BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00021779
         (1574 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_022035731.1| E4 SUMO-protein ligase PIAL2-like isoform X4...   279   1e-81
ref|XP_022035729.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   276   2e-80
ref|XP_022035728.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   273   3e-79
ref|XP_023746387.1| E4 SUMO-protein ligase PIAL1-like isoform X3...   255   2e-74
ref|XP_023746385.1| E4 SUMO-protein ligase PIAL1-like isoform X1...   248   8e-72
ref|XP_022035730.1| E4 SUMO-protein ligase PIAL2-like isoform X3...   248   5e-70
ref|XP_023746389.1| E4 SUMO-protein ligase PIAL1-like isoform X5...   239   1e-68
ref|XP_023746386.1| E4 SUMO-protein ligase PIAL1-like isoform X2...   233   7e-66
ref|XP_023746388.1| E4 SUMO-protein ligase PIAL1-like isoform X4...   228   5e-64
emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]   212   4e-56
gb|OVA16945.1| zinc finger protein [Macleaya cordata]                 212   5e-56
ref|XP_019081226.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   208   8e-56
ref|XP_019081225.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   208   2e-55
ref|XP_010661811.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   208   2e-55
emb|CBI40413.3| unnamed protein product, partial [Vitis vinifera]     206   2e-54
emb|CBI34397.3| unnamed protein product, partial [Vitis vinifera]     203   4e-54
emb|CAN73361.1| hypothetical protein VITISV_006165 [Vitis vinifera]   204   1e-53
ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   204   2e-53
ref|XP_010658435.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isof...   203   7e-53
ref|XP_010661825.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   199   1e-51

>ref|XP_022035731.1| E4 SUMO-protein ligase PIAL2-like isoform X4 [Helianthus annuus]
          Length = 759

 Score =  279 bits (713), Expect = 1e-81
 Identities = 172/407 (42%), Positives = 214/407 (52%), Gaps = 53/407 (13%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            Q GWF D DK  LL +A E S G+ + D ++IEP+N H+ +S+I+SRFYP+M VE IL S
Sbjct: 139  QVGWFSDADKHSLLMIAKEVSYGFSSTDKMNIEPSNAHSCVSNIVSRFYPTMKVEHILTS 198

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F V+AG G   ADF+I+    P                                      
Sbjct: 199  FDVEAGYGTCVADFHISVGATPRHCRSVWLLVAQLDNMETAACITSPQNVELRYLFLHSF 258

Query: 1252 -------------NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSD 1112
                         N+GKGPQLPS+VTKM+K GVN LQVFG+FDG Y+IALA+MN +SSSD
Sbjct: 259  LMNGYEVPNRTKSNMGKGPQLPSDVTKMIKYGVNLLQVFGDFDGRYIIALAFMNSVSSSD 318

Query: 1111 SPQLQDYVYPRSSDVSSDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFM 941
            S  LQDY+ P S  V SDC I   +  ISL CP S  RI  PVKGHLCKH QCFDY  F+
Sbjct: 319  SSLLQDYLLPMS--VDSDCAIIDMSSRISLYCPISKQRIKTPVKGHLCKHPQCFDYNHFV 376

Query: 940  KANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAI 761
            + NS  PTWKCPL  C  P+ N D+RIDQ FLKILNEVAE+V  V IS++GSW V  +  
Sbjct: 377  EVNSAMPTWKCPL--CNNPVCNQDLRIDQDFLKILNEVAEDVHDVVISEDGSWMVAETQS 434

Query: 760  DQPTMTTPAVSPHIVKDTSLVSQGSSDKVNTPSQIQETRLDPVNSTPTQLSAEQPQNSQA 581
            D         S H      +VS G S +VN  +  Q         T   L   Q   S  
Sbjct: 435  D--------TSSHATFTAPVVSLGQS-QVNACANKQAQSTTVFTPTAGSLGQTQVNTSAI 485

Query: 580  DQASEPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPPVVMQLR 440
            +Q S  +   ++     ST N+ +  T      +  P   P   Q+R
Sbjct: 486  NQGSSDQIRVNV-----STLNQGQSTTFTPAATVIKPEVSPGETQVR 527


>ref|XP_022035729.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Helianthus annuus]
 gb|OTG29309.1| putative zinc finger, MIZ-type, Zinc finger, RING/FYVE/PHD-type, E3
            SUMO protein ligase [Helianthus annuus]
          Length = 788

 Score =  276 bits (707), Expect = 2e-80
 Identities = 176/422 (41%), Positives = 224/422 (53%), Gaps = 68/422 (16%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            Q GWF D DK  LL +A E S G+ + D ++IEP+N H+ +S+I+SRFYP+M VE IL S
Sbjct: 139  QVGWFSDADKHSLLMIAKEVSYGFSSTDKMNIEPSNAHSCVSNIVSRFYPTMKVEHILTS 198

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F V+AG G   ADF+I+    P                                      
Sbjct: 199  FDVEAGYGTCVADFHISVGATPRHCRSVWLLVAQLDNMETAACITSPQNVDFLMNGYEVP 258

Query: 1252 -----NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
                 N+GKGPQLPS+VTKM+K GVN LQVFG+FDG Y+IALA+MN +SSSDS  LQDY+
Sbjct: 259  NRTKSNMGKGPQLPSDVTKMIKYGVNLLQVFGDFDGRYIIALAFMNSVSSSDSSLLQDYL 318

Query: 1087 YPRSSDVSSDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPT 917
             P S  V SDC I   +  ISL CP S  RI  PVKGHLCKH QCFDY  F++ NS  PT
Sbjct: 319  LPMS--VDSDCAIIDMSSRISLYCPISKQRIKTPVKGHLCKHPQCFDYNHFVEVNSAMPT 376

Query: 916  WKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAID---QPTM 746
            WKCPL  C  P+ N D+RIDQ FLKILNEVAE+V  V IS++GSW V  +  D     T 
Sbjct: 377  WKCPL--CNNPVCNQDLRIDQDFLKILNEVAEDVHDVVISEDGSWMVAETQSDTSSHATF 434

Query: 745  TTPAVSPHIVKDTSLVS-QGSSDKVNTP-------SQIQETRLDPVNS----TPTQLSAE 602
            T P VS    +  +  + Q  S  V TP       +Q+  + ++   S    TP  +S  
Sbjct: 435  TAPVVSLGQSQVNACANKQAQSTTVFTPTAGSLGQTQVNTSAINQGQSISMFTPPAVSLG 494

Query: 601  QPQNS--------QADQASEPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPPVVMQ 446
            Q Q +             + P  S+   +   ST N+ +  T      +  P   P   Q
Sbjct: 495  QTQVNISTTTHVKSMTMLTSPAGSSDQIRVNVSTLNQGQSTTFTPAATVIKPEVSPGETQ 554

Query: 445  LR 440
            +R
Sbjct: 555  VR 556


>ref|XP_022035728.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Helianthus annuus]
          Length = 796

 Score =  273 bits (699), Expect = 3e-79
 Identities = 176/430 (40%), Positives = 224/430 (52%), Gaps = 76/430 (17%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            Q GWF D DK  LL +A E S G+ + D ++IEP+N H+ +S+I+SRFYP+M VE IL S
Sbjct: 139  QVGWFSDADKHSLLMIAKEVSYGFSSTDKMNIEPSNAHSCVSNIVSRFYPTMKVEHILTS 198

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F V+AG G   ADF+I+    P                                      
Sbjct: 199  FDVEAGYGTCVADFHISVGATPRHCRSVWLLVAQLDNMETAACITSPQNVELRYLFLHSF 258

Query: 1252 -------------NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSD 1112
                         N+GKGPQLPS+VTKM+K GVN LQVFG+FDG Y+IALA+MN +SSSD
Sbjct: 259  LMNGYEVPNRTKSNMGKGPQLPSDVTKMIKYGVNLLQVFGDFDGRYIIALAFMNSVSSSD 318

Query: 1111 SPQLQDYVYPRSSDVSSDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFM 941
            S  LQDY+ P S  V SDC I   +  ISL CP S  RI  PVKGHLCKH QCFDY  F+
Sbjct: 319  SSLLQDYLLPMS--VDSDCAIIDMSSRISLYCPISKQRIKTPVKGHLCKHPQCFDYNHFV 376

Query: 940  KANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAI 761
            + NS  PTWKCPL  C  P+ N D+RIDQ FLKILNEVAE+V  V IS++GSW V  +  
Sbjct: 377  EVNSAMPTWKCPL--CNNPVCNQDLRIDQDFLKILNEVAEDVHDVVISEDGSWMVAETQS 434

Query: 760  D---QPTMTTPAVSPHIVKDTSLVS-QGSSDKVNTP-------SQIQETRLDPVNS---- 626
            D     T T P VS    +  +  + Q  S  V TP       +Q+  + ++   S    
Sbjct: 435  DTSSHATFTAPVVSLGQSQVNACANKQAQSTTVFTPTAGSLGQTQVNTSAINQGQSISMF 494

Query: 625  TPTQLSAEQPQNS--------QADQASEPESSTSITKRARSTFNRARRATIVIPVQLTPP 470
            TP  +S  Q Q +             + P  S+   +   ST N+ +  T      +  P
Sbjct: 495  TPPAVSLGQTQVNISTTTHVKSMTMLTSPAGSSDQIRVNVSTLNQGQSTTFTPAATVIKP 554

Query: 469  SAPPVVMQLR 440
               P   Q+R
Sbjct: 555  EVSPGETQVR 564


>ref|XP_023746387.1| E4 SUMO-protein ligase PIAL1-like isoform X3 [Lactuca sativa]
 gb|PLY64200.1| hypothetical protein LSAT_7X2680 [Lactuca sativa]
          Length = 563

 Score =  255 bits (652), Expect = 2e-74
 Identities = 163/397 (41%), Positives = 216/397 (54%), Gaps = 49/397 (12%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            + GWF D DK  LL + +E   G+ + + + IEP   H+ +S+II RFYP+M VE IL S
Sbjct: 111  KVGWFSDGDKADLLMIVEEVCKGFSDTEKMSIEPNYAHSCVSNIILRFYPTMKVENILTS 170

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F VKAG G    DF+I+  T+P                                      
Sbjct: 171  FDVKAGYGTFVVDFHISQGTLPRNCRNLWLLVARMDNMDTVACIANPSNVDFLINGKGVP 230

Query: 1252 -----NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
                  +GKGPQLPSNVTKM+K GVN LQV G+FDG Y+IA+A+MNL  S D P L+DYV
Sbjct: 231  KRTKSTMGKGPQLPSNVTKMIKYGVNLLQVLGDFDGQYIIAVAFMNLNPSPDLPPLKDYV 290

Query: 1087 YPRSSDVSSDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPT 917
            +  S+ V SDC +   +  ISL CP S  RI  PVKGHLCKH QCFDY++F++ NS  PT
Sbjct: 291  HTTSTVVDSDCNVIGTSSLISLDCPISKLRIRTPVKGHLCKHPQCFDYKYFLEVNSRWPT 350

Query: 916  WKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMTTP 737
            W CP+  C  P+  LD+RIDQ F+K+LNEVAE+VS V +S  GSWTV      Q   +  
Sbjct: 351  WNCPI--CNIPLCYLDVRIDQTFVKMLNEVAEDVSNVMVSANGSWTVATKTKRQTIESIT 408

Query: 736  AVSPHIVKDTSLVSQG--SSDKVNT-PSQIQETRLDPVNSTPTQLSAEQPQNSQAD-QAS 569
              + H V ++SL SQ   ++D+ +  PS  ++ +   V      L     Q    D   S
Sbjct: 409  RNNNH-VPNSSLPSQSLHTTDQADAGPSTTEQAQSTTVALAFEHLRGGGEQQHYTDLDPS 467

Query: 568  EPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPP 458
            +P+  T  T++A+ST       +   PV   P  APP
Sbjct: 468  QPDVGT--TEQAQST-----TISTPPPVGSQPQQAPP 497


>ref|XP_023746385.1| E4 SUMO-protein ligase PIAL1-like isoform X1 [Lactuca sativa]
          Length = 570

 Score =  248 bits (634), Expect = 8e-72
 Identities = 163/404 (40%), Positives = 216/404 (53%), Gaps = 56/404 (13%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            + GWF D DK  LL + +E   G+ + + + IEP   H+ +S+II RFYP+M VE IL S
Sbjct: 111  KVGWFSDGDKADLLMIVEEVCKGFSDTEKMSIEPNYAHSCVSNIILRFYPTMKVENILTS 170

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F VKAG G    DF+I+  T+P                                      
Sbjct: 171  FDVKAGYGTFVVDFHISQGTLPRNCRNLWLLVARMDNMDTVACIANPSNVDFLINGKGVP 230

Query: 1252 -----NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
                  +GKGPQLPSNVTKM+K GVN LQV G+FDG Y+IA+A+MNL  S D P L+DYV
Sbjct: 231  KRTKSTMGKGPQLPSNVTKMIKYGVNLLQVLGDFDGQYIIAVAFMNLNPSPDLPPLKDYV 290

Query: 1087 YPRSSDVS-------SDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMK 938
            +  S+ V        SDC +   +  ISL CP S  RI  PVKGHLCKH QCFDY++F++
Sbjct: 291  HTTSTVVDSVLVFPVSDCNVIGTSSLISLDCPISKLRIRTPVKGHLCKHPQCFDYKYFLE 350

Query: 937  ANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAID 758
             NS  PTW CP+  C  P+  LD+RIDQ F+K+LNEVAE+VS V +S  GSWTV      
Sbjct: 351  VNSRWPTWNCPI--CNIPLCYLDVRIDQTFVKMLNEVAEDVSNVMVSANGSWTVATKTKR 408

Query: 757  QPTMTTPAVSPHIVKDTSLVSQG--SSDKVNT-PSQIQETRLDPVNSTPTQLSAEQPQNS 587
            Q   +    + H V ++SL SQ   ++D+ +  PS  ++ +   V      L     Q  
Sbjct: 409  QTIESITRNNNH-VPNSSLPSQSLHTTDQADAGPSTTEQAQSTTVALAFEHLRGGGEQQH 467

Query: 586  QAD-QASEPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPP 458
              D   S+P+  T  T++A+ST       +   PV   P  APP
Sbjct: 468  YTDLDPSQPDVGT--TEQAQST-----TISTPPPVGSQPQQAPP 504


>ref|XP_022035730.1| E4 SUMO-protein ligase PIAL2-like isoform X3 [Helianthus annuus]
          Length = 784

 Score =  248 bits (634), Expect = 5e-70
 Identities = 168/430 (39%), Positives = 214/430 (49%), Gaps = 76/430 (17%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            Q GWF D DK  LL +A E S G+ + D ++IEP+N H+ +S+I+SRFYP+M VE IL S
Sbjct: 139  QVGWFSDADKHSLLMIAKEVSYGFSSTDKMNIEPSNAHSCVSNIVSRFYPTMKVEHILTS 198

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F V+AG G   ADF+I+    P                                      
Sbjct: 199  FDVEAGYGTCVADFHISVGATPRHCRSVWLLVAQLDNMETAACITSPQNVELRYLFLHSF 258

Query: 1252 -------------NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSD 1112
                         N+GKGPQLPS+VTKM+K GVN LQVFG+FD            +SSSD
Sbjct: 259  LMNGYEVPNRTKSNMGKGPQLPSDVTKMIKYGVNLLQVFGDFD------------VSSSD 306

Query: 1111 SPQLQDYVYPRSSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFM 941
            S  LQDY+ P S D  SDC I +    ISL CP S  RI  PVKGHLCKH QCFDY  F+
Sbjct: 307  SSLLQDYLLPMSVD--SDCAIIDMSSRISLYCPISKQRIKTPVKGHLCKHPQCFDYNHFV 364

Query: 940  KANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAI 761
            + NS  PTWKCPL  C  P+ N D+RIDQ FLKILNEVAE+V  V IS++GSW V  +  
Sbjct: 365  EVNSAMPTWKCPL--CNNPVCNQDLRIDQDFLKILNEVAEDVHDVVISEDGSWMVAETQS 422

Query: 760  D---QPTMTTPAVSPHIVKDTSLVS-QGSSDKVNTP-------SQIQETRLDPVNS---- 626
            D     T T P VS    +  +  + Q  S  V TP       +Q+  + ++   S    
Sbjct: 423  DTSSHATFTAPVVSLGQSQVNACANKQAQSTTVFTPTAGSLGQTQVNTSAINQGQSISMF 482

Query: 625  TPTQLSAEQPQNS--------QADQASEPESSTSITKRARSTFNRARRATIVIPVQLTPP 470
            TP  +S  Q Q +             + P  S+   +   ST N+ +  T      +  P
Sbjct: 483  TPPAVSLGQTQVNISTTTHVKSMTMLTSPAGSSDQIRVNVSTLNQGQSTTFTPAATVIKP 542

Query: 469  SAPPVVMQLR 440
               P   Q+R
Sbjct: 543  EVSPGETQVR 552


>ref|XP_023746389.1| E4 SUMO-protein ligase PIAL1-like isoform X5 [Lactuca sativa]
          Length = 557

 Score =  239 bits (611), Expect = 1e-68
 Identities = 159/397 (40%), Positives = 211/397 (53%), Gaps = 49/397 (12%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            + GWF D DK  LL + +E   G+ + + + IEP   H+ +S+II RFYP+M VE IL S
Sbjct: 111  KVGWFSDGDKADLLMIVEEVCKGFSDTEKMSIEPNYAHSCVSNIILRFYPTMKVENILTS 170

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F VKAG G    DF+I+  T+P                                      
Sbjct: 171  FDVKAGYGTFVVDFHISQGTLPRNCRNLWLLVARMDNMDTVACIANPSNVDFLINGKGVP 230

Query: 1252 -----NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
                  +GKGPQLPSNVTKM+K GVN LQV G+FD      +A+MNL  S D P L+DYV
Sbjct: 231  KRTKSTMGKGPQLPSNVTKMIKYGVNLLQVLGDFD------VAFMNLNPSPDLPPLKDYV 284

Query: 1087 YPRSSDVSSDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPT 917
            +  S+ V SDC +   +  ISL CP S  RI  PVKGHLCKH QCFDY++F++ NS  PT
Sbjct: 285  HTTSTVVDSDCNVIGTSSLISLDCPISKLRIRTPVKGHLCKHPQCFDYKYFLEVNSRWPT 344

Query: 916  WKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMTTP 737
            W CP+  C  P+  LD+RIDQ F+K+LNEVAE+VS V +S  GSWTV      Q   +  
Sbjct: 345  WNCPI--CNIPLCYLDVRIDQTFVKMLNEVAEDVSNVMVSANGSWTVATKTKRQTIESIT 402

Query: 736  AVSPHIVKDTSLVSQG--SSDKVNT-PSQIQETRLDPVNSTPTQLSAEQPQNSQAD-QAS 569
              + H V ++SL SQ   ++D+ +  PS  ++ +   V      L     Q    D   S
Sbjct: 403  RNNNH-VPNSSLPSQSLHTTDQADAGPSTTEQAQSTTVALAFEHLRGGGEQQHYTDLDPS 461

Query: 568  EPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPP 458
            +P+  T  T++A+ST       +   PV   P  APP
Sbjct: 462  QPDVGT--TEQAQST-----TISTPPPVGSQPQQAPP 491


>ref|XP_023746386.1| E4 SUMO-protein ligase PIAL1-like isoform X2 [Lactuca sativa]
          Length = 564

 Score =  233 bits (593), Expect = 7e-66
 Identities = 159/404 (39%), Positives = 211/404 (52%), Gaps = 56/404 (13%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            + GWF D DK  LL + +E   G+ + + + IEP   H+ +S+II RFYP+M VE IL S
Sbjct: 111  KVGWFSDGDKADLLMIVEEVCKGFSDTEKMSIEPNYAHSCVSNIILRFYPTMKVENILTS 170

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F VKAG G    DF+I+  T+P                                      
Sbjct: 171  FDVKAGYGTFVVDFHISQGTLPRNCRNLWLLVARMDNMDTVACIANPSNVDFLINGKGVP 230

Query: 1252 -----NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
                  +GKGPQLPSNVTKM+K GVN LQV G+FD      +A+MNL  S D P L+DYV
Sbjct: 231  KRTKSTMGKGPQLPSNVTKMIKYGVNLLQVLGDFD------VAFMNLNPSPDLPPLKDYV 284

Query: 1087 YPRSSDVSS-------DCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMK 938
            +  S+ V S       DC +   +  ISL CP S  RI  PVKGHLCKH QCFDY++F++
Sbjct: 285  HTTSTVVDSVLVFPVSDCNVIGTSSLISLDCPISKLRIRTPVKGHLCKHPQCFDYKYFLE 344

Query: 937  ANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAID 758
             NS  PTW CP+  C  P+  LD+RIDQ F+K+LNEVAE+VS V +S  GSWTV      
Sbjct: 345  VNSRWPTWNCPI--CNIPLCYLDVRIDQTFVKMLNEVAEDVSNVMVSANGSWTVATKTKR 402

Query: 757  QPTMTTPAVSPHIVKDTSLVSQG--SSDKVNT-PSQIQETRLDPVNSTPTQLSAEQPQNS 587
            Q   +    + H V ++SL SQ   ++D+ +  PS  ++ +   V      L     Q  
Sbjct: 403  QTIESITRNNNH-VPNSSLPSQSLHTTDQADAGPSTTEQAQSTTVALAFEHLRGGGEQQH 461

Query: 586  QAD-QASEPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPP 458
              D   S+P+  T  T++A+ST       +   PV   P  APP
Sbjct: 462  YTDLDPSQPDVGT--TEQAQST-----TISTPPPVGSQPQQAPP 498


>ref|XP_023746388.1| E4 SUMO-protein ligase PIAL1-like isoform X4 [Lactuca sativa]
          Length = 561

 Score =  228 bits (580), Expect = 5e-64
 Identities = 157/404 (38%), Positives = 208/404 (51%), Gaps = 56/404 (13%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHT-ISDIISRFYPSMTVEQILIS 1325
            + GWF D DK  LL + +E   G+ + + + IEP   H+ +S+II RFYP+M VE IL S
Sbjct: 111  KVGWFSDGDKADLLMIVEEVCKGFSDTEKMSIEPNYAHSCVSNIILRFYPTMKVENILTS 170

Query: 1324 FAVKAGEGPPEADFYIAPDTVPNQ------------------------------------ 1253
            F VKAG G    DF+I+  T+P                                      
Sbjct: 171  FDVKAGYGTFVVDFHISQGTLPRNCRNLWLLVARMDNMDTVACIANPSNVDFLINGKGVP 230

Query: 1252 -----NVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
                  +GKGPQLPSNVTKM+K GVN LQV G+FDG Y+IA+A+MNL  S D P L+DYV
Sbjct: 231  KRTKSTMGKGPQLPSNVTKMIKYGVNLLQVLGDFDGQYIIAVAFMNLNPSPDLPPLKDYV 290

Query: 1087 YPRSSDVS-------SDCVI---TEEISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMK 938
            +  S+ V        SDC +   +  ISL CP S  RI  PVKGHLCKH QCFDY++F++
Sbjct: 291  HTTSTVVDSVLVFPVSDCNVIGTSSLISLDCPISKLRIRTPVKGHLCKHPQCFDYKYFLE 350

Query: 937  ANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAID 758
             NS  PTW CP+  C  P+  LD         +LNEVAE+VS V +S  GSWTV      
Sbjct: 351  VNSRWPTWNCPI--CNIPLCYLD---------MLNEVAEDVSNVMVSANGSWTVATKTKR 399

Query: 757  QPTMTTPAVSPHIVKDTSLVSQG--SSDKVNT-PSQIQETRLDPVNSTPTQLSAEQPQNS 587
            Q   +    + H V ++SL SQ   ++D+ +  PS  ++ +   V      L     Q  
Sbjct: 400  QTIESITRNNNH-VPNSSLPSQSLHTTDQADAGPSTTEQAQSTTVALAFEHLRGGGEQQH 458

Query: 586  QAD-QASEPESSTSITKRARSTFNRARRATIVIPVQLTPPSAPP 458
              D   S+P+  T  T++A+ST       +   PV   P  APP
Sbjct: 459  YTDLDPSQPDVGT--TEQAQST-----TISTPPPVGSQPQQAPP 495


>emb|CAN82727.1| hypothetical protein VITISV_040324 [Vitis vinifera]
          Length = 901

 Score =  212 bits (539), Expect = 4e-56
 Identities = 120/263 (45%), Positives = 162/263 (61%), Gaps = 9/263 (3%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGH-TISDIISRFYPSMTVEQILIS 1325
            + GWF ++D + LL + +E    + NL + + EP + H TIS I++RFYP M + QIL S
Sbjct: 141  KVGWFTEKDTEELLTLVNEIGSNFCNLGDNNTEPISFHPTISKIMTRFYPRMEMGQILAS 200

Query: 1324 FAVKAGEGPPEADFYIAPDTV--PNQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYV 1151
              VK G G    DF+I+  T     + +  GPQ+P+NVT M+K G N LQ  G+F+G Y+
Sbjct: 201  REVKPGYGTFLVDFHISKSTKFSSQEKIDSGPQIPTNVTPMLKYGTNLLQAVGQFNGHYI 260

Query: 1150 IALAYMNLISSSDSPQLQDYVYPRSSDVSSDCVITE---EISLICPSSGCRISMPVKGHL 980
            +A+A+M +ISS D+P LQDYV P  S + SD  I E    ISL CP S  RI +PVKGH 
Sbjct: 261  LAIAFMAVISSPDNPVLQDYVQPAVSMLHSDNEIVEGPSRISLNCPISRTRIKVPVKGHS 320

Query: 979  CKHSQCFDYEFFMKANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYI 800
            CKH QCFD+  F++ NS RP+W+CP   C + +   DIRIDQ    +L EV ENV+ V I
Sbjct: 321  CKHLQCFDFGNFVEINSRRPSWRCP--HCNQYVCYTDIRIDQNM--VLKEVGENVADVII 376

Query: 799  SDEGSWTV---DPSAIDQPTMTT 740
            S +GSW         +DQP + T
Sbjct: 377  SADGSWKAILESNDHVDQPRVGT 399


>gb|OVA16945.1| zinc finger protein [Macleaya cordata]
          Length = 928

 Score =  212 bits (539), Expect = 5e-56
 Identities = 137/373 (36%), Positives = 182/373 (48%), Gaps = 47/373 (12%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNG-HTISDIISRFYPSMTVEQILIS 1325
            ++GWFL +D D LL   +E  +G+ N  ++  EP+N    IS I+SRFYP M +  I  S
Sbjct: 120  KSGWFLVKDADELLNRTNELGMGFCNPGDIITEPSNPPQIISKILSRFYPWMKMSHIFAS 179

Query: 1324 FAVKAGEGPPEADFYIAPDTVP-------------------------------------- 1259
              VK G G    DF+I+ +                                         
Sbjct: 180  LEVKPGFGAYLIDFHISKNKASSAREKIRLFAAQTDNIETSSCIISPPRVNFLLNGKGIE 239

Query: 1258 ---NQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
               N  +  GPQ P+NVT MVK G N LQ  G F+G+Y+IA+A+ + +SS D P+LQDYV
Sbjct: 240  RRNNVTMDNGPQFPTNVTPMVKYGTNLLQAVGHFNGNYIIAIAFTSEVSSVDIPELQDYV 299

Query: 1087 YPRSSDVSSDCVITEE---ISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPT 917
             P S+ + SD  I EE   ISL CP S  RI  PVKGHLCKH QCFDYE F+K NS RP+
Sbjct: 300  RPLSTALDSDSEIIEEASRISLNCPISFRRIKTPVKGHLCKHHQCFDYENFVKINSRRPS 359

Query: 916  WKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMTTP 737
            W+CP   C + +   D+RIDQ  +++L EV ENV+ V IS +GSW               
Sbjct: 360  WRCP--QCNQSVCYTDLRIDQCMVEVLKEVGENVADVTISADGSW--------------- 402

Query: 736  AVSPHIVKDTSLVSQGSS--DKVNTPSQIQETRLDPVNSTPTQLSAEQPQNSQADQASEP 563
                HI  D S   Q  +  +K +   Q + TR     +    L+ E    + A   SEP
Sbjct: 403  --KAHIETDDSTDQQDDTTRNKQDESDQCEPTRFSTSIANVVDLTVEGVDENNAMDISEP 460

Query: 562  ESSTSITKRARST 524
            E    +      T
Sbjct: 461  EDRKPLKDTVHGT 473


>ref|XP_019081226.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X4 [Vitis vinifera]
          Length = 694

 Score =  208 bits (530), Expect = 8e-56
 Identities = 118/295 (40%), Positives = 166/295 (56%), Gaps = 44/295 (14%)
 Frame = -2

Query: 1495 GWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHTISDIISRFYPSMTVEQILISFAV 1316
            GWF D D + LL +A E    +  +++++++P+    +  I+ R+YP   +  +L S  V
Sbjct: 24   GWFPDHDANDLLALAKEIGKNFSTMEDINVQPSYLLNVWKIMLRYYPRFRMGHMLASLDV 83

Query: 1315 KAGEG--------------------------------------PPEADFYIAPDTVP--- 1259
            K G G                                      PP+A+F +    V    
Sbjct: 84   KPGYGAFVVDFHITKSMLSPAQKRICLFVAQTDNMDTSSCIVLPPQANFMLNGQGVRGRI 143

Query: 1258 NQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYVYPR 1079
            N ++  GPQLP+NVT M+K G N LQV G+F+G+YVI +A+M++IS+S++P+LQDY+ P 
Sbjct: 144  NGSMDNGPQLPTNVTAMLKYGKNLLQVVGQFNGNYVIVIAFMSMISTSNTPELQDYIQPV 203

Query: 1078 SSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPTWKC 908
            +    SD  + E    ISL CP S  RI +PVKGHLCKH QCFDY  FM+ NS RP+W+C
Sbjct: 204  AVTPDSDLEVIEGQARISLNCPISFKRIKIPVKGHLCKHHQCFDYGNFMEINSRRPSWRC 263

Query: 907  PLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMT 743
            P   C +P+ N DIRIDQK +K+L EV ENV  V IS +GSW     +ID  T +
Sbjct: 264  P--HCNQPVCNPDIRIDQKMVKVLKEVEENVVDVIISPDGSWKPVVESIDHETQS 316


>ref|XP_019081225.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X3 [Vitis vinifera]
          Length = 756

 Score =  208 bits (530), Expect = 2e-55
 Identities = 118/295 (40%), Positives = 166/295 (56%), Gaps = 44/295 (14%)
 Frame = -2

Query: 1495 GWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHTISDIISRFYPSMTVEQILISFAV 1316
            GWF D D + LL +A E    +  +++++++P+    +  I+ R+YP   +  +L S  V
Sbjct: 86   GWFPDHDANDLLALAKEIGKNFSTMEDINVQPSYLLNVWKIMLRYYPRFRMGHMLASLDV 145

Query: 1315 KAGEG--------------------------------------PPEADFYIAPDTVP--- 1259
            K G G                                      PP+A+F +    V    
Sbjct: 146  KPGYGAFVVDFHITKSMLSPAQKRICLFVAQTDNMDTSSCIVLPPQANFMLNGQGVRGRI 205

Query: 1258 NQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYVYPR 1079
            N ++  GPQLP+NVT M+K G N LQV G+F+G+YVI +A+M++IS+S++P+LQDY+ P 
Sbjct: 206  NGSMDNGPQLPTNVTAMLKYGKNLLQVVGQFNGNYVIVIAFMSMISTSNTPELQDYIQPV 265

Query: 1078 SSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPTWKC 908
            +    SD  + E    ISL CP S  RI +PVKGHLCKH QCFDY  FM+ NS RP+W+C
Sbjct: 266  AVTPDSDLEVIEGQARISLNCPISFKRIKIPVKGHLCKHHQCFDYGNFMEINSRRPSWRC 325

Query: 907  PLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMT 743
            P   C +P+ N DIRIDQK +K+L EV ENV  V IS +GSW     +ID  T +
Sbjct: 326  P--HCNQPVCNPDIRIDQKMVKVLKEVEENVVDVIISPDGSWKPVVESIDHETQS 378


>ref|XP_010661811.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_010661814.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_010661817.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_019081218.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_019081219.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_019081220.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_019081221.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_019081222.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
 ref|XP_019081223.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
          Length = 778

 Score =  208 bits (530), Expect = 2e-55
 Identities = 118/295 (40%), Positives = 166/295 (56%), Gaps = 44/295 (14%)
 Frame = -2

Query: 1495 GWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHTISDIISRFYPSMTVEQILISFAV 1316
            GWF D D + LL +A E    +  +++++++P+    +  I+ R+YP   +  +L S  V
Sbjct: 108  GWFPDHDANDLLALAKEIGKNFSTMEDINVQPSYLLNVWKIMLRYYPRFRMGHMLASLDV 167

Query: 1315 KAGEG--------------------------------------PPEADFYIAPDTVP--- 1259
            K G G                                      PP+A+F +    V    
Sbjct: 168  KPGYGAFVVDFHITKSMLSPAQKRICLFVAQTDNMDTSSCIVLPPQANFMLNGQGVRGRI 227

Query: 1258 NQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYVYPR 1079
            N ++  GPQLP+NVT M+K G N LQV G+F+G+YVI +A+M++IS+S++P+LQDY+ P 
Sbjct: 228  NGSMDNGPQLPTNVTAMLKYGKNLLQVVGQFNGNYVIVIAFMSMISTSNTPELQDYIQPV 287

Query: 1078 SSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPTWKC 908
            +    SD  + E    ISL CP S  RI +PVKGHLCKH QCFDY  FM+ NS RP+W+C
Sbjct: 288  AVTPDSDLEVIEGQARISLNCPISFKRIKIPVKGHLCKHHQCFDYGNFMEINSRRPSWRC 347

Query: 907  PLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMT 743
            P   C +P+ N DIRIDQK +K+L EV ENV  V IS +GSW     +ID  T +
Sbjct: 348  P--HCNQPVCNPDIRIDQKMVKVLKEVEENVVDVIISPDGSWKPVVESIDHETQS 400


>emb|CBI40413.3| unnamed protein product, partial [Vitis vinifera]
          Length = 776

 Score =  206 bits (523), Expect = 2e-54
 Identities = 116/293 (39%), Positives = 166/293 (56%), Gaps = 42/293 (14%)
 Frame = -2

Query: 1495 GWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGHTISDIISRFYPSMTVEQILISFAV 1316
            GWF D D + LL +A E    +  +++++++P+    +  I+ R+YP   +  +L S  V
Sbjct: 108  GWFPDHDANDLLALAKEIGKNFSTMEDINVQPSYLLNVWKIMLRYYPRFRMGHMLASLDV 167

Query: 1315 KAGEG--------------------------------------PPEADFYIAPDTVP--- 1259
            K G G                                      PP+A+F +    V    
Sbjct: 168  KPGYGAFVVDFHITKSMLSPAQKRICLFVAQTDNMDTSSCIVLPPQANFMLNGQGVRGRI 227

Query: 1258 NQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYVYPR 1079
            N ++  GPQLP+NVT M+K G N LQV G+F+G+YVI +A+M++IS+S++P+LQDY+ P 
Sbjct: 228  NGSMDNGPQLPTNVTAMLKYGKNLLQVVGQFNGNYVIVIAFMSMISTSNTPELQDYIQPV 287

Query: 1078 SSDVSSDCVITE-EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPTWKCPL 902
            +    S+ +  +  ISL CP S  RI +PVKGHLCKH QCFDY  FM+ NS RP+W+CP 
Sbjct: 288  AVTPDSEVIEGQARISLNCPISFKRIKIPVKGHLCKHHQCFDYGNFMEINSRRPSWRCP- 346

Query: 901  AGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTVDPSAIDQPTMT 743
              C +P+ N DIRIDQK +K+L EV ENV  V IS +GSW     +ID  T +
Sbjct: 347  -HCNQPVCNPDIRIDQKMVKVLKEVEENVVDVIISPDGSWKPVVESIDHETQS 398


>emb|CBI34397.3| unnamed protein product, partial [Vitis vinifera]
          Length = 646

 Score =  203 bits (516), Expect = 4e-54
 Identities = 122/302 (40%), Positives = 164/302 (54%), Gaps = 48/302 (15%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGH-TISDIISRFYPSMTVEQILIS 1325
            + GWF ++D + LL + +E    + NL + + EP + H TIS I++RFYP M + QIL S
Sbjct: 121  KVGWFTEKDTEELLTLVNEIGSNFCNLGDNNTEPISFHPTISKIMTRFYPRMEMGQILAS 180

Query: 1324 FAVKAGEGPPEADFYIAPDTV--------------------------------------- 1262
              VK G G    DF+I+  T                                        
Sbjct: 181  REVKPGYGTFLVDFHISKSTKFSSQEKIRLFVAQTDNIETSSCIITPPQVNFLLNGKGVE 240

Query: 1261 --PNQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
               N  +  GPQ+P+NVT M+K G N LQ  G+F+G Y++A+A+M +ISS D+P LQDYV
Sbjct: 241  RRTNVFMDSGPQIPTNVTPMLKYGTNLLQAVGQFNGHYILAIAFMAVISSPDNPVLQDYV 300

Query: 1087 YPRSSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPT 917
             P  S + SD  I E    ISL CP S  RI +PVKGH CKH QCFD+  F++ NS RP+
Sbjct: 301  QPAVSMLHSDNEIVEGPSRISLNCPISRTRIKVPVKGHSCKHLQCFDFGNFVEINSRRPS 360

Query: 916  WKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTV---DPSAIDQPTM 746
            W+CP   C + +   DIRIDQ  +K+L EV ENV+ V IS +GSW         +DQP +
Sbjct: 361  WRCP--HCNQYVCYTDIRIDQNMVKVLKEVGENVADVIISADGSWKAILESNDHVDQPRV 418

Query: 745  TT 740
             T
Sbjct: 419  GT 420


>emb|CAN73361.1| hypothetical protein VITISV_006165 [Vitis vinifera]
          Length = 845

 Score =  204 bits (520), Expect = 1e-53
 Identities = 114/252 (45%), Positives = 158/252 (62%), Gaps = 6/252 (2%)
 Frame = -2

Query: 1495 GWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNG-HTISDIISRFYPSMTVEQILISFA 1319
            GWFLD D   LL +A E    +  +++++ EP     ++S I+SR+YP + +  +L S  
Sbjct: 105  GWFLDHDATDLLTLAKEIGKIFSTMEDINAEPHYPLPSVSKIMSRYYPRLRMGHVLASLE 164

Query: 1318 VKAGEGPPEADFYIAPDTVP--NQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIA 1145
            VK G G    DF+I    V    +++  GPQLP+NV  M++ G+N LQV G+F+G+YVI 
Sbjct: 165  VKPGYGAFVIDFHITRSMVSPAQKHIDNGPQLPTNVIAMLRYGINLLQVVGQFNGNYVII 224

Query: 1144 LAYMNLISSSDSPQLQDYVYPRSSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCK 974
            +A+M++IS+S  P+LQ+Y+ P +    SD  I E    ISL CP S  RI++PVKGHLCK
Sbjct: 225  IAFMSVISTSGIPELQEYIQPVAVTSDSDLEIIEGQARISLNCPISFRRINIPVKGHLCK 284

Query: 973  HSQCFDYEFFMKANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISD 794
            H QCFDY  F++ NS RP+W+CP   C + + N DIRIDQ    IL EV ENV  V IS 
Sbjct: 285  HHQCFDYGNFIEINSRRPSWRCP--HCNQSVCNPDIRIDQNM--ILREVGENVVDVIISP 340

Query: 793  EGSWTVDPSAID 758
            +GSW     +ID
Sbjct: 341  DGSWKPVVESID 352


>ref|XP_019071879.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X2 [Vitis vinifera]
          Length = 898

 Score =  204 bits (519), Expect = 2e-53
 Identities = 119/278 (42%), Positives = 163/278 (58%), Gaps = 24/278 (8%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGH-TISDIISRFYPSMTVEQILIS 1325
            + GWF ++D + LL + +E    + NL + + EP + H TIS I++RFYP M + QIL S
Sbjct: 121  KVGWFTEKDTEELLTLVNEIGSNFCNLGDNNTEPISFHPTISKIMTRFYPRMEMGQILAS 180

Query: 1324 FAVK--------------AGEGPPEADFYIAPDTVPNQN---VGKGPQLPSNVTKMVKDG 1196
              VK                  PP+ +F +    V  +    +  GPQ+P+NVT M+K G
Sbjct: 181  REVKRLFVAQTDNIETSSCIITPPQVNFLLNGKGVERRTNVFMDSGPQIPTNVTPMLKYG 240

Query: 1195 VNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYVYPRSSDVSSDCVITE---EISLIC 1025
             N LQ  G+F+G Y++A+A+M +ISS D+P LQDYV P  S + SD  I E    ISL C
Sbjct: 241  TNLLQAVGQFNGHYILAIAFMAVISSPDNPVLQDYVQPAVSMLHSDNEIVEGPSRISLNC 300

Query: 1024 PSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPTWKCPLAGCRKPISNLDIRIDQKFL 845
            P S  RI +PVKGH CKH QCFD+  F++ NS RP+W+CP   C + +   DIRIDQ  +
Sbjct: 301  PISRTRIKVPVKGHSCKHLQCFDFGNFVEINSRRPSWRCP--HCNQYVCYTDIRIDQNMV 358

Query: 844  KILNEVAENVSKVYISDEGSWTV---DPSAIDQPTMTT 740
            K+L EV ENV+ V IS +GSW         +DQP + T
Sbjct: 359  KVLKEVGENVADVIISADGSWKAILESNDHVDQPRVGT 396


>ref|XP_010658435.1| PREDICTED: E4 SUMO-protein ligase PIAL1 isoform X1 [Vitis vinifera]
          Length = 922

 Score =  203 bits (516), Expect = 7e-53
 Identities = 122/302 (40%), Positives = 164/302 (54%), Gaps = 48/302 (15%)
 Frame = -2

Query: 1501 QAGWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNGH-TISDIISRFYPSMTVEQILIS 1325
            + GWF ++D + LL + +E    + NL + + EP + H TIS I++RFYP M + QIL S
Sbjct: 121  KVGWFTEKDTEELLTLVNEIGSNFCNLGDNNTEPISFHPTISKIMTRFYPRMEMGQILAS 180

Query: 1324 FAVKAGEGPPEADFYIAPDTV--------------------------------------- 1262
              VK G G    DF+I+  T                                        
Sbjct: 181  REVKPGYGTFLVDFHISKSTKFSSQEKIRLFVAQTDNIETSSCIITPPQVNFLLNGKGVE 240

Query: 1261 --PNQNVGKGPQLPSNVTKMVKDGVNNLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYV 1088
               N  +  GPQ+P+NVT M+K G N LQ  G+F+G Y++A+A+M +ISS D+P LQDYV
Sbjct: 241  RRTNVFMDSGPQIPTNVTPMLKYGTNLLQAVGQFNGHYILAIAFMAVISSPDNPVLQDYV 300

Query: 1087 YPRSSDVSSDCVITE---EISLICPSSGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPT 917
             P  S + SD  I E    ISL CP S  RI +PVKGH CKH QCFD+  F++ NS RP+
Sbjct: 301  QPAVSMLHSDNEIVEGPSRISLNCPISRTRIKVPVKGHSCKHLQCFDFGNFVEINSRRPS 360

Query: 916  WKCPLAGCRKPISNLDIRIDQKFLKILNEVAENVSKVYISDEGSWTV---DPSAIDQPTM 746
            W+CP   C + +   DIRIDQ  +K+L EV ENV+ V IS +GSW         +DQP +
Sbjct: 361  WRCP--HCNQYVCYTDIRIDQNMVKVLKEVGENVADVIISADGSWKAILESNDHVDQPRV 418

Query: 745  TT 740
             T
Sbjct: 419  GT 420


>ref|XP_010661825.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Vitis vinifera]
          Length = 859

 Score =  199 bits (505), Expect = 1e-51
 Identities = 114/267 (42%), Positives = 160/267 (59%), Gaps = 21/267 (7%)
 Frame = -2

Query: 1495 GWFLDEDKDCLLRMADEASLGYFNLDNLDIEPTNG-HTISDIISRFYPSMTVEQILISFA 1319
            GWFLD D   LL +A E    +  +++++ EP     ++S I+SR+YP + +  +L S  
Sbjct: 105  GWFLDHDATDLLTLAKEIGKIFSTMEDINAEPHYPLPSVSKIMSRYYPRLRMGHVLASLE 164

Query: 1318 VKAG--------------EGPPEADFYIAPDTV---PNQNVGKGPQLPSNVTKMVKDGVN 1190
            VK                  PP+ +F +    V    N ++  GPQLP+NV  M++ G+N
Sbjct: 165  VKCLFVAQTDNMDTSSCIVTPPQVNFLLNGKGVWGRINVSMDNGPQLPTNVIAMLRYGIN 224

Query: 1189 NLQVFGEFDGDYVIALAYMNLISSSDSPQLQDYVYPRSSDVSSDCVITE---EISLICPS 1019
             LQV G+F+G+YVI +A+M++IS+S  P+LQ+Y+ P +    SD  I E    ISL CP 
Sbjct: 225  LLQVVGQFNGNYVIIIAFMSVISTSGIPELQEYIQPVAVTSDSDLEIIEGQARISLNCPI 284

Query: 1018 SGCRISMPVKGHLCKHSQCFDYEFFMKANSIRPTWKCPLAGCRKPISNLDIRIDQKFLKI 839
            S  RI++PVKGHLCKH QCFDY  F++ NS RP+W+CP   C + + N DIRIDQ  +KI
Sbjct: 285  SFRRINIPVKGHLCKHHQCFDYGNFIEINSRRPSWRCP--HCNQSVCNPDIRIDQNMVKI 342

Query: 838  LNEVAENVSKVYISDEGSWTVDPSAID 758
            L EV ENV  V IS +GSW     +ID
Sbjct: 343  LREVGENVVDVIISPDGSWKPVVESID 369