BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cheilocostus21_contig00019611
         (2936 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_018683242.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1139   0.0  
ref|XP_018683243.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...  1033   0.0  
ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoe...   978   0.0  
ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elae...   954   0.0  
ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]...   943   0.0  
gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]           928   0.0  
ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelu...   925   0.0  
ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus offic...   908   0.0  
ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium cate...   897   0.0  
gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]    874   0.0  
ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber] ...   864   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   859   0.0  
ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]        858   0.0  
ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]...   850   0.0  
gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olito...   848   0.0  
ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prun...   843   0.0  
gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]                 841   0.0  
ref|XP_024168065.1| isoamylase 2, chloroplastic [Rosa chinensis]...   841   0.0  
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             841   0.0  
ref|XP_017978673.1| PREDICTED: isoamylase 2, chloroplastic [Theo...   841   0.0  

>ref|XP_018683242.1| PREDICTED: isoamylase 2, chloroplastic-like [Musa acuminata subsp.
            malaccensis]
          Length = 862

 Score = 1139 bits (2947), Expect = 0.0
 Identities = 555/759 (73%), Positives = 634/759 (83%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEFLSSFRNEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIWDSSG 180
            VVVG+ G N+SVHIE LS  ++E++LIYE+S  +   SLVA ++    +++MPF+ +SSG
Sbjct: 101  VVVGAVGVNFSVHIEVLSVSQDELVLIYEMSRSDPPGSLVADIEKGIGAVIMPFVRNSSG 160

Query: 181  RYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPLGVSI 360
            RYTLDL+FDS KAPFYLSF+LHL SD+GV+  EI+THRK  FC+PVGLGPGYPAPLG S+
Sbjct: 161  RYTLDLDFDSAKAPFYLSFMLHLCSDAGVVASEIKTHRKTRFCVPVGLGPGYPAPLGASV 220

Query: 361  SDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSMESIAGYVNY 540
            SDDG VNF+LFSR +E V+LCLY+GKTE PSLEI+LDPYVNRTG+IWHVSMESI  YV+Y
Sbjct: 221  SDDGMVNFSLFSRKAENVVLCLYEGKTEVPSLEIELDPYVNRTGDIWHVSMESIEDYVSY 280

Query: 541  GYRCKGMVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTFEWGNDA 720
            GYRCKG VEK  G+D+Q VL DPYAKMV+NLL   G++   +K LGSLE EP F+W  D 
Sbjct: 281  GYRCKGPVEKRGGFDMQHVLLDPYAKMVRNLLSVQGDTMTPTKCLGSLEMEPIFDWSGDV 340

Query: 721  HPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVLLEPVFL 900
            HP LP E+LVVYRLNVGQFT D S GLPED+AGTF GVSEKV HFKELGVN++LLEP+F 
Sbjct: 341  HPQLPTEKLVVYRLNVGQFTIDNSSGLPEDVAGTFGGVSEKVEHFKELGVNAILLEPIFP 400

Query: 901  FNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFTHTGEET 1080
            F+V KGPYFPYHFFSV D YGQ+ + ASA  SMKEMIK LH EGIEVLMEVVFTH+ E  
Sbjct: 401  FDVNKGPYFPYHFFSVMDEYGQEHNAASAINSMKEMIKTLHSEGIEVLMEVVFTHSAEGG 460

Query: 1081 DTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDFHIDGFC 1260
            D  S V+S RGIDSSSYY ++  +G+  NSLLKC NPIVQ LIIDSLR WVV+FH+DGFC
Sbjct: 461  DADSHVISFRGIDSSSYYIVDKNVGSGTNSLLKCNNPIVQQLIIDSLRHWVVEFHVDGFC 520

Query: 1261 FINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFPHWKIWA 1440
            FINSSSMARG+ G HLSRPPLVEAIAFDPVLS+ KI+ADCWSP+DMSY EIQFPHWK WA
Sbjct: 521  FINSSSMARGQNGDHLSRPPLVEAIAFDPVLSRTKIVADCWSPLDMSYMEIQFPHWKAWA 580

Query: 1441 QMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFASRGPAFSFNFVAKNFGLPLVDLVSF 1620
            +MN RFC DVRNFLRG+GLLS+ ATRLCGSGDLF+SRGPAFSFNFV KN GLPLVDLVSF
Sbjct: 581  EMNMRFCSDVRNFLRGEGLLSNLATRLCGSGDLFSSRGPAFSFNFVTKNVGLPLVDLVSF 640

Query: 1621 SDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDELGYSTGG 1800
            S  ELASELSWNCGDEGPTN  TVLETRLKQIRNFLF+LFVSLGVPVLNMGDE GYSTGG
Sbjct: 641  STAELASELSWNCGDEGPTNNNTVLETRLKQIRNFLFVLFVSLGVPVLNMGDECGYSTGG 700

Query: 1801 SRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIVWYASNQS 1980
            S L +DR+PI+W SL   FS QI KFIA+L S R RR DIFQS+HFLKVENIVW+ SNQS
Sbjct: 701  SPLYDDRKPINWDSLGTGFSKQITKFIAYLGSLRIRRGDIFQSKHFLKVENIVWFGSNQS 760

Query: 1981 HPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQLEGNSWLRLV 2160
             P+WDDPTCKFLA+ LKSE +   S+S  GDLF+CFNAS+  ETV+LP Q EGN WLRLV
Sbjct: 761  EPKWDDPTCKFLALALKSEKNFDMSNSNCGDLFICFNASNNLETVVLPEQPEGNVWLRLV 820

Query: 2161 DTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEA 2277
            DTSLAFP  F ++ DP+V KA+G SSYEL+PHSCALFEA
Sbjct: 821  DTSLAFPGIFSNSCDPNVQKAEGSSSYELKPHSCALFEA 859


>ref|XP_018683243.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic [Musa
            acuminata subsp. malaccensis]
          Length = 705

 Score = 1033 bits (2670), Expect = 0.0
 Identities = 506/706 (71%), Positives = 577/706 (81%)
 Frame = +1

Query: 154  MPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPG 333
            MPF+ + SGRYTLDL+FDS KAPFYLSF+LHL SD+GV+  EI+THRK  FC+PVG GPG
Sbjct: 1    MPFVCNYSGRYTLDLDFDSAKAPFYLSFMLHLCSDAGVVASEIKTHRKTRFCVPVGFGPG 60

Query: 334  YPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSM 513
            YPA LG S+SDDG VNF+LF R++E V LCLY+GKTE PSLEI+LDPYVNRTG+IWHVSM
Sbjct: 61   YPASLGASVSDDGMVNFSLFLRNAESVFLCLYEGKTEVPSLEIELDPYVNRTGDIWHVSM 120

Query: 514  ESIAGYVNYGYRCKGMVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKE 693
            ESI  YV+YGYRCKG V+K    ++Q VL DPYAKMV+NLL    ++   +K+LGSLE E
Sbjct: 121  ESIVDYVSYGYRCKGPVDKEVDXNMQHVLLDPYAKMVRNLLSVQSDTMTPTKYLGSLEIE 180

Query: 694  PTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVN 873
            P F+W  D HP LP E+LVVYRLNVGQFT+D S GLPED+AGTF GVSEKV HFKELGVN
Sbjct: 181  PIFDWSGDVHPRLPTEKLVVYRLNVGQFTRDNSIGLPEDVAGTFGGVSEKVEHFKELGVN 240

Query: 874  SVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEV 1053
            ++LLEP+F F+V KGPYFPYHFFS+ D YG++ + ASA  S+K MIK LH E IEVLMEV
Sbjct: 241  AILLEPIFPFDVNKGPYFPYHFFSLMDEYGREHNAASAINSLKGMIKTLHSECIEVLMEV 300

Query: 1054 VFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWV 1233
            VFT   E  D  S V+S RGIDSSSY  ++  +G+  NSLLKC NPIVQ LIIDSLR WV
Sbjct: 301  VFTQV-EGGDADSHVISFRGIDSSSYCIVDKNVGSGTNSLLKCNNPIVQQLIIDSLRHWV 359

Query: 1234 VDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEI 1413
            V+FH+DGFCFINSSSMARG+ G HLSRPPLVEAIAFDPVLS+ KI+ADCWSP+DMSY EI
Sbjct: 360  VEFHVDGFCFINSSSMARGQNGDHLSRPPLVEAIAFDPVLSRTKIVADCWSPLDMSYMEI 419

Query: 1414 QFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFASRGPAFSFNFVAKNFG 1593
            QFPHWK WA+MN RFC D +NFLRG+GLLSD ATRLCGSGDLF+S GPAFSFNFVAKNFG
Sbjct: 420  QFPHWKAWAEMNMRFCSDAKNFLRGEGLLSDLATRLCGSGDLFSSAGPAFSFNFVAKNFG 479

Query: 1594 LPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMG 1773
            LPLVDLV FS  ELASELSWNCGDEGPTN  TVLETRLKQIRNFLF+LFVSLGVPVLNMG
Sbjct: 480  LPLVDLVKFSTAELASELSWNCGDEGPTNNNTVLETRLKQIRNFLFVLFVSLGVPVLNMG 539

Query: 1774 DELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVEN 1953
            DE GYSTGGS L +DR+PI+W SL   FS QI KFIA+L S R  R DIFQS+HFLKVEN
Sbjct: 540  DECGYSTGGSPLYDDRKPINWDSLGTGFSKQITKFIAYLGSLRIPRGDIFQSKHFLKVEN 599

Query: 1954 IVWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQL 2133
            IV + SNQS P+WDDPTCKFLA+ LKSE +    +S  GDLF+CFNAS+  ETV+LP   
Sbjct: 600  IVLFGSNQSEPKWDDPTCKFLALALKSEKNFDMLNSNGGDLFICFNASNNLETVVLPEPT 659

Query: 2134 EGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALF 2271
            EGN WLRLVDTSLA P FF ++ DP+  KA+G SSYEL+P S  LF
Sbjct: 660  EGNVWLRLVDTSLALPGFFSNSYDPNGQKAEGSSSYELKPQSGVLF 705


>ref|XP_008799133.1| PREDICTED: isoamylase 2, chloroplastic [Phoenix dactylifera]
          Length = 853

 Score =  978 bits (2527), Expect = 0.0
 Identities = 487/767 (63%), Positives = 584/767 (76%), Gaps = 4/767 (0%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEFLSSFR--NEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIWDS 174
            V+VG++   Y+V +E  S  R   E  L+     +    S +    ++ +     F+ +S
Sbjct: 95   VLVGTKSMRYAVRVEVSSLPRAVGEDGLVMNWGMFRSDSSQLLAQDSQVA-----FVRNS 149

Query: 175  SGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPLGV 354
             G Y ++LEFDS + PFYLSFLL  SS   V   EIRTHRK  FC+PVGLGPG+P PLGV
Sbjct: 150  LGGYMVELEFDSARIPFYLSFLL--SSPPSV--SEIRTHRKTKFCVPVGLGPGWPMPLGV 205

Query: 355  SISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSMESIAGYV 534
            SISDDG VNF+LFSR++E V+LCL+DGKT EPSLE+ LDPYVNRTG+IWHVSMES+  +V
Sbjct: 206  SISDDGAVNFSLFSRNAEGVVLCLFDGKTREPSLEMDLDPYVNRTGDIWHVSMESMGNFV 265

Query: 535  NYGYRCKG--MVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTFEW 708
            +YGYRCKG  + +K   +    VL DPYAK++ + +PD GES  ++K LGSL  E +F+W
Sbjct: 266  SYGYRCKGSGLWDKEDRFHAHHVLLDPYAKVLGDFVPDRGESVSLAKCLGSLGFEHSFDW 325

Query: 709  GNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVLLE 888
              D HP LP+E+LVVYRLNVGQFTK+K+ GLPE+++GTF G+ EKV HF+ LGVN+VLLE
Sbjct: 326  SGDVHPRLPMEKLVVYRLNVGQFTKNKTSGLPENVSGTFAGLIEKVEHFRTLGVNAVLLE 385

Query: 889  PVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFTHT 1068
            P+F F+  KGPYFPYHFFS    YG      SA  SMKEM+K LH  GIEVL+EV FT T
Sbjct: 386  PIFSFDEHKGPYFPYHFFSPMHSYGHASDGVSAINSMKEMVKMLHAHGIEVLLEVAFTQT 445

Query: 1069 GEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDFHI 1248
            GEE D   Q +S RGID+SSYY ++G +G+ A ++LKC NP+VQ LI+DSLR WVV+FH+
Sbjct: 446  GEEGDAACQTISFRGIDNSSYYIVDGDLGSGAYNVLKCSNPVVQQLILDSLRHWVVEFHV 505

Query: 1249 DGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFPHW 1428
            DGFCFINSS +ARG  G +LSRPPLVEAIA DP+LSK KIIADCWSP+D SY EIQFPHW
Sbjct: 506  DGFCFINSSFLARGLNGDYLSRPPLVEAIAVDPILSKTKIIADCWSPLDNSYMEIQFPHW 565

Query: 1429 KIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFASRGPAFSFNFVAKNFGLPLVD 1608
            K WA+MN RFC DVRNFLRG+GLL D ATRLCGSGDLF+SRGPAFSFN++ KNFGL LVD
Sbjct: 566  KRWAEMNTRFCSDVRNFLRGEGLLRDLATRLCGSGDLFSSRGPAFSFNYITKNFGLTLVD 625

Query: 1609 LVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDELGY 1788
            LVSFS+ +LASELSWNCG+EG TN  TVL+ RLKQIRNFLF+LFVSLGVPVLNMGDE GY
Sbjct: 626  LVSFSNGDLASELSWNCGEEGSTNNNTVLDIRLKQIRNFLFVLFVSLGVPVLNMGDECGY 685

Query: 1789 STGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIVWYA 1968
            STGGS     RQPI W  LQ  F  QI +FIAF+SS R RR DIFQ R+FL++ENI W+ 
Sbjct: 686  STGGSPSYIGRQPIGWNGLQTVFGRQITQFIAFMSSLRMRRGDIFQRRNFLELENIDWHG 745

Query: 1969 SNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQLEGNSW 2148
            S+QS P W  P+CKFLAMTL++E+D K S+S  GDLF+ FNAS  PE+ +LP Q EG  W
Sbjct: 746  SSQSRPNWGKPSCKFLAMTLRAEMDDKLSNSDDGDLFISFNASAHPESTVLPEQSEGVVW 805

Query: 2149 LRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERIT 2289
            LRLVDTSL FP FF   SDP VH+A GLSSYEL+PHSC LFEA+R T
Sbjct: 806  LRLVDTSLPFPGFFSSDSDPSVHQAAGLSSYELKPHSCVLFEAKRST 852


>ref|XP_010938750.1| PREDICTED: isoamylase 2, chloroplastic [Elaeis guineensis]
          Length = 851

 Score =  954 bits (2465), Expect = 0.0
 Identities = 464/712 (65%), Positives = 556/712 (78%), Gaps = 2/712 (0%)
 Frame = +1

Query: 160  FIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYP 339
            F+ +S G Y ++LEFDS + PFYLSFLL   S S     EIRTH+K  FC+PVG+GPG P
Sbjct: 144  FVQNSLGGYMVELEFDSVEIPFYLSFLLSSPSVS-----EIRTHKKTKFCVPVGIGPGCP 198

Query: 340  APLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSMES 519
             PLGVSISDDG +NF++FSR++E V+L L+DG+T EPSLEI LDPYVNRTG+IWHVSMES
Sbjct: 199  MPLGVSISDDGAINFSVFSRNAEGVVLYLFDGRTREPSLEIDLDPYVNRTGDIWHVSMES 258

Query: 520  IAGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKE 693
            I  Y  YGYRCKG+   +KG  +    +L DPYAK++ + + D GES  ++K LGSL+ E
Sbjct: 259  IENYAGYGYRCKGLALWDKGDKFHAHDILLDPYAKVLGDFVLDQGESMSLAKCLGSLDIE 318

Query: 694  PTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVN 873
              F+W  D HP LP+E+LVVYRLNVGQFTK K+  LPE++AGTF G+ EKV H K LGVN
Sbjct: 319  HGFDWTGDVHPCLPMEKLVVYRLNVGQFTKHKTSRLPENVAGTFAGLIEKVEHLKSLGVN 378

Query: 874  SVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEV 1053
            +VLLEP+F F+ +KGPYFPYHFFS  + YG      SA  SMK+M+K LH  GIEVL+EV
Sbjct: 379  AVLLEPIFSFDEQKGPYFPYHFFSPMNSYGYASDGVSAIDSMKKMVKMLHAHGIEVLLEV 438

Query: 1054 VFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWV 1233
             FT TGE  D   + +S RGID+SSYY ++  +G  A++ LKC NP+VQ LI+DSLR WV
Sbjct: 439  AFTQTGEGEDAACRTISFRGIDNSSYYIVDRDLGYGAHNALKCSNPVVQQLILDSLRHWV 498

Query: 1234 VDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEI 1413
            ++FH+DGFCFINSS +A+G  G +L RPPLVEAIAFDP+LSK KIIADCWSP D SY EI
Sbjct: 499  IEFHVDGFCFINSSFLAQGLNGDYLYRPPLVEAIAFDPILSKTKIIADCWSPPDNSYMEI 558

Query: 1414 QFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFASRGPAFSFNFVAKNFG 1593
            +FPHWK WA+MN RFC DVRNFLRG+GLLS+ ATRLCGSGDLF+SRGPAFSFN++ KNFG
Sbjct: 559  RFPHWKRWAEMNTRFCSDVRNFLRGEGLLSNLATRLCGSGDLFSSRGPAFSFNYITKNFG 618

Query: 1594 LPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMG 1773
            L LVDLVS+SD +LASELSWNCG+EGPTN  TVL+ RLKQIRNFLF+LFVSLGVPVLNMG
Sbjct: 619  LSLVDLVSYSDGDLASELSWNCGEEGPTNNNTVLDLRLKQIRNFLFVLFVSLGVPVLNMG 678

Query: 1774 DELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVEN 1953
            DE GYSTGG+    DR+PI W  L+  F  QI +FIAFLSS R RR DIFQ R FLKVEN
Sbjct: 679  DECGYSTGGTPSYIDRKPIGWNGLKTVFGRQITQFIAFLSSLRVRRGDIFQRRDFLKVEN 738

Query: 1954 IVWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQL 2133
            I W+ S+QS P W +P+CKFLA+TLK+E+D K S+S  GDLF+ FNASD  E+ +LP Q 
Sbjct: 739  IDWHGSSQSQPNWREPSCKFLAVTLKAEMDDKLSNSNDGDLFISFNASDLLESAVLPEQS 798

Query: 2134 EGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERIT 2289
            EG  WLRLVDT+L FP FF   SDPDVH+A GLSSY+L+PHSC LFEA+R T
Sbjct: 799  EGIVWLRLVDTALPFPGFFSSDSDPDVHQAAGLSSYDLKPHSCVLFEAKRST 850


>ref|XP_020087102.1| isoamylase 2, chloroplastic [Ananas comosus]
 ref|XP_020087103.1| isoamylase 2, chloroplastic [Ananas comosus]
          Length = 863

 Score =  943 bits (2438), Expect = 0.0
 Identities = 462/769 (60%), Positives = 574/769 (74%), Gaps = 8/769 (1%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIE--FLSSFRNEVMLIYEISAYEFSHSLVAGVKNECS----SIVMPF 162
            VVV S+ G Y V  E  FL  F  E  L+   + +    SL+    ++ S    +  +PF
Sbjct: 97   VVVRSEYGKYVVRFEVTFLPQFGTEEGLVLNWTMFTSDSSLLLVPCSQASEGTVTAKVPF 156

Query: 163  IWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPA 342
            + +SSG +  +L FD+ K PFYLSFLL+ +        EIR+HRK +FC+PVGLG G P 
Sbjct: 157  VQNSSGSFLAELVFDASKVPFYLSFLLNSAGS------EIRSHRKTNFCVPVGLGSGQPM 210

Query: 343  PLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSMESI 522
            PLGVS+SDDG  NF+LFS+++E VILCLYDG  +EP+LEI+LDPY+N TG+IWHVS+E++
Sbjct: 211  PLGVSVSDDGLTNFSLFSKNAEGVILCLYDGIKDEPALEIELDPYINCTGDIWHVSLENV 270

Query: 523  AGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEP 696
              YV+YGYRCKG +   KG  + ++ VL DPYAK++ N  PD G    +S+ L SL KE 
Sbjct: 271  EEYVSYGYRCKGPILWRKGDRFHMKHVLLDPYAKVIGNFFPDHGGKVSLSRCLASLAKES 330

Query: 697  TFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNS 876
             F+W  D  P LP+E+LVVYRLNVG  TKDKS GL E+ AGTF G+ EK+ +FK LGVN+
Sbjct: 331  AFDWSGDTSPCLPMEKLVVYRLNVGLCTKDKSSGLSENAAGTFSGLIEKIEYFKTLGVNA 390

Query: 877  VLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVV 1056
            VLLEP+F F+ +KGPYFP+HFFS    YG    +AS T SMKEMIK +H  G+EVL+EVV
Sbjct: 391  VLLEPIFPFDEKKGPYFPFHFFSPMMSYGHGRDSASGTNSMKEMIKSMHARGMEVLLEVV 450

Query: 1057 FTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVV 1236
            F+HT E  D  SQ++S RGID+SSYY ++G + + AN+ L C NPIVQ LI+DSL  WVV
Sbjct: 451  FSHTSEGGDAASQMISFRGIDNSSYYIVDGDVRSGANNALNCNNPIVQRLILDSLHHWVV 510

Query: 1237 DFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQ 1416
            ++H+DGFCF+NSSS+ R   G +LSRPPL+EAIAFDP+LSK KIIADCWSPIDMS  EIQ
Sbjct: 511  EYHVDGFCFVNSSSLVRSSNGNNLSRPPLLEAIAFDPILSKTKIIADCWSPIDMSSMEIQ 570

Query: 1417 FPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFASRGPAFSFNFVAKNFGL 1596
            FPHWK WA+MN+RF  DVRNFLRG+ LLSD ATR+CGSGDLF+SRGPA+SFN++ KNFGL
Sbjct: 571  FPHWKKWAEMNSRFSVDVRNFLRGEALLSDLATRICGSGDLFSSRGPAYSFNYITKNFGL 630

Query: 1597 PLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGD 1776
            PLVDLVSFS  +LASELSWNCG+EGPT   +VL+TRLKQIRNFLFILFVSLGVPVLNMGD
Sbjct: 631  PLVDLVSFSSIDLASELSWNCGEEGPTGNSSVLQTRLKQIRNFLFILFVSLGVPVLNMGD 690

Query: 1777 ELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENI 1956
            E GYS GGS    DR+P DW SL+  F  QI +F+A+LSS R+RR DIFQ + FLKVENI
Sbjct: 691  ECGYSNGGSPSYNDRRPFDWNSLRTGFGLQITQFVAYLSSLRNRRGDIFQRKDFLKVENI 750

Query: 1957 VWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQLE 2136
             W  SNQ+ P W D +CKFL++  K+++D + S    GDLF+ FNASD PE  +LP Q E
Sbjct: 751  CWNGSNQTEPNWGDSSCKFLSVMFKADVDGRASKLHRGDLFIGFNASDHPEVALLPEQSE 810

Query: 2137 GNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAER 2283
            G  WLRLVDT+L FP FF   SDP+VH+  GLS+YE++ HSC+LFEA+R
Sbjct: 811  GTVWLRLVDTALPFPGFFASYSDPNVHQVAGLSAYEIKSHSCSLFEAKR 859


>gb|OAY83378.1| Isoamylase 2, chloroplastic [Ananas comosus]
          Length = 1205

 Score =  928 bits (2398), Expect = 0.0
 Identities = 456/765 (59%), Positives = 565/765 (73%), Gaps = 8/765 (1%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIE--FLSSFRNEVMLIYEISAYEFSHSLVAGVKNECS----SIVMPF 162
            VVV S+ G Y V  E  FL  F  E  L+   + +    SL+    ++ S    +  +PF
Sbjct: 97   VVVRSEYGKYVVRFEVTFLPQFGTEEGLVLNWTMFTSDSSLLLVPCSQASEGTVTAKVPF 156

Query: 163  IWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPA 342
            + +SSG +  +L FD+ K PFYLSFLL+ +        EIR+HRK +FC+PVGLG G P 
Sbjct: 157  VQNSSGSFLAELVFDASKVPFYLSFLLNSAGS------EIRSHRKTNFCVPVGLGSGQPM 210

Query: 343  PLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSMESI 522
            PLGVS+SDDG  NF+LFS+++E VILCLYDG  +EP+LEI+LDPY+N TG+IWHVS+E++
Sbjct: 211  PLGVSVSDDGLTNFSLFSKNAEGVILCLYDGIKDEPALEIELDPYINCTGDIWHVSLENV 270

Query: 523  AGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEP 696
              YV+YGYRCKG +   KG  + ++ VL DPYAK++ N  PD G    +S+ L SL KE 
Sbjct: 271  EEYVSYGYRCKGPILWRKGDRFHMKHVLLDPYAKVIGNFFPDHGGKVSLSRCLASLAKES 330

Query: 697  TFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNS 876
             F+W  D  P LP+E+LVVYRLNVG  TKDKS GL E+ AGTF G+ EK+ +FK LGVN+
Sbjct: 331  AFDWSGDTSPCLPMEKLVVYRLNVGLCTKDKSSGLSENAAGTFSGLIEKIEYFKTLGVNA 390

Query: 877  VLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVV 1056
            VLLEP+F F+ +KGPYFP+HFFS    YG    +AS T SMKEMIK +H  G+EVL+EVV
Sbjct: 391  VLLEPIFPFDEKKGPYFPFHFFSPMMSYGHGRDSASGTNSMKEMIKSMHARGMEVLLEVV 450

Query: 1057 FTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVV 1236
            F+HT E  D  SQ++S RGID+SSYY ++G + + AN+ L C NPIVQ LI+DSL  WVV
Sbjct: 451  FSHTSEGGDAASQMISFRGIDNSSYYIVDGDVRSGANNALNCNNPIVQRLILDSLHHWVV 510

Query: 1237 DFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQ 1416
            ++H+DGFCF+NSSS+ R   G +LSRPPL+EAIAFDP+LSK KIIADCWSPIDMS  EIQ
Sbjct: 511  EYHVDGFCFVNSSSLVRSSNGNNLSRPPLLEAIAFDPILSKTKIIADCWSPIDMSSMEIQ 570

Query: 1417 FPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFASRGPAFSFNFVAKNFGL 1596
            FPHWK WA+MN+RF  DVRNFLRG+ LLSD ATR+CGSGDLF+SRGPA+SFN++ KNFGL
Sbjct: 571  FPHWKKWAEMNSRFSVDVRNFLRGEALLSDLATRICGSGDLFSSRGPAYSFNYITKNFGL 630

Query: 1597 PLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGD 1776
            PLVDLVSFS  +LASELSWNCG+EGPT   +VL+TRLKQIRNFLFILFVSLGVPVLNMGD
Sbjct: 631  PLVDLVSFSSIDLASELSWNCGEEGPTGNSSVLQTRLKQIRNFLFILFVSLGVPVLNMGD 690

Query: 1777 ELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENI 1956
            E GYS GGS    DR+P DW SL+  F  QI +F+A+LSS R+RR DIFQ + FLKVENI
Sbjct: 691  ECGYSNGGSPSYNDRRPFDWNSLRTGFGLQITQFVAYLSSLRNRRGDIFQRKDFLKVENI 750

Query: 1957 VWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQLE 2136
             W  SNQ+ P W D +CKFL++  K++ D + S    GDLF+ FNASD PE  +LP Q E
Sbjct: 751  CWNGSNQTEPNWGDSSCKFLSVMFKADADGRASKLHRGDLFIGFNASDHPEVALLPEQSE 810

Query: 2137 GNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALF 2271
            G  WLRLVDT+L FP FF   SDP+VH+  GLS+YE++ H    F
Sbjct: 811  GTVWLRLVDTALPFPGFFASYSDPNVHQVAGLSAYEIKSHRINTF 855


>ref|XP_010278620.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
 ref|XP_010278628.1| PREDICTED: isoamylase 2, chloroplastic [Nelumbo nucifera]
          Length = 890

 Score =  925 bits (2390), Expect = 0.0
 Identities = 459/778 (58%), Positives = 568/778 (73%), Gaps = 16/778 (2%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEFLSS---------FRNEVMLIYEISAYEFSHSLVAGVKNECSSIV 153
            + VG +  NY+V IE  S          F N  +   + S      S V+  +   S++ 
Sbjct: 111  ITVGRKNTNYTVCIEVSSLPQCNTEDKLFLNWGIFRSDSSCLTLPGSQVSAPETHSSTME 170

Query: 154  MPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPG 333
             P +  SSGR+ L LEF+S +APFYLSF+L  SS++     EIR+HRK +FC+PVG+  G
Sbjct: 171  TPLMQKSSGRHFLQLEFESNQAPFYLSFMLLFSSNTATNNSEIRSHRKTNFCVPVGISSG 230

Query: 334  YPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSM 513
            +PAPLG+S SDDG+VNF+LFSR++E V+LCLYD K+++PSLEI LDPY+NRTGNIWHVSM
Sbjct: 231  HPAPLGISFSDDGSVNFSLFSRNAESVVLCLYDEKSDQPSLEIDLDPYINRTGNIWHVSM 290

Query: 514  ESIAGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLE 687
            ES+A YV+YGYRCKG +  +KG  +D + VL DPYAK++ + LP+  E+    K LG L 
Sbjct: 291  ESVAPYVSYGYRCKGDIHLKKGDRFDARNVLLDPYAKILSSSLPNHSETHSPPKRLGHLC 350

Query: 688  KEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELG 867
            K PTF+W  D  P L +EELVVYRLNV +FT+DKSC LP D+ GTF G+ EK+ HFK LG
Sbjct: 351  KIPTFDWSGDIRPCLEIEELVVYRLNVRRFTEDKSCQLPADVLGTFSGLIEKLHHFKSLG 410

Query: 868  VNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLM 1047
            VN+VLLEPVF F  + GPYFPYHFFS SDLYG      S   SMKEMIKRLH  GIEVL+
Sbjct: 411  VNAVLLEPVFSFGEQNGPYFPYHFFSASDLYGPSYDNVSTINSMKEMIKRLHANGIEVLL 470

Query: 1048 EVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRR 1227
            EVVFTH  E  D+  Q +S RGID+SSYY + G       + L C NPIVQ +I+D L+ 
Sbjct: 471  EVVFTHAAEGGDSSFQTISFRGIDNSSYYILNGDTQLGTRNALNCNNPIVQRMILDILQY 530

Query: 1228 WVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYK 1407
            WV +FHIDGFCF+N+SS+ RG  G +LSRPPLVEAIAFDP+LSK KIIADCW P +M  +
Sbjct: 531  WVTEFHIDGFCFMNASSLLRGLNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPCEMVTR 590

Query: 1408 EIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFA-SRGPAFSFNFVAK 1584
            +I+FPHWK WA++NNRFC D+R FLRG+GLLSD ATRLCGSGD+F   RGPAFSFNF+A+
Sbjct: 591  DIRFPHWKRWAEVNNRFCHDIRKFLRGEGLLSDLATRLCGSGDIFLDGRGPAFSFNFIAR 650

Query: 1585 NFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVL 1764
            NFGLPLVDLVSFS  E++SELSWNCG+EG TN   VLE RLKQIRNFLFIL++S GVP+L
Sbjct: 651  NFGLPLVDLVSFSGNEVSSELSWNCGEEGATNNTVVLERRLKQIRNFLFILYISFGVPIL 710

Query: 1765 NMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLK 1944
            NMGDE G S+ GS    DR+P DW +L+  F+ Q  +FIAFLSS R+RRSD+ Q R+FLK
Sbjct: 711  NMGDECGQSSSGSTSYGDRKPFDWTALRTGFAIQTTEFIAFLSSLRTRRSDLLQKRNFLK 770

Query: 1945 VENIVWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRS----GDLFVCFNASDCPET 2112
            VENI WY SNQS P+W+DP+ KFLA+ LKS++D+  S S S    GDLF+ FNA    E 
Sbjct: 771  VENIEWYGSNQSQPRWEDPSSKFLALRLKSDIDNSQSDSDSSQIRGDLFIAFNAGGHSEG 830

Query: 2113 VILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERI 2286
            VILP   EG  WLRLVDT+L FP FF +  DP + + +GL +YE++ HSCALFEA  +
Sbjct: 831  VILPSPSEGMVWLRLVDTALPFPGFFSNDGDPVLEQMQGLIAYEMKAHSCALFEARSL 888


>ref|XP_020273972.1| isoamylase 2, chloroplastic [Asparagus officinalis]
 ref|XP_020273973.1| isoamylase 2, chloroplastic [Asparagus officinalis]
 gb|ONK62840.1| uncharacterized protein A4U43_C07F8670 [Asparagus officinalis]
          Length = 855

 Score =  908 bits (2346), Expect = 0.0
 Identities = 451/766 (58%), Positives = 557/766 (72%), Gaps = 5/766 (0%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF--LSSFRNEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIWDS 174
            V+VG     Y+V I+   L +  N+++L + +   + +     G    C+    P + + 
Sbjct: 97   VLVGFNNMKYAVRIDVSSLPTRTNDLILSWGVHRSDSNQLDAPG----CTKT--PLVQNL 150

Query: 175  SGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPLGV 354
             GR+++ LEF S + PFYLSFLLH SSD       IRTHR  +FCLPVG+  G+P PLGV
Sbjct: 151  KGRFSVTLEFSSTQIPFYLSFLLHSSSDHPRCESGIRTHRMTNFCLPVGMSAGHPMPLGV 210

Query: 355  SISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSMESIAGYV 534
            S  + G VNFALFSR++E V+L L+D    EP LE++LDPYVNRTG+IWH+SME++  Y 
Sbjct: 211  SRCEGGLVNFALFSRNAESVVLRLFDNNVNEPCLELELDPYVNRTGDIWHLSMENVGSYT 270

Query: 535  NYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTFEW 708
            +YGYRCKG    +KGS +  +QVL DPYAK++ N+ P+ GES  + K LGSL +EP F+W
Sbjct: 271  SYGYRCKGETGSDKGSRFHARQVLLDPYAKLIANVSPEKGESRSLVKCLGSLAEEPIFDW 330

Query: 709  GNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVLLE 888
              D HP LP+E+LVVYRLNVG FTKD+S  LPE++AGTF GV EK+ H K LG+N++LLE
Sbjct: 331  SGDVHPQLPMEKLVVYRLNVGNFTKDESSELPENVAGTFAGVIEKLPHLKSLGINAILLE 390

Query: 889  PVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFTHT 1068
            P+F F+ ++GPYFPYHFFS  + YG    + S   SMK+M+K LH  GIEVL+EVVFTHT
Sbjct: 391  PIFTFDEKEGPYFPYHFFSPMNSYGYKSDSISTINSMKDMVKTLHAHGIEVLLEVVFTHT 450

Query: 1069 GEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDFHI 1248
            GE  D+ SQ  S RGID SSYY +EG +     + +   +P+VQ LI++SL  W+ +FHI
Sbjct: 451  GEGGDSSSQTKSFRGIDYSSYYIVEGDMTQGIYNAVNSNDPVVQQLILESLHYWLTEFHI 510

Query: 1249 DGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFPHW 1428
            DGFCFINSS + RG  G +LS PPLVEAI+FDP+LSK+KIIADCWSP+D SY +I FPHW
Sbjct: 511  DGFCFINSSYLLRGSNGEYLSHPPLVEAISFDPLLSKSKIIADCWSPVDGSYLDIHFPHW 570

Query: 1429 KIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFA-SRGPAFSFNFVAKNFGLPLV 1605
            K WA+MN RFC DVRNFLRG+GLLSD ATRLCGSGDLF+ SRGPAFSFN+V KNFGLPLV
Sbjct: 571  KRWAEMNTRFCRDVRNFLRGEGLLSDLATRLCGSGDLFSDSRGPAFSFNYVTKNFGLPLV 630

Query: 1606 DLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDELG 1785
            DLVSFS  ELASELSWNCG EGPTN  +VLE RL+QIRNFLF+LF+SLGVPVLNMGDE G
Sbjct: 631  DLVSFSRDELASELSWNCGKEGPTNNNSVLEIRLRQIRNFLFLLFISLGVPVLNMGDECG 690

Query: 1786 YSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIVWY 1965
            +STGG     DR PIDW   +  F  QI +FI FLSS R+RR+DIFQ R FLKVENIVW 
Sbjct: 691  FSTGGLSSYGDRHPIDWNVTKSVFGKQITQFITFLSSLRNRRNDIFQRRDFLKVENIVWQ 750

Query: 1966 ASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPYQLEGNS 2145
             SN S P+W+DP+CKFLA+T++++ D   S+S  GDLF+CFNA D PE  ILP  LEG  
Sbjct: 751  GSNLSQPKWEDPSCKFLAVTIRADKDDNMSTSYKGDLFICFNADDFPEIAILPPLLEGYE 810

Query: 2146 WLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAER 2283
            W RLVDTSL FP FF   S       + L  YEL+PHSC LFE ++
Sbjct: 811  WFRLVDTSLPFPGFFSGES----VFTEALLGYELKPHSCVLFEVKK 852


>ref|XP_020700621.1| isoamylase 2, chloroplastic [Dendrobium catenatum]
 gb|PKU77045.1| Isoamylase 2, chloroplastic [Dendrobium catenatum]
          Length = 894

 Score =  897 bits (2318), Expect = 0.0
 Identities = 447/733 (60%), Positives = 542/733 (73%), Gaps = 8/733 (1%)
 Frame = +1

Query: 106  SHSLVAGV---KNECSSIVM--PFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVL 270
            SHS V      K E S  +M  PF+ DS  R+ L L+FD    PFYLSF L   SD    
Sbjct: 161  SHSSVLPTTKFKPEASQHLMKTPFVRDSC-RHILVLDFDQNDVPFYLSFFLQ--SDGTGQ 217

Query: 271  RREIRTHRKASFCLPVGLGPGYPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEP 450
             R IRTHRK +FC+PVGLGPGYP  LGVS S+DG  NF+LFSR+++ V+LC Y  KT EP
Sbjct: 218  ERTIRTHRKTNFCVPVGLGPGYPMALGVSSSNDGLANFSLFSRNAKSVVLCFYKSKTSEP 277

Query: 451  SLEIQLDPYVNRTGNIWHVSMESIAGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMV 624
            SLEI LDPYVN TG+IWHVS+ SI  Y+ YGYRCKG +  + GS  +   VL DPYAK +
Sbjct: 278  SLEIDLDPYVNHTGDIWHVSVHSITNYIGYGYRCKGDISWDGGSSSNAHHVLLDPYAKSI 337

Query: 625  QNLLPDLGESGIVSKFLGSLEKEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLP 804
             NL  D GES  + K LG L KEP F+W  D HP +P+E+L++YRLNV QFTK +S  L 
Sbjct: 338  GNLSSDQGESACMVKCLGYLRKEPNFDWNGDTHPQIPMEKLIIYRLNVRQFTKSESSELA 397

Query: 805  EDLAGTFIGVSEKVSHFKELGVNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTAS 984
             ++AGTF GV +KV H   LG+N+VLLEPVF F+ ++GPYFPYHFFS   +YG    + S
Sbjct: 398  INVAGTFAGVVKKVQHLINLGINAVLLEPVFSFDKKRGPYFPYHFFSPMSIYGGVDESIS 457

Query: 985  ATTSMKEMIKRLHVEGIEVLMEVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEA 1164
            +  SMKEM+K LH  GIEVL+EVVFTHT E  D+ SQ++SL GID+ SYY ++G +  E 
Sbjct: 458  SVYSMKEMVKILHSHGIEVLLEVVFTHTCEVGDSDSQIISLLGIDNPSYYIVDGNVHLEN 517

Query: 1165 NSLLKCYNPIVQHLIIDSLRRWVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFD 1344
             S LKC +PIVQ +I+DSL  WV +FH+DGFCFINSSS+ +G  G HL RPPLVE IAFD
Sbjct: 518  CSALKCNDPIVQQMILDSLHHWVTEFHVDGFCFINSSSLLQGSNGDHLPRPPLVEDIAFD 577

Query: 1345 PVLSKAKIIADCWSPIDMSYKEIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLC 1524
            P+LS+ KII+DCWSPIDMSYK   FPHW+ WA+MN+++  DVRNFLRG+GLLS  ATRLC
Sbjct: 578  PLLSETKIISDCWSPIDMSYKYAIFPHWQRWAEMNSKYFLDVRNFLRGQGLLSHLATRLC 637

Query: 1525 GSGDLF-ASRGPAFSFNFVAKNFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLET 1701
            GSGD+F ASRGPA+SFN++ KN GL LVDLVSFS  +LASELSWNCG EGPT K +VLE 
Sbjct: 638  GSGDIFTASRGPAYSFNYITKNAGLTLVDLVSFSGADLASELSWNCGVEGPTEKNSVLEI 697

Query: 1702 RLKQIRNFLFILFVSLGVPVLNMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFI 1881
            RL+Q+RNFLFILF+SLGVPV+NMGDE GYSTGG    ++R PIDW SL+  FS QI KF+
Sbjct: 698  RLRQVRNFLFILFISLGVPVINMGDECGYSTGGLPFYDNRVPIDWKSLRTGFSIQITKFV 757

Query: 1882 AFLSSFRSRRSDIFQSRHFLKVENIVWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSS 2061
            AFL+S R+RRSD+FQ + F K ENI WY SN S P+W+DP+CKFLAM LK+E D K+ +S
Sbjct: 758  AFLASLRNRRSDVFQRKIFYKAENITWYGSNLSLPKWEDPSCKFLAMELKAEKDVKSRNS 817

Query: 2062 RSGDLFVCFNASDCPETVILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSY 2241
             +GDLF+  NASD PE V LP    G  W RLVDTSL +P FF   S+P+  K  GLSSY
Sbjct: 818  YNGDLFITINASDLPELVTLPESSAGYVWFRLVDTSLPYPYFFSSDSNPNSQKNAGLSSY 877

Query: 2242 ELEPHSCALFEAE 2280
            EL+PH+CALFEA+
Sbjct: 878  ELKPHTCALFEAK 890


>gb|PKA66852.1| Isoamylase 2, chloroplastic [Apostasia shenzhenica]
          Length = 881

 Score =  874 bits (2257), Expect = 0.0
 Identities = 426/710 (60%), Positives = 530/710 (74%), Gaps = 3/710 (0%)
 Frame = +1

Query: 157  PFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGY 336
            P    SSG +T  LEF   + PFYLSFLL +    G  + EI+T RK  F +PVGLG G+
Sbjct: 174  PLTRGSSGMHTAVLEFSFQQIPFYLSFLLQVD---GAAKSEIKTERKTYFRVPVGLGSGH 230

Query: 337  PAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHVSME 516
              PLG+S+ DDG+ NF+LFSR++E V+LCLYDGKT EPS EI+LDPYVNRTG+IWHV + 
Sbjct: 231  SLPLGISLFDDGSANFSLFSRNAESVVLCLYDGKTNEPSFEIELDPYVNRTGDIWHVLLY 290

Query: 517  SIAGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEK 690
             ++ Y  YGYRCKG +  + GS +  + VL DPYAK +    PD G S  +SK LG L K
Sbjct: 291  DLSNYTRYGYRCKGKIFWDTGSRFHARHVLLDPYAKTIGEFCPDEGGSVSLSKCLGCLRK 350

Query: 691  EPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGV 870
             P F+W  D HP + +E+LVVYRLNVG+FTK+KS GLPE++AGTF GV++K+ H   LGV
Sbjct: 351  MPKFDWNGDTHPQISMEKLVVYRLNVGRFTKEKSSGLPENVAGTFAGVTKKIQHLTSLGV 410

Query: 871  NSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLME 1050
            N+VLLEP+F F+  + PYFPYHFFS  + YG      SA   MKEM+K LH  GIEVLME
Sbjct: 411  NAVLLEPIFPFDKNRNPYFPYHFFSPMNEYGDVDEFNSAVNLMKEMVKTLHTNGIEVLME 470

Query: 1051 VVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRW 1230
            VVFTHT E  DT S+++S RGID+SSYY +E    +  NS+L C +  VQ +I+DSL  W
Sbjct: 471  VVFTHTCEGNDTKSRIISFRGIDNSSYYIVEPNGASGINSMLNCNSAAVQQIILDSLHYW 530

Query: 1231 VVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKE 1410
            + +FH+DGFCFINSSS+ +G     L R PL+EAIAFDP+L+K KII+DCWSPID S K+
Sbjct: 531  LTEFHVDGFCFINSSSLLQGSNSDDLPRSPLIEAIAFDPLLTKTKIISDCWSPIDESCKD 590

Query: 1411 IQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFA-SRGPAFSFNFVAKN 1587
            + FPHW+ W +MN +F  DVRNFL+G+GLLS+FATRLCGSGD+F  SRGP++SFN+V KN
Sbjct: 591  VVFPHWRRWGEMNPKFSRDVRNFLKGEGLLSEFATRLCGSGDIFLHSRGPSYSFNYVTKN 650

Query: 1588 FGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLN 1767
            FGL LVDLVSFS  +LASELSWNCGDEGPT+K  VLE RL+QIRNFLFILF+SLGVPVLN
Sbjct: 651  FGLTLVDLVSFSGADLASELSWNCGDEGPTDKSIVLEIRLRQIRNFLFILFISLGVPVLN 710

Query: 1768 MGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKV 1947
            MGDE GYS GGS   ++R P+DW++L+  F  QI  FI+FL+SFR RRSD+FQ + FL++
Sbjct: 711  MGDECGYSAGGSPSYDERMPLDWSNLRTEFGRQITSFISFLTSFRDRRSDVFQRKEFLEL 770

Query: 1948 ENIVWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRSGDLFVCFNASDCPETVILPY 2127
            ENI W+ SNQS  +W+DP+CKFLAM LK+E D+ +S S +G+LF+ FNAS   E+  LP 
Sbjct: 771  ENIAWHGSNQSPLKWEDPSCKFLAMDLKAEEDAGSSDSGNGNLFISFNASADIESANLPG 830

Query: 2128 QLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEA 2277
              EG +W RLVDTSL +P FF    D DV K +GLSSYEL+PHSCALFEA
Sbjct: 831  LPEGQAWFRLVDTSLPYPGFFSSDFDADVCKFEGLSSYELKPHSCALFEA 880


>ref|XP_023886631.1| isoamylase 2, chloroplastic [Quercus suber]
 ref|XP_023886632.1| isoamylase 2, chloroplastic [Quercus suber]
 gb|POE68275.1| isoamylase 2, chloroplastic [Quercus suber]
          Length = 887

 Score =  864 bits (2232), Expect = 0.0
 Identities = 428/717 (59%), Positives = 538/717 (75%), Gaps = 10/717 (1%)
 Frame = +1

Query: 157  PFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGY 336
            PFI  S+GR+ L+LEF++ + PFYLSF+L L SDSG    EIR+HRK  FC+PVG   GY
Sbjct: 174  PFIQTSAGRFALELEFEAKQIPFYLSFILKLPSDSGSRGSEIRSHRKTKFCVPVGFRKGY 233

Query: 337  PAPLGVSISDDGTVNFALFSRSSEKVILCLYD-GKTEEPSLEIQLDPYVNRTGNIWHVSM 513
            PAPLG+S S DG++NFA+FSR+ E VILC YD  K EEP+LE+ LDPY+NR+G+IWH S 
Sbjct: 234  PAPLGLSFSPDGSMNFAIFSRNVEGVILCFYDDAKAEEPALELDLDPYINRSGDIWHASF 293

Query: 514  ESIAGYVNYGYRCKGMV--EKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLE 687
            ES   +V+YGY+CKG +     + +D  Q++ DPYAK++ N +P    S    K+LG L 
Sbjct: 294  ESAWTFVSYGYQCKGSLLQRNNNKFDAGQIILDPYAKIIVNSIPSSDGS---QKYLGRLC 350

Query: 688  KEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELG 867
            KEP F+WG+D HPYLP+E+LVVYRLNV  FT+ KS  L  D+AGTFIG++EK+ HFK+LG
Sbjct: 351  KEPGFDWGDDTHPYLPMEKLVVYRLNVRHFTEHKSSQLATDVAGTFIGLTEKLQHFKDLG 410

Query: 868  VNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLM 1047
            +N+VLLEP+F F+ + GPYFP HFFS ++LYG  G + SA  SMKEM+K+LH  GIEVL+
Sbjct: 411  MNAVLLEPIFSFDEKNGPYFPCHFFSPTNLYGPSGGSVSAINSMKEMVKKLHANGIEVLL 470

Query: 1048 EVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRR 1227
            EVVFTHT           SL+GID  SYY +      EA + L C  PIVQ LI+DSL+ 
Sbjct: 471  EVVFTHTAVAG-------SLQGIDDISYYHVNEGEDLEATNYLNCNYPIVQQLILDSLQY 523

Query: 1228 WVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYK 1407
            WV +FHIDGFCFIN+SS+ RG +G +LSRPPLVEAI+FDP+LSK KIIADCW P DM  K
Sbjct: 524  WVTEFHIDGFCFINASSLLRGFHGEYLSRPPLVEAISFDPLLSKTKIIADCWDPHDMLPK 583

Query: 1408 EIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVAK 1584
            E +FPHWK WA+++ +FC DVRNF RG+GLLSD ATRLCGSGD+F+  RGPAFSFNF+A+
Sbjct: 584  ETRFPHWKRWAEIDTKFCNDVRNFWRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNFIAR 643

Query: 1585 NFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVL 1764
            NFGL LVDLVSFS+  LAS+LSWNCG+EGPT+  TVLE RLKQIRNFLF+L++SLGVP+L
Sbjct: 644  NFGLTLVDLVSFSNDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPIL 703

Query: 1765 NMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLK 1944
            NMGDE G S GGS    DR+P+DW +L+  FS Q  +FI+FLSS R RRSD+ Q R+FLK
Sbjct: 704  NMGDECGQSCGGSPAYSDRKPLDWNTLKTGFSIQTSQFISFLSSLRMRRSDLLQKRNFLK 763

Query: 1945 VENIVWYASNQSHPQWDDPTCKFLAMTLK-----SELDSKTSSS-RSGDLFVCFNASDCP 2106
             ENI W+ S+QS P+W+DP+C+FLAMTL+     S L+S++SS  R GDLF+ FNA+D  
Sbjct: 764  EENIEWHGSDQSPPKWEDPSCRFLAMTLRADEVESSLNSESSSHVRRGDLFIAFNAADLS 823

Query: 2107 ETVILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEA 2277
            E+VILP   EG SW  LVDT+L FP FF  + +    +     +YE++ HSCALFEA
Sbjct: 824  ESVILPQPPEGMSWRCLVDTALPFPGFFSTSGETLPEQMAESVAYEMKSHSCALFEA 880


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  859 bits (2220), Expect = 0.0
 Identities = 444/782 (56%), Positives = 552/782 (70%), Gaps = 20/782 (2%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF--LSSFRNEVMLIYEISAYEFSHS---------LVAGVKNECSS 147
            V+VG +   Y V IE   L  + ++  LI     +  + S         LV  V +  + 
Sbjct: 112  VIVGKKNKKYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTE 171

Query: 148  IVMPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLG 327
            I  PF+  SSG + L L+F++  APFYLSFLL  + D+ +   +IR+HRK +FC+PVG G
Sbjct: 172  I--PFMERSSGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFG 229

Query: 328  PGYPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKTEEPSLEIQLDPYVNRTGNIWHV 507
             GYPAPLG+S S DG+ NFA FSR++  V+LCLYDG +++P+LEI LDPYVNRTG+IWH 
Sbjct: 230  RGYPAPLGLSFSSDGSPNFAFFSRNAGGVVLCLYDGTSDKPALEIDLDPYVNRTGDIWHA 289

Query: 508  SMESIAGYVNYGYRCK--GMVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSK-FLG 678
            SMES+  +V+YGYRCK   + + G     + V  DPYAK+++N   D  + G+  +  LG
Sbjct: 290  SMESVGSFVSYGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSD--DHGLKPQPRLG 347

Query: 679  SLEKEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFK 858
             L+KEP F W +D HPY+P+E+LVVYRLNV  FTKD+S  +  DLAGTF G+ EK+ HFK
Sbjct: 348  ELQKEPAFNWNDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFK 407

Query: 859  ELGVNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIE 1038
            +LGVN+VLLEP+F F+ +KGPYFP+HFFS  ++YG      S   S+KEM+KRLH  GIE
Sbjct: 408  DLGVNAVLLEPIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIE 467

Query: 1039 VLMEVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDS 1218
            VL+EVVFTHT E         +L+GID S YY + G       + L C   IVQ +I+DS
Sbjct: 468  VLLEVVFTHTAESG-------ALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDS 520

Query: 1219 LRRWVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDM 1398
            LR WV +FH+DGFCFIN+SS+ RG +G +LSRPPLVE IAFDP+LSK KIIADCW P +M
Sbjct: 521  LRYWVTEFHVDGFCFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNM 580

Query: 1399 SYKEIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNF 1575
              KEI+FPHWK WA++N RFC DVRNFLRG+GL SDFATRLCGSGD+F   RGPAFSFNF
Sbjct: 581  LPKEIRFPHWKRWAEVNTRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNF 639

Query: 1576 VAKNFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGV 1755
              KNFGLPLVDLVSFS  ELASELSWNCGDEGPTNK TVLE RLKQIRNFLFIL+VSLGV
Sbjct: 640  TTKNFGLPLVDLVSFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGV 699

Query: 1756 PVLNMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRH 1935
            P+LNMGDE G S+GGS    DR+P +W S++  F  Q  +FI+FLSS RSRRSD+ Q R 
Sbjct: 700  PILNMGDECGQSSGGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRS 759

Query: 1936 FLKVENIVWYASNQSHPQWDDPTCKFLAMTLKSE-----LDSKTSSSRSGDLFVCFNASD 2100
            FLK E+I W+ S+QS P+WDDP+ KFLAMTLK+E     L S+ SSS  GDLF+ FN +D
Sbjct: 760  FLKEESIDWHGSDQSPPRWDDPSSKFLAMTLKAENMEGQLPSE-SSSIKGDLFIAFNTAD 818

Query: 2101 CPETVILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAE 2280
                VILP    G  W RLVDT+L FP FF    +  + K  GL +Y++E HSCALFEA 
Sbjct: 819  RSVKVILPPPPTGMVWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEAN 878

Query: 2281 RI 2286
             +
Sbjct: 879  TL 880


>ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]
          Length = 883

 Score =  858 bits (2218), Expect = 0.0
 Identities = 437/780 (56%), Positives = 562/780 (72%), Gaps = 18/780 (2%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIE----FLSSFRNEVMLIYEISAYEFSHSLVAGVKNEC-----SSIV 153
            V V  +   Y+V+IE    +LSS    ++L + I   + S  +    K+       +++ 
Sbjct: 113  VFVRKRNAKYTVNIEVPSLYLSSSDRRLVLRWGIYRADSSCFVPLDFKSSTPNDTTTTLE 172

Query: 154  MPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPG 333
             PF   SSGR+T +LEF++ + PFY SF+L   +D+ V   EIR+HRK +FC+PVG G G
Sbjct: 173  TPFTQTSSGRFTFELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRG 232

Query: 334  YPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKT-EEPSLEIQLDPYVNRTGNIWHVS 510
            YP PLG++ S+DG++NFA+FSR++E V+LCLYD  T E+P+LE+ LDPYVNR+G+IWHVS
Sbjct: 233  YPGPLGLTFSNDGSMNFAIFSRNAESVVLCLYDDTTAEKPALELDLDPYVNRSGDIWHVS 292

Query: 511  MESIAGYVNYGYRCKG--MVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSL 684
              S   +V+YGY+ KG  ++   + +D   VL DPYAK++   +P+   +G+  K+LG L
Sbjct: 293  FGSARAFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL--KYLGRL 350

Query: 685  EKEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKEL 864
             +EP F+W  D  P L +E+LVVYRLNV +FT+ KS  LP D+ G+F G++EK+ HFK+L
Sbjct: 351  CEEPAFDWAGDVRPDLSMEKLVVYRLNVMRFTEHKSSQLPTDIGGSFSGLTEKLEHFKDL 410

Query: 865  GVNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVL 1044
            GVN+VLLEP+F F+ +KGPYFP HFFS  D +G  G   SA  SMKEM+++ H  GIEVL
Sbjct: 411  GVNAVLLEPIFPFDEQKGPYFPRHFFSPMDCFGPSGGPVSAVNSMKEMVRKFHANGIEVL 470

Query: 1045 MEVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLR 1224
            +EVVFTHT E         +L+GID SSYY +      EA + L C  P+VQ L++DSLR
Sbjct: 471  LEVVFTHTAEGE-------ALQGIDISSYYHVNEVEDLEARNALNCNYPVVQQLVLDSLR 523

Query: 1225 RWVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSY 1404
             WV +FH+DGFCFIN+SS+ RG  G +LSRPPLVEAIAFDP+LSK KIIAD W P  M+ 
Sbjct: 524  YWVTEFHVDGFCFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADRWDPHGMAP 583

Query: 1405 KEIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVA 1581
            KE +FPHWK WA++N +FC DVRNFLRG+GLLSD ATRLCG+GD+F+  RGPAF+FNF++
Sbjct: 584  KETRFPHWKRWAEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFIS 643

Query: 1582 KNFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPV 1761
            +N GLPLVDLVSFS  ELASELSWNCG+EGPTNK  VLE RLKQIRNFLFILFVSLGVPV
Sbjct: 644  RNSGLPLVDLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPV 703

Query: 1762 LNMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFL 1941
            LNMGDE G STGGS    DR+  DW +L+  F+TQ  +FIAFLSSFR+RRSD+ Q R+FL
Sbjct: 704  LNMGDECGQSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRNRRSDLLQKRNFL 763

Query: 1942 KVENIVWYASNQSHPQWDDPTCKFLAMTLKSELDSKT----SSSRS-GDLFVCFNASDCP 2106
            K ENI WY S+Q+ P+W+DP+CKFLAM LK++ D        SSRS GDLFV F+A+D  
Sbjct: 764  KEENIYWYESDQTPPRWEDPSCKFLAMRLKADEDEVNQPGDESSRSRGDLFVAFSAADHS 823

Query: 2107 ETVILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERI 2286
            ETV+LP  LEG  W  LVDT+L FP FF    +P V +  GL +YE++ HSCALFEA  +
Sbjct: 824  ETVVLPPPLEGMGWRCLVDTALPFPGFFSTDGEPVVEQMVGLFAYEMKSHSCALFEARSL 883


>ref|XP_007201750.1| isoamylase 2, chloroplastic [Prunus persica]
 gb|ONH93660.1| hypothetical protein PRUPE_8G244800 [Prunus persica]
          Length = 883

 Score =  850 bits (2195), Expect = 0.0
 Identities = 434/780 (55%), Positives = 554/780 (71%), Gaps = 18/780 (2%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEFLSSF--RNEVMLIYEISAYEFSHSLVAGVKNECSS-------IV 153
            V V  +   Y+V+IE  SS    N+  L+     Y    S    +  + S+       + 
Sbjct: 113  VFVRKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLE 172

Query: 154  MPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPG 333
             P I  SSGR+TL+LEF++ + PFY SF+L   +D+ V   EIR+HRK +FC+PVG G G
Sbjct: 173  TPLIQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRG 232

Query: 334  YPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKT-EEPSLEIQLDPYVNRTGNIWHVS 510
            YP PLG++ S+DG++NFA+FSR++E V LCLYD  T E+P+LE+ LDPYVNR+G+IWHVS
Sbjct: 233  YPGPLGLTFSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVS 292

Query: 511  MESIAGYVNYGYRCKG--MVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSL 684
              S   +V+YGY+ KG  ++   + +D   VL DPYAK++   +P+   +G+  K+LG L
Sbjct: 293  FGSAWSFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL--KYLGRL 350

Query: 685  EKEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKEL 864
             +EP F+W  D  P L +E+LVVYRLNV +FT+ KS  LP ++ G+F G++EK+ HFK+L
Sbjct: 351  CEEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDL 410

Query: 865  GVNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVL 1044
            GVN+VLLEP+F F+ +KGPYFP+HFFS  D +G      SA  SMKEM+++ H  GIEVL
Sbjct: 411  GVNAVLLEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVL 470

Query: 1045 MEVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLR 1224
            +EVVFTHT E         +L+GID SSYY +      EA + L C  PIVQ L++DSLR
Sbjct: 471  LEVVFTHTAEGE-------ALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLR 523

Query: 1225 RWVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSY 1404
             WV +FH+DGF FIN+SS+ RG  G +LSRPPLVEAIAFDP+LSK KIIADCW P  M+ 
Sbjct: 524  YWVTEFHVDGFFFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAP 583

Query: 1405 KEIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVA 1581
            KE  FPHW+ WA++N +FC DVRNFLRG+GLLSD ATRLCG+GD+F+  RGPAF+FNF++
Sbjct: 584  KETHFPHWRRWAEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFIS 643

Query: 1582 KNFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPV 1761
            +N GLPLVDLVSFS  ELASELSWNCG+EGPTNK  VLE RLKQIRNFLFILFVSLGVPV
Sbjct: 644  RNSGLPLVDLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPV 703

Query: 1762 LNMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFL 1941
            LNMGDE G STGGS    DR+  DW +L+  F+TQ  +FIAFLSSFR RRSD+ Q R+FL
Sbjct: 704  LNMGDECGQSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFL 763

Query: 1942 KVENIVWYASNQSHPQWDDPTCKFLAMTLKSELD-----SKTSSSRSGDLFVCFNASDCP 2106
            K ENI WY S+Q+ P+W+DP+ KFLAM LK++ D        SS   GDLFV F+A+D  
Sbjct: 764  KEENIGWYESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHS 823

Query: 2107 ETVILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERI 2286
            ETV+LP  LEG  W RLVDT+L FP FF    +P V +  GL +YE++ HSCALFEA  +
Sbjct: 824  ETVVLPPPLEGMGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARSL 883


>gb|OMP04997.1| hypothetical protein COLO4_09141 [Corchorus olitorius]
          Length = 868

 Score =  848 bits (2192), Expect = 0.0
 Identities = 428/767 (55%), Positives = 541/767 (70%), Gaps = 8/767 (1%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF--LSSFRNEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIWDS 174
            + V  +   Y V IE   L    N   L+     Y   H +      + ++I  PFI  S
Sbjct: 113  IFVRKKSVKYVVDIEVSSLQLSGNNNKLVLSGGVYRSDHDI------KTNNIEAPFIARS 166

Query: 175  SGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPLGV 354
            S    L+LEF++ +APFYLSFLL  SSD+     EIR+HRK SFC+P+G   GYP PLG+
Sbjct: 167  SSELALELEFEAKEAPFYLSFLLKASSDANSTGLEIRSHRKTSFCVPIGFDQGYPVPLGL 226

Query: 355  SISDDGTVNFALFSRSSEKVILCLYDGK-TEEPSLEIQLDPYVNRTGNIWHVSMESIAGY 531
            S S+DG++NFA++SR++E ++LCLYD   +E+P+LE+ LDPYVNRTG+IWH S+E    +
Sbjct: 227  SFSNDGSMNFAVYSRNAESLVLCLYDDTGSEKPALELDLDPYVNRTGDIWHASLEGAWTF 286

Query: 532  VNYGYRCKGMVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTFEWG 711
            V+YGYRCKG    G  +  ++VL DPYAK++ + +P+  ESG++ K LG L KEP F+W 
Sbjct: 287  VSYGYRCKG---DGDAFHAERVLLDPYAKIIGSSIPNHYESGLLLKHLGRLCKEPAFDWS 343

Query: 712  NDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVLLEP 891
             D  P LPLE+LVVYRLNV +FT+DKS  LP D+AGTF GV+EKV H K LG+N+VLLEP
Sbjct: 344  GDVCPNLPLEKLVVYRLNVMRFTEDKSSKLPADVAGTFSGVTEKVQHLKYLGINAVLLEP 403

Query: 892  VFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFTHTG 1071
            +F F+ +KGPYFP HFFS + LYG    + SA  S+K M+K LH  GIEVL+EVVFTHT 
Sbjct: 404  IFTFDEQKGPYFPCHFFSPTSLYGPSNGSVSAINSIKGMVKNLHANGIEVLLEVVFTHTA 463

Query: 1072 EETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDFHID 1251
            E         +L+G+D  SYY        E  + L C  P+VQ +I+DSLR WV +FHID
Sbjct: 464  EGG-------ALQGLDDESYYYRNRVEDLEEKNALNCNYPVVQQMILDSLRHWVTEFHID 516

Query: 1252 GFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFPHWK 1431
            GFCFIN+S + RG +G HLSRPPLVEAIAFDP+LSK K+IADCW P +M  KEI FPHWK
Sbjct: 517  GFCFINASCLLRGFHGEHLSRPPLVEAIAFDPLLSKTKVIADCWDPHEMMPKEIHFPHWK 576

Query: 1432 IWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVAKNFGLPLVD 1608
             WA+MN +FC D+RNFLRG+  LS  ATRLCGSGD+F+  RGPAFSFNF+A+NFGLPLVD
Sbjct: 577  RWAEMNTKFCSDIRNFLRGEDALSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVD 636

Query: 1609 LVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDELGY 1788
            LVSFS+ ELASELSWNCG+EGPT+   VLE RLKQIRN++FILFVSLGVPVLNMGDE G 
Sbjct: 637  LVSFSNAELASELSWNCGEEGPTSNTAVLERRLKQIRNYIFILFVSLGVPVLNMGDECGQ 696

Query: 1789 STGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIVWYA 1968
            S+GGS     R+ +DW ++   F  Q  +FI+FLSS R RRSD+ Q R+FLK ENI W+ 
Sbjct: 697  SSGGSLSYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRERRSDLLQRRNFLKEENIEWHG 756

Query: 1969 SNQSHPQWDDPTCKFLAMTLKSE----LDSKTSSSRSGDLFVCFNASDCPETVILPYQLE 2136
            S+QS P+W+DP+CKFLAMTLK++    L S  +S   GDLF+  NA D  E +ILP   E
Sbjct: 757  SSQSQPEWEDPSCKFLAMTLKADKAESLLSSEASELKGDLFIAINADDKTENIILPPCPE 816

Query: 2137 GNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEA 2277
            G +W RLVDT+L +P FFL      + +  GL +YE++  SC LFEA
Sbjct: 817  GMAWRRLVDTALPYPGFFLADGKAVLEQMAGLVAYEMKSRSCTLFEA 863


>ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prunus mume]
          Length = 883

 Score =  843 bits (2177), Expect = 0.0
 Identities = 431/780 (55%), Positives = 555/780 (71%), Gaps = 18/780 (2%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIE----FLSSFRNEVMLIYEISAYEFSHSLVAGVKNEC-----SSIV 153
            V V  +   Y V+IE    +LSS    ++L + +   + S  +    K+       +++ 
Sbjct: 113  VFVRKRNAKYIVNIEVPSLYLSSSDRRLVLHWGMYRADSSCFMPLDFKSSTPNDTTTTLE 172

Query: 154  MPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPG 333
             PFI  SSGR+TL+LEF++ + PFY SF+L   +D+ V   EIR+HRK +FC+PVG G G
Sbjct: 173  TPFIQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRG 232

Query: 334  YPAPLGVSISDDGTVNFALFSRSSEKVILCLYDGKT-EEPSLEIQLDPYVNRTGNIWHVS 510
            YP PLG++ S+DG++NFA+FSR++E V+LCLYD  T E+P+LE+ LDPYVNR+G+IWHVS
Sbjct: 233  YPGPLGLTFSNDGSMNFAIFSRNAESVVLCLYDNTTAEKPALELDLDPYVNRSGDIWHVS 292

Query: 511  MESIAGYVNYGYRCKG--MVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSL 684
              S   +V+YGY+ K   ++   + +D   VL DPYAK++   +P+   +G+  K+LG L
Sbjct: 293  FGSAWTFVSYGYKFKRNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL--KYLGRL 350

Query: 685  EKEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKEL 864
             +EP F+W  D  P L +E+LVVYRLNV +FT+ KS  LP ++ G+F G++EK+ HFK+L
Sbjct: 351  CEEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDL 410

Query: 865  GVNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVL 1044
            GVN+VLLEP+F F+ +KGPYFP HFFS  +  G      SA  SMKEM+++ H  GIEVL
Sbjct: 411  GVNAVLLEPIFPFDEQKGPYFPRHFFSPMECIGPSRGPVSAVNSMKEMVRKFHANGIEVL 470

Query: 1045 MEVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLR 1224
            +EVVFTHT E         +L+GID SSYY +      EA + L C  PIVQ L++DSLR
Sbjct: 471  LEVVFTHTAEGE-------ALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLR 523

Query: 1225 RWVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSY 1404
             WV +FHIDGFCFIN+SS+ RG  G +LSRPPLVEAIAFDP+LSK KIIADCW P  M+ 
Sbjct: 524  YWVTEFHIDGFCFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAP 583

Query: 1405 KEIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVA 1581
            KE +FPHWK WA++N +FC DVRN+LRGKGLLSD ATRLCG+GD+F+  RGPAF+FNF++
Sbjct: 584  KETRFPHWKRWAEVNTKFCNDVRNYLRGKGLLSDLATRLCGNGDIFSDGRGPAFAFNFIS 643

Query: 1582 KNFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPV 1761
            +N GLPLVDLVSFS  ELASELSWNCG+EGPTNK  VLE RLKQIRNFLFILFVSLGVPV
Sbjct: 644  RNSGLPLVDLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPV 703

Query: 1762 LNMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFL 1941
            LN GDE G STGGS    DR+  DW +L+  F+TQ  +FIAFLSSFR RRSD+ Q R+FL
Sbjct: 704  LNTGDECGQSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFL 763

Query: 1942 KVENIVWYASNQSHPQWDDPTCKFLAMTLKSELD-----SKTSSSRSGDLFVCFNASDCP 2106
            K ENI WY S+Q+ P+W+DP+ KFLAM LK++ D        SS   GDLF  F+A+D  
Sbjct: 764  KEENIDWYESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFAAFSAADHS 823

Query: 2107 ETVILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERI 2286
            ET++LP   EG  W RLVDT+L FP FF    +P V +  GL +YE++ HSCALFEA  +
Sbjct: 824  ETLVLPPPREGMGWRRLVDTALPFPGFFSTDGEPVVRQMVGLFAYEMKSHSCALFEARSL 883


>gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  841 bits (2173), Expect = 0.0
 Identities = 428/767 (55%), Positives = 540/767 (70%), Gaps = 8/767 (1%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF--LSSFRNEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIWDS 174
            V V  +  N+ V IE   L    +E  L+     +   H     +KN    I  PFI  S
Sbjct: 112  VFVRKRSVNHVVDIEVSSLQLSGDESQLVLSGGIHRSDHD----IKNRI--IETPFIAKS 165

Query: 175  SGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPLGV 354
            S    L+LEF++ +APFY SFLL   S + +   EIRTHRK +FC+PVG   GYPAPLG+
Sbjct: 166  SSELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLGL 225

Query: 355  SISDDGTVNFALFSRSSEKVILCLYDGK-TEEPSLEIQLDPYVNRTGNIWHVSMESIAGY 531
            S S DG++NFA++SR++E ++LCLYD   +E+P+LE+ LDP+VNRTG+IWH S+E    +
Sbjct: 226  SFSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTF 285

Query: 532  VNYGYRCKGMVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTFEWG 711
            V YGYRCKG  +    ++ ++VL DPYA+++ + +P+  ESG++ K LG L KEP F+W 
Sbjct: 286  VRYGYRCKGDTD---AFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWS 342

Query: 712  NDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVLLEP 891
            +D  P LP+E+LVVYRLNV  FT+DKS  LP D+ GTF GV+EKV H K+LGVN+VLLEP
Sbjct: 343  SDVCPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEP 402

Query: 892  VFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFTHTG 1071
            +F F+ +KGPYFP HFFS ++LYG    + SA  S+KEM+K LH  G+EVL+EVVFTHT 
Sbjct: 403  IFTFDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTA 462

Query: 1072 EETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDFHID 1251
            E         +L+G+D  SYY        E  + L C  P+VQ +I+DSLR WV +FHID
Sbjct: 463  EGG-------ALQGLDDLSYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHID 515

Query: 1252 GFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFPHWK 1431
            GFCFIN+S + RG YG HLSRPPL+EAIAFDP+LSK KIIADCW P DM  KEI+FPHWK
Sbjct: 516  GFCFINASCLLRGFYGEHLSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWK 575

Query: 1432 IWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVAKNFGLPLVD 1608
             WA+MN +FC D+RNFLRG+G LS  ATRLCGSGD+F+  RGPAFSFN++AKNFGLPLVD
Sbjct: 576  KWAEMNTKFCTDIRNFLRGEGALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVD 635

Query: 1609 LVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDELGY 1788
            LVSFS  E+ASELSWNCG EGPTNK  VLE RLKQIRNFLFILF+SLGVPVLNMGDE G 
Sbjct: 636  LVSFSKAEIASELSWNCGVEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQ 695

Query: 1789 STGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIVWYA 1968
            S+GGS     R+ +DW ++   F  Q  +FI+FLSS R RRSD+ Q R FLK ENI WY 
Sbjct: 696  SSGGSLSYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYG 755

Query: 1969 SNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRS----GDLFVCFNASDCPETVILPYQLE 2136
            S+QS P W+DP+CKFLAMTLK++ +    SS +    GDL +  NA+D  E +ILP   E
Sbjct: 756  SDQSPPGWEDPSCKFLAMTLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPE 815

Query: 2137 GNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEA 2277
            G +W RLVDT+L +P FF       + +  GL +YE++  SC LFEA
Sbjct: 816  GLAWRRLVDTALPYPGFFSADGKAVLEQMMGLFAYEMKSLSCTLFEA 862


>ref|XP_024168065.1| isoamylase 2, chloroplastic [Rosa chinensis]
 ref|XP_024168066.1| isoamylase 2, chloroplastic [Rosa chinensis]
 gb|PRQ26163.1| putative isoamylase [Rosa chinensis]
          Length = 870

 Score =  841 bits (2173), Expect = 0.0
 Identities = 423/775 (54%), Positives = 557/775 (71%), Gaps = 13/775 (1%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF----LSSFRNEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIW 168
            V V +   NYSV++E     LSS  + ++L + +   + S  + +        I  PFI 
Sbjct: 111  VFVRNNTVNYSVYVEVSSLQLSSSADRLVLSWGMYRADSSSLMPSDF------IETPFIE 164

Query: 169  DSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPL 348
             SSG +TL+LEF++ + PFYLSF++   +D+ +   EIR+HRK +FC+PVG G GYP PL
Sbjct: 165  TSSGSFTLELEFEAKQTPFYLSFIVKSLADANLSGLEIRSHRKTNFCVPVGFGRGYPTPL 224

Query: 349  GVSISDDGTVNFALFSRSSEKVILCLYDGKT-EEPSLEIQLDPYVNRTGNIWHVSMESIA 525
            G+S S DG++NFA+FSR++E V+LCL D  T ++P+LE+ LDPYVNR+G+IWHVS ES  
Sbjct: 225  GLSFSSDGSINFAIFSRNAESVVLCLDDDPTAQKPALELDLDPYVNRSGDIWHVSFESAW 284

Query: 526  GYVNYGYRCKG-MVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTF 702
             +V+YGYR KG ++   + +D   +L DPYAK++   +P+    G  SK +G L +EP F
Sbjct: 285  TFVSYGYRFKGTLLRNTNSFDEGDILLDPYAKVIDESIPN--NKGTGSKLVGRLCEEPVF 342

Query: 703  EWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVL 882
            +W  D  P+LP+E+L+VYRLN+ +FT+ KS  LP ++AGTF G++EK+  FK+LGVN++L
Sbjct: 343  DWDGDVRPHLPIEKLMVYRLNLTRFTEHKSSKLPTNVAGTFSGLTEKLDQFKDLGVNAIL 402

Query: 883  LEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFT 1062
            LEP+F F+ EKGPYFP HFFS  + +G  G   +   SMKEM+K  H  GIEVL+EVVFT
Sbjct: 403  LEPIFPFDEEKGPYFPCHFFSPMNCFGPSGGPIATINSMKEMVKEFHANGIEVLLEVVFT 462

Query: 1063 HTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDF 1242
            HT        +VL  +GID+SSYY        EA + L C +PIVQ +++DSLR WV +F
Sbjct: 463  HTA-----AGEVL--QGIDTSSYYHASTAKDLEARNSLNCNHPIVQQMVLDSLRYWVTEF 515

Query: 1243 HIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFP 1422
            H+DGFCFIN+SS+ RG  G + SRPPLVEAIAFDP+L+K K IADCW P +M  KE +FP
Sbjct: 516  HVDGFCFINASSLLRGVNGEYQSRPPLVEAIAFDPLLTKTKFIADCWDPHEMVPKETRFP 575

Query: 1423 HWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVAKNFGLP 1599
            HWK WA++N RFC DVRNFLRG+GLLSD ATRLCG+GD+F+  RGPAFSFNF+++N GLP
Sbjct: 576  HWKRWAEVNARFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFSFNFISRNSGLP 635

Query: 1600 LVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDE 1779
            LVDLVSFS  ELASELSWNCG+EGPT+K  VLE RLKQIRNFLFIL++SLGVPVLNMGDE
Sbjct: 636  LVDLVSFSGSELASELSWNCGEEGPTSKTAVLERRLKQIRNFLFILYLSLGVPVLNMGDE 695

Query: 1780 LGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIV 1959
             G S+GGS    DR+  DW +++  F+TQ ++FIA+LSSFR RRSD+ Q +H+ K ENI 
Sbjct: 696  CGQSSGGSPAYSDRKSFDWNAMETGFATQTRQFIAYLSSFRRRRSDLLQKKHYFKEENID 755

Query: 1960 WYASNQSHPQWDDPTCKFLAMTLKSELD------SKTSSSRSGDLFVCFNASDCPETVIL 2121
            WY S+QS P+W+DP CKFLAM LK++ D      +   +   GDLFV F+A++  ETVIL
Sbjct: 756  WYGSDQSLPRWEDPLCKFLAMRLKADQDEVENQLTNEYADLRGDLFVAFSAAEQSETVIL 815

Query: 2122 PYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERI 2286
            P   EG +W RLVDT+L FP FF    +P + + +GL SY ++ HSCALFEA R+
Sbjct: 816  PPPPEGMAWRRLVDTALPFPGFFSIDGEPIIEQMEGLFSYAMKSHSCALFEARRL 870


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  841 bits (2172), Expect = 0.0
 Identities = 431/779 (55%), Positives = 547/779 (70%), Gaps = 16/779 (2%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF----LSSFRNEVMLIYEISAYEFSHSLVAGVKNEC-----SSIV 153
            V+V  +   YSV++E     L S  +++++ + I   + S  +   +++       S++ 
Sbjct: 100  VLVRQKNLKYSVYVEVSSLKLKSGEDKLVMNWGIFRSDSSQFIPLDLQSSTPDDRSSTVE 159

Query: 154  MPFIWDSSGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPG 333
             PF+ +S G+  ++L F+    PFYLSFLL   SD      ++R+HRK SFC+PVGL  G
Sbjct: 160  TPFVQNSLGKLVIELNFEGSLTPFYLSFLLKFPSDGDSKSSDLRSHRKTSFCVPVGLASG 219

Query: 334  YPAPLGVSISDDGTVNFALFSRSSEKVILCLYDG-KTEEPSLEIQLDPYVNRTGNIWHVS 510
            YPAPLG+S S DG++NFALFS+S++ V+LCLYD  K E+P+LEI LDPYVNR+G+IWHVS
Sbjct: 220  YPAPLGLSFSADGSINFALFSQSADGVVLCLYDDTKAEKPTLEIDLDPYVNRSGDIWHVS 279

Query: 511  MESIAGYVNYGYRCKGMVE-KGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLE 687
            M+S   +V+YGYRCKG +  KG     + VL DPYAK++ N       SG V K LG L 
Sbjct: 280  MDSALPFVSYGYRCKGGIHGKGVKDHSEHVLLDPYAKVIAN-----AHSGSVLKTLGKLI 334

Query: 688  KEPTFEWGNDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELG 867
            KEPTF+W  D  P +P+E+LVVYR+NV  FTK KS GLP D+AG+F G++EK+ H K+LG
Sbjct: 335  KEPTFDWSGDIRPCIPMEKLVVYRVNVAHFTKHKSSGLPNDVAGSFTGITEKLQHCKDLG 394

Query: 868  VNSVLLEPVFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLM 1047
            VN++LLEPVF F+ +KGPYFP+HFFS   LYG     ++   SMKEM+K LH  GIEVL+
Sbjct: 395  VNAILLEPVFPFDEKKGPYFPFHFFSPMKLYGPSDDPSATVNSMKEMVKILHANGIEVLL 454

Query: 1048 EVVFTHTGEETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRR 1227
            E+VFTHT E         SLR ID SSYY I G     A + L C  PIVQ +I+DSLR 
Sbjct: 455  EIVFTHTAESC-------SLREIDKSSYY-ITGDEDLGAKNALNCNYPIVQQMILDSLRY 506

Query: 1228 WVVDFHIDGFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYK 1407
            W ++FH+DGFCFIN+SS+ +G +G  LSRPPLVEAIAFDP+LSK K+IAD W P DM+  
Sbjct: 507  WAIEFHVDGFCFINASSLLKGFHGEFLSRPPLVEAIAFDPLLSKTKLIADSWDPHDMASD 566

Query: 1408 EIQFPHWKIWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVAK 1584
            EI FPHWK WA++N  FC DVRNFLRG+GLLS+ ATRLCGSGD+F + RGP FSFN++ +
Sbjct: 567  EILFPHWKRWAEVNTNFCGDVRNFLRGEGLLSNLATRLCGSGDMFLNGRGPGFSFNYITR 626

Query: 1585 NFGLPLVDLVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVL 1764
            N GLPLVDLVSFS   LASELSWNCG+EGPTN   VLE RLKQIRN+LFILFVS GVPVL
Sbjct: 627  NSGLPLVDLVSFSSSNLASELSWNCGEEGPTNNTAVLERRLKQIRNYLFILFVSFGVPVL 686

Query: 1765 NMGDELGYSTGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLK 1944
            NMGDE G S+GGS    DR+P DW +L+  F  Q  +FI+FLSS R RRSD+ Q R FLK
Sbjct: 687  NMGDECGQSSGGSPAYGDRKPYDWNALRTGFGIQTTQFISFLSSLRVRRSDLLQKRSFLK 746

Query: 1945 VENIVWYASNQSHPQWDDPTCKFLAMTLKSELDSKTSSSR----SGDLFVCFNASDCPET 2112
             ENI WY S+QS P WDD + +FLAMTLK E +   SSS     +GDLFV FN++D  E 
Sbjct: 747  EENIDWYGSDQSLPTWDDRSSRFLAMTLKVEREENESSSEFSSLNGDLFVAFNSADQSER 806

Query: 2113 VILPYQLEGNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEAERIT 2289
            VILP       WLRLVDT++ FP FF    +P + +  GL +YE++ HSC LFEA++++
Sbjct: 807  VILPPPPPEMVWLRLVDTAVPFPGFFSRDGEPVLEQMAGLVTYEMKSHSCVLFEAKKLS 865


>ref|XP_017978673.1| PREDICTED: isoamylase 2, chloroplastic [Theobroma cacao]
          Length = 867

 Score =  841 bits (2172), Expect = 0.0
 Identities = 427/767 (55%), Positives = 538/767 (70%), Gaps = 8/767 (1%)
 Frame = +1

Query: 1    VVVGSQGGNYSVHIEF--LSSFRNEVMLIYEISAYEFSHSLVAGVKNECSSIVMPFIWDS 174
            V+V  +  N+ V IE   L    +E  L+     +   H     +KN    I  PFI  S
Sbjct: 112  VLVRKRSVNHVVDIEVSSLQLSGDESQLVLSGGVHRSDHD----IKNRI--IETPFIAKS 165

Query: 175  SGRYTLDLEFDSGKAPFYLSFLLHLSSDSGVLRREIRTHRKASFCLPVGLGPGYPAPLGV 354
            S    L+LEF++ +APFY SFLL   S +     EIRTHRK +FC+P+G   GYPAPLG+
Sbjct: 166  SSELALELEFEAKEAPFYFSFLLKAPSGANSSGSEIRTHRKTNFCVPIGFNQGYPAPLGL 225

Query: 355  SISDDGTVNFALFSRSSEKVILCLYDGK-TEEPSLEIQLDPYVNRTGNIWHVSMESIAGY 531
            S S DG++NFA++SR++E ++LCLYD   +E+P+LE+ LDP+VNRTG+IWH S+E    +
Sbjct: 226  SFSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTF 285

Query: 532  VNYGYRCKGMVEKGSGYDVQQVLFDPYAKMVQNLLPDLGESGIVSKFLGSLEKEPTFEWG 711
            V YGYRCKG  +    ++ ++VL DPYA+++ + +P+  ESG++ K LG L KEP F+W 
Sbjct: 286  VRYGYRCKGYTD---AFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWS 342

Query: 712  NDAHPYLPLEELVVYRLNVGQFTKDKSCGLPEDLAGTFIGVSEKVSHFKELGVNSVLLEP 891
             D  P LP+E+LVVYRLNV  FT+DKS  LP D+ GTF GV+EKV H K+LGVN+VLLEP
Sbjct: 343  GDVCPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEP 402

Query: 892  VFLFNVEKGPYFPYHFFSVSDLYGQDGSTASATTSMKEMIKRLHVEGIEVLMEVVFTHTG 1071
            +F F+ +KGPYFP HFFS ++LYG    + SA  S+KEM+K LH  G+EVL+EVVFTHT 
Sbjct: 403  IFTFDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTA 462

Query: 1072 EETDTGSQVLSLRGIDSSSYYDIEGKIGTEANSLLKCYNPIVQHLIIDSLRRWVVDFHID 1251
            E          L+G+D  SYY        E  + L C  P+VQ +I+DSLR WV +FHID
Sbjct: 463  EGG-------GLQGLDDLSYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHID 515

Query: 1252 GFCFINSSSMARGEYGVHLSRPPLVEAIAFDPVLSKAKIIADCWSPIDMSYKEIQFPHWK 1431
            GFCFIN+S + RG YG HLSRPPL+EAIAFDP+LSK KIIADCW P DM  KEI+FPHWK
Sbjct: 516  GFCFINASCLLRGFYGEHLSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWK 575

Query: 1432 IWAQMNNRFCFDVRNFLRGKGLLSDFATRLCGSGDLFAS-RGPAFSFNFVAKNFGLPLVD 1608
             WA+MN +FC D+RNFLRG+G LS  ATRLCGSGD+F+  RGPAFSFN++AKNFGLPLVD
Sbjct: 576  KWAEMNTKFCTDIRNFLRGEGALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVD 635

Query: 1609 LVSFSDPELASELSWNCGDEGPTNKKTVLETRLKQIRNFLFILFVSLGVPVLNMGDELGY 1788
            LVSFS  E+ASELSWNCG EGPTNK  VLE RLKQIRNFLFILF+SLGVPVLNMGDE G 
Sbjct: 636  LVSFSKAEIASELSWNCGVEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQ 695

Query: 1789 STGGSRLPEDRQPIDWASLQRSFSTQIKKFIAFLSSFRSRRSDIFQSRHFLKVENIVWYA 1968
            S+GGS     R+ +DW ++   F  Q  +FI+FLSS R RRSD+ Q R FLK ENI WY 
Sbjct: 696  SSGGSLSYGSRKLLDWNAMTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYG 755

Query: 1969 SNQSHPQWDDPTCKFLAMTLKSELDSKTSSSRS----GDLFVCFNASDCPETVILPYQLE 2136
            S+QS P W+DP+CKFLAMTLK++ +    SS +    GDL +  NA+D  E +ILP   E
Sbjct: 756  SDQSPPGWEDPSCKFLAMTLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPE 815

Query: 2137 GNSWLRLVDTSLAFPRFFLDTSDPDVHKAKGLSSYELEPHSCALFEA 2277
            G +W RLVDT+L +P FF       + +  GL +YE++  SC LFEA
Sbjct: 816  GLAWRRLVDTALPYPGFFSADGKAVLEQMMGLFAYEMKSLSCTLFEA 862