BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00014295
         (1742 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_022018796.1| threonylcarbamoyladenosine tRNA methylthiotr...   946   0.0  
ref|XP_023768214.1| threonylcarbamoyladenosine tRNA methylthiotr...   912   0.0  
ref|XP_017253873.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   828   0.0  
ref|XP_012446896.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   820   0.0  
ref|XP_021288193.1| threonylcarbamoyladenosine tRNA methylthiotr...   818   0.0  
ref|XP_006350322.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   818   0.0  
ref|XP_016686903.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   817   0.0  
gb|KHG16846.1| CDK5 regulatory subunit-associated protein 1-like...   817   0.0  
ref|XP_004250429.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   816   0.0  
ref|XP_017606144.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   816   0.0  
ref|XP_006376236.1| radical SAM domain-containing family protein...   813   0.0  
gb|KJB60078.1| hypothetical protein B456_009G288500 [Gossypium r...   810   0.0  
ref|XP_016496803.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   812   0.0  
ref|XP_015058303.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   813   0.0  
ref|XP_009781017.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   812   0.0  
ref|XP_016438122.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   813   0.0  
ref|XP_016748500.1| PREDICTED: threonylcarbamoyladenosine tRNA m...   813   0.0  
ref|XP_007020083.2| PREDICTED: threonylcarbamoyladenosine tRNA m...   812   0.0  
gb|PNT07772.1| hypothetical protein POPTR_013G107800v3 [Populus ...   811   0.0  
gb|EOY17308.1| Methylthiotransferase [Theobroma cacao]                811   0.0  

>ref|XP_022018796.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Helianthus
            annuus]
 gb|OTG34298.1| putative methylthiotransferase [Helianthus annuus]
          Length = 599

 Score =  946 bits (2444), Expect = 0.0
 Identities = 477/542 (88%), Positives = 499/542 (92%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLS+FGYA+SD+AEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG
Sbjct: 62   SDSEYMAGQLSSFGYAISDDAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 121

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGS+DLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF
Sbjct: 122  CVPQGSKDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 181

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVV+DGVKEIWLSSEDTGAYG
Sbjct: 182  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVDDGVKEIWLSSEDTGAYG 241

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVAALP +GSTMLRIGMTNPPYILEHLKEIA IL HPCVYSFLHVPV
Sbjct: 242  RDIGVNLPILLNAIVAALPTNGSTMLRIGMTNPPYILEHLKEIAHILCHPCVYSFLHVPV 301

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK
Sbjct: 302  QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 361

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEG +ERIWI
Sbjct: 362  EYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGKIERIWI 421

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIATD+IHLVGHTKGYVQVLVVGP+SLLGSSA++KITSVGRWSVFGDVIEIL++S+D  
Sbjct: 422  TDIATDQIHLVGHTKGYVQVLVVGPDSLLGSSAMVKITSVGRWSVFGDVIEILTESSDTA 481

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
            IPSD+K+  N ER SPC NQED C CS  KPEPCACESDAC SN CN I      QN   
Sbjct: 482  IPSDDKDTINKERSSPCGNQEDACVCSPAKPEPCACESDACGSNCCN-IAANDPAQNGPT 540

Query: 300  DSSLLQRRKPSPATVEKEDNSHQKLATQSVRDGGWALLDTALLGGIFISLMTIVALVLYA 121
            + SLL RRKPSPA VE E NS+QKLA  +V    W LLDTAL+GGIFIS +TIVAL LY 
Sbjct: 541  NPSLLLRRKPSPAMVETEGNSNQKLAAGNVH---WGLLDTALIGGIFISFLTIVALFLYV 597

Query: 120  RS 115
             S
Sbjct: 598  GS 599


>ref|XP_023768214.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Lactuca
            sativa]
 gb|PLY82124.1| hypothetical protein LSAT_1X12201 [Lactuca sativa]
          Length = 611

 Score =  912 bits (2357), Expect = 0.0
 Identities = 461/546 (84%), Positives = 495/546 (90%), Gaps = 5/546 (0%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLS+FGYA+SDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG
Sbjct: 71   SDSEYMAGQLSSFGYAISDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 130

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLP+LDLPKVRKNKF
Sbjct: 131  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPSLDLPKVRKNKF 190

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVV+DGVKEIWLSSEDTGAYG
Sbjct: 191  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVDDGVKEIWLSSEDTGAYG 250

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIV ALP +GSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV
Sbjct: 251  RDIGVNLPILLNAIVGALPSNGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 310

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAM REYT GEFR VVDTLTELVPGMQIATDIICGFPGETDEDFA+TV+LIK
Sbjct: 311  QSGSDAILTAMKREYTAGEFRMVVDTLTELVPGMQIATDIICGFPGETDEDFAQTVDLIK 370

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKVASN+VKKRSRELTSVFESFTPYNGMEGNMERIWI
Sbjct: 371  EYKFSQVHISQFYPRPGTPAARMKKVASNIVKKRSRELTSVFESFTPYNGMEGNMERIWI 430

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSND-L 484
            TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAI+KITSVGRWSVFGDVI+IL +S   +
Sbjct: 431  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIVKITSVGRWSVFGDVIKILDESQGVV 490

Query: 483  KIPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNN 316
            K  +  KENC+    SPC +Q+D CACS  KPE CAC S+ C     +N+ ++ PE Q  
Sbjct: 491  KKDTSNKENCSP---SPCFDQQDACACSEAKPESCACGSNGCDIDANANANDSYPEAQKA 547

Query: 315  QNALNDSSLLQRRKPSPATVEKEDNSHQKLATQSVRDGGWALLDTALLGGIFISLMTIVA 136
            QN     SLL RRKPS  TVE EDN +QK+AT +++D  W ++D  L+GGIF+SL+T+VA
Sbjct: 548  QNI---PSLLLRRKPSLTTVEMEDNGNQKVATSNMQDRDWGVVDKLLVGGIFLSLLTMVA 604

Query: 135  LVLYAR 118
            L+LY R
Sbjct: 605  LILYVR 610


>ref|XP_017253873.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Daucus carota subsp. sativus]
 gb|KZM94122.1| hypothetical protein DCAR_017367 [Daucus carota subsp. sativus]
          Length = 620

 Score =  828 bits (2139), Expect = 0.0
 Identities = 426/552 (77%), Positives = 471/552 (85%), Gaps = 10/552 (1%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLS+FGYA+SDNA+ ADLWLINTCTVKSPSQSAMDTLIS+CK+AKKPLVVAG
Sbjct: 67   SDSEYMAGQLSSFGYALSDNADEADLWLINTCTVKSPSQSAMDTLISRCKTAKKPLVVAG 126

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRD+KELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF
Sbjct: 127  CVPQGSRDIKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 186

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRV+SVV DGVKEIWLSSEDTGAYG
Sbjct: 187  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVRSVVADGVKEIWLSSEDTGAYG 246

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIA++LRHPCVYSFLHVPV
Sbjct: 247  RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIADVLRHPCVYSFLHVPV 306

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAIL+AMNREYTVG+FR VVDTLTELVPGMQIATDIICGFPGETDEDFA+TVNLIK
Sbjct: 307  QSGSDAILSAMNREYTVGDFRTVVDTLTELVPGMQIATDIICGFPGETDEDFAQTVNLIK 366

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYKF QVHISQFYPRPGTPAARMKKV S  VKKRSRELTSVFESFTPYNGMEG +ERIWI
Sbjct: 367  DYKFPQVHISQFYPRPGTPAARMKKVPSTSVKKRSRELTSVFESFTPYNGMEGRIERIWI 426

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IATD  HLVGHTKGY+QVLVVGP S+LG+SAII+ITSVGRWSVFG+VIE L+ + D  
Sbjct: 427  TEIATDGKHLVGHTKGYMQVLVVGPGSMLGTSAIIRITSVGRWSVFGEVIETLNSTKD-N 485

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC--------KSNSCNAIPET 325
            + S E+ +  +E C   SN E++CAC+ E  + CAC  ++C           S +     
Sbjct: 486  VESKERSD-RSENCCSLSNLEESCACAGEL-DACACGHESCGGLNLTEENGGSLSKSGSW 543

Query: 324  QNNQNALNDSSLLQRRKPSPATVEKEDNSHQKLATQSVRDGG--WALLDTALLGGIFISL 151
             ++++ LN    L R++ +  T + ED+S Q +A Q    G   W  +D  LLGGI  S 
Sbjct: 544  ADDRSNLNVFGWLLRKRKNHQTTKLEDDSLQHIARQESAAGAHMWTAVDRLLLGGICASF 603

Query: 150  MTIVALVLYARS 115
            +TI+AL LY  S
Sbjct: 604  LTILALFLYFAS 615


>ref|XP_012446896.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Gossypium raimondii]
 gb|KJB60079.1| hypothetical protein B456_009G288500 [Gossypium raimondii]
          Length = 617

 Score =  820 bits (2119), Expect = 0.0
 Identities = 423/562 (75%), Positives = 468/562 (83%), Gaps = 16/562 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVK+PSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 67   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKNPSQSAMNTLITKCKSAKKPLVVAG 126

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF
Sbjct: 127  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 186

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 187  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVKTVIADGVREIWLSSEDTGAYG 246

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLP+LLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIA +L HPCVYSFLHVPV
Sbjct: 247  RDIGVNLPVLLNAIVAELPPDGSTMLRIGMTNPPFILEHLKEIAAVLCHPCVYSFLHVPV 306

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDA+LTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDF++TVNLIK
Sbjct: 307  QSGSDAVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVNLIK 366

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 367  EYKFSQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGKVERIWI 426

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IATD IHLVGHTKGYVQVL+V PE++LG+SAI+KITSVGRWSVFG+V+E L + N   
Sbjct: 427  TEIATDGIHLVGHTKGYVQVLIVAPETILGTSAIVKITSVGRWSVFGEVLETLPQVNVKM 486

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNNQ 313
             PS   +  N E+ SPCSNQ D+CACS E PEPC+C  D C    K + C          
Sbjct: 487  APS---KVSNQEKHSPCSNQYDSCACSQE-PEPCSCGPDICGGQIKLDKCTV-------- 534

Query: 312  NALNDSSLLQRRKPSPA--TVEKEDNSHQK-------LATQSVRD---GGWALLDTALLG 169
             + NDS +  R   +P    V K  N  QK       L  ++ +D   G W  +D ALL 
Sbjct: 535  -SKNDSPMENRASKNPIHWFVRKRRNHAQKKVENDIALGLENEQDWAPGSWGFVDRALLV 593

Query: 168  GIFISLMTIVALVLYARS*TLI 103
            GIF+S + IVA++++    TL+
Sbjct: 594  GIFVSFLMIVAVIMHLEFRTLL 615


>ref|XP_021288193.1| threonylcarbamoyladenosine tRNA methylthiotransferase [Herrania
            umbratica]
          Length = 623

 Score =  818 bits (2114), Expect = 0.0
 Identities = 417/556 (75%), Positives = 468/556 (84%), Gaps = 10/556 (1%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVKSPSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 73   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKSPSQSAMNTLITKCKSAKKPLVVAG 132

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL+NRKTLPALDLPKVRKNKF
Sbjct: 133  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLMNRKTLPALDLPKVRKNKF 192

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYT+DSLVGRVK+V+ DGVKEIWLSSEDTGAYG
Sbjct: 193  VEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVGRVKTVIADGVKEIWLSSEDTGAYG 252

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DG+TMLRIGMTNPP+ILEHLKEI  +LRHPCVYSFLHVPV
Sbjct: 253  RDIGVNLPILLNAIVAELPSDGNTMLRIGMTNPPFILEHLKEIGNVLRHPCVYSFLHVPV 312

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAMNREYTV EFR VVDTLTELVP MQIATDIICGFPGETDEDFA+TVNLIK
Sbjct: 313  QSGSDAILTAMNREYTVTEFRTVVDTLTELVPEMQIATDIICGFPGETDEDFAQTVNLIK 372

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKF QVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 373  EYKFPQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGRVERIWI 432

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IA D IHLVGHTKGYVQVLV+ PES+LG+SAI+KITSVGRWSVFG+VIE L++ N   
Sbjct: 433  TEIAADGIHLVGHTKGYVQVLVIAPESMLGTSAIVKITSVGRWSVFGEVIETLTQVNLKM 492

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
             P   ++ CN E+CSP  NQ D+CACS E PEPC+C  ++C   +        +N + + 
Sbjct: 493  AP---EKVCNQEKCSPRFNQFDSCACSKE-PEPCSCGPNSCGGQTLLEECTVSSNDSWME 548

Query: 300  DSS-------LLQRRKPSPATVEKEDNSHQKLATQSVRD---GGWALLDTALLGGIFISL 151
            D S       LL++R+       K+  +   L  +  +D   GGW ++D ALL GI +S 
Sbjct: 549  DRSSKNLIGWLLRKRRNHE---HKKVENGIALGLRKKQDWALGGWGIVDRALLVGILVSF 605

Query: 150  MTIVALVLYARS*TLI 103
            + IVA++++    T++
Sbjct: 606  LMIVAVIIHLEFRTIL 621


>ref|XP_006350322.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            isoform X1 [Solanum tuberosum]
          Length = 622

 Score =  818 bits (2113), Expect = 0.0
 Identities = 421/549 (76%), Positives = 474/549 (86%), Gaps = 11/549 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN + ADLW+INTCTVKSPSQSAMDT+ISK +SAKKPLVVAG
Sbjct: 71   SDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSAMDTIISKGRSAKKPLVVAG 130

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL RKTLPALDLPKVRKNKF
Sbjct: 131  CVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKF 190

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 191  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNVIADGVREIWLSSEDTGAYG 250

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LPL+GSTMLRIGMTNPPYILEHLKEIA++L HPCVYSFLHVPV
Sbjct: 251  RDIGVNLPILLNAIVAELPLNGSTMLRIGMTNPPYILEHLKEIADVLCHPCVYSFLHVPV 310

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSD+IL+AMNREYTVGEFRKVVDTL ELVPGMQIATDIICGFPGETDEDFA+TV+LIK
Sbjct: 311  QSGSDSILSAMNREYTVGEFRKVVDTLMELVPGMQIATDIICGFPGETDEDFAQTVDLIK 370

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYK +QVHISQFYPRPGTPAARMKKV SNVVK+RSRELT+VFESFTPY G+EG +ERIWI
Sbjct: 371  DYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAVFESFTPYTGVEGKVERIWI 430

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIA+D IHLVGHTKGY+QVLV+GPES++GSSA++KITSVGRWSVFG+VIEIL ++N+  
Sbjct: 431  TDIASDGIHLVGHTKGYIQVLVIGPESMMGSSAMVKITSVGRWSVFGEVIEILFQNNE-D 489

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC-KSNSCNAIPETQNNQNAL 304
            +PS    +   ERCSPC+  E+ CACS E+ EPC+  SD C +S +  A    +N+ N  
Sbjct: 490  VPSHRSFD---ERCSPCALSEEDCACSKEQ-EPCSATSDCCNQSPTREATTLLKNDPNQD 545

Query: 303  NDSS------LLQRRKPSPATVEKEDNSHQKLATQSVRD----GGWALLDTALLGGIFIS 154
            N SS       L+ RK   +  +K D +  +   + ++D      W+L+D ALL GI +S
Sbjct: 546  NHSSRNLIGWFLRNRKNQSS--KKMDLTRSESKEKQIQDLSHRSAWSLVDIALLSGILLS 603

Query: 153  LMTIVALVL 127
            L TIVAL L
Sbjct: 604  LFTIVALFL 612


>ref|XP_016686903.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase-like
            [Gossypium hirsutum]
          Length = 617

 Score =  817 bits (2110), Expect = 0.0
 Identities = 422/562 (75%), Positives = 470/562 (83%), Gaps = 16/562 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVK+PSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 67   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKNPSQSAMNTLITKCKSAKKPLVVAG 126

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF
Sbjct: 127  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 186

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 187  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVKTVIADGVREIWLSSEDTGAYG 246

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIA +L HPCVYSFLHVPV
Sbjct: 247  RDIGVNLPILLNAIVAELPPDGSTMLRIGMTNPPFILEHLKEIAAVLCHPCVYSFLHVPV 306

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDA+LTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETD+DF++TVNLIK
Sbjct: 307  QSGSDAVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDDDFSQTVNLIK 366

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 367  EYKFSQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGKVERIWI 426

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IATD IHLVGHTKGYVQVL+V PE++LG++AI+KITSVGRWSVFG+V+E L + N +K
Sbjct: 427  TEIATDGIHLVGHTKGYVQVLIVAPETILGTAAIVKITSVGRWSVFGEVLETLPQVN-VK 485

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNNQ 313
            +  D+    N E+ SPCSNQ D+CACS E PEPC+C  D C    K + C          
Sbjct: 486  MAPDKVS--NQEKHSPCSNQYDSCACSKE-PEPCSCGPDICGGQMKLDKCAV-------- 534

Query: 312  NALNDSSLLQRRKPSPA--TVEKEDNSHQK-------LATQSVRD---GGWALLDTALLG 169
             + NDS +  R   +P    V K  N  QK       L  ++ +D   G W  +D ALL 
Sbjct: 535  -SKNDSPMENRASKNPIHWFVRKRRNHAQKKVENDIALGLENEQDWAPGSWGFVDRALLV 593

Query: 168  GIFISLMTIVALVLYARS*TLI 103
            GIF+S + IVA++++    TL+
Sbjct: 594  GIFVSFLMIVAVIMHLEFRTLL 615


>gb|KHG16846.1| CDK5 regulatory subunit-associated protein 1-like 1 [Gossypium
            arboreum]
          Length = 631

 Score =  817 bits (2111), Expect = 0.0
 Identities = 422/562 (75%), Positives = 469/562 (83%), Gaps = 16/562 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVK+PSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 81   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKNPSQSAMNTLITKCKSAKKPLVVAG 140

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLP+LDLPKVRKNKF
Sbjct: 141  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPSLDLPKVRKNKF 200

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 201  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVKTVIADGVREIWLSSEDTGAYG 260

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIA +L HPCVYSFLHVPV
Sbjct: 261  RDIGVNLPILLNAIVAELPPDGSTMLRIGMTNPPFILEHLKEIAAVLCHPCVYSFLHVPV 320

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDA+LTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDF++TVNLIK
Sbjct: 321  QSGSDAVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVNLIK 380

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 381  EYKFSQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGKVERIWI 440

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IATD IHLVGHTKGYVQVL+V PE++LG+SAI+KITSVGRWSVFG+V+E L + N +K
Sbjct: 441  TEIATDGIHLVGHTKGYVQVLIVAPETILGTSAIVKITSVGRWSVFGEVLESLPQVN-VK 499

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNNQ 313
            +  D+    N E+ SPCSNQ + CACS E PEPC+C  D C    K + C          
Sbjct: 500  MAPDKVS--NQEKHSPCSNQYECCACSKE-PEPCSCGPDICGGQIKLDKCTV-------- 548

Query: 312  NALNDSSLLQRRKPSPA--TVEKEDNSHQK-------LATQSVRD---GGWALLDTALLG 169
             + NDS +  R   +P    + K  N  QK       L  ++ +D   GGW  +D ALL 
Sbjct: 549  -SKNDSPMENRASKNPIHWFIRKRRNHAQKKLENDIALGLENEQDWAPGGWGFVDRALLV 607

Query: 168  GIFISLMTIVALVLYARS*TLI 103
            GIF+S   IVA++++    TL+
Sbjct: 608  GIFVSFFMIVAVIMHLEFRTLL 629


>ref|XP_004250429.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Solanum lycopersicum]
          Length = 622

 Score =  816 bits (2109), Expect = 0.0
 Identities = 418/547 (76%), Positives = 472/547 (86%), Gaps = 11/547 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN + ADLW+INTCTVKSPSQSAMDT+ISK +SAKKPLVVAG
Sbjct: 71   SDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSAMDTIISKGRSAKKPLVVAG 130

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR+LK+LEGVSIVGVQQIDRVVEVVEETLKGHEVRLL RKTLPALDLPKVRKNKF
Sbjct: 131  CVPQGSRNLKDLEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKF 190

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 191  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNVIADGVREIWLSSEDTGAYG 250

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LPL+GSTMLRIGMTNPPYILEHLKEIA++L HPCVYSFLHVPV
Sbjct: 251  RDIGVNLPILLNAIVAQLPLNGSTMLRIGMTNPPYILEHLKEIADVLCHPCVYSFLHVPV 310

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSD++L+AMNREYTVGEFRKVVDTL ELVPGMQIATDIICGFPGETDEDFA+TV+LIK
Sbjct: 311  QSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDIICGFPGETDEDFAQTVDLIK 370

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYK +QVHISQFYPRPGTPAARMKKV SNVVK+RSRELT+VFESFTPY G+EG +ERIWI
Sbjct: 371  DYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAVFESFTPYTGVEGKVERIWI 430

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TD+A+D +HLVGHTKGY+QVLV+GPES+LGSSA++KITSVGRWSVFG+VIEIL + ND  
Sbjct: 431  TDVASDGVHLVGHTKGYIQVLVIGPESMLGSSAMVKITSVGRWSVFGEVIEILCQ-NDKD 489

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC-KSNSCNAIPETQNNQNAL 304
            +PS    +   ERCSPC+  E+ CACS E+ EPC+  SD C +S +  A    +N+ N  
Sbjct: 490  VPSHRSFD---ERCSPCALSEEACACSKEQ-EPCSATSDCCNQSPTVEATTLLKNDPNLD 545

Query: 303  NDSS------LLQRRKPSPATVEKEDNSHQKLATQSVRD----GGWALLDTALLGGIFIS 154
            N SS       L+ RK   +  +K D +  +   + ++D      W+L+D ALL GI +S
Sbjct: 546  NHSSRNLIGWFLRNRKNQSS--KKMDLTSSESKEKPIQDLSHRSAWSLVDIALLSGILLS 603

Query: 153  LMTIVAL 133
            L TIVAL
Sbjct: 604  LFTIVAL 610


>ref|XP_017606144.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            isoform X2 [Gossypium arboreum]
          Length = 617

 Score =  816 bits (2108), Expect = 0.0
 Identities = 421/562 (74%), Positives = 469/562 (83%), Gaps = 16/562 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVK+PSQSAM+T+I+KCKSAKKPLVVAG
Sbjct: 67   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKNPSQSAMNTVITKCKSAKKPLVVAG 126

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLP+LDLPKVRKNKF
Sbjct: 127  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPSLDLPKVRKNKF 186

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 187  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVKTVIADGVREIWLSSEDTGAYG 246

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIA +L HPCVYSFLHVPV
Sbjct: 247  RDIGVNLPILLNAIVAELPPDGSTMLRIGMTNPPFILEHLKEIAAVLCHPCVYSFLHVPV 306

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDA+LTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDF++TVNLIK
Sbjct: 307  QSGSDAVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVNLIK 366

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 367  EYKFSQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGKVERIWI 426

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IATD IHLVGHTKGYVQVL+V PE++LG+SAI+KITSVGRWSVFG+V+E L + N +K
Sbjct: 427  TEIATDGIHLVGHTKGYVQVLIVAPETILGTSAIVKITSVGRWSVFGEVLESLPQVN-VK 485

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNNQ 313
            +  D+    N E+ SPCSNQ + CACS E PEPC+C  D C    K + C          
Sbjct: 486  MAPDKVS--NQEKHSPCSNQYECCACSKE-PEPCSCGPDICGGQIKLDKCTV-------- 534

Query: 312  NALNDSSLLQRRKPSPA--TVEKEDNSHQK-------LATQSVRD---GGWALLDTALLG 169
             + NDS +  R   +P    + K  N  QK       L  ++ +D   GGW  +D ALL 
Sbjct: 535  -SKNDSPMENRASKNPIHWFIRKRRNHAQKKLENDIALGLENEQDWAPGGWGFVDRALLV 593

Query: 168  GIFISLMTIVALVLYARS*TLI 103
            GIF+S   IVA++++    TL+
Sbjct: 594  GIFVSFFMIVAVIMHLEFRTLL 615


>ref|XP_006376236.1| radical SAM domain-containing family protein [Populus trichocarpa]
          Length = 612

 Score =  813 bits (2100), Expect = 0.0
 Identities = 416/552 (75%), Positives = 463/552 (83%), Gaps = 13/552 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLS+FGY++SD+ E ADLWLINTCTVKSPSQSAMDTLISK KSAKKPLVVAG
Sbjct: 58   SDSEYMAGQLSSFGYSLSDSPEEADLWLINTCTVKSPSQSAMDTLISKGKSAKKPLVVAG 117

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF
Sbjct: 118  CVPQGSRNVKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 177

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSL GRVK+V++DGVKEIWLSSEDTGAYG
Sbjct: 178  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLAGRVKTVIDDGVKEIWLSSEDTGAYG 237

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIAE+LRHPCVYSFLHVPV
Sbjct: 238  RDIGVNLPILLNAIVAELPSDGSTMLRIGMTNPPFILEHLKEIAEVLRHPCVYSFLHVPV 297

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETD+DF++TVNLIK
Sbjct: 298  QSGSDAILTAMNREYTVNEFRTVVDTLTELVPGMQIATDIICGFPGETDKDFSQTVNLIK 357

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
             YKF+QVHISQFYPRPGTPAARMKKV SN+VK+RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 358  AYKFAQVHISQFYPRPGTPAARMKKVPSNIVKQRSRELTSVFEAFTPYNGMEGRVERIWI 417

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIA D IHLVGHTK YVQVL+V  ES+LG+SAI+KITSVGRWSVFG+VIE L++ N  K
Sbjct: 418  TDIAADGIHLVGHTKAYVQVLIVAQESMLGTSAIVKITSVGRWSVFGEVIETLNQINQ-K 476

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
              S EK   + E+CSPCS+  D+CACS E  EPCAC  ++C   S     +   N+    
Sbjct: 477  SKSVEK-MLSEEKCSPCSDPCDSCACSGES-EPCACGPESCGGQSTIEQSDVLQNEVLRE 534

Query: 300  DSSLLQRRKPSPATVEKEDNSHQKLATQSVRDGG-------------WALLDTALLGGIF 160
            D +   RR      + K  N  QK+    +  G              W ++D ALLGG+ 
Sbjct: 535  DQN---RRNLIGWLLRKRKNQAQKMVENGIASGSQKKQEWAKGAPEEWGVVDRALLGGLI 591

Query: 159  ISLMTIVALVLY 124
            +S+ T VA++++
Sbjct: 592  VSVFTTVAILIH 603


>gb|KJB60078.1| hypothetical protein B456_009G288500 [Gossypium raimondii]
          Length = 546

 Score =  810 bits (2093), Expect = 0.0
 Identities = 418/557 (75%), Positives = 463/557 (83%), Gaps = 16/557 (2%)
 Frame = -3

Query: 1725 MAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAGCVPQG 1546
            MAGQLSAFGYA+SDN E  DLWLINTCTVK+PSQSAM+TLI+KCKSAKKPLVVAGCVPQG
Sbjct: 1    MAGQLSAFGYAISDNPEEGDLWLINTCTVKNPSQSAMNTLITKCKSAKKPLVVAGCVPQG 60

Query: 1545 SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFVEILP 1366
            SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFVEILP
Sbjct: 61   SRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKFVEILP 120

Query: 1365 INVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYGRDIGV 1186
            INVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYGRDIGV
Sbjct: 121  INVGCLGACTYCKTKHARGHLGSYTVDSLVERVKTVIADGVREIWLSSEDTGAYGRDIGV 180

Query: 1185 NLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPVQSGSD 1006
            NLP+LLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIA +L HPCVYSFLHVPVQSGSD
Sbjct: 181  NLPVLLNAIVAELPPDGSTMLRIGMTNPPFILEHLKEIAAVLCHPCVYSFLHVPVQSGSD 240

Query: 1005 AILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIKDYKFS 826
            A+LTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDF++TVNLIK+YKFS
Sbjct: 241  AVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVNLIKEYKFS 300

Query: 825  QVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWITDIAT 646
            QVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWIT+IAT
Sbjct: 301  QVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGKVERIWITEIAT 360

Query: 645  DEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLKIPSDE 466
            D IHLVGHTKGYVQVL+V PE++LG+SAI+KITSVGRWSVFG+V+E L + N    PS  
Sbjct: 361  DGIHLVGHTKGYVQVLIVAPETILGTSAIVKITSVGRWSVFGEVLETLPQVNVKMAPS-- 418

Query: 465  KENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNNQNALND 298
             +  N E+ SPCSNQ D+CACS E PEPC+C  D C    K + C           + ND
Sbjct: 419  -KVSNQEKHSPCSNQYDSCACSQE-PEPCSCGPDICGGQIKLDKCTV---------SKND 467

Query: 297  SSLLQRRKPSPA--TVEKEDNSHQK-------LATQSVRD---GGWALLDTALLGGIFIS 154
            S +  R   +P    V K  N  QK       L  ++ +D   G W  +D ALL GIF+S
Sbjct: 468  SPMENRASKNPIHWFVRKRRNHAQKKVENDIALGLENEQDWAPGSWGFVDRALLVGIFVS 527

Query: 153  LMTIVALVLYARS*TLI 103
             + IVA++++    TL+
Sbjct: 528  FLMIVAVIMHLEFRTLL 544


>ref|XP_016496803.1| PREDICTED: threonylcarbamoyladenosine tRNA
            methylthiotransferase-like, partial [Nicotiana tabacum]
          Length = 587

 Score =  812 bits (2097), Expect = 0.0
 Identities = 414/551 (75%), Positives = 470/551 (85%), Gaps = 13/551 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN + ADLW+INTCTVKSPSQSAMDTLISK +SAKKPLVVAG
Sbjct: 34   SDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSAMDTLISKGRSAKKPLVVAG 93

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL RKTLPALDLPKVRKNKF
Sbjct: 94   CVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKF 153

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 154  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNVIADGVREIWLSSEDTGAYG 213

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LPLDGSTMLRIGMTNPPYILEHLKEIA++LRHPCVY+FLHVPV
Sbjct: 214  RDIGVNLPILLNAIVAELPLDGSTMLRIGMTNPPYILEHLKEIADVLRHPCVYTFLHVPV 273

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSD++L+AMNREYTVGEFRKVVDTL ELVPGMQIATDIICGFPGETDEDF +TV+LIK
Sbjct: 274  QSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDIICGFPGETDEDFGQTVDLIK 333

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYK +QVHISQFYPRPGTPAARMKKV SNVVK+RSR+LT+VFESFTPY GMEG +ERIWI
Sbjct: 334  DYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRKLTAVFESFTPYTGMEGKVERIWI 393

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIA+D IHLVGHTKGY+QVLV+GPES+LG+SA++KITSVGRWSVFG+VIEI+S +N  +
Sbjct: 394  TDIASDGIHLVGHTKGYIQVLVIGPESMLGTSAMVKITSVGRWSVFGEVIEIISHNNK-E 452

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPET-------- 325
            + S EK +   E+CSPC+N E+ CACS E+    +C + +C S S   + ET        
Sbjct: 453  VGSHEKVD---EKCSPCANSEEACACSKEQELGSSCGTTSCCSQS--PMQETTTLLKNEP 507

Query: 324  -QNNQNALNDSSLLQRRKPSPATVEKE----DNSHQKLATQSVRDGGWALLDTALLGGIF 160
             Q++Q + N      R + + ++ + +    ++  +K      R   W  +D ALL GI 
Sbjct: 508  KQDDQGSRNLIGWFLRNRKNHSSKKMDLAGLESKEKKQIQDLGRQSAWGPVDIALLSGIL 567

Query: 159  ISLMTIVALVL 127
            +SL TIVAL L
Sbjct: 568  LSLFTIVALFL 578


>ref|XP_015058303.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Solanum pennellii]
          Length = 622

 Score =  813 bits (2100), Expect = 0.0
 Identities = 418/547 (76%), Positives = 471/547 (86%), Gaps = 11/547 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN + ADLW+INTCTVKSPSQSAMDT+ISK +SAKKPLVVAG
Sbjct: 71   SDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSAMDTIISKGRSAKKPLVVAG 130

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL RKTLPALDLPKVRKNKF
Sbjct: 131  CVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKF 190

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 191  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVCRVKNVIADGVREIWLSSEDTGAYG 250

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LPL+GSTMLRIGMTNPPYILEHLKEIA++L HPCVYSFLHVPV
Sbjct: 251  RDIGVNLPILLNAIVAELPLNGSTMLRIGMTNPPYILEHLKEIADVLCHPCVYSFLHVPV 310

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSD++L+AMNREYTVGEFRKVVDTL ELVPGMQIATDIICGFPGETDEDFA+TV+LIK
Sbjct: 311  QSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDIICGFPGETDEDFAQTVDLIK 370

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYK +QVHISQFYPRPGTPAARMKKV SNVVK+RSRELT+VFESFTPY G+EG +ERIWI
Sbjct: 371  DYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAVFESFTPYTGVEGKVERIWI 430

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TD+A+D +HLVGHTKGY+QVLV+GPES+LGSSA++KITSVGRWSVFG+VIEIL + ND  
Sbjct: 431  TDVASDGVHLVGHTKGYIQVLVIGPESMLGSSAMVKITSVGRWSVFGEVIEILCQ-NDKD 489

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC-KSNSCNAIPETQNNQNAL 304
            +PS    +   ERCSPC+  E+ CACS E+ EPC+  SD C +S +  A    +N+ N  
Sbjct: 490  VPSHWSFD---ERCSPCTLSEEACACSKEQ-EPCSATSDCCNQSPTVEATTLLKNDPNLD 545

Query: 303  NDSS------LLQRRKPSPATVEKEDNSHQKLATQSVRD----GGWALLDTALLGGIFIS 154
            N SS       L+ RK   +  +K D +  +   + ++D      W+L+D ALL GI +S
Sbjct: 546  NHSSRNLIGWFLRNRKNQSS--KKMDLTSSESKEKPIQDLSHWSAWSLVDIALLSGILLS 603

Query: 153  LMTIVAL 133
            L TIVAL
Sbjct: 604  LFTIVAL 610


>ref|XP_009781017.1| PREDICTED: threonylcarbamoyladenosine tRNA
            methylthiotransferase-like, partial [Nicotiana
            sylvestris]
          Length = 594

 Score =  812 bits (2097), Expect = 0.0
 Identities = 414/551 (75%), Positives = 470/551 (85%), Gaps = 13/551 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN + ADLW+INTCTVKSPSQSAMDTLISK +SAKKPLVVAG
Sbjct: 41   SDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSAMDTLISKGRSAKKPLVVAG 100

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL RKTLPALDLPKVRKNKF
Sbjct: 101  CVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKF 160

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 161  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNVIADGVREIWLSSEDTGAYG 220

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LPLDGSTMLRIGMTNPPYILEHLKEIA++LRHPCVY+FLHVPV
Sbjct: 221  RDIGVNLPILLNAIVAELPLDGSTMLRIGMTNPPYILEHLKEIADVLRHPCVYTFLHVPV 280

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSD++L+AMNREYTVGEFRKVVDTL ELVPGMQIATDIICGFPGETDEDF +TV+LIK
Sbjct: 281  QSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDIICGFPGETDEDFGQTVDLIK 340

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYK +QVHISQFYPRPGTPAARMKKV SNVVK+RSR+LT+VFESFTPY GMEG +ERIWI
Sbjct: 341  DYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRKLTAVFESFTPYTGMEGKVERIWI 400

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIA+D IHLVGHTKGY+QVLV+GPES+LG+SA++KITSVGRWSVFG+VIEI+S +N  +
Sbjct: 401  TDIASDGIHLVGHTKGYIQVLVIGPESMLGTSAMVKITSVGRWSVFGEVIEIISHNNK-E 459

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPET-------- 325
            + S EK +   E+CSPC+N E+ CACS E+    +C + +C S S   + ET        
Sbjct: 460  VGSHEKVD---EKCSPCANSEEACACSKEQELGSSCGTTSCCSQS--PMQETTTLLKNEP 514

Query: 324  -QNNQNALNDSSLLQRRKPSPATVEKE----DNSHQKLATQSVRDGGWALLDTALLGGIF 160
             Q++Q + N      R + + ++ + +    ++  +K      R   W  +D ALL GI 
Sbjct: 515  KQDDQGSRNLIGWFLRNRKNHSSKKMDLAGLESKEKKQIQDLGRQSAWGPVDIALLSGIL 574

Query: 159  ISLMTIVALVL 127
            +SL TIVAL L
Sbjct: 575  LSLFTIVALFL 585


>ref|XP_016438122.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase-like
            [Nicotiana tabacum]
          Length = 625

 Score =  813 bits (2100), Expect = 0.0
 Identities = 417/552 (75%), Positives = 466/552 (84%), Gaps = 14/552 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN + ADLW+INTCTVKSPSQSAMDTLISK +SAKKPLVVAG
Sbjct: 72   SDSEYMAGQLSAFGYALSDNPDEADLWMINTCTVKSPSQSAMDTLISKGRSAKKPLVVAG 131

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR+LKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLL RKTLPALDLPKVRKNKF
Sbjct: 132  CVPQGSRNLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKF 191

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 192  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKNVIADGVREIWLSSEDTGAYG 251

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LPLDGSTMLRIGMTNPPYILEHLKEIA++LRHPCVY+FLHVPV
Sbjct: 252  RDIGVNLPILLNAIVAELPLDGSTMLRIGMTNPPYILEHLKEIADVLRHPCVYTFLHVPV 311

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSD++L+AMNREYTVGEFRKVVDTL ELVPGMQIATDIICGFPGETDEDFA+TV+LIK
Sbjct: 312  QSGSDSVLSAMNREYTVGEFRKVVDTLMELVPGMQIATDIICGFPGETDEDFAQTVDLIK 371

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            DYK +QVHISQFYPRPGTPAARMKKV SNVVK+RSRELT+VFESFTPY GMEG +ERIWI
Sbjct: 372  DYKLAQVHISQFYPRPGTPAARMKKVPSNVVKQRSRELTAVFESFTPYTGMEGKVERIWI 431

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIA+D IHLVGHTKGY+QVLV+GPES+LG+SA++KITSVGRWSVFG+VIEI+S++N  +
Sbjct: 432  TDIASDGIHLVGHTKGYIQVLVIGPESMLGASAMVKITSVGRWSVFGEVIEIISQNNK-E 490

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
            + S E+ +   E+CSPC+N E+ CACS E+    +  + +C S S         N+  L+
Sbjct: 491  VGSHERVD---EKCSPCANSEEACACSKEQEPGSSYATTSCCSQSPMQETTLLKNEPKLD 547

Query: 300  DSS-------LLQRRKPSPA-------TVEKEDNSHQKLATQSVRDGGWALLDTALLGGI 163
            D          L+ RK   +          KE    Q L  QS     W  +D ALL G+
Sbjct: 548  DQGSRNLIGWFLRNRKNHSSKKMDLAGLASKEKKQIQDLGRQS----AWGSVDIALLSGV 603

Query: 162  FISLMTIVALVL 127
             +SL TIVAL L
Sbjct: 604  LLSLFTIVALFL 615


>ref|XP_016748500.1| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase-like
            isoform X1 [Gossypium hirsutum]
          Length = 617

 Score =  813 bits (2099), Expect = 0.0
 Identities = 421/562 (74%), Positives = 467/562 (83%), Gaps = 16/562 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVK+PSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 67   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKNPSQSAMNTLITKCKSAKKPLVVAG 126

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLP+LDLP VRKNKF
Sbjct: 127  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPSLDLPNVRKNKF 186

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLV RVK+V+ DGV+EIWLSSEDTGAYG
Sbjct: 187  VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVERVKTVIADGVREIWLSSEDTGAYG 246

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIA +L HPCVYSFLHVPV
Sbjct: 247  RDIGVNLPILLNAIVAELPPDGSTMLRIGMTNPPFILEHLKEIAAVLCHPCVYSFLHVPV 306

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDA+LTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDF++TVNLIK
Sbjct: 307  QSGSDAVLTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQTVNLIK 366

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKFSQVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 367  EYKFSQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYNGMEGKVERIWI 426

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T+IATD IHLVGHTKGYVQVLVV  E++LG+SAI+KITSVGRWSVFG+V+E L + N +K
Sbjct: 427  TEIATDGIHLVGHTKGYVQVLVVAAETILGTSAIVKITSVGRWSVFGEVLESLPQVN-VK 485

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDAC----KSNSCNAIPETQNNQ 313
            +  D+    N E+ SPCSNQ + CACS E PEPC+C  D C    K + C          
Sbjct: 486  MAPDKVS--NQEKHSPCSNQYECCACSKE-PEPCSCGPDICGGQIKLDKCTV-------- 534

Query: 312  NALNDSSLLQRRKPSPA--TVEKEDNSHQK-------LATQSVRD---GGWALLDTALLG 169
             + NDS +  R   +P    + K  N  QK       L  ++ +D   GGW  +D ALL 
Sbjct: 535  -SKNDSPMENRASKNPIHWFIRKRRNHAQKKLENDIALGLENEQDWAPGGWGFVDRALLV 593

Query: 168  GIFISLMTIVALVLYARS*TLI 103
            GIF+S   IVA++++    TL+
Sbjct: 594  GIFVSFFMIVAVIMHLEFRTLL 615


>ref|XP_007020083.2| PREDICTED: threonylcarbamoyladenosine tRNA methylthiotransferase
            [Theobroma cacao]
          Length = 623

 Score =  812 bits (2098), Expect = 0.0
 Identities = 415/556 (74%), Positives = 467/556 (83%), Gaps = 10/556 (1%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVKSPSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 73   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKSPSQSAMNTLITKCKSAKKPLVVAG 132

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL+NRKTLPALDLPKVRKNKF
Sbjct: 133  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLMNRKTLPALDLPKVRKNKF 192

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYT+DSLV RVK+V+ DGVKEIWLSSEDTGAYG
Sbjct: 193  VEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVERVKTVIADGVKEIWLSSEDTGAYG 252

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNA+VA LP DG+TMLRIGMTNPP+ILEHLKEIA++LRHPCVYSFLHVPV
Sbjct: 253  RDIGVNLPILLNALVAELPPDGNTMLRIGMTNPPFILEHLKEIADVLRHPCVYSFLHVPV 312

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDFA+TVNLIK
Sbjct: 313  QSGSDAILTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFAQTVNLIK 372

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKF QVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPY GMEG +ERIWI
Sbjct: 373  EYKFPQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYIGMEGRVERIWI 432

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T++A D IHLVGHTKGYVQVLV+GPES+LG+SAI+KITSVGRWSVFG+VIE L++ N   
Sbjct: 433  TEVAADGIHLVGHTKGYVQVLVIGPESMLGTSAIVKITSVGRWSVFGEVIETLTQVNLKM 492

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
             P   ++ CN E+CS   NQ D+ ACS E PEPC+C  + C   +        +N + + 
Sbjct: 493  AP---EKVCNQEKCSTRFNQFDSGACSKE-PEPCSCGPNCCGGQTILEECTVSSNDSWME 548

Query: 300  DSS-------LLQRRKPSPATVEKEDNSHQKLATQSVRD---GGWALLDTALLGGIFISL 151
            D S       LL++R+     V K+  +   L  +  +D   GGW ++D ALL GI +S 
Sbjct: 549  DRSSKNLIGWLLRKRRNH---VHKKVENGLALGLRKKQDWALGGWGIVDRALLVGILVSF 605

Query: 150  MTIVALVLYARS*TLI 103
              IVA++++    T++
Sbjct: 606  FIIVAVIIHLEFRTIL 621


>gb|PNT07772.1| hypothetical protein POPTR_013G107800v3 [Populus trichocarpa]
          Length = 612

 Score =  811 bits (2095), Expect = 0.0
 Identities = 415/552 (75%), Positives = 463/552 (83%), Gaps = 13/552 (2%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLS+FGY++SD+ E ADLWLINTCTVKSPSQSAMDTLISK KSAKKPLVVAG
Sbjct: 58   SDSEYMAGQLSSFGYSLSDSPEEADLWLINTCTVKSPSQSAMDTLISKGKSAKKPLVVAG 117

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSR++KELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF
Sbjct: 118  CVPQGSRNVKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 177

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYTV+SL GRVK+V++DGVKEIWLSSEDTGAYG
Sbjct: 178  VEILPINVGCLGACTYCKTKHARGHLGSYTVNSLAGRVKTVIDDGVKEIWLSSEDTGAYG 237

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNAIVA LP DGSTMLRIGMTNPP+ILEHLKEIAE+LRHPCVYSFLHVPV
Sbjct: 238  RDIGVNLPILLNAIVAELPSDGSTMLRIGMTNPPFILEHLKEIAEVLRHPCVYSFLHVPV 297

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETD+DF++TVNLIK
Sbjct: 298  QSGSDAILTAMNREYTVNEFRTVVDTLTELVPGMQIATDIICGFPGETDKDFSQTVNLIK 357

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
             YKF+QVHISQFYPRPGTPAARMKKV SN+VK+RSRELTSVFE+FTPYNGMEG +ERIWI
Sbjct: 358  AYKFAQVHISQFYPRPGTPAARMKKVPSNIVKQRSRELTSVFEAFTPYNGMEGRVERIWI 417

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            TDIA D IHLVGHTK YVQVL+V  ES+LG+SAI+KITSVGRWSVFG+VIE L++ N  K
Sbjct: 418  TDIAADGIHLVGHTKAYVQVLIVAQESMLGTSAIVKITSVGRWSVFGEVIETLNQINQ-K 476

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
              S EK   + E+CSPCS+  D+CACS E  EPCAC  ++C   S     +   N+    
Sbjct: 477  SKSVEK-MLSEEKCSPCSDPCDSCACSGES-EPCACGPESCGGQSTIEQSDVLQNEVLRE 534

Query: 300  DSSLLQRRKPSPATVEKEDNSHQKLATQSVRDGG-------------WALLDTALLGGIF 160
            D +   RR      + K  N  QK+    +  G              W ++D ALLGG+ 
Sbjct: 535  DQN---RRNLIGWLLRKRKNQAQKMVENGIASGSQKKQEWAKGAPEEWGVVDRALLGGLI 591

Query: 159  ISLMTIVALVLY 124
            +S+ T VA++++
Sbjct: 592  VSVFTTVAILIH 603


>gb|EOY17308.1| Methylthiotransferase [Theobroma cacao]
          Length = 623

 Score =  811 bits (2095), Expect = 0.0
 Identities = 414/556 (74%), Positives = 467/556 (83%), Gaps = 10/556 (1%)
 Frame = -3

Query: 1740 SDSEYMAGQLSAFGYAVSDNAEGADLWLINTCTVKSPSQSAMDTLISKCKSAKKPLVVAG 1561
            SDSEYMAGQLSAFGYA+SDN E  DLWLINTCTVKSPSQSAM+TLI+KCKSAKKPLVVAG
Sbjct: 73   SDSEYMAGQLSAFGYAISDNPEEGDLWLINTCTVKSPSQSAMNTLITKCKSAKKPLVVAG 132

Query: 1560 CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLLNRKTLPALDLPKVRKNKF 1381
            CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRL+NRKTLP LDLPKVRKNKF
Sbjct: 133  CVPQGSRDLKELEGVSIVGVQQIDRVVEVVEETLKGHEVRLMNRKTLPGLDLPKVRKNKF 192

Query: 1380 VEILPINVGCLGACTYCKTKHARGHLGSYTVDSLVGRVKSVVNDGVKEIWLSSEDTGAYG 1201
            VEILPINVGCLGACTYCKTKHARGHLGSYT+DSLV RVK+V+ DGVKEIWLSSEDTGAYG
Sbjct: 193  VEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVERVKTVIADGVKEIWLSSEDTGAYG 252

Query: 1200 RDIGVNLPILLNAIVAALPLDGSTMLRIGMTNPPYILEHLKEIAEILRHPCVYSFLHVPV 1021
            RDIGVNLPILLNA+VA LP DG+TMLRIGMTNPP+ILEHLKEIA++LRHPCVYSFLHVPV
Sbjct: 253  RDIGVNLPILLNALVAELPPDGNTMLRIGMTNPPFILEHLKEIADVLRHPCVYSFLHVPV 312

Query: 1020 QSGSDAILTAMNREYTVGEFRKVVDTLTELVPGMQIATDIICGFPGETDEDFAETVNLIK 841
            QSGSDAILTAMNREYTV EFR VVDTLTELVPGMQIATDIICGFPGETDEDFA+TVNLIK
Sbjct: 313  QSGSDAILTAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFAQTVNLIK 372

Query: 840  DYKFSQVHISQFYPRPGTPAARMKKVASNVVKKRSRELTSVFESFTPYNGMEGNMERIWI 661
            +YKF QVHISQFYPRPGTPAARMKKV S +VK RSRELTSVFE+FTPY GMEG +ERIWI
Sbjct: 373  EYKFPQVHISQFYPRPGTPAARMKKVPSTIVKNRSRELTSVFEAFTPYIGMEGRVERIWI 432

Query: 660  TDIATDEIHLVGHTKGYVQVLVVGPESLLGSSAIIKITSVGRWSVFGDVIEILSKSNDLK 481
            T++A D IHLVGHTKGYVQVLV+GPES+LG+SAI+KITSVGRWSVFG+VIE L++ N   
Sbjct: 433  TEVAADGIHLVGHTKGYVQVLVIGPESMLGTSAIVKITSVGRWSVFGEVIETLTQVNLKM 492

Query: 480  IPSDEKENCNTERCSPCSNQEDTCACSSEKPEPCACESDACKSNSCNAIPETQNNQNALN 301
             P   ++ CN E+CS   NQ D+ ACS E PEPC+C  ++C   +        +N + + 
Sbjct: 493  AP---EKVCNQEKCSTRFNQFDSGACSKE-PEPCSCGPNSCGGQTILEECTVSSNDSWME 548

Query: 300  DSS-------LLQRRKPSPATVEKEDNSHQKLATQSVRD---GGWALLDTALLGGIFISL 151
            D S       LL++R+     V K+  +   L  +  +D   GGW ++D ALL GI +S 
Sbjct: 549  DRSSKNLIGWLLRKRRNH---VHKKVENGLALGLRKKQDWALGGWGIVDRALLVGILVSF 605

Query: 150  MTIVALVLYARS*TLI 103
              IVA++++    T++
Sbjct: 606  FIIVAVIIHLEFRTML 621