BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Chrysanthemum21_contig00004670
         (1738 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_022007572.1| E3 ubiquitin-protein ligase RFWD3-like [Heli...   112   1e-24
ref|XP_022000867.1| putative E3 ubiquitin-protein ligase RING1a ...   104   1e-20
gb|PLY64624.1| hypothetical protein LSAT_6X24500 [Lactuca sativa]      98   1e-19
gb|KVH90417.1| Zinc finger, RING/FYVE/PHD-type [Cynara carduncul...   100   5e-19
ref|XP_023746008.1| E3 ubiquitin-protein ligase RFWD3-like [Lact...    98   5e-19
ref|XP_023746023.1| uncharacterized protein LOC111894169 [Lactuc...    93   4e-18
ref|XP_023745920.1| E3 ubiquitin-protein ligase RFWD3-like [Lact...    95   2e-17
gb|PLY75050.1| hypothetical protein LSAT_2X30721 [Lactuca sativa]      96   5e-17
ref|XP_023732273.1| E3 ubiquitin-protein ligase RFWD3-like [Lact...    96   5e-17
gb|PLY64540.1| hypothetical protein LSAT_6X24480 [Lactuca sativa]      92   8e-17
ref|XP_022009012.1| E3 ubiquitin-protein ligase RFWD3 isoform X2...    95   1e-16
ref|XP_020212865.1| E3 ubiquitin-protein ligase RFWD3-like isofo...    91   2e-16
ref|XP_004494071.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...    94   2e-16
ref|XP_022009011.1| E3 ubiquitin-protein ligase RFWD3 isoform X1...    94   2e-16
gb|PNX94327.1| E3 ubiquitin-protein ligase rfwd3-like protein [T...    93   2e-16
gb|OVA06944.1| zinc finger protein [Macleaya cordata]                  89   3e-16
ref|XP_010543439.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...    93   4e-16
ref|XP_023895811.1| E3 ubiquitin-protein ligase RFWD3 isoform X2...    92   6e-16
ref|XP_023895810.1| E3 ubiquitin-protein ligase RFWD3 isoform X1...    92   6e-16
ref|XP_018470955.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...    92   7e-16

>ref|XP_022007572.1| E3 ubiquitin-protein ligase RFWD3-like [Helianthus annuus]
 gb|OTG34971.1| putative zinc finger, RING/FYVE/PHD-type [Helianthus annuus]
          Length = 222

 Score =  112 bits (281), Expect = 1e-24
 Identities = 55/141 (39%), Positives = 87/141 (61%), Gaps = 5/141 (3%)
 Frame = -1

Query: 1660 DDDIGNKC---QYDDCSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQ 1490
            DD+  N+    ++D CSIC +     G HR+CCLPCGH+YG SCI+KWL+     GKCP+
Sbjct: 79   DDEEKNETKRDEFDVCSICLESWTSGGDHRICCLPCGHIYGMSCIRKWLVWCQSDGKCPK 138

Query: 1489 CNFICAYKDVVLLHASTLCIHAHQK--ATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYT 1316
            C  +C  KD+ +L+A+ LC+ A +K    S   FP+T +GF EF+++ +   +D  K+  
Sbjct: 139  CKSLCTLKDIRVLYATRLCV-ADEKPHEASTINFPYTREGFNEFQQYVF-SCMDELKQRA 196

Query: 1315 DDYMRMVDVVEQQRDLAKQQA 1253
            +D+   + +++Q  D  + QA
Sbjct: 197  EDFDSRLHILQQICDELRAQA 217



 Score = 84.0 bits (206), Expect = 1e-14
 Identities = 40/91 (43%), Positives = 58/91 (63%), Gaps = 4/91 (4%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASR---ASAHQKTSST 737
           +CCLPCGH+YG SCI+KWL    S GKCP+C + C  KD+ +LYA+R   A      +ST
Sbjct: 109 ICCLPCGHIYGMSCIRKWLVWCQSDGKCPKCKSLCTLKDIRVLYATRLCVADEKPHEAST 168

Query: 736 RHFSFTKQGIREFREHERLRRVDTEEMHAED 644
            +F +T++G  EF+++     +D  +  AED
Sbjct: 169 INFPYTREGFNEFQQYV-FSCMDELKQRAED 198


>ref|XP_022000867.1| putative E3 ubiquitin-protein ligase RING1a [Helianthus annuus]
 gb|OTG01324.1| putative zinc finger, RING/FYVE/PHD-type [Helianthus annuus]
          Length = 373

 Score =  104 bits (260), Expect = 1e-20
 Identities = 52/121 (42%), Positives = 69/121 (57%), Gaps = 20/121 (16%)
 Frame = -1

Query: 1732 EMTSPRISCWLGADGKTYQGVMVVD--------------------DDIGNKCQYDDCSIC 1613
            +MTS R+S  +  +G+  QG MV D                    +  G +     C IC
Sbjct: 195  QMTSSRVSSEI-FEGRVLQGGMVSDVGVMEDSDGSESCEGKNGKQEKCGGEFHGSVCPIC 253

Query: 1612 YQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHASTLC 1433
            ++     G+H++CCLPCGH+YG SCIKKWL QS  SGKCPQCN +C  KDV +L+AS LC
Sbjct: 254  FEAWTSGGEHQICCLPCGHIYGLSCIKKWLQQSRGSGKCPQCNNVCTLKDVRVLYASRLC 313

Query: 1432 I 1430
            +
Sbjct: 314  V 314



 Score = 82.4 bits (202), Expect = 4e-13
 Identities = 35/47 (74%), Positives = 38/47 (80%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTSSG-GKCPQCNTSCAYKDVILLYASR 767
           +CCLPCGH+YG SCIKKWLQ S G GKCPQCN  C  KDV +LYASR
Sbjct: 265 ICCLPCGHIYGLSCIKKWLQQSRGSGKCPQCNNVCTLKDVRVLYASR 311


>gb|PLY64624.1| hypothetical protein LSAT_6X24500 [Lactuca sativa]
          Length = 203

 Score = 98.2 bits (243), Expect = 1e-19
 Identities = 50/140 (35%), Positives = 73/140 (52%), Gaps = 23/140 (16%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC+Q     G+H++ CLPCGH+YGFSCIK W+ QS  SGKCPQC  +C  KDV +L+A
Sbjct: 58   CPICFQAWTSGGEHQISCLPCGHIYGFSCIKTWIQQSPSSGKCPQCKKLCTLKDVRVLYA 117

Query: 1444 STLCI-----------------HAHQKAT------SARYFPFTEQGFIEFKEHEWLRHLD 1334
            S LC+                 +  QK          R   + +   IE K  E    +D
Sbjct: 118  SRLCVVDEKLQKRVYSLEAECAYLKQKTVYEDMREEMRKHMYAKAAEIEAKLRETREEMD 177

Query: 1333 AQKRYTDDYMRMVDVVEQQR 1274
            A++++TD     ++++  Q+
Sbjct: 178  AKQKHTDARFEAMEMMFNQK 197



 Score = 76.3 bits (186), Expect = 4e-12
 Identities = 38/89 (42%), Positives = 53/89 (59%), Gaps = 3/89 (3%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTS-SGGKCPQCNTSCAYKDVILLYASRASAHQKTSSTRHF 728
           + CLPCGH+YGFSCIK W+Q S S GKCPQC   C  KDV +LYASR     +    R +
Sbjct: 73  ISCLPCGHIYGFSCIKTWIQQSPSSGKCPQCKKLCTLKDVRVLYASRLCVVDEKLQKRVY 132

Query: 727 SFTKQ--GIREFREHERLRRVDTEEMHAE 647
           S   +   +++   +E +R    + M+A+
Sbjct: 133 SLEAECAYLKQKTVYEDMREEMRKHMYAK 161


>gb|KVH90417.1| Zinc finger, RING/FYVE/PHD-type [Cynara cardunculus var. scolymus]
          Length = 357

 Score = 99.8 bits (247), Expect = 5e-19
 Identities = 57/160 (35%), Positives = 84/160 (52%), Gaps = 9/160 (5%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC++     G H++CCLPCGH+YG SCIKKWL Q   SGKCPQCN +C  KDV +L+A
Sbjct: 198  CPICFEAWTSGGDHQICCLPCGHIYGSSCIKKWLQQRRSSGKCPQCNNLCTLKDVRVLYA 257

Query: 1444 STLCIHAHQKATSARYF----PFTEQGFIEFKEHEWLR-----HLDAQKRYTDDYMRMVD 1292
            + LC+   +     R       + EQ   ++KE +  R     +L+  K +  +++ +  
Sbjct: 258  TRLCVADEELQKRVRSLEARCSYLEQK-DKYKEDDGWRGKFETYLEEFKAHRKEFVELRQ 316

Query: 1291 VVEQQRDLAKQQAAMKPQWDDLVARSVEMNQRAEKLGQRF 1172
             VE  R   + Q     Q  ++ A+ V   Q  E + Q F
Sbjct: 317  EVEAFRQEVEAQLVALRQ--EVEAQLVAFRQEHEAVQQEF 354



 Score = 78.2 bits (191), Expect = 8e-12
 Identities = 33/47 (70%), Positives = 37/47 (78%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASR 767
           +CCLPCGH+YG SCIKKWL Q  S GKCPQCN  C  KDV +LYA+R
Sbjct: 213 ICCLPCGHIYGSSCIKKWLQQRRSSGKCPQCNNLCTLKDVRVLYATR 259


>ref|XP_023746008.1| E3 ubiquitin-protein ligase RFWD3-like [Lactuca sativa]
          Length = 285

 Score = 98.2 bits (243), Expect = 5e-19
 Identities = 50/140 (35%), Positives = 73/140 (52%), Gaps = 23/140 (16%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC+Q     G+H++ CLPCGH+YGFSCIK W+ QS  SGKCPQC  +C  KDV +L+A
Sbjct: 140  CPICFQAWTSGGEHQISCLPCGHIYGFSCIKTWIQQSPSSGKCPQCKKLCTLKDVRVLYA 199

Query: 1444 STLCI-----------------HAHQKAT------SARYFPFTEQGFIEFKEHEWLRHLD 1334
            S LC+                 +  QK          R   + +   IE K  E    +D
Sbjct: 200  SRLCVVDEKLQKRVYSLEAECAYLKQKTVYEDMREEMRKHMYAKAAEIEAKLRETREEMD 259

Query: 1333 AQKRYTDDYMRMVDVVEQQR 1274
            A++++TD     ++++  Q+
Sbjct: 260  AKQKHTDARFEAMEMMFNQK 279



 Score = 76.3 bits (186), Expect = 2e-11
 Identities = 38/89 (42%), Positives = 53/89 (59%), Gaps = 3/89 (3%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTS-SGGKCPQCNTSCAYKDVILLYASRASAHQKTSSTRHF 728
           + CLPCGH+YGFSCIK W+Q S S GKCPQC   C  KDV +LYASR     +    R +
Sbjct: 155 ISCLPCGHIYGFSCIKTWIQQSPSSGKCPQCKKLCTLKDVRVLYASRLCVVDEKLQKRVY 214

Query: 727 SFTKQ--GIREFREHERLRRVDTEEMHAE 647
           S   +   +++   +E +R    + M+A+
Sbjct: 215 SLEAECAYLKQKTVYEDMREEMRKHMYAK 243


>ref|XP_023746023.1| uncharacterized protein LOC111894169 [Lactuca sativa]
          Length = 181

 Score = 92.8 bits (229), Expect = 4e-18
 Identities = 36/65 (55%), Positives = 48/65 (73%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC++     G+H++CCLPCGH+YGFSCI KWL +S  S KCPQC  +C  KDV +L+A
Sbjct: 34   CPICFEAWTSGGEHQICCLPCGHIYGFSCINKWLKRSPNSRKCPQCKKLCKLKDVRVLYA 93

Query: 1444 STLCI 1430
            + LC+
Sbjct: 94   ARLCV 98



 Score = 74.7 bits (182), Expect = 8e-12
 Identities = 37/91 (40%), Positives = 50/91 (54%), Gaps = 1/91 (1%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTS-SGGKCPQCNTSCAYKDVILLYASRASAHQKTSSTRHF 728
           +CCLPCGH+YGFSCI KWL+ S +  KCPQC   C  KDV +LYA+R     +    R  
Sbjct: 49  ICCLPCGHIYGFSCINKWLKRSPNSRKCPQCKKLCKLKDVRVLYAARLCVADEELQKRVI 108

Query: 727 SFTKQGIREFREHERLRRVDTEEMHAEDSKK 635
           +   +     + H+ +     EEM  E  +K
Sbjct: 109 TLEAECAYLKQSHKSVYERVLEEMRNEMDEK 139


>ref|XP_023745920.1| E3 ubiquitin-protein ligase RFWD3-like [Lactuca sativa]
          Length = 325

 Score = 94.7 bits (234), Expect = 2e-17
 Identities = 52/170 (30%), Positives = 86/170 (50%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC++     G H++CCLPCGH+YG SCI KWL     SGKCPQC   C  KDV +L+A
Sbjct: 164  CPICFEAWTSGGSHQICCLPCGHIYGLSCINKWLQLRRSSGKCPQCKKSCTLKDVRVLYA 223

Query: 1444 STLCIHAHQKATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYTDDYMRMVDVVEQQRDLA 1265
            + LC+                    + + H+ +R L+A+  Y +    +  + E+ R+  
Sbjct: 224  ARLCV-------------------ADEELHKKVRCLEAKCAYLEQKDYVERIKEELRE-- 262

Query: 1264 KQQAAMKPQWDDLVARSVEMNQRAEKLGQRFDELEGADAFKQQIRALTRL 1115
            + +  M  +W+ L     EM ++ +KL +  +E+   +   + +R   +L
Sbjct: 263  ELERDMDEKWEKL---KREMVEKMDKLERDMEEIRMVNEIDEYVRNQNQL 309



 Score = 76.6 bits (187), Expect = 2e-11
 Identities = 32/47 (68%), Positives = 36/47 (76%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQ-TSSGGKCPQCNTSCAYKDVILLYASR 767
           +CCLPCGH+YG SCI KWLQ   S GKCPQC  SC  KDV +LYA+R
Sbjct: 179 ICCLPCGHIYGLSCINKWLQLRRSSGKCPQCKKSCTLKDVRVLYAAR 225


>gb|PLY75050.1| hypothetical protein LSAT_2X30721 [Lactuca sativa]
          Length = 608

 Score = 95.9 bits (237), Expect = 5e-17
 Identities = 48/129 (37%), Positives = 70/129 (54%), Gaps = 29/129 (22%)
 Frame = -1

Query: 1729 MTSPRISCWLGADGKTYQGVMVVDD----------------------DIGNKCQYDD--- 1625
            +TSPR+S  +   G + + V++ DD                      D G++ +++    
Sbjct: 43   VTSPRVSNVVSRAGNSQEAVVLHDDLVEESNCKRSCKGNLGSQESGRDDGDEDKFNGGEI 102

Query: 1624 ----CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVV 1457
                C IC +     G+H++CCLPCGH+YG SCIKKWL Q   SGKCPQCN  C  KD+ 
Sbjct: 103  DGLFCPICIEAWTSGGEHQICCLPCGHIYGLSCIKKWLRQRRGSGKCPQCNKKCTLKDIR 162

Query: 1456 LLHASTLCI 1430
            +L+A+ LC+
Sbjct: 163  VLYAARLCV 171



 Score = 77.8 bits (190), Expect = 3e-11
 Identities = 31/47 (65%), Positives = 37/47 (78%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTSSG-GKCPQCNTSCAYKDVILLYASR 767
           +CCLPCGH+YG SCIKKWL+   G GKCPQCN  C  KD+ +LYA+R
Sbjct: 122 ICCLPCGHIYGLSCIKKWLRQRRGSGKCPQCNKKCTLKDIRVLYAAR 168


>ref|XP_023732273.1| E3 ubiquitin-protein ligase RFWD3-like [Lactuca sativa]
          Length = 614

 Score = 95.9 bits (237), Expect = 5e-17
 Identities = 48/129 (37%), Positives = 70/129 (54%), Gaps = 29/129 (22%)
 Frame = -1

Query: 1729 MTSPRISCWLGADGKTYQGVMVVDD----------------------DIGNKCQYDD--- 1625
            +TSPR+S  +   G + + V++ DD                      D G++ +++    
Sbjct: 43   VTSPRVSNVVSRAGNSQEAVVLHDDLVEESNCKRSCKGNLGSQESGRDDGDEDKFNGGEI 102

Query: 1624 ----CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVV 1457
                C IC +     G+H++CCLPCGH+YG SCIKKWL Q   SGKCPQCN  C  KD+ 
Sbjct: 103  DGLFCPICIEAWTSGGEHQICCLPCGHIYGLSCIKKWLRQRRGSGKCPQCNKKCTLKDIR 162

Query: 1456 LLHASTLCI 1430
            +L+A+ LC+
Sbjct: 163  VLYAARLCV 171



 Score = 77.8 bits (190), Expect = 3e-11
 Identities = 31/47 (65%), Positives = 37/47 (78%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTSSG-GKCPQCNTSCAYKDVILLYASR 767
           +CCLPCGH+YG SCIKKWL+   G GKCPQCN  C  KD+ +LYA+R
Sbjct: 122 ICCLPCGHIYGLSCIKKWLRQRRGSGKCPQCNKKCTLKDIRVLYAAR 168


>gb|PLY64540.1| hypothetical protein LSAT_6X24480 [Lactuca sativa]
          Length = 311

 Score = 92.4 bits (228), Expect = 8e-17
 Identities = 36/65 (55%), Positives = 47/65 (72%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC++     G H++CCLPCGH+YGFSCI KWL +S  S KCPQC  +C  KDV +L+A
Sbjct: 164  CPICFEAWTSGGSHQICCLPCGHIYGFSCINKWLKRSPNSRKCPQCKKLCKLKDVRVLYA 223

Query: 1444 STLCI 1430
            + LC+
Sbjct: 224  ARLCV 228



 Score = 74.7 bits (182), Expect = 7e-11
 Identities = 37/91 (40%), Positives = 50/91 (54%), Gaps = 1/91 (1%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWLQTS-SGGKCPQCNTSCAYKDVILLYASRASAHQKTSSTRHF 728
           +CCLPCGH+YGFSCI KWL+ S +  KCPQC   C  KDV +LYA+R     +    R  
Sbjct: 179 ICCLPCGHIYGFSCINKWLKRSPNSRKCPQCKKLCKLKDVRVLYAARLCVADEELQKRVI 238

Query: 727 SFTKQGIREFREHERLRRVDTEEMHAEDSKK 635
           +   +     + H+ +     EEM  E  +K
Sbjct: 239 TLEAECAYLKQSHKSVYERVLEEMRNEMDEK 269


>ref|XP_022009012.1| E3 ubiquitin-protein ligase RFWD3 isoform X2 [Helianthus annuus]
 gb|OTF97287.1| putative transducin/WD40 repeat-like superfamily protein [Helianthus
            annuus]
          Length = 565

 Score = 94.7 bits (234), Expect = 1e-16
 Identities = 56/161 (34%), Positives = 87/161 (54%), Gaps = 6/161 (3%)
 Frame = -1

Query: 1726 TSPRISCWLGADGKTYQGVMVV-DDDIGNKCQYDDCSICYQPMNIRGKHRLCCLPCGHLY 1550
            +S R+S   G D K+ + V+V  DDD     +   C IC +P    G H++ CLPCGH+Y
Sbjct: 39   SSSRVS---GNDKKSSKEVVVCGDDDFNLDIEGLCCPICTEPWTTGGLHQISCLPCGHIY 95

Query: 1549 GFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHASTLC-----IHAHQKATSARYFPFT 1385
            G SCIKKW+ Q S S KCPQCN  C  KDV +L+A  L      +    ++  A+     
Sbjct: 96   GLSCIKKWIQQRSRSAKCPQCNKKCTRKDVRVLYAEQLHVVDAEVQKQVQSLEAKCASLE 155

Query: 1384 EQGFIEFKEHEWLRHLDAQKRYTDDYMRMVDVVEQQRDLAK 1262
            ++  +  K     + ++AQKR  D ++++  + E+ + L +
Sbjct: 156  QKNAVCCK-----KEVEAQKREADLHLKVAKLTEKTKYLER 191



 Score = 70.1 bits (170), Expect = 7e-09
 Identities = 29/47 (61%), Positives = 34/47 (72%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASR 767
           + CLPCGH+YG SCIKKW+ Q S   KCPQCN  C  KDV +LYA +
Sbjct: 86  ISCLPCGHIYGLSCIKKWIQQRSRSAKCPQCNKKCTRKDVRVLYAEQ 132


>ref|XP_020212865.1| E3 ubiquitin-protein ligase RFWD3-like isoform X2 [Cajanus cajan]
          Length = 304

 Score = 91.3 bits (225), Expect = 2e-16
 Identities = 64/219 (29%), Positives = 105/219 (47%), Gaps = 6/219 (2%)
 Frame = -1

Query: 1660 DDDIGNKCQYDD--CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQC 1487
            DD+  N+ + D   C IC      +G+H +CCLPCGH+YG SCIK+WL + + S  CPQC
Sbjct: 94   DDNAWNRTEIDGLVCPICMDVWTNKGEHHICCLPCGHIYGMSCIKRWLQRRAGSANCPQC 153

Query: 1486 NFICAYKDVVLLHASTLCIHAHQKATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYTDDY 1307
            N  C+ KDV  L+AS +                     ++ + H+ +R L+A+    +  
Sbjct: 154  NRKCSMKDVRKLYASRVVA-------------------VDEESHKRIRLLEAKCIALES- 193

Query: 1306 MRMVDVVEQQRDLAKQQAAMKPQWDDLVARSVEMNQRAEKLGQRFDELEGADAFKQQIRA 1127
             +  D  +++    K +AA+  Q   L   S++    ++K GQ ++    AD F    R 
Sbjct: 194  -KDTDWQKKEAGWQKSEAALHLQVQKLTEASLKDIADSKKQGQVYNT---ADLFANVWRG 249

Query: 1126 LTRLNGSLEKRYNA----LGRHTDALKQRTNVLAQRYNA 1022
            ++ L+ S  +  +A    L       +QR  +L Q+  A
Sbjct: 250  MSSLSESQNQIPSAGSVTLEERITRTEQRIAILEQQIQA 288



 Score = 78.6 bits (192), Expect = 3e-12
 Identities = 36/63 (57%), Positives = 42/63 (66%), Gaps = 1/63 (1%)
 Frame = -1

Query: 919 HHASALCCLPCGHLYGFSCIKKWLQTSSG-GKCPQCNTSCAYKDVILLYASRASAHQKTS 743
           HH   +CCLPCGH+YG SCIK+WLQ  +G   CPQCN  C+ KDV  LYASR  A  + S
Sbjct: 121 HH---ICCLPCGHIYGMSCIKRWLQRRAGSANCPQCNRKCSMKDVRKLYASRVVAVDEES 177

Query: 742 STR 734
             R
Sbjct: 178 HKR 180


>ref|XP_004494071.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3 [Cicer arietinum]
          Length = 660

 Score = 94.4 bits (233), Expect = 2e-16
 Identities = 65/191 (34%), Positives = 83/191 (43%), Gaps = 24/191 (12%)
 Frame = -1

Query: 1645 NKCQYDD-----CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNF 1481
            N+C   D     C IC      +G H +CCLPCGH+YG SCIKKWL Q   SGKCPQCN 
Sbjct: 142  NECNLADIDGLICPICMDAWTDKGDHHVCCLPCGHIYGMSCIKKWLQQRKNSGKCPQCNR 201

Query: 1480 ICAYKDVVLLHASTLCIHAHQKATSARYFPF------TEQGFIEFKEHEWLR-----HLD 1334
             C+ KDV  L+AS +     +     R          ++ G    KE  W +     HLD
Sbjct: 202  KCSMKDVRKLYASRIVAVDEESQKRIRSLEIKCATLESKDGDWRMKEAGWKKRESALHLD 261

Query: 1333 AQK-RYTDDYMRMVDVVEQQRDLAKQQAAMKPQWDDLVARSVEMNQRAEKLG-------Q 1178
             +  +    Y+  + +  Q R      A    QW     RS   N R+   G       Q
Sbjct: 262  VKNLKQRQTYLEQLVLDMQSRQSGLMDANGNSQWRYEPERS--CNPRSHGKGSFCNFEFQ 319

Query: 1177 RFDELEGADAF 1145
            +   LEGA  F
Sbjct: 320  KAFHLEGARVF 330



 Score = 81.6 bits (200), Expect = 2e-12
 Identities = 38/63 (60%), Positives = 43/63 (68%), Gaps = 1/63 (1%)
 Frame = -1

Query: 919 HHASALCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASRASAHQKTS 743
           HH   +CCLPCGH+YG SCIKKWL Q  + GKCPQCN  C+ KDV  LYASR  A  + S
Sbjct: 167 HH---VCCLPCGHIYGMSCIKKWLQQRKNSGKCPQCNRKCSMKDVRKLYASRIVAVDEES 223

Query: 742 STR 734
             R
Sbjct: 224 QKR 226


>ref|XP_022009011.1| E3 ubiquitin-protein ligase RFWD3 isoform X1 [Helianthus annuus]
          Length = 566

 Score = 94.0 bits (232), Expect = 2e-16
 Identities = 56/158 (35%), Positives = 87/158 (55%), Gaps = 3/158 (1%)
 Frame = -1

Query: 1726 TSPRISCWLGADGKTYQGVMVV-DDDIGNKCQYDDCSICYQPMNIRGKHRLCCLPCGHLY 1550
            +S R+S   G D K+ + V+V  DDD     +   C IC +P    G H++ CLPCGH+Y
Sbjct: 39   SSSRVS---GNDKKSSKEVVVCGDDDFNLDIEGLCCPICTEPWTTGGLHQISCLPCGHIY 95

Query: 1549 GFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHASTLCIHAHQKATSARYFPFTEQGFI 1370
            G SCIKKW+ Q S S KCPQCN  C  KDV +L+A  L +   +     +     +   +
Sbjct: 96   GLSCIKKWIQQRSRSAKCPQCNKKCTRKDVRVLYAEQLHVVDAEVQKQVQSLE-AKCASL 154

Query: 1369 EFKEHE--WLRHLDAQKRYTDDYMRMVDVVEQQRDLAK 1262
            E K++     + ++AQKR  D ++++  + E+ + L +
Sbjct: 155  EQKQNAVCCKKEVEAQKREADLHLKVAKLTEKTKYLER 192



 Score = 70.1 bits (170), Expect = 7e-09
 Identities = 29/47 (61%), Positives = 34/47 (72%), Gaps = 1/47 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASR 767
           + CLPCGH+YG SCIKKW+ Q S   KCPQCN  C  KDV +LYA +
Sbjct: 86  ISCLPCGHIYGLSCIKKWIQQRSRSAKCPQCNKKCTRKDVRVLYAEQ 132


>gb|PNX94327.1| E3 ubiquitin-protein ligase rfwd3-like protein [Trifolium pratense]
          Length = 425

 Score = 92.8 bits (229), Expect = 2e-16
 Identities = 53/142 (37%), Positives = 67/142 (47%), Gaps = 12/142 (8%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC       G H +CCLPCGH+YG SCIKKWL Q   SGKCPQCN  C+ KDV  L+A
Sbjct: 161  CPICMDAWTENGDHHVCCLPCGHIYGMSCIKKWLQQRKNSGKCPQCNMKCSMKDVRKLYA 220

Query: 1444 STLCIHAHQKATSARYFPF------TEQGFIEFKEHEWLR-----HLDAQK-RYTDDYMR 1301
            S +     +     R          ++ G    KE  W +     HLD QK    + Y+ 
Sbjct: 221  SRIVAVDEESQKRIRSLESKCVSLESKDGSWRKKEMGWKKREAELHLDVQKLTKRNTYLE 280

Query: 1300 MVDVVEQQRDLAKQQAAMKPQW 1235
             + +  Q+R      A    QW
Sbjct: 281  QLVLDMQRRQSELMVAGGNSQW 302



 Score = 81.6 bits (200), Expect = 9e-13
 Identities = 38/63 (60%), Positives = 43/63 (68%), Gaps = 1/63 (1%)
 Frame = -1

Query: 919 HHASALCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASRASAHQKTS 743
           HH   +CCLPCGH+YG SCIKKWL Q  + GKCPQCN  C+ KDV  LYASR  A  + S
Sbjct: 174 HH---VCCLPCGHIYGMSCIKKWLQQRKNSGKCPQCNMKCSMKDVRKLYASRIVAVDEES 230

Query: 742 STR 734
             R
Sbjct: 231 QKR 233


>gb|OVA06944.1| zinc finger protein [Macleaya cordata]
          Length = 229

 Score = 89.0 bits (219), Expect = 3e-16
 Identities = 56/182 (30%), Positives = 97/182 (53%), Gaps = 14/182 (7%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQS-SFSGKCPQCNFICAYKDVVLLH 1448
            CSIC +P +  G H++ CLPCGHL+G SCIK+W+ QS   + KCP+CN  CA KD++ L+
Sbjct: 33   CSICMEPWSSVGNHQVSCLPCGHLFGLSCIKRWIQQSRRNNSKCPKCNRKCALKDIIRLY 92

Query: 1447 ASTLCIHAHQKATSARYFPFTEQGFIEFKEHEWLR-----------HLDAQKRYTDDYMR 1301
             S L +  + +    R    T +  +E    +  R           +++A  R+ +   R
Sbjct: 93   VSGLAVANYDEELEKRL--LTLEAAMEKTNRDLQRKAEEQNSIIEANIEANGRWMEAQNR 150

Query: 1300 MVDVVEQ--QRDLAKQQAAMKPQWDDLVARSVEMNQRAEKLGQRFDELEGADAFKQQIRA 1127
             +  ++   QR + +Q + +     + ++R +E+N R  ++GQ  + L+  D+   +IR 
Sbjct: 151  TLKQMKHDLQRTIEEQNSKI-----EAISRKLEVNDR--RIGQLMEALKANDS---RIRQ 200

Query: 1126 LT 1121
            LT
Sbjct: 201  LT 202



 Score = 69.3 bits (168), Expect = 1e-09
 Identities = 28/45 (62%), Positives = 34/45 (75%), Gaps = 2/45 (4%)
 Frame = -1

Query: 898 CLPCGHLYGFSCIKKWLQTS--SGGKCPQCNTSCAYKDVILLYAS 770
           CLPCGHL+G SCIK+W+Q S  +  KCP+CN  CA KD+I LY S
Sbjct: 50  CLPCGHLFGLSCIKRWIQQSRRNNSKCPKCNRKCALKDIIRLYVS 94


>ref|XP_010543439.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3 [Tarenaya hassleriana]
          Length = 623

 Score = 93.2 bits (230), Expect = 4e-16
 Identities = 50/127 (39%), Positives = 70/127 (55%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC +P    G+H +CCLPCGHLYGFSCIKKWL Q   SGKCPQCN  C+ KDV  + A
Sbjct: 118  CLICMEPWTNGGEHHICCLPCGHLYGFSCIKKWLQQRRSSGKCPQCNRKCSVKDVRKIFA 177

Query: 1444 STLCIHAHQKATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYTDDYMRMVDVVEQQRDLA 1265
            S +     +      Y    +   +E KE  W +  +A+ R  +  +R  +V + ++ + 
Sbjct: 178  SRIAAVDGESHKRILYLE-AKLSSLEKKEAGWGKK-EAEWRKVEAELRS-EVNQLRKSIV 234

Query: 1264 KQQAAMK 1244
              +  MK
Sbjct: 235  SMENMMK 241



 Score = 83.2 bits (204), Expect = 6e-13
 Identities = 39/63 (61%), Positives = 44/63 (69%), Gaps = 1/63 (1%)
 Frame = -1

Query: 919 HHASALCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASRASAHQKTS 743
           HH   +CCLPCGHLYGFSCIKKWL Q  S GKCPQCN  C+ KDV  ++ASR +A    S
Sbjct: 131 HH---ICCLPCGHLYGFSCIKKWLQQRRSSGKCPQCNRKCSVKDVRKIFASRIAAVDGES 187

Query: 742 STR 734
             R
Sbjct: 188 HKR 190


>ref|XP_023895811.1| E3 ubiquitin-protein ligase RFWD3 isoform X2 [Quercus suber]
          Length = 604

 Score = 92.4 bits (228), Expect = 6e-16
 Identities = 56/173 (32%), Positives = 78/173 (45%), Gaps = 2/173 (1%)
 Frame = -1

Query: 1657 DDIGNKCQYDD--CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCN 1484
            D+  N+ + D   C IC +     G H +CCLPCGH+YG SCIKKWL Q   +GKCPQCN
Sbjct: 82   DNEWNRSEIDGLFCPICMEAWTTDGDHYICCLPCGHIYGMSCIKKWLQQRRNAGKCPQCN 141

Query: 1483 FICAYKDVVLLHASTLCIHAHQKATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYTDDYM 1304
              C  KDV  L AS +     +     R     +   +E K+  W +     K+  DD  
Sbjct: 142  RACTLKDVRKLFASRVVAVDEESQKRIRSLE-AKCASLEKKDANWCKKESEWKKREDDLH 200

Query: 1303 RMVDVVEQQRDLAKQQAAMKPQWDDLVARSVEMNQRAEKLGQRFDELEGADAF 1145
            R V             + +K +  DL     ++  RA +L       +G+  F
Sbjct: 201  RKV-------------SQLKKRTTDLEHLLGDVQSRASRLANAGGHYKGSPVF 240



 Score = 79.7 bits (195), Expect = 7e-12
 Identities = 35/58 (60%), Positives = 41/58 (70%), Gaps = 1/58 (1%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASRASAHQKTSSTR 734
           +CCLPCGH+YG SCIKKWL Q  + GKCPQCN +C  KDV  L+ASR  A  + S  R
Sbjct: 110 ICCLPCGHIYGMSCIKKWLQQRRNAGKCPQCNRACTLKDVRKLFASRVVAVDEESQKR 167


>ref|XP_023895810.1| E3 ubiquitin-protein ligase RFWD3 isoform X1 [Quercus suber]
          Length = 605

 Score = 92.4 bits (228), Expect = 6e-16
 Identities = 56/173 (32%), Positives = 78/173 (45%), Gaps = 2/173 (1%)
 Frame = -1

Query: 1657 DDIGNKCQYDD--CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCN 1484
            D+  N+ + D   C IC +     G H +CCLPCGH+YG SCIKKWL Q   +GKCPQCN
Sbjct: 82   DNEWNRSEIDGLFCPICMEAWTTDGDHYICCLPCGHIYGMSCIKKWLQQRRNAGKCPQCN 141

Query: 1483 FICAYKDVVLLHASTLCIHAHQKATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYTDDYM 1304
              C  KDV  L AS +     +     R     +   +E K+  W +     K+  DD  
Sbjct: 142  RACTLKDVRKLFASRVVAVDEESQKRIRSLE-AKCASLEKKDANWCKKESEWKKREDDLH 200

Query: 1303 RMVDVVEQQRDLAKQQAAMKPQWDDLVARSVEMNQRAEKLGQRFDELEGADAF 1145
            R V             + +K +  DL     ++  RA +L       +G+  F
Sbjct: 201  RKV-------------SQLKKRTTDLEHLLGDVQSRASRLANAGGHYKGSPVF 240



 Score = 79.7 bits (195), Expect = 7e-12
 Identities = 35/58 (60%), Positives = 41/58 (70%), Gaps = 1/58 (1%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASRASAHQKTSSTR 734
           +CCLPCGH+YG SCIKKWL Q  + GKCPQCN +C  KDV  L+ASR  A  + S  R
Sbjct: 110 ICCLPCGHIYGMSCIKKWLQQRRNAGKCPQCNRACTLKDVRKLFASRVVAVDEESQKR 167


>ref|XP_018470955.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3-like [Raphanus sativus]
          Length = 628

 Score = 92.4 bits (228), Expect = 7e-16
 Identities = 58/163 (35%), Positives = 81/163 (49%)
 Frame = -1

Query: 1624 CSICYQPMNIRGKHRLCCLPCGHLYGFSCIKKWLLQSSFSGKCPQCNFICAYKDVVLLHA 1445
            C IC +     G+H++CCLPCGHLYGFSCIKKWL Q   +GKCPQCN IC  +DV  + A
Sbjct: 125  CLICMEVWTDGGEHQVCCLPCGHLYGFSCIKKWLKQPRSAGKCPQCNRICGLRDVRKIFA 184

Query: 1444 STLCIHAHQKATSARYFPFTEQGFIEFKEHEWLRHLDAQKRYTDDYMRMVDVVEQQRDLA 1265
            S +       A     F   +    E K   W    +AQ R      R  ++  +  +L 
Sbjct: 185  SRIAA-VDDDAHKRILFLEGKLNLFEQKTASW-SSKEAQWR-----KREAELRSEVNNLK 237

Query: 1264 KQQAAMKPQWDDLVARSVEMNQRAEKLGQRFDELEGADAFKQQ 1136
            K+ A M+   +  V  S E     +K+ Q  + L  + +F+ Q
Sbjct: 238  KKIAYMESTTNGAVRASQEKYIPGQKIYQERNGLAPSCSFRHQ 280



 Score = 79.3 bits (194), Expect = 1e-11
 Identities = 34/50 (68%), Positives = 39/50 (78%), Gaps = 1/50 (2%)
 Frame = -1

Query: 904 LCCLPCGHLYGFSCIKKWL-QTSSGGKCPQCNTSCAYKDVILLYASRASA 758
           +CCLPCGHLYGFSCIKKWL Q  S GKCPQCN  C  +DV  ++ASR +A
Sbjct: 140 VCCLPCGHLYGFSCIKKWLKQPRSAGKCPQCNRICGLRDVRKIFASRIAA 189