BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cheilocostus21_contig00027081
         (1510 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_009393949.1| PREDICTED: E3 ubiquitin-protein ligase PUB22...   511   e-176
ref|XP_008796330.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   496   e-170
ref|XP_010911082.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   486   e-166
ref|XP_020096550.1| E3 ubiquitin-protein ligase PUB23-like [Anan...   457   e-155
gb|OVA01288.1| U box domain [Macleaya cordata]                        449   e-152
ref|XP_010275264.2| PREDICTED: E3 ubiquitin-protein ligase PUB22...   445   e-150
gb|PIA32753.1| hypothetical protein AQUCO_04400152v1 [Aquilegia ...   429   e-144
ref|XP_018728284.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   426   e-143
ref|XP_010269983.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   424   e-142
ref|XP_018845148.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   418   e-140
gb|ONI22128.1| hypothetical protein PRUPE_2G109000 [Prunus persica]   419   e-139
ref|XP_007219082.2| E3 ubiquitin-protein ligase PUB23 [Prunus pe...   417   e-139
ref|XP_008232231.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   417   e-139
ref|XP_004308738.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   414   e-138
ref|XP_024169270.1| E3 ubiquitin-protein ligase PUB23-like [Rosa...   410   e-136
ref|XP_022743887.1| E3 ubiquitin-protein ligase PUB23-like [Duri...   409   e-136
gb|PKA53966.1| E3 ubiquitin-protein ligase PUB23 [Apostasia shen...   409   e-136
ref|XP_008346772.1| PREDICTED: E3 ubiquitin-protein ligase PUB23...   409   e-136
gb|PRQ56801.1| putative aminoacyltransferase, E1 ubiquitin-activ...   410   e-136
ref|XP_002264927.3| PREDICTED: E3 ubiquitin-protein ligase PUB23...   408   e-136

>ref|XP_009393949.1| PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Musa acuminata
            subsp. malaccensis]
          Length = 408

 Score =  511 bits (1317), Expect = e-176
 Identities = 273/414 (65%), Positives = 329/414 (79%), Gaps = 8/414 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            MEA P LF+CPIS+ELMEDPVTIATGVTYERKNIEKWLFTYKK TCPATMQRLE+FDLTP
Sbjct: 1    MEA-PFLFQCPISLELMEDPVTIATGVTYERKNIEKWLFTYKKLTCPATMQRLESFDLTP 59

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            NHT+KRLISSF +   D++                 + ++L SLL+ +QTGPFKAS LRK
Sbjct: 60   NHTVKRLISSFLEHAADEE-----PVTSPPCSYDAVDHDKLVSLLKSIQTGPFKASGLRK 114

Query: 400  LRSLIEKKDVLQKALIQSGGVQVLNRVILH---EASDFAAFQACEEALGVLSLLPVSDEA 570
            L+ L+EK D LQK LI+SGG++VL  V+ H   E SDF AF+ACEEALGVLSLLP+SD+A
Sbjct: 115  LKVLVEKNDELQKDLIRSGGIEVLGCVMSHVVVENSDFTAFRACEEALGVLSLLPLSDDA 174

Query: 571  TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGL----GVDVFKSLL 738
            T  L+LKPD +K +  +++RGSAEAR HAMS+L+KIS+++ EW   +     VD+ KSLL
Sbjct: 175  TVELILKPDSLKPMMVIIQRGSAEARVHAMSILMKISKISNEWITKMVTDQDVDIVKSLL 234

Query: 739  ELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKAL 918
            +LLSDEISTKLSS+SLDVLLEIV+  +S K R++A+EAGAV +L+ELLP+A+RH CEK L
Sbjct: 235  DLLSDEISTKLSSSSLDVLLEIVA--TSNKNRLKAIEAGAVCILLELLPDASRHNCEKVL 292

Query: 919  LLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEE 1098
            LLLKRLCEC EGR+AF +HGLGVAAVSKKI+RVSE ATKLGV+I+WLMS F P EKL+EE
Sbjct: 293  LLLKRLCECAEGRSAFADHGLGVAAVSKKIMRVSEMATKLGVQIMWLMSSFHPREKLLEE 352

Query: 1099 MVAFGAVRKLLALLHGNER-SSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            M+ FG VRKLLALLH + R SST EKA+ + KLHG  WRQ  CFP E KD +RL
Sbjct: 353  MMVFGTVRKLLALLHIDGRSSSTNEKAIKMMKLHGAVWRQYHCFPSELKDFLRL 406


>ref|XP_008796330.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Phoenix
            dactylifera]
          Length = 410

 Score =  496 bits (1276), Expect = e-170
 Identities = 260/412 (63%), Positives = 316/412 (76%), Gaps = 6/412 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            ME  P LFRCPISMELMEDPVTI+TGVTYERKNIEKWLFTYKKSTCPATMQ L   DLTP
Sbjct: 1    MEEAPPLFRCPISMELMEDPVTISTGVTYERKNIEKWLFTYKKSTCPATMQCLRNLDLTP 60

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            NHTLKRLI+S+      D  A               E   L SLL  +++ PFK S L+K
Sbjct: 61   NHTLKRLITSW----PLDHEARTPTEAPRPCPTNAIEHENLVSLLTSIESTPFKVSSLKK 116

Query: 400  LRSLIEKKDVLQKALIQSGGVQVLNRVILH---EASDFAAFQACEEALGVLSLLPVSDEA 570
            LRS++EK++ +++  ++SGG+Q L R++     +++DF  F+ACEEALGVLSLLP+S++A
Sbjct: 117  LRSIVEKRNEVRENFLKSGGIQALGRIMFQVAADSTDFTTFRACEEALGVLSLLPLSNQA 176

Query: 571  TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGLG---VDVFKSLLE 741
            +  LLLKP+C+K +  +L+RGSAEAR H M++LLKIS+ N  WA  +G   +DVFKSLLE
Sbjct: 177  SVELLLKPECMKPMMVMLQRGSAEARLHTMAILLKISKTNHCWAHTIGDQGMDVFKSLLE 236

Query: 742  LLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALL 921
            LLSDEISTKLSS SLD+LLEIV+A  SKK  ++A+EAGAV +L+ELLP+A +H CEK LL
Sbjct: 237  LLSDEISTKLSSCSLDLLLEIVAA--SKKNPLKAIEAGAVCILIELLPDARQHNCEKMLL 294

Query: 922  LLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEM 1101
            LLKRLCECPEGR+AF +HGLGVA VSKKILRVSE  TKLGVKILWLM   LPTEK++ +M
Sbjct: 295  LLKRLCECPEGRSAFADHGLGVAVVSKKILRVSELGTKLGVKILWLMCTSLPTEKVLHDM 354

Query: 1102 VAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            + +G V KLLALLH   RSSTKEKA+ I +LHGG WRQ  CFPCE KD +RL
Sbjct: 355  MVYGLVEKLLALLHIEGRSSTKEKAMKIIRLHGGAWRQYRCFPCELKDYLRL 406


>ref|XP_010911082.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Elaeis guineensis]
          Length = 411

 Score =  486 bits (1251), Expect = e-166
 Identities = 253/412 (61%), Positives = 315/412 (76%), Gaps = 6/412 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            ME  P  FRCPISMELMEDPVTI+TGVTYERKNIEKWLFTYKKSTCPATMQ L+ FDLTP
Sbjct: 1    MEEAPPFFRCPISMELMEDPVTISTGVTYERKNIEKWLFTYKKSTCPATMQHLKNFDLTP 60

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            NHTLKRLI+S+     D +  +              E  +L SLL  + + PFK S L+ 
Sbjct: 61   NHTLKRLIASW---LLDHEAKSTPPAAPQPCQTNAIEHEKLVSLLSSIDSTPFKVSSLKN 117

Query: 400  LRSLIEKKDVLQKALIQSGGVQVLNRV---ILHEASDFAAFQACEEALGVLSLLPVSDEA 570
            LRS++E K+ +Q+  I+SGG++ L  +   I+ E +DF  F+ACEEAL VLS+LP+S+EA
Sbjct: 118  LRSILENKNEVQEHFIRSGGIEALGHIMFQIVLENTDFTTFRACEEALAVLSILPLSNEA 177

Query: 571  TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGLG---VDVFKSLLE 741
            +  LLLKP+C+K +  +L+RGSA+AR H M++LLKIS+    W   +G   +D+F+SLLE
Sbjct: 178  SVELLLKPECMKPMMIMLQRGSADARLHTMAILLKISKTKHSWTHAIGEQEMDIFRSLLE 237

Query: 742  LLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALL 921
            LLSDEISTKLSS SLDVLLEIV+   SKK   +A+EAGAV +L+ELLP+A++  CEK LL
Sbjct: 238  LLSDEISTKLSSCSLDVLLEIVAV--SKKNGRKAIEAGAVCILIELLPDASQRSCEKILL 295

Query: 922  LLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEM 1101
            LLKRLCE PEGR+AF +HGLGVAAVSKKILRVS+  TKLGVKILWLM  FLPTEK++++M
Sbjct: 296  LLKRLCEYPEGRSAFADHGLGVAAVSKKILRVSDLGTKLGVKILWLMCNFLPTEKVLDDM 355

Query: 1102 VAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            +A+G V+KLL LLH + RSSTK+KA+ I KLHGG WR+ PCFPCE KD +RL
Sbjct: 356  MAYGLVKKLLVLLHIDGRSSTKDKAMKIIKLHGGVWRKYPCFPCELKDYLRL 407


>ref|XP_020096550.1| E3 ubiquitin-protein ligase PUB23-like [Ananas comosus]
 gb|OAY73942.1| E3 ubiquitin-protein ligase PUB23 [Ananas comosus]
          Length = 413

 Score =  457 bits (1175), Expect = e-155
 Identities = 242/418 (57%), Positives = 312/418 (74%), Gaps = 13/418 (3%)
 Frame = +1

Query: 43   EAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPN 222
            E  PLLFRCPISM+LM DPVTIATGV+YERKNIEKWL TYK+STCPATMQ L+T DLTPN
Sbjct: 4    EEAPLLFRCPISMDLMADPVTIATGVSYERKNIEKWLLTYKRSTCPATMQHLDTLDLTPN 63

Query: 223  HTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKL 402
            HTLKRLIS++ D+   +                     +L S L  +++ PFK + LR L
Sbjct: 64   HTLKRLISTWLDKQSANPNTTDQR-----------STGDLISRLTDIESSPFKVNSLRSL 112

Query: 403  RSLIEKKDVLQKALIQSGGVQVLNRVILH-----EASDFAAFQACEEALGVLSLLPVSDE 567
            +S++E  + +Q  L++SGG++VL R++       + +DF+AF+ACEEA GV+S+LP+ DE
Sbjct: 113  KSMLETSEEMQNRLLRSGGIEVLARIMSQATAAADITDFSAFRACEEAAGVVSVLPLYDE 172

Query: 568  ATSG---LLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGLGVD----VF 726
            A+S    +LL+P CVK + A+L+RGSAEAR   M +L+K++R+N++W D LG D    V 
Sbjct: 173  ASSSAAEILLRPKCVKPMTAILQRGSAEARIATMDILVKMARINRKWIDALGDDQNTEVL 232

Query: 727  KSLLELLSDEIST-KLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHG 903
            KSLL+LLSDEIST KLSS SL+VLL IV+   SK  R RAV+ GAV VL+ELLP+A+RH 
Sbjct: 233  KSLLDLLSDEISTRKLSSLSLEVLLSIVAG--SKGGRSRAVDVGAVPVLIELLPDADRHT 290

Query: 904  CEKALLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTE 1083
            CE+ LLLLKRLCECPEGR+AF EH +GVAAV+KKILRVS+  TK+GVKILWL+  F PTE
Sbjct: 291  CERILLLLKRLCECPEGRSAFAEHAMGVAAVAKKILRVSDGGTKVGVKILWLVCSFFPTE 350

Query: 1084 KLVEEMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            K+++EM+A GAV+KLLAL+H + R STKEKA+ I ++HG  WRQ PCFP E +D +RL
Sbjct: 351  KVLDEMMASGAVKKLLALVHVDGRPSTKEKAIKIIRMHGMTWRQYPCFPGELRDYLRL 408


>gb|OVA01288.1| U box domain [Macleaya cordata]
          Length = 420

 Score =  449 bits (1156), Expect = e-152
 Identities = 230/409 (56%), Positives = 302/409 (73%), Gaps = 6/409 (1%)
 Frame = +1

Query: 49   VPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPNHT 228
            +PL FRCPISME+M++PVTI+TGVTYERKNIE+W FTYKK TCPATMQ LE F LTPNHT
Sbjct: 15   IPLYFRCPISMEVMKNPVTISTGVTYERKNIEQWFFTYKKKTCPATMQTLENFYLTPNHT 74

Query: 229  LKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKLRS 408
            LKRLI ++ +R    +                 + +EL SLL  +++ PFK + L+KLRS
Sbjct: 75   LKRLIIAWQERESKSE------DKPSTSPCISIKHDELVSLLTNIESTPFKVNSLKKLRS 128

Query: 409  LIEKKDVLQKALIQSGGVQVLNRVILH---EASDFAAFQACEEALGVLSLLPVSDEATSG 579
             IE  + ++   +Q GG++VL+R+I+    E SDF  F+ACEEALGVL  LP+SDEA+  
Sbjct: 129  CIELGNEVKLDFVQLGGIEVLSRIIIQVLIETSDFVTFRACEEALGVLYQLPISDEASMD 188

Query: 580  LLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEW---ADGLGVDVFKSLLELLS 750
            LL KP+C+KS+A +L+RGSAEAR H +++   +S+V   W       G+D+FKSLLEL+S
Sbjct: 189  LLSKPECMKSMAIMLQRGSAEARFHTVTIFRNMSKVEYNWNWVVQDQGMDLFKSLLELVS 248

Query: 751  DEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALLLLK 930
            DEI TK SS +LDVL+EI+    SKKTR++A+EAGA+ VL+ELLP++N+  CE+ LL++K
Sbjct: 249  DEICTKASSCALDVLIEILG--KSKKTRLKAIEAGAICVLIELLPDSNKSKCERMLLIIK 306

Query: 931  RLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEMVAF 1110
             LCEC EGR AF +H LG+ A++KK+LRVS  ATK GVKILWL+S F PTEK++E+M+ F
Sbjct: 307  LLCECAEGRLAFVDHKLGIVALTKKLLRVSNVATKFGVKILWLISSFHPTEKVIEDMLVF 366

Query: 1111 GAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            G+V+KLL LL  + RSSTK+KAV I KLHG  W+Q PCFP E KD +RL
Sbjct: 367  GSVKKLLGLLQIDGRSSTKDKAVKIIKLHGNSWKQFPCFPSELKDYLRL 415


>ref|XP_010275264.2| PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Nelumbo nucifera]
          Length = 416

 Score =  445 bits (1145), Expect = e-150
 Identities = 234/415 (56%), Positives = 310/415 (74%), Gaps = 7/415 (1%)
 Frame = +1

Query: 34   QAMEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDL 213
            Q+ME +PL FRCPISME+M+DPVTI+TGVTYERKNIE W F Y K TCPATMQ +++FDL
Sbjct: 9    QSME-IPLYFRCPISMEIMKDPVTISTGVTYERKNIETWFFKYNKKTCPATMQCIQSFDL 67

Query: 214  TPNHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCL 393
            TPN TLK LI ++ DR      ++              +  EL SLL  +++ PFK S L
Sbjct: 68   TPNLTLKSLILAWQDRESQFRLSSSPIST---------KHTELVSLLTTMESTPFKVSSL 118

Query: 394  RKLRSLIE-KKDVLQKALIQSGGVQVLNRVILH---EASDFAAFQACEEALGVLSLLPVS 561
            +KLR++IE   D +    +QSGGV VL+R+I+    ++SDF AF+ACEEALGVL  LP+S
Sbjct: 119  KKLRAIIEFNDDAVMGDFVQSGGVDVLSRIIVQILVDSSDFMAFRACEEALGVLHQLPLS 178

Query: 562  DEATSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWA---DGLGVDVFKS 732
            DE++  LL KP+C+KS+A +L+RGSAEAR H +S+  K+++V   W+   +  G+D+FKS
Sbjct: 179  DESSIQLLSKPECMKSMAIMLQRGSAEARLHTVSMFRKMAKVEYNWSWVVEDQGMDLFKS 238

Query: 733  LLELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEK 912
            LLELLSDE+STK SS++LD+L+EI++A  SKK R++A+EAGAV VL+ELLP+ANR  CEK
Sbjct: 239  LLELLSDELSTKASSSALDILIEILNA--SKKNRLKAIEAGAVCVLIELLPDANRSKCEK 296

Query: 913  ALLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLV 1092
             L+++K LCEC EGR A  EH LG+AA++KKILRVS+  TK GVKI WL+  F PTEK+V
Sbjct: 297  MLVIIKLLCECAEGRLALVEHSLGIAAITKKILRVSDLVTKYGVKIFWLICNFHPTEKVV 356

Query: 1093 EEMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            EEM+ +G+V+KL+ALLH + RSSTK+KA+ I KLH   WRQ PCFP E K+ ++L
Sbjct: 357  EEMLVYGSVKKLVALLHMDGRSSTKDKALKIIKLHENSWRQYPCFPSELKEYLKL 411


>gb|PIA32753.1| hypothetical protein AQUCO_04400152v1 [Aquilegia coerulea]
          Length = 417

 Score =  429 bits (1104), Expect = e-144
 Identities = 214/408 (52%), Positives = 305/408 (74%), Gaps = 6/408 (1%)
 Frame = +1

Query: 52   PLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPNHTL 231
            PL FRCPISME+M++PVT++TGVTYE+KNIEKWL++YKK TCPATMQ LE F+LTPNHTL
Sbjct: 14   PLYFRCPISMEVMKEPVTVSTGVTYEKKNIEKWLYSYKKKTCPATMQALENFNLTPNHTL 73

Query: 232  KRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKLRSL 411
            KRLI ++  +      A+              + +EL +LL  + + PF  + L+KLR++
Sbjct: 74   KRLILTWQAKESKSVVASPLSTSV--------KHDELVALLATIGSTPFMVTSLKKLRAI 125

Query: 412  IEKKDVLQKALIQSGGVQVLNRVILH---EASDFAAFQACEEALGVLSLLPVSDEATSGL 582
            +E  D ++   +QSGG++VL+R+I+    + SDF AF++CEEALG+L  +P++ EA+  L
Sbjct: 126  VEMDDEIKLDFVQSGGIEVLSRIIVQGLVDFSDFIAFRSCEEALGILYQVPLASEASIEL 185

Query: 583  LLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGL---GVDVFKSLLELLSD 753
            L +P+C+KS+A +L+RGSAEAR H +++   +++    W+  +   G+D+F SLLEL+SD
Sbjct: 186  LSQPECMKSMAVMLQRGSAEARLHTITIFRNMAKTEYNWSGVVHDQGMDLFSSLLELVSD 245

Query: 754  EISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALLLLKR 933
            EI+ K SS +LDVL+EI+  R+SKK R++A+E+GA+ +L+ELLP++N+  CE+ LL++K 
Sbjct: 246  EINNKASSCALDVLIEIL--RASKKNRLKAIESGAMCILIELLPDSNKSKCERMLLIIKL 303

Query: 934  LCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEMVAFG 1113
            LCEC EGR A  +H LG+ A+SKKILRVSE  T+LG+KILWL+S F PTEK++EEM+ FG
Sbjct: 304  LCECAEGRLALVDHSLGIVAISKKILRVSEVTTRLGIKILWLISSFHPTEKVLEEMLMFG 363

Query: 1114 AVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            +VRKLL LLH + RSSTK+KA+ I KLHG +WR+ PCFP E +D + L
Sbjct: 364  SVRKLLGLLHIDGRSSTKDKAMKIIKLHGDKWRKYPCFPNELRDYLGL 411


>ref|XP_018728284.1| PREDICTED: E3 ubiquitin-protein ligase PUB23 [Eucalyptus grandis]
 gb|KCW76777.1| hypothetical protein EUGRSUZ_D01133 [Eucalyptus grandis]
          Length = 407

 Score =  426 bits (1096), Expect = e-143
 Identities = 225/405 (55%), Positives = 295/405 (72%), Gaps = 7/405 (1%)
 Frame = +1

Query: 52   PLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPNHTL 231
            PL FRCPISME+MEDPVTIATG+TYERKNIEKW F YKK TCPATMQ +E+F +TPNHTL
Sbjct: 4    PLDFRCPISMEVMEDPVTIATGMTYERKNIEKWFFAYKKKTCPATMQSVESFGITPNHTL 63

Query: 232  KRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKLRSL 411
            KRLI ++  +                        +E+ SLL+ +++ PFK + L+KLRS+
Sbjct: 64   KRLIITWQSK-------GGPGTPSSESRKSTERHDEIVSLLEALESSPFKVTSLKKLRSM 116

Query: 412  IEKKDVLQKALIQSGGVQVLNRV---ILHEASDFAAFQACEEALGVLSLLPVSDEA-TSG 579
            IE  D  +   +QSGG+ ++ R+   +L + SDF+ F+ACEEALGVL  L +S EA T  
Sbjct: 117  IETDDEAKADFVQSGGIGIIVRIASQVLEDGSDFSIFRACEEALGVLHQLSLSTEAKTLE 176

Query: 580  LLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGL---GVDVFKSLLELLS 750
             L KP+ VK + A+L+RGSAEAR HA+ +L  +++    W   L   G+D+FKSLLEL+S
Sbjct: 177  TLSKPEAVKCVTAMLQRGSAEARSHAIIILRNMAQSETHWTFILQDHGIDLFKSLLELVS 236

Query: 751  DEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALLLLK 930
            DEI T+ SS +L+VL+E++SA  SKK+RVRA+EAGAV VLVELLPE+NR   EK L+LLK
Sbjct: 237  DEICTRASSYALEVLVEMLSA--SKKSRVRAIEAGAVCVLVELLPESNRSKSEKILMLLK 294

Query: 931  RLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEMVAF 1110
             LCEC EGR A  +HG+G+AAV+KK+L VS AATKL VKILWL+  F P+E+++EEM+ +
Sbjct: 295  LLCECAEGRLAMVDHGMGIAAVTKKMLNVSNAATKLSVKILWLIGSFHPSERVLEEMLIY 354

Query: 1111 GAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKD 1245
            G+V+KLLALLH + RSSTKEK + +FK+H   W++CPCFP E KD
Sbjct: 355  GSVKKLLALLHIDGRSSTKEKVIKMFKMHCNSWQRCPCFPNEMKD 399


>ref|XP_010269983.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Nelumbo nucifera]
          Length = 418

 Score =  424 bits (1090), Expect = e-142
 Identities = 226/415 (54%), Positives = 297/415 (71%), Gaps = 7/415 (1%)
 Frame = +1

Query: 34   QAMEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDL 213
            ++ME  PL FRCPISME+M+DPV I+TGVTYERK+IEKW F Y K TCPATMQ +  FDL
Sbjct: 9    ESMET-PLHFRCPISMEIMKDPVIISTGVTYERKSIEKWFFKYNKKTCPATMQTIHNFDL 67

Query: 214  TPNHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCL 393
            TPN TLK LI S++ +       +              + +EL SLL  +++ PF  + L
Sbjct: 68   TPNLTLKSLILSWHHQQSHHSQPSSPPAVSI-------KHHELVSLLTTIESTPFMVTSL 120

Query: 394  RKLRSLIEKKDVLQKALIQSGGVQVLNRVILH----EASDFAAFQACEEALGVLSLLPVS 561
            +KLR++I   D ++   +QSGGV+VL R+I+     + SDF AF+ACEEAL VL  L  S
Sbjct: 121  KKLRAVIGFNDEVKDDFVQSGGVEVLTRIIVQILLVDTSDFTAFRACEEALAVLHQLSFS 180

Query: 562  DEATSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWA---DGLGVDVFKS 732
            DEA+  LL KP+C+ S+A +L+RGSAEAR H +S+   +++V   W    +  G+D+FKS
Sbjct: 181  DEASIELLSKPECINSMAIMLQRGSAEARLHTVSIFRTMAKVEYNWTWVVEDQGIDLFKS 240

Query: 733  LLELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEK 912
            LLELLSDEI+TK SS++LDVL+EI++A  SKK R++A+EAGAV VL+ELLP+ANR  CEK
Sbjct: 241  LLELLSDEINTKASSSALDVLIEILNA--SKKNRLKAIEAGAVCVLIELLPDANRSKCEK 298

Query: 913  ALLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLV 1092
             L+L+K LCEC EGR A  EH LG+AAV+KKILRVS+ ATK  VKI WL+    PT  ++
Sbjct: 299  ILVLIKLLCECAEGRLALAEHRLGIAAVTKKILRVSDTATKYSVKIFWLICTSHPTNNVL 358

Query: 1093 EEMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            +EM+  G+V+KLLALLH + RSSTK+KA+ + KLHG  WR+ PCFPCE KD +RL
Sbjct: 359  KEMLVSGSVKKLLALLHMDGRSSTKDKALKMIKLHGNSWRRYPCFPCELKDYLRL 413


>ref|XP_018845148.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Juglans regia]
 ref|XP_018856063.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Juglans regia]
 ref|XP_018807245.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Juglans regia]
          Length = 415

 Score =  418 bits (1075), Expect = e-140
 Identities = 225/414 (54%), Positives = 301/414 (72%), Gaps = 8/414 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            M   P  F CPIS+ELM+DPVTI+TGV+YERKNIEKW F YKK+TCPATMQ ++ FD+TP
Sbjct: 1    MNVPPYDFLCPISIELMKDPVTISTGVSYERKNIEKWFFAYKKTTCPATMQSIKNFDITP 60

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            NHTLKRLI ++ ++      ++              + +++ SLL  +++ PFK S L+K
Sbjct: 61   NHTLKRLIIAWLNKAISQSCSSSSSSSSTSRPSV--KHDDILSLLNTIESSPFKVSSLKK 118

Query: 400  LRSLIEKKDVLQKA-LIQSGGVQVLNRV---ILHEASDFAAFQACEEALGVLSLLPVSDE 567
            L+S+IE  D   KA  IQS GV+VL ++   IL E+SDF  F+ACEEALGVL  LP S+E
Sbjct: 119  LQSIIEIGDDEIKADFIQSNGVEVLVQIVVQILSESSDFGTFRACEEALGVLQKLPFSEE 178

Query: 568  A-TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGL---GVDVFKSL 735
              T  LL KP+ +KS+  +L+RGSAEAR + +++  K+++ N +W   +   G+D FKSL
Sbjct: 179  EKTLELLSKPESMKSMEIMLQRGSAEARLYTLTIFQKMAKSNYDWNSFIQDRGIDFFKSL 238

Query: 736  LELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKA 915
            LEL+SDEI TK SS +L+VL+EI+    SKK+R++A+EAGAV VL+ELLP+++R  CEKA
Sbjct: 239  LELVSDEICTKASSCALEVLIEILGG--SKKSRLQAIEAGAVCVLIELLPDSSRSKCEKA 296

Query: 916  LLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVE 1095
            LLLLK LCEC EGR A  EHG+G+AA+SKK+L +S  ATK+ VKILWL+  F PT++L+E
Sbjct: 297  LLLLKLLCECAEGRQALVEHGMGIAAISKKMLHLSSTATKIVVKILWLICSFHPTDRLLE 356

Query: 1096 EMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            EMV  G+V+KLLALLH + RSSTK+K + IFKLHG  WR+ PCFP E K+ + L
Sbjct: 357  EMVVCGSVKKLLALLHIDGRSSTKDKTIKIFKLHGNSWRRYPCFPSELKEYLAL 410


>gb|ONI22128.1| hypothetical protein PRUPE_2G109000 [Prunus persica]
          Length = 446

 Score =  419 bits (1076), Expect = e-139
 Identities = 216/410 (52%), Positives = 296/410 (72%), Gaps = 7/410 (1%)
 Frame = +1

Query: 37   AMEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLT 216
            AM+  P  FRCPISMELM++PVTI+TGVTYERKNIEKW FTYKK TCPATMQ +E F + 
Sbjct: 31   AMDFSPCHFRCPISMELMKEPVTISTGVTYERKNIEKWFFTYKKKTCPATMQSIENFGII 90

Query: 217  PNHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLR 396
            PNHTLKRLI S+    D     +              + +E+++LL  + + PFK + ++
Sbjct: 91   PNHTLKRLIVSWQSNEDSKSSCSSPSSSQSQPCHSA-KHDEIQALLSTIVSSPFKVNSIK 149

Query: 397  KLRSLIEKKDVLQKALIQSGGVQVLNRVI---LHEASDFAAFQACEEALGVLSLLPVSDE 567
            KLRS+IE  D ++   IQS GV+VL +++   + E+SDF  F+ACEEAL VL  LP+S+E
Sbjct: 150  KLRSIIEIDDEMKDDFIQSNGVEVLVQILDQTIMESSDFVTFRACEEALCVLQQLPISEE 209

Query: 568  A-TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWA---DGLGVDVFKSL 735
              T  LL K +  +S+A +L+RGSAEAR H +++  K+++   +W+      G+D FKSL
Sbjct: 210  GKTFELLSKQESTRSMAIMLQRGSAEARLHTVTIFRKMAKTEYDWSFIVQDQGIDFFKSL 269

Query: 736  LELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKA 915
            LEL+SDEI +K SS +L+VL+EI+  R+SKK R+RA+EAGAV VL+ELL ++NR  CE+ 
Sbjct: 270  LELVSDEICSKASSCALEVLIEIL--RASKKNRLRAIEAGAVCVLIELLQDSNRSKCERM 327

Query: 916  LLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVE 1095
            L L+K LCEC EGR A  EHG+G+ A++KK+L VS AATK+GVKI+WL+  F PTE+++E
Sbjct: 328  LHLIKLLCECAEGRQALVEHGMGIVAITKKMLHVSNAATKIGVKIIWLVCNFHPTERVLE 387

Query: 1096 EMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKD 1245
            EM+ +G+V+KLLALLH + RSSTK+K + IFK+HG  W++ PCFP + KD
Sbjct: 388  EMLMYGSVKKLLALLHMDGRSSTKDKVLKIFKMHGNSWKRYPCFPYDLKD 437


>ref|XP_007219082.2| E3 ubiquitin-protein ligase PUB23 [Prunus persica]
          Length = 415

 Score =  417 bits (1072), Expect = e-139
 Identities = 215/409 (52%), Positives = 295/409 (72%), Gaps = 7/409 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            M+  P  FRCPISMELM++PVTI+TGVTYERKNIEKW FTYKK TCPATMQ +E F + P
Sbjct: 1    MDFSPCHFRCPISMELMKEPVTISTGVTYERKNIEKWFFTYKKKTCPATMQSIENFGIIP 60

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            NHTLKRLI S+    D     +              + +E+++LL  + + PFK + ++K
Sbjct: 61   NHTLKRLIVSWQSNEDSKSSCSSPSSSQSQPCHSA-KHDEIQALLSTIVSSPFKVNSIKK 119

Query: 400  LRSLIEKKDVLQKALIQSGGVQVLNRVI---LHEASDFAAFQACEEALGVLSLLPVSDEA 570
            LRS+IE  D ++   IQS GV+VL +++   + E+SDF  F+ACEEAL VL  LP+S+E 
Sbjct: 120  LRSIIEIDDEMKDDFIQSNGVEVLVQILDQTIMESSDFVTFRACEEALCVLQQLPISEEG 179

Query: 571  -TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWA---DGLGVDVFKSLL 738
             T  LL K +  +S+A +L+RGSAEAR H +++  K+++   +W+      G+D FKSLL
Sbjct: 180  KTFELLSKQESTRSMAIMLQRGSAEARLHTVTIFRKMAKTEYDWSFIVQDQGIDFFKSLL 239

Query: 739  ELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKAL 918
            EL+SDEI +K SS +L+VL+EI+  R+SKK R+RA+EAGAV VL+ELL ++NR  CE+ L
Sbjct: 240  ELVSDEICSKASSCALEVLIEIL--RASKKNRLRAIEAGAVCVLIELLQDSNRSKCERML 297

Query: 919  LLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEE 1098
             L+K LCEC EGR A  EHG+G+ A++KK+L VS AATK+GVKI+WL+  F PTE+++EE
Sbjct: 298  HLIKLLCECAEGRQALVEHGMGIVAITKKMLHVSNAATKIGVKIIWLVCNFHPTERVLEE 357

Query: 1099 MVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKD 1245
            M+ +G+V+KLLALLH + RSSTK+K + IFK+HG  W++ PCFP + KD
Sbjct: 358  MLMYGSVKKLLALLHMDGRSSTKDKVLKIFKMHGNSWKRYPCFPYDLKD 406


>ref|XP_008232231.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Prunus mume]
          Length = 415

 Score =  417 bits (1071), Expect = e-139
 Identities = 216/409 (52%), Positives = 296/409 (72%), Gaps = 7/409 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            M+ +P  FRCPISMELM++PVTI+TGVTYERKNIEKW FTYKK TCPATMQ +E F + P
Sbjct: 1    MDFLPCHFRCPISMELMKEPVTISTGVTYERKNIEKWFFTYKKKTCPATMQSIENFGIIP 60

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            NHTLKRLI S+    D     +              + +E+++LL  V + PFK + ++K
Sbjct: 61   NHTLKRLIVSWQSNEDSKSSCSSPSSSQSQPCPSA-KHDEIQALLSTVVSSPFKVNSIKK 119

Query: 400  LRSLIEKKDVLQKALIQSGGVQVLNRVI---LHEASDFAAFQACEEALGVLSLLPVSDEA 570
            LRS+IE  D ++   IQS GV+VL +++   + ++SDF  F+ACEEAL VL  LP+S+E 
Sbjct: 120  LRSIIEIDDEMKDDFIQSNGVEVLVQILDQTIMDSSDFVTFRACEEALCVLHQLPISEEG 179

Query: 571  -TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWA---DGLGVDVFKSLL 738
             T  LL K +  +S+A +L+RGSAEAR H +++  K+ +   +W+      G+D FKSLL
Sbjct: 180  KTFELLSKQESTRSMAIMLQRGSAEARLHTITIFRKMVKTEYDWSFIVQDQGIDFFKSLL 239

Query: 739  ELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKAL 918
            EL+SDEI +K SS +L+VL+EI+  R+SKK R+RA+EAGAV VL+ELL ++NR  CEK L
Sbjct: 240  ELVSDEICSKASSCALEVLIEIL--RASKKNRLRAIEAGAVCVLIELLQDSNRSKCEKML 297

Query: 919  LLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEE 1098
             L+K LCEC EGR A  EHG+G+AA+++K+L VS AATK+GVKI+WL+  F PTE+++EE
Sbjct: 298  HLIKLLCECAEGRQALVEHGMGIAAITQKMLHVSNAATKIGVKIIWLVCNFHPTERVLEE 357

Query: 1099 MVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKD 1245
            M+ +G+V+KLLALLH + RSSTK+K V IFK+HG  W++ PCFP + K+
Sbjct: 358  MLMYGSVKKLLALLHMDGRSSTKDKVVKIFKMHGNSWKRYPCFPYDLKE 406


>ref|XP_004308738.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Fragaria vesca
            subsp. vesca]
          Length = 400

 Score =  414 bits (1064), Expect = e-138
 Identities = 214/408 (52%), Positives = 289/408 (70%), Gaps = 8/408 (1%)
 Frame = +1

Query: 37   AMEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLT 216
            AM+  P  FRCPISMELM+DPVTI+TGVTYER NIEKW FTYKK TCPATMQR+E FD  
Sbjct: 2    AMDFPPYHFRCPISMELMKDPVTISTGVTYERVNIEKWFFTYKKKTCPATMQRIENFDTI 61

Query: 217  PNHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLR 396
            PNHTLKRLI ++ ++ D                    +  E+E+LL  + + PFK S L+
Sbjct: 62   PNHTLKRLILAWQNQGDSKS--------SELSNSPSDKHEEIEALLSTIMSSPFKVSSLK 113

Query: 397  KLRSLIEKKDVLQKALIQSGGVQVLNRVI---LHEASDFAAFQACEEALGVLSLLPVSDE 567
            KLRS+I+  +  +   I   GV+VL +++   L E SDF  F+ACEEAL +L  LP+S+E
Sbjct: 114  KLRSIIDMGEETKNDFISCKGVEVLVQILDQTLTENSDFVTFRACEEALCILHQLPISEE 173

Query: 568  ATSG--LLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEW---ADGLGVDVFKS 732
                  LL K D  +S+A +L+RGSAEAR H +++  K+++ N +W       G+D FKS
Sbjct: 174  EGDSFKLLSKQDSTRSMAIMLQRGSAEARLHTITIFKKMAKTNYDWNFVIQDQGLDFFKS 233

Query: 733  LLELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEK 912
            LLEL+SDEI +K SS +L+VL+EI+  R+SKK +++A+EAGAV VL+ELLPE++R  CEK
Sbjct: 234  LLELVSDEICSKASSCALEVLIEIL--RASKKNKLKAIEAGAVCVLIELLPESSRSRCEK 291

Query: 913  ALLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLV 1092
             L ++K LCEC EGR A  EHG+G+  +SKK++ VS  ATK+GVKI+WL+  F PTEK++
Sbjct: 292  MLQVIKLLCECAEGRLALVEHGMGIVVISKKMMHVSNVATKIGVKIMWLVCNFHPTEKVL 351

Query: 1093 EEMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCE 1236
            EEM+ +G+V+KLLALLH + RSSTK+K V IF+LHG  W++ PC+PC+
Sbjct: 352  EEMLIYGSVKKLLALLHMDGRSSTKDKVVKIFRLHGNSWKRYPCYPCD 399


>ref|XP_024169270.1| E3 ubiquitin-protein ligase PUB23-like [Rosa chinensis]
          Length = 416

 Score =  410 bits (1053), Expect = e-136
 Identities = 213/410 (51%), Positives = 291/410 (70%), Gaps = 8/410 (1%)
 Frame = +1

Query: 37   AMEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLT 216
            AM+  P  FRCPISM+LM+DPVTI+TGVTYER NIEKW FTYKK TCPATMQR+  FD  
Sbjct: 2    AMDFPPYHFRCPISMDLMKDPVTISTGVTYERVNIEKWFFTYKKKTCPATMQRISNFDTI 61

Query: 217  PNHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLR 396
            PNHTLKRLI ++ ++ D    ++              +  E+++LL  + + PFK S L+
Sbjct: 62   PNHTLKRLILAWQNQGDSKSSSSSSCELLNSHSD---KHEEIKALLSTIMSSPFKVSSLK 118

Query: 397  KLRSLIEKKDVLQKALIQSGGVQVLNRV---ILHEASDFAAFQACEEALGVLSLLPVSDE 567
            KLRS+I+  +  +   I S GV+VL ++   IL E SDF  F+ACEEAL +L  LP+S+E
Sbjct: 119  KLRSIIDMGEETRNDFISSNGVEVLVQILDQILTENSDFITFRACEEALCILHQLPISEE 178

Query: 568  A--TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEW---ADGLGVDVFKS 732
               T  LL + D  +S+  +L+RGSAEAR H +++  K+++ N +W       G+D FKS
Sbjct: 179  EGNTFKLLSRQDSTRSMTIMLQRGSAEARLHTITIFKKMAKTNYDWNFVIQDQGIDFFKS 238

Query: 733  LLELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEK 912
            LLEL+SDEI +K SS +L+VL+EI+  R+SKK ++RA+EAGAV VL+ELL E++R  CEK
Sbjct: 239  LLELVSDEICSKASSCALEVLIEIL--RASKKNKLRAIEAGAVCVLIELLQESSRSRCEK 296

Query: 913  ALLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLV 1092
             L ++K LCEC EGR A  EHG+G+ A+SKK++ VS  ATK+GVKI+WL+  F PTEK++
Sbjct: 297  MLQVIKFLCECAEGRQALVEHGMGIVAISKKMMHVSNVATKIGVKIMWLVCNFHPTEKVL 356

Query: 1093 EEMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWK 1242
            EEM+ +G+V+KLLALLH + RSSTK+K V IFK+HG  W++ PC+P + K
Sbjct: 357  EEMLIYGSVKKLLALLHMDGRSSTKDKVVKIFKMHGNSWKRYPCYPSDLK 406


>ref|XP_022743887.1| E3 ubiquitin-protein ligase PUB23-like [Durio zibethinus]
          Length = 408

 Score =  409 bits (1052), Expect = e-136
 Identities = 214/403 (53%), Positives = 293/403 (72%), Gaps = 9/403 (2%)
 Frame = +1

Query: 61   FRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPNHTLKRL 240
            FRCPISME+M+DPVTI TGV+YERKN+EKW   Y K+TCPATMQ L++ D+TPNHTLKRL
Sbjct: 10   FRCPISMEVMKDPVTILTGVSYERKNVEKWFHKYNKTTCPATMQPLQSLDMTPNHTLKRL 69

Query: 241  ISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKLRSLIEK 420
            I ++ D+      +               +  E+ SL   +++ PFK + LRKLRS+++ 
Sbjct: 70   ILAWQDKLVTPSSS---------PSHPSIKHEEMVSLFSTLESSPFKVTSLRKLRSIVQL 120

Query: 421  KDVLQKALIQSGGVQVLNRVILHEA----SDFAAFQACEEALGVLSLLPV--SDEATSGL 582
             D  +   I+SGGV+ + RV++++     SDF +FQACEEALG+L LLP+   ++ T  L
Sbjct: 121  GDDTRSDFIRSGGVEAVVRVLVNQTVVDNSDFVSFQACEEALGLLHLLPLISEEDKTFQL 180

Query: 583  LLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEW---ADGLGVDVFKSLLELLSD 753
            L KP+ +KS+A VL+RGSAEAR +A+++  +I++ + +W       G+D+FKSLLEL+ D
Sbjct: 181  LSKPEPMKSMAIVLQRGSAEARLYAITIFRQIAKTDFDWNFLLQDQGIDMFKSLLELVYD 240

Query: 754  EISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALLLLKR 933
            E+ +K SS +L+VL+EI++  SSKK+R++A+EAGAV V VELLP++N+  CEK LLL+K 
Sbjct: 241  ELCSKASSCALEVLIEILA--SSKKSRLKAIEAGAVCVFVELLPDSNKSKCEKMLLLIKL 298

Query: 934  LCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEMVAFG 1113
            LCEC EGR A  EHGLG+ AVSKK+L VS +ATKLGVKILWL S F PTE+++EEM+ +G
Sbjct: 299  LCECAEGRMALVEHGLGIPAVSKKLLHVSNSATKLGVKILWLTSTFHPTERVLEEMLIYG 358

Query: 1114 AVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWK 1242
            AV+KL+AL+H   RSSTKEK + I KLHG  WR+ PCFP + K
Sbjct: 359  AVKKLVALIHLEGRSSTKEKVLNILKLHGNSWRRHPCFPSDLK 401


>gb|PKA53966.1| E3 ubiquitin-protein ligase PUB23 [Apostasia shenzhenica]
          Length = 409

 Score =  409 bits (1051), Expect = e-136
 Identities = 221/407 (54%), Positives = 288/407 (70%), Gaps = 8/407 (1%)
 Frame = +1

Query: 40   MEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTP 219
            ME +P +FRCPIS++LME+PVTIATGVTYER++IEKWLFTYKK+TCPATMQ L  FDL P
Sbjct: 1    MEELPSMFRCPISLQLMENPVTIATGVTYERRSIEKWLFTYKKTTCPATMQPLHNFDLIP 60

Query: 220  NHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRK 399
            N TLK LI S+  R+                      R +L S+L  +++ PFK + L++
Sbjct: 61   NRTLKSLIDSW--RSQSRRPTPPPSLPSSSSSSSPTVRAQLTSILSAMESSPFKVTYLKR 118

Query: 400  LRSLIEKKDVLQKALIQSGGVQVLNRVILH-----EASDFAAFQACEEALGVLSLLPVS- 561
            L+S I+         I SGGV  L R++       + SDF+ F+ACEEAL VL  LP++ 
Sbjct: 119  LKSFIDGGRENPAVFIGSGGVDSLGRILSQANSAADGSDFSTFRACEEALAVLDRLPLAA 178

Query: 562  DEATSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGL--GVDVFKSL 735
            DEA+  LL KP+ +K +  VL+RG AEAR HAM++L +IS  N   AD     +DVFKSL
Sbjct: 179  DEASIALLSKPESIKPMLDVLQRGGAEARLHAMAILQRISAANPAVADDFHHDIDVFKSL 238

Query: 736  LELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKA 915
            L+LLSDE S  L+S+SLD+LL IV   +SK  R++A+EAGAV VLVELLP+A R   EKA
Sbjct: 239  LDLLSDETSKCLTSSSLDLLLMIVE--NSKMNRLKAIEAGAVRVLVELLPDAGRRRGEKA 296

Query: 916  LLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVE 1095
            LLLLKRLCEC EGR+A  +HG+G+AAV+ +++R S+  TK+GV+ILWL+  FLPT+K+V+
Sbjct: 297  LLLLKRLCECAEGRSAIADHGMGIAAVASRVVRASDVETKMGVRILWLVGSFLPTKKVVD 356

Query: 1096 EMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCE 1236
            EM+A GAVRKLL LLHG+  S+TKEKA+ + ++HG  WRQ PCFPCE
Sbjct: 357  EMMACGAVRKLLGLLHGDGSSATKEKALKMIRMHGDAWRQYPCFPCE 403


>ref|XP_008346772.1| PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Malus domestica]
          Length = 421

 Score =  409 bits (1052), Expect = e-136
 Identities = 215/409 (52%), Positives = 292/409 (71%), Gaps = 7/409 (1%)
 Frame = +1

Query: 52   PLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPNHTL 231
            P  FRCPISMELM+DPVTI+TGVTYER NIEKW F+YKK TCPATMQ LE FD+ PNHTL
Sbjct: 19   PYHFRCPISMELMKDPVTISTGVTYERNNIEKWFFSYKKKTCPATMQSLENFDMIPNHTL 78

Query: 232  KRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKLRSL 411
            KRLI ++  +T++D  A               + +E+++LL  + + PFK + ++KLRS+
Sbjct: 79   KRLIVAW--QTEED--AKNVICSTSSTPRPSAKHDEIQALLNTIMSSPFKVNSIKKLRSI 134

Query: 412  IEKKDVLQKALIQSGGVQVLNRV---ILHEASDFAAFQACEEALGVLSLLPVSDEA-TSG 579
            I+  D ++   I+S GV+VL ++   IL E+SDF  F+ACEEAL +L  LP+S E  T  
Sbjct: 135  IDLDDEMKNDFIRSNGVEVLVQILDQILLESSDFTTFRACEEALCILHQLPISGEGKTFK 194

Query: 580  LLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWA---DGLGVDVFKSLLELLS 750
            LL K +  +S+  +L+RGS EAR H +++  K+++   +W+      G+D FKSLLELLS
Sbjct: 195  LLSKQESTRSMTVMLQRGSTEARLHTITIFKKMAKTEYDWSFVIQDQGIDFFKSLLELLS 254

Query: 751  DEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKALLLLK 930
            DEI +K SS +L VL+EI++A  SKK ++RA+EAGAV VL+ELLP++NR  CEK L ++K
Sbjct: 255  DEICSKASSCALKVLIEILTA--SKKNKLRAIEAGAVCVLIELLPDSNRSKCEKMLQVIK 312

Query: 931  RLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEEMVAF 1110
             LCEC EGR A  EH +G+ AVSK +L VS AATK+GVKI+WL+  F PTE+++EEM+  
Sbjct: 313  LLCECSEGRQALVEHSMGIVAVSKNMLHVSNAATKIGVKIIWLICNFHPTERVLEEMLIC 372

Query: 1111 GAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKDLVRL 1257
            G+V+KLLALL  + RSSTK+K V IFK+HG  W++ PCFP + KD + L
Sbjct: 373  GSVKKLLALLQMDSRSSTKDKVVKIFKMHGNSWKRYPCFPWDLKDYLDL 421


>gb|PRQ56801.1| putative aminoacyltransferase, E1 ubiquitin-activating enzyme [Rosa
            chinensis]
          Length = 438

 Score =  410 bits (1053), Expect = e-136
 Identities = 213/410 (51%), Positives = 291/410 (70%), Gaps = 8/410 (1%)
 Frame = +1

Query: 37   AMEAVPLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLT 216
            AM+  P  FRCPISM+LM+DPVTI+TGVTYER NIEKW FTYKK TCPATMQR+  FD  
Sbjct: 2    AMDFPPYHFRCPISMDLMKDPVTISTGVTYERVNIEKWFFTYKKKTCPATMQRISNFDTI 61

Query: 217  PNHTLKRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLR 396
            PNHTLKRLI ++ ++ D    ++              +  E+++LL  + + PFK S L+
Sbjct: 62   PNHTLKRLILAWQNQGDSKSSSSSSCELLNSHSD---KHEEIKALLSTIMSSPFKVSSLK 118

Query: 397  KLRSLIEKKDVLQKALIQSGGVQVLNRV---ILHEASDFAAFQACEEALGVLSLLPVSDE 567
            KLRS+I+  +  +   I S GV+VL ++   IL E SDF  F+ACEEAL +L  LP+S+E
Sbjct: 119  KLRSIIDMGEETRNDFISSNGVEVLVQILDQILTENSDFITFRACEEALCILHQLPISEE 178

Query: 568  A--TSGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEW---ADGLGVDVFKS 732
               T  LL + D  +S+  +L+RGSAEAR H +++  K+++ N +W       G+D FKS
Sbjct: 179  EGNTFKLLSRQDSTRSMTIMLQRGSAEARLHTITIFKKMAKTNYDWNFVIQDQGIDFFKS 238

Query: 733  LLELLSDEISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEK 912
            LLEL+SDEI +K SS +L+VL+EI+  R+SKK ++RA+EAGAV VL+ELL E++R  CEK
Sbjct: 239  LLELVSDEICSKASSCALEVLIEIL--RASKKNKLRAIEAGAVCVLIELLQESSRSRCEK 296

Query: 913  ALLLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLV 1092
             L ++K LCEC EGR A  EHG+G+ A+SKK++ VS  ATK+GVKI+WL+  F PTEK++
Sbjct: 297  MLQVIKFLCECAEGRQALVEHGMGIVAISKKMMHVSNVATKIGVKIMWLVCNFHPTEKVL 356

Query: 1093 EEMVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWK 1242
            EEM+ +G+V+KLLALLH + RSSTK+K V IFK+HG  W++ PC+P + K
Sbjct: 357  EEMLIYGSVKKLLALLHMDGRSSTKDKVVKIFKMHGNSWKRYPCYPSDLK 406


>ref|XP_002264927.3| PREDICTED: E3 ubiquitin-protein ligase PUB23 [Vitis vinifera]
          Length = 422

 Score =  408 bits (1049), Expect = e-136
 Identities = 218/409 (53%), Positives = 293/409 (71%), Gaps = 11/409 (2%)
 Frame = +1

Query: 52   PLLFRCPISMELMEDPVTIATGVTYERKNIEKWLFTYKKSTCPATMQRLETFDLTPNHTL 231
            P+ FRCPISMELM+DPVTI+TGVTYERKNIEKWLF+Y K TCP TMQR+ +FD+TPNHTL
Sbjct: 15   PVDFRCPISMELMQDPVTISTGVTYERKNIEKWLFSYNKKTCPTTMQRINSFDITPNHTL 74

Query: 232  KRLISSFNDRTDDDDFANXXXXXXXXXXXXXXERNELESLLQKVQTGPFKASCLRKLRSL 411
            KRLI ++ +                       + +EL SLL  +++ PFK + L+KLR++
Sbjct: 75   KRLILAWQNEE--------AARSCESRPVRSLKHDELVSLLATMESTPFKVNSLKKLRAI 126

Query: 412  IEKKDVLQKALIQSGGVQVLNRVI---LHEASDFAAFQACEEALGVLSL---LPVSDEAT 573
            +     ++   ++ GGV+VL ++I   L E  DF AF ACEEALGVL     L   DE T
Sbjct: 127  VGLGHEVKDDFMRCGGVEVLVQIIAQILIETFDFVAFTACEEALGVLHQYLSLSEEDEKT 186

Query: 574  SGLLLKPDCVKSIAAVLRRGSAEARGHAMSVLLKISRVNQEWADGL----GVDVFKSLLE 741
              LL KP+ +KS+A +L+RGS EAR +A+++  K+++V+  W   +    G + F+SLLE
Sbjct: 187  IQLLSKPESIKSLAIMLQRGSGEARFYAVTIFQKLAQVDYNWKKFVIQDQGFEFFRSLLE 246

Query: 742  LLSDE-ISTKLSSASLDVLLEIVSARSSKKTRVRAVEAGAVSVLVELLPEANRHGCEKAL 918
            L+SD+ I TK S+++L++L+EI+   SSK +R++ +EAGAV +L+ELLP++NR  CEK L
Sbjct: 247  LVSDDQICTKASASALELLIEILG--SSKTSRLKVIEAGAVCILIELLPDSNRSKCEKML 304

Query: 919  LLLKRLCECPEGRAAFDEHGLGVAAVSKKILRVSEAATKLGVKILWLMSKFLPTEKLVEE 1098
            LLLK LCEC EGR A  EHGLG+AA+SKK+L VS AATK+ VKILWL+  + P+E+++EE
Sbjct: 305  LLLKLLCECAEGRLALVEHGLGIAAISKKLLHVSSAATKITVKILWLICSYHPSERVLEE 364

Query: 1099 MVAFGAVRKLLALLHGNERSSTKEKAVGIFKLHGGRWRQCPCFPCEWKD 1245
            ++ FG+V+KLLALLH + RSSTKEK V IFKLHG  WRQ PCFPCE KD
Sbjct: 365  LLIFGSVKKLLALLHIDNRSSTKEKVVKIFKLHGNSWRQNPCFPCELKD 413