BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00009959
         (2615 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_022006097.1| pullulanase 1, chloroplastic isoform X1 [Hel...  1379   0.0  
ref|XP_023751382.1| pullulanase 1, chloroplastic [Lactuca sativa...  1373   0.0  
ref|XP_022006098.1| pullulanase 1, chloroplastic isoform X2 [Hel...  1372   0.0  
gb|KVI09675.1| Alpha-1,6-glucosidase, pullulanase-type, C-termin...  1278   0.0  
ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Que...  1230   0.0  
ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Que...  1226   0.0  
ref|XP_009587790.1| PREDICTED: pullulanase 1, chloroplastic isof...  1220   0.0  
ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isof...  1220   0.0  
ref|XP_019236141.1| PREDICTED: pullulanase 1, chloroplastic isof...  1218   0.0  
ref|XP_006361707.1| PREDICTED: pullulanase 1, chloroplastic isof...  1214   0.0  
ref|XP_019066688.1| PREDICTED: pullulanase 1, chloroplastic isof...  1214   0.0  
ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica...  1214   0.0  
ref|XP_019236140.1| PREDICTED: pullulanase 1, chloroplastic isof...  1214   0.0  
ref|XP_015056539.1| PREDICTED: pullulanase 1, chloroplastic isof...  1211   0.0  
ref|XP_016436612.1| PREDICTED: pullulanase 1, chloroplastic-like...  1210   0.0  
gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]  1209   0.0  
gb|PHT29890.1| Pullulanase 1, chloroplastic [Capsicum baccatum]      1209   0.0  
ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isof...  1207   0.0  
ref|XP_016443817.1| PREDICTED: pullulanase 1, chloroplastic-like...  1207   0.0  
ref|XP_016443816.1| PREDICTED: pullulanase 1, chloroplastic-like...  1207   0.0  

>ref|XP_022006097.1| pullulanase 1, chloroplastic isoform X1 [Helianthus annuus]
 gb|OTF99361.1| putative limit dextrinase [Helianthus annuus]
          Length = 952

 Score = 1379 bits (3569), Expect = 0.0
 Identities = 669/790 (84%), Positives = 718/790 (90%), Gaps = 11/790 (1%)
 Frame = +2

Query: 278  RDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQS 457
            +DSL YSRAYWVSRSIIAWD+DA GGSC LYSS+SA LSVAENGI+GYD N ELER DQ 
Sbjct: 69   KDSLRYSRAYWVSRSIIAWDLDAVGGSCFLYSSKSAALSVAENGIEGYDLNIELERCDQR 128

Query: 458  LPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDDM 637
            LP+NVRDKFPHI+DYKAFKVP TLDL SILKDQLAVA FD+SG+CI VTGLQLPGVIDD 
Sbjct: 129  LPENVRDKFPHIKDYKAFKVPATLDLKSILKDQLAVALFDSSGQCISVTGLQLPGVIDDF 188

Query: 638  FSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGPR 817
            FSYTGPLGA+FS+E + LYLWAPTAQ+VRA IYN P   E   + QL ESNGVWS N PR
Sbjct: 189  FSYTGPLGAVFSNEAIDLYLWAPTAQDVRAFIYNEPSGGEIALVAQLKESNGVWSGNVPR 248

Query: 818  SWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNLA 997
            SWEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLSGDGKRTFLVNLDSD++KP KWD+L 
Sbjct: 249  SWEGCYYVYEVSVYHPSTLQIEKCFANDPYARGLSGDGKRTFLVNLDSDDLKPHKWDDLG 308

Query: 998  DEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGLT 1177
            DEKP+IADF+DISIYELH+RDFSAND  V+PE RGGYLAFTS+ESAGVLHLKKLSDAGLT
Sbjct: 309  DEKPNIADFSDISIYELHVRDFSANDPNVHPEIRGGYLAFTSQESAGVLHLKKLSDAGLT 368

Query: 1178 HIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVLW 1357
            HIHLLPTFQF DV+D+KE WK VD EML+SLPPDSAEQQEYIT+IQNEDGYNWGYNPVLW
Sbjct: 369  HIHLLPTFQFDDVDDQKENWKFVDTEMLESLPPDSAEQQEYITSIQNEDGYNWGYNPVLW 428

Query: 1358 GVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIVP 1537
            GVPKGSYATNPNGP RI+EFR MVQALNR+GLRVVLDVVY+HLH SGP  DNSV DKIVP
Sbjct: 429  GVPKGSYATNPNGPSRIIEFRKMVQALNRIGLRVVLDVVYNHLHGSGPVGDNSVLDKIVP 488

Query: 1538 GYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKST 1717
            GYYLRRN DGFIENSTCVNNTASEHFMVDRLI+DDL+NWVVNYKIDGFRFDLMGH+MKST
Sbjct: 489  GYYLRRNTDGFIENSTCVNNTASEHFMVDRLIIDDLLNWVVNYKIDGFRFDLMGHLMKST 548

Query: 1718 MMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFNDR 1897
            MM+AKSVL NLSRD+D +DGSSIFLYGEGWDFGEVANNGRG+NASQ NL G+GIGSFNDR
Sbjct: 549  MMKAKSVLNNLSRDKDKIDGSSIFLYGEGWDFGEVANNGRGVNASQLNLGGTGIGSFNDR 608

Query: 1898 IRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKEF 2044
            IR A+L           GFLTGLSLQPNGHDHGTEA+AARILA+S DHIQVGMAANLK+F
Sbjct: 609  IRDALLGGSPFGHPLQQGFLTGLSLQPNGHDHGTEANAARILALSTDHIQVGMAANLKDF 668

Query: 2045 VLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDE 2224
            VLTNCDG +VKGSEI T GGSPVAYA+QPVETINYVSAHDNETLFDIISLKTPMEISVDE
Sbjct: 669  VLTNCDGQEVKGSEIPTYGGSPVAYATQPVETINYVSAHDNETLFDIISLKTPMEISVDE 728

Query: 2225 SCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVGL 2404
             C+INHLATS+IALSQGVPFFHCGDEILRSKSLD DSYNSGDWFNR+DFSYNSNNWGVGL
Sbjct: 729  RCRINHLATSIIALSQGVPFFHCGDEILRSKSLDRDSYNSGDWFNRIDFSYNSNNWGVGL 788

Query: 2405 PPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQER 2584
            PPKGKNEKSWP+IKPRLAS SFKP KSHI+SALENFQNLL IRYSS+LFRLRTANAIQER
Sbjct: 789  PPKGKNEKSWPLIKPRLASASFKPNKSHILSALENFQNLLLIRYSSVLFRLRTANAIQER 848

Query: 2585 VRFHNTCTLH 2614
            VRFHNT   H
Sbjct: 849  VRFHNTGPSH 858


>ref|XP_023751382.1| pullulanase 1, chloroplastic [Lactuca sativa]
 gb|PLY94815.1| hypothetical protein LSAT_2X103740 [Lactuca sativa]
          Length = 963

 Score = 1373 bits (3555), Expect = 0.0
 Identities = 668/786 (84%), Positives = 717/786 (91%), Gaps = 11/786 (1%)
 Frame = +2

Query: 278  RDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQS 457
            +DSLSYSRAYWVS SIIAW+VDA GGSC LYSSRSA LSV +NGI+G D + +LERHDQ 
Sbjct: 78   QDSLSYSRAYWVSTSIIAWNVDAVGGSCFLYSSRSAALSVTDNGIEGSDFSIKLERHDQR 137

Query: 458  LPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDDM 637
            LP+NVRDKFPHI+DY AFKVP +LD  SILKDQLAVAFFD+SG+CI VTGLQLPGV+DD+
Sbjct: 138  LPENVRDKFPHIQDYNAFKVPISLDPKSILKDQLAVAFFDSSGQCISVTGLQLPGVLDDI 197

Query: 638  FSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGPR 817
            FSY GPLGAIF +E VSL+LWAPTAQ+VRA IYNGP   E LEIV L ESNGVWSVNGPR
Sbjct: 198  FSYLGPLGAIFLNEVVSLHLWAPTAQDVRAFIYNGPSGGESLEIVNLKESNGVWSVNGPR 257

Query: 818  SWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNLA 997
            SWEGCYYVYEVSVYHPSTL+IEKCIANDPYARGLSGDGKRTFLVNLDSD +KPQKWD+LA
Sbjct: 258  SWEGCYYVYEVSVYHPSTLKIEKCIANDPYARGLSGDGKRTFLVNLDSDALKPQKWDDLA 317

Query: 998  DEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGLT 1177
            D+KP+I DF+DISIYELH+RDFSAND TV PEARGGYLAF+S++SAGVLHLKKLSDAGLT
Sbjct: 318  DKKPNIVDFSDISIYELHVRDFSANDPTVDPEARGGYLAFSSQDSAGVLHLKKLSDAGLT 377

Query: 1178 HIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVLW 1357
            HIHLLPTF FGDV+DEKEKWKSVDMEMLKSLPPDS EQQEYITAIQNEDGYNWGYNPVLW
Sbjct: 378  HIHLLPTFHFGDVDDEKEKWKSVDMEMLKSLPPDSPEQQEYITAIQNEDGYNWGYNPVLW 437

Query: 1358 GVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIVP 1537
            GVPKGSYATNPNG CRI+EFR MVQALN +GLRVVLDVVY+HLH +GP  DNSV DKIVP
Sbjct: 438  GVPKGSYATNPNGSCRIIEFRKMVQALNFIGLRVVLDVVYNHLHGNGPVGDNSVLDKIVP 497

Query: 1538 GYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKST 1717
            GYYLRRNADGFIENSTC+NNTASEHFMVDRLI+DDL+NW VNYKIDGFRFDLMGHMMKST
Sbjct: 498  GYYLRRNADGFIENSTCMNNTASEHFMVDRLIIDDLLNWAVNYKIDGFRFDLMGHMMKST 557

Query: 1718 MMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFNDR 1897
            M RAKSVLKNLSR+  GVDG  IF+YGEGWDFGEVANNGRG+NASQ NLAG+GIGSFNDR
Sbjct: 558  MTRAKSVLKNLSRENGGVDGPRIFIYGEGWDFGEVANNGRGVNASQFNLAGTGIGSFNDR 617

Query: 1898 IRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKEF 2044
            IR A+L           GFLTGLSLQPNG DHGTE++AAR L +SKDHIQ+GMAANLKEF
Sbjct: 618  IRDALLGGSPFGHPLQQGFLTGLSLQPNGLDHGTESNAARALDVSKDHIQIGMAANLKEF 677

Query: 2045 VLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDE 2224
            VLTNCDG +VKGSEILT GG PVAY  QPVETINYVSAHDNETLFDIISLK PMEIS+DE
Sbjct: 678  VLTNCDGQEVKGSEILTYGGDPVAYTLQPVETINYVSAHDNETLFDIISLKAPMEISIDE 737

Query: 2225 SCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVGL 2404
             C+INHLATS+IALSQGVPFFHCGDEILRSKSLD DSYNSGDWFNRLDFSYNSNNWGVGL
Sbjct: 738  RCRINHLATSIIALSQGVPFFHCGDEILRSKSLDRDSYNSGDWFNRLDFSYNSNNWGVGL 797

Query: 2405 PPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQER 2584
            PPK KNEKSWPIIKPRLASPSFKP K+HI+SALENFQNLLRIRYSSILFRLRTANAIQER
Sbjct: 798  PPKEKNEKSWPIIKPRLASPSFKPHKNHILSALENFQNLLRIRYSSILFRLRTANAIQER 857

Query: 2585 VRFHNT 2602
            VRFHNT
Sbjct: 858  VRFHNT 863


>ref|XP_022006098.1| pullulanase 1, chloroplastic isoform X2 [Helianthus annuus]
          Length = 951

 Score = 1372 bits (3552), Expect = 0.0
 Identities = 668/790 (84%), Positives = 717/790 (90%), Gaps = 11/790 (1%)
 Frame = +2

Query: 278  RDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQS 457
            +DSL YSRAYWVSRSIIAWD+DA GGSC LYSS+SA LSVAENGI+GYD N ELER DQ 
Sbjct: 69   KDSLRYSRAYWVSRSIIAWDLDAVGGSCFLYSSKSAALSVAENGIEGYDLNIELERCDQR 128

Query: 458  LPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDDM 637
            LP+NVRDKFPHI+DYKAFKVP TLDL SILKDQLAVA FD+SG+CI VTGLQLPGVIDD 
Sbjct: 129  LPENVRDKFPHIKDYKAFKVPATLDLKSILKDQLAVALFDSSGQCISVTGLQLPGVIDDF 188

Query: 638  FSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGPR 817
            FSYTGPLGA+FS+E + LYLWAPTAQ+VRA IYN P   E   + QL ESNGVWS N PR
Sbjct: 189  FSYTGPLGAVFSNEAIDLYLWAPTAQDVRAFIYNEPSGGEIALVAQLKESNGVWSGNVPR 248

Query: 818  SWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNLA 997
            SWEGCYYVYEVSVYHPSTL+IEKC ANDPYARGLSGDGKRTFLVNLDSD++KP KWD+L 
Sbjct: 249  SWEGCYYVYEVSVYHPSTLQIEKCFANDPYARGLSGDGKRTFLVNLDSDDLKPHKWDDLG 308

Query: 998  DEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGLT 1177
            DEKP+IADF+DISIYELH+RDFSAND  V+PE RGGYLAFTS+ SAGVLHLKKLSDAGLT
Sbjct: 309  DEKPNIADFSDISIYELHVRDFSANDPNVHPEIRGGYLAFTSQ-SAGVLHLKKLSDAGLT 367

Query: 1178 HIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVLW 1357
            HIHLLPTFQF DV+D+KE WK VD EML+SLPPDSAEQQEYIT+IQNEDGYNWGYNPVLW
Sbjct: 368  HIHLLPTFQFDDVDDQKENWKFVDTEMLESLPPDSAEQQEYITSIQNEDGYNWGYNPVLW 427

Query: 1358 GVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIVP 1537
            GVPKGSYATNPNGP RI+EFR MVQALNR+GLRVVLDVVY+HLH SGP  DNSV DKIVP
Sbjct: 428  GVPKGSYATNPNGPSRIIEFRKMVQALNRIGLRVVLDVVYNHLHGSGPVGDNSVLDKIVP 487

Query: 1538 GYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKST 1717
            GYYLRRN DGFIENSTCVNNTASEHFMVDRLI+DDL+NWVVNYKIDGFRFDLMGH+MKST
Sbjct: 488  GYYLRRNTDGFIENSTCVNNTASEHFMVDRLIIDDLLNWVVNYKIDGFRFDLMGHLMKST 547

Query: 1718 MMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFNDR 1897
            MM+AKSVL NLSRD+D +DGSSIFLYGEGWDFGEVANNGRG+NASQ NL G+GIGSFNDR
Sbjct: 548  MMKAKSVLNNLSRDKDKIDGSSIFLYGEGWDFGEVANNGRGVNASQLNLGGTGIGSFNDR 607

Query: 1898 IRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKEF 2044
            IR A+L           GFLTGLSLQPNGHDHGTEA+AARILA+S DHIQVGMAANLK+F
Sbjct: 608  IRDALLGGSPFGHPLQQGFLTGLSLQPNGHDHGTEANAARILALSTDHIQVGMAANLKDF 667

Query: 2045 VLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDE 2224
            VLTNCDG +VKGSEI T GGSPVAYA+QPVETINYVSAHDNETLFDIISLKTPMEISVDE
Sbjct: 668  VLTNCDGQEVKGSEIPTYGGSPVAYATQPVETINYVSAHDNETLFDIISLKTPMEISVDE 727

Query: 2225 SCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVGL 2404
             C+INHLATS+IALSQGVPFFHCGDEILRSKSLD DSYNSGDWFNR+DFSYNSNNWGVGL
Sbjct: 728  RCRINHLATSIIALSQGVPFFHCGDEILRSKSLDRDSYNSGDWFNRIDFSYNSNNWGVGL 787

Query: 2405 PPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQER 2584
            PPKGKNEKSWP+IKPRLAS SFKP KSHI+SALENFQNLL IRYSS+LFRLRTANAIQER
Sbjct: 788  PPKGKNEKSWPLIKPRLASASFKPNKSHILSALENFQNLLLIRYSSVLFRLRTANAIQER 847

Query: 2585 VRFHNTCTLH 2614
            VRFHNT   H
Sbjct: 848  VRFHNTGPSH 857


>gb|KVI09675.1| Alpha-1,6-glucosidase, pullulanase-type, C-terminal [Cynara
            cardunculus var. scolymus]
          Length = 908

 Score = 1278 bits (3308), Expect = 0.0
 Identities = 635/786 (80%), Positives = 681/786 (86%), Gaps = 11/786 (1%)
 Frame = +2

Query: 278  RDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQS 457
            +DSLSYSRAYWVSRS+IAW+VDA GGSC LYSS SA L++AE GI+GYD N ELER+DQ 
Sbjct: 74   KDSLSYSRAYWVSRSVIAWNVDAVGGSCFLYSSSSAALTIAETGIEGYDLNIELERYDQR 133

Query: 458  LPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDDM 637
            LP+NVRDKFPHIRDYKAFKVP  LD  +ILKDQLAVAFFDA+G+CI VTGLQLPGV+DD+
Sbjct: 134  LPENVRDKFPHIRDYKAFKVPTALDPKTILKDQLAVAFFDANGQCISVTGLQLPGVLDDL 193

Query: 638  FSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGPR 817
            FSYTGPLGAIFS++ VSL+LWAPTAQ VRALIY+GP   E LEIVQL ES+GVWSVNGPR
Sbjct: 194  FSYTGPLGAIFSNKAVSLFLWAPTAQEVRALIYSGPSGGEALEIVQLKESDGVWSVNGPR 253

Query: 818  SWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNLA 997
            SWEGCYYVYEVSVYHPSTL+IEKCIANDPYARGLSGDGKRTFL NLDSD +KPQ W NLA
Sbjct: 254  SWEGCYYVYEVSVYHPSTLKIEKCIANDPYARGLSGDGKRTFLANLDSDALKPQAWGNLA 313

Query: 998  DEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGLT 1177
            DEKP+IADF+D+SIYELHIRDFS ND TV+PE RGGYLAFTS++S GVLHLKKLSDAGLT
Sbjct: 314  DEKPEIADFSDVSIYELHIRDFSVNDPTVHPEVRGGYLAFTSQDSNGVLHLKKLSDAGLT 373

Query: 1178 HIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVLW 1357
            HIHLLP FQF DV+DEK+KWK V          DSAEQQEYITAIQ+EDGYNWGYNPVLW
Sbjct: 374  HIHLLPAFQFADVDDEKDKWKFV----------DSAEQQEYITAIQDEDGYNWGYNPVLW 423

Query: 1358 GVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIVP 1537
            GVPKGSYAT+PNGPCRIMEFR MVQALNRLGLRVVLDVVY+HLH SGP  DNSV DK   
Sbjct: 424  GVPKGSYATDPNGPCRIMEFRKMVQALNRLGLRVVLDVVYNHLHGSGPIADNSVLDK--- 480

Query: 1538 GYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKST 1717
                         NSTCVNNTASEHFMVDRLIVDDL+NW  NYK                
Sbjct: 481  -------------NSTCVNNTASEHFMVDRLIVDDLLNWACNYK---------------- 511

Query: 1718 MMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFNDR 1897
             MRAKS+L+NLSRD+D VDGSSIF+YGEGWDFGEVANNGRGINASQ NLAGSGIGSFNDR
Sbjct: 512  -MRAKSLLRNLSRDKDEVDGSSIFMYGEGWDFGEVANNGRGINASQFNLAGSGIGSFNDR 570

Query: 1898 IRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKEF 2044
            IR A+L           GFLTGLSLQPNGHDHGTEA+AA  LA+SKDHIQVGMAANLK+F
Sbjct: 571  IRDALLGGSPFGHPLQQGFLTGLSLQPNGHDHGTEANAAHTLAVSKDHIQVGMAANLKDF 630

Query: 2045 VLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDE 2224
            VLTNCDG +VKGSE+LT GGSPV YASQPVETINYVSAHDNETLFDIISLKTPMEISVDE
Sbjct: 631  VLTNCDGQEVKGSEVLTYGGSPVGYASQPVETINYVSAHDNETLFDIISLKTPMEISVDE 690

Query: 2225 SCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVGL 2404
             C+INHLATSVIALSQGVPFFHCGDEILRSKS+D DSYNSGDWFNRLDFSY+SNNWGVGL
Sbjct: 691  RCRINHLATSVIALSQGVPFFHCGDEILRSKSMDRDSYNSGDWFNRLDFSYSSNNWGVGL 750

Query: 2405 PPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQER 2584
            PPKGKNE SWPIIKPRLASP FKPQKSHI+SALENFQNLLRIRYSSIL RLRTANAIQER
Sbjct: 751  PPKGKNENSWPIIKPRLASPLFKPQKSHILSALENFQNLLRIRYSSILLRLRTANAIQER 810

Query: 2585 VRFHNT 2602
            VRFHNT
Sbjct: 811  VRFHNT 816


>ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Quercus suber]
 gb|POE76573.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 963

 Score = 1230 bits (3182), Expect = 0.0
 Identities = 582/811 (71%), Positives = 690/811 (85%), Gaps = 11/811 (1%)
 Frame = +2

Query: 203  PNNIETLLLSFYSKNLKMTQPQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRS 382
            PN+    L    S +++ + P ++ +DSL +SRAYWVS+S IAW+VD G GSC L++S++
Sbjct: 57   PNSAHLSLRCSSSMSVENSTPTSQLQDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKT 116

Query: 383  ATLSVAENGIQGYDSNFELERHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLA 562
            A LS+ ++G++G D   +LE     LP+NV  KFPHI+DY+AFKVP TLD  ++LK QLA
Sbjct: 117  AALSIVDDGVEGKDMQIKLEEDSCGLPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLA 176

Query: 563  VAFFDASGRCIGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNG 742
            VA F++ G+CI VTGLQLPGV+DD+FSY GPLGA++S+E+VSLYLWAPTAQ V A IY  
Sbjct: 177  VATFNSDGKCINVTGLQLPGVLDDLFSYNGPLGALYSEESVSLYLWAPTAQAVCACIYRD 236

Query: 743  PYVVEPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLS 922
            P     LEIVQL E NGVWS  GP+ WEGCYYVYEVSVYHPSTLRI+KC  NDPYARGLS
Sbjct: 237  PVGGNALEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLS 296

Query: 923  GDGKRTFLVNLDSDNVKPQKWDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARG 1102
             DG+RTFLVNLDSD +KP++WDNLA+EKPDI  F+DISIYELHIRDFSAND TV+PE RG
Sbjct: 297  SDGRRTFLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRG 356

Query: 1103 GYLAFTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDS 1282
            GYLAFT ++S+GVLHLKKLS+AGLTH+HLLPTFQF  V+DEKE WK VD  ML+ LPPDS
Sbjct: 357  GYLAFTLQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDS 416

Query: 1283 AEQQEYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVV 1462
             +QQ+ ITAIQ+ DGYNWGYNPVLWGVPKGSYA+NPNGP RI+EFR M+QALNR+GLR+V
Sbjct: 417  VQQQDLITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIV 476

Query: 1463 LDVVYSHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDD 1642
            LDVVY+H+H SGP D+NSV DKIVPGYYLR+N DG IENSTCVNNTASEH MV+RLI+DD
Sbjct: 477  LDVVYNHVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDD 536

Query: 1643 LINWVVNYKIDGFRFDLMGHMMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEV 1822
            L++W V+YK+DGFRFDLMGH+MKSTM++AK  L +L+++ DG+DGSSI++YGEGWDFGEV
Sbjct: 537  LLHWAVHYKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIYIYGEGWDFGEV 596

Query: 1823 ANNGRGINASQSNLAGSGIGSFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTE 1969
            A NGRG+NASQ NL+G+ IGSFNDRIR A+L           GF+TGL LQPNGHDHGTE
Sbjct: 597  AKNGRGVNASQFNLSGTQIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNGHDHGTE 656

Query: 1970 ADAARILAISKDHIQVGMAANLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINY 2149
             +A  +LA++KDHIQVGMAANL++FVLTN +G +VKGSE+LT GG+PVAYA  P ET+NY
Sbjct: 657  TNAECMLAVAKDHIQVGMAANLRDFVLTNFEGKEVKGSEVLTYGGAPVAYALSPTETVNY 716

Query: 2150 VSAHDNETLFDIISLKTPMEISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDS 2329
            VSAHDNETLFDI+SLKTP EISVD+ C+INHLATSVIALSQG+PFFH GDE+LRSKSLD 
Sbjct: 717  VSAHDNETLFDIVSLKTPTEISVDKRCRINHLATSVIALSQGIPFFHSGDEMLRSKSLDR 776

Query: 2330 DSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALEN 2509
            DSYNSGDWFNRLDF+YNSNNWGVGLPPK KNEK+WP+IKPRLA PSFKPQK HI++A++N
Sbjct: 777  DSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKIHILAAVDN 836

Query: 2510 FQNLLRIRYSSILFRLRTANAIQERVRFHNT 2602
            F NLL IRYSS LFRL+TANAIQERVRFHNT
Sbjct: 837  FLNLLCIRYSSPLFRLKTANAIQERVRFHNT 867


>ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Quercus suber]
 gb|POE76574.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 961

 Score = 1226 bits (3173), Expect = 0.0
 Identities = 583/811 (71%), Positives = 689/811 (84%), Gaps = 11/811 (1%)
 Frame = +2

Query: 203  PNNIETLLLSFYSKNLKMTQPQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRS 382
            PN+    L    S +++ + P   S+DSL +SRAYWVS+S IAW+VD G GSC L++S++
Sbjct: 57   PNSAHLSLRCSSSMSVENSTPT--SQDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKT 114

Query: 383  ATLSVAENGIQGYDSNFELERHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLA 562
            A LS+ ++G++G D   +LE     LP+NV  KFPHI+DY+AFKVP TLD  ++LK QLA
Sbjct: 115  AALSIVDDGVEGKDMQIKLEEDSCGLPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLA 174

Query: 563  VAFFDASGRCIGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNG 742
            VA F++ G+CI VTGLQLPGV+DD+FSY GPLGA++S+E+VSLYLWAPTAQ V A IY  
Sbjct: 175  VATFNSDGKCINVTGLQLPGVLDDLFSYNGPLGALYSEESVSLYLWAPTAQAVCACIYRD 234

Query: 743  PYVVEPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLS 922
            P     LEIVQL E NGVWS  GP+ WEGCYYVYEVSVYHPSTLRI+KC  NDPYARGLS
Sbjct: 235  PVGGNALEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLS 294

Query: 923  GDGKRTFLVNLDSDNVKPQKWDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARG 1102
             DG+RTFLVNLDSD +KP++WDNLA+EKPDI  F+DISIYELHIRDFSAND TV+PE RG
Sbjct: 295  SDGRRTFLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRG 354

Query: 1103 GYLAFTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDS 1282
            GYLAFT ++S+GVLHLKKLS+AGLTH+HLLPTFQF  V+DEKE WK VD  ML+ LPPDS
Sbjct: 355  GYLAFTLQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDS 414

Query: 1283 AEQQEYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVV 1462
             +QQ+ ITAIQ+ DGYNWGYNPVLWGVPKGSYA+NPNGP RI+EFR M+QALNR+GLR+V
Sbjct: 415  VQQQDLITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIV 474

Query: 1463 LDVVYSHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDD 1642
            LDVVY+H+H SGP D+NSV DKIVPGYYLR+N DG IENSTCVNNTASEH MV+RLI+DD
Sbjct: 475  LDVVYNHVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDD 534

Query: 1643 LINWVVNYKIDGFRFDLMGHMMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEV 1822
            L++W V+YK+DGFRFDLMGH+MKSTM++AK  L +L+++ DG+DGSSI++YGEGWDFGEV
Sbjct: 535  LLHWAVHYKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIYIYGEGWDFGEV 594

Query: 1823 ANNGRGINASQSNLAGSGIGSFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTE 1969
            A NGRG+NASQ NL+G+ IGSFNDRIR A+L           GF+TGL LQPNGHDHGTE
Sbjct: 595  AKNGRGVNASQFNLSGTQIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNGHDHGTE 654

Query: 1970 ADAARILAISKDHIQVGMAANLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINY 2149
             +A  +LA++KDHIQVGMAANL++FVLTN +G +VKGSE+LT GG+PVAYA  P ET+NY
Sbjct: 655  TNAECMLAVAKDHIQVGMAANLRDFVLTNFEGKEVKGSEVLTYGGAPVAYALSPTETVNY 714

Query: 2150 VSAHDNETLFDIISLKTPMEISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDS 2329
            VSAHDNETLFDI+SLKTP EISVD+ C+INHLATSVIALSQG+PFFH GDE+LRSKSLD 
Sbjct: 715  VSAHDNETLFDIVSLKTPTEISVDKRCRINHLATSVIALSQGIPFFHSGDEMLRSKSLDR 774

Query: 2330 DSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALEN 2509
            DSYNSGDWFNRLDF+YNSNNWGVGLPPK KNEK+WP+IKPRLA PSFKPQK HI++A++N
Sbjct: 775  DSYNSGDWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKIHILAAVDN 834

Query: 2510 FQNLLRIRYSSILFRLRTANAIQERVRFHNT 2602
            F NLL IRYSS LFRL+TANAIQERVRFHNT
Sbjct: 835  FLNLLCIRYSSPLFRLKTANAIQERVRFHNT 865


>ref|XP_009587790.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana
            tomentosiformis]
          Length = 927

 Score = 1220 bits (3157), Expect = 0.0
 Identities = 584/792 (73%), Positives = 676/792 (85%), Gaps = 11/792 (1%)
 Frame = +2

Query: 263  PQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELE 442
            P + S+DSL YSRA+WVSRSIIAW+V AG G C LY+SR+A L VA+  IQG+D   +LE
Sbjct: 66   PSSCSQDSLFYSRAFWVSRSIIAWNVGAGEGECYLYASRNAALCVADGEIQGHDVKIKLE 125

Query: 443  RHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPG 622
            R +  L Q V +KFPHI+DY+AF+VP TL+  S++K QLAV  F++ G+C   TGLQLPG
Sbjct: 126  RTNYGLSQQVVEKFPHIQDYRAFEVPATLNCQSLVKCQLAVGIFNSDGKCASATGLQLPG 185

Query: 623  VIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWS 802
            ++D++FSYTGPLGA+F  E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E NGVWS
Sbjct: 186  ILDELFSYTGPLGAVFDTEAVSLYLWAPTAQEVRALIYKSPSEADPIEIVQLKELNGVWS 245

Query: 803  VNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQK 982
            V GPR WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKR  LVNLDSD+VKP+ 
Sbjct: 246  VKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLDSDDVKPEG 305

Query: 983  WDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLS 1162
            WDNL DEKP++  F+DIS+YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS
Sbjct: 306  WDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLS 365

Query: 1163 DAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGY 1342
             AG+TH+HLLPTFQF  VEDEK KWK VD+E L S PPDS EQQ  ITAIQNEDGYNWGY
Sbjct: 366  SAGITHVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGY 425

Query: 1343 NPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVY 1522
            NPVLWGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+H++ASG  DD SV 
Sbjct: 426  NPVLWGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVLDVVYNHINASGHFDDFSVL 485

Query: 1523 DKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGH 1702
            DKIVPGYYLRRNADG IE+STCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH
Sbjct: 486  DKIVPGYYLRRNADGGIEHSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGH 545

Query: 1703 MMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIG 1882
            +MK TM++AKS+L +LS+D++GVDG SI++YGEGWDFGEVANNGRGINASQ NL GSGIG
Sbjct: 546  IMKHTMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIG 605

Query: 1883 SFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAA 2029
            SFNDRIR A+L           GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAA
Sbjct: 606  SFNDRIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAA 665

Query: 2030 NLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPME 2209
            NLK+FVL+NCDG +VKGSE+L     PV YAS P+ET+NYVSAHDNETLFDIISLKTP +
Sbjct: 666  NLKDFVLSNCDGEEVKGSEVLLHDRKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKD 725

Query: 2210 ISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNN 2389
            ISV+E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNN
Sbjct: 726  ISVEERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNN 785

Query: 2390 WGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTAN 2569
            WGVGLPPK KNE +WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN
Sbjct: 786  WGVGLPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTAN 845

Query: 2570 AIQERVRFHNTC 2605
            +IQERVRFHNTC
Sbjct: 846  SIQERVRFHNTC 857


>ref|XP_009587786.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
            tomentosiformis]
          Length = 952

 Score = 1220 bits (3157), Expect = 0.0
 Identities = 584/792 (73%), Positives = 676/792 (85%), Gaps = 11/792 (1%)
 Frame = +2

Query: 263  PQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELE 442
            P + S+DSL YSRA+WVSRSIIAW+V AG G C LY+SR+A L VA+  IQG+D   +LE
Sbjct: 66   PSSCSQDSLFYSRAFWVSRSIIAWNVGAGEGECYLYASRNAALCVADGEIQGHDVKIKLE 125

Query: 443  RHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPG 622
            R +  L Q V +KFPHI+DY+AF+VP TL+  S++K QLAV  F++ G+C   TGLQLPG
Sbjct: 126  RTNYGLSQQVVEKFPHIQDYRAFEVPATLNCQSLVKCQLAVGIFNSDGKCASATGLQLPG 185

Query: 623  VIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWS 802
            ++D++FSYTGPLGA+F  E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E NGVWS
Sbjct: 186  ILDELFSYTGPLGAVFDTEAVSLYLWAPTAQEVRALIYKSPSEADPIEIVQLKELNGVWS 245

Query: 803  VNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQK 982
            V GPR WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKR  LVNLDSD+VKP+ 
Sbjct: 246  VKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRALLVNLDSDDVKPEG 305

Query: 983  WDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLS 1162
            WDNL DEKP++  F+DIS+YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS
Sbjct: 306  WDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLS 365

Query: 1163 DAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGY 1342
             AG+TH+HLLPTFQF  VEDEK KWK VD+E L S PPDS EQQ  ITAIQNEDGYNWGY
Sbjct: 366  SAGITHVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGY 425

Query: 1343 NPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVY 1522
            NPVLWGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+H++ASG  DD SV 
Sbjct: 426  NPVLWGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVLDVVYNHINASGHFDDFSVL 485

Query: 1523 DKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGH 1702
            DKIVPGYYLRRNADG IE+STCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH
Sbjct: 486  DKIVPGYYLRRNADGGIEHSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGH 545

Query: 1703 MMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIG 1882
            +MK TM++AKS+L +LS+D++GVDG SI++YGEGWDFGEVANNGRGINASQ NL GSGIG
Sbjct: 546  IMKHTMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIG 605

Query: 1883 SFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAA 2029
            SFNDRIR A+L           GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAA
Sbjct: 606  SFNDRIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAA 665

Query: 2030 NLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPME 2209
            NLK+FVL+NCDG +VKGSE+L     PV YAS P+ET+NYVSAHDNETLFDIISLKTP +
Sbjct: 666  NLKDFVLSNCDGEEVKGSEVLLHDRKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKD 725

Query: 2210 ISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNN 2389
            ISV+E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNN
Sbjct: 726  ISVEERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNN 785

Query: 2390 WGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTAN 2569
            WGVGLPPK KNE +WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN
Sbjct: 786  WGVGLPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTAN 845

Query: 2570 AIQERVRFHNTC 2605
            +IQERVRFHNTC
Sbjct: 846  SIQERVRFHNTC 857


>ref|XP_019236141.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana
            attenuata]
 gb|OIT23986.1| pullulanase 1, chloroplastic [Nicotiana attenuata]
          Length = 955

 Score = 1218 bits (3152), Expect = 0.0
 Identities = 596/833 (71%), Positives = 690/833 (82%), Gaps = 15/833 (1%)
 Frame = +2

Query: 149  SYPRNRYMKQTSYLYLY*PN-NIET---LLLSFYSKNLKMTQPQARSRDSLSYSRAYWVS 316
            S+ +NR   Q S L  Y P  N+ T     L   S   + + P + S+DSL YSRA+WVS
Sbjct: 27   SHLKNRRRLQPSSLTRYRPGQNLGTSRPFTLRCSSTMHQSSAPSSCSQDSLFYSRAFWVS 86

Query: 317  RSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQSLPQNVRDKFPHIR 496
            RSIIAW+V AG G C LY+S++A L VA+  IQG+D   +LER +  LPQ V +KFPHIR
Sbjct: 87   RSIIAWNVGAGDGECYLYASKNAALCVADGEIQGHDVKIKLERTNYGLPQQVVEKFPHIR 146

Query: 497  DYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDDMFSYTGPLGAIFSD 676
            DY AF+VP TL+  S++K QLAV  F++ G+C   TGLQLPG++D++FSYTGPLGA+F  
Sbjct: 147  DYTAFEVPATLNCQSLVKCQLAVGIFNSDGKCASATGLQLPGILDELFSYTGPLGAVFDT 206

Query: 677  ENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSV 856
            E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E NGVWSV G R WEGCYYVYEVSV
Sbjct: 207  EAVSLYLWAPTAQEVRALIYKSPAEADPVEIVQLKELNGVWSVKGIRHWEGCYYVYEVSV 266

Query: 857  YHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNLADEKPDIADFADIS 1036
            YHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ WDNL DEKP++  F+DIS
Sbjct: 267  YHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNLQDEKPNLLSFSDIS 326

Query: 1037 IYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDV 1216
            +YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS AG+TH+HLLPTFQF  V
Sbjct: 327  LYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFAGV 386

Query: 1217 EDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVLWGVPKGSYATNPNG 1396
            EDEK KWK VD+E L S PPDS EQQ  ITAIQNEDGYNWGYNPVLWGVPKGSYA+N NG
Sbjct: 387  EDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWGVPKGSYASNANG 446

Query: 1397 PCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIVPGYYLRRNADGFIE 1576
            PCRI+EFR MVQALNR+GLRVV DVVY+H++ASG  DD SV DKIVPGYYLRRN DG IE
Sbjct: 447  PCRIIEFRKMVQALNRIGLRVVSDVVYNHINASGHFDDFSVLDKIVPGYYLRRNVDGGIE 506

Query: 1577 NSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKSTMMRAKSVLKNLSR 1756
            NSTCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH+MK TM++AKS+L +LS+
Sbjct: 507  NSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKRTMVKAKSLLNSLSK 566

Query: 1757 DEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFNDRIRGAVL------- 1915
            D++GVDG SI++YGEGWDFGEVAN+GRGINASQ NL GSGIGSFNDRIR A+L       
Sbjct: 567  DKNGVDGPSIYIYGEGWDFGEVANSGRGINASQFNLFGSGIGSFNDRIRDALLGGSPFGH 626

Query: 1916 ----GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKEFVLTNCDGHKVKGS 2083
                GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAANLK+FVLTNCDG +VKGS
Sbjct: 627  PLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLTNCDGEEVKGS 686

Query: 2084 EILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDESCKINHLATSVIA 2263
            E+L     PV YAS PVET+NYVSAHDNETLFDIISLKTP +ISV+E C++NHLATSVIA
Sbjct: 687  EVLLHDRKPVGYASSPVETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSVIA 746

Query: 2264 LSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPII 2443
            LSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNNWGVGLPPK KNE +WP+I
Sbjct: 747  LSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWPLI 806

Query: 2444 KPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNT 2602
            KPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN+IQERVRFHNT
Sbjct: 807  KPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNT 859


>ref|XP_006361707.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Solanum
            tuberosum]
          Length = 951

 Score = 1214 bits (3142), Expect = 0.0
 Identities = 580/787 (73%), Positives = 671/787 (85%), Gaps = 11/787 (1%)
 Frame = +2

Query: 275  SRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQ 454
            S+DSL YSRA+WV++SIIAW+V AG G C LY+SR A L VA +GIQG+D   +LER + 
Sbjct: 70   SQDSLFYSRAFWVTKSIIAWNVGAGEGECYLYASRKAELCVAGDGIQGHDVKIKLERTNY 129

Query: 455  SLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDD 634
             LPQ V +KFPHIRDY AF+VP TLD  S++K QLAV  F++ G+C   TGLQLPG++D+
Sbjct: 130  GLPQQVIEKFPHIRDYAAFEVPATLDFQSLVKCQLAVGIFNSYGKCASATGLQLPGILDE 189

Query: 635  MFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGP 814
            +FSYTGPLGA+F+ E VSLYLWAPTAQ V ALIY  P   +P+EIVQL E +GVWS  GP
Sbjct: 190  LFSYTGPLGAVFATEAVSLYLWAPTAQAVEALIYKSPSEADPVEIVQLKELDGVWSAKGP 249

Query: 815  RSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNL 994
              WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ WDNL
Sbjct: 250  MHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 309

Query: 995  ADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGL 1174
             DEKP++  F+D+SIYELH+RDFSA+D TV  E +GGYLAFTS++SAGV HLK+LS AG+
Sbjct: 310  QDEKPNLLSFSDVSIYELHVRDFSASDPTVSHEFQGGYLAFTSQDSAGVQHLKRLSSAGI 369

Query: 1175 THIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVL 1354
            TH+HLLPT+QF  VEDEK KWK  D+E L S PPDS EQQ  ITAIQ+EDGYNWGYNPVL
Sbjct: 370  THVHLLPTYQFAGVEDEKHKWKYTDIEKLNSFPPDSEEQQALITAIQDEDGYNWGYNPVL 429

Query: 1355 WGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIV 1534
            WGVPKGSYA N NGPCRI+EFR MVQALNR+GLRVVLDVVY+HLHASGP  D SV DKIV
Sbjct: 430  WGVPKGSYAGNANGPCRIVEFRKMVQALNRIGLRVVLDVVYNHLHASGPFSDFSVLDKIV 489

Query: 1535 PGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKS 1714
            PGY+LRRNADG IENSTCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH+MK 
Sbjct: 490  PGYFLRRNADGGIENSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKR 549

Query: 1715 TMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFND 1894
            TM++AKS+L +LS +E+GVDGSSI++YGEGWDFGEVA NGRGINASQ NL G+GIGSFND
Sbjct: 550  TMLKAKSLLNSLSTEENGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNLFGAGIGSFND 609

Query: 1895 RIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKE 2041
            RIR A+L           GF+TGL L+PNGHD G +A+  R+L +SKDHIQVGMAANLK+
Sbjct: 610  RIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLTVSKDHIQVGMAANLKD 669

Query: 2042 FVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVD 2221
            FVLTNCDG +VKGSE+L   G PV YAS PVET+NYVSAHDNETLFDIISLKTP +ISV+
Sbjct: 670  FVLTNCDGQEVKGSEVLMYDGKPVGYASSPVETVNYVSAHDNETLFDIISLKTPKDISVE 729

Query: 2222 ESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVG 2401
            E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNNWGVG
Sbjct: 730  ERCRMNHLATSVIALSQGIPFFHAGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVG 789

Query: 2402 LPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQE 2581
            +PPK KNE++WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRL+TANAIQE
Sbjct: 790  IPPKEKNERNWPLIKPRLADPSYKPQKSHILAAVENFLNLMQIRYSSPLFRLKTANAIQE 849

Query: 2582 RVRFHNT 2602
            RVRFHNT
Sbjct: 850  RVRFHNT 856


>ref|XP_019066688.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Solanum
            lycopersicum]
          Length = 951

 Score = 1214 bits (3142), Expect = 0.0
 Identities = 581/787 (73%), Positives = 671/787 (85%), Gaps = 11/787 (1%)
 Frame = +2

Query: 275  SRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQ 454
            S+DSL YSRA+WV++SIIAW+V AG G C LY+SR A L V ++GIQG+D   +LER + 
Sbjct: 70   SQDSLFYSRAFWVTKSIIAWNVGAGEGECYLYASRKAGLCVGDDGIQGHDVKIKLERTNY 129

Query: 455  SLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDD 634
             LPQ V +KFPHIRDY AF+VP TLD  S++K QLAV  F++ G+C   TGLQLPG++DD
Sbjct: 130  GLPQQVIEKFPHIRDYAAFEVPATLDFQSLVKCQLAVGIFNSYGKCASATGLQLPGILDD 189

Query: 635  MFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGP 814
            +FSYTGPLG IF+ E VSLYLWAPTAQ V+ALIY  P   +P+EIVQL E +GVWS  GP
Sbjct: 190  LFSYTGPLGGIFATEAVSLYLWAPTAQAVQALIYKSPSEADPVEIVQLKELDGVWSAKGP 249

Query: 815  RSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNL 994
            R WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ WDNL
Sbjct: 250  RHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 309

Query: 995  ADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGL 1174
             DEKP++  F+D+SIYELH+RDFSAND TV  E +GGYLAFTS++SAGV HLK+LS+AG+
Sbjct: 310  QDEKPNLLSFSDVSIYELHVRDFSANDPTVPHEFQGGYLAFTSQDSAGVQHLKRLSNAGI 369

Query: 1175 THIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVL 1354
            TH+HLLPT+QF  VEDEK KWK  D+E L S PPDS EQQ  ITAIQ+EDGYNWGYNPVL
Sbjct: 370  THVHLLPTYQFAGVEDEKHKWKYTDIEKLNSFPPDSEEQQALITAIQDEDGYNWGYNPVL 429

Query: 1355 WGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIV 1534
            WGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+HLHASGP  D SV DKIV
Sbjct: 430  WGVPKGSYASNANGPCRIVEFRKMVQALNRIGLRVVLDVVYNHLHASGPFSDFSVLDKIV 489

Query: 1535 PGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKS 1714
            PGY+LRRNADG IENSTCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH+MK 
Sbjct: 490  PGYFLRRNADGGIENSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKR 549

Query: 1715 TMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFND 1894
            TM++AKS L +LS +E+GVDGSSI++YGEGWDFGEVA NGRGINASQ NL G+GIGSFND
Sbjct: 550  TMLKAKSFLNSLSTEENGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNLFGAGIGSFND 609

Query: 1895 RIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKE 2041
            RIR A+L           GF+TGL  +PNGHD G +A+  R+L +SKDHIQVGMAANLK+
Sbjct: 610  RIRDALLGGSPFGHPLHQGFVTGLYSEPNGHDLGDKANVERMLTVSKDHIQVGMAANLKD 669

Query: 2042 FVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVD 2221
            FVLTNCDG +VKGSE+L     PV YAS PVET+NYVSAHDNETLFDIISLKTP +ISV+
Sbjct: 670  FVLTNCDGQEVKGSEVLMHDRKPVGYASSPVETVNYVSAHDNETLFDIISLKTPKDISVE 729

Query: 2222 ESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVG 2401
            E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNNWGVG
Sbjct: 730  ERCRMNHLATSVIALSQGIPFFHAGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVG 789

Query: 2402 LPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQE 2581
            LPPK KNE++WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRL+TANAIQE
Sbjct: 790  LPPKEKNERNWPLIKPRLADPSYKPQKSHILAAVENFLNLMQIRYSSPLFRLKTANAIQE 849

Query: 2582 RVRFHNT 2602
            RVRFHNT
Sbjct: 850  RVRFHNT 856


>ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica]
 gb|ONI23747.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 965

 Score = 1214 bits (3141), Expect = 0.0
 Identities = 583/805 (72%), Positives = 670/805 (83%), Gaps = 11/805 (1%)
 Frame = +2

Query: 221  LLLSFYSKNLKMTQPQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVA 400
            L  S  S  +   +  + S+ SL YSRA+WVS SIIAW+V  G GSC L +S++A LS+ 
Sbjct: 63   LQCSASSLYMSAEESTSTSQGSLLYSRAFWVSESIIAWNVYVGNGSCYLLASKTAALSLT 122

Query: 401  ENGIQGYDSNFELERHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDA 580
             +GI G D   +LE     LP+NV++KFPHI+DY+AF VP  LD   +LK QLAVA F++
Sbjct: 123  SDGILGEDVKVKLEEDKHGLPENVKEKFPHIKDYRAFNVPPDLDAKPLLKCQLAVATFNS 182

Query: 581  SGRCIGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEP 760
             GRC   TGLQLPG++DD+FSY GPLGA++S ++VSLYLWAPTAQ V   IY  P    P
Sbjct: 183  DGRCSDATGLQLPGILDDLFSYNGPLGALYSKQSVSLYLWAPTAQEVCVCIYKEPSGGSP 242

Query: 761  LEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRT 940
            LE+VQL E NGVWS  GP+SWEGCYYVYEVSVYHPSTLRIEKC ANDPYARGLS DG+RT
Sbjct: 243  LEVVQLEEFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRRT 302

Query: 941  FLVNLDSDNVKPQKWDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFT 1120
             LVNLDSDN+KP+ WD L DEKPDI  F+DISIYELHIRDFSA+D  V+PE RGGYLAFT
Sbjct: 303  LLVNLDSDNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEFRGGYLAFT 362

Query: 1121 SEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEY 1300
             ++SAG +HLKKLS+AG+TH+HLLP FQF  V+DEKE WKSVD E+L+  P DS +QQ  
Sbjct: 363  LQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVDPEVLEKFPSDSDKQQAL 422

Query: 1301 ITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYS 1480
            ITAIQN+DGYNWGYNPVLWGVPKGSYA+N NG  R +EFR MVQALNR GLRVVLDVVY+
Sbjct: 423  ITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMVQALNRFGLRVVLDVVYN 482

Query: 1481 HLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVV 1660
            HLH SGP DDNSV DKIVPGYYLRRN DGFIE+STCVNNTASEHFMV+RLIVDDL++W V
Sbjct: 483  HLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHFMVERLIVDDLLHWAV 542

Query: 1661 NYKIDGFRFDLMGHMMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRG 1840
            +YK+DGFRFDLMGH+M+ TM++AK  L +L+++ DGVDGSSI++YGEGWDFGEVANNGRG
Sbjct: 543  DYKVDGFRFDLMGHIMRRTMVKAKDALCSLTKERDGVDGSSIYIYGEGWDFGEVANNGRG 602

Query: 1841 INASQSNLAGSGIGSFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARI 1987
            INASQ N+ G+GIGSFNDRIR A+L           GF+TGL LQPNGHDHG EA A  +
Sbjct: 603  INASQFNIHGTGIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGPEAVAEHM 662

Query: 1988 LAISKDHIQVGMAANLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDN 2167
            LA SKDHIQVGMAANL++FVLTNC+G +VKGSE+LT GG+PVAY   P ETINYVSAHDN
Sbjct: 663  LAESKDHIQVGMAANLRDFVLTNCEGKEVKGSEVLTYGGTPVAYTLCPTETINYVSAHDN 722

Query: 2168 ETLFDIISLKTPMEISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSG 2347
            ETLFDI+SLKTPMEISV+E C+INHLATS+IAL+QG+PFFH GDEILRSKSLD DSYNSG
Sbjct: 723  ETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGIPFFHSGDEILRSKSLDRDSYNSG 782

Query: 2348 DWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLR 2527
            DWFNRLDF+Y+SNNWGVGLPPK KNEKSWP+ KPRLA PSFKPQKSHII+A+ENF NLLR
Sbjct: 783  DWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLADPSFKPQKSHIIAAVENFSNLLR 842

Query: 2528 IRYSSILFRLRTANAIQERVRFHNT 2602
            IRYSS LFRLRTANAIQERVRFHNT
Sbjct: 843  IRYSSPLFRLRTANAIQERVRFHNT 867


>ref|XP_019236140.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
            attenuata]
          Length = 957

 Score = 1214 bits (3140), Expect = 0.0
 Identities = 596/835 (71%), Positives = 688/835 (82%), Gaps = 17/835 (2%)
 Frame = +2

Query: 149  SYPRNRYMKQTSYLYLY*PN-NIETLLLSFYSKNLKMTQPQARS-----RDSLSYSRAYW 310
            S+ +NR   Q S L  Y P  N+ T        +  M Q  A S     +DSL YSRA+W
Sbjct: 27   SHLKNRRRLQPSSLTRYRPGQNLGTSRPFTLRCSSTMHQSSAPSSCSQLQDSLFYSRAFW 86

Query: 311  VSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQSLPQNVRDKFPH 490
            VSRSIIAW+V AG G C LY+S++A L VA+  IQG+D   +LER +  LPQ V +KFPH
Sbjct: 87   VSRSIIAWNVGAGDGECYLYASKNAALCVADGEIQGHDVKIKLERTNYGLPQQVVEKFPH 146

Query: 491  IRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDDMFSYTGPLGAIF 670
            IRDY AF+VP TL+  S++K QLAV  F++ G+C   TGLQLPG++D++FSYTGPLGA+F
Sbjct: 147  IRDYTAFEVPATLNCQSLVKCQLAVGIFNSDGKCASATGLQLPGILDELFSYTGPLGAVF 206

Query: 671  SDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGPRSWEGCYYVYEV 850
              E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E NGVWSV G R WEGCYYVYEV
Sbjct: 207  DTEAVSLYLWAPTAQEVRALIYKSPAEADPVEIVQLKELNGVWSVKGIRHWEGCYYVYEV 266

Query: 851  SVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNLADEKPDIADFAD 1030
            SVYHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ WDNL DEKP++  F+D
Sbjct: 267  SVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNLQDEKPNLLSFSD 326

Query: 1031 ISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGLTHIHLLPTFQFG 1210
            IS+YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS AG+TH+HLLPTFQF 
Sbjct: 327  ISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITHVHLLPTFQFA 386

Query: 1211 DVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVLWGVPKGSYATNP 1390
             VEDEK KWK VD+E L S PPDS EQQ  ITAIQNEDGYNWGYNPVLWGVPKGSYA+N 
Sbjct: 387  GVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWGVPKGSYASNA 446

Query: 1391 NGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIVPGYYLRRNADGF 1570
            NGPCRI+EFR MVQALNR+GLRVV DVVY+H++ASG  DD SV DKIVPGYYLRRN DG 
Sbjct: 447  NGPCRIIEFRKMVQALNRIGLRVVSDVVYNHINASGHFDDFSVLDKIVPGYYLRRNVDGG 506

Query: 1571 IENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKSTMMRAKSVLKNL 1750
            IENSTCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH+MK TM++AKS+L +L
Sbjct: 507  IENSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKRTMVKAKSLLNSL 566

Query: 1751 SRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFNDRIRGAVL----- 1915
            S+D++GVDG SI++YGEGWDFGEVAN+GRGINASQ NL GSGIGSFNDRIR A+L     
Sbjct: 567  SKDKNGVDGPSIYIYGEGWDFGEVANSGRGINASQFNLFGSGIGSFNDRIRDALLGGSPF 626

Query: 1916 ------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKEFVLTNCDGHKVK 2077
                  GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAANLK+FVLTNCDG +VK
Sbjct: 627  GHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFVLTNCDGEEVK 686

Query: 2078 GSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVDESCKINHLATSV 2257
            GSE+L     PV YAS PVET+NYVSAHDNETLFDIISLKTP +ISV+E C++NHLATSV
Sbjct: 687  GSEVLLHDRKPVGYASSPVETVNYVSAHDNETLFDIISLKTPKDISVEERCRMNHLATSV 746

Query: 2258 IALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVGLPPKGKNEKSWP 2437
            IALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNNWGVGLPPK KNE +WP
Sbjct: 747  IALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEWNWP 806

Query: 2438 IIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQERVRFHNT 2602
            +IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN+IQERVRFHNT
Sbjct: 807  LIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERVRFHNT 861


>ref|XP_015056539.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Solanum
            pennellii]
          Length = 951

 Score = 1211 bits (3133), Expect = 0.0
 Identities = 578/786 (73%), Positives = 670/786 (85%), Gaps = 11/786 (1%)
 Frame = +2

Query: 275  SRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQ 454
            S+DSL YSRA+WV++SIIAW+V AG G C LY+SR A L V ++GIQG+D   +LER + 
Sbjct: 70   SQDSLFYSRAFWVTKSIIAWNVGAGEGECYLYASRKAGLCVGDDGIQGHDVKIKLERTNY 129

Query: 455  SLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDD 634
             LPQ V +KFPHIRDY AF+VP TLD  S++K QLAV  F++ G+C   TGLQLPG++D+
Sbjct: 130  GLPQQVIEKFPHIRDYAAFEVPATLDFQSLVKCQLAVGIFNSYGKCASATGLQLPGILDE 189

Query: 635  MFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGP 814
            +FSYTGPLG IF+ E VSLYLWAPTAQ V ALIY  P   +P+EIVQL E +GVWS  GP
Sbjct: 190  LFSYTGPLGGIFATEAVSLYLWAPTAQAVEALIYKSPSEADPVEIVQLKELDGVWSAKGP 249

Query: 815  RSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNL 994
            R WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ WDNL
Sbjct: 250  RHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 309

Query: 995  ADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGL 1174
             DEKP++  F+D+SIYELH+RDFSAND TV  E +GGYLAFTS++SAGV HLK+LS+AG+
Sbjct: 310  QDEKPNLLSFSDVSIYELHVRDFSANDPTVPHEFQGGYLAFTSQDSAGVQHLKRLSNAGI 369

Query: 1175 THIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVL 1354
            TH+HLLPT+QF  VEDEK KWK  D+E L S PPDS EQQ  ITAIQ+EDGYNWGYNPVL
Sbjct: 370  THVHLLPTYQFAGVEDEKHKWKYTDIEKLNSFPPDSEEQQALITAIQDEDGYNWGYNPVL 429

Query: 1355 WGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIV 1534
            WGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+HLHASGP  D SV DKIV
Sbjct: 430  WGVPKGSYASNANGPCRIVEFRKMVQALNRIGLRVVLDVVYNHLHASGPFSDFSVLDKIV 489

Query: 1535 PGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKS 1714
            PGY+LRRNADG IENSTCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH+MK 
Sbjct: 490  PGYFLRRNADGAIENSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKR 549

Query: 1715 TMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFND 1894
            TM++AKS+L +LS +E+GVDGSSI++YGEGWDFGEVA NGRGINASQ NL G+GIGSFND
Sbjct: 550  TMLKAKSLLNSLSTEENGVDGSSIYIYGEGWDFGEVAKNGRGINASQFNLFGAGIGSFND 609

Query: 1895 RIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKE 2041
            RIR A+L           GF+TGL  +PNGHD G +A+  R+L +SKDHIQVGMAANLK+
Sbjct: 610  RIRDALLGGSPFGHPLHQGFVTGLYSEPNGHDLGDKANVERMLTVSKDHIQVGMAANLKD 669

Query: 2042 FVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVD 2221
            FVLTNCDG +VKGSE+L     PV YAS PVET+NYVSAHDNETLFD+ISLKTP +ISV+
Sbjct: 670  FVLTNCDGQEVKGSEVLMHDRKPVGYASSPVETVNYVSAHDNETLFDVISLKTPEDISVE 729

Query: 2222 ESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVG 2401
            E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNNWGVG
Sbjct: 730  ERCRMNHLATSVIALSQGIPFFHAGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVG 789

Query: 2402 LPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQE 2581
            LPPK KNE++WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRL+TANAIQE
Sbjct: 790  LPPKEKNERNWPLIKPRLADPSYKPQKSHILAAVENFLNLMQIRYSSPLFRLKTANAIQE 849

Query: 2582 RVRFHN 2599
            RVRFHN
Sbjct: 850  RVRFHN 855


>ref|XP_016436612.1| PREDICTED: pullulanase 1, chloroplastic-like [Nicotiana tabacum]
          Length = 952

 Score = 1210 bits (3130), Expect = 0.0
 Identities = 580/791 (73%), Positives = 673/791 (85%), Gaps = 11/791 (1%)
 Frame = +2

Query: 263  PQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELE 442
            P + S+DSL YSRA+WVSRSIIAW+V AG G C LY+SR+A L VA+  IQG+D   +LE
Sbjct: 66   PSSCSQDSLFYSRAFWVSRSIIAWNVGAGEGECYLYASRNAALCVADGEIQGHDVKIKLE 125

Query: 443  RHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPG 622
            R +  L Q V + FPHIRDY++F+VP  L+  S++K QLAV  F++ G+C   TGLQLPG
Sbjct: 126  RTNYGLSQQVVENFPHIRDYRSFEVPAILNCQSLVKCQLAVGIFNSDGKCASATGLQLPG 185

Query: 623  VIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWS 802
            ++D++FSYTGPLGA+F  E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E NGVWS
Sbjct: 186  ILDELFSYTGPLGAVFDTEAVSLYLWAPTAQEVRALIYKSPSEADPVEIVQLKELNGVWS 245

Query: 803  VNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQK 982
            V GPR WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ 
Sbjct: 246  VKGPRHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEG 305

Query: 983  WDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLS 1162
            WDNL DEKP++  F+DIS+YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS
Sbjct: 306  WDNLQDEKPNLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLS 365

Query: 1163 DAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGY 1342
             AG+TH+HLLPTFQF  VEDEK KWK VD+E L S PPDS EQQ  ITAIQN+DGYNWGY
Sbjct: 366  SAGITHVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNKDGYNWGY 425

Query: 1343 NPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVY 1522
            NPVLWGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+H++ASG  DD SV 
Sbjct: 426  NPVLWGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVLDVVYNHINASGHFDDFSVL 485

Query: 1523 DKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGH 1702
            DKIVPGYYLRRNADG IE+STCVNNTASEHFMV+RLI+DDL  W V+YKIDGFRFDLMGH
Sbjct: 486  DKIVPGYYLRRNADGGIEHSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGH 545

Query: 1703 MMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIG 1882
            +MK TM++AKS+L +LS+D++GVDG SI++YGEGWDFGEVANNGRGINASQ NL GSGIG
Sbjct: 546  IMKRTMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIG 605

Query: 1883 SFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAA 2029
            SFNDRIR A+L           GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAA
Sbjct: 606  SFNDRIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAA 665

Query: 2030 NLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPME 2209
            NLK+FVL+NCDG +VKGSE+L     PV YAS P+ET+NYVSAHDNETLFDIISLKTP +
Sbjct: 666  NLKDFVLSNCDGEEVKGSEVLLHDRKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKD 725

Query: 2210 ISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNN 2389
            ISV+E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNN
Sbjct: 726  ISVEERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNN 785

Query: 2390 WGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTAN 2569
            WG GLPPK KNE +WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN
Sbjct: 786  WGGGLPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLVQIRYSSPLFRLRTAN 845

Query: 2570 AIQERVRFHNT 2602
            +IQERVRFHNT
Sbjct: 846  SIQERVRFHNT 856


>gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 966

 Score = 1209 bits (3129), Expect = 0.0
 Identities = 583/806 (72%), Positives = 670/806 (83%), Gaps = 12/806 (1%)
 Frame = +2

Query: 221  LLLSFYSKNLKMTQPQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVA 400
            L  S  S  +   +  + S+ SL YSRA+WVS SIIAW+V  G GSC L +S++A LS+ 
Sbjct: 63   LQCSASSLYMSAEESTSTSQGSLLYSRAFWVSESIIAWNVYVGNGSCYLLASKTAALSLT 122

Query: 401  ENGIQGYDSNFELERHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDA 580
             +GI G D   +LE     LP+NV++KFPHI+DY+AF VP  LD   +LK QLAVA F++
Sbjct: 123  SDGILGEDVKVKLEEDKHGLPENVKEKFPHIKDYRAFNVPPDLDAKPLLKCQLAVATFNS 182

Query: 581  SGRCIGVTGLQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTAQN-VRALIYNGPYVVE 757
             GRC   TGLQLPG++DD+FSY GPLGA++S ++VSLYLWAPTAQ  V   IY  P    
Sbjct: 183  DGRCSDATGLQLPGILDDLFSYNGPLGALYSKQSVSLYLWAPTAQQEVCVCIYKEPSGGS 242

Query: 758  PLEIVQLIESNGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKR 937
            PLE+VQL E NGVWS  GP+SWEGCYYVYEVSVYHPSTLRIEKC ANDPYARGLS DG+R
Sbjct: 243  PLEVVQLEEFNGVWSTKGPKSWEGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRR 302

Query: 938  TFLVNLDSDNVKPQKWDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAF 1117
            T LVNLDSDN+KP+ WD L DEKPDI  F+DISIYELHIRDFSA+D  V+PE RGGYLAF
Sbjct: 303  TLLVNLDSDNIKPEGWDKLVDEKPDILSFSDISIYELHIRDFSASDQAVHPEFRGGYLAF 362

Query: 1118 TSEESAGVLHLKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQE 1297
            T ++SAG +HLKKLS+AG+TH+HLLP FQF  V+DEKE WKSVD E+L+  P DS +QQ 
Sbjct: 363  TLQDSAGAIHLKKLSNAGITHVHLLPAFQFAGVDDEKENWKSVDPEVLEKFPSDSDKQQA 422

Query: 1298 YITAIQNEDGYNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVY 1477
             ITAIQN+DGYNWGYNPVLWGVPKGSYA+N NG  R +EFR MVQALNR GLRVVLDVVY
Sbjct: 423  LITAIQNDDGYNWGYNPVLWGVPKGSYASNANGTYRAIEFRKMVQALNRFGLRVVLDVVY 482

Query: 1478 SHLHASGPTDDNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWV 1657
            +HLH SGP DDNSV DKIVPGYYLRRN DGFIE+STCVNNTASEHFMV+RLIVDDL++W 
Sbjct: 483  NHLHGSGPVDDNSVLDKIVPGYYLRRNTDGFIEHSTCVNNTASEHFMVERLIVDDLLHWA 542

Query: 1658 VNYKIDGFRFDLMGHMMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGR 1837
            V+YK+DGFRFDLMGH+M+ TM++AK  L +L+++ DGVDGSSI++YGEGWDFGEVANNGR
Sbjct: 543  VDYKVDGFRFDLMGHIMRRTMVKAKDALCSLTKERDGVDGSSIYIYGEGWDFGEVANNGR 602

Query: 1838 GINASQSNLAGSGIGSFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAAR 1984
            GINASQ N+ G+GIGSFNDRIR A+L           GF+TGL LQPNGHDHG EA A  
Sbjct: 603  GINASQFNIHGTGIGSFNDRIRDAILGGSPFGHPLQQGFVTGLLLQPNGHDHGPEAVAEH 662

Query: 1985 ILAISKDHIQVGMAANLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHD 2164
            +LA SKDHIQVGMAANL++FVLTNC+G +VKGSE+LT GG+PVAY   P ETINYVSAHD
Sbjct: 663  MLAESKDHIQVGMAANLRDFVLTNCEGKEVKGSEVLTYGGTPVAYTLCPTETINYVSAHD 722

Query: 2165 NETLFDIISLKTPMEISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNS 2344
            NETLFDI+SLKTPMEISV+E C+INHLATS+IAL+QG+PFFH GDEILRSKSLD DSYNS
Sbjct: 723  NETLFDIVSLKTPMEISVEERCRINHLATSIIALAQGIPFFHSGDEILRSKSLDRDSYNS 782

Query: 2345 GDWFNRLDFSYNSNNWGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLL 2524
            GDWFNRLDF+Y+SNNWGVGLPPK KNEKSWP+ KPRLA PSFKPQKSHII+A+ENF NLL
Sbjct: 783  GDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLADPSFKPQKSHIIAAVENFSNLL 842

Query: 2525 RIRYSSILFRLRTANAIQERVRFHNT 2602
            RIRYSS LFRLRTANAIQERVRFHNT
Sbjct: 843  RIRYSSPLFRLRTANAIQERVRFHNT 868


>gb|PHT29890.1| Pullulanase 1, chloroplastic [Capsicum baccatum]
          Length = 954

 Score = 1209 bits (3127), Expect = 0.0
 Identities = 575/787 (73%), Positives = 672/787 (85%), Gaps = 11/787 (1%)
 Frame = +2

Query: 275  SRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELERHDQ 454
            S+DSL YSRA+WV++SIIAW+V  G   C LY+SR A L V ++GIQG+D   +LER + 
Sbjct: 72   SQDSLFYSRAFWVTKSIIAWNVGGGEDECYLYASRKAALCVDDDGIQGHDVKIKLERTNY 131

Query: 455  SLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPGVIDD 634
             LPQ V +KFPHIRDY AF+VP+TLD  S++K QLAV  F++ G+C   TGLQLPG++D+
Sbjct: 132  GLPQQVIEKFPHIRDYAAFEVPKTLDFQSLVKCQLAVGIFNSDGKCASATGLQLPGILDE 191

Query: 635  MFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWSVNGP 814
            +FSYTGPLGAIF+ E VSLYLWAPTAQ V+ALIY  P  V+P+EIVQL E +GVWS  GP
Sbjct: 192  LFSYTGPLGAIFATEAVSLYLWAPTAQAVQALIYKSPSEVDPVEIVQLKELDGVWSAKGP 251

Query: 815  RSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQKWDNL 994
            R WEGCYYVYEVSVYHPSTLRIEKC++NDPYARGLS DGKRT LVNLDSD+VKP+ WDNL
Sbjct: 252  RHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 311

Query: 995  ADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLSDAGL 1174
              EKP +  F+D+SIYELH+RDFSAND TV  E +GGYLAFT ++SAGV HLKKLS AG+
Sbjct: 312  QHEKPKLLSFSDVSIYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVQHLKKLSSAGV 371

Query: 1175 THIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGYNPVL 1354
            TH+HLLPTFQF  VEDEK+KWK +D+E+L S PPDS EQQ  IT+IQ+EDGYNWGYNPVL
Sbjct: 372  THVHLLPTFQFAGVEDEKDKWKHIDIEILNSFPPDSEEQQALITSIQDEDGYNWGYNPVL 431

Query: 1355 WGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVYDKIV 1534
            WGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+HL+ASG  DD SV DKIV
Sbjct: 432  WGVPKGSYASNANGPCRIVEFRKMVQALNRIGLRVVLDVVYNHLNASGHIDDFSVLDKIV 491

Query: 1535 PGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGHMMKS 1714
            PGY+LRRN DG IE+STCVNNTASEHFMV+RLI+DDL  W V+YK+DGFRFDLMGH+MK 
Sbjct: 492  PGYFLRRNVDGGIEHSTCVNNTASEHFMVERLILDDLKCWAVDYKVDGFRFDLMGHIMKR 551

Query: 1715 TMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIGSFND 1894
            TM++AKS+L +LS + +GVDGSSI++YGEGWDFGEVANNGRGINASQ NL GSGIGSFND
Sbjct: 552  TMLKAKSLLNSLSTENNGVDGSSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIGSFND 611

Query: 1895 RIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAANLKE 2041
            RIR A+L           GF+TGL L+PNGHD G EA+  R+L +S DHIQVGMAANLK+
Sbjct: 612  RIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDEANVKRMLTVSTDHIQVGMAANLKD 671

Query: 2042 FVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPMEISVD 2221
            FVLTNCDG +VKGS++L   G PV YAS P+ET+NYVSAHDNETLFDIISLKTP +ISV+
Sbjct: 672  FVLTNCDGQQVKGSDVLLHDGKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKDISVE 731

Query: 2222 ESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNNWGVG 2401
            E C++NHLATS+IALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNNWGVG
Sbjct: 732  ERCRMNHLATSIIALSQGIPFFHAGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVG 791

Query: 2402 LPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTANAIQE 2581
            LPPK KNE++WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN+IQE
Sbjct: 792  LPPKEKNERNWPLIKPRLADPSYKPQKSHILAAVENFLNLMQIRYSSPLFRLRTANSIQE 851

Query: 2582 RVRFHNT 2602
            RVRFHNT
Sbjct: 852  RVRFHNT 858


>ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vitis vinifera]
 emb|CBI31395.3| unnamed protein product, partial [Vitis vinifera]
          Length = 956

 Score = 1207 bits (3124), Expect = 0.0
 Identities = 575/796 (72%), Positives = 674/796 (84%), Gaps = 11/796 (1%)
 Frame = +2

Query: 248  LKMTQPQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDS 427
            L+++   A+ +DSL YSRAYWVS SIIAW+VD G GSC LYSS+ A LS+ ++GI G+D 
Sbjct: 63   LEVSTSTAQLQDSLLYSRAYWVSESIIAWNVDVGDGSCYLYSSKIAALSIGDSGITGHDM 122

Query: 428  NFELERHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTG 607
              +LE  +  LP NV +KFP ++ YKAFKVP+ +D  S++K QLAVA F   G+C  VTG
Sbjct: 123  TIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTG 182

Query: 608  LQLPGVIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIES 787
            LQLPGV+D++FSY GPLGAIFS E V+LYLWAPTAQ V A IY  P   +PLEIV L E 
Sbjct: 183  LQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEF 242

Query: 788  NGVWSVNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDN 967
            NGVWS +GP+SWEGCYY YEVSVYHPSTL+IEKC ANDPYARGLS D +RT LVNLDSD+
Sbjct: 243  NGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADARRTLLVNLDSDD 302

Query: 968  VKPQKWDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLH 1147
            +KP+ WDNLADEKP +  F+DISIYELHIRDFSA+D TV+P+ RGGYLAFTS++SAG+LH
Sbjct: 303  LKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYLAFTSQDSAGILH 362

Query: 1148 LKKLSDAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDG 1327
            LKKL +AG++H+HLLPTFQF  V+DEK+KWK VD  +L++LPPDS EQQ  I  IQ+EDG
Sbjct: 363  LKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQQAQIMTIQDEDG 422

Query: 1328 YNWGYNPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTD 1507
            YNWGYNPVLWGVPKGSYA+NPN PCR +EFR MVQALNR+G RVVLDVVY+HLH SGP D
Sbjct: 423  YNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFD 482

Query: 1508 DNSVYDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRF 1687
            +NSV DKIVPGYYLRRN+DG IE+S CVNNTASEHFMV+RLI+DDL+ W V+YK+DGFRF
Sbjct: 483  ENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFMVERLILDDLLCWAVDYKVDGFRF 542

Query: 1688 DLMGHMMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLA 1867
            DLMGH+MK TM++AK++L +LS+D DG+DGSSI++YGEGWDFGEVA NGRG+NASQ NL 
Sbjct: 543  DLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLC 602

Query: 1868 GSGIGSFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQ 2014
            G+GIGSFNDRIR A+L           GF+TGL LQPN HDHG E  A R+LA+SKDHIQ
Sbjct: 603  GTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNDHDHGGEEVAERMLAVSKDHIQ 662

Query: 2015 VGMAANLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISL 2194
            VGMAANLK+FVLTNC+G +VKGSE+ T  G+PVAY   P ETINYVSAHDNETLFDI+SL
Sbjct: 663  VGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTVCPTETINYVSAHDNETLFDIVSL 722

Query: 2195 KTPMEISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFS 2374
            KTPM+ISV+E C+INHLATS+IALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFS
Sbjct: 723  KTPMQISVEERCRINHLATSIIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFS 782

Query: 2375 YNSNNWGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFR 2554
            YNSNNWGVGLPPK KNEK+WP+IKPRLA PSFKPQK+HII+A+ENF NLL+IRYSS LFR
Sbjct: 783  YNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKNHIIAAVENFLNLLKIRYSSPLFR 842

Query: 2555 LRTANAIQERVRFHNT 2602
            LRTAN+IQ RVRFHNT
Sbjct: 843  LRTANSIQARVRFHNT 858


>ref|XP_016443817.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X2 [Nicotiana
            tabacum]
          Length = 927

 Score = 1207 bits (3122), Expect = 0.0
 Identities = 580/791 (73%), Positives = 671/791 (84%), Gaps = 11/791 (1%)
 Frame = +2

Query: 263  PQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELE 442
            P + S+DSL YSRA+WVSRSIIAW+V AG G C LY+SR+A L VA+  IQG+D   +LE
Sbjct: 66   PSSCSQDSLFYSRAFWVSRSIIAWNVGAGEGECYLYASRNAALCVADGEIQGHDVKIKLE 125

Query: 443  RHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPG 622
            + +  L Q V +KFPHIRDY AF+VP T++  S++K QLAVA F++ GRC   TGLQLPG
Sbjct: 126  QTNYGLSQQVVEKFPHIRDYTAFEVPATMNCQSLVKCQLAVAIFNSDGRCASATGLQLPG 185

Query: 623  VIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWS 802
            ++D++FSYTGPLGA+F  E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E  GVWS
Sbjct: 186  ILDELFSYTGPLGAVFDTEAVSLYLWAPTAQVVRALIYKSPSETDPVEIVQLKELKGVWS 245

Query: 803  VNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQK 982
              GPR WEGCYYVYEVSVYH STLRIEKC++NDPYARGLS DGKRT LVNL SD+VKP+ 
Sbjct: 246  AKGPRHWEGCYYVYEVSVYHHSTLRIEKCVSNDPYARGLSADGKRTLLVNLVSDDVKPEG 305

Query: 983  WDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLS 1162
            WDNL DEKPD+  F+DIS+YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS
Sbjct: 306  WDNLQDEKPDLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLS 365

Query: 1163 DAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGY 1342
             AG+TH+HLLPTFQF  VEDEK KWK VD+E L S PPDS EQQ  ITAIQNEDGYNWGY
Sbjct: 366  SAGITHVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGY 425

Query: 1343 NPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVY 1522
            NPV+WGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+H++ASG  DD SV 
Sbjct: 426  NPVIWGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVLDVVYNHINASGHFDDFSVL 485

Query: 1523 DKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGH 1702
            DKIVPGYYLRRNADG IE+STCVNNTASEHFMV+RLI+DDL  W V++KIDGFRFDLMGH
Sbjct: 486  DKIVPGYYLRRNADGGIEHSTCVNNTASEHFMVERLILDDLKCWAVHFKIDGFRFDLMGH 545

Query: 1703 MMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIG 1882
            +MK TM++AKS+L +LS+D++GVDG SI++YGEGWDFGEVANNGRGINASQ NL GSGIG
Sbjct: 546  IMKRTMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIG 605

Query: 1883 SFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAA 2029
            SFNDRIR A+L           GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAA
Sbjct: 606  SFNDRIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAA 665

Query: 2030 NLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPME 2209
            NLK+FVLTNCDG +VKGSE+      PV YAS P+ET+NYVSAHDNETLFDIISLKTP +
Sbjct: 666  NLKDFVLTNCDGEEVKGSEVFLHDRKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKD 725

Query: 2210 ISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNN 2389
            ISV+E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNN
Sbjct: 726  ISVEERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNN 785

Query: 2390 WGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTAN 2569
            WGVGLPPK KNE +WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN
Sbjct: 786  WGVGLPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTAN 845

Query: 2570 AIQERVRFHNT 2602
            +IQERVRFHNT
Sbjct: 846  SIQERVRFHNT 856


>ref|XP_016443816.1| PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Nicotiana
            tabacum]
          Length = 952

 Score = 1207 bits (3122), Expect = 0.0
 Identities = 580/791 (73%), Positives = 671/791 (84%), Gaps = 11/791 (1%)
 Frame = +2

Query: 263  PQARSRDSLSYSRAYWVSRSIIAWDVDAGGGSCLLYSSRSATLSVAENGIQGYDSNFELE 442
            P + S+DSL YSRA+WVSRSIIAW+V AG G C LY+SR+A L VA+  IQG+D   +LE
Sbjct: 66   PSSCSQDSLFYSRAFWVSRSIIAWNVGAGEGECYLYASRNAALCVADGEIQGHDVKIKLE 125

Query: 443  RHDQSLPQNVRDKFPHIRDYKAFKVPETLDLNSILKDQLAVAFFDASGRCIGVTGLQLPG 622
            + +  L Q V +KFPHIRDY AF+VP T++  S++K QLAVA F++ GRC   TGLQLPG
Sbjct: 126  QTNYGLSQQVVEKFPHIRDYTAFEVPATMNCQSLVKCQLAVAIFNSDGRCASATGLQLPG 185

Query: 623  VIDDMFSYTGPLGAIFSDENVSLYLWAPTAQNVRALIYNGPYVVEPLEIVQLIESNGVWS 802
            ++D++FSYTGPLGA+F  E VSLYLWAPTAQ VRALIY  P   +P+EIVQL E  GVWS
Sbjct: 186  ILDELFSYTGPLGAVFDTEAVSLYLWAPTAQVVRALIYKSPSETDPVEIVQLKELKGVWS 245

Query: 803  VNGPRSWEGCYYVYEVSVYHPSTLRIEKCIANDPYARGLSGDGKRTFLVNLDSDNVKPQK 982
              GPR WEGCYYVYEVSVYH STLRIEKC++NDPYARGLS DGKRT LVNL SD+VKP+ 
Sbjct: 246  AKGPRHWEGCYYVYEVSVYHHSTLRIEKCVSNDPYARGLSADGKRTLLVNLVSDDVKPEG 305

Query: 983  WDNLADEKPDIADFADISIYELHIRDFSANDSTVYPEARGGYLAFTSEESAGVLHLKKLS 1162
            WDNL DEKPD+  F+DIS+YELH+RDFSAND TV  E +GGYLAFT ++SAGV HLK+LS
Sbjct: 306  WDNLQDEKPDLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLS 365

Query: 1163 DAGLTHIHLLPTFQFGDVEDEKEKWKSVDMEMLKSLPPDSAEQQEYITAIQNEDGYNWGY 1342
             AG+TH+HLLPTFQF  VEDEK KWK VD+E L S PPDS EQQ  ITAIQNEDGYNWGY
Sbjct: 366  SAGITHVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGY 425

Query: 1343 NPVLWGVPKGSYATNPNGPCRIMEFRNMVQALNRLGLRVVLDVVYSHLHASGPTDDNSVY 1522
            NPV+WGVPKGSYA+N NGPCRI+EFR MVQALNR+GLRVVLDVVY+H++ASG  DD SV 
Sbjct: 426  NPVIWGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVLDVVYNHINASGHFDDFSVL 485

Query: 1523 DKIVPGYYLRRNADGFIENSTCVNNTASEHFMVDRLIVDDLINWVVNYKIDGFRFDLMGH 1702
            DKIVPGYYLRRNADG IE+STCVNNTASEHFMV+RLI+DDL  W V++KIDGFRFDLMGH
Sbjct: 486  DKIVPGYYLRRNADGGIEHSTCVNNTASEHFMVERLILDDLKCWAVHFKIDGFRFDLMGH 545

Query: 1703 MMKSTMMRAKSVLKNLSRDEDGVDGSSIFLYGEGWDFGEVANNGRGINASQSNLAGSGIG 1882
            +MK TM++AKS+L +LS+D++GVDG SI++YGEGWDFGEVANNGRGINASQ NL GSGIG
Sbjct: 546  IMKRTMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIG 605

Query: 1883 SFNDRIRGAVL-----------GFLTGLSLQPNGHDHGTEADAARILAISKDHIQVGMAA 2029
            SFNDRIR A+L           GF+TGL L+PNGHD G +A+  R+LA+SKDHIQVGMAA
Sbjct: 606  SFNDRIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAA 665

Query: 2030 NLKEFVLTNCDGHKVKGSEILTCGGSPVAYASQPVETINYVSAHDNETLFDIISLKTPME 2209
            NLK+FVLTNCDG +VKGSE+      PV YAS P+ET+NYVSAHDNETLFDIISLKTP +
Sbjct: 666  NLKDFVLTNCDGEEVKGSEVFLHDRKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKD 725

Query: 2210 ISVDESCKINHLATSVIALSQGVPFFHCGDEILRSKSLDSDSYNSGDWFNRLDFSYNSNN 2389
            ISV+E C++NHLATSVIALSQG+PFFH GDE+LRSKS+D DSYNSGDWFNRLDFSYNSNN
Sbjct: 726  ISVEERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNN 785

Query: 2390 WGVGLPPKGKNEKSWPIIKPRLASPSFKPQKSHIISALENFQNLLRIRYSSILFRLRTAN 2569
            WGVGLPPK KNE +WP+IKPRLA PS+KPQKSHI++A+ENF NL++IRYSS LFRLRTAN
Sbjct: 786  WGVGLPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTAN 845

Query: 2570 AIQERVRFHNT 2602
            +IQERVRFHNT
Sbjct: 846  SIQERVRFHNT 856