE+FCPICNSP+ ++N + N GA CCSSCQ+Q+LP +PSS++ F
Sbjct: 380 DALPPESFCPICNSPLKISSFGTSINFENGKTNPNGIGAACCSSCQFQVLPNDPSSLEHF 439
Query: 1277 YSFLPESIISRAKNG---NEKWIREKIQDCLLSDGED 1378
S LP +++S+A + +++ +RE+I+DCLLSD ED
Sbjct: 440 ISLLPPAMVSQAGDNDRLSQRQLREQIEDCLLSDYED 476
>ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
lycopersicum]
Length = 478
Score = 429 bits (1103), Expect = e-142
Identities = 215/310 (69%), Positives = 252/310 (81%), Gaps = 6/310 (1%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEG------GGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAF 345
CLKCK + G G GRFCADCFRSNL+GKF+LAVT+NAMISP D VLVAF
Sbjct: 52 CLKCKVNRTIAAADGGGDFGGGDAGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAF 111
Query: 346 SGGASSRVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKA 525
SGG SRVALQF EMQ KAQKN+DA+ RDR+LPVFGVGVAFIDE + ++S +LD+A
Sbjct: 112 SGGTCSRVALQFTHEMQSKAQKNYDAS--RDRALPVFGVGVAFIDEKAISAVSSDNLDRA 169
Query: 526 IEDIKGIVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRM 705
+E+++ IV +LAPP K+ HVVP +IYS D K+ L LINTV D TGKEDLL H RM
Sbjct: 170 VEEMELIVSDLAPPVKQFHVVPTEAIYSLDAGDSKDRLNELINTVNDVTGKEDLLEHFRM 229
Query: 706 MSLQKIATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLR 885
+SLQKIA ENGYTK++LG+CTSRIACHVL ATVKG+GYSLAADIQY DARW++PVVLPLR
Sbjct: 230 LSLQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVLPLR 289
Query: 886 DCLMQEINILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTP 1065
DC + E+N+LC+LDSLK VEVFN ++AGINGLISSFVK+LQEENP+RE TI+RTAGKLTP
Sbjct: 290 DCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSFVKLLQEENPSRESTIMRTAGKLTP 349
Query: 1066 FPFNRIPEAN 1095
F FNR+PE N
Sbjct: 350 FHFNRMPEDN 359
Score = 98.6 bits (244), Expect = 4e-18
Identities = 45/97 (46%), Positives = 68/97 (70%), Gaps = 11/97 (11%)
Frame = +2
Query: 1121 ESLPPEAFCPICNSPMSC--------YKNPQPLDNIFGATCCSSCQYQILPEEPSSMDQF 1276
++LPPE+FCPICNSP+ ++N + N GA CC+SCQ+Q+LP +PSS++ F
Sbjct: 380 DALPPESFCPICNSPLKISSFGTSINFENGKTNPNGIGAACCASCQFQVLPNDPSSLEHF 439
Query: 1277 YSFLPESIISRAKNG---NEKWIREKIQDCLLSDGED 1378
S LP S++S+A + +++ +RE+I+DCLLSD ED
Sbjct: 440 ISLLPPSMVSQAGDNDRLSQRQLREQIEDCLLSDNED 476
>ref|XP_022766665.1| cytoplasmic tRNA 2-thiolation protein 2 isoform X1 [Durio zibethinus]
Length = 460
Score = 428 bits (1100), Expect = e-142
Identities = 207/302 (68%), Positives = 255/302 (84%)
Frame = +1
Query: 184 CLKCKSSEATIFTGGEGGGRFCADCFRSNLYGKFKLAVTSNAMISPLDKVLVAFSGGASS 363
C+KCKS+E +FC DCF++N+YGKFK AVT NAMI+P DKVLVAFSGG SS
Sbjct: 40 CVKCKSNEPICCGIDRDDAKFCRDCFKTNIYGKFKQAVTYNAMITPSDKVLVAFSGGPSS 99
Query: 364 RVALQFVSEMQLKAQKNFDATANRDRSLPVFGVGVAFIDESEVHPIASGDLDKAIEDIKG 543
RV LQFV EMQ +AQ+N+DA+ +D+SLPVFGVGVAF+DES +H +S +++KAIEDI+
Sbjct: 100 RVVLQFVHEMQYRAQRNYDAS--KDKSLPVFGVGVAFVDESSIHSFSSQEIEKAIEDIRS 157
Query: 544 IVLNLAPPTKELHVVPIRSIYSTKTNDGKEMLQNLINTVTDATGKEDLLTHLRMMSLQKI 723
IV NLAPP+KEL+VVPI +I+S+ DGKE L+ L++ V+D TGKEDLL HL+++ LQK+
Sbjct: 158 IVSNLAPPSKELYVVPIENIFSSDGFDGKERLKKLLDAVSDDTGKEDLLIHLQLLLLQKV 217
Query: 724 ATENGYTKLVLGSCTSRIACHVLAATVKGQGYSLAADIQYADARWEVPVVLPLRDCLMQE 903
A+ENGYT++VLGSCTSRIACHV++ATVKGQGYSL+ADIQY D+RWE+PVVLPLRDC QE
Sbjct: 218 ASENGYTRVVLGSCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCSAQE 277
Query: 904 INILCNLDSLKIVEVFNDSQAGINGLISSFVKVLQEENPARECTIVRTAGKLTPFPFNRI 1083
+N LC LD LKI+E+ N ++GINGL+SSFVKVLQEENP+RECTIVRTAGKLTPF FNRI
Sbjct: 278 LNTLCGLDGLKILELLNGPRSGINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHFNRI 337
Query: 1084 PE 1089
PE
Sbjct: 338 PE 339
Score = 93.6 bits (231), Expect = 1e-16
Identities = 48/97 (49%), Positives = 64/97 (65%), Gaps = 11/97 (11%)
Frame = +2
Query: 1124 SLPPEAFCPICNSPMSCYKNPQPLDN--------IFGATCCSSCQYQILPEEPSSMDQFY 1279
SL E+FCPICNSP+ L + I A CCSSCQ+QILP+EPS ++QF
Sbjct: 363 SLSSESFCPICNSPLDKSNLSSSLSSLGSHQNSDILAAACCSSCQFQILPKEPSLLEQFV 422
Query: 1280 SFLPESIISRAKNGNE---KWIREKIQDCLLSDGEDE 1381
S LP+ +I++A +G++ +RE+IQD LLSDGEDE
Sbjct: 423 SLLPQPLITKANHGDQGDFSLLREQIQDFLLSDGEDE 459