BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00018203
         (1455 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_021989008.1| mitotic spindle checkpoint protein MAD1 [Hel...   639   0.0  
ref|XP_023730297.1| mitotic spindle checkpoint protein MAD1 [Lac...   629   0.0  
gb|KVH93535.1| Spindle assembly checkpoint component Mad1 [Cynar...   588   0.0  
gb|PLY76574.1| hypothetical protein LSAT_5X104280 [Lactuca sativa]    579   0.0  
ref|XP_021625944.1| mitotic spindle checkpoint protein MAD1 [Man...   545   0.0  
emb|CDO97655.1| unnamed protein product [Coffea canephora]            545   0.0  
ref|XP_002269253.1| PREDICTED: mitotic spindle checkpoint protei...   543   0.0  
ref|XP_023904822.1| mitotic spindle checkpoint protein MAD1 [Que...   543   0.0  
gb|PIN23581.1| Mitotic checkpoint protein MAD1 [Handroanthus imp...   538   0.0  
ref|XP_021298346.1| mitotic spindle checkpoint protein MAD1 isof...   541   0.0  
ref|XP_021298345.1| mitotic spindle checkpoint protein MAD1 isof...   541   0.0  
ref|XP_008218644.1| PREDICTED: mitotic spindle checkpoint protei...   541   0.0  
ref|XP_012840251.1| PREDICTED: mitotic spindle checkpoint protei...   541   0.0  
gb|KJB67109.1| hypothetical protein B456_010G175200 [Gossypium r...   535   0.0  
ref|XP_009355380.1| PREDICTED: mitotic spindle checkpoint protei...   540   0.0  
ref|XP_008378950.1| PREDICTED: mitotic spindle checkpoint protei...   540   0.0  
ref|XP_007225196.1| mitotic spindle checkpoint protein MAD1 [Pru...   540   0.0  
ref|XP_021639548.1| mitotic spindle checkpoint protein MAD1 isof...   540   0.0  
gb|KJB67110.1| hypothetical protein B456_010G175200 [Gossypium r...   535   0.0  
gb|KJB67108.1| hypothetical protein B456_010G175200 [Gossypium r...   535   0.0  

>ref|XP_021989008.1| mitotic spindle checkpoint protein MAD1 [Helianthus annuus]
 gb|OTG11680.1| putative mitotic checkpoint family protein [Helianthus annuus]
          Length = 715

 Score =  639 bits (1648), Expect = 0.0
 Identities = 342/437 (78%), Positives = 372/437 (85%), Gaps = 2/437 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARKWK+SHEDI                   E+ KLSEA +N KKLEDELSSW+S
Sbjct: 279  ESEVREARKWKASHEDIELLREKLLEEKGCRERAESEVLKLSEAQINGKKLEDELSSWKS 338

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            ++K+IPGVTSAA+IPLKFEAL+KEVLE MMKMGE +ARLKQVEVALD AEN RQN ETE 
Sbjct: 339  IIKDIPGVTSAADIPLKFEALQKEVLEGMMKMGEVTARLKQVEVALDVAENTRQNVETEF 398

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTVKELES 887
            A                   LSS TEERD LK  +DQLK QK VEAG AM+D TVKELE+
Sbjct: 399  ALTNEKMELLKLELKRTELLLSSTTEERDRLKIVVDQLKQQKTVEAGGAMTDGTVKELEA 458

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLAMKED IK+LESSI EQKD+N RQHNEIKLLNERL++ESRRIKSLEREDDRLRSEIAI
Sbjct: 459  SLAMKEDLIKQLESSIQEQKDVNTRQHNEIKLLNERLSNESRRIKSLEREDDRLRSEIAI 518

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESKLGHGDFSSASTKVLRMVNSLAVDS++KQTIEALQNELQKTK++LQAI+ELK+QSGD
Sbjct: 519  LESKLGHGDFSSASTKVLRMVNSLAVDSEAKQTIEALQNELQKTKDKLQAIEELKKQSGD 578

Query: 526  AP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYISGK+VQLKEQIATLEKREERYK VFA+RISVFRRACCELFGYKIVMDDHQR
Sbjct: 579  AGTLVDSYISGKVVQLKEQIATLEKREERYKAVFADRISVFRRACCELFGYKIVMDDHQR 638

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
            SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNT ILGN+YT+Q DIS QVEIF+RK+NSIP
Sbjct: 639  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTKILGNNYTQQPDISHQVEIFIRKLNSIP 698

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLTVESFNKRTLS
Sbjct: 699  AFTANLTVESFNKRTLS 715


>ref|XP_023730297.1| mitotic spindle checkpoint protein MAD1 [Lactuca sativa]
          Length = 715

 Score =  629 bits (1622), Expect = 0.0
 Identities = 340/437 (77%), Positives = 368/437 (84%), Gaps = 2/437 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARKWKSSHEDI                    IS+LSEA +NAKKL+DELS WES
Sbjct: 280  ESEVREARKWKSSHEDIELLREKLLEEKSRRERAELGISELSEAQLNAKKLQDELSCWES 339

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            MMKEIPGVT AA+IP KF AL+K+V+E MMKMGE SARLKQVEVA DAAEN RQ AETES
Sbjct: 340  MMKEIPGVTCAADIPPKFAALQKDVIESMMKMGEVSARLKQVEVAFDAAENGRQTAETES 399

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTVKELES 887
            A                   LSSVTEERD LKT++DQLK QKN E GAA S +T   LES
Sbjct: 400  ALTKEKVELLKLDLKRNELLLSSVTEERDRLKTAVDQLKQQKNAETGAA-SAMTDGTLES 458

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLA+KEDYIKKLESS+ EQKD NARQHNEIKLLNERLN+E RRIKSLEREDDRLRSEIAI
Sbjct: 459  SLAIKEDYIKKLESSVLEQKDANARQHNEIKLLNERLNNELRRIKSLEREDDRLRSEIAI 518

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESK+GHGDFSSASTKVLRMVNSLAVDS++KQTIEALQNELQKTKE+LQA++ELK+QS D
Sbjct: 519  LESKVGHGDFSSASTKVLRMVNSLAVDSEAKQTIEALQNELQKTKEKLQAVEELKKQSAD 578

Query: 526  AP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYISGK VQLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMDDHQR
Sbjct: 579  AGTLVDSYISGKTVQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDDHQR 638

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
            SDGIPVTRFTL SIYAQS+DEKLQFEYESGNT ILGN+YT+QRDIS+QVEIF+ KMNSIP
Sbjct: 639  SDGIPVTRFTLHSIYAQSEDEKLQFEYESGNTKILGNNYTQQRDISKQVEIFIGKMNSIP 698

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLT+ESFNKRTLS
Sbjct: 699  AFTANLTIESFNKRTLS 715


>gb|KVH93535.1| Spindle assembly checkpoint component Mad1 [Cynara cardunculus var.
            scolymus]
          Length = 687

 Score =  588 bits (1517), Expect = 0.0
 Identities = 325/437 (74%), Positives = 347/437 (79%), Gaps = 2/437 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARKWKSSHEDI                   EISKLSE  VN K LEDELSSWES
Sbjct: 284  ESEVREARKWKSSHEDIEVLREKLLEEKGRRERAESEISKLSEEQVNGKMLEDELSSWES 343

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            MMKEIPGVT AAEIP KF AL++EV+E MMK+GE +ARLKQV VALDAAEN RQNAETE+
Sbjct: 344  MMKEIPGVTCAAEIPFKFAALQREVIESMMKIGEVTARLKQVXVALDAAENGRQNAETEA 403

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTVKELES 887
            A                   LSSVTEERD LK                            
Sbjct: 404  ALTKEKVELSKLELKRTELLLSSVTEERDRLK---------------------------- 435

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
                 EDYIKKLESSI EQK++NARQHNEIK+LNERLNSESRRIKSLEREDDRLRSEIAI
Sbjct: 436  -----EDYIKKLESSILEQKEINARQHNEIKVLNERLNSESRRIKSLEREDDRLRSEIAI 490

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESK+GHGDFSSASTKVLRMVNSLAVDS++KQTIEALQNELQKTKE+LQAI+ELK+QSGD
Sbjct: 491  LESKVGHGDFSSASTKVLRMVNSLAVDSEAKQTIEALQNELQKTKEKLQAIEELKKQSGD 550

Query: 526  AP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYISGKIVQLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMDDHQR
Sbjct: 551  AGTLVDSYISGKIVQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDDHQR 610

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
            SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNT ILGN+YT+Q +IS QVEIF+RK+NSIP
Sbjct: 611  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTKILGNNYTQQPEISHQVEIFIRKLNSIP 670

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLTVESFNKRTLS
Sbjct: 671  AFTANLTVESFNKRTLS 687


>gb|PLY76574.1| hypothetical protein LSAT_5X104280 [Lactuca sativa]
          Length = 693

 Score =  579 bits (1493), Expect = 0.0
 Identities = 318/435 (73%), Positives = 348/435 (80%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARKWKSSHEDI                    IS+LSEA +NAKKL+DELS WES
Sbjct: 280  ESEVREARKWKSSHEDIELLREKLLEEKSRRERAELGISELSEAQLNAKKLQDELSCWES 339

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            MMKEIPGVT AA+IP KF AL+K+V+E MMKMGE SARLKQVEVA DAAEN RQ AETES
Sbjct: 340  MMKEIPGVTCAADIPPKFAALQKDVIESMMKMGEVSARLKQVEVAFDAAENGRQTAETES 399

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTVKELES 887
            A                   LSSVTEERD LKT++DQLK QKN E GAA S +T   LES
Sbjct: 400  ALTKEKVELLKLDLKRNELLLSSVTEERDRLKTAVDQLKQQKNAETGAA-SAMTDGTLES 458

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLA+KEDYIKKLESS+ EQKD NARQHNEIKLLNERLN+E RRIKSLEREDDRLRSEIAI
Sbjct: 459  SLAIKEDYIKKLESSVLEQKDANARQHNEIKLLNERLNNELRRIKSLEREDDRLRSEIAI 518

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESK+GHGDFSSASTKVLRMVNSLAVDS++KQTIEALQNELQKTKE+LQA++ELK+QS D
Sbjct: 519  LESKVGHGDFSSASTKVLRMVNSLAVDSEAKQTIEALQNELQKTKEKLQAVEELKKQSAD 578

Query: 526  APVDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQRSD 347
            A       G +V             + YKTVFA+RISVFRRACCELFGYKIVMDDHQRSD
Sbjct: 579  A-------GTLV-------------DSYKTVFADRISVFRRACCELFGYKIVMDDHQRSD 618

Query: 346  GIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIPAF 167
            GIPVTRFTL SIYAQS+DEKLQFEYESGNT ILGN+YT+QRDIS+QVEIF+ KMNSIPAF
Sbjct: 619  GIPVTRFTLHSIYAQSEDEKLQFEYESGNTKILGNNYTQQRDISKQVEIFIGKMNSIPAF 678

Query: 166  TANLTVESFNKRTLS 122
            TANLT+ESFNKRTLS
Sbjct: 679  TANLTIESFNKRTLS 693


>ref|XP_021625944.1| mitotic spindle checkpoint protein MAD1 [Manihot esculenta]
 ref|XP_021625946.1| mitotic spindle checkpoint protein MAD1 [Manihot esculenta]
 ref|XP_021625947.1| mitotic spindle checkpoint protein MAD1 [Manihot esculenta]
 gb|OAY39587.1| hypothetical protein MANES_10G106700 [Manihot esculenta]
          Length = 722

 Score =  545 bits (1403), Expect = 0.0
 Identities = 292/438 (66%), Positives = 350/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK K+S+E+                    E+SKL E  +N +KLEDELSSW+S
Sbjct: 285  ESEVREARKLKASYENTELLKEKLLEEKSRKERAESELSKLREFELNTRKLEDELSSWKS 344

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            M+K+IP V+   +IPLKF AL+KEV++ MMK+GE +ARLKQ+EVALDAA+  +QNAE E+
Sbjct: 345  MLKKIPDVSCCDDIPLKFSALQKEVIDNMMKVGEANARLKQIEVALDAAQLGKQNAEAEA 404

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVT-VKELE 890
            A                   LS+VTEERD LK   D+L+  KN +AG  +S  T ++ELE
Sbjct: 405  ALAKEKAESLKLEVNRIQLMLSAVTEERDRLKNIADELRRSKNEQAGDGVSSGTHLQELE 464

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSL  K+  IK+LES++ EQK++N RQ  EI LLN+RLN+E+RRIKSLERE DRLRSEI+
Sbjct: 465  SSLMKKDCLIKELESNLNEQKEVNHRQLAEINLLNDRLNNEARRIKSLERESDRLRSEIS 524

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+A+TKVLRMVN+LAVD+D+KQTIEAL  ELQKTKE+LQA++ELK QSG
Sbjct: 525  LLESKLGHGDYSAANTKVLRMVNTLAVDNDAKQTIEALHTELQKTKEKLQAVEELKSQSG 584

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            DA   VDSYISGK+ QLKEQIATLEKREERY+TVFAERISVFRRACCELFGYKIVMD+HQ
Sbjct: 585  DAGKLVDSYISGKVTQLKEQIATLEKREERYRTVFAERISVFRRACCELFGYKIVMDEHQ 644

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            RS+GIPVTRFTLQSIYAQSDDEKL+FEYESGNTNIL N YT Q +IS+QV+IF+ KMNSI
Sbjct: 645  RSNGIPVTRFTLQSIYAQSDDEKLEFEYESGNTNILANAYTSQSEISRQVDIFIHKMNSI 704

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFN+RTLS
Sbjct: 705  PAFTANLTVESFNRRTLS 722


>emb|CDO97655.1| unnamed protein product [Coffea canephora]
          Length = 728

 Score =  545 bits (1403), Expect = 0.0
 Identities = 287/438 (65%), Positives = 349/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSSHE++                   ++ KL E  +  +KL+DELS+W+S
Sbjct: 291  ESEVREARKLKSSHENVELLKEKLQEEKRRRERAELDLLKLPEIQLRIEKLQDELSAWKS 350

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            +MK+IPGV+ A ++P+KF AL+KEV++ MMK+GE   RLK++EVALDAAE  +QNA TE+
Sbjct: 351  LMKDIPGVSCADDVPMKFAALQKEVIDSMMKVGEAQVRLKEIEVALDAAELDKQNAITEA 410

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAM-SDVTVKELE 890
                                L+S  EE+D LK   ++LK +K+ + G  + S   V+ELE
Sbjct: 411  TLAKEKAESSKSEVKRIELMLASALEEKDRLKNMFNELKREKHAQGGPEVASGALVQELE 470

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            +S+A KE+YIK+LES++ EQ  +N  Q NEIKLL+ERL  E+RRIKSLERE DRLRSE+A
Sbjct: 471  ASIAKKENYIKELESNLHEQNLINIHQQNEIKLLSERLTDEARRIKSLEREGDRLRSELA 530

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGDFSSA+TKVLRMVN+LAVD+++KQTIEALQNELQKTKE+LQA++ELK+QS 
Sbjct: 531  LLESKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIEALQNELQKTKEKLQAVEELKKQSA 590

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            DA   VDSY+SGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ
Sbjct: 591  DAGTLVDSYVSGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 650

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            R DGIPVTRFTLQSIYAQ+DDE+L+FEYESGNTNI+ NDYT+Q +IS QVEIF+RKMNSI
Sbjct: 651  RPDGIPVTRFTLQSIYAQADDERLEFEYESGNTNIMVNDYTRQPEISHQVEIFMRKMNSI 710

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLT+ESFNKRTLS
Sbjct: 711  PAFTANLTMESFNKRTLS 728


>ref|XP_002269253.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Vitis vinifera]
          Length = 717

 Score =  543 bits (1400), Expect = 0.0
 Identities = 290/435 (66%), Positives = 351/435 (80%), Gaps = 3/435 (0%)
 Frame = -3

Query: 1420 EVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWESMM 1241
            EVREARK KSSHE+I                   E+ KL E  ++ KKLEDEL SW+ M+
Sbjct: 282  EVREARKLKSSHENIELLKEKLLEEKGRRERAESELLKLPEIQLSMKKLEDELLSWKLMV 341

Query: 1240 KEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETESAX 1061
            K+IPGV+ + ++P+KF AL+KEV+E MMK+GE +ARLKQ+EV+LDAAE+A+QNAETE+A 
Sbjct: 342  KDIPGVSCSDDVPMKFAALQKEVIEGMMKLGEANARLKQMEVSLDAAEHAKQNAETEAAS 401

Query: 1060 XXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTV-KELESS 884
                              L  VTEERD L+ ++++LK QKNVEAG    + T+ +E E S
Sbjct: 402  AKESSEVSKSEVKRIELMLGLVTEERDQLRNAINELKKQKNVEAGDETKNGTLLQEFELS 461

Query: 883  LAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAIL 704
            LA KE+ IK+LE+++ EQK++N R+ NEIKLLNE+LN+E+RRIKSLERE DRLRSEI++L
Sbjct: 462  LAKKENCIKELENNLCEQKEVNNRRFNEIKLLNEKLNNEARRIKSLEREGDRLRSEISLL 521

Query: 703  ESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGDA 524
            ESKLGHGDFS+ +TKVLRMVN+LAVD+++KQTIEALQ ELQK KE+L+AI+ELK QS D+
Sbjct: 522  ESKLGHGDFSATNTKVLRMVNTLAVDNEAKQTIEALQTELQKAKEKLEAIEELKTQSADS 581

Query: 523  P--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQRS 350
               VDSY++GKIVQ KEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMDDHQR 
Sbjct: 582  GKLVDSYVAGKIVQFKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDDHQRP 641

Query: 349  DGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIPA 170
            +GIPVTRFTLQSIYAQSDDEKL+FEYESGNTNIL N YT Q +ISQQVEIF++K+NSIPA
Sbjct: 642  NGIPVTRFTLQSIYAQSDDEKLEFEYESGNTNILANAYTSQPEISQQVEIFIQKLNSIPA 701

Query: 169  FTANLTVESFNKRTL 125
            FTANLTVESFNKRTL
Sbjct: 702  FTANLTVESFNKRTL 716


>ref|XP_023904822.1| mitotic spindle checkpoint protein MAD1 [Quercus suber]
 gb|POF20167.1| mitotic spindle checkpoint protein mad1 [Quercus suber]
          Length = 723

 Score =  543 bits (1400), Expect = 0.0
 Identities = 293/438 (66%), Positives = 351/438 (80%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            ++EVREARK +SSHE+I                   E+ KL E  ++ KKLEDELSSW+ 
Sbjct: 286  EAEVREARKLRSSHENIELLKEKLLEEKGRRERAEAELPKLQEFQMSMKKLEDELSSWKL 345

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            M  EIPGV+ + +IP+KF AL+KEV++  MK+G+ +ARLKQ+EVALDAA + +QNAETE+
Sbjct: 346  MTSEIPGVSCSEDIPVKFAALQKEVIDSTMKVGDANARLKQLEVALDAALSEKQNAETEA 405

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVT-VKELE 890
            A                   LS VTEERD L+  L++ K  KN EAG  ++  T V+ELE
Sbjct: 406  ALVKEKAAVSKSEVKRIELMLSMVTEERDKLRNLLNEFKRMKNDEAGDEVASGTLVQELE 465

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE  IK++ESS+  QK++N RQ+ EIKLLNE+LN+E+R+IKSLERE DRLRSEIA
Sbjct: 466  SSLAKKEFCIKEMESSLHVQKEVNTRQYEEIKLLNEKLNNEARKIKSLEREGDRLRSEIA 525

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+A+TKVLRMVN+LAVD+D+KQTIEAL+ ELQKTKE+LQA++ELK QSG
Sbjct: 526  LLESKLGHGDYSAANTKVLRMVNTLAVDNDAKQTIEALRTELQKTKEKLQAVEELKSQSG 585

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            DA   VDSYISGKI QLKEQIATLEKREERYKTVFA+RISVFRRACCELFG+KIVMD+HQ
Sbjct: 586  DAGTLVDSYISGKITQLKEQIATLEKREERYKTVFADRISVFRRACCELFGFKIVMDEHQ 645

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            RS+GIPVT FTLQSIYAQSDDEKL+FEYESGNT IL NDYT Q +IS+QVEIF+RKMNSI
Sbjct: 646  RSNGIPVTHFTLQSIYAQSDDEKLEFEYESGNTTILANDYTSQPEISRQVEIFIRKMNSI 705

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFN+RTLS
Sbjct: 706  PAFTANLTVESFNRRTLS 723


>gb|PIN23581.1| Mitotic checkpoint protein MAD1 [Handroanthus impetiginosus]
          Length = 599

 Score =  538 bits (1385), Expect = 0.0
 Identities = 284/440 (64%), Positives = 351/440 (79%), Gaps = 3/440 (0%)
 Frame = -3

Query: 1432 YQQSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSW 1253
            + +SEVREARK K+SHEDI                   ++ KLS+  +  KK EDELS+W
Sbjct: 160  HYESEVREARKIKASHEDIQLLKEKLYEEKSRRERAELDLLKLSDLQLTLKKFEDELSTW 219

Query: 1252 ESMMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAET 1073
            +S++KEIPGV+SA +I  KF AL+KEV++ MM++GE  AR+K+++VALD A   +QNAET
Sbjct: 220  KSLIKEIPGVSSADDILPKFAALQKEVVDSMMRVGEAQARVKEIQVALDTAMLDKQNAET 279

Query: 1072 ESAXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGA-AMSDVTVKE 896
            E+A                   L  +TEERD LK  + +LK + N+E G   +S   V+E
Sbjct: 280  ETAMAKEKAESHKAEIKRLELMLGLITEERDCLKNVVKELKERSNLEGGTEVVSGTIVQE 339

Query: 895  LESSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSE 716
            LE+SLA KE+Y+++LESS+ E+++ ++RQHNEIKLLNERL +E+RRIK LERE DRLRSE
Sbjct: 340  LEASLAKKENYVRELESSLLEKRETSSRQHNEIKLLNERLTNEARRIKMLERESDRLRSE 399

Query: 715  IAILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQ 536
            I++LESKLGHGDFSSA+TKVLRMVN+LAVD+++KQTIEALQ ELQKTKE+LQA++ELK+Q
Sbjct: 400  ISLLESKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIEALQTELQKTKEKLQAVEELKKQ 459

Query: 535  SGDA--PVDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDD 362
            S DA  PVDSY++GKI QLKEQIATLEKREERYK+VFAE+ISVFRRACCELFGYKIVMDD
Sbjct: 460  SSDAGAPVDSYVAGKIKQLKEQIATLEKREERYKSVFAEKISVFRRACCELFGYKIVMDD 519

Query: 361  HQRSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMN 182
            HQR DGIPVTRFTL SIYA SDDEKLQFEYESGNTNI+ N+YT Q +IS+Q++IF+RKM+
Sbjct: 520  HQRPDGIPVTRFTLHSIYALSDDEKLQFEYESGNTNIVVNEYTSQPEISRQIDIFIRKMS 579

Query: 181  SIPAFTANLTVESFNKRTLS 122
            SIPAFTANLTVESFNKRTLS
Sbjct: 580  SIPAFTANLTVESFNKRTLS 599


>ref|XP_021298346.1| mitotic spindle checkpoint protein MAD1 isoform X2 [Herrania
            umbratica]
          Length = 723

 Score =  541 bits (1395), Expect = 0.0
 Identities = 291/438 (66%), Positives = 349/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSSHE+I                   E+SKL E  ++  KLEDELSSW+ 
Sbjct: 286  ESEVREARKLKSSHENIELLKAKLLGEKGRRERAESELSKLQELQISFNKLEDELSSWKL 345

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            MMK+IPGV+   +IP+KF  L+KEV++  +K+G+ +ARLKQ+EV LDAA+ A+Q+AET++
Sbjct: 346  MMKDIPGVSCPDDIPVKFATLQKEVIDSTIKIGDANARLKQIEVGLDAAQLAKQDAETDA 405

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAM-SDVTVKELE 890
                                LS VTEERD L+  +D+LK  KN EAG  + S   V+ELE
Sbjct: 406  VLAKEKAEVLKSEVKRIELMLSVVTEERDKLRNVIDELKRPKNEEAGDQVASGNVVQELE 465

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE  IK+L+S++ EQK++N RQHNEIKLL++RLN+E+RRIKSLERE DRLRSEI+
Sbjct: 466  SSLAKKESCIKELQSNLHEQKEVNDRQHNEIKLLHDRLNNEARRIKSLERESDRLRSEIS 525

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+A+TKVLRMVN+LAVD+++KQTIEALQ ELQKTKE+LQA++ELK QSG
Sbjct: 526  LLESKLGHGDYSAANTKVLRMVNTLAVDNEAKQTIEALQTELQKTKEKLQALEELKSQSG 585

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            D    VDSYIS KI++LKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQ
Sbjct: 586  DTGKLVDSYISEKIMKLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQ 645

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            R +GIPVT FTLQSIYAQSDDEKL+FEYESGNTNIL NDYT   +IS QV+IFVRKMNSI
Sbjct: 646  RPNGIPVTHFTLQSIYAQSDDEKLEFEYESGNTNILANDYTAHPEISHQVDIFVRKMNSI 705

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFNKRTLS
Sbjct: 706  PAFTANLTVESFNKRTLS 723


>ref|XP_021298345.1| mitotic spindle checkpoint protein MAD1 isoform X1 [Herrania
            umbratica]
          Length = 724

 Score =  541 bits (1395), Expect = 0.0
 Identities = 291/438 (66%), Positives = 349/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSSHE+I                   E+SKL E  ++  KLEDELSSW+ 
Sbjct: 287  ESEVREARKLKSSHENIELLKAKLLGEKGRRERAESELSKLQELQISFNKLEDELSSWKL 346

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            MMK+IPGV+   +IP+KF  L+KEV++  +K+G+ +ARLKQ+EV LDAA+ A+Q+AET++
Sbjct: 347  MMKDIPGVSCPDDIPVKFATLQKEVIDSTIKIGDANARLKQIEVGLDAAQLAKQDAETDA 406

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAM-SDVTVKELE 890
                                LS VTEERD L+  +D+LK  KN EAG  + S   V+ELE
Sbjct: 407  VLAKEKAEVLKSEVKRIELMLSVVTEERDKLRNVIDELKRPKNEEAGDQVASGNVVQELE 466

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE  IK+L+S++ EQK++N RQHNEIKLL++RLN+E+RRIKSLERE DRLRSEI+
Sbjct: 467  SSLAKKESCIKELQSNLHEQKEVNDRQHNEIKLLHDRLNNEARRIKSLERESDRLRSEIS 526

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+A+TKVLRMVN+LAVD+++KQTIEALQ ELQKTKE+LQA++ELK QSG
Sbjct: 527  LLESKLGHGDYSAANTKVLRMVNTLAVDNEAKQTIEALQTELQKTKEKLQALEELKSQSG 586

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            D    VDSYIS KI++LKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQ
Sbjct: 587  DTGKLVDSYISEKIMKLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQ 646

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            R +GIPVT FTLQSIYAQSDDEKL+FEYESGNTNIL NDYT   +IS QV+IFVRKMNSI
Sbjct: 647  RPNGIPVTHFTLQSIYAQSDDEKLEFEYESGNTNILANDYTAHPEISHQVDIFVRKMNSI 706

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFNKRTLS
Sbjct: 707  PAFTANLTVESFNKRTLS 724


>ref|XP_008218644.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Prunus mume]
          Length = 717

 Score =  541 bits (1394), Expect = 0.0
 Identities = 292/437 (66%), Positives = 350/437 (80%), Gaps = 3/437 (0%)
 Frame = -3

Query: 1423 SEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWESM 1244
            +EVREARK KSSHE++                   E+SKL E   + K LEDEL+SW+ M
Sbjct: 281  AEVREARKLKSSHENVELLKEKLLEEKSRRERVESELSKLQELQPSMKTLEDELTSWKLM 340

Query: 1243 MKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETESA 1064
            +K+IPGV+ + +IP+KF AL+KEV++ MMK+G  +ARLKQVEV+LDAA+  +QNAETE+A
Sbjct: 341  LKDIPGVSCSEDIPVKFAALQKEVIDSMMKVGLANARLKQVEVSLDAAQIDKQNAETEAA 400

Query: 1063 XXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVT-VKELES 887
                               LS VTEERD L+  +++LKL KN EAG   S+ T ++ELES
Sbjct: 401  LAKEKVDASKSEVNRIELMLSMVTEERDKLRNVVNELKLAKNDEAGHETSNQTFLQELES 460

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLA KE YIK+LE  + EQK++N+RQ  EIKLLNERLN+E+RRIKSLERE DRL SEIA+
Sbjct: 461  SLAKKECYIKELECGLCEQKEVNSRQREEIKLLNERLNNEARRIKSLERESDRLGSEIAL 520

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESKLGHGDFS+A+TKVLRMVN+L VD+++KQTIEAL+ ELQKTKE+LQA++EL+ QSGD
Sbjct: 521  LESKLGHGDFSAANTKVLRMVNTLTVDNEAKQTIEALRTELQKTKEKLQAVEELRSQSGD 580

Query: 526  AP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYIS KIVQLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQR
Sbjct: 581  AGKLVDSYISEKIVQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 640

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
             +GIPVTRFTLQSIYAQSDDEKL+FEYESG+TNIL NDYT   +IS QVEIF+RK+NSIP
Sbjct: 641  PNGIPVTRFTLQSIYAQSDDEKLEFEYESGSTNILANDYTSHPEISHQVEIFIRKLNSIP 700

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLTVESFN+RTL+
Sbjct: 701  AFTANLTVESFNRRTLT 717


>ref|XP_012840251.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Erythranthe
            guttata]
          Length = 731

 Score =  541 bits (1394), Expect = 0.0
 Identities = 287/438 (65%), Positives = 345/438 (78%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK K+ HEDI                   ++ KLS+  +N   L DELS W+S
Sbjct: 294  ESEVREARKIKAYHEDIELLKEKLYEEKRRRERAELDLVKLSDLQLNVNTLNDELSIWKS 353

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            ++KEIPGV SA +IP KF AL++EV + M + GE  A +K+++VALD A   +Q AETE+
Sbjct: 354  VVKEIPGVLSADDIPSKFAALQREVADSMRRAGEAQACMKEIQVALDTAMLDKQTAETEA 413

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVT-VKELE 890
            A                      +TEERD L+  + +LK +KNVE G  ++  T V+ELE
Sbjct: 414  ALAKEKAESSEAEIKRLELMFGLITEERDRLQNLVKELKERKNVEGGTELAGGTIVQELE 473

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE+Y+++LE+S+ EQK++N+RQHNEIKLLNERL +E+RR+K LER+ DRLRSEI+
Sbjct: 474  SSLAKKENYVRELENSLSEQKEINSRQHNEIKLLNERLTNEARRLKMLERDGDRLRSEIS 533

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGDFSSA+TKVLRMVN+LAVD+++KQTIEALQNELQKTKEQLQA+DELK+QS 
Sbjct: 534  LLESKLGHGDFSSANTKVLRMVNTLAVDNEAKQTIEALQNELQKTKEQLQAVDELKKQSS 593

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            DA   VDSYI+GKI QLKEQIA LEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ
Sbjct: 594  DAGAHVDSYIAGKIKQLKEQIAKLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 653

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            RSDGIPVTRFTL SIYA SDDEKLQF+YESGNTNI+ N+YT QR+IS+QV+IF+RKMNSI
Sbjct: 654  RSDGIPVTRFTLNSIYALSDDEKLQFDYESGNTNIVVNEYTSQREISRQVDIFIRKMNSI 713

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFNKRTLS
Sbjct: 714  PAFTANLTVESFNKRTLS 731


>gb|KJB67109.1| hypothetical protein B456_010G175200 [Gossypium raimondii]
          Length = 566

 Score =  535 bits (1378), Expect = 0.0
 Identities = 286/438 (65%), Positives = 347/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSS+EDI                   E+SKL E  ++   LE+ELS W+ 
Sbjct: 129  ESEVREARKLKSSYEDIELLKAKLLEEKGRRERAESELSKLQEQQISLSNLENELSLWKL 188

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            +MK+IPGV+   +IP+KF  L+KEV+E  MK+GET+ARLKQ+EV LDAA  A+QNAE E+
Sbjct: 189  IMKDIPGVSCPEDIPVKFATLQKEVIESTMKIGETNARLKQIEVGLDAAHLAKQNAEAEA 248

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTV-KELE 890
            A                   LS VTEER+ L  ++++LK  KN EAG  ++  TV +ELE
Sbjct: 249  ALDKEKAEILKSEVKRIELMLSMVTEERNKLSNAINELKRHKNEEAGDEVASGTVVQELE 308

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE  IK+LE S+ EQK++N RQHNEIK+L++RLN+E+RRIKSL+RE DRLRSEI+
Sbjct: 309  SSLAKKESCIKELERSLHEQKEINDRQHNEIKMLHDRLNNEARRIKSLDRESDRLRSEIS 368

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+++TKVLRMVN+LAVD+++KQTIEALQ ELQKTKE++QA++ELK QSG
Sbjct: 369  LLESKLGHGDYSASNTKVLRMVNTLAVDNEAKQTIEALQTELQKTKEKVQALEELKSQSG 428

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            D    VDSYIS KI +LKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQ
Sbjct: 429  DTGKLVDSYISEKITKLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQ 488

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            R +GIPVT FTLQSIYAQ+DDEKL+FEYESGNTNIL NDYT Q +IS QV+IFVRK+NSI
Sbjct: 489  RPNGIPVTHFTLQSIYAQNDDEKLEFEYESGNTNILANDYTAQPEISHQVDIFVRKLNSI 548

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFN+RTLS
Sbjct: 549  PAFTANLTVESFNRRTLS 566


>ref|XP_009355380.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Pyrus x
            bretschneideri]
          Length = 724

 Score =  540 bits (1392), Expect = 0.0
 Identities = 289/437 (66%), Positives = 349/437 (79%), Gaps = 3/437 (0%)
 Frame = -3

Query: 1423 SEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWESM 1244
            +EVREARK K +HE++                   E+SKL E  ++ KKLEDEL+SW+ +
Sbjct: 288  AEVREARKLKLAHENVELLKEKLLEEKGRRERAEAELSKLQELQLSMKKLEDELTSWKLI 347

Query: 1243 MKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETESA 1064
             ++IPGV+ + ++P+KF AL+KEV++ MMK+GE +A LKQ+EVALDAA+  +QNAE E+ 
Sbjct: 348  SQDIPGVSCSEDVPVKFAALQKEVIDSMMKVGEANAALKQLEVALDAAKIDKQNAEIEAT 407

Query: 1063 XXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAG-AAMSDVTVKELES 887
                               LS VTEERD L+  L+ LK  KN EAG  A S   ++E+ES
Sbjct: 408  LAKEKVDVSKSEVKQIELMLSMVTEERDKLRNVLNGLKSAKNDEAGDEAASQTFLQEVES 467

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLA KE YIK+LE  + EQK++N+RQ  EIKLLNERLN+++RRIKSLERE DRLRSEIA+
Sbjct: 468  SLATKEAYIKELECGLCEQKEVNSRQREEIKLLNERLNNDARRIKSLERESDRLRSEIAL 527

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESK+GHGDFS+A+TKVLRMVN+L VD+++KQTIEALQ ELQKTKE+LQA++ELK QSGD
Sbjct: 528  LESKIGHGDFSAANTKVLRMVNTLTVDNEAKQTIEALQTELQKTKEKLQAVEELKSQSGD 587

Query: 526  AP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYISGKIVQLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQR
Sbjct: 588  AGTLVDSYISGKIVQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 647

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
             +GIPVTRFTLQSIYAQSDDEKL+FEYESG+TNIL NDYT Q +ISQQVEIF+RK+NSIP
Sbjct: 648  PNGIPVTRFTLQSIYAQSDDEKLEFEYESGSTNILANDYTSQSEISQQVEIFIRKLNSIP 707

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLTVESFN+RTL+
Sbjct: 708  AFTANLTVESFNRRTLT 724


>ref|XP_008378950.1| PREDICTED: mitotic spindle checkpoint protein MAD1 [Malus domestica]
          Length = 724

 Score =  540 bits (1392), Expect = 0.0
 Identities = 289/437 (66%), Positives = 349/437 (79%), Gaps = 3/437 (0%)
 Frame = -3

Query: 1423 SEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWESM 1244
            +EVREARK K +HE++                   E+SKL E  ++ KKLE+EL++W+ M
Sbjct: 288  AEVREARKLKLAHENVELLKEKLLEEKGRRERAEAELSKLQELQLSMKKLENELTTWKLM 347

Query: 1243 MKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETESA 1064
             K+IPGV+ + +IP+KF AL+KEV++ MMK+GE +A LKQ+EVALDAA+  +QNAE E+ 
Sbjct: 348  SKDIPGVSCSEDIPVKFAALQKEVIDSMMKVGEANAALKQLEVALDAAKIDKQNAEIEAN 407

Query: 1063 XXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAG-AAMSDVTVKELES 887
                               LS VTEERD L+  L+ LK  KN EAG  A S   ++E+ES
Sbjct: 408  LAKEKVDVSKSEVKRIELMLSMVTEERDKLRNVLNGLKXXKNDEAGDEAASQTFLQEVES 467

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLA KE YIK+LE  + EQK++N+RQ  EIKLLNERLN+E+RR+KSLERE DRLRSEIA+
Sbjct: 468  SLAKKEAYIKELECGLCEQKEVNSRQREEIKLLNERLNNEARRMKSLERESDRLRSEIAL 527

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESK+GHGDFS+A+TKVLRMVN+LA D+++KQTIEALQ ELQKTKE+LQA++ELK QSGD
Sbjct: 528  LESKIGHGDFSAANTKVLRMVNTLAADNEAKQTIEALQTELQKTKEKLQAVEELKSQSGD 587

Query: 526  A--PVDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYISGKIVQLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQR
Sbjct: 588  AGTXVDSYISGKIVQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 647

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
             +GIP+TRFTLQSIYAQSDDEKL+FEYESG+TNIL NDYT Q +ISQQVEIF+RK+NSIP
Sbjct: 648  PNGIPITRFTLQSIYAQSDDEKLEFEYESGSTNILANDYTSQSEISQQVEIFIRKLNSIP 707

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLTVESFN+RTL+
Sbjct: 708  AFTANLTVESFNRRTLT 724


>ref|XP_007225196.1| mitotic spindle checkpoint protein MAD1 [Prunus persica]
 gb|ONI36438.1| hypothetical protein PRUPE_1G585300 [Prunus persica]
          Length = 717

 Score =  540 bits (1390), Expect = 0.0
 Identities = 291/437 (66%), Positives = 349/437 (79%), Gaps = 3/437 (0%)
 Frame = -3

Query: 1423 SEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWESM 1244
            +EVREARK KSSHE++                   E+SKL E   + K LEDEL+SW+ M
Sbjct: 281  AEVREARKLKSSHENVELLKEKLLEEKSRRERVESELSKLQELQPSMKTLEDELTSWKLM 340

Query: 1243 MKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETESA 1064
            +K+IPGV+ + +IP+KF AL+KEV++ MMK+G  +ARLKQVEV+LDAA+  +QNAETE+A
Sbjct: 341  LKDIPGVSCSEDIPVKFAALQKEVIDSMMKVGLANARLKQVEVSLDAAQIDKQNAETEAA 400

Query: 1063 XXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVT-VKELES 887
                               LS VTEERD L+  +++LKL KN EAG   S+ T ++ELES
Sbjct: 401  LAKEKVDASKSEVNRIELMLSMVTEERDKLRNVVNELKLAKNDEAGHETSNQTFLQELES 460

Query: 886  SLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIAI 707
            SLA KE YIK+LE  + EQK++N+RQ  EIKLLNERLN+E+RRIKSLERE DRL SEIA+
Sbjct: 461  SLAKKECYIKELECGLCEQKEVNSRQREEIKLLNERLNNEARRIKSLERESDRLGSEIAL 520

Query: 706  LESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSGD 527
            LESKLGHGDFS+ +TKVLRMVN+L VD+++KQTIEAL+ ELQKTKE+LQA++EL+ QSGD
Sbjct: 521  LESKLGHGDFSAVNTKVLRMVNTLTVDNEAKQTIEALRTELQKTKEKLQAVEELRSQSGD 580

Query: 526  AP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQR 353
            A   VDSYIS KIVQLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQR
Sbjct: 581  AGKLVDSYISEKIVQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR 640

Query: 352  SDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSIP 173
             +GIPVTRFTLQSIYAQSDDEKL+FEYESG+TNIL NDYT   +IS QVEIF+RK+NSIP
Sbjct: 641  PNGIPVTRFTLQSIYAQSDDEKLEFEYESGSTNILANDYTSHPEISHQVEIFIRKLNSIP 700

Query: 172  AFTANLTVESFNKRTLS 122
            AFTANLTVESFN+RTL+
Sbjct: 701  AFTANLTVESFNRRTLT 717


>ref|XP_021639548.1| mitotic spindle checkpoint protein MAD1 isoform X1 [Hevea
            brasiliensis]
 ref|XP_021639549.1| mitotic spindle checkpoint protein MAD1 isoform X1 [Hevea
            brasiliensis]
 ref|XP_021639550.1| mitotic spindle checkpoint protein MAD1 isoform X2 [Hevea
            brasiliensis]
 ref|XP_021639551.1| mitotic spindle checkpoint protein MAD1 isoform X1 [Hevea
            brasiliensis]
          Length = 722

 Score =  540 bits (1390), Expect = 0.0
 Identities = 289/438 (65%), Positives = 352/438 (80%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSS+E+I                   E+SKL E  +N +KLEDELSSW+ 
Sbjct: 285  ESEVREARKLKSSYENIELLKEKLLEEKSRKETAESELSKLRECELNMRKLEDELSSWKL 344

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            M+K+IP V+   +IPLKF AL+KEV++ M+K+GE +ARLKQ+EVALDAA+  +QNAETE+
Sbjct: 345  MIKDIPDVSCCDDIPLKFSALQKEVIDNMIKVGEANARLKQMEVALDAAQLGKQNAETET 404

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAG-AAMSDVTVKELE 890
            A                   LS+VTEERD LK  +D+L+  KN +AG  A S   ++ELE
Sbjct: 405  ALAKEKAEGLKLEVNQIELMLSAVTEERDRLKNIVDELRKSKNEQAGDGAASGSLLQELE 464

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSL  ++  IK+LE ++ E+K++N RQ +EIKLLN+RLN+E+RRIKSLERE DRLRSEI+
Sbjct: 465  SSLMKRDCLIKELERNLHEKKEVNNRQLDEIKLLNDRLNNEARRIKSLERESDRLRSEIS 524

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+A+TKVLRMVN+LAVD+D+KQTIEAL+ ELQKTKE+LQA++ELK QSG
Sbjct: 525  LLESKLGHGDYSAANTKVLRMVNTLAVDNDAKQTIEALRTELQKTKEKLQAVEELKSQSG 584

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            DA   VDSYISGK+ QLKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQ
Sbjct: 585  DAGKLVDSYISGKMTQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQ 644

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            RS+GIPVT FTLQSIYAQ+DDEKL+FEYESGNTNIL N YT Q +IS+QV+IF+ KMNSI
Sbjct: 645  RSNGIPVTHFTLQSIYAQNDDEKLEFEYESGNTNILANAYTSQSEISRQVDIFIHKMNSI 704

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFN+RTLS
Sbjct: 705  PAFTANLTVESFNRRTLS 722


>gb|KJB67110.1| hypothetical protein B456_010G175200 [Gossypium raimondii]
          Length = 602

 Score =  535 bits (1378), Expect = 0.0
 Identities = 286/438 (65%), Positives = 347/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSS+EDI                   E+SKL E  ++   LE+ELS W+ 
Sbjct: 165  ESEVREARKLKSSYEDIELLKAKLLEEKGRRERAESELSKLQEQQISLSNLENELSLWKL 224

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            +MK+IPGV+   +IP+KF  L+KEV+E  MK+GET+ARLKQ+EV LDAA  A+QNAE E+
Sbjct: 225  IMKDIPGVSCPEDIPVKFATLQKEVIESTMKIGETNARLKQIEVGLDAAHLAKQNAEAEA 284

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTV-KELE 890
            A                   LS VTEER+ L  ++++LK  KN EAG  ++  TV +ELE
Sbjct: 285  ALDKEKAEILKSEVKRIELMLSMVTEERNKLSNAINELKRHKNEEAGDEVASGTVVQELE 344

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE  IK+LE S+ EQK++N RQHNEIK+L++RLN+E+RRIKSL+RE DRLRSEI+
Sbjct: 345  SSLAKKESCIKELERSLHEQKEINDRQHNEIKMLHDRLNNEARRIKSLDRESDRLRSEIS 404

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+++TKVLRMVN+LAVD+++KQTIEALQ ELQKTKE++QA++ELK QSG
Sbjct: 405  LLESKLGHGDYSASNTKVLRMVNTLAVDNEAKQTIEALQTELQKTKEKVQALEELKSQSG 464

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            D    VDSYIS KI +LKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQ
Sbjct: 465  DTGKLVDSYISEKITKLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQ 524

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            R +GIPVT FTLQSIYAQ+DDEKL+FEYESGNTNIL NDYT Q +IS QV+IFVRK+NSI
Sbjct: 525  RPNGIPVTHFTLQSIYAQNDDEKLEFEYESGNTNILANDYTAQPEISHQVDIFVRKLNSI 584

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFN+RTLS
Sbjct: 585  PAFTANLTVESFNRRTLS 602


>gb|KJB67108.1| hypothetical protein B456_010G175200 [Gossypium raimondii]
          Length = 632

 Score =  535 bits (1378), Expect = 0.0
 Identities = 286/438 (65%), Positives = 347/438 (79%), Gaps = 3/438 (0%)
 Frame = -3

Query: 1426 QSEVREARKWKSSHEDIXXXXXXXXXXXXXXXXXXXEISKLSEAHVNAKKLEDELSSWES 1247
            +SEVREARK KSS+EDI                   E+SKL E  ++   LE+ELS W+ 
Sbjct: 195  ESEVREARKLKSSYEDIELLKAKLLEEKGRRERAESELSKLQEQQISLSNLENELSLWKL 254

Query: 1246 MMKEIPGVTSAAEIPLKFEALRKEVLEVMMKMGETSARLKQVEVALDAAENARQNAETES 1067
            +MK+IPGV+   +IP+KF  L+KEV+E  MK+GET+ARLKQ+EV LDAA  A+QNAE E+
Sbjct: 255  IMKDIPGVSCPEDIPVKFATLQKEVIESTMKIGETNARLKQIEVGLDAAHLAKQNAEAEA 314

Query: 1066 AXXXXXXXXXXXXXXXXXXXLSSVTEERDHLKTSLDQLKLQKNVEAGAAMSDVTV-KELE 890
            A                   LS VTEER+ L  ++++LK  KN EAG  ++  TV +ELE
Sbjct: 315  ALDKEKAEILKSEVKRIELMLSMVTEERNKLSNAINELKRHKNEEAGDEVASGTVVQELE 374

Query: 889  SSLAMKEDYIKKLESSIGEQKDLNARQHNEIKLLNERLNSESRRIKSLEREDDRLRSEIA 710
            SSLA KE  IK+LE S+ EQK++N RQHNEIK+L++RLN+E+RRIKSL+RE DRLRSEI+
Sbjct: 375  SSLAKKESCIKELERSLHEQKEINDRQHNEIKMLHDRLNNEARRIKSLDRESDRLRSEIS 434

Query: 709  ILESKLGHGDFSSASTKVLRMVNSLAVDSDSKQTIEALQNELQKTKEQLQAIDELKRQSG 530
            +LESKLGHGD+S+++TKVLRMVN+LAVD+++KQTIEALQ ELQKTKE++QA++ELK QSG
Sbjct: 435  LLESKLGHGDYSASNTKVLRMVNTLAVDNEAKQTIEALQTELQKTKEKVQALEELKSQSG 494

Query: 529  DAP--VDSYISGKIVQLKEQIATLEKREERYKTVFAERISVFRRACCELFGYKIVMDDHQ 356
            D    VDSYIS KI +LKEQIATLEKREERYKTVFA+RISVFRRACCELFGYKIVMD+HQ
Sbjct: 495  DTGKLVDSYISEKITKLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQ 554

Query: 355  RSDGIPVTRFTLQSIYAQSDDEKLQFEYESGNTNILGNDYTKQRDISQQVEIFVRKMNSI 176
            R +GIPVT FTLQSIYAQ+DDEKL+FEYESGNTNIL NDYT Q +IS QV+IFVRK+NSI
Sbjct: 555  RPNGIPVTHFTLQSIYAQNDDEKLEFEYESGNTNILANDYTAQPEISHQVDIFVRKLNSI 614

Query: 175  PAFTANLTVESFNKRTLS 122
            PAFTANLTVESFN+RTLS
Sbjct: 615  PAFTANLTVESFNRRTLS 632