BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00014318
         (2499 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_023728568.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   926   0.0  
gb|PNT51821.1| hypothetical protein POPTR_002G260700v3 [Populus ...   861   0.0  
ref|XP_010263670.1| PREDICTED: S-adenosyl-L-methionine-dependent...   860   0.0  
ref|XP_015884714.1| PREDICTED: S-adenosyl-L-methionine-dependent...   858   0.0  
ref|XP_022144338.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   857   0.0  
gb|PNT51820.1| hypothetical protein POPTR_002G260700v3 [Populus ...   856   0.0  
ref|XP_011024424.1| PREDICTED: S-adenosyl-L-methionine-dependent...   855   0.0  
ref|XP_012071843.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   853   0.0  
ref|XP_009364784.1| PREDICTED: S-adenosyl-L-methionine-dependent...   851   0.0  
ref|XP_023546392.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   849   0.0  
ref|XP_009336348.1| PREDICTED: S-adenosyl-L-methionine-dependent...   849   0.0  
ref|XP_021601254.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   849   0.0  
ref|XP_008377398.1| PREDICTED: S-adenosyl-L-methionine-dependent...   848   0.0  
ref|XP_008444711.1| PREDICTED: S-adenosyl-L-methionine-dependent...   848   0.0  
ref|XP_024021382.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   848   0.0  
ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent...   847   0.0  
gb|EOY14757.1| Flavodoxin family protein / radical SAM domain-co...   847   0.0  
ref|XP_022997485.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   847   0.0  
ref|XP_003550379.1| PREDICTED: S-adenosyl-L-methionine-dependent...   847   0.0  
ref|XP_022962153.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   846   0.0  

>ref|XP_023728568.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Lactuca sativa]
 gb|PLY77900.1| hypothetical protein LSAT_1X17721 [Lactuca sativa]
          Length = 659

 Score =  926 bits (2394), Expect = 0.0
 Identities = 453/599 (75%), Positives = 499/599 (83%), Gaps = 9/599 (1%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TS+ LA+ L  +LT+ NL F+L++P  YEPEDL+ E+LVLIIASTW+DG  P
Sbjct: 62   LFFISQTGTSRTLAQRLFNLLTSNNLPFELVDPNVYEPEDLHQENLVLIIASTWEDGNPP 121

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
            SNGVFLSNWL+ES  DFRVGS             GSR+YG+  FNAVG+  S+ M++LGA
Sbjct: 122  SNGVFLSNWLSESAEDFRVGSLLLSKCKFAVFGVGSRAYGDN-FNAVGRGLSTKMRSLGA 180

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVL---------ENGKNXXXXXXXXXX 1735
             E+LP+C            FG W ++V++VLK+G L         ENG            
Sbjct: 181  SEILPLCEGDVDEGELDKAFGIWSKEVIKVLKAGELRGNGNDLIEENGYEMIDGSDYEDD 240

Query: 1734 XXXXXXXXXXXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLT 1555
                            IVDLEDIAGKAP+KKD+  GA+  NGKKNG KEMVTPVIRASL 
Sbjct: 241  DDDYEDEDEENGMESDIVDLEDIAGKAPSKKDM--GAITSNGKKNGQKEMVTPVIRASLK 298

Query: 1554 KQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWR 1375
            KQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWR
Sbjct: 299  KQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWR 358

Query: 1374 HHTNPVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLV 1195
            HHTNPVGKSWQW+MDDPLMIVNSA+++HKKMIKQMKGVPGVTAERLSEGL PRHCALSLV
Sbjct: 359  HHTNPVGKSWQWKMDDPLMIVNSALEEHKKMIKQMKGVPGVTAERLSEGLLPRHCALSLV 418

Query: 1194 GEPIMYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAID 1015
            GEPIMYPEIN+LVDELH+RRISTFLVTNAQFPEKI+SLKPITQLYVSVDAA KDSMKAID
Sbjct: 419  GEPIMYPEINSLVDELHKRRISTFLVTNAQFPEKIVSLKPITQLYVSVDAATKDSMKAID 478

Query: 1014 RPLFGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGV 835
            RPLFGDFWERF DSLKALKDKQQRTVYRLTLVKGWNTE+VDAYSSLF IGNPDFVEIKGV
Sbjct: 479  RPLFGDFWERFGDSLKALKDKQQRTVYRLTLVKGWNTEDVDAYSSLFGIGNPDFVEIKGV 538

Query: 834  TYCGTSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVD 655
            TYCGTSATSKLTMENVPWHADVK FSEALA+KSNG+YE+ACEHVHSCCVLLAKVDKFK++
Sbjct: 539  TYCGTSATSKLTMENVPWHADVKAFSEALAEKSNGDYEIACEHVHSCCVLLAKVDKFKIN 598

Query: 654  GQWHTWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            GQWHTWIDY+KFHDLVASG+PF SNDYMA TPSW VYGANEGGFDPDQ RYKKERNHK+
Sbjct: 599  GQWHTWIDYDKFHDLVASGEPFGSNDYMAPTPSWTVYGANEGGFDPDQLRYKKERNHKS 657


>gb|PNT51821.1| hypothetical protein POPTR_002G260700v3 [Populus trichocarpa]
          Length = 636

 Score =  861 bits (2224), Expect = 0.0
 Identities = 428/598 (71%), Positives = 477/598 (79%), Gaps = 8/598 (1%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  LH +L + NL FDLI+P+ YEPEDL  E LV+IIASTW+DG  P
Sbjct: 44   LFFISQTGTSKTLAHRLHNLLASNNLHFDLIDPQNYEPEDLFKEKLVIIIASTWEDGKPP 103

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  F +NWL ES  DFRVGS             GSR+YGE  FNAV K FS  ++ LGA
Sbjct: 104  QNAKFFANWLAESADDFRVGSLMLSECKFAVFGVGSRAYGET-FNAVAKDFSRRLRDLGA 162

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             EV+ +            VF +W   + RVLK   +ENG+                    
Sbjct: 163  KEVVGVGEGDVDGGELDGVFEEWSGRLARVLKGESVENGE---VCGNGVVVIDESDDDED 219

Query: 1707 XXXXXXGIVDLEDIAGKAPTKKDLEKGAVAR--------NGKKNGVKEMVTPVIRASLTK 1552
                   I+DLEDIAGK P+++ L   AVA+        NGK NG KEMVTPVIRASL K
Sbjct: 220  DGGVESDIIDLEDIAGKGPSRRSL---AVAQSNGKLNGENGKLNGQKEMVTPVIRASLEK 276

Query: 1551 QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRH 1372
            QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRH
Sbjct: 277  QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRH 336

Query: 1371 HTNPVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVG 1192
            HTNPVGKSWQW+MDDPL IVNSAID H KMIKQMKGVPGVT ERL+EGLSPRHCALSLVG
Sbjct: 337  HTNPVGKSWQWKMDDPLEIVNSAIDLHTKMIKQMKGVPGVTLERLNEGLSPRHCALSLVG 396

Query: 1191 EPIMYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDR 1012
            EPIMYPEINTLVDELH+R+ISTFLVTNAQFPEKI+ LKP+TQLYVSVDAA K+S+KAIDR
Sbjct: 397  EPIMYPEINTLVDELHRRQISTFLVTNAQFPEKILMLKPVTQLYVSVDAATKESLKAIDR 456

Query: 1011 PLFGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVT 832
            PLFGDFWERF+DSLK+LK+KQQRTVYRLTLVKGWNTE++DAY +LFS+G PDF+EIKGVT
Sbjct: 457  PLFGDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDLDAYFNLFSVGQPDFIEIKGVT 516

Query: 831  YCGTSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDG 652
            YCG+SATSKLTMENVPWH+DVK FSEA+A KS GEYEVACEHVHSCCVLLAK +KFKVDG
Sbjct: 517  YCGSSATSKLTMENVPWHSDVKAFSEAMALKSKGEYEVACEHVHSCCVLLAKTEKFKVDG 576

Query: 651  QWHTWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            QW TWIDY+KFHDLVASGKPFDS DYMA TPSWAVYGA+EGGFDPDQ+RY+KER+HKT
Sbjct: 577  QWFTWIDYDKFHDLVASGKPFDSKDYMAATPSWAVYGADEGGFDPDQSRYRKERHHKT 634


>ref|XP_010263670.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Nelumbo nucifera]
          Length = 652

 Score =  860 bits (2223), Expect = 0.0
 Identities = 424/593 (71%), Positives = 474/593 (79%), Gaps = 4/593 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F++S+T TSK LA  L  IL + ++ FDL++P+ YEPEDL+ E LVLI+ASTW+DG  P
Sbjct: 60   LFFVSQTGTSKTLAHRLFDILNSNSIPFDLVDPKDYEPEDLSKETLVLIVASTWEDGKPP 119

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  FL+NWL ESV DFRVGS             GS+SYGE  FNAV K FS  M+ALGA
Sbjct: 120  PNSNFLANWLAESVDDFRVGSLLFSDCKFAVFGVGSQSYGET-FNAVAKDFSKRMRALGA 178

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             EVLP+C           VFG W   +VRVLK G+LENGK                    
Sbjct: 179  SEVLPVCEGDVDAGDLDAVFGAWSWKLVRVLKGGLLENGKPPGTALFTESEVESVDDSED 238

Query: 1707 XXXXXXG----IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYK 1540
                  G    IVDLEDIAGKAP +K     +   NG+ NG KEMVTPVIR SL KQGYK
Sbjct: 239  DYIEENGPESVIVDLEDIAGKAPPRKS--STSAVTNGEVNGKKEMVTPVIRMSLEKQGYK 296

Query: 1539 IIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 1360
            IIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP
Sbjct: 297  IIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNP 356

Query: 1359 VGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIM 1180
            VGKSWQW+MDDPL I+N AIDQH KMIKQMKGVPGVT ERLSEGL P+HCALSLVGEPIM
Sbjct: 357  VGKSWQWKMDDPLEIINGAIDQHTKMIKQMKGVPGVTLERLSEGLLPKHCALSLVGEPIM 416

Query: 1179 YPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFG 1000
            YPEIN LVDELH+RRISTFLVTNAQFPE+I  LKP+TQLYVSVDAA KDS+KAIDRPLFG
Sbjct: 417  YPEINILVDELHRRRISTFLVTNAQFPERIRMLKPVTQLYVSVDAATKDSLKAIDRPLFG 476

Query: 999  DFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGT 820
            DFWERF+DSLKAL++K+QRTV+RLTLVKGWN E++DAYS L +IG PDF+EIKGVTYCG+
Sbjct: 477  DFWERFIDSLKALQEKEQRTVFRLTLVKGWNIEDIDAYSKLINIGKPDFIEIKGVTYCGS 536

Query: 819  SATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHT 640
            SATSKLTMENVPWH DVK FSEALA KSNGEYEVACEHVHSCCVLLAKV+KFK++GQW T
Sbjct: 537  SATSKLTMENVPWHTDVKAFSEALASKSNGEYEVACEHVHSCCVLLAKVEKFKINGQWFT 596

Query: 639  WIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHK 481
            WIDYEKFHDLVAS KPF SNDY+A TPSWA+YGA +GGFDPDQ+R++KER+H+
Sbjct: 597  WIDYEKFHDLVASEKPFSSNDYVAATPSWALYGAEDGGFDPDQSRHRKERHHR 649


>ref|XP_015884714.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Ziziphus jujuba]
          Length = 647

 Score =  858 bits (2216), Expect = 0.0
 Identities = 422/591 (71%), Positives = 471/591 (79%), Gaps = 1/591 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  L  +LT+ NL FDL++P  YEPEDL  E+LVLI+ASTW+DG +P
Sbjct: 56   LFFISQTGTSKALAVRLFDLLTSNNLAFDLVDPNHYEPEDLPKENLVLIVASTWEDGKAP 115

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
                F +NWL+ES  DFRVGS             GSR+YG   FNAV K  S  M+ALGA
Sbjct: 116  PTAKFFANWLSESSDDFRVGSLLLSQCKFAVFGVGSRAYGAT-FNAVAKDVSKQMRALGA 174

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGK-NXXXXXXXXXXXXXXXXXX 1711
             E+LP+            VF  W   VV   K GV+ENG  +                  
Sbjct: 175  KEILPVWEGDVDGGDIDEVFDAWSGKVVGFCKGGVVENGGVSTNGVDCESDACSGDETDE 234

Query: 1710 XXXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIG 1531
                    IVDLEDIAGK P+++         NG  NG K+MVTPVIRASL KQGYKIIG
Sbjct: 235  EEYSMESEIVDLEDIAGKGPSRRSATVTKAETNGTLNGKKDMVTPVIRASLEKQGYKIIG 294

Query: 1530 SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 1351
            SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 295  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 354

Query: 1350 SWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPE 1171
            SWQW+MDDPL IVNSAI+QH KMIKQMKGVPGVT ERL EGLSPRHCALSLVGEPIMYPE
Sbjct: 355  SWQWKMDDPLEIVNSAIEQHTKMIKQMKGVPGVTVERLREGLSPRHCALSLVGEPIMYPE 414

Query: 1170 INTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFW 991
            INTLVDELH+RR+STFLVTNAQFPEKI  LKPITQLYVSVDAA K+S+KAIDRPLFGDFW
Sbjct: 415  INTLVDELHRRRVSTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLFGDFW 474

Query: 990  ERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSAT 811
            ER +DSLKALK+KQQRTVYRLTLVKGWNTE+++AYS+LFSIG PDF+EIKGVTYCG+SAT
Sbjct: 475  ERLIDSLKALKEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGKPDFIEIKGVTYCGSSAT 534

Query: 810  SKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWID 631
            SKLTMENVPWH+DV++FSEALA +S GEYEVACEHVHSCCVLLAK DKFK++GQW TWID
Sbjct: 535  SKLTMENVPWHSDVRDFSEALALRSLGEYEVACEHVHSCCVLLAKTDKFKINGQWFTWID 594

Query: 630  YEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            YEKFHDLVASG+PFDS DYMA+TPSWAVYGA EGGFDPDQ+RYKKER+H++
Sbjct: 595  YEKFHDLVASGRPFDSKDYMAITPSWAVYGAEEGGFDPDQSRYKKERHHRS 645


>ref|XP_022144338.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Momordica charantia]
          Length = 640

 Score =  857 bits (2214), Expect = 0.0
 Identities = 422/592 (71%), Positives = 472/592 (79%), Gaps = 1/592 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+ISET TSK LA  L  +L +  L FDL++PE YEPEDL  E LVL++ASTW+DG  P
Sbjct: 48   LFFISETGTSKALAHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLLVASTWEDGGPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             +  F +NWL+ES  DFRVGS             GSR+YG+  FNAV + FS  M+ALGA
Sbjct: 108  PHAKFFANWLSESAEDFRVGSLLLSECKFGVFGVGSRAYGDT-FNAVARGFSKQMRALGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+LP+             F  W + V++VLK   + +G +                   
Sbjct: 167  KEILPVGEGDVDGGDIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESVEDDEE 226

Query: 1707 XXXXXXG-IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIG 1531
                    IVDLEDIAGKAP++K      V  NGK NG KEMVTPVIRASL KQGYKIIG
Sbjct: 227  DADLGAEDIVDLEDIAGKAPSRKSTN--VVETNGKLNGKKEMVTPVIRASLEKQGYKIIG 284

Query: 1530 SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 1351
            +HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 285  THSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 344

Query: 1350 SWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPE 1171
            SWQW+MDDPL IVNSAID H KMIKQMKGVPGVT E+L+EGLSPRHCALSLVGEPIMYPE
Sbjct: 345  SWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPE 404

Query: 1170 INTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFW 991
            INTLVDELH+RRISTFLVTNAQFP+KI SLKP+TQLYVSVDAA K+S+KAIDRPLFGDFW
Sbjct: 405  INTLVDELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFW 464

Query: 990  ERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSAT 811
            ERF+DSLKAL DKQQRTVYRLTLVKGWN E++DAYS+LFSIG PDFVEIKGVTYCG+S T
Sbjct: 465  ERFIDSLKALNDKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNT 524

Query: 810  SKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWID 631
            SKLTMENVPWH+DVK FSEALA KS G+YEVACEHVHSCCVLLAK DKF+V+GQW TWID
Sbjct: 525  SKLTMENVPWHSDVKSFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWID 584

Query: 630  YEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKTK 475
            YEKFHDLVASGKPFDS DYMA TPSWAVYG++EGGFDPDQ+RY+KER+H+ K
Sbjct: 585  YEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPK 636


>gb|PNT51820.1| hypothetical protein POPTR_002G260700v3 [Populus trichocarpa]
          Length = 637

 Score =  856 bits (2212), Expect = 0.0
 Identities = 428/599 (71%), Positives = 477/599 (79%), Gaps = 9/599 (1%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  LH +L + NL FDLI+P+ YEPEDL  E LV+IIASTW+DG  P
Sbjct: 44   LFFISQTGTSKTLAHRLHNLLASNNLHFDLIDPQNYEPEDLFKEKLVIIIASTWEDGKPP 103

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  F +NWL ES  DFRVGS             GSR+YGE  FNAV K FS  ++ LGA
Sbjct: 104  QNAKFFANWLAESADDFRVGSLMLSECKFAVFGVGSRAYGET-FNAVAKDFSRRLRDLGA 162

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             EV+ +            VF +W   + RVLK   +ENG+                    
Sbjct: 163  KEVVGVGEGDVDGGELDGVFEEWSGRLARVLKGESVENGE---VCGNGVVVIDESDDDED 219

Query: 1707 XXXXXXGIVDLEDIAGKAPTKKDLEKGAVAR--------NGKKNGVKEMVTPVIRASLTK 1552
                   I+DLEDIAGK P+++ L   AVA+        NGK NG KEMVTPVIRASL K
Sbjct: 220  DGGVESDIIDLEDIAGKGPSRRSL---AVAQSNGKLNGENGKLNGQKEMVTPVIRASLEK 276

Query: 1551 QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH-RCMEATPSLACANKCVFCWR 1375
            QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH RCMEATPSLACANKCVFCWR
Sbjct: 277  QGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHSRCMEATPSLACANKCVFCWR 336

Query: 1374 HHTNPVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLV 1195
            HHTNPVGKSWQW+MDDPL IVNSAID H KMIKQMKGVPGVT ERL+EGLSPRHCALSLV
Sbjct: 337  HHTNPVGKSWQWKMDDPLEIVNSAIDLHTKMIKQMKGVPGVTLERLNEGLSPRHCALSLV 396

Query: 1194 GEPIMYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAID 1015
            GEPIMYPEINTLVDELH+R+ISTFLVTNAQFPEKI+ LKP+TQLYVSVDAA K+S+KAID
Sbjct: 397  GEPIMYPEINTLVDELHRRQISTFLVTNAQFPEKILMLKPVTQLYVSVDAATKESLKAID 456

Query: 1014 RPLFGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGV 835
            RPLFGDFWERF+DSLK+LK+KQQRTVYRLTLVKGWNTE++DAY +LFS+G PDF+EIKGV
Sbjct: 457  RPLFGDFWERFIDSLKSLKEKQQRTVYRLTLVKGWNTEDLDAYFNLFSVGQPDFIEIKGV 516

Query: 834  TYCGTSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVD 655
            TYCG+SATSKLTMENVPWH+DVK FSEA+A KS GEYEVACEHVHSCCVLLAK +KFKVD
Sbjct: 517  TYCGSSATSKLTMENVPWHSDVKAFSEAMALKSKGEYEVACEHVHSCCVLLAKTEKFKVD 576

Query: 654  GQWHTWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            GQW TWIDY+KFHDLVASGKPFDS DYMA TPSWAVYGA+EGGFDPDQ+RY+KER+HKT
Sbjct: 577  GQWFTWIDYDKFHDLVASGKPFDSKDYMAATPSWAVYGADEGGFDPDQSRYRKERHHKT 635


>ref|XP_011024424.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Populus euphratica]
 ref|XP_011024425.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Populus euphratica]
 ref|XP_011024426.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Populus euphratica]
 ref|XP_011024427.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Populus euphratica]
          Length = 638

 Score =  855 bits (2208), Expect = 0.0
 Identities = 425/595 (71%), Positives = 471/595 (79%), Gaps = 5/595 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  LH +L + NL FDLI+P+ YEPEDL  E LV+IIASTW+DG  P
Sbjct: 46   LFFISQTGTSKTLAHRLHNLLASNNLHFDLIDPQNYEPEDLFKEKLVIIIASTWEDGKPP 105

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  F +NWL ES  DFRVGS             GSR++GE  FNAV K FS  ++ LGA
Sbjct: 106  QNAKFFANWLAESADDFRVGSLMLSECKFAVFGVGSRAFGET-FNAVAKDFSRRLRDLGA 164

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             EV+ +            VF +W   + RVLK   +ENG+                    
Sbjct: 165  KEVVGVGEGDVDGGELDGVFEEWSGRLARVLKGESVENGE---VCGNGLVVIDESDDDED 221

Query: 1707 XXXXXXGIVDLEDIAGKAPTKK----DLEKGAV-ARNGKKNGVKEMVTPVIRASLTKQGY 1543
                   I+DLEDIAGK P+++    D   G +   NGK NG KEMVTPVIRASL KQGY
Sbjct: 222  DGGVESDIIDLEDIAGKGPSRRSIVVDQSNGKLNGENGKLNGQKEMVTPVIRASLEKQGY 281

Query: 1542 KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1363
            KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTN
Sbjct: 282  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTN 341

Query: 1362 PVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPI 1183
            PVGKSWQW+MDDPL IVNSAID H KMIKQMKGVPGVT ERL+EGLSPRHCALSLVGEPI
Sbjct: 342  PVGKSWQWKMDDPLEIVNSAIDLHTKMIKQMKGVPGVTLERLNEGLSPRHCALSLVGEPI 401

Query: 1182 MYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLF 1003
            MYPEINTLVDELH+R+ISTFLVTNAQFPEKI  LKP+TQLYVSVDAA K+S+KAIDRPLF
Sbjct: 402  MYPEINTLVDELHRRQISTFLVTNAQFPEKIQMLKPVTQLYVSVDAATKESLKAIDRPLF 461

Query: 1002 GDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCG 823
            GDFWERF+DSLK+L +KQQRTVYRLTLVKGWNTE+VDAY +LFS+G PDF+EIKGVTYCG
Sbjct: 462  GDFWERFIDSLKSLNEKQQRTVYRLTLVKGWNTEDVDAYFNLFSVGQPDFIEIKGVTYCG 521

Query: 822  TSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWH 643
            +SATSKLTMENVPWH+DVK FSEALA KS GEYEVACEHVHSCCVLLAK +KFKVDGQW 
Sbjct: 522  SSATSKLTMENVPWHSDVKAFSEALAFKSKGEYEVACEHVHSCCVLLAKTEKFKVDGQWF 581

Query: 642  TWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            TWIDY+KFHDLVASGKPFDS DYMA TPSWAVYGA EGGFDPDQ+RY+KER+HKT
Sbjct: 582  TWIDYDKFHDLVASGKPFDSKDYMAATPSWAVYGAEEGGFDPDQSRYRKERHHKT 636


>ref|XP_012071843.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Jatropha curcas]
 ref|XP_012071844.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Jatropha curcas]
 ref|XP_020534805.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Jatropha curcas]
 gb|KDP38500.1| hypothetical protein JCGZ_04425 [Jatropha curcas]
          Length = 650

 Score =  853 bits (2204), Expect = 0.0
 Identities = 421/596 (70%), Positives = 469/596 (78%), Gaps = 6/596 (1%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSKNLA+ LH +    +L FDLI+ + YEPEDL+ E LV+I+ASTW+DG  P
Sbjct: 56   LFFISQTGTSKNLAQRLHNLFKLNDLSFDLIDAKDYEPEDLSKETLVIIVASTWEDGKPP 115

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
            SN  F SNWL ES  DFRVGS             GSR+YG+  FNAV K FS  ++ LGA
Sbjct: 116  SNANFFSNWLAESADDFRVGSLLLTNCKFAVFGVGSRAYGDT-FNAVAKDFSRRLRELGA 174

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+L +             F +W E VV +LK G +ENG+                    
Sbjct: 175  NEILTVGEGDVDGGELDGFFEEWSEKVVGILKGGRVENGEVFINGCIGSESDNENIVDEF 234

Query: 1707 XXXXXXG------IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQG 1546
                         IVDLEDIAGK PT+K      V  NGK NG KEMVTPVIRA+L KQG
Sbjct: 235  GSDDDENVGVESGIVDLEDIAGKGPTRKF--NAVVETNGKLNGQKEMVTPVIRANLEKQG 292

Query: 1545 YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT 1366
            YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT
Sbjct: 293  YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT 352

Query: 1365 NPVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEP 1186
            NPVGK+WQW+MDDPL IVNSAIDQH KMIKQMKGVPGVT ERL+EGL PRHCALSLVGEP
Sbjct: 353  NPVGKNWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLHPRHCALSLVGEP 412

Query: 1185 IMYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPL 1006
            IMYPEINTLVDELH+RRISTFLVTNAQFPEKI  LKP+TQLYVSVDAA K+S+K+IDRPL
Sbjct: 413  IMYPEINTLVDELHRRRISTFLVTNAQFPEKITMLKPVTQLYVSVDAATKESLKSIDRPL 472

Query: 1005 FGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYC 826
            FGDFWERF+DSLKALK+KQQRTVYRLTLVKGWNTE++D Y  LFSIG PDF+EIKGVTYC
Sbjct: 473  FGDFWERFIDSLKALKEKQQRTVYRLTLVKGWNTEDIDDYFKLFSIGQPDFIEIKGVTYC 532

Query: 825  GTSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQW 646
            G+SATSKLTMENVPWHADVK FSEALA KS GEYEVACEH HSCCVLLA+ +KFK++GQW
Sbjct: 533  GSSATSKLTMENVPWHADVKAFSEALALKSKGEYEVACEHAHSCCVLLARTEKFKINGQW 592

Query: 645  HTWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
             TWIDYEKFHDLVASGKPF+S DY+A TP WAVYGA EGGFDPDQ+RY+KER+HK+
Sbjct: 593  FTWIDYEKFHDLVASGKPFNSKDYVAATPFWAVYGAQEGGFDPDQSRYRKERHHKS 648


>ref|XP_009364784.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Pyrus x bretschneideri]
          Length = 644

 Score =  851 bits (2199), Expect = 0.0
 Identities = 426/595 (71%), Positives = 462/595 (77%), Gaps = 5/595 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F++S+T TS+ LA+ L   L  K + FDL++P  YEPEDL  E L LI+ASTW DG  P
Sbjct: 50   LFFVSQTGTSEALARRLLDFLKLKGVAFDLVDPNNYEPEDLPKETLFLIVASTWDDGKPP 109

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  F SNWL ES  DFRVGS             GSR+YG   FNAVGKA +  M+ALGA
Sbjct: 110  PNAKFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGAT-FNAVGKALARRMRALGA 168

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+ PI            VF  W   VVR LK G  ENG                     
Sbjct: 169  SEMFPIWEGDVDGGDLDEVFDAWSGKVVRFLKGGAAENGGVLGNGVSDECEAESFDVSDE 228

Query: 1707 XXXXXXG-----IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGY 1543
                        +VDLEDIAGK P++++    A   NG  NG KEMVTPVIRASLTKQGY
Sbjct: 229  EDEEEEDGGESGVVDLEDIAGKGPSRRN-PVAATKTNGTVNGKKEMVTPVIRASLTKQGY 287

Query: 1542 KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1363
            KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTN
Sbjct: 288  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTN 347

Query: 1362 PVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPI 1183
            PVGKSWQW+MDDPL IVNSAIDQH KMIKQMKGVPGVT ERL+EGLSPRHCALSLVGEPI
Sbjct: 348  PVGKSWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPI 407

Query: 1182 MYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLF 1003
            MYPEIN L+DELHQRRISTFLVTNAQFPEKI  L PITQLYVSVDAA K+S+KAIDRPLF
Sbjct: 408  MYPEINALIDELHQRRISTFLVTNAQFPEKIRMLNPITQLYVSVDAATKESLKAIDRPLF 467

Query: 1002 GDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCG 823
            GDFWERF+DSL +LKDKQQRTVYRLTLVKGWNTE++DAY ++FS+G+PDFVEIKGVTYCG
Sbjct: 468  GDFWERFIDSLTSLKDKQQRTVYRLTLVKGWNTEDIDAYYNIFSLGSPDFVEIKGVTYCG 527

Query: 822  TSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWH 643
            TSATSKLTMENVPWHADVK FSEALA KSNGEYEVACEHVHSCCVLLAK  KFK+DGQW 
Sbjct: 528  TSATSKLTMENVPWHADVKAFSEALALKSNGEYEVACEHVHSCCVLLAKTSKFKIDGQWF 587

Query: 642  TWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            TWIDYEKFHDLVASGK FDS DYMA TPSWAVYGA EGGFDP Q+RYKKER+H+T
Sbjct: 588  TWIDYEKFHDLVASGKHFDSKDYMAPTPSWAVYGAEEGGFDPYQSRYKKERHHRT 642


>ref|XP_023546392.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Cucurbita pepo subsp. pepo]
          Length = 639

 Score =  849 bits (2194), Expect = 0.0
 Identities = 421/592 (71%), Positives = 467/592 (78%), Gaps = 1/592 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  L  +L +  L FD+++P+ +EPEDL  E LVL +ASTW+DG  P
Sbjct: 48   LFFISQTGTSKALAHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             +  FL++WL+ES  DFRVGS             GSR+YGE  FNAV + FS  MKALGA
Sbjct: 108  PHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGET-FNAVARDFSKRMKALGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+L +             F  W   +++VLK   +  G +                   
Sbjct: 167  KEILAVGEGDVDGGDIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDE 226

Query: 1707 XXXXXXG-IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIG 1531
                    IVDLEDIAGK P++K      V  NGK NG K MVTPVIRASL KQGYKIIG
Sbjct: 227  DADSGEEDIVDLEDIAGKGPSRKSTN--VVETNGKLNGKKVMVTPVIRASLEKQGYKIIG 284

Query: 1530 SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 1351
            SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 285  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 344

Query: 1350 SWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPE 1171
            SWQW+MDDPL IVNSAID HKKMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPE
Sbjct: 345  SWQWEMDDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPE 404

Query: 1170 INTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFW 991
            INTLVDELHQRRISTFLVTNAQFP+KI SLKP+TQLYVSVDAA K+S+KAIDRPLFGDFW
Sbjct: 405  INTLVDELHQRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFW 464

Query: 990  ERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSAT 811
            ERF+DSLKAL DKQQRTVYRLTLVKGWNTE++DAYS+LFS+G PDFVEIKGVTYCG+S T
Sbjct: 465  ERFIDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNT 524

Query: 810  SKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWID 631
            SKLTMENVPWH+DVK FSEALA KS GEYEVACEHVHSCCVLLAK DKF+VDGQW TWID
Sbjct: 525  SKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWID 584

Query: 630  YEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKTK 475
            YEKFHDLVASGKPFDS DYMA TPSWAVYGA+EGGFDP Q+RY+KER+H+ K
Sbjct: 585  YEKFHDLVASGKPFDSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPK 636


>ref|XP_009336348.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Pyrus x bretschneideri]
          Length = 644

 Score =  849 bits (2194), Expect = 0.0
 Identities = 425/595 (71%), Positives = 462/595 (77%), Gaps = 5/595 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F++S+T TS+ LA+ L   L  K + FDL++P  YEPEDL  E LVLI+ASTW DG  P
Sbjct: 50   LFFVSQTGTSEALARRLLDFLKLKGVAFDLVDPNNYEPEDLPKETLVLIVASTWDDGKPP 109

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  F SNWL ES  DFRVGS             GSR+YG   FNAVGK  +  M+ALGA
Sbjct: 110  PNAKFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGAT-FNAVGKDLARRMRALGA 168

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+ PI            VF  W   VVR LK G  ENG                     
Sbjct: 169  SEMFPIWEGDVDGGDLDEVFDAWSGKVVRFLKGGAAENGGVLGNGVSDECEAESFDVSDK 228

Query: 1707 XXXXXXG-----IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGY 1543
                        +VDLEDIAGK P++++    A   NG  NG KEMVTPVIRASLTKQGY
Sbjct: 229  EDEEEEDGGESGVVDLEDIAGKGPSRRN-PVAATKTNGTVNGKKEMVTPVIRASLTKQGY 287

Query: 1542 KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTN 1363
            KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTN
Sbjct: 288  KIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTN 347

Query: 1362 PVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPI 1183
            PVGKSWQW+MDDPL IVNSAIDQH KMIKQMKGVPGVT ERL+EGLSPRHCALSLVGEPI
Sbjct: 348  PVGKSWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPI 407

Query: 1182 MYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLF 1003
            MYPEIN L+DELH+RRISTFLVTNAQFPEKI  L PITQLYVSVDAA K+S+KAIDRPLF
Sbjct: 408  MYPEINALIDELHRRRISTFLVTNAQFPEKIKMLNPITQLYVSVDAATKESLKAIDRPLF 467

Query: 1002 GDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCG 823
            GDFWERF+DSL +LKDKQQRTVYRLTLVKGWNTE++DAY ++FS+G+PDFVEIKGVTYCG
Sbjct: 468  GDFWERFIDSLTSLKDKQQRTVYRLTLVKGWNTEDIDAYYNIFSLGSPDFVEIKGVTYCG 527

Query: 822  TSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWH 643
            TSATSKLTMENVPWHADVK FSEALA KSNGEYEVACEHVHSCCVLLAK  KFK+DGQW 
Sbjct: 528  TSATSKLTMENVPWHADVKAFSEALALKSNGEYEVACEHVHSCCVLLAKTSKFKIDGQWF 587

Query: 642  TWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            TWIDYEKFHDLVASGK FDS DYMA TPSWAVYGA EGGFDP Q+RYKKER+H+T
Sbjct: 588  TWIDYEKFHDLVASGKHFDSKDYMAPTPSWAVYGAEEGGFDPYQSRYKKERHHRT 642


>ref|XP_021601254.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Manihot esculenta]
 ref|XP_021601255.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Manihot esculenta]
 ref|XP_021601256.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Manihot esculenta]
 gb|OAY22798.1| hypothetical protein MANES_18G027000 [Manihot esculenta]
 gb|OAY22799.1| hypothetical protein MANES_18G027000 [Manihot esculenta]
          Length = 650

 Score =  849 bits (2194), Expect = 0.0
 Identities = 423/596 (70%), Positives = 469/596 (78%), Gaps = 6/596 (1%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+I++T TSK LA+ LH +L   +L FDLI+ + YEPEDL+ E LV+IIASTW+DG  P
Sbjct: 56   LFFITQTGTSKTLAQRLHNLLKLNDLPFDLIDIKDYEPEDLSKETLVIIIASTWEDGKPP 115

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
            SN  F  NWL ES  DFRVGS             GS +YG+  FNAV K FS  ++ LGA
Sbjct: 116  SNASFFGNWLAESADDFRVGSLLLSKCKFSVFGVGSAAYGDT-FNAVAKGFSRRLRDLGA 174

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSG------VLENGKNXXXXXXXXXXXXX 1726
             E++ +            +F +W   V+R+LK G      V  NG               
Sbjct: 175  NEIVEVGEGDVDGGELDLIFEEWSGKVLRILKGGSVQNGTVFSNGCVGGESDNENIEDGF 234

Query: 1725 XXXXXXXXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQG 1546
                        GIVDLEDIAGK P+++      V  N K NG KEMVTPVIRA+L KQG
Sbjct: 235  ESDNDGNGFAESGIVDLEDIAGKGPSRRSTAVAEV--NEKVNGQKEMVTPVIRANLEKQG 292

Query: 1545 YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT 1366
            YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT
Sbjct: 293  YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT 352

Query: 1365 NPVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEP 1186
            NPVGK+WQW+MDDPL IVNSAIDQH KMIKQMKGVPGVT ERL+EGLSPRHCALSLVGEP
Sbjct: 353  NPVGKNWQWKMDDPLEIVNSAIDQHAKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEP 412

Query: 1185 IMYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPL 1006
            IMYPEIN LVDELHQRRISTFLVTNAQFPEKI  LKP+TQLYVSVDAA KDS+KAIDRPL
Sbjct: 413  IMYPEINKLVDELHQRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKDSLKAIDRPL 472

Query: 1005 FGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYC 826
            FGDFWERF+DSLKALK+KQQRTVYRLTLVKGWNTE+VDAY +LFSIG PDF+EIKGVTYC
Sbjct: 473  FGDFWERFIDSLKALKEKQQRTVYRLTLVKGWNTEDVDAYFNLFSIGQPDFIEIKGVTYC 532

Query: 825  GTSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQW 646
            GTSATSKLTMENVPWH+DVK FSEALA KS GEYEVACEHVHSCCVLLAK +KFKV+GQW
Sbjct: 533  GTSATSKLTMENVPWHSDVKAFSEALALKSKGEYEVACEHVHSCCVLLAKTEKFKVNGQW 592

Query: 645  HTWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
             TWIDY+KFHDLVASGKPF+  DYMA TPSWAVYGA EGGFDPDQ+RY+KER+H+T
Sbjct: 593  FTWIDYDKFHDLVASGKPFNGKDYMATTPSWAVYGAQEGGFDPDQSRYRKERHHRT 648


>ref|XP_008377398.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Malus domestica]
          Length = 645

 Score =  848 bits (2191), Expect = 0.0
 Identities = 427/595 (71%), Positives = 467/595 (78%), Gaps = 6/595 (1%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F++S+T TS+ LA+ L   LT+  + FDL++P  YEPEDL  E LVLI+ASTW DG  P
Sbjct: 50   LFFVSQTGTSEALARRLLDXLTSNGVAFDLVDPXNYEPEDLPKEXLVLIVASTWDDGKPP 109

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             N  F SNWL ES  DFRVGS             GSR+YG   FNAVGK  +  M+ALGA
Sbjct: 110  PNARFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGAT-FNAVGKDLARRMRALGA 168

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSG------VLENGKNXXXXXXXXXXXXX 1726
             E+ PI            VF  W   VVR LK G      VL NG               
Sbjct: 169  SEMFPIWEXDVDGGDLDEVFDAWSGKVVRFLKGGAAENGGVLGNGVGDECEAESFDGSDE 228

Query: 1725 XXXXXXXXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQG 1546
                        G+VDLEDIAGK P++++        NG  NG KEMVTPVIRASLTKQG
Sbjct: 229  DEEDEEEDGXESGVVDLEDIAGKGPSRRN-PVAVTKTNGTVNGKKEMVTPVIRASLTKQG 287

Query: 1545 YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHT 1366
            YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHT
Sbjct: 288  YKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHT 347

Query: 1365 NPVGKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEP 1186
            NPVGKSWQW+MDDPL IVNSAIDQH KMIKQMKGVPGVT ERL+EGLSPRHCALSLVGEP
Sbjct: 348  NPVGKSWQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEP 407

Query: 1185 IMYPEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPL 1006
            IMYPEIN+L DELH+RRISTFLVTNAQFPEKI  L PITQLYVSVDAA K+S+KAIDRPL
Sbjct: 408  IMYPEINSLXDELHRRRISTFLVTNAQFPEKIKMLNPITQLYVSVDAATKESLKAIDRPL 467

Query: 1005 FGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYC 826
            FGDFWERF+DSL +LKDKQQRTVYRLTLVKGWNTE++DAY ++FS+G+PDFVEIKGVTYC
Sbjct: 468  FGDFWERFIDSLTSLKDKQQRTVYRLTLVKGWNTEDIDAYYNIFSLGSPDFVEIKGVTYC 527

Query: 825  GTSATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQW 646
            GTSATSKLTMENVPWHADVK FSEALA KSNGEYEVACEHVHSCCVLLAK  KFK++GQW
Sbjct: 528  GTSATSKLTMENVPWHADVKXFSEALALKSNGEYEVACEHVHSCCVLLAKTSKFKINGQW 587

Query: 645  HTWIDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHK 481
             TWIDYEKFHDLVASGKPFDS DYMA TPSWAVYGA+EGGFDP Q+RYKKER+H+
Sbjct: 588  FTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGADEGGFDPYQSRYKKERHHR 642


>ref|XP_008444711.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Cucumis melo]
          Length = 641

 Score =  848 bits (2190), Expect = 0.0
 Identities = 422/594 (71%), Positives = 466/594 (78%), Gaps = 3/594 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  L  +L +  L FDL++P+ YEPEDL  E LVL +ASTW+DG  P
Sbjct: 48   LFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             +  FL+NWL+ES  DFRVGS             GSR YG E +NAV + FS  MK+LGA
Sbjct: 108  PHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYG-ETYNAVARDFSKRMKSLGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGK---NXXXXXXXXXXXXXXXX 1717
             E+LPI             F  W   +++V+K     NG    +                
Sbjct: 167  KEILPIGEGDVDGGDIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYE 226

Query: 1716 XXXXXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKI 1537
                      IVDLEDIAGK P++K      V  NGK NG K MVTPVIRASL KQGYKI
Sbjct: 227  EEDDDLGEEDIVDLEDIAGKGPSRK--STNVVETNGKLNGKKVMVTPVIRASLEKQGYKI 284

Query: 1536 IGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPV 1357
            IGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPV
Sbjct: 285  IGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPV 344

Query: 1356 GKSWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMY 1177
            GKSWQW+MDDPL IVNSAID H KMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMY
Sbjct: 345  GKSWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMY 404

Query: 1176 PEINTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGD 997
            PEINTLVDELHQRRISTFLVTNAQFP+KI  LKP+TQLYVSVDAA K+S+KAIDRPLFGD
Sbjct: 405  PEINTLVDELHQRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGD 464

Query: 996  FWERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTS 817
            FWERF+DSLKAL +KQQRTVYRLTLVKGWNTE++DAYS+LFSIG PDFVEIKGVTYCG+S
Sbjct: 465  FWERFIDSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS 524

Query: 816  ATSKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTW 637
             TSKLTMENVPWH+DVK FSEALA KS GEYEVACEHVHSCCVLLAK DKF+VDGQW TW
Sbjct: 525  NTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTW 584

Query: 636  IDYEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKTK 475
            IDYEKFHDLVASGKPFDS DY+A TPSWAVYG++EGGFDPDQ+RY+KER+HK K
Sbjct: 585  IDYEKFHDLVASGKPFDSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPK 638


>ref|XP_024021382.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Morus notabilis]
          Length = 659

 Score =  848 bits (2191), Expect = 0.0
 Identities = 418/588 (71%), Positives = 464/588 (78%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            + +ISET TSK LA+ L  +L + +L FDL++ + YEPEDL  E  VLI+ASTW+DG +P
Sbjct: 64   LLFISETGTSKTLARRLLDLLNSNDLAFDLVDAKAYEPEDLPKETQVLIVASTWEDGKAP 123

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             +G F +NWL ESV DFRVGS             GSR+YG   +N V +  S  M+ LGA
Sbjct: 124  PSGRFFANWLAESVEDFRVGSLLLSQCKFAVFGVGSRAYGAT-YNVVARDLSKRMRGLGA 182

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+LP+             F  W   +V+VL   V+ENG                     
Sbjct: 183  AEMLPVWEGDVDGGDLEEAFDAWSAKMVKVLNDDVVENGGALSNGVGAESDAESDYETDE 242

Query: 1707 XXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIGS 1528
                   IVDLEDIAGK P+++      V  NG  NG K+MVTPVIRASL KQGYKIIGS
Sbjct: 243  EYSVESEIVDLEDIAGKGPSRRS--PVVVQTNGTSNGKKDMVTPVIRASLEKQGYKIIGS 300

Query: 1527 HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 1348
            HSGVK+CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 301  HSGVKVCRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 360

Query: 1347 WQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPEI 1168
            WQW+MDDPL IVNSAIDQH KMIKQMKGVPGVT ERL+EGLSPRHCALSLVGEPIMYPEI
Sbjct: 361  WQWKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPIMYPEI 420

Query: 1167 NTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFWE 988
            NTLVDELH+RRISTFLVTNAQFPEKI  LKPITQLYVSVDAA KDS+KAIDRPLFGDFWE
Sbjct: 421  NTLVDELHRRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 480

Query: 987  RFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSATS 808
            RF+DSLKAL+DKQQRTVYRLTLVKGWNTE+VDAY +LFSIG PDF+EIKGVTYCG+SATS
Sbjct: 481  RFLDSLKALRDKQQRTVYRLTLVKGWNTEDVDAYFNLFSIGKPDFIEIKGVTYCGSSATS 540

Query: 807  KLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWIDY 628
            KLTMENVPWH++VK FSEALA KS GEYEVACEHVHSCCVLLAK +KFK++GQW TWI+Y
Sbjct: 541  KLTMENVPWHSEVKAFSEALALKSQGEYEVACEHVHSCCVLLAKTEKFKINGQWFTWIEY 600

Query: 627  EKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNH 484
            EKFHDLVASGK FDS DYMA TPSWAVYGA EGGFDPDQ+RYKKER+H
Sbjct: 601  EKFHDLVASGKAFDSKDYMAATPSWAVYGAEEGGFDPDQSRYKKERHH 648


>ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Theobroma cacao]
          Length = 637

 Score =  847 bits (2188), Expect = 0.0
 Identities = 416/590 (70%), Positives = 466/590 (78%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+I++T TSK LA+ L  +L++ N+ FDL++P+ YEPEDL  E L+LIIASTW+DG  P
Sbjct: 48   LFFITQTGTSKALAQRLLNLLSSNNIPFDLVDPQNYEPEDLPKESLILIIASTWEDGNPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
                F  NWL E  +DFR G+             GSR YGE  FNAV +     ++ LGA
Sbjct: 108  QGSKFFVNWLAEISTDFRAGNLLLSNCKFAVFGVGSRVYGET-FNAVARNLGKRLRGLGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E++ +            VF +W E VVRVLK G++   +N                   
Sbjct: 167  TEMVAVGEGDVDGGALESVFKEWSEKVVRVLKGGLVVENENGIVYASDAESFESDDDDNG 226

Query: 1707 XXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIGS 1528
                   IVDLEDIAGK P++K         NGK +G +EMVTPVIRA+L KQGYKIIGS
Sbjct: 227  GGGGEEEIVDLEDIAGKGPSRKK-SVNVAETNGKMDGKREMVTPVIRANLEKQGYKIIGS 285

Query: 1527 HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 1348
            HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 286  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 345

Query: 1347 WQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPEI 1168
            WQW+MDDPL IVN+AID H KMIKQMKGVPGVT ERL+EGL+PRHCALSLVGEPIMYPEI
Sbjct: 346  WQWKMDDPLEIVNTAIDLHTKMIKQMKGVPGVTQERLTEGLTPRHCALSLVGEPIMYPEI 405

Query: 1167 NTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFWE 988
            N LVDELH+RRISTFLVTNAQFP+KI  LKPITQLYVSVDAA KDS+KAIDRPLFGDFWE
Sbjct: 406  NMLVDELHRRRISTFLVTNAQFPDKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 465

Query: 987  RFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSATS 808
            RF+DSLKAL+DKQQRTVYRLTLVKGWNTE+ DAYS LFSIG PDFVEIKGVTYCG+SATS
Sbjct: 466  RFIDSLKALRDKQQRTVYRLTLVKGWNTEDADAYSKLFSIGKPDFVEIKGVTYCGSSATS 525

Query: 807  KLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWIDY 628
            KLTMENVPWH+DVK FSEALA KS GEYEVACEH HSCCVLLAK DKFKV+GQW+TWIDY
Sbjct: 526  KLTMENVPWHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTDKFKVNGQWYTWIDY 585

Query: 627  EKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            EKFHDLVASG+PFDS DYMALTPSWAVYGA EGGFDPDQ+RY+KER+HK+
Sbjct: 586  EKFHDLVASGEPFDSADYMALTPSWAVYGAEEGGFDPDQSRYRKERHHKS 635


>gb|EOY14757.1| Flavodoxin family protein / radical SAM domain-containing protein
            isoform 1 [Theobroma cacao]
          Length = 637

 Score =  847 bits (2188), Expect = 0.0
 Identities = 416/590 (70%), Positives = 466/590 (78%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+I++T TSK LA+ L  +L++ N+ FDL++P+ YEPEDL  E L+LIIASTW+DG  P
Sbjct: 48   LFFITQTGTSKALAQRLLNLLSSNNIPFDLVDPQNYEPEDLPKESLILIIASTWEDGNPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
                F  NWL E  +DFR G+             GSR YGE  FNAV +     ++ LGA
Sbjct: 108  QGSKFFVNWLAEISTDFRAGNLLLSSCKFAVFGVGSRVYGET-FNAVARNLGKRLRGLGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E++ +            VF +W E VVRVLK G++   +N                   
Sbjct: 167  TEMVAVGEGDVDGGALESVFKEWSEKVVRVLKGGLVVENENGIVYASDAESFESDDDDNG 226

Query: 1707 XXXXXXGIVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIGS 1528
                   IVDLEDIAGK P++K         NGK +G +EMVTPVIRA+L KQGYKIIGS
Sbjct: 227  GGGGEEEIVDLEDIAGKGPSRKK-SVNVAETNGKMDGKREMVTPVIRANLEKQGYKIIGS 285

Query: 1527 HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 1348
            HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS
Sbjct: 286  HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 345

Query: 1347 WQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPEI 1168
            WQW+MDDPL IVN+AID H KMIKQMKGVPGVT ERL+EGL+PRHCALSLVGEPIMYPEI
Sbjct: 346  WQWKMDDPLEIVNTAIDLHTKMIKQMKGVPGVTQERLTEGLTPRHCALSLVGEPIMYPEI 405

Query: 1167 NTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFWE 988
            N LVDELH+RRISTFLVTNAQFP+KI  LKPITQLYVSVDAA KDS+KAIDRPLFGDFWE
Sbjct: 406  NMLVDELHRRRISTFLVTNAQFPDKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWE 465

Query: 987  RFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSATS 808
            RF+DSLKAL+DKQQRTVYRLTLVKGWNTE+ DAYS LFSIG PDFVEIKGVTYCG+SATS
Sbjct: 466  RFIDSLKALRDKQQRTVYRLTLVKGWNTEDADAYSKLFSIGKPDFVEIKGVTYCGSSATS 525

Query: 807  KLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWIDY 628
            KLTMENVPWH+DVK FSEALA KS GEYEVACEH HSCCVLLAK DKFKV+GQW+TWIDY
Sbjct: 526  KLTMENVPWHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTDKFKVNGQWYTWIDY 585

Query: 627  EKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKT 478
            EKFHDLVASG+PFDS DYMALTPSWAVYGA EGGFDPDQ+RY+KER+HK+
Sbjct: 586  EKFHDLVASGEPFDSADYMALTPSWAVYGAEEGGFDPDQSRYRKERHHKS 635


>ref|XP_022997485.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Cucurbita maxima]
          Length = 639

 Score =  847 bits (2188), Expect = 0.0
 Identities = 420/592 (70%), Positives = 466/592 (78%), Gaps = 1/592 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  L  +L +  L FD+++P+ +EPEDL  E LVL +ASTW+DG  P
Sbjct: 48   LFFISQTGTSKALAHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             +  FL+NWL+ES  DFRVGS             GSR+YGE  FNAV + FS  MKALGA
Sbjct: 108  PHAKFLANWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGET-FNAVARDFSKRMKALGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+L +             F  W   +++VLK   +  G +                   
Sbjct: 167  KEILAVGEGDVDGGDIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDE 226

Query: 1707 XXXXXXG-IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIG 1531
                    IVDLEDIAGK P++K      V  NGK NG K MVTPVIRASL KQGYKIIG
Sbjct: 227  DADPGEEDIVDLEDIAGKGPSRKSTN--VVETNGKLNGKKVMVTPVIRASLEKQGYKIIG 284

Query: 1530 SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 1351
            SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 285  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 344

Query: 1350 SWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPE 1171
            SWQW+MDDPL IVNSAID H KMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPE
Sbjct: 345  SWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPE 404

Query: 1170 INTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFW 991
            INTLVDELH+RRISTFLVTNAQFP+KI SLKP+TQLYVSVDAA K+S+KAIDRPLFGDFW
Sbjct: 405  INTLVDELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFW 464

Query: 990  ERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSAT 811
            ERF+DSLKAL DKQQRTVYRLTLVKGWNTE++DAYS+LFS+G PDFVEIKGVTYCG+S T
Sbjct: 465  ERFIDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNT 524

Query: 810  SKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWID 631
            SKLTMENVPWH+DVK FSEALA KS GEYEVACEHVHSCCVLLAK DKF+VDGQW TWID
Sbjct: 525  SKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWID 584

Query: 630  YEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKTK 475
            YEKFHDLVASGKPFDS DYMA TPSWAVYGA+EGGFDP Q+RY+KER+H+ K
Sbjct: 585  YEKFHDLVASGKPFDSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPK 636


>ref|XP_003550379.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Glycine max]
 gb|KRH05815.1| hypothetical protein GLYMA_17G250400 [Glycine max]
          Length = 646

 Score =  847 bits (2188), Expect = 0.0
 Identities = 419/629 (66%), Positives = 477/629 (75%), Gaps = 2/629 (0%)
 Frame = -1

Query: 2358 YCLYKSYSLRXXXXXXXXXXXXXXXXXXXXXXXXXXKIFYISETNTSKNLAKHLHKILTT 2179
            YCLYKS  LR                           I ++SET T+K LA+ LH++L++
Sbjct: 26   YCLYKSRRLRHLKLSLNPNPNPNPNPK----------ILFLSETGTAKTLARRLHRLLSS 75

Query: 2178 KNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSPSNGVFLSNWLNESVSDFRVGSXX 1999
             ++ FDL++ + YEPEDL  E L+L++ASTWQDG +P+   F + WL ++ +DFR GS  
Sbjct: 76   NDVAFDLVDAQHYEPEDLPKETLILLVASTWQDGAAPAASRFFATWLADAAADFRAGSLL 135

Query: 1998 XXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGAVEVLPICXXXXXXXXXXXV-FGK 1822
                       GSR+YG++ FNAV K  +  ++ALGA E +P+              F +
Sbjct: 136  LSRCRFAVFGVGSRAYGDDSFNAVAKGLAMHLRALGATEAVPLSEGDIDGGDDVDAVFDR 195

Query: 1821 WCEDVVRVLKS-GVLENGKNXXXXXXXXXXXXXXXXXXXXXXXXXGIVDLEDIAGKAPTK 1645
            WCE VV VLK  GV+E+  N                          IVDLEDIAGKAP++
Sbjct: 196  WCEKVVAVLKGVGVVESCDNDGAEECGVVSSSEEESDVESSE----IVDLEDIAGKAPSR 251

Query: 1644 KDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1465
            + +       NGK NG +EMVTPVIRA+L KQGYKIIGSHSGVK+CRWTK+QLRGRGGCY
Sbjct: 252  RKVVANGEESNGKLNGSREMVTPVIRANLEKQGYKIIGSHSGVKLCRWTKAQLRGRGGCY 311

Query: 1464 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWQMDDPLMIVNSAIDQHKK 1285
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWQMDDP+ IVNSAID H  
Sbjct: 312  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWQMDDPIEIVNSAIDLHTN 371

Query: 1284 MIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRISTFLVTNAQ 1105
            MIKQMKGVPGVT ERLSEGLSPRHCALSLVGEPIMYPEIN LVDELH+RRISTFLVTNAQ
Sbjct: 372  MIKQMKGVPGVTLERLSEGLSPRHCALSLVGEPIMYPEINALVDELHKRRISTFLVTNAQ 431

Query: 1104 FPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFWERFVDSLKALKDKQQRTVYRLT 925
            FPEKI SLKPITQLYVSVDAA KDS+KAIDRPLFGDFWERF+DSL ALK+K QRTVYRLT
Sbjct: 432  FPEKIKSLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLTALKEKHQRTVYRLT 491

Query: 924  LVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSATSKLTMENVPWHADVKEFSEALA 745
            LVKGWNTEEVDAYS LFS+G PDFVEIKGVTYCG+S TSKLTM+NVPWHADVK FSEALA
Sbjct: 492  LVKGWNTEEVDAYSKLFSLGEPDFVEIKGVTYCGSSTTSKLTMDNVPWHADVKAFSEALA 551

Query: 744  QKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWIDYEKFHDLVASGKPFDSNDYMAL 565
             KS GEYEVACEH HSCCVLLAK +KFK+DGQW+TWIDYEKFHDLVASG+ FDS DYMA 
Sbjct: 552  LKSQGEYEVACEHAHSCCVLLAKTNKFKIDGQWYTWIDYEKFHDLVASGRTFDSRDYMAA 611

Query: 564  TPSWAVYGANEGGFDPDQARYKKERNHKT 478
            TPSW+VYG+ EGGFDP Q RY+KER+HK+
Sbjct: 612  TPSWSVYGSEEGGFDPGQLRYRKERHHKS 640


>ref|XP_022962153.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Cucurbita moschata]
          Length = 639

 Score =  846 bits (2185), Expect = 0.0
 Identities = 419/592 (70%), Positives = 466/592 (78%), Gaps = 1/592 (0%)
 Frame = -1

Query: 2247 IFYISETNTSKNLAKHLHKILTTKNLKFDLINPETYEPEDLNTEDLVLIIASTWQDGLSP 2068
            +F+IS+T TSK LA  L  +L +  L FD+++P+ +EPEDL  E LVL +ASTW+DG  P
Sbjct: 48   LFFISQTGTSKALAHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPP 107

Query: 2067 SNGVFLSNWLNESVSDFRVGSXXXXXXXXXXXXXGSRSYGEERFNAVGKAFSSGMKALGA 1888
             +  FL++WL+ES  DFRVGS             GSR+YGE  FNAV + FS  MKALGA
Sbjct: 108  PHAKFLASWLSESAEDFRVGSLLLSQCKFAVFGVGSRAYGET-FNAVARDFSKRMKALGA 166

Query: 1887 VEVLPICXXXXXXXXXXXVFGKWCEDVVRVLKSGVLENGKNXXXXXXXXXXXXXXXXXXX 1708
             E+L +             F  W   +++VLK   +  G +                   
Sbjct: 167  KEILAVGEGDVDGGDIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESVEEDDE 226

Query: 1707 XXXXXXG-IVDLEDIAGKAPTKKDLEKGAVARNGKKNGVKEMVTPVIRASLTKQGYKIIG 1531
                    IVDLEDIAGK P++K      V  NGK NG K MVTPVIRASL KQGYKIIG
Sbjct: 227  DADPGEEDIVDLEDIAGKGPSRKSTN--VVETNGKLNGKKVMVTPVIRASLEKQGYKIIG 284

Query: 1530 SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 1351
            SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK
Sbjct: 285  SHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK 344

Query: 1350 SWQWQMDDPLMIVNSAIDQHKKMIKQMKGVPGVTAERLSEGLSPRHCALSLVGEPIMYPE 1171
            SWQW+MDDPL IVNSAID HKKMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPE
Sbjct: 345  SWQWEMDDPLDIVNSAIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPE 404

Query: 1170 INTLVDELHQRRISTFLVTNAQFPEKIISLKPITQLYVSVDAANKDSMKAIDRPLFGDFW 991
            INTLVDELH+RRISTFLVTNAQFP+KI SLKP+TQLYVSVDAA KDS+KAIDRPLFGDFW
Sbjct: 405  INTLVDELHRRRISTFLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFW 464

Query: 990  ERFVDSLKALKDKQQRTVYRLTLVKGWNTEEVDAYSSLFSIGNPDFVEIKGVTYCGTSAT 811
            ERF+DSLKAL DKQQRTVYRLTLVKGWNTE++DAYS+LFS+G PDFVEIKGVTYCG+S T
Sbjct: 465  ERFIDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNT 524

Query: 810  SKLTMENVPWHADVKEFSEALAQKSNGEYEVACEHVHSCCVLLAKVDKFKVDGQWHTWID 631
            SKLTMENVPWH+DVK FSEALA KS GEYEVACEHVHSCCVLLAK DKF++DGQW TWID
Sbjct: 525  SKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWID 584

Query: 630  YEKFHDLVASGKPFDSNDYMALTPSWAVYGANEGGFDPDQARYKKERNHKTK 475
            YEKFH LVASGKPFDS DYMA TPSWAVYGA+EGGFDP Q+RY+KER+H+ K
Sbjct: 585  YEKFHALVASGKPFDSKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPK 636