BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cheilocostus21_contig00034941
         (2136 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_009417506.1| PREDICTED: isoamylase 1, chloroplastic isofo...  1295   0.0  
ref|XP_008798152.1| PREDICTED: isoamylase 1, chloroplastic isofo...  1264   0.0  
ref|XP_010927188.1| PREDICTED: isoamylase 1, chloroplastic isofo...  1249   0.0  
ref|XP_010263294.1| PREDICTED: isoamylase 1, chloroplastic [Nelu...  1240   0.0  
ref|XP_020110388.1| isoamylase 1, chloroplastic isoform X1 [Anan...  1239   0.0  
ref|XP_012075534.1| isoamylase 1, chloroplastic [Jatropha curcas...  1223   0.0  
gb|AUZ20772.1| isoamylase 1 (plastid) [Manihot esculenta]            1213   0.0  
ref|XP_021614773.1| isoamylase 1, chloroplastic [Manihot esculen...  1212   0.0  
ref|XP_010048547.1| PREDICTED: isoamylase 1, chloroplastic isofo...  1211   0.0  
ref|XP_020265323.1| isoamylase 1, chloroplastic isoform X3 [Aspa...  1211   0.0  
ref|XP_021277024.1| isoamylase 1, chloroplastic isoform X1 [Herr...  1207   0.0  
ref|XP_007014466.2| PREDICTED: isoamylase 1, chloroplastic isofo...  1207   0.0  
gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]              1207   0.0  
ref|XP_022134724.1| isoamylase 1, chloroplastic [Momordica chara...  1206   0.0  
ref|XP_014510281.1| isoamylase 1, chloroplastic [Vigna radiata v...  1206   0.0  
ref|XP_020265321.1| isoamylase 1, chloroplastic isoform X1 [Aspa...  1205   0.0  
ref|XP_020215619.1| isoamylase 1, chloroplastic [Cajanus cajan]      1204   0.0  
gb|PIA43915.1| hypothetical protein AQUCO_01800158v1 [Aquilegia ...  1202   0.0  
ref|XP_017615133.1| PREDICTED: isoamylase 1, chloroplastic [Goss...  1202   0.0  
ref|XP_016719009.1| PREDICTED: isoamylase 1, chloroplastic-like ...  1202   0.0  

>ref|XP_009417506.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Musa acuminata
            subsp. malaccensis]
          Length = 795

 Score = 1295 bits (3350), Expect = 0.0
 Identities = 593/709 (83%), Positives = 649/709 (91%)
 Frame = +1

Query: 1    SPVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHI 180
            SP PLGATAC+GGVNFAIYSS ATAAALCLFT+ DL ANRVT+EVVLDPL+N+TGN+WH+
Sbjct: 86   SPAPLGATACDGGVNFAIYSSGATAAALCLFTLSDLTANRVTEEVVLDPLINRTGNVWHV 145

Query: 181  FLQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQM 360
            FLQG  +DMVYGY+FDGK SP EG YFDYSR+LLDPYAKAV+SRG+YGV G   +CWPQM
Sbjct: 146  FLQGELDDMVYGYKFDGKLSPREGHYFDYSRILLDPYAKAVISRGEYGVLGPGGECWPQM 205

Query: 361  AGMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHL 540
            AGMIPVS+++FDW+GDLPL+YPQKDL+IYEMHVRGFT  +SS    PGTY+  I+KLDHL
Sbjct: 206  AGMIPVSNEEFDWEGDLPLQYPQKDLIIYEMHVRGFTKHDSSEIDSPGTYISAIKKLDHL 265

Query: 541  KDLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGR 720
            K+LG+NCIELMPCHEFNELEYFSYN V   NK+NFWGYST++YFSPMIRYSS G+ANCGR
Sbjct: 266  KELGINCIELMPCHEFNELEYFSYNSVLGENKLNFWGYSTVSYFSPMIRYSSAGLANCGR 325

Query: 721  DAINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYN 900
            DAINEFKTL+REAHKRGIEVLLDVVFNHTAEGNENGP+ SFRGIDNSTYYM+APKGEFYN
Sbjct: 326  DAINEFKTLIREAHKRGIEVLLDVVFNHTAEGNENGPMISFRGIDNSTYYMLAPKGEFYN 385

Query: 901  YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPI 1080
            YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRS SLWDPVN YG PI
Sbjct: 386  YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSCSLWDPVNVYGNPI 445

Query: 1081 EGDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKY 1260
            EGDM+TTG  +  PP+I MISNDPILCKVKLIAEAWDAGGLYQVGSFPH  IWSEWNG+Y
Sbjct: 446  EGDMVTTGTPVGGPPVIEMISNDPILCKVKLIAEAWDAGGLYQVGSFPHSGIWSEWNGQY 505

Query: 1261 RDIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNK 1440
            RDIVRQFIKGTDGF+GA AQCLCGSP+LYQDGGRKPWHSINFICAHDGFTLADLVTYNNK
Sbjct: 506  RDIVRQFIKGTDGFAGAFAQCLCGSPNLYQDGGRKPWHSINFICAHDGFTLADLVTYNNK 565

Query: 1441 HNLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 1620
             NL NGEDNRDGENHNLSWNCGEEG   SI VRRLRKRQMRNFFLCLMVSQGVPMIYMGD
Sbjct: 566  SNLSNGEDNRDGENHNLSWNCGEEGEFASITVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 625

Query: 1621 EYGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAE 1800
            EYGH+KGGNNN+YCHDN  NYF WD+KE++S DFYRFCRL+IKFR ECESLGL EFPTAE
Sbjct: 626  EYGHTKGGNNNTYCHDNYFNYFRWDRKEESSSDFYRFCRLMIKFRYECESLGLDEFPTAE 685

Query: 1801 RLKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLV 1980
            RL+WHGHQP  PDWS+TSRFVAF++SD++KGEIYVAFNTSHLPVTV LPER GYRWEPLV
Sbjct: 686  RLQWHGHQPKTPDWSETSRFVAFTMSDSLKGEIYVAFNTSHLPVTVTLPERSGYRWEPLV 745

Query: 1981 DTSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            DTSKP PYDFLS D+PDRA A+K YS+FLD + YPMLSYSSV+L+L+PD
Sbjct: 746  DTSKPVPYDFLSDDVPDRATAVKLYSHFLDANLYPMLSYSSVILILQPD 794


>ref|XP_008798152.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Phoenix
            dactylifera]
          Length = 803

 Score = 1264 bits (3271), Expect = 0.0
 Identities = 579/708 (81%), Positives = 633/708 (89%)
 Frame = +1

Query: 1    SPVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHI 180
            +P PLGATACNGGVNFAI SS+A+A  LCLFT+ DL+ANRVT+++ LDPL NKTG++WH+
Sbjct: 93   TPAPLGATACNGGVNFAICSSSASAVTLCLFTLSDLKANRVTEQISLDPLFNKTGDMWHV 152

Query: 181  FLQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQM 360
            F+QG F DM+YGY+FDGKFSPEEG  FDYSR+LLDPYAKAV+SRG YGV G   DCWPQM
Sbjct: 153  FVQGEFRDMLYGYKFDGKFSPEEGHSFDYSRILLDPYAKAVISRGDYGVLGPGGDCWPQM 212

Query: 361  AGMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHL 540
            AGMIP+S D+FDW+GDLPL Y QKDL+IYEMHVRGFT  +SS T  PGTYLG   KLDHL
Sbjct: 213  AGMIPISDDEFDWEGDLPLNYAQKDLIIYEMHVRGFTRHDSSRTDSPGTYLGAANKLDHL 272

Query: 541  KDLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGR 720
            K+LGVNCIELMPC EFNELEYFSYN +   NK+NFWGYST+NYFSPMIRYSS G+ NCG 
Sbjct: 273  KELGVNCIELMPCQEFNELEYFSYNSILGENKLNFWGYSTVNYFSPMIRYSSAGMNNCGL 332

Query: 721  DAINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYN 900
            DAINEFKTLVREAHK+GIEVLLDVVFNHTAEGNENGPI SFRGIDNSTYYM+APKGE YN
Sbjct: 333  DAINEFKTLVREAHKKGIEVLLDVVFNHTAEGNENGPILSFRGIDNSTYYMLAPKGESYN 392

Query: 901  YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPI 1080
            YSGCGNTFNCNHP VRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVN YG PI
Sbjct: 393  YSGCGNTFNCNHPTVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNVYGNPI 452

Query: 1081 EGDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKY 1260
            EGD  TTG  L +PPLI M+SNDPIL KVKLIAEAWDAGGLYQVG FPHW IWSEWNG+Y
Sbjct: 453  EGDPFTTGTPLGSPPLIDMMSNDPILSKVKLIAEAWDAGGLYQVGDFPHWSIWSEWNGQY 512

Query: 1261 RDIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNK 1440
            RD VRQFIKGTDGFSGA AQCLCGSP+LYQ GGRKPWHSINF+CAHDGFTLADLVTYNNK
Sbjct: 513  RDTVRQFIKGTDGFSGAFAQCLCGSPNLYQGGGRKPWHSINFVCAHDGFTLADLVTYNNK 572

Query: 1441 HNLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 1620
            +NL NGEDNRDGENHNLSWNCGEEG   SI VRRLRKRQMRNFFLCLMVSQGVPMIYMGD
Sbjct: 573  YNLSNGEDNRDGENHNLSWNCGEEGEFASITVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 632

Query: 1621 EYGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAE 1800
            EYGH+KGGNNN+YCHDN +NYF WD+KE++S DFYRFCRL+ KFR ECESLGL +FPTAE
Sbjct: 633  EYGHTKGGNNNTYCHDNYLNYFRWDRKEESSSDFYRFCRLMTKFRQECESLGLDDFPTAE 692

Query: 1801 RLKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLV 1980
            RL+WHGHQP  PDWS TSRFVAFS+ DAVKGEIYVAFNTSHLPVT+ LP+R GYRWEPLV
Sbjct: 693  RLQWHGHQPGMPDWSKTSRFVAFSMMDAVKGEIYVAFNTSHLPVTIMLPDRPGYRWEPLV 752

Query: 1981 DTSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRP 2124
            DT KP PYDFLS DLPDRAIA+KQY++FL+ + YPMLSYSS++L+L P
Sbjct: 753  DTGKPTPYDFLSDDLPDRAIAVKQYAHFLNSNLYPMLSYSSIILMLHP 800


>ref|XP_010927188.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Elaeis guineensis]
          Length = 803

 Score = 1249 bits (3233), Expect = 0.0
 Identities = 571/708 (80%), Positives = 630/708 (88%)
 Frame = +1

Query: 1    SPVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHI 180
            +P PLGATACNGGVNFAI SS+A+AA LCLFT+ DL+ANRVT+++ LDPL NKTG+IWH+
Sbjct: 93   TPAPLGATACNGGVNFAICSSSASAATLCLFTLSDLKANRVTEQISLDPLFNKTGDIWHV 152

Query: 181  FLQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQM 360
            F+QG F+DM+YGY+FDG+FSPEEG YFDYSR+LLDPYAKAV+SRG YGV     DCW QM
Sbjct: 153  FVQGEFKDMLYGYKFDGRFSPEEGHYFDYSRILLDPYAKAVISRGDYGVLEPGGDCWSQM 212

Query: 361  AGMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHL 540
            AGMIP+S D+FDW+GDLPL YPQKDL+IYEMHVRGFT  +SS T  PGTY G   KLDHL
Sbjct: 213  AGMIPISDDEFDWEGDLPLNYPQKDLIIYEMHVRGFTRHDSSQTDSPGTYRGAANKLDHL 272

Query: 541  KDLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGR 720
            ++LGVNCIELMPC EFNELEYFS+N +   NK NFWGYST+NYFSPMIRYSS G+ NCG 
Sbjct: 273  EELGVNCIELMPCQEFNELEYFSFNSILGENKSNFWGYSTVNYFSPMIRYSSAGMNNCGL 332

Query: 721  DAINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYN 900
            DAINEFKTLV+EAHKRGIEVLLDVVFNHTAEGNENGPI SFRGIDNSTYYM+APKG FYN
Sbjct: 333  DAINEFKTLVKEAHKRGIEVLLDVVFNHTAEGNENGPILSFRGIDNSTYYMLAPKGGFYN 392

Query: 901  YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPI 1080
            YSGCGNTFNCNHP VRQFI+DCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVN YG PI
Sbjct: 393  YSGCGNTFNCNHPAVRQFIIDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNVYGHPI 452

Query: 1081 EGDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKY 1260
            E D  TTG  L +PPLI M+SNDPIL KVKLIAEAWDAGGLYQVG FPHW IWSEWNG+Y
Sbjct: 453  ESDSFTTGTPLGSPPLIDMMSNDPILSKVKLIAEAWDAGGLYQVGKFPHWSIWSEWNGQY 512

Query: 1261 RDIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNK 1440
            RD VRQFIKGT+GFSGA AQCLCGSP+LYQ+GGRKPW+SINF+CAHDGFTLADLVTYNNK
Sbjct: 513  RDTVRQFIKGTNGFSGAFAQCLCGSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNK 572

Query: 1441 HNLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 1620
            +NL NGEDNRDGENHNLSWNCGEEG   SI VRRLRKRQMRNFFLCLMVSQGVPMIYMGD
Sbjct: 573  YNLSNGEDNRDGENHNLSWNCGEEGEFASITVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 632

Query: 1621 EYGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAE 1800
            EYGH+KGGNNN+YCHDN +NYF WD+KE++S DFYRFCRL+ KFR ECESLGL +FPTAE
Sbjct: 633  EYGHTKGGNNNTYCHDNYLNYFRWDRKEESSSDFYRFCRLMTKFRQECESLGLEDFPTAE 692

Query: 1801 RLKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLV 1980
            RL+WHG  P  PDWS+TSRFVAFS++DAVKGEIYVAFNTSHLPVT+ LP R GYRWEPLV
Sbjct: 693  RLQWHGRHPGMPDWSETSRFVAFSMTDAVKGEIYVAFNTSHLPVTITLPNRPGYRWEPLV 752

Query: 1981 DTSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRP 2124
            DT KP PYDFLS DLPDR  A+KQY++FL+ D YPMLSYSS++LVL P
Sbjct: 753  DTGKPTPYDFLSDDLPDRDTAVKQYAHFLNSDLYPMLSYSSIILVLHP 800


>ref|XP_010263294.1| PREDICTED: isoamylase 1, chloroplastic [Nelumbo nucifera]
          Length = 806

 Score = 1240 bits (3209), Expect = 0.0
 Identities = 560/709 (78%), Positives = 635/709 (89%)
 Frame = +1

Query: 1    SPVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHI 180
            SP+P GATA +GGVNFAIYS  AT+A LCL ++ DL+ N+VT+++ LDPL+NKTG++WH+
Sbjct: 95   SPMPFGATARDGGVNFAIYSGHATSATLCLISLSDLQENKVTEQICLDPLINKTGDVWHV 154

Query: 181  FLQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQM 360
            +L+G FEDM+YGY+FDGKFSPEEG Y+D SR+LLDPYAKAV+SRG+YG  G E  CWPQM
Sbjct: 155  YLKGDFEDMLYGYKFDGKFSPEEGHYYDSSRILLDPYAKAVISRGEYGALGPEGSCWPQM 214

Query: 361  AGMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHL 540
            AG+IP S ++FDW+GDLPL++PQ+DL+IYEMHVRGFT  +SS + +PGTY+G++EKLDHL
Sbjct: 215  AGIIPSSDNEFDWEGDLPLKHPQRDLIIYEMHVRGFTMHDSSKSRFPGTYIGVVEKLDHL 274

Query: 541  KDLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGR 720
            K+LG+NCIELMPC E+NELEYFSYN V    K+NFWGYST+NYFSPMIRYSS GI NCGR
Sbjct: 275  KELGINCIELMPCQEYNELEYFSYNSVLGDYKLNFWGYSTVNYFSPMIRYSSGGILNCGR 334

Query: 721  DAINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYN 900
            DAINE KTL+REAHKRGIEVLLDVVFNHTAEGNENGP+ SFRG+DNS YYM+APKGEFYN
Sbjct: 335  DAINEVKTLIREAHKRGIEVLLDVVFNHTAEGNENGPVLSFRGVDNSAYYMLAPKGEFYN 394

Query: 901  YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPI 1080
            YSGCGNTFNCNHPVVRQFI+DCLRYWVTEMHVDGFRFDLASI+TR  SLWD VN YG PI
Sbjct: 395  YSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASILTRGCSLWDAVNVYGNPI 454

Query: 1081 EGDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKY 1260
            EGD+LTTG  L NPPLI MISNDPILC VKLIAEAWD GGLYQVG+FPHW IWSEWNGKY
Sbjct: 455  EGDLLTTGTPLGNPPLIDMISNDPILCGVKLIAEAWDTGGLYQVGTFPHWGIWSEWNGKY 514

Query: 1261 RDIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNK 1440
            RDIVRQFIKGTDGFSG+ A+C+CGSP+LYQ+ GRKPW+SINFICAHDGFTLADLVTYNNK
Sbjct: 515  RDIVRQFIKGTDGFSGSFAECICGSPNLYQERGRKPWNSINFICAHDGFTLADLVTYNNK 574

Query: 1441 HNLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 1620
            HNL NGEDN DGENHN SWNCG+EG   S+ V+RLRKRQMRNFFLCLMVSQGVPMIYMGD
Sbjct: 575  HNLANGEDNNDGENHNNSWNCGQEGEFASLPVKRLRKRQMRNFFLCLMVSQGVPMIYMGD 634

Query: 1621 EYGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAE 1800
            EYGH+KGGNNN+YCHDN INYF WDKKE++S DF+RFCRL+  FR ECESLGL++FPTAE
Sbjct: 635  EYGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFFRFCRLMTTFRRECESLGLNDFPTAE 694

Query: 1801 RLKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLV 1980
            RL+WHGH P  PDWSDTSRFVAFSL DAVKGEIYVAFNTSHLPV V LPER GYRWEPLV
Sbjct: 695  RLQWHGHAPRMPDWSDTSRFVAFSLVDAVKGEIYVAFNTSHLPVMVTLPERPGYRWEPLV 754

Query: 1981 DTSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            DTSKP+P+DFLS DLP+R +AIKQYS+FLD + YPMLSYSS++L L PD
Sbjct: 755  DTSKPSPFDFLSEDLPERDVAIKQYSHFLDANLYPMLSYSSIILTLSPD 803


>ref|XP_020110388.1| isoamylase 1, chloroplastic isoform X1 [Ananas comosus]
          Length = 796

 Score = 1239 bits (3207), Expect = 0.0
 Identities = 572/709 (80%), Positives = 628/709 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P PLGATAC GGVNFAIYS +ATAAALCLFT+ DL+AN VT+ + LDP+VNKTGN WH+F
Sbjct: 90   PAPLGATACEGGVNFAIYSGSATAAALCLFTLADLKANNVTELIPLDPVVNKTGNTWHVF 149

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            LQG F+DM+YGY+FDGKFSPEEG YFDYSRV+LDPYAKA++ R  YGV G    CWPQMA
Sbjct: 150  LQGEFQDMLYGYKFDGKFSPEEGHYFDYSRVVLDPYAKAIIGREAYGVLGLSGSCWPQMA 209

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GMIP+  + FDW+GDLPL YPQKDLVIYEMHVRGFTN +SS T  PGTY G IEKLDHL 
Sbjct: 210  GMIPLLGNKFDWEGDLPLRYPQKDLVIYEMHVRGFTNHDSSKTDCPGTYAGAIEKLDHLT 269

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPC EFNELEYFS N V   NK+NFWGYSTINYFSPMIRYSS GI+NCGRD
Sbjct: 270  ELGVNCIELMPCQEFNELEYFSDNSVLGENKLNFWGYSTINYFSPMIRYSSTGISNCGRD 329

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK  VREAHKRGIEVLLDVVFNHTAEGNENGPI SFRGID++TYYM+APKGEFYNY
Sbjct: 330  AINEFKMFVREAHKRGIEVLLDVVFNHTAEGNENGPILSFRGIDSNTYYMLAPKGEFYNY 389

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQFIVDCLRYWV EMHVDGFRFDLASIMTRSSSLWDP+N YG  I 
Sbjct: 390  SGCGNTFNCNHPVVRQFIVDCLRYWVIEMHVDGFRFDLASIMTRSSSLWDPINVYGNSI- 448

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
             D++TTG  L NPPLI MISNDP+L  VKLIAEAWDAGGLYQVGSFPHW IWSEWNG+YR
Sbjct: 449  -DLITTGSPLGNPPLIDMISNDPVLNGVKLIAEAWDAGGLYQVGSFPHWGIWSEWNGQYR 507

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQF+KGTDGFSGA AQCLCGSP+LYQ+GGRKPWHSINF+CAHDGFTLADLVTYNNK+
Sbjct: 508  DIVRQFMKGTDGFSGAFAQCLCGSPNLYQEGGRKPWHSINFVCAHDGFTLADLVTYNNKY 567

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDNRDGENHNLSWNCGEEG   SI VRRLR+RQMRNFF+CL+VSQGVPMI+MGDE
Sbjct: 568  NLSNGEDNRDGENHNLSWNCGEEGEFASITVRRLRRRQMRNFFVCLLVSQGVPMIFMGDE 627

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE+A+ DFYRFCRL+  FR ECESLGL  FPTAER
Sbjct: 628  YGHTKGGNNNTYCHDNYINYFRWDKKEEAASDFYRFCRLMTNFRRECESLGLDNFPTAER 687

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGHQP  PDWS+TSRF+AFS++D VKGEIYVAFN +H PV V LPER GYRW+PLVD
Sbjct: 688  LQWHGHQPRMPDWSETSRFIAFSMTDKVKGEIYVAFNANHQPVIVTLPERPGYRWDPLVD 747

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPDV 2130
            TSK  PYDFLS DLPDRA AI+Q ++FL+ + YPMLSYSSV+L+LRPDV
Sbjct: 748  TSKAVPYDFLSNDLPDRASAIQQCAHFLNSNLYPMLSYSSVILILRPDV 796


>ref|XP_012075534.1| isoamylase 1, chloroplastic [Jatropha curcas]
 gb|KDP34860.1| hypothetical protein JCGZ_09148 [Jatropha curcas]
          Length = 793

 Score = 1223 bits (3164), Expect = 0.0
 Identities = 551/708 (77%), Positives = 629/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GATA + GVNF++YS+ A +A+LCL ++ DL  N+VT+E+ LDPL NKTG+IWH+F
Sbjct: 83   PAPFGATASDDGVNFSVYSTNAVSASLCLISLDDLPKNKVTEEIPLDPLTNKTGDIWHVF 142

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F+DM+YGYRFDGK SPEEG YF+ S ++LDPYAKAV+SRG++GV G +D+CWPQMA
Sbjct: 143  LKGDFKDMLYGYRFDGKLSPEEGHYFESSEIVLDPYAKAVISRGEFGVLGPDDNCWPQMA 202

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GMIP + D FDW+GDLPL+YPQKDL+IYEMHVRGFT  ESS T +PGTYLG++EKLDHLK
Sbjct: 203  GMIPAARDKFDWEGDLPLKYPQKDLMIYEMHVRGFTQHESSRTEFPGTYLGVVEKLDHLK 262

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEY+SYN V    KMNFWGYST+NYFSPM RYSS G  NCG D
Sbjct: 263  ELGVNCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTVNYFSPMTRYSSAGTRNCGHD 322

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK LVREAHKRGIEV++DVVFNHTAEGNE GPI SFRG+DNS YYM+APKGEFYNY
Sbjct: 323  AINEFKLLVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGVDNSVYYMLAPKGEFYNY 382

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHP+VRQFI+DCLRYWV EMHVDGFRFDLASIMTR SSLWD VN +G P+E
Sbjct: 383  SGCGNTFNCNHPIVRQFILDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPVE 442

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD+LTTG  L++PPLI MISNDPIL +VKLIAEAWDAGGLYQVG FPHW IWSEWNGKYR
Sbjct: 443  GDLLTTGTPLSSPPLIDMISNDPILREVKLIAEAWDAGGLYQVGCFPHWQIWSEWNGKYR 502

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGFSGA A+CLCGSP+LYQ+GGRKPW+SINF+CAHDGFTLADLVTYNNK+
Sbjct: 503  DIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNKN 562

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGENHN SWNCG EG   SI+V++LRKRQMRNFF+CLMVSQGVPMIYMGDE
Sbjct: 563  NLANGEDNNDGENHNNSWNCGLEGEFASISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDE 622

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE++S DFYRFC L+ KFR+ECESLGL++FPTAER
Sbjct: 623  YGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFYRFCCLMTKFRSECESLGLNDFPTAER 682

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWS+TSRFVAF+++D VKGEIYVAFN  HLPVTV LPER+GYRWEPLVD
Sbjct: 683  LQWHGHVPRMPDWSETSRFVAFTVTDTVKGEIYVAFNAKHLPVTVALPERLGYRWEPLVD 742

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            T K +P+DFLSGD+P+R IAIKQYS+FLD + YPM SYSS++L L PD
Sbjct: 743  TGKASPFDFLSGDVPEREIAIKQYSHFLDANLYPMHSYSSIILTLSPD 790


>gb|AUZ20772.1| isoamylase 1 (plastid) [Manihot esculenta]
          Length = 794

 Score = 1213 bits (3138), Expect = 0.0
 Identities = 549/708 (77%), Positives = 623/708 (87%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNF++YS+ A +A+LCL ++ DL  NRVT+E+ LDPL NKTG IWH+F
Sbjct: 84   PAPFGATVRDGGVNFSVYSANAVSASLCLISLDDLAENRVTEEIPLDPLANKTGYIWHVF 143

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F D++YGYRFDGKFS  EG YFD S+++LDPYAKAV+SRG++GV G +D+CWPQMA
Sbjct: 144  LKGEFRDVLYGYRFDGKFSRGEGHYFDSSKIVLDPYAKAVISRGEFGVLGPDDNCWPQMA 203

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GMIP + D FDWQGDLPL Y QKDLVIYEMHVRGFT  ESS T +PGTYLG++EKLDHLK
Sbjct: 204  GMIPAAQDKFDWQGDLPLRYSQKDLVIYEMHVRGFTRHESSRTEFPGTYLGIVEKLDHLK 263

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEYFSYN V   ++MNFWGYST+NYFSPM RYSS G  NCGRD
Sbjct: 264  ELGVNCIELMPCHEFNELEYFSYNSVLGDHRMNFWGYSTVNYFSPMTRYSSAGTRNCGRD 323

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK LVREAHKRGIEV++DVVFNHTAEGNE GPI SFRGIDNS YYM+APKGEFYNY
Sbjct: 324  AINEFKFLVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSVYYMLAPKGEFYNY 383

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQ I+DCLRYWV EMHVDGFRFDLASIMTR SSLWDPVN +G PIE
Sbjct: 384  SGCGNTFNCNHPVVRQLILDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDPVNVFGKPIE 443

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD+LTTG  L +PPLI MISNDPIL +VKLIAEAWDAGGLYQVG+FPHW IWSEWNGK+R
Sbjct: 444  GDLLTTGSPLGSPPLIDMISNDPILREVKLIAEAWDAGGLYQVGTFPHWQIWSEWNGKFR 503

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGF+GA A+CLCGSP+LYQ+GGRKPW+SINF+CAHDGFTLADLVTYNNK+
Sbjct: 504  DIVRQFIKGTDGFAGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNKN 563

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGENHN SWNCG+EG   SI V++LRKRQMRNFF+CLMVSQG+PMIYMGDE
Sbjct: 564  NLANGEDNNDGENHNNSWNCGQEGEFASILVKKLRKRQMRNFFVCLMVSQGIPMIYMGDE 623

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE++S DFYRFC  + KFR+EC+SLGL++FPTAER
Sbjct: 624  YGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFYRFCCQMTKFRHECKSLGLNDFPTAER 683

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWS+TSRFVAF+  D+ KGEIYVAFN +HLPVTV LPER GYRWEPLVD
Sbjct: 684  LQWHGHYPCVPDWSETSRFVAFTQIDSAKGEIYVAFNANHLPVTVTLPERPGYRWEPLVD 743

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            T KPAPYDFL  D+P+R  A+KQY +FLD +FYPM SYSS++L+L PD
Sbjct: 744  TGKPAPYDFLYSDVPERDAAVKQYGHFLDANFYPMHSYSSIILILSPD 791


>ref|XP_021614773.1| isoamylase 1, chloroplastic [Manihot esculenta]
 gb|OAY47124.1| hypothetical protein MANES_06G054000 [Manihot esculenta]
          Length = 794

 Score = 1212 bits (3135), Expect = 0.0
 Identities = 548/708 (77%), Positives = 623/708 (87%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNF++YS+ A +A+LCL ++ DL  NRVT+E+ LDPL NKTG IWH+F
Sbjct: 84   PAPFGATVRDGGVNFSVYSANAVSASLCLISLDDLAENRVTEEIPLDPLANKTGYIWHVF 143

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F D++YGYRFDGKFS  EG YFD S+++LDPYAKAV+SRG++GV G +D+CWPQMA
Sbjct: 144  LKGEFRDVLYGYRFDGKFSRGEGHYFDSSKIVLDPYAKAVISRGEFGVLGPDDNCWPQMA 203

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GMIP + D FDWQGDLPL Y QKDLVIYEMHVRGFT  ESS T +PGTYLG++EKLDHLK
Sbjct: 204  GMIPAAQDKFDWQGDLPLRYSQKDLVIYEMHVRGFTQHESSRTEFPGTYLGIVEKLDHLK 263

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEYFSYN V   ++MNFWGYST+NYFSPM RYSS G  NCGRD
Sbjct: 264  ELGVNCIELMPCHEFNELEYFSYNSVLGDHRMNFWGYSTVNYFSPMTRYSSAGTRNCGRD 323

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK LVREAHKRGIEV++DVVFNHTAEGNE GPI SFRGIDNS YYM+APKGEFYNY
Sbjct: 324  AINEFKFLVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSVYYMLAPKGEFYNY 383

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQ I+DCLRYWV EMHVDGFRFDLASIMTR SSLWDPVN +G PIE
Sbjct: 384  SGCGNTFNCNHPVVRQLILDCLRYWVIEMHVDGFRFDLASIMTRGSSLWDPVNVFGKPIE 443

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD+LTTG  L +PPLI MISNDPIL +VKLIAEAWDAGGLYQVG+FPHW IWSEWNGK+R
Sbjct: 444  GDLLTTGSPLGSPPLIDMISNDPILREVKLIAEAWDAGGLYQVGTFPHWQIWSEWNGKFR 503

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGF+GA A+CLCGSP+LYQ+GGRKPW+SINF+CAHDGFTLADLVTYNNK+
Sbjct: 504  DIVRQFIKGTDGFAGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNKN 563

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGENHN SWNCG+EG   SI V++LRKRQMRNFF+CLMVSQG+PMIYMGDE
Sbjct: 564  NLANGEDNNDGENHNNSWNCGQEGEFASILVKKLRKRQMRNFFVCLMVSQGIPMIYMGDE 623

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE++S DFYRFC  + KFR+EC+SLGL++FPTAER
Sbjct: 624  YGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFYRFCCQMTKFRHECKSLGLNDFPTAER 683

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWS+TSRFVAF+  D+ KGEIYVAFN +HLPVTV LPER GYRWEPLVD
Sbjct: 684  LQWHGHYPCVPDWSETSRFVAFTQIDSAKGEIYVAFNANHLPVTVTLPERPGYRWEPLVD 743

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            T KPAPYDFL  D+P+R  A+KQY +FLD +FYPM +YSS++L+L PD
Sbjct: 744  TGKPAPYDFLYSDVPERDAAVKQYGHFLDANFYPMHTYSSIILILSPD 791


>ref|XP_010048547.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Eucalyptus
            grandis]
 gb|KCW80824.1| hypothetical protein EUGRSUZ_C02189 [Eucalyptus grandis]
          Length = 794

 Score = 1211 bits (3133), Expect = 0.0
 Identities = 539/708 (76%), Positives = 630/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P PLGATAC GGVNF++YSS+A +A LCL ++ DL  +RVT+E+ LDP++N+TG++WH+F
Sbjct: 84   PTPLGATACVGGVNFSVYSSSAASATLCLISLADLNESRVTEEISLDPMINRTGDVWHVF 143

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F D++YGY+FDGK+SPEEG  +D S +LLDPYAKAV+SRG+YGV G + DCWPQMA
Sbjct: 144  LKGDFTDVLYGYKFDGKYSPEEGHRYDASHILLDPYAKAVISRGEYGVLGPDGDCWPQMA 203

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
             M+P   D+FDW+GDLPL+Y  KDL+IYEMHVRGFT  +SS T +PGTY G++EKLDHLK
Sbjct: 204  CMVPSDGDEFDWEGDLPLKYHPKDLIIYEMHVRGFTRHDSSKTDFPGTYRGVVEKLDHLK 263

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LG+NCIELMPCHEFNELEYFSYN V    KMNFWGYSTINYFSPM+RYSS G+++CG+D
Sbjct: 264  ELGINCIELMPCHEFNELEYFSYNTVLGDYKMNFWGYSTINYFSPMVRYSSAGLSSCGQD 323

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFKTL++EAHKRGIEVLLDVVFNHTAEGNENGP+ SFRGIDNS YYM+APKGEFYNY
Sbjct: 324  AINEFKTLIKEAHKRGIEVLLDVVFNHTAEGNENGPVLSFRGIDNSVYYMLAPKGEFYNY 383

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNT NCNHP VRQFIVDCLRYW TEMHVDGFRFDLASI+TRSSSLWD  N +G PIE
Sbjct: 384  SGCGNTLNCNHPAVRQFIVDCLRYWATEMHVDGFRFDLASILTRSSSLWDAANVFGKPIE 443

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD+LTTG  L++PPLI MISNDPIL  VKLIAEAWD GGLYQVG+FPHW IWSEWNGKYR
Sbjct: 444  GDLLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWDTGGLYQVGTFPHWGIWSEWNGKYR 503

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            D+VRQFIKGTDGFSGA A+ LCGSP+LYQ+GGR+PW+S+NF+CAHDGFTLADLV+YN+KH
Sbjct: 504  DVVRQFIKGTDGFSGAFAESLCGSPNLYQEGGRRPWNSVNFVCAHDGFTLADLVSYNDKH 563

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL+NGEDN DGE+HN SWNCG+EG    I+V+RLRKRQMRNFF+CLMVSQG+PM+YMGDE
Sbjct: 564  NLVNGEDNNDGESHNNSWNCGQEGEFAGISVKRLRKRQMRNFFVCLMVSQGIPMLYMGDE 623

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNNSYCHDN INYF WDKKE++S DFYRFC L+ KFR+ECESLGLS+FPTAER
Sbjct: 624  YGHTKGGNNNSYCHDNYINYFRWDKKEESSSDFYRFCSLMTKFRHECESLGLSDFPTAER 683

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P KPDWS+ SRFVA+++ D+V+GEIY+AFN SHLPVTV LPER GYRWEPLVD
Sbjct: 684  LQWHGHLPGKPDWSENSRFVAYTMVDSVRGEIYIAFNASHLPVTVVLPERPGYRWEPLVD 743

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAP+DFLS DLP+R +A+KQY+ FLD + YPMLSYSS++L L PD
Sbjct: 744  TSKPAPFDFLSSDLPERDVAVKQYAQFLDSNLYPMLSYSSIILSLSPD 791


>ref|XP_020265323.1| isoamylase 1, chloroplastic isoform X3 [Asparagus officinalis]
 gb|ONK68139.1| uncharacterized protein A4U43_C05F7850 [Asparagus officinalis]
          Length = 792

 Score = 1211 bits (3132), Expect = 0.0
 Identities = 549/709 (77%), Positives = 625/709 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT C+GGVNFAI+S  A++A LCLFTI DL+AN VT+E+ LDPL+NKTG +WHIF
Sbjct: 83   PAPFGATTCDGGVNFAIFSRGASSATLCLFTIADLKANIVTEEIPLDPLINKTGYVWHIF 142

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G  +DM+YGY+FDGKF PEEG YFD SRVLLDPYAKAV+SRG+YG  G + +CWPQMA
Sbjct: 143  LEGELKDMLYGYKFDGKFCPEEGHYFDKSRVLLDPYAKAVISRGEYGALGPDGNCWPQMA 202

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GMIP+S ++FDW+GDLPL YPQKDL+IYEMH+RG+T  +SS T +PGTYLG I+KLDHLK
Sbjct: 203  GMIPLSDNEFDWEGDLPLRYPQKDLIIYEMHIRGYTKHDSSKTDFPGTYLGAIDKLDHLK 262

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMP  EFNE+EYF+Y+ +   +K+NFWGYST+NYFSPM+RYSS G  NCGRD
Sbjct: 263  ELGVNCIELMPSQEFNEMEYFNYSSLPGESKLNFWGYSTVNYFSPMMRYSSSGETNCGRD 322

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINE K+LVREAHKRGIEV+LDVVFNHTAEGNENGPI SFRG+DNS YYM+APKGEFYNY
Sbjct: 323  AINEIKSLVREAHKRGIEVILDVVFNHTAEGNENGPILSFRGVDNSIYYMLAPKGEFYNY 382

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHP+VRQFI+DCLRYWV EMHVDGFRFDLASIMTR+SS W+  N YG P+E
Sbjct: 383  SGCGNTFNCNHPIVRQFILDCLRYWVIEMHVDGFRFDLASIMTRASSQWEAANVYGNPVE 442

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD LTTG  L++PPLI MISNDPIL  +KLIAEAWDAGGLYQVG FPHW  WSEWNG+YR
Sbjct: 443  GDTLTTGTPLSSPPLIDMISNDPILRGIKLIAEAWDAGGLYQVGQFPHWKTWSEWNGQYR 502

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGFSGA A+CLCGSP+LYQ GGRKPW+SINF+CAHDGFTLADLVTYNNK+
Sbjct: 503  DIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYNNKN 562

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDNRDGENHNLSWNCGEEG   S++VRRLR+RQMRNFF+CLMVSQGVPMIYMGDE
Sbjct: 563  NLSNGEDNRDGENHNLSWNCGEEGEFASMSVRRLRRRQMRNFFMCLMVSQGVPMIYMGDE 622

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNNSYCHDN INYF WDKKE++S D +RFCRL+ KFR ECE+LGL +FPTAER
Sbjct: 623  YGHTKGGNNNSYCHDNYINYFRWDKKEESSHDLFRFCRLMTKFRQECEALGLEDFPTAER 682

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGHQP  PDWS  SRFVAFSL D+VK EIY+AFNTSHLPV V LP+R GYRWEPLVD
Sbjct: 683  LQWHGHQPGMPDWSPESRFVAFSLIDSVKIEIYIAFNTSHLPVAVTLPDRPGYRWEPLVD 742

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPDV 2130
            TSKP PYDFL+ DL DR+  +KQ SNFLD + YPMLSYSS++L L+PDV
Sbjct: 743  TSKPPPYDFLTADLHDRSTVLKQQSNFLDSNLYPMLSYSSIILTLQPDV 791


>ref|XP_021277024.1| isoamylase 1, chloroplastic isoform X1 [Herrania umbratica]
          Length = 794

 Score = 1207 bits (3123), Expect = 0.0
 Identities = 544/708 (76%), Positives = 627/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNFAIYS+ A +A LCL T+ DL+ NRVT+++ LDPL NK G++WH+F
Sbjct: 84   PAPFGATLRDGGVNFAIYSANAVSATLCLITLSDLQQNRVTEQISLDPLTNKIGDVWHVF 143

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F+DM+YGY FDGKFSP++G Y+D S++LLDPYAKAV+SRG++G  G ED+CWPQMA
Sbjct: 144  LRGDFKDMLYGYIFDGKFSPKKGHYYDSSKILLDPYAKAVISRGEFGALGPEDNCWPQMA 203

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
             M+P S D+FDW+GDLPL Y Q+DL+IYEMHVRGFT  ESS T +PGTY G++EKLDHLK
Sbjct: 204  CMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTYHGVVEKLDHLK 263

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEY+SYN V    K+NFWGYST+NYFSPMIRYSS G+ NCGRD
Sbjct: 264  ELGVNCIELMPCHEFNELEYYSYNSVLGDYKLNFWGYSTVNYFSPMIRYSSSGMCNCGRD 323

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK LV+EAHKRGIEV +DVVFNHTAEGNENGPI SFRG+DNS YYM+APKGE+YNY
Sbjct: 324  AINEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPKGEYYNY 383

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQFI+DCLRYWVTEMHVDGFRFDLASIMTRSSSLWD VN +G P+E
Sbjct: 384  SGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDLVNVFGNPLE 443

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD++TTG  LN+PPLI M+SNDP+L  VKLIAEAWD GGLYQVGSFPHW IWSEWNGKYR
Sbjct: 444  GDLITTGTPLNSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHWGIWSEWNGKYR 503

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGFSGA A+CLCGSP+LYQ+GGRKPW+SINF+CAHDGF+LADLVTYNNKH
Sbjct: 504  DIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFSLADLVTYNNKH 563

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGE+HN SWNCG+EG    I+V++LRKRQMRNFFLCLMVSQG+PMI MGDE
Sbjct: 564  NLANGEDNNDGESHNNSWNCGQEGEFARISVKKLRKRQMRNFFLCLMVSQGIPMICMGDE 623

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKK+++S DF+RFC L+  FR ECESLGL++FPTAER
Sbjct: 624  YGHTKGGNNNTYCHDNYINYFRWDKKKESSSDFFRFCCLMTNFRRECESLGLNDFPTAER 683

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWSD SRFVAF+L D+VKGEIYVAFNTS+LPVT+ LPER GYRWEPLVD
Sbjct: 684  LQWHGHTPGMPDWSDKSRFVAFTLIDSVKGEIYVAFNTSYLPVTITLPERPGYRWEPLVD 743

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAP+DFLS +L +R IAIKQY+ FLD + YPMLSYSS++L+L PD
Sbjct: 744  TSKPAPFDFLSNELKERDIAIKQYAQFLDVNLYPMLSYSSIILLLSPD 791


>ref|XP_007014466.2| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Theobroma cacao]
          Length = 789

 Score = 1207 bits (3123), Expect = 0.0
 Identities = 543/708 (76%), Positives = 627/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNFAIYS+ A +A LCL T  DL+ NRVT+++ LDPL NKTG++WH+F
Sbjct: 79   PGPFGATLLDGGVNFAIYSANAVSATLCLITFSDLQQNRVTEQISLDPLTNKTGDVWHVF 138

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F+DM+YGY FDGKFSPE+G Y+D S++LLDPYAKAV+SRG++G  G ED+CWPQMA
Sbjct: 139  LRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAVISRGEFGALGPEDNCWPQMA 198

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
             M+P S D+FDW+GDLPL Y Q+DL+IYEMHVRGFT  ESS T +PGTY G++EKLDHLK
Sbjct: 199  CMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTYRGVVEKLDHLK 258

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEY+SYN +    K+NFWGYST+N+FSPMIRYSS G+ NCGRD
Sbjct: 259  ELGVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTVNFFSPMIRYSSSGMHNCGRD 318

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            A+NEFK LV+EAHKRGIEV +DVVFNHTAEGNENGPI SFRG+DNS YYM+APKGE+YNY
Sbjct: 319  AVNEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPKGEYYNY 378

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQFI+DCLRYWVTEMHVDGFRFDLASIMTRSSSLWD VN +G P++
Sbjct: 379  SGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDLVNVFGNPLK 438

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD++TTG  L++PPLI M+SNDP+L  VKLIAEAWD GGLYQVGSFPHW IWSEWNGKYR
Sbjct: 439  GDLITTGTPLSSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHWGIWSEWNGKYR 498

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGFSGA A+CLCGSP+LYQ+GGRKPW+S+NF+CAHDGFTLADLVTYNNKH
Sbjct: 499  DIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSVNFVCAHDGFTLADLVTYNNKH 558

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGE+HN SWNCG+EG   SI+V++LRKRQMRNFFLCLMVSQG+PMI MGDE
Sbjct: 559  NLANGEDNNDGESHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVSQGIPMICMGDE 618

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE+ S DF+RFC L+ KFR ECESLGL++FPTAER
Sbjct: 619  YGHTKGGNNNTYCHDNYINYFRWDKKEECSSDFFRFCCLMTKFRRECESLGLNDFPTAER 678

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWSD SRFVAF+L D+VKGEIYVAFNTS+LPVT+ LPER GYRWEPLVD
Sbjct: 679  LQWHGHTPGMPDWSDRSRFVAFTLIDSVKGEIYVAFNTSYLPVTITLPERPGYRWEPLVD 738

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAP+DFLS +L +R IAIKQY  FLD + YPMLSYS+++L+L PD
Sbjct: 739  TSKPAPFDFLSEELKERDIAIKQYVQFLDANLYPMLSYSTIILLLSPD 786


>gb|EOY32085.1| Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score = 1207 bits (3122), Expect = 0.0
 Identities = 544/708 (76%), Positives = 626/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNFAIYS+ A +A LCL T  DL+ NRVT+++ LDPL NKTG++WH+F
Sbjct: 85   PGPFGATLLDGGVNFAIYSANAVSATLCLITFSDLQQNRVTEQISLDPLTNKTGDVWHVF 144

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F+DM+YGY FDGKFSPE+G Y+D S++LLDPYAKAV+SRG++G  G ED+CWPQMA
Sbjct: 145  LRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAVISRGEFGALGPEDNCWPQMA 204

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
             M+P S D+FDW+GDLPL Y Q+DL+IYEMHVRGFT  ESS T +PGTY G++EKLDHLK
Sbjct: 205  CMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHESSRTEFPGTYRGVVEKLDHLK 264

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEY+SYN +    K+NFWGYST+N+FSPMIRYSS G+ NCGRD
Sbjct: 265  ELGVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTVNFFSPMIRYSSSGMHNCGRD 324

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            A+NEFK LV+EAHKRGIEV +DVVFNHTAEGNENGPI SFRG+DNS YYM+APKGE+YNY
Sbjct: 325  AVNEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSFRGVDNSVYYMLAPKGEYYNY 384

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQFI+DCLRYWVTEMHVDGFRFDLASIMTRSSSLWD VN +G P++
Sbjct: 385  SGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDLVNVFGNPLK 444

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD++TTG  L++PPLI M+SNDP+L  VKLIAEAWD GGLYQVGSFPHW IWSEWNGKYR
Sbjct: 445  GDLITTGTPLSSPPLIDMMSNDPVLRGVKLIAEAWDTGGLYQVGSFPHWGIWSEWNGKYR 504

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGFSGA A+CLCGSP+LYQ+GGRKPW+SINF+CAHDGFTLADLVTYNNKH
Sbjct: 505  DIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSINFVCAHDGFTLADLVTYNNKH 564

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGE+HN SWNCG+EG   SI+V++LRKRQMRNFFLCLMVSQG+PMI MGDE
Sbjct: 565  NLANGEDNNDGESHNNSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVSQGIPMICMGDE 624

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE+ S DF+RFC L+ KFR ECESLGL++FPTAER
Sbjct: 625  YGHTKGGNNNTYCHDNYINYFQWDKKEECSSDFFRFCCLMTKFRRECESLGLNDFPTAER 684

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWSD SRFVAF+L D+VKGEIYVAFNTS+LPVT+ LPE  GYRWEPLVD
Sbjct: 685  LQWHGHTPGMPDWSDRSRFVAFTLIDSVKGEIYVAFNTSYLPVTITLPEHPGYRWEPLVD 744

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAP+DFLS +L +R IAIKQY  FLD + YPMLSYSS++L+L PD
Sbjct: 745  TSKPAPFDFLSEELKERDIAIKQYVQFLDANLYPMLSYSSIILLLSPD 792


>ref|XP_022134724.1| isoamylase 1, chloroplastic [Momordica charantia]
          Length = 808

 Score = 1206 bits (3120), Expect = 0.0
 Identities = 543/707 (76%), Positives = 628/707 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P PLGATA +GG+NFA+YS  A +A LCL  + DL+ NRVTKEV LDPL NKTG++WH+F
Sbjct: 97   PAPLGATARDGGMNFAVYSGNAVSATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVF 156

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F +M+YGY+FDG+FSP++G YFD SR+LLDPYAKAV+SRG++G PG +++CWPQMA
Sbjct: 157  LKGGFTEMLYGYKFDGEFSPQDGCYFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMA 216

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GM+P   D+FDW+GDLPL+YPQKDL+IYEMHVRGFT  ESS + +PGTYLGL+EKLD+LK
Sbjct: 217  GMVPTVIDEFDWEGDLPLQYPQKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLK 276

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEYFSYN V    KMNFWGYST+ +FSPMIRYSS GI  CG+D
Sbjct: 277  ELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQD 336

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK L+REAHKRGIEV++DVVFNHTAEGNENGPIFSFRG+DNS YYM+AP GEFYNY
Sbjct: 337  AINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNY 396

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHP VR+FIVDCLRYWVTEMHVDGFRFDLASIMTR SSLWD VN YG PIE
Sbjct: 397  SGCGNTFNCNHPAVRRFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIE 456

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD+LTTG  L NPPLI MISNDP+L  VKLIAEAWDAGGLYQVG+FPHW +WSEWNG+YR
Sbjct: 457  GDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGQYR 516

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDGFSGA A+CLCGSP+LYQ GGRKPW+SINFICAHDGFTLADLVTYNNKH
Sbjct: 517  DIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNNKH 576

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGE+HN SWNCG+EG   S++V++LRKRQMRNFF+CLMVSQGVPMI+MGDE
Sbjct: 577  NLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQMRNFFVCLMVSQGVPMIHMGDE 636

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDK E++S DF+RFC L+  FR+ECESLGL++FPTAER
Sbjct: 637  YGHTKGGNNNTYCHDNYINYFRWDKLEESSSDFHRFCSLVTTFRHECESLGLNDFPTAER 696

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHG+ P KPDWS+ SRFVAF+L D+VKGEIY+AFN +HLPVTV LPER GYRWEPLVD
Sbjct: 697  LQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNANHLPVTVTLPERPGYRWEPLVD 756

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRP 2124
            T K +P+DFLS DLP+R +AI+QY++FLD + YPML YSS++L+L P
Sbjct: 757  TGKISPFDFLSSDLPERDVAIRQYAHFLDNNLYPMLCYSSIILLLSP 803


>ref|XP_014510281.1| isoamylase 1, chloroplastic [Vigna radiata var. radiata]
          Length = 790

 Score = 1206 bits (3119), Expect = 0.0
 Identities = 547/708 (77%), Positives = 621/708 (87%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNFAIYS  A +A LCLFT+ D + NRVT+ V LDPL+NKTG +WH+F
Sbjct: 80   PSPFGATVRDGGVNFAIYSLNALSATLCLFTLSDFQNNRVTESVPLDPLINKTGAVWHVF 139

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F DM+YGY+FDGKFSP EGQY+D SR+LLDPYAKAV+SRG++G  G   +CWPQMA
Sbjct: 140  LKGDFTDMLYGYKFDGKFSPLEGQYYDPSRILLDPYAKAVISRGEFGALGPNGNCWPQMA 199

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GM+P   D+FDW+GDLPL+YPQKDLV+YEMHVRGFT  ESS T +PGTYLG++EKLDHLK
Sbjct: 200  GMVPSDHDEFDWEGDLPLKYPQKDLVVYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLK 259

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNC+ELMPCHEFNELEY SYN V    ++NFWGYST+N+FSPMIRYSS GI NCGRD
Sbjct: 260  ELGVNCLELMPCHEFNELEYSSYNSVQGDYRVNFWGYSTVNFFSPMIRYSSAGIRNCGRD 319

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
             INE K L++EAHKRGIEV++DVVFNHTAEGNENGPI SFRG+DNS YYMVAPKGEFYNY
Sbjct: 320  GINEMKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPIISFRGVDNSIYYMVAPKGEFYNY 379

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNC+HPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWD  N +G PIE
Sbjct: 380  SGCGNTFNCSHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDATNVFGAPIE 439

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD+LTTG  L +PPLI +ISNDPIL  VKLIAEAWDAGGLYQVG+FPHW IWSEWNGKYR
Sbjct: 440  GDLLTTGTPLGSPPLIDLISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYR 499

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            D VRQFIKGTDGF+GA A+CLCGSP+LYQ GGRKPW+SINF+CAHDGFTLADLVTYNNKH
Sbjct: 500  DTVRQFIKGTDGFAGAFAECLCGSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTYNNKH 559

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGENHN SWNCG+EG   S  V++LRKRQMRNFFL LMVSQGVPMIYMGDE
Sbjct: 560  NLSNGEDNNDGENHNNSWNCGQEGEFVSPLVKKLRKRQMRNFFLSLMVSQGVPMIYMGDE 619

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN +NYF WDKKE++S DF+RFC L+ KFR ECES+GL +FPT+ER
Sbjct: 620  YGHTKGGNNNTYCHDNYLNYFQWDKKEESSSDFFRFCCLVTKFRQECESMGLDDFPTSER 679

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P  PDWS+TSRFVAF+++D+VKGEIYVAFNTSHLP T+ LPER GYRWEPL+D
Sbjct: 680  LQWHGHFPGMPDWSETSRFVAFTMADSVKGEIYVAFNTSHLPFTITLPERPGYRWEPLID 739

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAPYDFL+ DLP R IAI+QY+ FLD + YPMLSYSSV+L+  PD
Sbjct: 740  TSKPAPYDFLTPDLPGREIAIQQYAQFLDANMYPMLSYSSVILLRTPD 787


>ref|XP_020265321.1| isoamylase 1, chloroplastic isoform X1 [Asparagus officinalis]
          Length = 796

 Score = 1205 bits (3117), Expect = 0.0
 Identities = 549/713 (76%), Positives = 625/713 (87%), Gaps = 4/713 (0%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT C+GGVNFAI+S  A++A LCLFTI DL+AN VT+E+ LDPL+NKTG +WHIF
Sbjct: 83   PAPFGATTCDGGVNFAIFSRGASSATLCLFTIADLKANIVTEEIPLDPLINKTGYVWHIF 142

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G  +DM+YGY+FDGKF PEEG YFD SRVLLDPYAKAV+SRG+YG  G + +CWPQMA
Sbjct: 143  LEGELKDMLYGYKFDGKFCPEEGHYFDKSRVLLDPYAKAVISRGEYGALGPDGNCWPQMA 202

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GMIP+S ++FDW+GDLPL YPQKDL+IYEMH+RG+T  +SS T +PGTYLG I+KLDHLK
Sbjct: 203  GMIPLSDNEFDWEGDLPLRYPQKDLIIYEMHIRGYTKHDSSKTDFPGTYLGAIDKLDHLK 262

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMP  EFNE+EYF+Y+ +   +K+NFWGYST+NYFSPM+RYSS G  NCGRD
Sbjct: 263  ELGVNCIELMPSQEFNEMEYFNYSSLPGESKLNFWGYSTVNYFSPMMRYSSSGETNCGRD 322

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPK----GE 891
            AINE K+LVREAHKRGIEV+LDVVFNHTAEGNENGPI SFRG+DNS YYM+APK    GE
Sbjct: 323  AINEIKSLVREAHKRGIEVILDVVFNHTAEGNENGPILSFRGVDNSIYYMLAPKVKIEGE 382

Query: 892  FYNYSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYG 1071
            FYNYSGCGNTFNCNHP+VRQFI+DCLRYWV EMHVDGFRFDLASIMTR+SS W+  N YG
Sbjct: 383  FYNYSGCGNTFNCNHPIVRQFILDCLRYWVIEMHVDGFRFDLASIMTRASSQWEAANVYG 442

Query: 1072 IPIEGDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWN 1251
             P+EGD LTTG  L++PPLI MISNDPIL  +KLIAEAWDAGGLYQVG FPHW  WSEWN
Sbjct: 443  NPVEGDTLTTGTPLSSPPLIDMISNDPILRGIKLIAEAWDAGGLYQVGQFPHWKTWSEWN 502

Query: 1252 GKYRDIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTY 1431
            G+YRDIVRQFIKGTDGFSGA A+CLCGSP+LYQ GGRKPW+SINF+CAHDGFTLADLVTY
Sbjct: 503  GQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFVCAHDGFTLADLVTY 562

Query: 1432 NNKHNLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIY 1611
            NNK+NL NGEDNRDGENHNLSWNCGEEG   S++VRRLR+RQMRNFF+CLMVSQGVPMIY
Sbjct: 563  NNKNNLSNGEDNRDGENHNLSWNCGEEGEFASMSVRRLRRRQMRNFFMCLMVSQGVPMIY 622

Query: 1612 MGDEYGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFP 1791
            MGDEYGH+KGGNNNSYCHDN INYF WDKKE++S D +RFCRL+ KFR ECE+LGL +FP
Sbjct: 623  MGDEYGHTKGGNNNSYCHDNYINYFRWDKKEESSHDLFRFCRLMTKFRQECEALGLEDFP 682

Query: 1792 TAERLKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWE 1971
            TAERL+WHGHQP  PDWS  SRFVAFSL D+VK EIY+AFNTSHLPV V LP+R GYRWE
Sbjct: 683  TAERLQWHGHQPGMPDWSPESRFVAFSLIDSVKIEIYIAFNTSHLPVAVTLPDRPGYRWE 742

Query: 1972 PLVDTSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPDV 2130
            PLVDTSKP PYDFL+ DL DR+  +KQ SNFLD + YPMLSYSS++L L+PDV
Sbjct: 743  PLVDTSKPPPYDFLTADLHDRSTVLKQQSNFLDSNLYPMLSYSSIILTLQPDV 795


>ref|XP_020215619.1| isoamylase 1, chloroplastic [Cajanus cajan]
          Length = 791

 Score = 1204 bits (3114), Expect = 0.0
 Identities = 543/706 (76%), Positives = 621/706 (87%)
 Frame = +1

Query: 10   PLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIFLQ 189
            P GAT  +GGVNFAIYS  A +A LCLFT+ D + +RVT+ V LDPL+N+TG +WH+FL+
Sbjct: 83   PFGATVRDGGVNFAIYSLNALSATLCLFTLSDFQNDRVTEYVPLDPLINRTGGVWHVFLK 142

Query: 190  GPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMAGM 369
            G F DM+YGY+FDGKFSP EG Y+D SR++LDPYAKAV+SRG++G  G + +CWPQMAGM
Sbjct: 143  GDFRDMLYGYKFDGKFSPLEGHYYDPSRIVLDPYAKAVISRGEFGALGPDANCWPQMAGM 202

Query: 370  IPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLKDL 549
            +P   D+FDW+GDLPL+YPQ+DLVIYEMHVRGFT  ESS T +PGTYLG++EKLDHLK+L
Sbjct: 203  VPSDDDEFDWEGDLPLKYPQRDLVIYEMHVRGFTKHESSNTKFPGTYLGVVEKLDHLKEL 262

Query: 550  GVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRDAI 729
            GVNC+ELMPCHEFNELEYFSYN V    ++NFWGYSTINYFSPMIRYSS GI NCG D I
Sbjct: 263  GVNCLELMPCHEFNELEYFSYNSVQGDYRVNFWGYSTINYFSPMIRYSSAGIRNCGHDGI 322

Query: 730  NEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNYSG 909
            NE K L++EAHKRGIEV++DVVFNHTAEGNENGPI SFRG+DNS YYM+APKGEFYNYSG
Sbjct: 323  NEIKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPIISFRGVDNSVYYMLAPKGEFYNYSG 382

Query: 910  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIEGD 1089
            CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIM+RSSSLWD  N +G  IEGD
Sbjct: 383  CGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMSRSSSLWDGANVFGARIEGD 442

Query: 1090 MLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYRDI 1269
            +LTTG  L+ PPLI +ISNDPIL  VKLIAEAWDAGGLYQVG+FPHW IWSEWNGKYRD 
Sbjct: 443  LLTTGTPLSGPPLIDLISNDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRDT 502

Query: 1270 VRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKHNL 1449
            VRQFIKGTDGF+GA+A+CLCGSP+LYQ GGRKPWHSINF+CAHDGFTLADLVTYN+KHNL
Sbjct: 503  VRQFIKGTDGFAGALAECLCGSPNLYQGGGRKPWHSINFVCAHDGFTLADLVTYNSKHNL 562

Query: 1450 LNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDEYG 1629
             NGEDN DGENHN SWNCG+EG   S +V++LRKRQMRNFFL LMVSQGVPMIYMGDEYG
Sbjct: 563  SNGEDNNDGENHNNSWNCGQEGEFASTSVKKLRKRQMRNFFLSLMVSQGVPMIYMGDEYG 622

Query: 1630 HSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAERLK 1809
            H+KGGNNN+YCHDN INYF WDKKE++S DF+RFCRL+ KFR ECESLGL +FPT+ERL+
Sbjct: 623  HTKGGNNNTYCHDNYINYFRWDKKEESSSDFFRFCRLMTKFRQECESLGLDDFPTSERLQ 682

Query: 1810 WHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVDTS 1989
            WHGH P +PDWS+TSRFVAF++ D+VKGEIY+AFNTSHLP T+ LPER GYRWEPLVDTS
Sbjct: 683  WHGHFPGRPDWSETSRFVAFTMVDSVKGEIYIAFNTSHLPCTITLPERPGYRWEPLVDTS 742

Query: 1990 KPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            KP PYDFL+ DLP R IAI+QY+ FLD + YPMLSYSS++L+  PD
Sbjct: 743  KPTPYDFLTPDLPGRDIAIQQYAQFLDANMYPMLSYSSIILLRTPD 788


>gb|PIA43915.1| hypothetical protein AQUCO_01800158v1 [Aquilegia coerulea]
          Length = 829

 Score = 1202 bits (3111), Expect = 0.0
 Identities = 551/709 (77%), Positives = 628/709 (88%)
 Frame = +1

Query: 1    SPVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHI 180
            +P+P GATA +GGVNFA++SS A +A LCL ++ DL+ NRVTK++ LDPL+NKTG++WH 
Sbjct: 118  NPMPFGATAKDGGVNFAVHSSGAVSATLCLISLSDLQENRVTKKIHLDPLLNKTGDVWHA 177

Query: 181  FLQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQM 360
            FL+G FE+M+YGY FDGKFSPEEG  +D SR+LLDPYAKAVVSRG++G  G E++CWPQM
Sbjct: 178  FLKGDFENMLYGYMFDGKFSPEEGHCYDSSRILLDPYAKAVVSRGEFGALGPEENCWPQM 237

Query: 361  AGMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHL 540
            AGM+P SSD+FDW+GDLPL  PQ+DLVIYEMHVRGFT  +SS +++PGTYLG++EKLDHL
Sbjct: 238  AGMVPASSDEFDWEGDLPLMNPQRDLVIYEMHVRGFTRHDSSRSNFPGTYLGVVEKLDHL 297

Query: 541  KDLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGR 720
            K+LGVNCIELMP  E+NELEYFSYN V    K+NFWGYSTINYFSPM+RYSS GI NCG+
Sbjct: 298  KELGVNCIELMPFQEYNELEYFSYNSVLGNYKLNFWGYSTINYFSPMLRYSSAGIRNCGQ 357

Query: 721  DAINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYN 900
            DAINE K LVREAHKRGIEVL+DVVFNHTAEGNE GP  SFRG+DNS YYM+APKGE+YN
Sbjct: 358  DAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPTLSFRGVDNSVYYMLAPKGEYYN 417

Query: 901  YSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPI 1080
            YSGCGNTFNCNHPVVRQFIVDCLRYWV EMHVDGFRFDLA+I+TRSSSLWD VN YG  I
Sbjct: 418  YSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLAAILTRSSSLWDAVNVYGNSI 477

Query: 1081 EGDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKY 1260
            EGD LTTG  L +PPLI MISNDPIL +VKLIAEAWD GGLYQVG FPHW IWSEWNGKY
Sbjct: 478  EGDSLTTGTPLGSPPLIDMISNDPILRRVKLIAEAWDTGGLYQVGMFPHWGIWSEWNGKY 537

Query: 1261 RDIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNK 1440
            RDIVRQFIKGTDGFSGA A+CLCGSP+LYQ GGRKPW+SINF+CAHDGFTLADLVTYNNK
Sbjct: 538  RDIVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWNSINFVCAHDGFTLADLVTYNNK 596

Query: 1441 HNLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGD 1620
            HNL NGEDN DGE HN SWNCG+EG   S++V+RLRKRQMRNFFLCLMVSQGVPMIYMGD
Sbjct: 597  HNLANGEDNNDGETHNNSWNCGQEGEFASLSVKRLRKRQMRNFFLCLMVSQGVPMIYMGD 656

Query: 1621 EYGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAE 1800
            EYGH+KGGNNN+YCHDN INYF WDKKE++S DF+RFC L+ KFR ECESLGL +FPTA+
Sbjct: 657  EYGHTKGGNNNTYCHDNYINYFRWDKKEESSNDFFRFCCLMTKFRQECESLGLDDFPTAD 716

Query: 1801 RLKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLV 1980
            RL+WHGH P  PDWS+TSRFVAFSL D+VKGEIY+AFNTSHLPVTV LPER GYRWEPLV
Sbjct: 717  RLQWHGHSPGVPDWSETSRFVAFSLIDSVKGEIYLAFNTSHLPVTVSLPERPGYRWEPLV 776

Query: 1981 DTSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            DTSKPAP+DFLS D+P++A AI Q+S+FLD + YPMLSYSS++L+L P+
Sbjct: 777  DTSKPAPFDFLSDDVPEKATAITQFSHFLDANMYPMLSYSSIILLLTPE 825


>ref|XP_017615133.1| PREDICTED: isoamylase 1, chloroplastic [Gossypium arboreum]
          Length = 789

 Score = 1202 bits (3110), Expect = 0.0
 Identities = 543/708 (76%), Positives = 625/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  + GVNFAIYS+ AT+A LCL  + DL+ NR T+++ LDPL NKTG++WH+F
Sbjct: 79   PAPFGATIQDCGVNFAIYSANATSATLCLIALSDLQQNRATEQIPLDPLANKTGDVWHVF 138

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F+DM+YGY FDG+FSPE G Y+D SR+LLDPYAKAV+SRG++G  G ED+CWPQMA
Sbjct: 139  LKGNFKDMLYGYIFDGEFSPEIGHYYDSSRILLDPYAKAVISRGEFGALGPEDNCWPQMA 198

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GM+P S   FDW+GDLPL +P +DL+IYEMHVRG+T  ESSGT +PGTY G++EKLDHLK
Sbjct: 199  GMVPTSEYQFDWEGDLPLRHPDRDLIIYEMHVRGYTKHESSGTKFPGTYRGVVEKLDHLK 258

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPC EFNELEY+SYN V    K+NFWGYSTINYFSPMIRYSS GI +CGRD
Sbjct: 259  ELGVNCIELMPCQEFNELEYYSYNSVLGDYKLNFWGYSTINYFSPMIRYSSSGIRSCGRD 318

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK LV+EAHKRGIEV++DVVFNHTAEGNE G   SFRG+DN  YYM+APKGE+YNY
Sbjct: 319  AINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEKGLSLSFRGVDNCVYYMLAPKGEYYNY 378

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQFI+DCLRYWVTEMHVDGFRFDLASIMTRSSSLWD VN YG P+E
Sbjct: 379  SGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDSVNVYGDPLE 438

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD++TTG  L+NPPLI M+SNDP+L  VKLIAEAWDAGGLYQVG FPHW +WSEWNGKYR
Sbjct: 439  GDLITTGTPLSNPPLIDMMSNDPVLHGVKLIAEAWDAGGLYQVGRFPHWGVWSEWNGKYR 498

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDG SGA A+CLCGSP+LYQ+GG KPW+S+NFICAHDGFTLADLVTYNNKH
Sbjct: 499  DIVRQFIKGTDGLSGAFAECLCGSPNLYQEGGGKPWNSVNFICAHDGFTLADLVTYNNKH 558

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGE+HN SWNCG+EG   SI+V++LRKRQMRNFFLCLMVSQGVPMI+MGDE
Sbjct: 559  NLANGEDNNDGESHNYSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVSQGVPMIHMGDE 618

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE++S D +RF RL+ KFR ECESLGL++FPTAER
Sbjct: 619  YGHTKGGNNNTYCHDNYINYFRWDKKEESSSDLFRFFRLMTKFRRECESLGLNDFPTAER 678

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P KPDWSDTSRFVAF+L D+VKGEIYVAFNTSHLPVT+ LPER GYRWEPLVD
Sbjct: 679  LQWHGHTPGKPDWSDTSRFVAFTLIDSVKGEIYVAFNTSHLPVTITLPERPGYRWEPLVD 738

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAP+DFLS ++P+R +AIKQY++FLD + YPMLSYSS++L+L PD
Sbjct: 739  TSKPAPFDFLSNEVPERELAIKQYAHFLDANLYPMLSYSSIVLLLSPD 786


>ref|XP_016719009.1| PREDICTED: isoamylase 1, chloroplastic-like [Gossypium hirsutum]
          Length = 789

 Score = 1202 bits (3110), Expect = 0.0
 Identities = 542/708 (76%), Positives = 626/708 (88%)
 Frame = +1

Query: 4    PVPLGATACNGGVNFAIYSSTATAAALCLFTIPDLEANRVTKEVVLDPLVNKTGNIWHIF 183
            P P GAT  +GGVNFAIYS+ AT+A LCL ++ DL+ NRVT+++ LDPL NKTG++WH+F
Sbjct: 79   PAPFGATIQDGGVNFAIYSANATSATLCLISLSDLQQNRVTEQIPLDPLANKTGDVWHVF 138

Query: 184  LQGPFEDMVYGYRFDGKFSPEEGQYFDYSRVLLDPYAKAVVSRGQYGVPGQEDDCWPQMA 363
            L+G F+DM+YGY FDG+FSPE G Y+D SR+LLDPYAKAV+SRG++G  G ED+CWPQMA
Sbjct: 139  LKGNFKDMLYGYIFDGEFSPEIGHYYDSSRILLDPYAKAVISRGEFGALGPEDNCWPQMA 198

Query: 364  GMIPVSSDDFDWQGDLPLEYPQKDLVIYEMHVRGFTNDESSGTHYPGTYLGLIEKLDHLK 543
            GM+P S D FDW+GDLPL +P +DL+IYEMHVRG+T  ESSGT +PGTY G++EKLDHLK
Sbjct: 199  GMVPTSEDQFDWEGDLPLRHPGRDLIIYEMHVRGYTRHESSGTKFPGTYCGVVEKLDHLK 258

Query: 544  DLGVNCIELMPCHEFNELEYFSYNPVTEMNKMNFWGYSTINYFSPMIRYSSVGIANCGRD 723
            +LGVNCIELMPCHEFNELEY+SYN V    K+NFWGYSTINYFSPMIRYSS GI +CGRD
Sbjct: 259  ELGVNCIELMPCHEFNELEYYSYNSVLGDYKLNFWGYSTINYFSPMIRYSSSGIRSCGRD 318

Query: 724  AINEFKTLVREAHKRGIEVLLDVVFNHTAEGNENGPIFSFRGIDNSTYYMVAPKGEFYNY 903
            AINEFK LV+EAHKRGIEV++DVVFNHTAEGNE G   SFRG+DN  YYM+APKGE+YNY
Sbjct: 319  AINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEKGLSVSFRGVDNCVYYMLAPKGEYYNY 378

Query: 904  SGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASIMTRSSSLWDPVNAYGIPIE 1083
            SGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDLASIMTRSSSLWD VN YG P+E
Sbjct: 379  SGCGNTFNCNHPVVRQFILDCLRYWVAEMHVDGFRFDLASIMTRSSSLWDSVNVYGDPLE 438

Query: 1084 GDMLTTGKGLNNPPLIGMISNDPILCKVKLIAEAWDAGGLYQVGSFPHWHIWSEWNGKYR 1263
            GD++TTG  L+NPPLI M+SNDP+L  VKLIAEAWDAGGLYQVG FPHW +WSEWNGKYR
Sbjct: 439  GDLITTGTPLSNPPLIDMMSNDPVLHGVKLIAEAWDAGGLYQVGRFPHWGVWSEWNGKYR 498

Query: 1264 DIVRQFIKGTDGFSGAVAQCLCGSPDLYQDGGRKPWHSINFICAHDGFTLADLVTYNNKH 1443
            DIVRQFIKGTDG SGA A+CLCGSP+LYQ GG KPW+S+NFICAHDGFTLADLVTYNNKH
Sbjct: 499  DIVRQFIKGTDGLSGAFAECLCGSPNLYQKGGGKPWNSVNFICAHDGFTLADLVTYNNKH 558

Query: 1444 NLLNGEDNRDGENHNLSWNCGEEGNSGSIAVRRLRKRQMRNFFLCLMVSQGVPMIYMGDE 1623
            NL NGEDN DGE+HN SWNCG+EG   SI+V++LRKRQMRNFFLCLMVSQGVPMI+MGDE
Sbjct: 559  NLANGEDNNDGESHNYSWNCGQEGEFASISVKKLRKRQMRNFFLCLMVSQGVPMIHMGDE 618

Query: 1624 YGHSKGGNNNSYCHDNLINYFCWDKKEQASLDFYRFCRLLIKFRNECESLGLSEFPTAER 1803
            YGH+KGGNNN+YCHDN INYF WDKKE+++ D +RF  L+  FR ECESLGL++FPTAER
Sbjct: 619  YGHTKGGNNNTYCHDNYINYFRWDKKEESASDLFRFFCLMTMFRRECESLGLNDFPTAER 678

Query: 1804 LKWHGHQPHKPDWSDTSRFVAFSLSDAVKGEIYVAFNTSHLPVTVCLPERIGYRWEPLVD 1983
            L+WHGH P KPDWSDTSRFVAF+L D+VKGEIYVAFNTSHLPVT+ LP+R GYRWEPLVD
Sbjct: 679  LQWHGHTPGKPDWSDTSRFVAFTLIDSVKGEIYVAFNTSHLPVTITLPDRPGYRWEPLVD 738

Query: 1984 TSKPAPYDFLSGDLPDRAIAIKQYSNFLDCDFYPMLSYSSVLLVLRPD 2127
            TSKPAP+DFLS ++P+R +AIKQY++FLD + YPMLSYSS++L+L PD
Sbjct: 739  TSKPAPFDFLSNEVPERDLAIKQYAHFLDANLYPMLSYSSIVLLLSPD 786