BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00007038
         (822 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KVH93994.1| Berberine/berberine-like protein [Cynara carduncu...   442   e-151
ref|XP_021996510.1| berberine bridge enzyme-like 15 [Helianthus ...   441   e-150
ref|XP_021990564.1| berberine bridge enzyme-like 13 [Helianthus ...   441   e-150
gb|OTG13348.1| putative FAD-binding Berberine family protein [He...   438   e-149
ref|XP_023744281.1| berberine bridge enzyme-like 15 [Lactuca sat...   434   e-148
ref|XP_021972790.1| berberine bridge enzyme-like 13 [Helianthus ...   410   e-138
ref|XP_022027161.1| berberine bridge enzyme-like 13 isoform X2 [...   381   e-127
ref|XP_022027160.1| berberine bridge enzyme-like 13 isoform X1 [...   381   e-127
ref|XP_022027162.1| berberine bridge enzyme-like 13 [Helianthus ...   376   e-125
gb|KVI01148.1| Berberine/berberine-like protein [Cynara carduncu...   373   e-124
ref|XP_021639309.1| berberine bridge enzyme-like 15 [Hevea brasi...   369   e-122
emb|CDP12589.1| unnamed protein product [Coffea canephora]            368   e-122
ref|XP_017232252.1| PREDICTED: flavin-dependent oxidoreductase F...   366   e-121
ref|XP_021634412.1| berberine bridge enzyme-like 13 [Manihot esc...   365   e-121
ref|XP_002523164.1| PREDICTED: flavin-dependent oxidoreductase F...   365   e-120
ref|XP_023756900.1| berberine bridge enzyme-like 15 [Lactuca sat...   363   e-120
ref|XP_002523162.1| PREDICTED: flavin-dependent oxidoreductase F...   363   e-120
ref|XP_017229420.1| PREDICTED: flavin-dependent oxidoreductase F...   362   e-120
ref|XP_011096883.1| berberine bridge enzyme-like 15 [Sesamum ind...   362   e-119
ref|XP_022714495.1| berberine bridge enzyme-like 13 [Durio zibet...   361   e-119

>gb|KVH93994.1| Berberine/berberine-like protein [Cynara cardunculus var. scolymus]
          Length = 562

 Score =  442 bits (1138), Expect = e-151
 Identities = 219/273 (80%), Positives = 237/273 (86%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYA 181
           ASLS +  ++PIQD+FHQCLS DTNS++Q                  STAQNLRCIA YA
Sbjct: 15  ASLSSFSSANPIQDTFHQCLSLDTNSISQLPTIFFTPNTTSFTPILNSTAQNLRCIASYA 74

Query: 182 AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLR 361
           AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEG+SYTSVM+PPYVV+DLAKLR
Sbjct: 75  AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGISYTSVMEPPYVVIDLAKLR 134

Query: 362 GIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRK 541
           GIDV+IED SVWV+AGATIGELYYRVAEKSKT+GVPAGLCTSLGVGGHITGGAYGS+MRK
Sbjct: 135 GIDVDIEDGSVWVEAGATIGELYYRVAEKSKTYGVPAGLCTSLGVGGHITGGAYGSMMRK 194

Query: 542 YGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVT 721
           YGL ADNALDAKIIDANGRILDRKAMGEDVFWAIR     SFGV+VSWKLKLVPVP TVT
Sbjct: 195 YGLGADNALDAKIIDANGRILDRKAMGEDVFWAIRGGGGGSFGVIVSWKLKLVPVPETVT 254

Query: 722 TFNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
            FNV RTLE+GATKILY+WQ VA  LD++LFIR
Sbjct: 255 VFNVQRTLEEGATKILYRWQHVADKLDDDLFIR 287


>ref|XP_021996510.1| berberine bridge enzyme-like 15 [Helianthus annuus]
          Length = 563

 Score =  441 bits (1134), Expect = e-150
 Identities = 219/272 (80%), Positives = 232/272 (85%)
 Frame = +2

Query: 5   SLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAA 184
           SLS  C S+P QDSFHQCL  +T+SL Q                  STAQNLRCIAPYAA
Sbjct: 16  SLSPLCSSNPFQDSFHQCLLANTDSLTQLTDIFFTPNTTAFTPLLNSTAQNLRCIAPYAA 75

Query: 185 KPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRG 364
           KPELI TPLHE+HIQTAVICAKKLGIQLRFRSGGHDYEG+SYTSVMD PYVVVDLAKLRG
Sbjct: 76  KPELILTPLHESHIQTAVICAKKLGIQLRFRSGGHDYEGISYTSVMDLPYVVVDLAKLRG 135

Query: 365 IDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKY 544
           IDV++ED SVWVQAGAT+GELYYRVAEKSKTHG+PAGLCTSLGVGGHITGGAYGS+MRKY
Sbjct: 136 IDVDVEDGSVWVQAGATVGELYYRVAEKSKTHGIPAGLCTSLGVGGHITGGAYGSMMRKY 195

Query: 545 GLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTT 724
           GL ADNALDAKIIDANGR+LDRKAMGEDVFWAIR     SFGV+VSWKLKLVPVP TVT 
Sbjct: 196 GLGADNALDAKIIDANGRLLDRKAMGEDVFWAIRGGGGGSFGVIVSWKLKLVPVPETVTV 255

Query: 725 FNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
           FNV +TLEQGAT ILYKWQQVA  LDENLFIR
Sbjct: 256 FNVQKTLEQGATNILYKWQQVAPQLDENLFIR 287


>ref|XP_021990564.1| berberine bridge enzyme-like 13 [Helianthus annuus]
          Length = 564

 Score =  441 bits (1133), Expect = e-150
 Identities = 219/273 (80%), Positives = 233/273 (85%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYA 181
           ASLS +C S+PIQDSFHQCLS +T S+ Q                  STAQNLRCIA YA
Sbjct: 15  ASLSSFCASNPIQDSFHQCLSLNTISITQLSNIFFTPNNTSFTPLLNSTAQNLRCIASYA 74

Query: 182 AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLR 361
            KPELIFTPLHE+HIQ AVICAKKLGIQLR RSGGHDYEG+SYTSVMDPPYVV+DLAKLR
Sbjct: 75  VKPELIFTPLHESHIQAAVICAKKLGIQLRVRSGGHDYEGMSYTSVMDPPYVVIDLAKLR 134

Query: 362 GIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRK 541
           GI+V++ED SVWV+AGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGS+MRK
Sbjct: 135 GINVDVEDGSVWVEAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSMMRK 194

Query: 542 YGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVT 721
           YGL ADNALDAKIIDANGRILDRKAMGEDVFWAIR     SFGV++SWKLKLVPVP TVT
Sbjct: 195 YGLGADNALDAKIIDANGRILDRKAMGEDVFWAIRGGGGGSFGVILSWKLKLVPVPETVT 254

Query: 722 TFNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
            FNV RTLEQGATK LYKWQQVA  LDENLFIR
Sbjct: 255 VFNVVRTLEQGATKTLYKWQQVADKLDENLFIR 287


>gb|OTG13348.1| putative FAD-binding Berberine family protein [Helianthus annuus]
          Length = 579

 Score =  438 bits (1126), Expect = e-149
 Identities = 218/276 (78%), Positives = 235/276 (85%), Gaps = 3/276 (1%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYA 181
           ASLS +C S PIQDSFHQCLST+T+S NQ                  STAQNLRCIA YA
Sbjct: 27  ASLSSFCASTPIQDSFHQCLSTNTDSSNQLSNTFFTPNNTSFTPLLNSTAQNLRCIASYA 86

Query: 182 AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLR 361
           AKPELIFTPL ETHIQ AVICAK+LGIQLR RSGGHDYEG+SYTS+MDPPY+V+DLAKLR
Sbjct: 87  AKPELIFTPLRETHIQAAVICAKRLGIQLRVRSGGHDYEGISYTSLMDPPYIVIDLAKLR 146

Query: 362 GIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRK 541
           GI+V+IED SVWV+AGATIGELYYRVAEKSKT+G+P G+CTSLGVGGHITGGAYGS+MRK
Sbjct: 147 GINVDIEDGSVWVEAGATIGELYYRVAEKSKTYGIPTGICTSLGVGGHITGGAYGSMMRK 206

Query: 542 YGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVT 721
           YGL ADNALDAKI+DANGRILDRKAMGEDVFWAIR     SFGV+VSWKLKLVPVP TVT
Sbjct: 207 YGLGADNALDAKIVDANGRILDRKAMGEDVFWAIRGGGGGSFGVIVSWKLKLVPVPETVT 266

Query: 722 TFNVPRTLEQGATKILYK---WQQVAHTLDENLFIR 820
            FNVPRTLEQGATKILYK   WQQVA  LDENLFIR
Sbjct: 267 VFNVPRTLEQGATKILYKWQQWQQVADKLDENLFIR 302


>ref|XP_023744281.1| berberine bridge enzyme-like 15 [Lactuca sativa]
          Length = 557

 Score =  434 bits (1116), Expect = e-148
 Identities = 214/272 (78%), Positives = 231/272 (84%)
 Frame = +2

Query: 5   SLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAA 184
           SLS +C S+PIQD+FHQCLST+TNSL Q                  STAQNLRCIA YAA
Sbjct: 18  SLSPFCSSNPIQDTFHQCLSTNTNSLTQLPNIFFTPKNTSFTPLLNSTAQNLRCIASYAA 77

Query: 185 KPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRG 364
           KPE+I TPLHE HIQTAVICAKKLGIQLR RSGGHDYEG SYTSVMDPPYVV+DLAKLRG
Sbjct: 78  KPEVILTPLHENHIQTAVICAKKLGIQLRVRSGGHDYEGSSYTSVMDPPYVVLDLAKLRG 137

Query: 365 IDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKY 544
           IDV+IED SVWV+AGAT+GELYYRVAEKSK HGVP+GLCTSLGVGGHI GGAYGS+MRKY
Sbjct: 138 IDVDIEDGSVWVEAGATVGELYYRVAEKSKVHGVPSGLCTSLGVGGHIVGGAYGSMMRKY 197

Query: 545 GLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTT 724
           GL ADNALDAKIIDANG ILDRKAMGEDVFWA+R     SFG+++SWKLKLVPVP TVT 
Sbjct: 198 GLGADNALDAKIIDANGNILDRKAMGEDVFWALRGGGGGSFGIILSWKLKLVPVPETVTV 257

Query: 725 FNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
           FNV RTLEQGATKILYKWQQVA  LD++LFIR
Sbjct: 258 FNVGRTLEQGATKILYKWQQVADKLDDDLFIR 289


>ref|XP_021972790.1| berberine bridge enzyme-like 13 [Helianthus annuus]
          Length = 539

 Score =  410 bits (1054), Expect = e-138
 Identities = 202/272 (74%), Positives = 224/272 (82%)
 Frame = +2

Query: 5   SLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAA 184
           +LS +C S+ IQDSF QCLS+ T  L                    STA NLRCIAPYAA
Sbjct: 16  TLSPFCSSNQIQDSFLQCLSSSTTHLPNIFYTPNNPSFTSILN---STALNLRCIAPYAA 72

Query: 185 KPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRG 364
           KPE IFTPL+E HIQ AV+CAKKLGI LR+RSGGHDYEGLSYTS MDPPYV++DL+KLRG
Sbjct: 73  KPEAIFTPLNENHIQNAVVCAKKLGIHLRYRSGGHDYEGLSYTSAMDPPYVIIDLSKLRG 132

Query: 365 IDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKY 544
           + V++ D SVWV+AGAT+GELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGS+MRKY
Sbjct: 133 VSVDLADGSVWVEAGATVGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSMMRKY 192

Query: 545 GLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTT 724
           GL ADNALD K++DANGRILDRKAMGEDVFWA+R     SFGV+VSWKLKLVPVP TVT 
Sbjct: 193 GLGADNALDVKLVDANGRILDRKAMGEDVFWALRGGGGASFGVIVSWKLKLVPVPETVTV 252

Query: 725 FNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
           FNV RTLEQGATKILYKWQQVA  LD++LFIR
Sbjct: 253 FNVGRTLEQGATKILYKWQQVADKLDDDLFIR 284


>ref|XP_022027161.1| berberine bridge enzyme-like 13 isoform X2 [Helianthus annuus]
          Length = 530

 Score =  381 bits (978), Expect = e-127
 Identities = 187/273 (68%), Positives = 220/273 (80%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYA 181
           +SL+Q+  S+ IQ +FH+CLS     + Q                  STA+NLRCI P  
Sbjct: 14  SSLAQFSSSNQIQHNFHECLS-----IIQTPNTFFTPQSPNYTIILNSTAENLRCITPSN 68

Query: 182 AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLR 361
            KPELIFTPL+E+HIQTAVICA KL IQL+FRSGGHDYEG+SYTS MDPP+V++DL+K+R
Sbjct: 69  PKPELIFTPLNESHIQTAVICANKLKIQLQFRSGGHDYEGISYTSAMDPPFVLIDLSKMR 128

Query: 362 GIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRK 541
            I+V++EDNSVWV+AGAT+GELYYRVAEKSKTHGV  GLCTS+GVGGH TGGAYGS+MRK
Sbjct: 129 AINVDLEDNSVWVEAGATVGELYYRVAEKSKTHGVVGGLCTSVGVGGHFTGGAYGSMMRK 188

Query: 542 YGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVT 721
           YGL  DNALDAKII+A G ILDRK+MGEDVFWAIR     S+GV+VSWKLKLVPVP+ VT
Sbjct: 189 YGLGVDNALDAKIINAKGEILDRKSMGEDVFWAIRGGGGGSYGVIVSWKLKLVPVPSIVT 248

Query: 722 TFNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
            FNVPRTLEQGATKILYKWQ VA  +D++LF+R
Sbjct: 249 VFNVPRTLEQGATKILYKWQYVAPQIDDDLFMR 281


>ref|XP_022027160.1| berberine bridge enzyme-like 13 isoform X1 [Helianthus annuus]
 gb|OTG30052.1| putative berberine/berberine-like, FAD-binding, type 2 [Helianthus
           annuus]
          Length = 553

 Score =  381 bits (978), Expect = e-127
 Identities = 187/273 (68%), Positives = 220/273 (80%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYA 181
           +SL+Q+  S+ IQ +FH+CLS     + Q                  STA+NLRCI P  
Sbjct: 14  SSLAQFSSSNQIQHNFHECLS-----IIQTPNTFFTPQSPNYTIILNSTAENLRCITPSN 68

Query: 182 AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLR 361
            KPELIFTPL+E+HIQTAVICA KL IQL+FRSGGHDYEG+SYTS MDPP+V++DL+K+R
Sbjct: 69  PKPELIFTPLNESHIQTAVICANKLKIQLQFRSGGHDYEGISYTSAMDPPFVLIDLSKMR 128

Query: 362 GIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRK 541
            I+V++EDNSVWV+AGAT+GELYYRVAEKSKTHGV  GLCTS+GVGGH TGGAYGS+MRK
Sbjct: 129 AINVDLEDNSVWVEAGATVGELYYRVAEKSKTHGVVGGLCTSVGVGGHFTGGAYGSMMRK 188

Query: 542 YGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVT 721
           YGL  DNALDAKII+A G ILDRK+MGEDVFWAIR     S+GV+VSWKLKLVPVP+ VT
Sbjct: 189 YGLGVDNALDAKIINAKGEILDRKSMGEDVFWAIRGGGGGSYGVIVSWKLKLVPVPSIVT 248

Query: 722 TFNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
            FNVPRTLEQGATKILYKWQ VA  +D++LF+R
Sbjct: 249 VFNVPRTLEQGATKILYKWQYVAPQIDDDLFMR 281


>ref|XP_022027162.1| berberine bridge enzyme-like 13 [Helianthus annuus]
 gb|OTG30053.1| putative berberine/berberine-like, FAD-binding, type 2 [Helianthus
           annuus]
          Length = 526

 Score =  376 bits (965), Expect = e-125
 Identities = 183/273 (67%), Positives = 218/273 (79%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYA 181
           +SL+ +  S+ IQ +FH+CLS     + Q                  ST +NLRCI P  
Sbjct: 14  SSLAHFSSSNQIQHNFHECLS-----IIQTPNTFFTPQSPNYTIILNSTTENLRCITPSN 68

Query: 182 AKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLR 361
            KPELIFTPL+E+HIQTA+ICA KL IQLRFRSGGHDYEG+SYTS MDPP++++DL+K+R
Sbjct: 69  PKPELIFTPLNESHIQTAIICANKLKIQLRFRSGGHDYEGISYTSAMDPPFLLIDLSKMR 128

Query: 362 GIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRK 541
            I+V+++DNSVWV+AGAT+GELYYRVAEKSKTHGV  GLCTSLGVGGH TGGAYGS+MRK
Sbjct: 129 AINVDLDDNSVWVEAGATVGELYYRVAEKSKTHGVVGGLCTSLGVGGHFTGGAYGSMMRK 188

Query: 542 YGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVT 721
           YGL  DNALDAKII+A G ILDRK++GEDVFWAIR     S+GV+VSWKLKLVPVP+ VT
Sbjct: 189 YGLGVDNALDAKIINARGEILDRKSIGEDVFWAIRGGGGGSYGVIVSWKLKLVPVPSIVT 248

Query: 722 TFNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
            FNVPRTLEQGATKILYKWQ VA  +D++LF+R
Sbjct: 249 VFNVPRTLEQGATKILYKWQYVAPQIDDDLFMR 281


>gb|KVI01148.1| Berberine/berberine-like protein [Cynara cardunculus var. scolymus]
          Length = 529

 Score =  373 bits (957), Expect = e-124
 Identities = 186/262 (70%), Positives = 210/262 (80%)
 Frame = +2

Query: 35  IQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPELIFTPLH 214
           IQ +F QCLST      Q                  STAQNLRCI P   KP  IFTP +
Sbjct: 26  IQHTFPQCLSTI-----QIPNSFFTPTSPNFTLILNSTAQNLRCITPSTPKPLAIFTPFN 80

Query: 215 ETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVNIEDNSV 394
           +THIQTAVICAKKLGI LRFRSGGHDYEG+SYTS ++ P++V+DL+KLR I+V+++DNSV
Sbjct: 81  QTHIQTAVICAKKLGIHLRFRSGGHDYEGISYTSAINLPFIVIDLSKLRAINVHLDDNSV 140

Query: 395 WVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAADNALDA 574
           WV+AGAT+GELYYRVAEKSKTHG+ AGLCTSLGVGGHITGGAYGS+MRKYGL  DNALDA
Sbjct: 141 WVEAGATVGELYYRVAEKSKTHGIAAGLCTSLGVGGHITGGAYGSMMRKYGLGVDNALDA 200

Query: 575 KIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVPRTLEQG 754
           KII+ANG ILDRK+MGEDVFWAI      S+GV+VSWKLKLVPVPATVT FNVPRTLEQG
Sbjct: 201 KIINANGEILDRKSMGEDVFWAISGGGGGSYGVIVSWKLKLVPVPATVTVFNVPRTLEQG 260

Query: 755 ATKILYKWQQVAHTLDENLFIR 820
           ATKIL KWQQVA   D++LFIR
Sbjct: 261 ATKILLKWQQVAPKFDDDLFIR 282


>ref|XP_021639309.1| berberine bridge enzyme-like 15 [Hevea brasiliensis]
          Length = 545

 Score =  369 bits (948), Expect = e-122
 Identities = 179/268 (66%), Positives = 209/268 (77%)
 Frame = +2

Query: 17  YCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPEL 196
           +  S PI DSF QCL  ++  +                    S+AQNLR + P   KPE 
Sbjct: 21  FSLSFPIGDSFLQCLIVNSEIVIPFSTAFYSPDNSSFSSFLQSSAQNLRYLLPSVPKPEF 80

Query: 197 IFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVN 376
           IFTPLHETHIQ AVIC+K+LGI LR RSGGHDYEGLSY S ++ P++VVDL+KLR + V+
Sbjct: 81  IFTPLHETHIQAAVICSKQLGIHLRVRSGGHDYEGLSYASEIEAPFIVVDLSKLRSVTVD 140

Query: 377 IEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAA 556
           IEDNS WVQAGATIGE YYR+AEKSKTHG PAGLC+SLGVGGHITGGAYGS+MRKYGL A
Sbjct: 141 IEDNSAWVQAGATIGEAYYRIAEKSKTHGFPAGLCSSLGVGGHITGGAYGSMMRKYGLGA 200

Query: 557 DNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVP 736
           DN +DA+IID NGR+LDR+AMGED+FWAIR     SFG++VSWKL LVPVPATVT F V 
Sbjct: 201 DNVIDARIIDVNGRVLDRQAMGEDLFWAIRGGGGASFGIIVSWKLNLVPVPATVTVFTVT 260

Query: 737 RTLEQGATKILYKWQQVAHTLDENLFIR 820
           +TLE+GATK+LY+WQQVAH LDE+LFIR
Sbjct: 261 KTLEEGATKLLYRWQQVAHKLDEDLFIR 288


>emb|CDP12589.1| unnamed protein product [Coffea canephora]
          Length = 550

 Score =  368 bits (944), Expect = e-122
 Identities = 174/272 (63%), Positives = 213/272 (78%)
 Frame = +2

Query: 5   SLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAA 184
           SLS    S  +Q++F QC++ ++                       STAQNLRC+ P  A
Sbjct: 24  SLSSIAVSQTVQENFFQCINQNSELYVPFSSAFVSPNNDSFSSVLQSTAQNLRCLEPSVA 83

Query: 185 KPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRG 364
           KP+LIFTPL E+H+Q AVICAK++GIQ+R RSGGHDYEGLSY S +D P+++VDL KLR 
Sbjct: 84  KPQLIFTPLIESHVQAAVICAKEVGIQIRMRSGGHDYEGLSYISDLDSPFIIVDLGKLRA 143

Query: 365 IDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKY 544
           I V+I+DNS WVQAGATIGE+YYR+ +KSKTHG PAGLCTSLG+GGHITGGAYG++MRK+
Sbjct: 144 ISVDIDDNSAWVQAGATIGEVYYRIFQKSKTHGFPAGLCTSLGIGGHITGGAYGTMMRKF 203

Query: 545 GLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTT 724
           GL ADN LDA+I+DA GRILDR++MGED+FWAIR     SFG+++SWK+KLVPVP+ VT 
Sbjct: 204 GLGADNVLDARIVDAQGRILDRQSMGEDLFWAIRGGGGASFGIILSWKIKLVPVPSIVTV 263

Query: 725 FNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
           F VP+TLEQGATKILYKWQQVA  LDE+LFIR
Sbjct: 264 FTVPKTLEQGATKILYKWQQVADKLDEDLFIR 295


>ref|XP_017232252.1| PREDICTED: flavin-dependent oxidoreductase FOX2-like [Daucus carota
           subsp. sativus]
 gb|KZN04824.1| hypothetical protein DCAR_005661 [Daucus carota subsp. sativus]
          Length = 531

 Score =  366 bits (939), Expect = e-121
 Identities = 180/267 (67%), Positives = 210/267 (78%), Gaps = 2/267 (0%)
 Frame = +2

Query: 26  SDPIQDSFHQCLSTDTN-SLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPELIF 202
           S+PIQDSF+QCL  ++  S                     STAQNLRC+ P   KPELIF
Sbjct: 23  SNPIQDSFYQCLGLNSKASPIPFSSCFYTPNITSFSSVLQSTAQNLRCLVPTVPKPELIF 82

Query: 203 TPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVNIE 382
           TP+HETH+Q AV+C KKLGIQLR RSGGHDYEG SYTS M+PP+VVVDL+KLRGI V+IE
Sbjct: 83  TPVHETHVQAAVVCVKKLGIQLRIRSGGHDYEGQSYTSEMEPPFVVVDLSKLRGISVDIE 142

Query: 383 DNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAADN 562
           + + WVQAGATIGE+YYR+AEKSK H  PAGLCTSLGVGGHITGGAYG +MRK+GL ADN
Sbjct: 143 NGTAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVGGHITGGAYGPMMRKFGLGADN 202

Query: 563 ALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVPRT 742
            +DAKIIDANG ILDRK+MGED FWAIR     SFGV++SWK+KLV +P++VT F VP+T
Sbjct: 203 VVDAKIIDANGTILDRKSMGEDYFWAIRGGGGGSFGVILSWKIKLVQIPSSVTVFTVPKT 262

Query: 743 LEQGATKILYKWQQV-AHTLDENLFIR 820
           LE+ ATKILYKWQQV A  LDE+LF+R
Sbjct: 263 LEENATKILYKWQQVAADKLDEDLFLR 289


>ref|XP_021634412.1| berberine bridge enzyme-like 13 [Manihot esculenta]
 gb|OAY31593.1| hypothetical protein MANES_14G125100 [Manihot esculenta]
          Length = 546

 Score =  365 bits (936), Expect = e-121
 Identities = 176/262 (67%), Positives = 208/262 (79%)
 Frame = +2

Query: 35  IQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPELIFTPLH 214
           I+DSF QCL+ +T ++                    S+AQNLR + P   KPE IFTPLH
Sbjct: 27  IRDSFLQCLNVNTETVIPYSTAFFTPDNSSFSSILQSSAQNLRYLLPSVPKPEFIFTPLH 86

Query: 215 ETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVNIEDNSV 394
           ETHIQ AVIC+K+LGI LR RSGGHDYEGLSYTS ++ P+++VDL+KLR + V+IEDNS 
Sbjct: 87  ETHIQAAVICSKQLGIHLRVRSGGHDYEGLSYTSEIETPFIIVDLSKLRSVTVDIEDNSA 146

Query: 395 WVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAADNALDA 574
           WVQAGATIGE YYR+AEKSK HG PAGLC+SLGVGGHITGGAYGS+MRKYGL ADN +DA
Sbjct: 147 WVQAGATIGEAYYRIAEKSKIHGFPAGLCSSLGVGGHITGGAYGSMMRKYGLGADNVIDA 206

Query: 575 KIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVPRTLEQG 754
           +IID NGR+L+R+AMGED+FWAIR     SFG++VSWKLKLVPVPATVT F V +TLEQ 
Sbjct: 207 RIIDVNGRVLNRQAMGEDLFWAIRGGGGASFGIIVSWKLKLVPVPATVTVFTVTKTLEQN 266

Query: 755 ATKILYKWQQVAHTLDENLFIR 820
           AT+ILY+WQQVA  LDE+LFIR
Sbjct: 267 ATEILYRWQQVADKLDEDLFIR 288


>ref|XP_002523164.1| PREDICTED: flavin-dependent oxidoreductase FOX2 [Ricinus communis]
 gb|EEF39195.1| Reticuline oxidase precursor, putative [Ricinus communis]
          Length = 548

 Score =  365 bits (936), Expect = e-120
 Identities = 175/268 (65%), Positives = 209/268 (77%)
 Frame = +2

Query: 17  YCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPEL 196
           + YS PI DSF QCL  ++  L                    S+AQNLR + P   KPE 
Sbjct: 23  FAYSLPIFDSFIQCLKVNSEILIPFSTSFYTHDNSSFSSVLQSSAQNLRYLLPSVPKPEF 82

Query: 197 IFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVN 376
           IFTPLHETH+Q AVIC+K+LGI LR RSGGHDYEGLSY S ++ P++VVDL+KLR + V+
Sbjct: 83  IFTPLHETHVQAAVICSKQLGIHLRVRSGGHDYEGLSYASEIESPFIVVDLSKLRYVSVD 142

Query: 377 IEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAA 556
           I+DNS WVQAGAT+GE YYR++EKS+THG PAGLCTSLG+GGHITGGAYGS+MRKYGL A
Sbjct: 143 IDDNSAWVQAGATVGEAYYRISEKSRTHGFPAGLCTSLGIGGHITGGAYGSMMRKYGLGA 202

Query: 557 DNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVP 736
           DN +DA+IIDANGR+LDR+AMGED+FWAIR     SFG++++WKLKLVPVPA VT F V 
Sbjct: 203 DNVIDARIIDANGRVLDRQAMGEDLFWAIRGGGGASFGIILAWKLKLVPVPAIVTVFTVT 262

Query: 737 RTLEQGATKILYKWQQVAHTLDENLFIR 820
           +TLEQ ATKILY+WQQVA  LDE+LFIR
Sbjct: 263 KTLEQDATKILYRWQQVADKLDEDLFIR 290


>ref|XP_023756900.1| berberine bridge enzyme-like 15 [Lactuca sativa]
 gb|PLY98903.1| hypothetical protein LSAT_7X34661 [Lactuca sativa]
          Length = 532

 Score =  363 bits (932), Expect = e-120
 Identities = 173/272 (63%), Positives = 214/272 (78%)
 Frame = +2

Query: 5   SLSQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAA 184
           S + + +S  IQ +F QCLST      Q                  STAQNLRC+ P   
Sbjct: 17  SFAHFSFSTQIQQTFDQCLSTI-----QVPDTFFTPNNPNFTTLLNSTAQNLRCLTPSYP 71

Query: 185 KPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRG 364
           KP+ IFTPL+E  +QTAVICAKKLGI LRFRSGGHDYEG+S+TS MDPP+V++D++K+R 
Sbjct: 72  KPQAIFTPLNEYDVQTAVICAKKLGIHLRFRSGGHDYEGISFTSAMDPPFVMIDMSKMRD 131

Query: 365 IDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKY 544
           ++V+++DNSVWV+AGAT+GELYYRVAEKS THG+ AGLCTSLGVGGHITGGAYGS+MRKY
Sbjct: 132 VNVDLDDNSVWVEAGATVGELYYRVAEKSSTHGIAAGLCTSLGVGGHITGGAYGSMMRKY 191

Query: 545 GLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTT 724
           GL  DNA+DAKI++A+G+I+DR +MGEDVFWAI      S+GV++SWKLKLVPVPA VT 
Sbjct: 192 GLGVDNAIDAKIVNADGKIMDRVSMGEDVFWAISGGGGGSYGVILSWKLKLVPVPAIVTV 251

Query: 725 FNVPRTLEQGATKILYKWQQVAHTLDENLFIR 820
           FNV +TLEQGATKILY+WQQ+A   D++LF+R
Sbjct: 252 FNVQKTLEQGATKILYRWQQLASNFDDDLFVR 283


>ref|XP_002523162.1| PREDICTED: flavin-dependent oxidoreductase FOX2 [Ricinus communis]
 gb|EEF39193.1| Reticuline oxidase precursor, putative [Ricinus communis]
          Length = 548

 Score =  363 bits (932), Expect = e-120
 Identities = 174/268 (64%), Positives = 210/268 (78%)
 Frame = +2

Query: 17  YCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPEL 196
           + YS  I DSF QCL  ++  L                    S+AQNLR ++P   KPE 
Sbjct: 23  FTYSFRIFDSFIQCLKVNSEILIPFSTSLYTPDNSSFSSVLQSSAQNLRYLSPSVPKPEF 82

Query: 197 IFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVN 376
           IFTPLHETH+Q AVIC+K+LGI LR RSGGHDYEGLSY S ++ P++VVDL+KLR + V+
Sbjct: 83  IFTPLHETHVQAAVICSKQLGIHLRVRSGGHDYEGLSYASEIESPFIVVDLSKLRYVSVD 142

Query: 377 IEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAA 556
           I+DNS WVQAGAT+GE YYR++EKS+THG PAGLCTSLG+GGHITGGAYGS+MRKYGL A
Sbjct: 143 IDDNSAWVQAGATVGEAYYRISEKSRTHGFPAGLCTSLGIGGHITGGAYGSMMRKYGLGA 202

Query: 557 DNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVP 736
           DN +DA+IIDANG++LDR+AMGED+FWAIR     SFG++++WKLKLVPVPATVT F V 
Sbjct: 203 DNVIDARIIDANGKVLDRQAMGEDLFWAIRGGGGASFGIILAWKLKLVPVPATVTVFTVT 262

Query: 737 RTLEQGATKILYKWQQVAHTLDENLFIR 820
           +TLEQ ATKILY+WQQVA  LDE+LFIR
Sbjct: 263 KTLEQDATKILYRWQQVADKLDEDLFIR 290


>ref|XP_017229420.1| PREDICTED: flavin-dependent oxidoreductase FOX2-like [Daucus carota
           subsp. sativus]
 gb|KZN08295.1| hypothetical protein DCAR_000841 [Daucus carota subsp. sativus]
          Length = 540

 Score =  362 bits (929), Expect = e-120
 Identities = 177/275 (64%), Positives = 213/275 (77%), Gaps = 2/275 (0%)
 Frame = +2

Query: 2   ASLSQYCYSDPIQDSFHQCLSTDTN-SLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPY 178
           ++L  +  S+ IQDSF+ CL+ ++  S                     S+AQNLRC+AP 
Sbjct: 15  STLVLHVTSNHIQDSFYHCLALNSKASPIPFSSAFFTPNISSFSTLLQSSAQNLRCLAPS 74

Query: 179 AAKPELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKL 358
             KP+LIFTPLHE+HIQ AV+CAK LGIQ+R RSGGHDYEGLSY S M+PP+VVVDL++L
Sbjct: 75  VPKPQLIFTPLHESHIQAAVVCAKDLGIQIRIRSGGHDYEGLSYVSEMEPPFVVVDLSRL 134

Query: 359 RGIDVNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMR 538
           R + V+IEDN+ WVQAGATIGE+YYR+AEKSK H  PAGLCTSLGVGGHITGGAYG +MR
Sbjct: 135 RSVSVDIEDNTAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVGGHITGGAYGPMMR 194

Query: 539 KYGLAADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATV 718
           KYGL ADN +DAK+IDANG+ILDRKAMGED FWA+R     SFG++VSWK+KLVP+P  V
Sbjct: 195 KYGLGADNVVDAKVIDANGKILDRKAMGEDYFWALRGGGGGSFGIIVSWKIKLVPIPENV 254

Query: 719 TTFNVPRTLEQGATKILYKWQQV-AHTLDENLFIR 820
           T F VP+TLEQ  TKIL++WQQV A  LDE+LFIR
Sbjct: 255 TVFTVPKTLEQNGTKILHRWQQVAADKLDEDLFIR 289


>ref|XP_011096883.1| berberine bridge enzyme-like 15 [Sesamum indicum]
          Length = 542

 Score =  362 bits (928), Expect = e-119
 Identities = 168/265 (63%), Positives = 210/265 (79%)
 Frame = +2

Query: 26  SDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKPELIFT 205
           S P+Q+ F+QC++ ++                       S+AQNLRC+ P   KP+LIFT
Sbjct: 24  SQPVQERFYQCITLNSELSIPFSEAFSAPENASFSTILESSAQNLRCLVPSVPKPQLIFT 83

Query: 206 PLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGIDVNIED 385
           P  E H+Q AVICAK+LGIQLR RSGGHDYEGLSY S MD P++V+DL+KLR + V+++D
Sbjct: 84  PYIENHVQAAVICAKELGIQLRVRSGGHDYEGLSYRSEMDEPFIVLDLSKLRSVRVDMQD 143

Query: 386 NSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGLAADNA 565
           NS W QAGATIGELYYR+++KSKTHG PAGLC+SLG+GGHITGGAYG +MRKYGL ADN 
Sbjct: 144 NSAWAQAGATIGELYYRISQKSKTHGFPAGLCSSLGIGGHITGGAYGPMMRKYGLGADNV 203

Query: 566 LDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFNVPRTL 745
           +DA+I+DA+GRILDR++MGED+FWAIR     SFG++++WK++LVPVPATVT F VP+TL
Sbjct: 204 IDARIVDASGRILDRESMGEDLFWAIRGGGGASFGIILAWKVRLVPVPATVTVFTVPKTL 263

Query: 746 EQGATKILYKWQQVAHTLDENLFIR 820
           EQGATKILYKWQQVA  +DE+LFIR
Sbjct: 264 EQGATKILYKWQQVADKIDEDLFIR 288


>ref|XP_022714495.1| berberine bridge enzyme-like 13 [Durio zibethinus]
          Length = 532

 Score =  361 bits (926), Expect = e-119
 Identities = 172/270 (63%), Positives = 210/270 (77%)
 Frame = +2

Query: 11  SQYCYSDPIQDSFHQCLSTDTNSLNQXXXXXXXXXXXXXXXXXXSTAQNLRCIAPYAAKP 190
           + +  S  IQ+ F QCL  ++  +                    STAQNLRC+ P   KP
Sbjct: 18  ASWATSTSIQEDFIQCLDDNSEHVIPIFTVCAQTNSSFTSILN-STAQNLRCLMPSVPKP 76

Query: 191 ELIFTPLHETHIQTAVICAKKLGIQLRFRSGGHDYEGLSYTSVMDPPYVVVDLAKLRGID 370
           E IFTPL+E+H+Q AVICAKKLGI LRFRSGGHDYEGLSY S ++ P++++DLA+LR I+
Sbjct: 77  EFIFTPLNESHVQAAVICAKKLGIHLRFRSGGHDYEGLSYASQIETPFIMLDLARLRAIN 136

Query: 371 VNIEDNSVWVQAGATIGELYYRVAEKSKTHGVPAGLCTSLGVGGHITGGAYGSLMRKYGL 550
           V+I+DNS WVQAGAT+GE+YYR++E SKTHG PAGLC+SLG+GGHITGGAYGS+MRKYGL
Sbjct: 137 VDIDDNSAWVQAGATVGEVYYRISENSKTHGFPAGLCSSLGIGGHITGGAYGSMMRKYGL 196

Query: 551 AADNALDAKIIDANGRILDRKAMGEDVFWAIRXXXXXSFGVLVSWKLKLVPVPATVTTFN 730
            ADN LDA+I+D NG+ILDR AMGED FWAIR     SFG++++WK+KLVPVP TVT F 
Sbjct: 197 GADNVLDARIVDVNGKILDRAAMGEDFFWAIRGGGGASFGIILAWKIKLVPVPETVTVFT 256

Query: 731 VPRTLEQGATKILYKWQQVAHTLDENLFIR 820
           VP+TLEQGATKILY+WQQVA  LDE+LFIR
Sbjct: 257 VPKTLEQGATKILYRWQQVADKLDEDLFIR 286