BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00013259
         (2026 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVH94413.1| Glycoside hydrolase, catalytic domain-containing ...  1127   0.0  
ref|XP_022010633.1| isoamylase 2, chloroplastic [Helianthus annu...  1109   0.0  
gb|OTF93917.1| putative debranching enzyme 1 [Helianthus annuus]     1109   0.0  
ref|XP_023738402.1| isoamylase 2, chloroplastic isoform X1 [Lact...  1051   0.0  
ref|XP_023738403.1| isoamylase 2, chloroplastic isoform X2 [Lact...  1051   0.0  
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             898   0.0  
gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus cap...   881   0.0  
ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]        876   0.0  
gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olito...   875   0.0  
ref|XP_022771074.1| isoamylase 2, chloroplastic-like [Durio zibe...   874   0.0  
gb|KZN10097.1| hypothetical protein DCAR_002753 [Daucus carota s...   868   0.0  
ref|XP_017978673.1| PREDICTED: isoamylase 2, chloroplastic [Theo...   871   0.0  
ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prun...   871   0.0  
gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]                 870   0.0  
ref|XP_017230565.1| PREDICTED: isoamylase 2, chloroplastic [Dauc...   868   0.0  
ref|XP_022758172.1| isoamylase 2, chloroplastic-like [Durio zibe...   868   0.0  
ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]...   867   0.0  
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic [Frag...   865   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   866   0.0  
ref|XP_021281244.1| isoamylase 2, chloroplastic [Herrania umbrat...   865   0.0  

>gb|KVH94413.1| Glycoside hydrolase, catalytic domain-containing protein [Cynara
            cardunculus var. scolymus]
          Length = 841

 Score = 1127 bits (2915), Expect = 0.0
 Identities = 545/680 (80%), Positives = 600/680 (88%), Gaps = 8/680 (1%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSYS 1846
            NKLSVELEFDASLAPFY+SFLLKS L+GEKIEI SHR T FCFPVG++SGYPAPLG+SY+
Sbjct: 163  NKLSVELEFDASLAPFYISFLLKSQLSGEKIEIRSHRNTNFCFPVGIRSGYPAPLGLSYT 222

Query: 1845 ADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSY 1666
            ADG INFALFSRNAK+V+LCLFDDSSKEEPALEIDLDPY+NRSGDIWHAS+ S MNFVSY
Sbjct: 223  ADGFINFALFSRNAKSVVLCLFDDSSKEEPALEIDLDPYINRSGDIWHASMDSAMNFVSY 282

Query: 1665 GYRCRNDAQNKSPFSSVLLDPYSKVIGENFLGEICKEPTFDWSGDIRPNVPMEKLMVYRL 1486
            GYRCRN AQ+KS    V LDPY+KVIGENFLG+IC EP FDWSGD+ P + MEKLMVYRL
Sbjct: 283  GYRCRNGAQDKSQVKRVSLDPYAKVIGENFLGKICTEPAFDWSGDVHPRLSMEKLMVYRL 342

Query: 1485 NVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKGPYFPSHFF 1306
            NVMDFT+D SSNLP+  GGTF GV+EKLHHFKDLGV+AILLEPIVP+DKQ GPYFP HFF
Sbjct: 343  NVMDFTKDTSSNLPNEIGGTFLGVSEKLHHFKDLGVNAILLEPIVPFDKQVGPYFPFHFF 402

Query: 1305 SPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKIDKPSYY--- 1135
            S  +S+GPPGGS+ TI SMKEMVKRLH++GIE+LLEVV +HT ++ASLTKID  SYY   
Sbjct: 403  SLHDSFGPPGGSMPTIKSMKEMVKRLHADGIEILLEVVLTHTSQNASLTKIDNSSYYFVK 462

Query: 1134 -----SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGENLSRPPL 970
                 + K TLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM+RGF GENLSRPPL
Sbjct: 463  EGDDLATKCTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMLRGFNGENLSRPPL 522

Query: 969  IEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYLRGESLLS 790
            IEAIAFDPILSKTK+IADSFDP HKS KEIQFPHWKRWAEMNT FC DARNYLRGESLLS
Sbjct: 523  IEAIAFDPILSKTKLIADSFDPFHKSAKEIQFPHWKRWAEMNTNFCKDARNYLRGESLLS 582

Query: 789  DLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKTTELSWNCGEEGATKNKS 610
            +LATRLCGSGDIFL+GRGPAFSFNF+T NHGFSLVDLVSF+ T ELSWNCGEEGATK KS
Sbjct: 583  NLATRLCGSGDIFLNGRGPAFSFNFVTRNHGFSLVDLVSFNNTRELSWNCGEEGATKKKS 642

Query: 609  VLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFNWKALQTGFAT 430
            VLETRLKQIRNF+FILYISLGVPV+ MGDECG+SSG  G L+  DRKPFNWKALQTGF  
Sbjct: 643  VLETRLKQIRNFIFILYISLGVPVLTMGDECGRSSG--GLLEQGDRKPFNWKALQTGFGI 700

Query: 429  QTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKFLAMSLKVDQA 250
            QTTQF+SFLSALKIRRSDL QS EFLKVENIDW+GPNL+PPDWEDPTS+FLAM LKVD+ 
Sbjct: 701  QTTQFVSFLSALKIRRSDLLQSREFLKVENIDWHGPNLSPPDWEDPTSRFLAMRLKVDED 760

Query: 249  DKKLLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYPKFFSVTGEPVVEKIPG 70
            D+  L GDVFVA NGGDEPV+ +VP P S M WVRLVDTAL YP+FFS  G+ V EKIPG
Sbjct: 761  DENQLKGDVFVAINGGDEPVMAAVPLPGSKMAWVRLVDTALQYPRFFSTAGDRVPEKIPG 820

Query: 69   SLTYELVPHSCVLLESKSVD 10
            S  Y++ PHSCVLLE++S+D
Sbjct: 821  SPVYDMGPHSCVLLEARSID 840


>ref|XP_022010633.1| isoamylase 2, chloroplastic [Helianthus annuus]
 ref|XP_022010634.1| isoamylase 2, chloroplastic [Helianthus annuus]
          Length = 831

 Score = 1109 bits (2869), Expect = 0.0
 Identities = 530/673 (78%), Positives = 591/673 (87%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSYS 1846
            NK S ELEFDASLAPFY+SFLLK  L+GE +EI SHRKT FCFPVG+KSG P PLGVSYS
Sbjct: 164  NKFSAELEFDASLAPFYISFLLKWHLDGETVEIKSHRKTNFCFPVGVKSGRPGPLGVSYS 223

Query: 1845 ADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSY 1666
            ADGS+NFALFSRNAK+VILCLFDDSS EEP +EIDLDPYVNRSGDIWH S+ + MNFVSY
Sbjct: 224  ADGSLNFALFSRNAKSVILCLFDDSSSEEPVIEIDLDPYVNRSGDIWHVSMDNAMNFVSY 283

Query: 1665 GYRCRNDAQNKSPFSSVLLDPYSKVIGENFLGEICKEPTFDWSGDIRPNVPMEKLMVYRL 1486
            GYR +ND Q KS   SV+LDPYSKVI EN LG+ICKEPTFDWS D+RPN+PMEKL VYRL
Sbjct: 284  GYRVKNDGQEKS---SVVLDPYSKVIAENSLGKICKEPTFDWSDDVRPNLPMEKLTVYRL 340

Query: 1485 NVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKGPYFPSHFF 1306
            NVMDFTRDASSNLPD   GTF+ ++EKLHH KDLGV+A+LLEPI PYDK KGPY+PSHFF
Sbjct: 341  NVMDFTRDASSNLPDEIRGTFSAISEKLHHLKDLGVNAVLLEPIAPYDKLKGPYYPSHFF 400

Query: 1305 SPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKIDKPSYYSDK 1126
            SPQNSYGP G S STINSM EMVKRLH+NGIEVLLEVV +HTC+ +SL KIDKPSYY   
Sbjct: 401  SPQNSYGPHGDSTSTINSMNEMVKRLHANGIEVLLEVVVTHTCDSSSLAKIDKPSYYYSN 460

Query: 1125 NTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGENLSRPPLIEAIAFDP 946
            N LNCAHPVVQQLILDSL+HWVI YHIDGFCFI++SSMI GF GE+LSRPPLIEAIAFDP
Sbjct: 461  NALNCAHPVVQQLILDSLKHWVINYHIDGFCFIDSSSMINGFNGEHLSRPPLIEAIAFDP 520

Query: 945  ILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYLRGESLLSDLATRLCG 766
            +LSKTKIIADSFDP+  S KEIQFPHWK+WAEMN++FCSD RNYLRGESL+S+LATRLCG
Sbjct: 521  VLSKTKIIADSFDPLLNSTKEIQFPHWKKWAEMNSRFCSDVRNYLRGESLVSNLATRLCG 580

Query: 765  SGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKTTELSWNCGEEGATKNKSVLETRLKQ 586
            SGDIFL+GRGPAFSFNF+  NHG SLVDLVSFS  +ELSWNCGEEGATK ++VLETRLKQ
Sbjct: 581  SGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSSASELSWNCGEEGATKKRTVLETRLKQ 640

Query: 585  IRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFNWKALQTGFATQTTQFISF 406
            IRNFLFILY+SLGVPV+NMGDECGQSSGGK  +   +RKPFNWK+L+TGFA QT +FISF
Sbjct: 641  IRNFLFILYVSLGVPVLNMGDECGQSSGGK--ISVKERKPFNWKSLKTGFAVQTIEFISF 698

Query: 405  LSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKFLAMSLKVDQADKKLLTGD 226
            LS+LKI+RSDL Q  EFLKVENIDWYGP+L+ P+WEDPTSKFLAMSLK DQ D+  LT D
Sbjct: 699  LSSLKIKRSDLLQRREFLKVENIDWYGPDLSLPNWEDPTSKFLAMSLKCDQNDENALTSD 758

Query: 225  VFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYPKFFSVTGEPVVEKIPGSLTYELVP 46
            +FVAFNGGD PV  ++PPPR  M WVRLVDT LPYP FFS TGEPVVEKIPGSL Y++ P
Sbjct: 759  MFVAFNGGDSPVTATLPPPRLGMEWVRLVDTGLPYPGFFSTTGEPVVEKIPGSLGYQMTP 818

Query: 45   HSCVLLESKSVDG 7
            HSCVLLE+++VDG
Sbjct: 819  HSCVLLEARNVDG 831


>gb|OTF93917.1| putative debranching enzyme 1 [Helianthus annuus]
          Length = 847

 Score = 1109 bits (2869), Expect = 0.0
 Identities = 530/673 (78%), Positives = 591/673 (87%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSYS 1846
            NK S ELEFDASLAPFY+SFLLK  L+GE +EI SHRKT FCFPVG+KSG P PLGVSYS
Sbjct: 180  NKFSAELEFDASLAPFYISFLLKWHLDGETVEIKSHRKTNFCFPVGVKSGRPGPLGVSYS 239

Query: 1845 ADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSY 1666
            ADGS+NFALFSRNAK+VILCLFDDSS EEP +EIDLDPYVNRSGDIWH S+ + MNFVSY
Sbjct: 240  ADGSLNFALFSRNAKSVILCLFDDSSSEEPVIEIDLDPYVNRSGDIWHVSMDNAMNFVSY 299

Query: 1665 GYRCRNDAQNKSPFSSVLLDPYSKVIGENFLGEICKEPTFDWSGDIRPNVPMEKLMVYRL 1486
            GYR +ND Q KS   SV+LDPYSKVI EN LG+ICKEPTFDWS D+RPN+PMEKL VYRL
Sbjct: 300  GYRVKNDGQEKS---SVVLDPYSKVIAENSLGKICKEPTFDWSDDVRPNLPMEKLTVYRL 356

Query: 1485 NVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKGPYFPSHFF 1306
            NVMDFTRDASSNLPD   GTF+ ++EKLHH KDLGV+A+LLEPI PYDK KGPY+PSHFF
Sbjct: 357  NVMDFTRDASSNLPDEIRGTFSAISEKLHHLKDLGVNAVLLEPIAPYDKLKGPYYPSHFF 416

Query: 1305 SPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKIDKPSYYSDK 1126
            SPQNSYGP G S STINSM EMVKRLH+NGIEVLLEVV +HTC+ +SL KIDKPSYY   
Sbjct: 417  SPQNSYGPHGDSTSTINSMNEMVKRLHANGIEVLLEVVVTHTCDSSSLAKIDKPSYYYSN 476

Query: 1125 NTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGENLSRPPLIEAIAFDP 946
            N LNCAHPVVQQLILDSL+HWVI YHIDGFCFI++SSMI GF GE+LSRPPLIEAIAFDP
Sbjct: 477  NALNCAHPVVQQLILDSLKHWVINYHIDGFCFIDSSSMINGFNGEHLSRPPLIEAIAFDP 536

Query: 945  ILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYLRGESLLSDLATRLCG 766
            +LSKTKIIADSFDP+  S KEIQFPHWK+WAEMN++FCSD RNYLRGESL+S+LATRLCG
Sbjct: 537  VLSKTKIIADSFDPLLNSTKEIQFPHWKKWAEMNSRFCSDVRNYLRGESLVSNLATRLCG 596

Query: 765  SGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKTTELSWNCGEEGATKNKSVLETRLKQ 586
            SGDIFL+GRGPAFSFNF+  NHG SLVDLVSFS  +ELSWNCGEEGATK ++VLETRLKQ
Sbjct: 597  SGDIFLNGRGPAFSFNFVARNHGLSLVDLVSFSSASELSWNCGEEGATKKRTVLETRLKQ 656

Query: 585  IRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFNWKALQTGFATQTTQFISF 406
            IRNFLFILY+SLGVPV+NMGDECGQSSGGK  +   +RKPFNWK+L+TGFA QT +FISF
Sbjct: 657  IRNFLFILYVSLGVPVLNMGDECGQSSGGK--ISVKERKPFNWKSLKTGFAVQTIEFISF 714

Query: 405  LSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKFLAMSLKVDQADKKLLTGD 226
            LS+LKI+RSDL Q  EFLKVENIDWYGP+L+ P+WEDPTSKFLAMSLK DQ D+  LT D
Sbjct: 715  LSSLKIKRSDLLQRREFLKVENIDWYGPDLSLPNWEDPTSKFLAMSLKCDQNDENALTSD 774

Query: 225  VFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYPKFFSVTGEPVVEKIPGSLTYELVP 46
            +FVAFNGGD PV  ++PPPR  M WVRLVDT LPYP FFS TGEPVVEKIPGSL Y++ P
Sbjct: 775  MFVAFNGGDSPVTATLPPPRLGMEWVRLVDTGLPYPGFFSTTGEPVVEKIPGSLGYQMTP 834

Query: 45   HSCVLLESKSVDG 7
            HSCVLLE+++VDG
Sbjct: 835  HSCVLLEARNVDG 847


>ref|XP_023738402.1| isoamylase 2, chloroplastic isoform X1 [Lactuca sativa]
          Length = 876

 Score = 1051 bits (2718), Expect = 0.0
 Identities = 517/679 (76%), Positives = 582/679 (85%), Gaps = 6/679 (0%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSYS 1846
            NKLS ELEFDASLAPF+VSFLLKS   GEKIEI SHR+T FCFPVG++SG PAPLG+SYS
Sbjct: 208  NKLSTELEFDASLAPFHVSFLLKSHFGGEKIEIRSHRRTNFCFPVGIRSGSPAPLGLSYS 267

Query: 1845 ADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSY 1666
            ADGSINFALFSRNAK VILCLF++ SKE PALE DLDPYVNRSGDIWHAS+ SDMNFVSY
Sbjct: 268  ADGSINFALFSRNAKGVILCLFENKSKENPALEFDLDPYVNRSGDIWHASMDSDMNFVSY 327

Query: 1665 GYRCRNDAQNKSPFSSVLLDPYSKVIGENFLGEICKEPTFDWSGDIRPNVPMEKLMVYRL 1486
            GYR RN  Q+KS    VLLDPY+KVIGE F+GEICK+PTFDWS +IRP +PMEKLMVYRL
Sbjct: 328  GYRIRNSGQDKSYEHQVLLDPYAKVIGEKFIGEICKQPTFDWSDEIRPCLPMEKLMVYRL 387

Query: 1485 NVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKGPYFPSHFF 1306
            NV DFT+D SSNL    GGTF GV+EKL HF++LGV+AILLEPIVPYDKQ GPYFPSHFF
Sbjct: 388  NVTDFTKDPSSNLSKEVGGTFLGVSEKLDHFRNLGVNAILLEPIVPYDKQVGPYFPSHFF 447

Query: 1305 SPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKIDKPSYY-SD 1129
            SP+N          TI S+KEMVKRLH+NGIEVL+EVV +HT + ASLTKID+PSYY S 
Sbjct: 448  SPRNPL--------TIQSLKEMVKRLHANGIEVLMEVVLTHTSQTASLTKIDRPSYYLST 499

Query: 1128 KNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGENLSRPPLIEAIAFD 949
            KN LNCAHPVVQQLILDSLR+WVIEYHIDGFCFINASSM+RGF GE LSRPPLIEAI+FD
Sbjct: 500  KNELNCAHPVVQQLILDSLRNWVIEYHIDGFCFINASSMLRGFNGEILSRPPLIEAISFD 559

Query: 948  PILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYLRGESLLSDLATRLC 769
            PILS TK+IADSFDP+ KS KEIQFPHWKRWAEMNT+F  D RNYLRGE+L+S+LATR+C
Sbjct: 560  PILSNTKLIADSFDPVQKSTKEIQFPHWKRWAEMNTEFFRDTRNYLRGETLVSNLATRIC 619

Query: 768  GSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKTTELSWNCGEEGATKNKSVLETRLK 589
            GSGDIFL+GRGPAF FNF++ NHG +LVD VSFS T+ELSWNCGEEGATK KSVLETRLK
Sbjct: 620  GSGDIFLNGRGPAFCFNFVSRNHGLTLVDSVSFSNTSELSWNCGEEGATKRKSVLETRLK 679

Query: 588  QIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFNWKALQTGFATQTTQFIS 409
            QIRN+LFILYISLGVPV+NMGDECGQSSGG   +  SDRKPFNW ALQTGFA QTT+FIS
Sbjct: 680  QIRNYLFILYISLGVPVLNMGDECGQSSGGS--VNRSDRKPFNWNALQTGFAIQTTEFIS 737

Query: 408  FLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKFLAMSLKVDQA----DKK 241
            FLSALKIRRSDL Q  EFLKVENIDWYG NL+ P+WEDPTSKFLA+SLKV++     D+ 
Sbjct: 738  FLSALKIRRSDLLQRREFLKVENIDWYGTNLSLPNWEDPTSKFLAVSLKVEKEINRDDEN 797

Query: 240  LLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYPKFFSVTGEPVVEKIPGSLT 61
            +L GD+F AFNGGDE V  +VPPP +DM WVRLVDT+LP+P FFSVTG+ + E +PG+  
Sbjct: 798  MLDGDMFAAFNGGDEAVTATVPPPPADMAWVRLVDTSLPFPGFFSVTGDSIPEMMPGAPA 857

Query: 60   YELVPHSCVLLES-KSVDG 7
            YE+  HSCVLLE+ + +DG
Sbjct: 858  YEVASHSCVLLEARRRIDG 876


>ref|XP_023738403.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 ref|XP_023738404.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 ref|XP_023738405.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 ref|XP_023738406.1| isoamylase 2, chloroplastic isoform X2 [Lactuca sativa]
 gb|PLY70241.1| hypothetical protein LSAT_9X4440 [Lactuca sativa]
          Length = 827

 Score = 1051 bits (2718), Expect = 0.0
 Identities = 517/679 (76%), Positives = 582/679 (85%), Gaps = 6/679 (0%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSYS 1846
            NKLS ELEFDASLAPF+VSFLLKS   GEKIEI SHR+T FCFPVG++SG PAPLG+SYS
Sbjct: 159  NKLSTELEFDASLAPFHVSFLLKSHFGGEKIEIRSHRRTNFCFPVGIRSGSPAPLGLSYS 218

Query: 1845 ADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSY 1666
            ADGSINFALFSRNAK VILCLF++ SKE PALE DLDPYVNRSGDIWHAS+ SDMNFVSY
Sbjct: 219  ADGSINFALFSRNAKGVILCLFENKSKENPALEFDLDPYVNRSGDIWHASMDSDMNFVSY 278

Query: 1665 GYRCRNDAQNKSPFSSVLLDPYSKVIGENFLGEICKEPTFDWSGDIRPNVPMEKLMVYRL 1486
            GYR RN  Q+KS    VLLDPY+KVIGE F+GEICK+PTFDWS +IRP +PMEKLMVYRL
Sbjct: 279  GYRIRNSGQDKSYEHQVLLDPYAKVIGEKFIGEICKQPTFDWSDEIRPCLPMEKLMVYRL 338

Query: 1485 NVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKGPYFPSHFF 1306
            NV DFT+D SSNL    GGTF GV+EKL HF++LGV+AILLEPIVPYDKQ GPYFPSHFF
Sbjct: 339  NVTDFTKDPSSNLSKEVGGTFLGVSEKLDHFRNLGVNAILLEPIVPYDKQVGPYFPSHFF 398

Query: 1305 SPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKIDKPSYY-SD 1129
            SP+N          TI S+KEMVKRLH+NGIEVL+EVV +HT + ASLTKID+PSYY S 
Sbjct: 399  SPRNPL--------TIQSLKEMVKRLHANGIEVLMEVVLTHTSQTASLTKIDRPSYYLST 450

Query: 1128 KNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGENLSRPPLIEAIAFD 949
            KN LNCAHPVVQQLILDSLR+WVIEYHIDGFCFINASSM+RGF GE LSRPPLIEAI+FD
Sbjct: 451  KNELNCAHPVVQQLILDSLRNWVIEYHIDGFCFINASSMLRGFNGEILSRPPLIEAISFD 510

Query: 948  PILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYLRGESLLSDLATRLC 769
            PILS TK+IADSFDP+ KS KEIQFPHWKRWAEMNT+F  D RNYLRGE+L+S+LATR+C
Sbjct: 511  PILSNTKLIADSFDPVQKSTKEIQFPHWKRWAEMNTEFFRDTRNYLRGETLVSNLATRIC 570

Query: 768  GSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKTTELSWNCGEEGATKNKSVLETRLK 589
            GSGDIFL+GRGPAF FNF++ NHG +LVD VSFS T+ELSWNCGEEGATK KSVLETRLK
Sbjct: 571  GSGDIFLNGRGPAFCFNFVSRNHGLTLVDSVSFSNTSELSWNCGEEGATKRKSVLETRLK 630

Query: 588  QIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFNWKALQTGFATQTTQFIS 409
            QIRN+LFILYISLGVPV+NMGDECGQSSGG   +  SDRKPFNW ALQTGFA QTT+FIS
Sbjct: 631  QIRNYLFILYISLGVPVLNMGDECGQSSGGS--VNRSDRKPFNWNALQTGFAIQTTEFIS 688

Query: 408  FLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKFLAMSLKVDQA----DKK 241
            FLSALKIRRSDL Q  EFLKVENIDWYG NL+ P+WEDPTSKFLA+SLKV++     D+ 
Sbjct: 689  FLSALKIRRSDLLQRREFLKVENIDWYGTNLSLPNWEDPTSKFLAVSLKVEKEINRDDEN 748

Query: 240  LLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYPKFFSVTGEPVVEKIPGSLT 61
            +L GD+F AFNGGDE V  +VPPP +DM WVRLVDT+LP+P FFSVTG+ + E +PG+  
Sbjct: 749  MLDGDMFAAFNGGDEAVTATVPPPPADMAWVRLVDTSLPFPGFFSVTGDSIPEMMPGAPA 808

Query: 60   YELVPHSCVLLES-KSVDG 7
            YE+  HSCVLLE+ + +DG
Sbjct: 809  YEVASHSCVLLEARRRIDG 827


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  898 bits (2320), Expect = 0.0
 Identities = 440/698 (63%), Positives = 536/698 (76%), Gaps = 28/698 (4%)
 Frame = -2

Query: 2022 KLSVELEFDASLAPFYVSFLLKSSLNGEK--IEIGSHRKTKFCFPVGLKSGYPAPLGVSY 1849
            KL +EL F+ SL PFY+SFLLK   +G+    ++ SHRKT FC PVGL SGYPAPLG+S+
Sbjct: 169  KLVIELNFEGSLTPFYLSFLLKFPSDGDSKSSDLRSHRKTSFCVPVGLASGYPAPLGLSF 228

Query: 1848 SADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVS 1669
            SADGSINFALFS++A  V+LCL+DD+  E+P LEIDLDPYVNRSGDIWH S+ S + FVS
Sbjct: 229  SADGSINFALFSQSADGVVLCLYDDTKAEKPTLEIDLDPYVNRSGDIWHVSMDSALPFVS 288

Query: 1668 YGYRCRNDAQNKSP---FSSVLLDPYSKVIGE-------NFLGEICKEPTFDWSGDIRPN 1519
            YGYRC+     K        VLLDPY+KVI           LG++ KEPTFDWSGDIRP 
Sbjct: 289  YGYRCKGGIHGKGVKDHSEHVLLDPYAKVIANAHSGSVLKTLGKLIKEPTFDWSGDIRPC 348

Query: 1518 VPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDK 1339
            +PMEKL+VYR+NV  FT+  SS LP+   G+FTG+ EKL H KDLGV+AILLEP+ P+D+
Sbjct: 349  IPMEKLVVYRVNVAHFTKHKSSGLPNDVAGSFTGITEKLQHCKDLGVNAILLEPVFPFDE 408

Query: 1338 QKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLT 1159
            +KGPYFP HFFSP   YGP     +T+NSMKEMVK LH+NGIEVLLE+VF+HT E  SL 
Sbjct: 409  KKGPYFPFHFFSPMKLYGPSDDPSATVNSMKEMVKILHANGIEVLLEIVFTHTAESCSLR 468

Query: 1158 KIDKPSYY-------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGF 1000
            +IDK SYY         KN LNC +P+VQQ+ILDSLR+W IE+H+DGFCFINASS+++GF
Sbjct: 469  EIDKSSYYITGDEDLGAKNALNCNYPIVQQMILDSLRYWAIEFHVDGFCFINASSLLKGF 528

Query: 999  KGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDAR 820
             GE LSRPPL+EAIAFDP+LSKTK+IADS+DP   +  EI FPHWKRWAE+NT FC D R
Sbjct: 529  HGEFLSRPPLVEAIAFDPLLSKTKLIADSWDPHDMASDEILFPHWKRWAEVNTNFCGDVR 588

Query: 819  NYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKT---TELS 649
            N+LRGE LLS+LATRLCGSGD+FL+GRGP FSFN+IT N G  LVDLVSFS +   +ELS
Sbjct: 589  NFLRGEGLLSNLATRLCGSGDMFLNGRGPGFSFNYITRNSGLPLVDLVSFSSSNLASELS 648

Query: 648  WNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRK 469
            WNCGEEG T N +VLE RLKQIRN+LFIL++S GVPV+NMGDECGQSSGG     Y DRK
Sbjct: 649  WNCGEEGPTNNTAVLERRLKQIRNYLFILFVSFGVPVLNMGDECGQSSGGSP--AYGDRK 706

Query: 468  PFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPT 289
            P++W AL+TGF  QTTQFISFLS+L++RRSDL Q   FLK ENIDWYG + + P W+D +
Sbjct: 707  PYDWNALRTGFGIQTTQFISFLSSLRVRRSDLLQKRSFLKEENIDWYGSDQSLPTWDDRS 766

Query: 288  SKFLAMSLKVDQADKK------LLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTAL 127
            S+FLAM+LKV++ + +       L GD+FVAFN  D+     +PPP  +MVW+RLVDTA+
Sbjct: 767  SRFLAMTLKVEREENESSSEFSSLNGDLFVAFNSADQSERVILPPPPPEMVWLRLVDTAV 826

Query: 126  PYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSV 13
            P+P FFS  GEPV+E++ G +TYE+  HSCVL E+K +
Sbjct: 827  PFPGFFSRDGEPVLEQMAGLVTYEMKSHSCVLFEAKKL 864


>gb|OMO77794.1| hypothetical protein CCACVL1_14832 [Corchorus capsularis]
          Length = 878

 Score =  881 bits (2276), Expect = 0.0
 Identities = 434/703 (61%), Positives = 524/703 (74%), Gaps = 31/703 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSS--LNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++ELEF+A  APFY SFLLK+S   N   +EI SHRKT FC P+G   GYP PLG+S
Sbjct: 168  SELALELEFEAKEAPFYFSFLLKASSDANSSGLEIRSHRKTNFCVPIGFDQGYPVPLGLS 227

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S DGS+NFA++SRNA++++LCL+DD+  E+PALE+DLDPYVNR+GDIWHASL     FV
Sbjct: 228  FSNDGSMNFAVYSRNAESLVLCLYDDTGSEKPALELDLDPYVNRTGDIWHASLEGAWTFV 287

Query: 1671 SYGYRCRNDAQNKSPFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSGDI 1528
            SYGYRC+ D  +      VLLDPY+K+IG +             LG +CKEP FDWSGD+
Sbjct: 288  SYGYRCKGDG-DAFHAERVLLDPYAKIIGSSIPNHYESGLLLKHLGRLCKEPAFDWSGDV 346

Query: 1527 RPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVP 1348
             PN+P+EKL+VYRLNVM FT D SS LP    GTF+GV EK+ H K LG++A+LLEPI  
Sbjct: 347  CPNLPLEKLVVYRLNVMHFTEDKSSKLPANVAGTFSGVTEKVQHLKYLGINAVLLEPIFT 406

Query: 1347 YDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDA 1168
            +D+QKGPYFP HFFSP + YGP  GSIS INS+K MVK LH+NGIEVLLEVVF+HT E  
Sbjct: 407  FDEQKGPYFPCHFFSPTSLYGPSNGSISAINSIKGMVKNLHANGIEVLLEVVFTHTAEGG 466

Query: 1167 SLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM 1012
            +L  +D  SYY         +KN LNC +PVVQQ+ILDSLRHWV E+HIDGFCFINAS +
Sbjct: 467  ALQGLDDESYYYRNRVEDLEEKNALNCNYPVVQQMILDSLRHWVTEFHIDGFCFINASCL 526

Query: 1011 IRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFC 832
            +RGF GE+LSRPPL+EAIAFDP+LSKTK+IAD +DP     KEI FPHWKRWAEMNTKFC
Sbjct: 527  LRGFHGEHLSRPPLVEAIAFDPLLSKTKVIADCWDPHEMMPKEIHFPHWKRWAEMNTKFC 586

Query: 831  SDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK---T 661
            SD RN+LRGE  LS LATRLCGSGDIF  GRGPAFSFNFI  N G  LVDLVSFS     
Sbjct: 587  SDIRNFLRGEDALSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSNAELA 646

Query: 660  TELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKY 481
            +ELSWNCGEEG T N +VLE RLKQIRN++FIL++SLGVPV+NMGDECGQSSGG   L Y
Sbjct: 647  SELSWNCGEEGPTSNTAVLERRLKQIRNYIFILFVSLGVPVLNMGDECGQSSGGS--LSY 704

Query: 480  SDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDW 301
              RK  +W A+ TGF  QTTQFISFLS+L++RRSDL Q   FLK ENI+W+G + +PP+W
Sbjct: 705  GSRKLLDWNAMTTGFGIQTTQFISFLSSLRVRRSDLLQRRNFLKEENIEWHGSSQSPPEW 764

Query: 300  EDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            EDP+ KFLAM+LK D+A+  L      L GD+F+A N  D+     +PP    M W RLV
Sbjct: 765  EDPSCKFLAMTLKADKAESLLSSEASELKGDLFIAINADDKTENIILPPCPEGMAWRRLV 824

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSVD 10
            DTALPYP FF   G+ V+E++ G + YE+   SC L E+ + D
Sbjct: 825  DTALPYPGFFLADGKAVLEQMAGLVAYEMKSRSCTLFEACTED 867


>ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]
          Length = 883

 Score =  876 bits (2263), Expect = 0.0
 Identities = 435/704 (61%), Positives = 526/704 (74%), Gaps = 34/704 (4%)
 Frame = -2

Query: 2022 KLSVELEFDASLAPFYVSFLLKS--SLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSY 1849
            + + ELEF+A   PFY SF+L S    N   +EI SHRKT FC PVG   GYP PLG+++
Sbjct: 182  RFTFELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTF 241

Query: 1848 SADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVS 1669
            S DGS+NFA+FSRNA++V+LCL+DD++ E+PALE+DLDPYVNRSGDIWH S GS   FVS
Sbjct: 242  SNDGSMNFAIFSRNAESVVLCLYDDTTAEKPALELDLDPYVNRSGDIWHVSFGSARAFVS 301

Query: 1668 YGYRCRNDA--QNKSPFSS--VLLDPYSKVI----------GENFLGEICKEPTFDWSGD 1531
            YGY+ + +    NK+ F    VLLDPY+KVI          G  +LG +C+EP FDW+GD
Sbjct: 302  YGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAGD 361

Query: 1530 IRPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIV 1351
            +RP++ MEKL+VYRLNVM FT   SS LP   GG+F+G+ EKL HFKDLGV+A+LLEPI 
Sbjct: 362  VRPDLSMEKLVVYRLNVMRFTEHKSSQLPTDIGGSFSGLTEKLEHFKDLGVNAVLLEPIF 421

Query: 1350 PYDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCED 1171
            P+D+QKGPYFP HFFSP + +GP GG +S +NSMKEMV++ H+NGIEVLLEVVF+HT E 
Sbjct: 422  PFDEQKGPYFPRHFFSPMDCFGPSGGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAEG 481

Query: 1170 ASLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASS 1015
             +L  ID  SYY          +N LNC +PVVQQL+LDSLR+WV E+H+DGFCFINASS
Sbjct: 482  EALQGIDISSYYHVNEVEDLEARNALNCNYPVVQQLVLDSLRYWVTEFHVDGFCFINASS 541

Query: 1014 MIRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKF 835
            ++RGF GE LSRPPL+EAIAFDP+LSKTKIIAD +DP   + KE +FPHWKRWAE+NTKF
Sbjct: 542  LLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADRWDPHGMAPKETRFPHWKRWAEVNTKF 601

Query: 834  CSDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFS---K 664
            C+D RN+LRGE LLSDLATRLCG+GDIF  GRGPAF+FNFI+ N G  LVDLVSFS    
Sbjct: 602  CNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVEL 661

Query: 663  TTELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLK 484
             +ELSWNCGEEG T   +VLE RLKQIRNFLFIL++SLGVPV+NMGDECGQS+GG     
Sbjct: 662  ASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSP--A 719

Query: 483  YSDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPD 304
            YSDRK F+W AL+TGFATQTTQFI+FLS+ + RRSDL Q   FLK ENI WY  + TPP 
Sbjct: 720  YSDRKAFDWNALETGFATQTTQFIAFLSSFRNRRSDLLQKRNFLKEENIYWYESDQTPPR 779

Query: 303  WEDPTSKFLAMSLKVDQ-------ADKKLLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVR 145
            WEDP+ KFLAM LK D+        +     GD+FVAF+  D      +PPP   M W  
Sbjct: 780  WEDPSCKFLAMRLKADEDEVNQPGDESSRSRGDLFVAFSAADHSETVVLPPPLEGMGWRC 839

Query: 144  LVDTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSV 13
            LVDTALP+P FFS  GEPVVE++ G   YE+  HSC L E++S+
Sbjct: 840  LVDTALPFPGFFSTDGEPVVEQMVGLFAYEMKSHSCALFEARSL 883


>gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olitorius]
          Length = 868

 Score =  875 bits (2260), Expect = 0.0
 Identities = 432/703 (61%), Positives = 523/703 (74%), Gaps = 31/703 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSS--LNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++ELEF+A  APFY+SFLLK+S   N   +EI SHRKT FC P+G   GYP PLG+S
Sbjct: 168  SELALELEFEAKEAPFYLSFLLKASSDANSTGLEIRSHRKTSFCVPIGFDQGYPVPLGLS 227

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S DGS+NFA++SRNA++++LCL+DD+  E+PALE+DLDPYVNR+GDIWHASL     FV
Sbjct: 228  FSNDGSMNFAVYSRNAESLVLCLYDDTGSEKPALELDLDPYVNRTGDIWHASLEGAWTFV 287

Query: 1671 SYGYRCRNDAQNKSPFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSGDI 1528
            SYGYRC+ D  +      VLLDPY+K+IG +             LG +CKEP FDWSGD+
Sbjct: 288  SYGYRCKGDG-DAFHAERVLLDPYAKIIGSSIPNHYESGLLLKHLGRLCKEPAFDWSGDV 346

Query: 1527 RPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVP 1348
             PN+P+EKL+VYRLNVM FT D SS LP    GTF+GV EK+ H K LG++A+LLEPI  
Sbjct: 347  CPNLPLEKLVVYRLNVMRFTEDKSSKLPADVAGTFSGVTEKVQHLKYLGINAVLLEPIFT 406

Query: 1347 YDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDA 1168
            +D+QKGPYFP HFFSP + YGP  GS+S INS+K MVK LH+NGIEVLLEVVF+HT E  
Sbjct: 407  FDEQKGPYFPCHFFSPTSLYGPSNGSVSAINSIKGMVKNLHANGIEVLLEVVFTHTAEGG 466

Query: 1167 SLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM 1012
            +L  +D  SYY         +KN LNC +PVVQQ+ILDSLRHWV E+HIDGFCFINAS +
Sbjct: 467  ALQGLDDESYYYRNRVEDLEEKNALNCNYPVVQQMILDSLRHWVTEFHIDGFCFINASCL 526

Query: 1011 IRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFC 832
            +RGF GE+LSRPPL+EAIAFDP+LSKTK+IAD +DP     KEI FPHWKRWAEMNTKFC
Sbjct: 527  LRGFHGEHLSRPPLVEAIAFDPLLSKTKVIADCWDPHEMMPKEIHFPHWKRWAEMNTKFC 586

Query: 831  SDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK---T 661
            SD RN+LRGE  LS LATRLCGSGDIF  GRGPAFSFNFI  N G  LVDLVSFS     
Sbjct: 587  SDIRNFLRGEDALSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFSNAELA 646

Query: 660  TELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKY 481
            +ELSWNCGEEG T N +VLE RLKQIRN++FIL++SLGVPV+NMGDECGQSSGG   L Y
Sbjct: 647  SELSWNCGEEGPTSNTAVLERRLKQIRNYIFILFVSLGVPVLNMGDECGQSSGGS--LSY 704

Query: 480  SDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDW 301
              RK  +W A+ TGF  QTTQFISFLS+L+ RRSDL Q   FLK ENI+W+G + + P+W
Sbjct: 705  GSRKLLDWNAMTTGFGIQTTQFISFLSSLRERRSDLLQRRNFLKEENIEWHGSSQSQPEW 764

Query: 300  EDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            EDP+ KFLAM+LK D+A+  L      L GD+F+A N  D+     +PP    M W RLV
Sbjct: 765  EDPSCKFLAMTLKADKAESLLSSEASELKGDLFIAINADDKTENIILPPCPEGMAWRRLV 824

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSVD 10
            DTALPYP FF   G+ V+E++ G + YE+   SC L E+ + D
Sbjct: 825  DTALPYPGFFLADGKAVLEQMAGLVAYEMKSRSCTLFEACTED 867


>ref|XP_022771074.1| isoamylase 2, chloroplastic-like [Durio zibethinus]
 ref|XP_022771075.1| isoamylase 2, chloroplastic-like [Durio zibethinus]
          Length = 864

 Score =  874 bits (2257), Expect = 0.0
 Identities = 431/700 (61%), Positives = 523/700 (74%), Gaps = 32/700 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSS--LNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++ELEF+A  APFY SFLLK+S   N   +EI  HRKT FC P+G   G+PAPLG+S
Sbjct: 164  SELALELEFEAKEAPFYFSFLLKASSGANSTSLEIRGHRKTSFCVPIGFDQGHPAPLGLS 223

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S DGS+NFA++SRNA++++LCL+DD++ E+PA E+DLDPYVNR+GDIWHAS+     FV
Sbjct: 224  FSTDGSMNFAVYSRNAESLVLCLYDDTASEKPAFELDLDPYVNRTGDIWHASIEDAWTFV 283

Query: 1671 SYGYRCRNDAQNKS-PFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSGD 1531
            SYGYRC+ D  NK+     VLLDPY+K+IG +             LG++CKEP FDWSGD
Sbjct: 284  SYGYRCKGD--NKAFQADRVLLDPYAKIIGSSIPNHHESGLLPKHLGQLCKEPAFDWSGD 341

Query: 1530 IRPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIV 1351
            + PN+PMEKL+VYRLNVM FT+D SS LP    GTF+GV EK+ H KDLGV+A+LLEPI 
Sbjct: 342  VLPNLPMEKLVVYRLNVMRFTKDKSSKLPPNVAGTFSGVTEKVQHLKDLGVNAVLLEPIF 401

Query: 1350 PYDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCED 1171
             +D+QKGPYFP HFFSP N YGP  GSIS INS+KEMVK LH++GIEV LEVVF+HT E 
Sbjct: 402  TFDEQKGPYFPYHFFSPTNLYGPSNGSISAINSIKEMVKNLHADGIEVFLEVVFTHTAEG 461

Query: 1170 ASLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASS 1015
             +L  +D  SYY          +N LNC +PVVQQ+ILDSL+HWV E+HIDGFCFI+AS 
Sbjct: 462  GALQGLDDLSYYYRNRVRDLEARNALNCNYPVVQQMILDSLQHWVTEFHIDGFCFIDASC 521

Query: 1014 MIRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKF 835
            M+RGF GE+L RPPL+EAIAFDP+LS TKIIAD +DP     KEI+FPHWK+WAEMNTKF
Sbjct: 522  MLRGFYGEHLCRPPLVEAIAFDPLLSMTKIIADCWDPHDMMPKEIRFPHWKKWAEMNTKF 581

Query: 834  CSDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK--- 664
            C+D R +LRGE  LS LATRLCGSGDIF  GRGPAFSFNFI  N G  LVDLVSFS    
Sbjct: 582  CTDIRYFLRGEGALSSLATRLCGSGDIFSVGRGPAFSFNFIAKNFGLPLVDLVSFSNAEL 641

Query: 663  TTELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLK 484
             +E+SWNCGEEG T   +VLE RLKQIRNFLFIL++S GVPV+NMGDECGQSSGG   L 
Sbjct: 642  ASEISWNCGEEGPTSKTAVLERRLKQIRNFLFILFVSQGVPVLNMGDECGQSSGGS--LS 699

Query: 483  YSDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPD 304
            Y  RK  +W A++TG+  QTTQFISF S+L++RRSDL Q   FLK ENI+W+G N  PP 
Sbjct: 700  YGSRKLLDWDAMKTGYGIQTTQFISFFSSLRMRRSDLLQKRHFLKEENIEWHGSNQCPPG 759

Query: 303  WEDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRL 142
            WEDP+ KFLAM+LK D+A+ +L      L GD+F+A N  D+     +PPP   M W RL
Sbjct: 760  WEDPSCKFLAMTLKADKAESQLSSEASQLKGDLFIAINAADKAESLILPPPPEGMAWYRL 819

Query: 141  VDTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLES 22
            VDTALPYP FFSV G+ V E+  G + YE+ P SC L E+
Sbjct: 820  VDTALPYPGFFSVDGKAVREQKMGFVAYEMKPRSCTLFEA 859


>gb|KZN10097.1| hypothetical protein DCAR_002753 [Daucus carota subsp. sativus]
          Length = 757

 Score =  868 bits (2243), Expect = 0.0
 Identities = 432/697 (61%), Positives = 527/697 (75%), Gaps = 25/697 (3%)
 Frame = -2

Query: 2022 KLSVELEFDASLAPFYVSFLLK-SSLNGEKI-EIGSHRKTKFCFPVGLKSGYPAPLGVSY 1849
            K++VEL  +A+LAPFY+SF LK  S +G +I EI SHR T FC PVG  SGYPAPLG+S+
Sbjct: 66   KIAVELNLEANLAPFYLSFKLKFRSSDGLRISEIRSHRNTNFCVPVGFSSGYPAPLGLSF 125

Query: 1848 SADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVS 1669
            S DGS+NF+LFSR+A+ V+LCL+D S   EPALEIDLDPYVNR+GDIWHAS+ S +  +S
Sbjct: 126  STDGSMNFSLFSRSAERVVLCLYDTSMNAEPALEIDLDPYVNRTGDIWHASINSAIPLLS 185

Query: 1668 YGYRCRNDAQNKSPFSSVLLDPYSKVIGEN-------FLGEICKEPTFDWSGDIRPNVPM 1510
            YGY+C+   + K    +VLLDPY+K+IG N       +LG++ KEP FDW+GDIRP +P+
Sbjct: 186  YGYKCQAGTKQKK--DTVLLDPYAKIIGNNSSSLLPRYLGQLQKEPNFDWTGDIRPFLPL 243

Query: 1509 EKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKG 1330
            EKL+VYRLNV+ FT+D SS LPD   GTFTG+++KL HFKDLGV+ ILLEP  P++ Q+G
Sbjct: 244  EKLVVYRLNVLCFTKDQSSELPDDIAGTFTGISQKLQHFKDLGVNTILLEPFFPFNDQQG 303

Query: 1329 PYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKID 1150
            PY+P H FSP N YG     IS I S+KEMVK LH+NGIEV +EVVF+HT +  SL  ID
Sbjct: 304  PYYPFHLFSPANIYGHSKSPISNIKSVKEMVKTLHANGIEVFMEVVFTHTADTESLKDID 363

Query: 1149 KPSYYS-------DKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGE 991
              SY+         +NTLNC  PVVQQLILDSLRHWVIE+HIDGFCFINA+S++RG  GE
Sbjct: 364  NSSYHYVREYDGIHQNTLNCNFPVVQQLILDSLRHWVIEFHIDGFCFINAASLLRGCNGE 423

Query: 990  NLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYL 811
             L RPPL+EAIAFDPILS  KIIAD +DP     K ++FPHWKRWAE+NT+FC D RNYL
Sbjct: 424  TLFRPPLVEAIAFDPILSNVKIIADCWDPHDAEPKAVRFPHWKRWAEVNTRFCEDVRNYL 483

Query: 810  RGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKT---TELSWNC 640
            RGE+LLS+LATRLCGSGD F  GRGPAFSFNFI+ N G SLVDLVSFS +   +ELSWNC
Sbjct: 484  RGENLLSNLATRLCGSGDNFSAGRGPAFSFNFISRNSGLSLVDLVSFSASELASELSWNC 543

Query: 639  GEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFN 460
            G EGAT N  +LETRLKQ+RNFLFIL++SLGVPV+NMGDECGQSSGG     Y+DRKPFN
Sbjct: 544  GVEGATTNPDILETRLKQVRNFLFILFVSLGVPVLNMGDECGQSSGGSP--AYADRKPFN 601

Query: 459  WKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKF 280
            W +L+TGF  QTT+FISFLS+L++RRSDL Q   FLK ENI+W+G +L+PP WED  SKF
Sbjct: 602  WDSLKTGFGIQTTEFISFLSSLRVRRSDLLQKKNFLKEENIEWHGIDLSPPSWEDSESKF 661

Query: 279  LAMSLKV------DQADKKLLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYP 118
            L M LKV        +    + G++F+AFN  D      +PPP +DM W RLVDT+L YP
Sbjct: 662  LGMILKVYEEEIQSTSSAPSVNGNMFIAFNAADHSEDILLPPPPADMEWQRLVDTSLLYP 721

Query: 117  KFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSVDG 7
             FFS+ G+PV E++P  L YE+  HSC+L ES+   G
Sbjct: 722  GFFSLDGDPVFEQMP-ELVYEMKSHSCILFESRHTSG 757


>ref|XP_017978673.1| PREDICTED: isoamylase 2, chloroplastic [Theobroma cacao]
          Length = 867

 Score =  871 bits (2251), Expect = 0.0
 Identities = 432/703 (61%), Positives = 522/703 (74%), Gaps = 33/703 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLK--SSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++ELEF+A  APFY SFLLK  S  N    EI +HRKT FC P+G   GYPAPLG+S
Sbjct: 167  SELALELEFEAKEAPFYFSFLLKAPSGANSSGSEIRTHRKTNFCVPIGFNQGYPAPLGLS 226

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S DGS+NFA++SRNA++++LCL+DD++ E+PALE+DLDP+VNR+GDIWHAS+     FV
Sbjct: 227  FSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTFV 286

Query: 1671 SYGYRCRN--DAQNKSPFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSG 1534
             YGYRC+   DA N      VLLDPY+++IG +             LG +CKEP FDWSG
Sbjct: 287  RYGYRCKGYTDAFNAE---RVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSG 343

Query: 1533 DIRPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPI 1354
            D+ PN+PMEKL+VYRLNV+ FT D SS LP    GTF GV EK+ H KDLGV+A+LLEPI
Sbjct: 344  DVCPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPI 403

Query: 1353 VPYDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCE 1174
              +D+QKGPYFP HFFSP N YGP  GS+S INS+KEMVK LH+NG+EVLLEVVF+HT E
Sbjct: 404  FTFDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTAE 463

Query: 1173 DASLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINAS 1018
               L  +D  SYY          +N LNC +PVVQ++ILDSLRHWV E+HIDGFCFINAS
Sbjct: 464  GGGLQGLDDLSYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHIDGFCFINAS 523

Query: 1017 SMIRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTK 838
             ++RGF GE+LSRPPLIEAIAFDP+LSKTKIIAD + P     KEI+FPHWK+WAEMNTK
Sbjct: 524  CLLRGFYGEHLSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWKKWAEMNTK 583

Query: 837  FCSDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK-- 664
            FC+D RN+LRGE  LS LATRLCGSGDIF  GRGPAFSFN+I  N G  LVDLVSFSK  
Sbjct: 584  FCTDIRNFLRGEGALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVDLVSFSKAE 643

Query: 663  -TTELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYL 487
              +ELSWNCG EG T   +VLE RLKQIRNFLFIL+ISLGVPV+NMGDECGQSSGG   L
Sbjct: 644  IASELSWNCGVEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGS--L 701

Query: 486  KYSDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPP 307
             Y  RK  +W A+ TGF  QTTQFISFLS+L++RRSDL Q   FLK ENI+WYG + +PP
Sbjct: 702  SYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYGSDQSPP 761

Query: 306  DWEDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVR 145
             WEDP+ KFLAM+LK D+ + +L      L GD+ +A N  D+  I  +PPP   + W R
Sbjct: 762  GWEDPSCKFLAMTLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPEGLAWRR 821

Query: 144  LVDTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKS 16
            LVDTALPYP FFS  G+ V+E++ G   YE+   SC L E+++
Sbjct: 822  LVDTALPYPGFFSADGKAVLEQMMGLFAYEMKSLSCTLFEARA 864


>ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prunus mume]
          Length = 883

 Score =  871 bits (2250), Expect = 0.0
 Identities = 432/704 (61%), Positives = 523/704 (74%), Gaps = 34/704 (4%)
 Frame = -2

Query: 2022 KLSVELEFDASLAPFYVSFLLKS--SLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSY 1849
            + ++ELEF+A   PFY SF+L S    N   +EI SHRKT FC PVG   GYP PLG+++
Sbjct: 182  RFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTF 241

Query: 1848 SADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVS 1669
            S DGS+NFA+FSRNA++V+LCL+D+++ E+PALE+DLDPYVNRSGDIWH S GS   FVS
Sbjct: 242  SNDGSMNFAIFSRNAESVVLCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWTFVS 301

Query: 1668 YGYRCRNDA--QNKSPFSS--VLLDPYSKVI----------GENFLGEICKEPTFDWSGD 1531
            YGY+ + +    NK+ F    VLLDPY+KVI          G  +LG +C+EP FDW+GD
Sbjct: 302  YGYKFKRNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAGD 361

Query: 1530 IRPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIV 1351
            +RP++ MEKL+VYRLNV  FT   SS LP   GG+F+G+ EKL HFKDLGV+A+LLEPI 
Sbjct: 362  VRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPIF 421

Query: 1350 PYDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCED 1171
            P+D+QKGPYFP HFFSP    GP  G +S +NSMKEMV++ H+NGIEVLLEVVF+HT E 
Sbjct: 422  PFDEQKGPYFPRHFFSPMECIGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAEG 481

Query: 1170 ASLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASS 1015
             +L  ID  SYY          +N LNC +P+VQQL+LDSLR+WV E+HIDGFCFINASS
Sbjct: 482  EALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHIDGFCFINASS 541

Query: 1014 MIRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKF 835
            ++RGF GE LSRPPL+EAIAFDP+LSKTKIIAD +DP   + KE +FPHWKRWAE+NTKF
Sbjct: 542  LLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETRFPHWKRWAEVNTKF 601

Query: 834  CSDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFS---K 664
            C+D RNYLRG+ LLSDLATRLCG+GDIF  GRGPAF+FNFI+ N G  LVDLVSFS    
Sbjct: 602  CNDVRNYLRGKGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVEL 661

Query: 663  TTELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLK 484
             +ELSWNCGEEG T   +VLE RLKQIRNFLFIL++SLGVPV+N GDECGQS+GG     
Sbjct: 662  ASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNTGDECGQSTGGSP--A 719

Query: 483  YSDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPD 304
            YSDRK F+W AL+TGFATQTTQFI+FLS+ + RRSDL Q   FLK ENIDWY  + TPP 
Sbjct: 720  YSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIDWYESDQTPPR 779

Query: 303  WEDPTSKFLAMSLKVDQ-------ADKKLLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVR 145
            WEDP+ KFLAM LK D+        +     GD+F AF+  D      +PPPR  M W R
Sbjct: 780  WEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFAAFSAADHSETLVLPPPREGMGWRR 839

Query: 144  LVDTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSV 13
            LVDTALP+P FFS  GEPVV ++ G   YE+  HSC L E++S+
Sbjct: 840  LVDTALPFPGFFSTDGEPVVRQMVGLFAYEMKSHSCALFEARSL 883


>gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  870 bits (2247), Expect = 0.0
 Identities = 430/701 (61%), Positives = 521/701 (74%), Gaps = 31/701 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLK--SSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++ELEF+A  APFY SFLLK  S  N    EI +HRKT FC PVG   GYPAPLG+S
Sbjct: 167  SELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLGLS 226

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S DGS+NFA++SRNA++++LCL+DD++ E+PALE+DLDP+VNR+GDIWHAS+     FV
Sbjct: 227  FSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTFV 286

Query: 1671 SYGYRCRNDAQNKSPFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSGDI 1528
             YGYRC+ D    +    VLLDPY+++IG +             LG +CKEP FDWS D+
Sbjct: 287  RYGYRCKGDTDAFNA-ERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSSDV 345

Query: 1527 RPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVP 1348
             PN+PMEKL+VYRLNV+ FT D SS LP    GTF GV EK+ H KDLGV+A+LLEPI  
Sbjct: 346  CPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPIFT 405

Query: 1347 YDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDA 1168
            +D+QKGPYFP HFFSP N YGP  GS+S INS+KEMVK LH+NG+EVLLEVVF+HT E  
Sbjct: 406  FDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTAEGG 465

Query: 1167 SLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM 1012
            +L  +D  SYY          +N LNC +PVVQ++ILDSLRHWV E+HIDGFCFINAS +
Sbjct: 466  ALQGLDDLSYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHIDGFCFINASCL 525

Query: 1011 IRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFC 832
            +RGF GE+LSRPPLIEAIAFDP+LSKTKIIAD + P     KEI+FPHWK+WAEMNTKFC
Sbjct: 526  LRGFYGEHLSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWKKWAEMNTKFC 585

Query: 831  SDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK---T 661
            +D RN+LRGE  LS LATRLCGSGDIF  GRGPAFSFN+I  N G  LVDLVSFSK    
Sbjct: 586  TDIRNFLRGEGALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVDLVSFSKAEIA 645

Query: 660  TELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKY 481
            +ELSWNCG EG T   +VLE RLKQIRNFLFIL+ISLGVPV+NMGDECGQSSGG   L Y
Sbjct: 646  SELSWNCGVEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGS--LSY 703

Query: 480  SDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDW 301
              RK  +W A+ TGF  QTTQFISFLS+L++RRSDL Q   FLK ENI+WYG + +PP W
Sbjct: 704  GSRKLLDWNAMTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYGSDQSPPGW 763

Query: 300  EDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            EDP+ KFLAM+LK D+ + +L      L GD+ +A N  D+  I  +PPP   + W RLV
Sbjct: 764  EDPSCKFLAMTLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPEGLAWRRLV 823

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKS 16
            DTALPYP FFS  G+ V+E++ G   YE+   SC L E+++
Sbjct: 824  DTALPYPGFFSADGKAVLEQMMGLFAYEMKSLSCTLFEARA 864


>ref|XP_017230565.1| PREDICTED: isoamylase 2, chloroplastic [Daucus carota subsp. sativus]
 ref|XP_017230566.1| PREDICTED: isoamylase 2, chloroplastic [Daucus carota subsp. sativus]
          Length = 861

 Score =  868 bits (2243), Expect = 0.0
 Identities = 432/697 (61%), Positives = 527/697 (75%), Gaps = 25/697 (3%)
 Frame = -2

Query: 2022 KLSVELEFDASLAPFYVSFLLK-SSLNGEKI-EIGSHRKTKFCFPVGLKSGYPAPLGVSY 1849
            K++VEL  +A+LAPFY+SF LK  S +G +I EI SHR T FC PVG  SGYPAPLG+S+
Sbjct: 170  KIAVELNLEANLAPFYLSFKLKFRSSDGLRISEIRSHRNTNFCVPVGFSSGYPAPLGLSF 229

Query: 1848 SADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVS 1669
            S DGS+NF+LFSR+A+ V+LCL+D S   EPALEIDLDPYVNR+GDIWHAS+ S +  +S
Sbjct: 230  STDGSMNFSLFSRSAERVVLCLYDTSMNAEPALEIDLDPYVNRTGDIWHASINSAIPLLS 289

Query: 1668 YGYRCRNDAQNKSPFSSVLLDPYSKVIGEN-------FLGEICKEPTFDWSGDIRPNVPM 1510
            YGY+C+   + K    +VLLDPY+K+IG N       +LG++ KEP FDW+GDIRP +P+
Sbjct: 290  YGYKCQAGTKQKK--DTVLLDPYAKIIGNNSSSLLPRYLGQLQKEPNFDWTGDIRPFLPL 347

Query: 1509 EKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYDKQKG 1330
            EKL+VYRLNV+ FT+D SS LPD   GTFTG+++KL HFKDLGV+ ILLEP  P++ Q+G
Sbjct: 348  EKLVVYRLNVLCFTKDQSSELPDDIAGTFTGISQKLQHFKDLGVNTILLEPFFPFNDQQG 407

Query: 1329 PYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASLTKID 1150
            PY+P H FSP N YG     IS I S+KEMVK LH+NGIEV +EVVF+HT +  SL  ID
Sbjct: 408  PYYPFHLFSPANIYGHSKSPISNIKSVKEMVKTLHANGIEVFMEVVFTHTADTESLKDID 467

Query: 1149 KPSYYS-------DKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIRGFKGE 991
              SY+         +NTLNC  PVVQQLILDSLRHWVIE+HIDGFCFINA+S++RG  GE
Sbjct: 468  NSSYHYVREYDGIHQNTLNCNFPVVQQLILDSLRHWVIEFHIDGFCFINAASLLRGCNGE 527

Query: 990  NLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSDARNYL 811
             L RPPL+EAIAFDPILS  KIIAD +DP     K ++FPHWKRWAE+NT+FC D RNYL
Sbjct: 528  TLFRPPLVEAIAFDPILSNVKIIADCWDPHDAEPKAVRFPHWKRWAEVNTRFCEDVRNYL 587

Query: 810  RGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKT---TELSWNC 640
            RGE+LLS+LATRLCGSGD F  GRGPAFSFNFI+ N G SLVDLVSFS +   +ELSWNC
Sbjct: 588  RGENLLSNLATRLCGSGDNFSAGRGPAFSFNFISRNSGLSLVDLVSFSASELASELSWNC 647

Query: 639  GEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSDRKPFN 460
            G EGAT N  +LETRLKQ+RNFLFIL++SLGVPV+NMGDECGQSSGG     Y+DRKPFN
Sbjct: 648  GVEGATTNPDILETRLKQVRNFLFILFVSLGVPVLNMGDECGQSSGGSP--AYADRKPFN 705

Query: 459  WKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWEDPTSKF 280
            W +L+TGF  QTT+FISFLS+L++RRSDL Q   FLK ENI+W+G +L+PP WED  SKF
Sbjct: 706  WDSLKTGFGIQTTEFISFLSSLRVRRSDLLQKKNFLKEENIEWHGIDLSPPSWEDSESKF 765

Query: 279  LAMSLKV------DQADKKLLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLVDTALPYP 118
            L M LKV        +    + G++F+AFN  D      +PPP +DM W RLVDT+L YP
Sbjct: 766  LGMILKVYEEEIQSTSSAPSVNGNMFIAFNAADHSEDILLPPPPADMEWQRLVDTSLLYP 825

Query: 117  KFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSVDG 7
             FFS+ G+PV E++P  L YE+  HSC+L ES+   G
Sbjct: 826  GFFSLDGDPVFEQMP-ELVYEMKSHSCILFESRHTSG 861


>ref|XP_022758172.1| isoamylase 2, chloroplastic-like [Durio zibethinus]
          Length = 868

 Score =  868 bits (2242), Expect = 0.0
 Identities = 423/699 (60%), Positives = 520/699 (74%), Gaps = 31/699 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLKSS--LNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++E EF+A   P Y SFLLK+S   N  ++EI SHRKT FC P+G   GYPAPLG+S
Sbjct: 168  SELALEFEFEAKEVPCYFSFLLKASSGANSSRLEIRSHRKTSFCVPIGSDQGYPAPLGLS 227

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S+DGS+NFA++SRNA++++LCL+DD++ E+PALE+DLDPYVNR+GDIWHAS+     FV
Sbjct: 228  FSSDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPYVNRTGDIWHASIEGAWTFV 287

Query: 1671 SYGYRCRNDAQNKSPFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSGDI 1528
            SYGYRC+ D ++      VLLDPY+K+IG +             LG +CKEP FDWSGD+
Sbjct: 288  SYGYRCKGD-RDTFHAERVLLDPYAKIIGSSIPNHCESGLLLKHLGRLCKEPAFDWSGDV 346

Query: 1527 RPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVP 1348
             PN+ MEKL+VYRLNVM FT D SS LP    GTF+GV EK+ H KDLG++A+LLEPI  
Sbjct: 347  FPNIAMEKLVVYRLNVMRFTEDKSSKLPANVAGTFSGVTEKVQHLKDLGINAVLLEPIFT 406

Query: 1347 YDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDA 1168
            +D+ KGPYFP HFFSP N YGP  GS+S INS+KEMVK +H+NGIEV LEVVF+HT E  
Sbjct: 407  FDEHKGPYFPCHFFSPMNLYGPANGSVSAINSIKEMVKNMHANGIEVFLEVVFTHTAEGR 466

Query: 1167 SLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM 1012
            +L  +D  SYY          +N +NC +PVVQQ+ILDSLRHWV E+HIDGFCFINAS +
Sbjct: 467  ALQGLDDLSYYCRNNIEDLEARNAMNCNYPVVQQMILDSLRHWVTEFHIDGFCFINASCL 526

Query: 1011 IRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFC 832
            +RGF GE+LSRPPL+EAIAFDP+LS TKIIAD +DP +   KEI+FPHWK+WAEMN+KFC
Sbjct: 527  LRGFYGEHLSRPPLVEAIAFDPLLSMTKIIADCWDPQNMMPKEIRFPHWKKWAEMNSKFC 586

Query: 831  SDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK---T 661
            +D RN+LRGE  LS LATRLCGSGDIF  GRGPAFSFNFI  N G  LVDLVSF      
Sbjct: 587  TDIRNFLRGEGALSSLATRLCGSGDIFSDGRGPAFSFNFIAKNFGLPLVDLVSFCNAELA 646

Query: 660  TELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKY 481
            +E+SWNCGEEG T   +VLE RLKQIRNFLFIL++SLGVPV+NMGDECGQSSGG   L Y
Sbjct: 647  SEISWNCGEEGPTTKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSSGGS--LSY 704

Query: 480  SDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDW 301
              RK  +W A+ TGF  QTT+FISF S+L++RRSDL Q   FLK ENI+W G N +PP W
Sbjct: 705  DSRKFLDWNAMTTGFGIQTTKFISFFSSLRMRRSDLLQKRSFLKEENIEWQGINRSPPGW 764

Query: 300  EDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            +DP+ KFLAM+LK D+A+ +L      L GD+F+A N  D+     +PPP   M W RLV
Sbjct: 765  QDPSCKFLAMTLKADKAESQLSSEASQLKGDLFIAINAADKAESIILPPPLQGMSWRRLV 824

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLES 22
            DT+LPYP FFS  G+ V E+  G + YE+  HSC L E+
Sbjct: 825  DTSLPYPAFFSADGKAVPEQTTGLVAYEMKSHSCTLFEA 863


>ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]
 gb|ONH93660.1| hypothetical protein PRUPE_8G244800 [Prunus persica]
          Length = 883

 Score =  867 bits (2241), Expect = 0.0
 Identities = 431/704 (61%), Positives = 524/704 (74%), Gaps = 34/704 (4%)
 Frame = -2

Query: 2022 KLSVELEFDASLAPFYVSFLLKS--SLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSY 1849
            + ++ELEF+A   PFY SF+L S    N   +EI SHRKT FC PVG   GYP PLG+++
Sbjct: 182  RFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTF 241

Query: 1848 SADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVS 1669
            S DGS+NFA+FSRNA++V LCL+D+++ E+PALE+DLDPYVNRSGDIWH S GS  +FVS
Sbjct: 242  SNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFVS 301

Query: 1668 YGYRCRNDA--QNKSPFSS--VLLDPYSKVI----------GENFLGEICKEPTFDWSGD 1531
            YGY+ + +    NK+ F    VLLDPY+KVI          G  +LG +C+EP FDW+GD
Sbjct: 302  YGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAGD 361

Query: 1530 IRPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIV 1351
            +RP++ MEKL+VYRLNV  FT   SS LP   GG+F+G+ EKL HFKDLGV+A+LLEPI 
Sbjct: 362  VRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPIF 421

Query: 1350 PYDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCED 1171
            P+D+QKGPYFP HFFSP + +GP  G +S +NSMKEMV++ H+NGIEVLLEVVF+HT E 
Sbjct: 422  PFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAEG 481

Query: 1170 ASLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASS 1015
             +L  ID  SYY          +N LNC +P+VQQL+LDSLR+WV E+H+DGF FINASS
Sbjct: 482  EALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGFFFINASS 541

Query: 1014 MIRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKF 835
            ++RGF GE LSRPPL+EAIAFDP+LSKTKIIAD +DP   + KE  FPHW+RWAE+NTKF
Sbjct: 542  LLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRWAEVNTKF 601

Query: 834  CSDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFS---K 664
            C+D RN+LRGE LLSDLATRLCG+GDIF  GRGPAF+FNFI+ N G  LVDLVSFS    
Sbjct: 602  CNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVEL 661

Query: 663  TTELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLK 484
             +ELSWNCGEEG T   +VLE RLKQIRNFLFIL++SLGVPV+NMGDECGQS+GG     
Sbjct: 662  ASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSP--A 719

Query: 483  YSDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPD 304
            YSDRK F+W AL+TGFATQTTQFI+FLS+ + RRSDL Q   FLK ENI WY  + TPP 
Sbjct: 720  YSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESDQTPPR 779

Query: 303  WEDPTSKFLAMSLKVDQ-------ADKKLLTGDVFVAFNGGDEPVIGSVPPPRSDMVWVR 145
            WEDP+ KFLAM LK D+        +     GD+FVAF+  D      +PPP   M W R
Sbjct: 780  WEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEGMGWRR 839

Query: 144  LVDTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSV 13
            LVDTALP+P FFS  GEPVVE+I G   YE+  HSC L E++S+
Sbjct: 840  LVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARSL 883


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic [Fragaria vesca subsp. vesca]
          Length = 868

 Score =  865 bits (2236), Expect = 0.0
 Identities = 429/702 (61%), Positives = 520/702 (74%), Gaps = 34/702 (4%)
 Frame = -2

Query: 2016 SVELEFDASLAPFYVSFLLKS--SLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVSYSA 1843
            ++ELEF+A   PFY+SF +KS    N   +EI SHRKT FC PVG   G P PLG+SYS+
Sbjct: 169  TLELEFEAKQTPFYLSFTVKSLADANLSGLEIRSHRKTNFCVPVGFGRGCPTPLGLSYSS 228

Query: 1842 DGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSYG 1663
            DGSINFA+FSRNA++V+LCL+DD + +EPALE+DLDPYVNRSGDIWHAS  S   F+SYG
Sbjct: 229  DGSINFAIFSRNAESVVLCLYDDPTAQEPALELDLDPYVNRSGDIWHASFESAWTFLSYG 288

Query: 1662 YRCRNDA---QNKSPFSSVLLDPYSKVIGENF----------LGEICKEPTFDWSGDIRP 1522
            YR +  +    N     +VLLDPY+KVI E+           LG +C+EP FDW GD+RP
Sbjct: 289  YRFKGTSLRNTNSLDEGNVLLDPYAKVIDESIANNRGTGLKLLGRLCEEPVFDWDGDVRP 348

Query: 1521 NVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVPYD 1342
             +P+EKL+VYRLN+  FT   SS LP    GTF+G+ +KL HF+DLGV+AILLEPI P+D
Sbjct: 349  LLPIEKLVVYRLNLKRFTEHKSSKLPTNVAGTFSGLTQKLDHFQDLGVNAILLEPIFPFD 408

Query: 1341 KQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDASL 1162
            ++KGPYFP HFFSP N +GP GG I++INSMKEMVK  H+NGIEV+LEV+F+HT E   L
Sbjct: 409  EEKGPYFPCHFFSPMNCFGPSGGPIASINSMKEMVKEFHANGIEVILEVIFTHTAEGEVL 468

Query: 1161 TKIDKPSYYSDK--------NTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSMIR 1006
              ID  SYY           N+LNC HPVVQQ++LDSLR+WV E+HIDGFCFINASS++ 
Sbjct: 469  QGIDISSYYHASTTGDLEAGNSLNCNHPVVQQMVLDSLRYWVTEFHIDGFCFINASSLLH 528

Query: 1005 GFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFCSD 826
            G KGE  SRPPL+EAI FDP+L+KTK IAD +DP     KE +FPHWKRWAE+NT+FC+D
Sbjct: 529  GVKGEYQSRPPLVEAITFDPLLAKTKFIADCWDPHEMVPKETRFPHWKRWAEVNTRFCND 588

Query: 825  ARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKT---TE 655
             RN+LRGE LLSDLATRLCG+GDIF  GRGPAFSFNFIT N G  LVDLVSFS +   +E
Sbjct: 589  VRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFSFNFITRNSGLPLVDLVSFSGSELASE 648

Query: 654  LSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKYSD 475
            LSWNCGEEG T   +VLE RLKQIRNFLFILY+SLGVPV+NMGDECGQSSGG     YSD
Sbjct: 649  LSWNCGEEGPTSKTAVLERRLKQIRNFLFILYLSLGVPVLNMGDECGQSSGGSP--AYSD 706

Query: 474  RKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDWED 295
            RK F+WKAL+TGFATQ TQFI++LS+L+ RRSDL Q   F K ENIDWYG + + P WED
Sbjct: 707  RKSFDWKALETGFATQITQFIAYLSSLRRRRSDLLQKKHFFKEENIDWYGSDQSLPRWED 766

Query: 294  PTSKFLAMSLKVDQADKKL--------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            P  KFLA+ LK DQ + +         L GD+F+AF+  D+     +PPP+  M W RLV
Sbjct: 767  PLCKFLAVRLKADQDEVENQSNSVYAGLRGDLFLAFSAADQSETVILPPPQEGMAWSRLV 826

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSV 13
            DTALP+P FFS  GEPV+E++     YE+  HSC L E++S+
Sbjct: 827  DTALPFPGFFSTDGEPVIEQMKDLCAYEMKSHSCALFEARSL 868


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  866 bits (2237), Expect = 0.0
 Identities = 429/704 (60%), Positives = 533/704 (75%), Gaps = 34/704 (4%)
 Frame = -2

Query: 2016 SVELEFDASLAPFYVSFLLKSSLNGE--KIEIGSHRKTKFCFPVGLKSGYPAPLGVSYSA 1843
            +++L+F+A+ APFY+SFLLKS+L+ +    +I SHRKT FC PVG   GYPAPLG+S+S+
Sbjct: 183  ALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSFSS 242

Query: 1842 DGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFVSYG 1663
            DGS NFA FSRNA  V+LCL+D +S ++PALEIDLDPYVNR+GDIWHAS+ S  +FVSYG
Sbjct: 243  DGSPNFAFFSRNAGGVVLCLYDGTS-DKPALEIDLDPYVNRTGDIWHASMESVGSFVSYG 301

Query: 1662 YRCRN----DAQNKSPFSSVLLDPYSKVIGENF-----------LGEICKEPTFDWSGDI 1528
            YRC+     D+        V LDPY+K+I  +F           LGE+ KEP F+W+ D+
Sbjct: 302  YRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWNDDV 361

Query: 1527 RPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVP 1348
             P +PMEKL+VYRLNVM FT+D SS +     GTF+G+ EKLHHFKDLGV+A+LLEPI  
Sbjct: 362  HPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPIFS 421

Query: 1347 YDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDA 1168
            +D+QKGPYFP HFFSP N YGP  G +STINS+KEMVKRLH+NGIEVLLEVVF+HT E  
Sbjct: 422  FDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAESG 481

Query: 1167 SLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM 1012
            +L  ID   YY          +N LNC + +VQQ+I+DSLR+WV E+H+DGFCFINASS+
Sbjct: 482  ALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINASSL 541

Query: 1011 IRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFC 832
            +RGF GE LSRPPL+E IAFDP+LSKTKIIAD +DP +   KEI+FPHWKRWAE+NT+FC
Sbjct: 542  LRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTRFC 601

Query: 831  SDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSKT--- 661
            +D RN+LRGE  LSD ATRLCGSGDIF+ GRGPAFSFNF T N G  LVDLVSFS +   
Sbjct: 602  NDVRNFLRGEG-LSDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSELA 660

Query: 660  TELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKY 481
            +ELSWNCG+EG T   +VLE RLKQIRNFLFILY+SLGVP++NMGDECGQSSGG     Y
Sbjct: 661  SELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSP--AY 718

Query: 480  SDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDW 301
             DRKPFNW +++TGF  QT QFISFLS+L+ RRSDL Q   FLK E+IDW+G + +PP W
Sbjct: 719  GDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRW 778

Query: 300  EDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            +DP+SKFLAM+LK +  + +L      + GD+F+AFN  D  V   +PPP + MVW RLV
Sbjct: 779  DDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLV 838

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSVDG 7
            DTALP+P FF+  GE +++K  G +TY++  HSC L E+ ++DG
Sbjct: 839  DTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTLDG 882


>ref|XP_021281244.1| isoamylase 2, chloroplastic [Herrania umbratica]
 ref|XP_021281245.1| isoamylase 2, chloroplastic [Herrania umbratica]
          Length = 868

 Score =  865 bits (2235), Expect = 0.0
 Identities = 427/702 (60%), Positives = 521/702 (74%), Gaps = 31/702 (4%)
 Frame = -2

Query: 2025 NKLSVELEFDASLAPFYVSFLLK--SSLNGEKIEIGSHRKTKFCFPVGLKSGYPAPLGVS 1852
            ++L++ELEF+A  APFY SFLLK  S  N    EI +HRKT FC P+G   GYPAPLG+S
Sbjct: 168  HELALELEFEAKEAPFYFSFLLKAPSGANSSGSEIRTHRKTNFCVPIGFDQGYPAPLGLS 227

Query: 1851 YSADGSINFALFSRNAKNVILCLFDDSSKEEPALEIDLDPYVNRSGDIWHASLGSDMNFV 1672
            +S DGS+NFA++SRNA++++LCL+D+++ E+PALE+DLDPYVNR+GDIWHAS+   + FV
Sbjct: 228  FSTDGSMNFAVYSRNAESLVLCLYDNTASEKPALELDLDPYVNRTGDIWHASIEGALTFV 287

Query: 1671 SYGYRCRNDAQNKSPFSSVLLDPYSKVIGENF------------LGEICKEPTFDWSGDI 1528
            SYGYRC+ D    +    VLLDPY+K+IG +             LG +CKEP FDWSGD+
Sbjct: 288  SYGYRCKGDTDAFNA-ERVLLDPYAKIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSGDV 346

Query: 1527 RPNVPMEKLMVYRLNVMDFTRDASSNLPDGTGGTFTGVAEKLHHFKDLGVDAILLEPIVP 1348
             PN+PMEKL+VYRLNV+ FT D SS LP    GTF+GV EK+ H KDLG++A+LLEPI  
Sbjct: 347  CPNLPMEKLVVYRLNVLHFTEDKSSKLPADVAGTFSGVTEKVQHLKDLGINAVLLEPIFT 406

Query: 1347 YDKQKGPYFPSHFFSPQNSYGPPGGSISTINSMKEMVKRLHSNGIEVLLEVVFSHTCEDA 1168
            +D+QKGPYFP HFFSP N YGP  GS+S INS+KEMVK LH+NG+EVLLEVVF+HT E  
Sbjct: 407  FDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTAESG 466

Query: 1167 SLTKIDKPSYY--------SDKNTLNCAHPVVQQLILDSLRHWVIEYHIDGFCFINASSM 1012
            +L  +D  SYY           + LNC +PVVQQ+ILDSLRHW  E+HIDGFCFINAS +
Sbjct: 467  ALQGLDDLSYYYRNRVEDLEPGHALNCNYPVVQQMILDSLRHWATEFHIDGFCFINASCL 526

Query: 1011 IRGFKGENLSRPPLIEAIAFDPILSKTKIIADSFDPIHKSVKEIQFPHWKRWAEMNTKFC 832
            +RGF GE LSRPPLIEAIAFDP+LSKTKIIAD + P     KEI+FPHWK+WAEMNTKFC
Sbjct: 527  LRGFYGEILSRPPLIEAIAFDPLLSKTKIIADRWYPHDMMPKEIRFPHWKKWAEMNTKFC 586

Query: 831  SDARNYLRGESLLSDLATRLCGSGDIFLHGRGPAFSFNFITTNHGFSLVDLVSFSK---T 661
            +D RN+ RG+  LS LATRLCGSGDIF  GRGPAFSFNFI  N G  LVDLVSFS     
Sbjct: 587  TDIRNFWRGKGALSSLATRLCGSGDIFSDGRGPAFSFNFIAKNFGLPLVDLVSFSNAEIA 646

Query: 660  TELSWNCGEEGATKNKSVLETRLKQIRNFLFILYISLGVPVINMGDECGQSSGGKGYLKY 481
            +ELSWNCGEEG T   +VLE RLKQIRNFLFIL+IS GVPV+NMGDECGQSSGG   L Y
Sbjct: 647  SELSWNCGEEGPTSKTAVLERRLKQIRNFLFILFISRGVPVLNMGDECGQSSGGS--LSY 704

Query: 480  SDRKPFNWKALQTGFATQTTQFISFLSALKIRRSDLFQSNEFLKVENIDWYGPNLTPPDW 301
              RK  +W A+ TGF  QTT+FISFLS+L++RRSDL Q   FLK ENI+W+G + +PP W
Sbjct: 705  GSRKLLDWNAMTTGFGIQTTRFISFLSSLRMRRSDLLQKRNFLKEENIEWHGRDQSPPRW 764

Query: 300  EDPTSKFLAMSLKVDQADKKL------LTGDVFVAFNGGDEPVIGSVPPPRSDMVWVRLV 139
            EDP+ K LAM+LK D+ + +L      L GD+ +A N  D+  I  +PPP   M W RLV
Sbjct: 765  EDPSCKLLAMTLKADKEESQLSSEASQLKGDLLIAINAADKAEIIILPPPPEGMAWRRLV 824

Query: 138  DTALPYPKFFSVTGEPVVEKIPGSLTYELVPHSCVLLESKSV 13
            DTALPYP FFS  G+ V+E++ G + YE+   SC L E+++V
Sbjct: 825  DTALPYPGFFSADGKAVLEQMMGLVAYEMKSLSCTLFEARAV 866