BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cheilocostus21_contig00005758
         (3270 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_010918919.1| PREDICTED: pullulanase 1, chloroplastic isof...  1455   0.0  
ref|XP_010918920.1| PREDICTED: pullulanase 1, chloroplastic isof...  1419   0.0  
ref|XP_020084106.1| pullulanase 1, chloroplastic [Ananas comosus]    1411   0.0  
ref|XP_020247136.1| LOW QUALITY PROTEIN: pullulanase 1, chloropl...  1335   0.0  
ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isof...  1322   0.0  
ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Que...  1321   0.0  
ref|XP_018808475.1| PREDICTED: pullulanase 1, chloroplastic isof...  1321   0.0  
ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isof...  1321   0.0  
ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isof...  1320   0.0  
ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Que...  1319   0.0  
gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]                1319   0.0  
gb|ONK57269.1| uncharacterized protein A4U43_C10F18340 [Asparagu...  1318   0.0  
ref|XP_018808474.1| PREDICTED: pullulanase 1, chloroplastic isof...  1317   0.0  
ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica...  1316   0.0  
gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]  1312   0.0  
gb|PHT29890.1| Pullulanase 1, chloroplastic [Capsicum baccatum]      1304   0.0  
ref|XP_019236141.1| PREDICTED: pullulanase 1, chloroplastic isof...  1304   0.0  
ref|XP_019236140.1| PREDICTED: pullulanase 1, chloroplastic isof...  1304   0.0  
ref|XP_020703866.1| pullulanase 1, chloroplastic [Dendrobium cat...  1303   0.0  
ref|XP_016436612.1| PREDICTED: pullulanase 1, chloroplastic-like...  1301   0.0  

>ref|XP_010918919.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Elaeis
            guineensis]
          Length = 965

 Score = 1455 bits (3766), Expect = 0.0
 Identities = 696/905 (76%), Positives = 790/905 (87%), Gaps = 1/905 (0%)
 Frame = +1

Query: 130  IPFCSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIG-DDESSVWLFASRKAA 306
            I  C    A ME TA+ +  ++ + LSYARAYWV+R  IAWNI  DD++ V+L+ASRKA+
Sbjct: 60   ITSCFPVPAAMERTAIAESSLSQETLSYARAYWVSRILIAWNINYDDDAYVYLYASRKAS 119

Query: 307  LFLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVA 486
            + L +E I+GYDVKI L  E  GLP NVT+KFPHI  Y AF++   +D ENLLKCQLA+A
Sbjct: 120  ISLTEEGIEGYDVKILLEPEYYGLPANVTEKFPHIVNYSAFKISESVDFENLLKCQLAIA 179

Query: 487  SFSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPS 666
             F  DGKC NAT LQ+ GVLDD+FAY+GPLGA+FSKE VTLHLWAPTAQEV+VC +++PS
Sbjct: 180  -FYVDGKCKNATGLQLPGVLDDLFAYNGPLGAVFSKEAVTLHLWAPTAQEVSVCIYKEPS 238

Query: 667  SGDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSN 846
             G+ L+ V+LEE  GVWSAVG +SWEG YY+YEV VYHP+T QIE CT  DPYARGLSSN
Sbjct: 239  GGEPLESVQLEEFGGVWSAVGPKSWEGCYYVYEVSVYHPSTSQIEKCTVTDPYARGLSSN 298

Query: 847  GRRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGY 1026
            G+RTWL+NLD ENLKP+GWD LA+EKP+LLS SDISIYELHIRDFSA+D +V+ D +GGY
Sbjct: 299  GKRTWLINLDSENLKPEGWDKLADEKPDLLSFSDISIYELHIRDFSASDHTVNCDIQGGY 358

Query: 1027 LAFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDK 1206
            LAFTSQ+S GICHLKKLSNAGLTH+HLLPSFHFAGVDDDKSNWK  D+ EMAKLP+DSDK
Sbjct: 359  LAFTSQESAGICHLKKLSNAGLTHVHLLPSFHFAGVDDDKSNWKYLDDGEMAKLPADSDK 418

Query: 1207 QQAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLD 1386
            QQAEV  +Q+EDGYNWGYNPV+WGVPKGSYA NPNGP+RILEFRKM+QA+NRIGLRVVLD
Sbjct: 419  QQAEVIGIQNEDGYNWGYNPVLWGVPKGSYAGNPNGPSRILEFRKMIQAVNRIGLRVVLD 478

Query: 1387 VVYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLT 1566
            VVYNHLA+SGP +ENSVLDK+VPGYYLRRNTDG IENS CMNNTASEHFMVERLILDDL 
Sbjct: 479  VVYNHLASSGPLNENSVLDKVVPGYYLRRNTDGAIENSACMNNTASEHFMVERLILDDLL 538

Query: 1567 SWAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVAN 1746
            +WAV YKVDGFRFDLMGHIMK TMVKA+  LQSLSKDK GVDG  IY+YGEGWDFGE+A 
Sbjct: 539  NWAVVYKVDGFRFDLMGHIMKSTMVKARSTLQSLSKDKDGVDGPKIYIYGEGWDFGEIAK 598

Query: 1747 NKRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSV 1926
            NKRGINASQFN+ GTGIGSFNDR+RDAVLGGSPFG+PLQQGF TGLSLEPNGYD GDKSV
Sbjct: 599  NKRGINASQFNISGTGIGSFNDRMRDAVLGGSPFGHPLQQGFVTGLSLEPNGYDHGDKSV 658

Query: 1927 MDFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVS 2106
             D MLS+SADHIQVGLAANL+D++LT H G  MKGSEV+THDG  VGY LSP E VNYVS
Sbjct: 659  TDRMLSSSADHIQVGLAANLRDFVLTNHEGKAMKGSEVLTHDGVPVGYALSPTETVNYVS 718

Query: 2107 AHDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDS 2286
            AHDNETLFDIVSLKT +EL VD+RCRMNHLA+S++AL+QGIPFFHSGDE+LRSKSLDRDS
Sbjct: 719  AHDNETLFDIVSLKTVLELCVDDRCRMNHLASSLVALSQGIPFFHSGDELLRSKSLDRDS 778

Query: 2287 YNSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFV 2466
            YNSGDWFNR+DF+Y+SNNWGVGLPPREKNE NWPLIKPRLANPSFKP KHHILAAVDNFV
Sbjct: 779  YNSGDWFNRLDFSYESNNWGVGLPPREKNEKNWPLIKPRLANPSFKPHKHHILAAVDNFV 838

Query: 2467 NLLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYA 2646
            +LLKIRYSSPLFRLRTAN I+ER+RFHNTGP WV G I MS EDGH+G+PG+ Q+DPIY+
Sbjct: 839  DLLKIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGAIVMSVEDGHEGRPGMAQLDPIYS 898

Query: 2647 LLVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSV 2826
             +V++FNACPT+ISL +P LK+R LQLHPVQL   D+LVK+SSY   SG FT+PRRTTSV
Sbjct: 899  YIVIVFNACPTEISLQIPALKSRMLQLHPVQLKLADELVKESSYKAASGCFTIPRRTTSV 958

Query: 2827 FVEVR 2841
            FVEVR
Sbjct: 959  FVEVR 963


>ref|XP_010918920.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Elaeis
            guineensis]
          Length = 946

 Score = 1419 bits (3673), Expect = 0.0
 Identities = 684/905 (75%), Positives = 775/905 (85%), Gaps = 1/905 (0%)
 Frame = +1

Query: 130  IPFCSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIG-DDESSVWLFASRKAA 306
            I  C    A ME TA+ +  ++ + LSYARAYWV+R  IAWNI  DD++ V+L+ASRKA+
Sbjct: 60   ITSCFPVPAAMERTAIAESSLSQETLSYARAYWVSRILIAWNINYDDDAYVYLYASRKAS 119

Query: 307  LFLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVA 486
            + L +E I+GYDVKI L  E  GLP NVT+KFPHI  Y AF++   +D ENLLKCQLA+A
Sbjct: 120  ISLTEEGIEGYDVKILLEPEYYGLPANVTEKFPHIVNYSAFKISESVDFENLLKCQLAIA 179

Query: 487  SFSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPS 666
             F  DGKC NAT LQ+ GVLDD+FAY+GPLGA+FSKE VTLHLWAPTAQEV+VC +++PS
Sbjct: 180  -FYVDGKCKNATGLQLPGVLDDLFAYNGPLGAVFSKEAVTLHLWAPTAQEVSVCIYKEPS 238

Query: 667  SGDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSN 846
             G+ L+ V+LEE  GVWSAVG +SWEG YY+YEV VYHP+T QIE CT  DPYARGLSSN
Sbjct: 239  GGEPLESVQLEEFGGVWSAVGPKSWEGCYYVYEVSVYHPSTSQIEKCTVTDPYARGLSSN 298

Query: 847  GRRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGY 1026
            G+RTWL+NLD ENLKP+GWD LA+EKP+LLS SDISIYELHIRDFSA+D +V+ D +GGY
Sbjct: 299  GKRTWLINLDSENLKPEGWDKLADEKPDLLSFSDISIYELHIRDFSASDHTVNCDIQGGY 358

Query: 1027 LAFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDK 1206
            LAFTSQ+S GICHLKKLSNAGLTH+HLLPSFHFAGVDDDKSNWK  D+ EMAKLP+DSDK
Sbjct: 359  LAFTSQESAGICHLKKLSNAGLTHVHLLPSFHFAGVDDDKSNWKYLDDGEMAKLPADSDK 418

Query: 1207 QQAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLD 1386
            QQAEV  +Q+EDGYNWGYNPV+WGVPKGSYA NPNGP+RILEFRKM+QA+NRIGLRVVLD
Sbjct: 419  QQAEVIGIQNEDGYNWGYNPVLWGVPKGSYAGNPNGPSRILEFRKMIQAVNRIGLRVVLD 478

Query: 1387 VVYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLT 1566
            VVYNHLA+SGP +ENSVLDK+VPGYYLRRNTDG IENS CMNNTASEHFMVERLILDDL 
Sbjct: 479  VVYNHLASSGPLNENSVLDKVVPGYYLRRNTDGAIENSACMNNTASEHFMVERLILDDLL 538

Query: 1567 SWAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVAN 1746
            +WAV YKVDGFRFDLMGHIMK TMVKA+  LQSLSKDK GVDG  IY+YGEGWDFGE+A 
Sbjct: 539  NWAVVYKVDGFRFDLMGHIMKSTMVKARSTLQSLSKDKDGVDGPKIYIYGEGWDFGEIAK 598

Query: 1747 NKRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSV 1926
            NKRGINASQFN+ GTGIGSFNDR+RDAVLGGSPFG+PLQQGF TGLSLEPNGYD GDKSV
Sbjct: 599  NKRGINASQFNISGTGIGSFNDRMRDAVLGGSPFGHPLQQGFVTGLSLEPNGYDHGDKSV 658

Query: 1927 MDFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVS 2106
             D MLS+SADHIQ                   MKGSEV+THDG  VGY LSP E VNYVS
Sbjct: 659  TDRMLSSSADHIQ-------------------MKGSEVLTHDGVPVGYALSPTETVNYVS 699

Query: 2107 AHDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDS 2286
            AHDNETLFDIVSLKT +EL VD+RCRMNHLA+S++AL+QGIPFFHSGDE+LRSKSLDRDS
Sbjct: 700  AHDNETLFDIVSLKTVLELCVDDRCRMNHLASSLVALSQGIPFFHSGDELLRSKSLDRDS 759

Query: 2287 YNSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFV 2466
            YNSGDWFNR+DF+Y+SNNWGVGLPPREKNE NWPLIKPRLANPSFKP KHHILAAVDNFV
Sbjct: 760  YNSGDWFNRLDFSYESNNWGVGLPPREKNEKNWPLIKPRLANPSFKPHKHHILAAVDNFV 819

Query: 2467 NLLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYA 2646
            +LLKIRYSSPLFRLRTAN I+ER+RFHNTGP WV G I MS EDGH+G+PG+ Q+DPIY+
Sbjct: 820  DLLKIRYSSPLFRLRTANAIQERVRFHNTGPSWVPGAIVMSVEDGHEGRPGMAQLDPIYS 879

Query: 2647 LLVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSV 2826
             +V++FNACPT+ISL +P LK+R LQLHPVQL   D+LVK+SSY   SG FT+PRRTTSV
Sbjct: 880  YIVIVFNACPTEISLQIPALKSRMLQLHPVQLKLADELVKESSYKAASGCFTIPRRTTSV 939

Query: 2827 FVEVR 2841
            FVEVR
Sbjct: 940  FVEVR 944


>ref|XP_020084106.1| pullulanase 1, chloroplastic [Ananas comosus]
          Length = 986

 Score = 1411 bits (3653), Expect = 0.0
 Identities = 670/900 (74%), Positives = 784/900 (87%)
 Frame = +1

Query: 148  SEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDED 327
            + A ME T + + P++ D LSY+RAYWV RS IAWN+ +D++S++L+AS+KA +FL D+ 
Sbjct: 87   ASATMENTLLPESPLSQDLLSYSRAYWVARSLIAWNVDEDDNSIYLYASKKANMFLSDDG 146

Query: 328  IQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGK 507
            + GYDVKI L  +   LP NVT+KFPHI  Y+AF++P+  D +NLLK QLAVASF  +GK
Sbjct: 147  VGGYDVKIQLEPDHCALPANVTEKFPHIKDYRAFKVPNS-DVQNLLKSQLAVASFYANGK 205

Query: 508  CTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQR 687
            C NAT LQ+ GVLDD+FAY+GPLGA+FSKE + L+LWAPTAQ+V+ CF+EDPS G  L+ 
Sbjct: 206  CKNATGLQLPGVLDDMFAYNGPLGAVFSKEAIALYLWAPTAQDVSACFYEDPSGGVPLEC 265

Query: 688  VKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLV 867
            ++L+ELNGVWS VG RSW G YY+YE+ VYHP+T +IE C + DPYARGLSS+G+RTWLV
Sbjct: 266  IQLKELNGVWSVVGPRSWAGRYYVYELSVYHPSTSRIEKCESNDPYARGLSSDGKRTWLV 325

Query: 868  NLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQD 1047
            +LD E+LKP GWD LA++KP L S SDISIYELHIRDFSA+D +VD DSRGGY AFTSQD
Sbjct: 326  DLDCEDLKPAGWDELADQKPSLHSFSDISIYELHIRDFSASDHTVDPDSRGGYRAFTSQD 385

Query: 1048 SFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTA 1227
            S G+ HLKKLS+AGLTH+HLLPSFHF GVDD +SNWK  DE  MAKLP DSD+QQA+VTA
Sbjct: 386  SAGVSHLKKLSDAGLTHVHLLPSFHFGGVDDIQSNWKYLDEAAMAKLPPDSDEQQAQVTA 445

Query: 1228 VQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLA 1407
            VQDEDGYNWGYNPV+WGVPKGSYASNPNGP+RILE+R+M+QALNRIGLRVVLDVVYNHL+
Sbjct: 446  VQDEDGYNWGYNPVLWGVPKGSYASNPNGPSRILEYRQMIQALNRIGLRVVLDVVYNHLS 505

Query: 1408 ASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYK 1587
             +GP + NSVLDK+VPGYYLRRN DGGIENSTCMNNTASEHFMVERLILDDL  WAV YK
Sbjct: 506  ENGPFNVNSVLDKVVPGYYLRRNADGGIENSTCMNNTASEHFMVERLILDDLLYWAVAYK 565

Query: 1588 VDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINA 1767
            VDGFRFDLMGHIMK TMVKAK  LQSL+KDK GVDG+ IY+YGEGWDFGEVA N+RGINA
Sbjct: 566  VDGFRFDLMGHIMKSTMVKAKSLLQSLTKDKNGVDGTKIYIYGEGWDFGEVARNQRGINA 625

Query: 1768 SQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLST 1947
            SQ N+ GTGIGSFNDRIRDAVLGGSPFGNPLQQGF TGLSLEPNGYDQGDKSV D MLS+
Sbjct: 626  SQLNIGGTGIGSFNDRIRDAVLGGSPFGNPLQQGFVTGLSLEPNGYDQGDKSVTDRMLSS 685

Query: 1948 SADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETL 2127
            SADHIQVGLA NL+D++LTCH G+ MKGSEV+T+DG  VGY LSP+E VNYVSAHDNETL
Sbjct: 686  SADHIQVGLAGNLRDFVLTCHTGLPMKGSEVLTNDGMPVGYALSPMETVNYVSAHDNETL 745

Query: 2128 FDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWF 2307
            FDIVSLKTP+ELSVD+RCR+NHLA+SIIAL+QG+PFFH+GDE+LRSKSLDRDSYNSGDWF
Sbjct: 746  FDIVSLKTPVELSVDDRCRINHLASSIIALSQGVPFFHAGDEILRSKSLDRDSYNSGDWF 805

Query: 2308 NRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRY 2487
            NR+DF+Y++NNWGVGLP REKNE NWPLIKPRLANPSFKP+K HILA ++NFV+LLKIRY
Sbjct: 806  NRLDFSYETNNWGVGLPLREKNENNWPLIKPRLANPSFKPEKRHILATLNNFVDLLKIRY 865

Query: 2488 SSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFN 2667
            SSPLFRL T N I+ER+RFHNTGP WV G I MS EDGHDGKPGI Q+DPIY+ +VV FN
Sbjct: 866  SSPLFRLETTNSIQERVRFHNTGPSWVCGAIIMSIEDGHDGKPGITQLDPIYSYIVVAFN 925

Query: 2668 ACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVRST 2847
            ACP+++S++VP L++R L+LHPVQL S D LVK+S+Y+  +G FTVP+RTT+VFVEVR T
Sbjct: 926  ACPSEVSIEVPALRSRALELHPVQLKSVDGLVKKSTYEGVAGRFTVPQRTTAVFVEVRGT 985


>ref|XP_020247136.1| LOW QUALITY PROTEIN: pullulanase 1, chloroplastic [Asparagus
            officinalis]
          Length = 960

 Score = 1335 bits (3455), Expect = 0.0
 Identities = 639/904 (70%), Positives = 750/904 (82%)
 Frame = +1

Query: 130  IPFCSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAAL 309
            + FCS    P++E    +  ++ D LS ARAYWVTRS IAWN+GDDE+ VWL+ASR+A L
Sbjct: 55   VSFCSPMLGPVDEPDAAERFLSQDCLSTARAYWVTRSLIAWNVGDDEAVVWLYASRRANL 114

Query: 310  FLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVAS 489
             L DE ++G+D K+ L  E  GLPENV QKFPHI  YKAF +P  +D  +L+KCQLAV S
Sbjct: 115  VLTDEGVEGHDSKVLLEQEPCGLPENVIQKFPHIKHYKAFHVPLSVDVGSLVKCQLAVVS 174

Query: 490  FSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSS 669
            F   GKC NAT LQ+ GVLDD+FAY GPLGA+FS+E +TL+LWAPTAQ V    ++ P  
Sbjct: 175  FCARGKCKNATGLQLPGVLDDMFAYKGPLGAVFSEEAITLNLWAPTAQVVCAHVYKGPLG 234

Query: 670  GDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNG 849
             D L  ++LEE+NGVWSA G RSWEG YY+YEV VYHP+T +IETC   DPYARGLSS+G
Sbjct: 235  EDQLDPMQLEEVNGVWSAAGPRSWEGCYYVYEVSVYHPSTSRIETCKVNDPYARGLSSDG 294

Query: 850  RRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYL 1029
             RT LVNLD    KP GWD+LAN KPE  S +DISIYELH+RDFSA+D +VD + RGG+L
Sbjct: 295  MRTLLVNLDCGKFKPPGWDDLANGKPEFHSFADISIYELHVRDFSASDETVDPNFRGGFL 354

Query: 1030 AFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQ 1209
            AFTSQDS G+ HLKKLS+AGLTH+HLLP F F GVDDD+  W+  D  E++K P DSDKQ
Sbjct: 355  AFTSQDSAGVSHLKKLSDAGLTHVHLLPCFQFGGVDDDRIKWEQLDYDELSKQPPDSDKQ 414

Query: 1210 QAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDV 1389
            Q EVT++QD+DGYNWGYNPV++GVPKGSYASNPNG +RI++FRKM+QALNRIGLRVVLDV
Sbjct: 415  QGEVTSIQDQDGYNWGYNPVLFGVPKGSYASNPNGESRIIDFRKMIQALNRIGLRVVLDV 474

Query: 1390 VYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTS 1569
            VYNHL ASGP D ++V   +VPGYYLRRN DG IENS C+NNTASEHFMVERLI+DDL  
Sbjct: 475  VYNHLYASGPLDLSAVKLXVVPGYYLRRNVDGYIENSACINNTASEHFMVERLIIDDLLC 534

Query: 1570 WAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANN 1749
            WAV YKVDGFRFDLMGHIMK TMVKAK ALQSLSKD+ GVDGS IY+YGEGWDFGEVANN
Sbjct: 535  WAVAYKVDGFRFDLMGHIMKSTMVKAKSALQSLSKDENGVDGSKIYIYGEGWDFGEVANN 594

Query: 1750 KRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVM 1929
             RGINASQFN+ GTGIGSFNDRIRDAVLGGSPFG+PLQQGF TGLSL+PNGYD G K V 
Sbjct: 595  GRGINASQFNIGGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQPNGYDHGSKRVT 654

Query: 1930 DFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSA 2109
            D MLSTS DHIQ+GLAANL+D++LT H G  MKGSE++THDG +VGY L P E +NYVSA
Sbjct: 655  DNMLSTSVDHIQIGLAANLRDFVLTSHTGAAMKGSEILTHDGVAVGYALCPTETINYVSA 714

Query: 2110 HDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSY 2289
            HDNETLFDI+SLKT  E+ VD+RCR+NHLATSI+AL+QG+PFFHSGDE+LRSKSLDRDSY
Sbjct: 715  HDNETLFDIISLKTAEEILVDDRCRINHLATSIVALSQGVPFFHSGDEILRSKSLDRDSY 774

Query: 2290 NSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVN 2469
            NSGDWFNR+DF+Y+ NNWGVGLPPREKNE NWPLIKPRLANPSFKP+KHHILAA+ NF++
Sbjct: 775  NSGDWFNRLDFSYEMNNWGVGLPPREKNEKNWPLIKPRLANPSFKPEKHHILAALGNFID 834

Query: 2470 LLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYAL 2649
            LLKIRYSSPLFRLRTAN+I+ER+ FHNTGP    G I MS EDG++G+PG+ Q+DP Y+ 
Sbjct: 835  LLKIRYSSPLFRLRTANEIQERVCFHNTGPSSCLGAIVMSIEDGYEGEPGMIQLDPNYSF 894

Query: 2650 LVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVF 2829
            +V++FNACPT+ S+ +P L+ R LQLHPVQ+ S D LVK+SSY+ +SG FT+P+RTTSVF
Sbjct: 895  IVIVFNACPTKTSVSLPSLRGRVLQLHPVQVASSDKLVKESSYEASSGCFTIPQRTTSVF 954

Query: 2830 VEVR 2841
            VE R
Sbjct: 955  VEAR 958


>ref|XP_010267258.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nelumbo nucifera]
          Length = 958

 Score = 1322 bits (3421), Expect = 0.0
 Identities = 641/898 (71%), Positives = 749/898 (83%)
 Frame = +1

Query: 148  SEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDED 327
            S  P+E+   I  P     L Y+RAYWVTRS IAWNI   + S +L+ASR A L + ++ 
Sbjct: 63   SSMPLEQ---ISSPQLQCSLLYSRAYWVTRSLIAWNIDIADGSCYLYASRTAGLVVTEDG 119

Query: 328  IQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGK 507
            IQG++VKI L  E SGLP NV +KFPHI   + FR+P+ +D ++L+KCQLAVASF  DG+
Sbjct: 120  IQGHEVKIKLEVENSGLPSNVVEKFPHIRHNRVFRVPTSVDVKSLVKCQLAVASFHSDGQ 179

Query: 508  CTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQR 687
            C+NAT LQ+ GV+DD+FAY GPLGA+F+KE V+L+LWAPTAQ V+   + D S G  L+ 
Sbjct: 180  CSNATGLQLPGVIDDLFAYGGPLGAVFTKEAVSLYLWAPTAQAVSAIIYGDSSGGVPLES 239

Query: 688  VKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLV 867
            V+LEE++GVWSA G R+WEG YY+YEV VYHP+TL+IETC A DPYARGLSS+G+RT LV
Sbjct: 240  VQLEEVDGVWSAHGPRNWEGCYYVYEVSVYHPSTLRIETCMANDPYARGLSSDGKRTLLV 299

Query: 868  NLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQD 1047
            NLD + LKPDGWD LA+EKP+L S SDISIYELHIRDFSA+D +V  D  GGYLAFT QD
Sbjct: 300  NLDCDALKPDGWDALADEKPKLSSFSDISIYELHIRDFSASDQTVHPDFHGGYLAFTLQD 359

Query: 1048 SFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTA 1227
            S G+ HLKKL+ AGLTH+HLLP+F FAGVDD+K  WK  D   +A LP DSD+QQA VT 
Sbjct: 360  SAGVLHLKKLAYAGLTHVHLLPTFQFAGVDDEKK-WKHVDTDMLATLPPDSDQQQARVTE 418

Query: 1228 VQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLA 1407
            +QD+DGYNWGYNPV+WGVPKGSYASNPNG  R +EFRKMVQALNRIGLRV+LDVVYNHL 
Sbjct: 419  IQDDDGYNWGYNPVLWGVPKGSYASNPNGSCRTVEFRKMVQALNRIGLRVILDVVYNHLH 478

Query: 1408 ASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYK 1587
             SGP D+ SVLDK+VPGYYLRRN+DG IENSTC+NNTASEHFMVERLILDDL  WAV+YK
Sbjct: 479  GSGPLDDYSVLDKVVPGYYLRRNSDGCIENSTCINNTASEHFMVERLILDDLLCWAVDYK 538

Query: 1588 VDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINA 1767
            VDGFRFDLMGHIMK TMVKAK ALQSLSK+K GVDGS IY+YGEGWDFGEVA N RGINA
Sbjct: 539  VDGFRFDLMGHIMKSTMVKAKNALQSLSKEKNGVDGSTIYIYGEGWDFGEVAKNGRGINA 598

Query: 1768 SQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLST 1947
            SQFNL G GIGSFNDRIRDAVLGGSPFG+PLQQGF TGLSL+PN +D GDK+ MD ML+ 
Sbjct: 599  SQFNLCGIGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQPNSHDHGDKTSMDHMLAV 658

Query: 1948 SADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETL 2127
            S D+IQVG+AANL+D++LT H GVE+KGSEV+THD   V Y L PIE +NYVSAHDNETL
Sbjct: 659  SQDYIQVGMAANLRDFVLTNHEGVEVKGSEVLTHDQLPVAYALCPIETINYVSAHDNETL 718

Query: 2128 FDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWF 2307
            FDIVS+KTP+E+SVDERCR+NHLATSI+AL+QGIPFFHSGDE+LRSKSLDRDSYNSGDWF
Sbjct: 719  FDIVSMKTPVEISVDERCRINHLATSIVALSQGIPFFHSGDELLRSKSLDRDSYNSGDWF 778

Query: 2308 NRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRY 2487
            NR+DF+Y +NNWGVGLPP+EKNE NWPLIKPRLA+PSFKPQK HILAAV+NF N+L+IRY
Sbjct: 779  NRLDFSYNTNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKRHILAAVENFSNMLRIRY 838

Query: 2488 SSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFN 2667
            SSPLFRLRTAN I+ R+RFHNTGP  V GVI MS EDG++G PG+ Q+DPIY+ +V+IFN
Sbjct: 839  SSPLFRLRTANSIQRRVRFHNTGPSSVPGVIVMSIEDGYEGMPGLAQLDPIYSCIVIIFN 898

Query: 2668 ACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
             CPT++S   P L+ R L+LHPVQL S D +VK S Y+ +SGSF VP RTTSVFVE R
Sbjct: 899  VCPTEVSFGCPALQKRTLKLHPVQLTSTDQIVKNSKYETSSGSFIVPPRTTSVFVESR 956


>ref|XP_023879674.1| pullulanase 1, chloroplastic isoform X2 [Quercus suber]
 gb|POE76574.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 961

 Score = 1321 bits (3420), Expect = 0.0
 Identities = 628/885 (70%), Positives = 747/885 (84%)
 Frame = +1

Query: 187  PVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTE 366
            P + D L ++RAYWV++S IAWN+   + S +LFAS+ AAL ++D+ ++G D++I L  +
Sbjct: 77   PTSQDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKTAALSIVDDGVEGKDMQIKLEED 136

Query: 367  QSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVL 546
              GLPENV +KFPHI  Y+AF++PS LDA+ LLKCQLAVA+F+ DGKC N T LQ+ GVL
Sbjct: 137  SCGLPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLAVATFNSDGKCINVTGLQLPGVL 196

Query: 547  DDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAV 726
            DD+F+Y+GPLGA++S+E+V+L+LWAPTAQ V  C + DP  G+ L+ V+LEE+NGVWS  
Sbjct: 197  DDLFSYNGPLGALYSEESVSLYLWAPTAQAVCACIYRDPVGGNALEIVQLEEVNGVWSTK 256

Query: 727  GSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWD 906
            G + WEG YY+YEV VYHP+TL+I+ C   DPYARGLSS+GRRT+LVNLD + LKP+ WD
Sbjct: 257  GPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLSSDGRRTFLVNLDSDTLKPEEWD 316

Query: 907  NLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNA 1086
            NLANEKP++LS SDISIYELHIRDFSAND +V  + RGGYLAFT QDS G+ HLKKLSNA
Sbjct: 317  NLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRGGYLAFTLQDSSGVLHLKKLSNA 376

Query: 1087 GLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNP 1266
            GLTH+HLLP+F FAGVDD+K NWKC D   + KLP DS +QQ  +TA+QD DGYNWGYNP
Sbjct: 377  GLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDSVQQQDLITAIQDNDGYNWGYNP 436

Query: 1267 VIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDK 1446
            V+WGVPKGSYASNPNGP RI+EFRKM+QALNRIGLR+VLDVVYNH+  SGP DENSVLDK
Sbjct: 437  VLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIVLDVVYNHVHGSGPFDENSVLDK 496

Query: 1447 IVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIM 1626
            IVPGYYLR+NTDG IENSTC+NNTASEH MVERLILDDL  WAV+YKVDGFRFDLMGHIM
Sbjct: 497  IVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDDLLHWAVHYKVDGFRFDLMGHIM 556

Query: 1627 KRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSF 1806
            K TMVKAK AL SL+K++ G+DGS IY+YGEGWDFGEVA N RG+NASQFNL GT IGSF
Sbjct: 557  KSTMVKAKDALHSLTKERDGIDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLSGTQIGSF 616

Query: 1807 NDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANL 1986
            NDRIRDA+LGGSPFG+PLQQGF TGL L+PNG+D G ++  + ML+ + DHIQVG+AANL
Sbjct: 617  NDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNGHDHGTETNAECMLAVAKDHIQVGMAANL 676

Query: 1987 KDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELS 2166
            +D++LT   G E+KGSEV+T+ G  V Y LSP E VNYVSAHDNETLFDIVSLKTP E+S
Sbjct: 677  RDFVLTNFEGKEVKGSEVLTYGGAPVAYALSPTETVNYVSAHDNETLFDIVSLKTPTEIS 736

Query: 2167 VDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWG 2346
            VD+RCR+NHLATS+IAL+QGIPFFHSGDEMLRSKSLDRDSYNSGDWFNR+DFTY SNNWG
Sbjct: 737  VDKRCRINHLATSVIALSQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWG 796

Query: 2347 VGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDI 2526
            VGLPP+EKNE NWPLIKPRLA+PSFKPQK HILAAVDNF+NLL IRYSSPLFRL+TAN I
Sbjct: 797  VGLPPKEKNEKNWPLIKPRLADPSFKPQKIHILAAVDNFLNLLCIRYSSPLFRLKTANAI 856

Query: 2527 KERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPEL 2706
            +ER+RFHNTGP  + G I MS EDG++G PG+ Q+DPIY+ +VVI NA PT++S   P L
Sbjct: 857  QERVRFHNTGPSLIPGTIVMSIEDGYEGVPGLSQLDPIYSYIVVIVNASPTEVSFACPAL 916

Query: 2707 KTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            + R L+LHP+Q+ S D+LVK S+Y+ +SG FTVP RTTSVFVE R
Sbjct: 917  RARTLKLHPIQVMSIDELVKSSTYEESSGCFTVPPRTTSVFVEPR 961


>ref|XP_018808475.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Juglans regia]
          Length = 969

 Score = 1321 bits (3420), Expect = 0.0
 Identities = 627/881 (71%), Positives = 739/881 (83%)
 Frame = +1

Query: 199  DFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQSGL 378
            D L Y+RAYWV+ S IAWN+   + S +LFAS+ A L   D+ ++G DVKI L  +  GL
Sbjct: 87   DSLLYSRAYWVSESTIAWNVDVGDDSCYLFASKSATLSFTDDKVRGEDVKIKLEEDSEGL 146

Query: 379  PENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDDIF 558
            PENVT+KFPHI  Y AF++P  LD + LLKCQLAVA+ + DGKCTNAT LQ+ GVLD++F
Sbjct: 147  PENVTEKFPHIQDYLAFKVPPGLDTKTLLKCQLAVAALNSDGKCTNATGLQLPGVLDELF 206

Query: 559  AYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGSRS 738
            +Y+GPLGA+F +E+V L+LWAPTAQ V  C + DPS GD L+ V+LEE+NGVWS  G + 
Sbjct: 207  SYNGPLGALFFEESVLLYLWAPTAQAVRACIYRDPSGGDPLEIVQLEEVNGVWSTEGPKR 266

Query: 739  WEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNLAN 918
            WEG YY+YEV VYHP TL++E C A DPYARGLSS+G+RT+LVN D + LKP+GWDNLAN
Sbjct: 267  WEGCYYVYEVSVYHPCTLRVEKCHANDPYARGLSSDGKRTFLVNFDSDRLKPEGWDNLAN 326

Query: 919  EKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGLTH 1098
            EKPE+ S SDISIYELHIRDFSAND +V  + RG YLAFT Q S G+ HLKKLS+AG+TH
Sbjct: 327  EKPEIHSFSDISIYELHIRDFSANDHTVPPEFRGCYLAFTLQGSSGVLHLKKLSDAGITH 386

Query: 1099 IHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVIWG 1278
            +HLLP+F FAGVDD+K NWKC D   + +LP DSD+QQA +TA+Q++DGYNWGYNPV+WG
Sbjct: 387  VHLLPTFQFAGVDDEKENWKCVDTKILEQLPPDSDEQQALITAIQNDDGYNWGYNPVLWG 446

Query: 1279 VPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIVPG 1458
            VPKGSYASNPNGP RI+EFRKM+QALNRIGLRVVLDVVYNHL  SGP D+NSVLDKIVPG
Sbjct: 447  VPKGSYASNPNGPCRIIEFRKMIQALNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVPG 506

Query: 1459 YYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKRTM 1638
            YYLRRNTDG IE+STCMNNTASEH MVERLILDDL  WA+++KVDGFRFDLMGHIMK TM
Sbjct: 507  YYLRRNTDGFIEHSTCMNNTASEHVMVERLILDDLLHWAIHHKVDGFRFDLMGHIMKSTM 566

Query: 1639 VKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFNDRI 1818
            VKAK AL SL+K++ G+DGS IY+YGEGWDFGEVA N RG+NASQFNL GTGIGSFNDRI
Sbjct: 567  VKAKDALHSLTKERDGIDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLGGTGIGSFNDRI 626

Query: 1819 RDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKDYI 1998
            RDA+LGGSPFG+PLQQGFATGL L+PNG+DQG ++V + ML+ + DHIQVG+AANL+D++
Sbjct: 627  RDAILGGSPFGHPLQQGFATGLLLQPNGHDQGTEAVSECMLAVAKDHIQVGMAANLRDFV 686

Query: 1999 LTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVDER 2178
            +T + G E+KGSEV+THDG  V Y L   E VNYVSAHDNETLFDIVSLKTP E+SVDER
Sbjct: 687  ITSYQGEEVKGSEVLTHDGTPVAYALCSTETVNYVSAHDNETLFDIVSLKTPKEISVDER 746

Query: 2179 CRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVGLP 2358
            CR+NHLAT +IAL+QGIPFFHSGDE+LRSKSLDRDSYNSGDWFNR+DFTY SNNWGVGLP
Sbjct: 747  CRINHLATGVIALSQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGLP 806

Query: 2359 PREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKERL 2538
            P+EKNE NWPLIKPRLA+PSFKPQ+ HILAAVDNF+NLL+IRYSSPLFRLRTAN I+ER+
Sbjct: 807  PKEKNEKNWPLIKPRLADPSFKPQRVHILAAVDNFLNLLRIRYSSPLFRLRTANAIQERV 866

Query: 2539 RFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKTRK 2718
             FHNTGP    GVI MS +DGH+G PG+ Q+DP Y+ +VVI NACPT++S   P L+TR 
Sbjct: 867  HFHNTGPSGAPGVIVMSIQDGHEGVPGLSQLDPTYSYIVVIINACPTKVSFACPALRTRT 926

Query: 2719 LQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            L+LHPVQ  S D+LVK S+Y+ +SG   VP RTT VFVE R
Sbjct: 927  LKLHPVQAMSTDELVKSSTYEASSGCLAVPPRTTVVFVEPR 967


>ref|XP_010658333.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Vitis vinifera]
          Length = 954

 Score = 1321 bits (3420), Expect = 0.0
 Identities = 638/904 (70%), Positives = 743/904 (82%)
 Frame = +1

Query: 130  IPFCSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAAL 309
            I FCS    P+E    +    A D L Y+RAYWV+ S IAWN+   + S +L++S+ AAL
Sbjct: 53   ISFCSCLPMPLE----VSTSTAQDSLLYSRAYWVSESIIAWNVDVGDGSCYLYSSKIAAL 108

Query: 310  FLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVAS 489
             + D  I G+D+ I L  +  GLP NV +KFP +  YKAF++P  +DA++L+KCQLAVA+
Sbjct: 109  SIGDSGITGHDMTIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLAVAA 168

Query: 490  FSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSS 669
            FS DG+C N T LQ+ GVLD++F+Y GPLGAIFSKE VTL+LWAPTAQ V+ C + DPS 
Sbjct: 169  FSCDGQCNNVTGLQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGDPSG 228

Query: 670  GDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNG 849
            GD L+ V LEE NGVWSA G +SWEG YY YEV VYHP+TLQIE CTA DPYARGLS++ 
Sbjct: 229  GDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLSADA 288

Query: 850  RRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYL 1029
            RRT LVNLD ++LKP+GWDNLA+EKP LLS SDISIYELHIRDFSA+D +V  D RGGYL
Sbjct: 289  RRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDFRGGYL 348

Query: 1030 AFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQ 1209
            AFTSQDS GI HLKKL NAG++H+HLLP+F FAGVDD+K  WKC D   +  LP DS +Q
Sbjct: 349  AFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDSVEQ 408

Query: 1210 QAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDV 1389
            QA++  +QDEDGYNWGYNPV+WGVPKGSYASNPN P R LEFRKMVQALNRIG RVVLDV
Sbjct: 409  QAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDV 468

Query: 1390 VYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTS 1569
            VYNHL  SGP DENSVLDKIVPGYYLRRN+DG IE+S C+NNTASEHFMVERLILDDL  
Sbjct: 469  VYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFMVERLILDDLLC 528

Query: 1570 WAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANN 1749
            WAV+YKVDGFRFDLMGHIMKRTMVKAK  L SLSKD  G+DGS IY+YGEGWDFGEVA N
Sbjct: 529  WAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYGEGWDFGEVAKN 588

Query: 1750 KRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVM 1929
             RG+NASQFNL GTGIGSFNDRIRDA+LGGSPFG+PLQQGF TGL L+PN +D G + V 
Sbjct: 589  GRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNDHDHGGEEVA 648

Query: 1930 DFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSA 2109
            + ML+ S DHIQVG+AANLKD++LT   G E+KGSEV T+DG  V YT+ P E +NYVSA
Sbjct: 649  ERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTVCPTETINYVSA 708

Query: 2110 HDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSY 2289
            HDNETLFDIVSLKTPM++SV+ERCR+NHLATSIIAL+QGIPFFHSGDEMLRSKS+DRDSY
Sbjct: 709  HDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSGDEMLRSKSIDRDSY 768

Query: 2290 NSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVN 2469
            NSGDWFNR+DF+Y SNNWGVGLPP+EKNE NWPLIKPRLA+PSFKPQK+HI+AAV+NF+N
Sbjct: 769  NSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKNHIIAAVENFLN 828

Query: 2470 LLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYAL 2649
            LLKIRYSSPLFRLRTAN I+ R+RFHNTGP WV G+I MS EDGH+G PG+ Q+DP+Y+ 
Sbjct: 829  LLKIRYSSPLFRLRTANSIQARVRFHNTGPSWVPGIIVMSIEDGHEGVPGLSQLDPVYSY 888

Query: 2650 LVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVF 2829
            +VVI N  PT+I    P L+ R LQLHPVQ+ S D ++K S+Y+ +SG F VP RTTSVF
Sbjct: 889  IVVIINPSPTEIKFASPALQARTLQLHPVQVMSSDGVIKNSTYEASSGCFIVPPRTTSVF 948

Query: 2830 VEVR 2841
            VE R
Sbjct: 949  VEPR 952


>ref|XP_002271820.3| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Vitis vinifera]
 emb|CBI31395.3| unnamed protein product, partial [Vitis vinifera]
          Length = 956

 Score = 1320 bits (3415), Expect = 0.0
 Identities = 640/907 (70%), Positives = 745/907 (82%), Gaps = 3/907 (0%)
 Frame = +1

Query: 130  IPFCSLSEAPME---ETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRK 300
            I FCS    P+E    TA +Q     D L Y+RAYWV+ S IAWN+   + S +L++S+ 
Sbjct: 53   ISFCSCLPMPLEVSTSTAQLQ-----DSLLYSRAYWVSESIIAWNVDVGDGSCYLYSSKI 107

Query: 301  AALFLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLA 480
            AAL + D  I G+D+ I L  +  GLP NV +KFP +  YKAF++P  +DA++L+KCQLA
Sbjct: 108  AALSIGDSGITGHDMTIQLEEDNGGLPINVIEKFPQLQGYKAFKVPQAVDAKSLIKCQLA 167

Query: 481  VASFSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFED 660
            VA+FS DG+C N T LQ+ GVLD++F+Y GPLGAIFSKE VTL+LWAPTAQ V+ C + D
Sbjct: 168  VAAFSCDGQCNNVTGLQLPGVLDELFSYEGPLGAIFSKEAVTLYLWAPTAQVVHACIYGD 227

Query: 661  PSSGDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLS 840
            PS GD L+ V LEE NGVWSA G +SWEG YY YEV VYHP+TLQIE CTA DPYARGLS
Sbjct: 228  PSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVYHPSTLQIEKCTANDPYARGLS 287

Query: 841  SNGRRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRG 1020
            ++ RRT LVNLD ++LKP+GWDNLA+EKP LLS SDISIYELHIRDFSA+D +V  D RG
Sbjct: 288  ADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSASDHTVHPDFRG 347

Query: 1021 GYLAFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDS 1200
            GYLAFTSQDS GI HLKKL NAG++H+HLLP+F FAGVDD+K  WKC D   +  LP DS
Sbjct: 348  GYLAFTSQDSAGILHLKKLCNAGISHLHLLPTFQFAGVDDEKDKWKCVDPNILETLPPDS 407

Query: 1201 DKQQAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVV 1380
             +QQA++  +QDEDGYNWGYNPV+WGVPKGSYASNPN P R LEFRKMVQALNRIG RVV
Sbjct: 408  VEQQAQIMTIQDEDGYNWGYNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVV 467

Query: 1381 LDVVYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDD 1560
            LDVVYNHL  SGP DENSVLDKIVPGYYLRRN+DG IE+S C+NNTASEHFMVERLILDD
Sbjct: 468  LDVVYNHLHGSGPFDENSVLDKIVPGYYLRRNSDGCIEHSACVNNTASEHFMVERLILDD 527

Query: 1561 LTSWAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEV 1740
            L  WAV+YKVDGFRFDLMGHIMKRTMVKAK  L SLSKD  G+DGS IY+YGEGWDFGEV
Sbjct: 528  LLCWAVDYKVDGFRFDLMGHIMKRTMVKAKNMLHSLSKDTDGIDGSSIYIYGEGWDFGEV 587

Query: 1741 ANNKRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDK 1920
            A N RG+NASQFNL GTGIGSFNDRIRDA+LGGSPFG+PLQQGF TGL L+PN +D G +
Sbjct: 588  AKNGRGVNASQFNLCGTGIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNDHDHGGE 647

Query: 1921 SVMDFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNY 2100
             V + ML+ S DHIQVG+AANLKD++LT   G E+KGSEV T+DG  V YT+ P E +NY
Sbjct: 648  EVAERMLAVSKDHIQVGMAANLKDFVLTNCEGEEVKGSEVSTYDGTPVAYTVCPTETINY 707

Query: 2101 VSAHDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDR 2280
            VSAHDNETLFDIVSLKTPM++SV+ERCR+NHLATSIIAL+QGIPFFHSGDEMLRSKS+DR
Sbjct: 708  VSAHDNETLFDIVSLKTPMQISVEERCRINHLATSIIALSQGIPFFHSGDEMLRSKSIDR 767

Query: 2281 DSYNSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDN 2460
            DSYNSGDWFNR+DF+Y SNNWGVGLPP+EKNE NWPLIKPRLA+PSFKPQK+HI+AAV+N
Sbjct: 768  DSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKNHIIAAVEN 827

Query: 2461 FVNLLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPI 2640
            F+NLLKIRYSSPLFRLRTAN I+ R+RFHNTGP WV G+I MS EDGH+G PG+ Q+DP+
Sbjct: 828  FLNLLKIRYSSPLFRLRTANSIQARVRFHNTGPSWVPGIIVMSIEDGHEGVPGLSQLDPV 887

Query: 2641 YALLVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTT 2820
            Y+ +VVI N  PT+I    P L+ R LQLHPVQ+ S D ++K S+Y+ +SG F VP RTT
Sbjct: 888  YSYIVVIINPSPTEIKFASPALQARTLQLHPVQVMSSDGVIKNSTYEASSGCFIVPPRTT 947

Query: 2821 SVFVEVR 2841
            SVFVE R
Sbjct: 948  SVFVEPR 954


>ref|XP_023879673.1| pullulanase 1, chloroplastic isoform X1 [Quercus suber]
 gb|POE76573.1| pullulanase 1, chloroplastic [Quercus suber]
          Length = 963

 Score = 1319 bits (3414), Expect = 0.0
 Identities = 632/901 (70%), Positives = 751/901 (83%)
 Frame = +1

Query: 139  CSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLL 318
            CS S +    T   Q     D L ++RAYWV++S IAWN+   + S +LFAS+ AAL ++
Sbjct: 66   CSSSMSVENSTPTSQ---LQDSLLFSRAYWVSKSTIAWNVDVGDGSCFLFASKTAALSIV 122

Query: 319  DEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSG 498
            D+ ++G D++I L  +  GLPENV +KFPHI  Y+AF++PS LDA+ LLKCQLAVA+F+ 
Sbjct: 123  DDGVEGKDMQIKLEEDSCGLPENVIRKFPHIQDYRAFKVPSTLDAKTLLKCQLAVATFNS 182

Query: 499  DGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDL 678
            DGKC N T LQ+ GVLDD+F+Y+GPLGA++S+E+V+L+LWAPTAQ V  C + DP  G+ 
Sbjct: 183  DGKCINVTGLQLPGVLDDLFSYNGPLGALYSEESVSLYLWAPTAQAVCACIYRDPVGGNA 242

Query: 679  LQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRT 858
            L+ V+LEE+NGVWS  G + WEG YY+YEV VYHP+TL+I+ C   DPYARGLSS+GRRT
Sbjct: 243  LEIVQLEEVNGVWSTKGPKRWEGCYYVYEVSVYHPSTLRIDKCHVNDPYARGLSSDGRRT 302

Query: 859  WLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFT 1038
            +LVNLD + LKP+ WDNLANEKP++LS SDISIYELHIRDFSAND +V  + RGGYLAFT
Sbjct: 303  FLVNLDSDTLKPEEWDNLANEKPDILSFSDISIYELHIRDFSANDYTVHPEFRGGYLAFT 362

Query: 1039 SQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAE 1218
             QDS G+ HLKKLSNAGLTH+HLLP+F FAGVDD+K NWKC D   + KLP DS +QQ  
Sbjct: 363  LQDSSGVLHLKKLSNAGLTHVHLLPTFQFAGVDDEKENWKCVDTNMLEKLPPDSVQQQDL 422

Query: 1219 VTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYN 1398
            +TA+QD DGYNWGYNPV+WGVPKGSYASNPNGP RI+EFRKM+QALNRIGLR+VLDVVYN
Sbjct: 423  ITAIQDNDGYNWGYNPVLWGVPKGSYASNPNGPYRIIEFRKMIQALNRIGLRIVLDVVYN 482

Query: 1399 HLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAV 1578
            H+  SGP DENSVLDKIVPGYYLR+NTDG IENSTC+NNTASEH MVERLILDDL  WAV
Sbjct: 483  HVHGSGPFDENSVLDKIVPGYYLRKNTDGLIENSTCVNNTASEHVMVERLILDDLLHWAV 542

Query: 1579 NYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRG 1758
            +YKVDGFRFDLMGHIMK TMVKAK AL SL+K++ G+DGS IY+YGEGWDFGEVA N RG
Sbjct: 543  HYKVDGFRFDLMGHIMKSTMVKAKDALHSLTKERDGIDGSSIYIYGEGWDFGEVAKNGRG 602

Query: 1759 INASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFM 1938
            +NASQFNL GT IGSFNDRIRDA+LGGSPFG+PLQQGF TGL L+PNG+D G ++  + M
Sbjct: 603  VNASQFNLSGTQIGSFNDRIRDAMLGGSPFGHPLQQGFVTGLFLQPNGHDHGTETNAECM 662

Query: 1939 LSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDN 2118
            L+ + DHIQVG+AANL+D++LT   G E+KGSEV+T+ G  V Y LSP E VNYVSAHDN
Sbjct: 663  LAVAKDHIQVGMAANLRDFVLTNFEGKEVKGSEVLTYGGAPVAYALSPTETVNYVSAHDN 722

Query: 2119 ETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSG 2298
            ETLFDIVSLKTP E+SVD+RCR+NHLATS+IAL+QGIPFFHSGDEMLRSKSLDRDSYNSG
Sbjct: 723  ETLFDIVSLKTPTEISVDKRCRINHLATSVIALSQGIPFFHSGDEMLRSKSLDRDSYNSG 782

Query: 2299 DWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLK 2478
            DWFNR+DFTY SNNWGVGLPP+EKNE NWPLIKPRLA+PSFKPQK HILAAVDNF+NLL 
Sbjct: 783  DWFNRLDFTYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSFKPQKIHILAAVDNFLNLLC 842

Query: 2479 IRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVV 2658
            IRYSSPLFRL+TAN I+ER+RFHNTGP  + G I MS EDG++G PG+ Q+DPIY+ +VV
Sbjct: 843  IRYSSPLFRLKTANAIQERVRFHNTGPSLIPGTIVMSIEDGYEGVPGLSQLDPIYSYIVV 902

Query: 2659 IFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEV 2838
            I NA PT++S   P L+ R L+LHP+Q+ S D+LVK S+Y+ +SG FTVP RTTSVFVE 
Sbjct: 903  IVNASPTEVSFACPALRARTLKLHPIQVMSIDELVKSSTYEESSGCFTVPPRTTSVFVEP 962

Query: 2839 R 2841
            R
Sbjct: 963  R 963


>gb|OVA17964.1| Glycoside hydrolase [Macleaya cordata]
          Length = 983

 Score = 1319 bits (3414), Expect = 0.0
 Identities = 627/879 (71%), Positives = 740/879 (84%)
 Frame = +1

Query: 205  LSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQSGLPE 384
            LSY+RAYWV  S IAWN+   + S +L+AS+ A L L ++ I+GYDVKI L  + SGLP 
Sbjct: 103  LSYSRAYWVNGSLIAWNVDIGDGSCYLYASKTANLVLTNDGIEGYDVKIKLEPDHSGLPV 162

Query: 385  NVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDDIFAY 564
            NV +KFPHI  Y  F++P  LDA+NLLKCQLAV +F  DG+C++AT LQ+ GVLDD+F+Y
Sbjct: 163  NVIEKFPHIQNYNVFKVPFGLDAKNLLKCQLAVVAFKADGQCSDATGLQLPGVLDDLFSY 222

Query: 565  SGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGSRSWE 744
             GPLGA+FSKE V+L++WAPTAQ V  C + +PS G  ++ ++LEE+NGVWSA GSRSWE
Sbjct: 223  VGPLGAVFSKEAVSLYIWAPTAQVVRACIYREPSGGSPIEIIRLEEVNGVWSAHGSRSWE 282

Query: 745  GLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNLANEK 924
            G YY+YEV VYHP+T  +ETC A DPYARGLSS+G+RT LVNLD ++LKP+GW+ L ++K
Sbjct: 283  GCYYVYEVSVYHPSTSLVETCIANDPYARGLSSDGKRTLLVNLDCDSLKPEGWEKLVDQK 342

Query: 925  PELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGLTHIH 1104
            PE  S SD+SIYELHIRDFSAND +V+ D +GGYLAFTSQDS GI HLKKL NAGLTH+H
Sbjct: 343  PEFSSFSDVSIYELHIRDFSANDHTVNPDFQGGYLAFTSQDSAGIRHLKKLHNAGLTHLH 402

Query: 1105 LLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVIWGVP 1284
            LLP+F FA V D+K  WK  D  ++A LP DSD+QQA+VTA+QDEDGYNWGYNPV+WGVP
Sbjct: 403  LLPTFQFADVADEKEKWKYLDTDKLATLPPDSDEQQAQVTAIQDEDGYNWGYNPVLWGVP 462

Query: 1285 KGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIVPGYY 1464
            KGSYASNPNG  R LEFRKMVQ+LNRIGLRVVLDVVYNHL  +GP  +NSVLDK+VPGYY
Sbjct: 463  KGSYASNPNGSCRTLEFRKMVQSLNRIGLRVVLDVVYNHLHGNGPHGDNSVLDKVVPGYY 522

Query: 1465 LRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKRTMVK 1644
            LRRN+DG IE+STC+NNTASEHFM ERLILDD+  WAV+YKVDGFRFDLMGHIMKRTM+K
Sbjct: 523  LRRNSDGCIEHSTCVNNTASEHFMAERLILDDILCWAVDYKVDGFRFDLMGHIMKRTMLK 582

Query: 1645 AKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFNDRIRD 1824
            A  AL SLSKD+ GVDG  IY+YGEGWDFGEVA N RG+NASQFNL GTGIGSFNDR+RD
Sbjct: 583  AYSALHSLSKDQNGVDGPKIYIYGEGWDFGEVAKNGRGVNASQFNLCGTGIGSFNDRMRD 642

Query: 1825 AVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKDYILT 2004
            AVLGGSPFG+PLQQGF TGLS +PNG+D G K V+D ML+ S DHIQV +A NLKD++LT
Sbjct: 643  AVLGGSPFGHPLQQGFVTGLSFQPNGHDHGVKIVVDSMLAASQDHIQVAMAGNLKDFVLT 702

Query: 2005 CHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVDERCR 2184
             H G E+KGSEV+THDG  V Y L PIE VNYVSAHDNETLFDI+SLKTPME+SVDERCR
Sbjct: 703  SHLGEEVKGSEVLTHDGLPVAYALCPIETVNYVSAHDNETLFDIISLKTPMEISVDERCR 762

Query: 2185 MNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVGLPPR 2364
            +NHLATSIIAL+QG+PFFH GDEMLRSKSLDRDSYNSGDWFNR+DF+Y++NNWGVGLPP+
Sbjct: 763  LNHLATSIIALSQGVPFFHCGDEMLRSKSLDRDSYNSGDWFNRLDFSYETNNWGVGLPPK 822

Query: 2365 EKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKERLRF 2544
             KNE NW LIKPRLA+PSFKPQK+HIL AV+NF+NLL+IRYSSPLFRLRTAN ++ER+RF
Sbjct: 823  GKNEKNWSLIKPRLADPSFKPQKNHILTAVENFLNLLRIRYSSPLFRLRTANAVQERVRF 882

Query: 2545 HNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKTRKLQ 2724
            HNTGP  V G+I MS EDGH+GKPG+ Q+DP Y+ +VV FNACP +++   P L+ R LQ
Sbjct: 883  HNTGPSLVPGIIVMSIEDGHEGKPGLAQLDPTYSYIVVAFNACPGEVTFTSPALRARTLQ 942

Query: 2725 LHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            LHPVQL S D++VK S+YD +SGSFTVP RTT+VFVE R
Sbjct: 943  LHPVQLMSSDEVVKNSNYDASSGSFTVPPRTTAVFVESR 981


>gb|ONK57269.1| uncharacterized protein A4U43_C10F18340 [Asparagus officinalis]
          Length = 947

 Score = 1318 bits (3410), Expect = 0.0
 Identities = 633/904 (70%), Positives = 742/904 (82%)
 Frame = +1

Query: 130  IPFCSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAAL 309
            + FCS    P++E    +  ++ D LS ARAYWVTRS IAWN+GDDE+ VWL+ASR+A L
Sbjct: 55   VSFCSPMLGPVDEPDAAERFLSQDCLSTARAYWVTRSLIAWNVGDDEAVVWLYASRRANL 114

Query: 310  FLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVAS 489
             L DE ++G+D K+ L  E  GLPENV QKFPHI  YKAF +P  +D  +L+KCQLAV S
Sbjct: 115  VLTDEGVEGHDSKVLLEQEPCGLPENVIQKFPHIKHYKAFHVPLSVDVGSLVKCQLAVVS 174

Query: 490  FSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSS 669
            F   GKC NAT LQ+ GVLDD+FAY GPLGA+FS+E +TL+LWAPTAQ V    ++ P  
Sbjct: 175  FCARGKCKNATGLQLPGVLDDMFAYKGPLGAVFSEEAITLNLWAPTAQVVCAHVYKGPLG 234

Query: 670  GDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNG 849
             D L  ++LEE+NGVWSA G RSWEG YY+YEV VYHP+T +IETC   DPYARGLSS+G
Sbjct: 235  EDQLDPMQLEEVNGVWSAAGPRSWEGCYYVYEVSVYHPSTSRIETCKVNDPYARGLSSDG 294

Query: 850  RRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYL 1029
             RT LVNLD    KP GWD+LAN KPE  S +DISIYELH+RDFSA+D +VD + RGG+L
Sbjct: 295  MRTLLVNLDCGKFKPPGWDDLANGKPEFHSFADISIYELHVRDFSASDETVDPNFRGGFL 354

Query: 1030 AFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQ 1209
            AFTSQDS G+ HLKKLS+AGLTH+HLLP F F GVDDD+  W+  D  E++K P DSDKQ
Sbjct: 355  AFTSQDSAGVSHLKKLSDAGLTHVHLLPCFQFGGVDDDRIKWEQLDYDELSKQPPDSDKQ 414

Query: 1210 QAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDV 1389
            Q EVT++QD+DGYNWGYNPV++GVPKGSYASNPNG +RI++FRKM+QALNRIGLRVVLDV
Sbjct: 415  QGEVTSIQDQDGYNWGYNPVLFGVPKGSYASNPNGESRIIDFRKMIQALNRIGLRVVLDV 474

Query: 1390 VYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTS 1569
            VYNHL              +VPGYYLRRN DG IENS C+NNTASEHFMVERLI+DDL  
Sbjct: 475  VYNHLY-------------VVPGYYLRRNVDGYIENSACINNTASEHFMVERLIIDDLLC 521

Query: 1570 WAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANN 1749
            WAV YKVDGFRFDLMGHIMK TMVKAK ALQSLSKD+ GVDGS IY+YGEGWDFGEVANN
Sbjct: 522  WAVAYKVDGFRFDLMGHIMKSTMVKAKSALQSLSKDENGVDGSKIYIYGEGWDFGEVANN 581

Query: 1750 KRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVM 1929
             RGINASQFN+ GTGIGSFNDRIRDAVLGGSPFG+PLQQGF TGLSL+PNGYD G K V 
Sbjct: 582  GRGINASQFNIGGTGIGSFNDRIRDAVLGGSPFGHPLQQGFVTGLSLQPNGYDHGSKRVT 641

Query: 1930 DFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSA 2109
            D MLSTS DHIQ+GLAANL+D++LT H G  MKGSE++THDG +VGY L P E +NYVSA
Sbjct: 642  DNMLSTSVDHIQIGLAANLRDFVLTSHTGAAMKGSEILTHDGVAVGYALCPTETINYVSA 701

Query: 2110 HDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSY 2289
            HDNETLFDI+SLKT  E+ VD+RCR+NHLATSI+AL+QG+PFFHSGDE+LRSKSLDRDSY
Sbjct: 702  HDNETLFDIISLKTAEEILVDDRCRINHLATSIVALSQGVPFFHSGDEILRSKSLDRDSY 761

Query: 2290 NSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVN 2469
            NSGDWFNR+DF+Y+ NNWGVGLPPREKNE NWPLIKPRLANPSFKP+KHHILAA+ NF++
Sbjct: 762  NSGDWFNRLDFSYEMNNWGVGLPPREKNEKNWPLIKPRLANPSFKPEKHHILAALGNFID 821

Query: 2470 LLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYAL 2649
            LLKIRYSSPLFRLRTAN+I+ER+ FHNTGP    G I MS EDG++G+PG+ Q+DP Y+ 
Sbjct: 822  LLKIRYSSPLFRLRTANEIQERVCFHNTGPSSCLGAIVMSIEDGYEGEPGMIQLDPNYSF 881

Query: 2650 LVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVF 2829
            +V++FNACPT+ S+ +P L+ R LQLHPVQ+ S D LVK+SSY+ +SG FT+P+RTTSVF
Sbjct: 882  IVIVFNACPTKTSVSLPSLRGRVLQLHPVQVASSDKLVKESSYEASSGCFTIPQRTTSVF 941

Query: 2830 VEVR 2841
            VE R
Sbjct: 942  VEAR 945


>ref|XP_018808474.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Juglans regia]
          Length = 970

 Score = 1317 bits (3408), Expect = 0.0
 Identities = 627/882 (71%), Positives = 739/882 (83%), Gaps = 1/882 (0%)
 Frame = +1

Query: 199  DFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQSGL 378
            D L Y+RAYWV+ S IAWN+   + S +LFAS+ A L   D+ ++G DVKI L  +  GL
Sbjct: 87   DSLLYSRAYWVSESTIAWNVDVGDDSCYLFASKSATLSFTDDKVRGEDVKIKLEEDSEGL 146

Query: 379  PENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDDIF 558
            PENVT+KFPHI  Y AF++P  LD + LLKCQLAVA+ + DGKCTNAT LQ+ GVLD++F
Sbjct: 147  PENVTEKFPHIQDYLAFKVPPGLDTKTLLKCQLAVAALNSDGKCTNATGLQLPGVLDELF 206

Query: 559  AYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGSRS 738
            +Y+GPLGA+F +E+V L+LWAPTAQ V  C + DPS GD L+ V+LEE+NGVWS  G + 
Sbjct: 207  SYNGPLGALFFEESVLLYLWAPTAQAVRACIYRDPSGGDPLEIVQLEEVNGVWSTEGPKR 266

Query: 739  WEGLYYLYEVMVYHPTTLQIETCTAVDPYARG-LSSNGRRTWLVNLDDENLKPDGWDNLA 915
            WEG YY+YEV VYHP TL++E C A DPYARG LSS+G+RT+LVN D + LKP+GWDNLA
Sbjct: 267  WEGCYYVYEVSVYHPCTLRVEKCHANDPYARGRLSSDGKRTFLVNFDSDRLKPEGWDNLA 326

Query: 916  NEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGLT 1095
            NEKPE+ S SDISIYELHIRDFSAND +V  + RG YLAFT Q S G+ HLKKLS+AG+T
Sbjct: 327  NEKPEIHSFSDISIYELHIRDFSANDHTVPPEFRGCYLAFTLQGSSGVLHLKKLSDAGIT 386

Query: 1096 HIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVIW 1275
            H+HLLP+F FAGVDD+K NWKC D   + +LP DSD+QQA +TA+Q++DGYNWGYNPV+W
Sbjct: 387  HVHLLPTFQFAGVDDEKENWKCVDTKILEQLPPDSDEQQALITAIQNDDGYNWGYNPVLW 446

Query: 1276 GVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIVP 1455
            GVPKGSYASNPNGP RI+EFRKM+QALNRIGLRVVLDVVYNHL  SGP D+NSVLDKIVP
Sbjct: 447  GVPKGSYASNPNGPCRIIEFRKMIQALNRIGLRVVLDVVYNHLQGSGPFDDNSVLDKIVP 506

Query: 1456 GYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKRT 1635
            GYYLRRNTDG IE+STCMNNTASEH MVERLILDDL  WA+++KVDGFRFDLMGHIMK T
Sbjct: 507  GYYLRRNTDGFIEHSTCMNNTASEHVMVERLILDDLLHWAIHHKVDGFRFDLMGHIMKST 566

Query: 1636 MVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFNDR 1815
            MVKAK AL SL+K++ G+DGS IY+YGEGWDFGEVA N RG+NASQFNL GTGIGSFNDR
Sbjct: 567  MVKAKDALHSLTKERDGIDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLGGTGIGSFNDR 626

Query: 1816 IRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKDY 1995
            IRDA+LGGSPFG+PLQQGFATGL L+PNG+DQG ++V + ML+ + DHIQVG+AANL+D+
Sbjct: 627  IRDAILGGSPFGHPLQQGFATGLLLQPNGHDQGTEAVSECMLAVAKDHIQVGMAANLRDF 686

Query: 1996 ILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVDE 2175
            ++T + G E+KGSEV+THDG  V Y L   E VNYVSAHDNETLFDIVSLKTP E+SVDE
Sbjct: 687  VITSYQGEEVKGSEVLTHDGTPVAYALCSTETVNYVSAHDNETLFDIVSLKTPKEISVDE 746

Query: 2176 RCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVGL 2355
            RCR+NHLAT +IAL+QGIPFFHSGDE+LRSKSLDRDSYNSGDWFNR+DFTY SNNWGVGL
Sbjct: 747  RCRINHLATGVIALSQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGL 806

Query: 2356 PPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKER 2535
            PP+EKNE NWPLIKPRLA+PSFKPQ+ HILAAVDNF+NLL+IRYSSPLFRLRTAN I+ER
Sbjct: 807  PPKEKNEKNWPLIKPRLADPSFKPQRVHILAAVDNFLNLLRIRYSSPLFRLRTANAIQER 866

Query: 2536 LRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKTR 2715
            + FHNTGP    GVI MS +DGH+G PG+ Q+DP Y+ +VVI NACPT++S   P L+TR
Sbjct: 867  VHFHNTGPSGAPGVIVMSIQDGHEGVPGLSQLDPTYSYIVVIINACPTKVSFACPALRTR 926

Query: 2716 KLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
             L+LHPVQ  S D+LVK S+Y+ +SG   VP RTT VFVE R
Sbjct: 927  TLKLHPVQAMSTDELVKSSTYEASSGCLAVPPRTTVVFVEPR 968


>ref|XP_007220271.1| pullulanase 1, chloroplastic [Prunus persica]
 gb|ONI23747.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 965

 Score = 1316 bits (3407), Expect = 0.0
 Identities = 629/879 (71%), Positives = 745/879 (84%)
 Frame = +1

Query: 205  LSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQSGLPE 384
            L Y+RA+WV+ S IAWN+     S +L AS+ AAL L  + I G DVK+ L  ++ GLPE
Sbjct: 85   LLYSRAFWVSESIIAWNVYVGNGSCYLLASKTAALSLTSDGILGEDVKVKLEEDKHGLPE 144

Query: 385  NVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDDIFAY 564
            NV +KFPHI  Y+AF +P  LDA+ LLKCQLAVA+F+ DG+C++AT LQ+ G+LDD+F+Y
Sbjct: 145  NVKEKFPHIKDYRAFNVPPDLDAKPLLKCQLAVATFNSDGRCSDATGLQLPGILDDLFSY 204

Query: 565  SGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGSRSWE 744
            +GPLGA++SK++V+L+LWAPTAQEV VC +++PS G  L+ V+LEE NGVWS  G +SWE
Sbjct: 205  NGPLGALYSKQSVSLYLWAPTAQEVCVCIYKEPSGGSPLEVVQLEEFNGVWSTKGPKSWE 264

Query: 745  GLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNLANEK 924
            G YY+YEV VYHP+TL+IE C A DPYARGLSS+GRRT LVNLD +N+KP+GWD L +EK
Sbjct: 265  GCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRRTLLVNLDSDNIKPEGWDKLVDEK 324

Query: 925  PELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGLTHIH 1104
            P++LS SDISIYELHIRDFSA+D +V  + RGGYLAFT QDS G  HLKKLSNAG+TH+H
Sbjct: 325  PDILSFSDISIYELHIRDFSASDQAVHPEFRGGYLAFTLQDSAGAIHLKKLSNAGITHVH 384

Query: 1105 LLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVIWGVP 1284
            LLP+F FAGVDD+K NWK  D   + K PSDSDKQQA +TA+Q++DGYNWGYNPV+WGVP
Sbjct: 385  LLPAFQFAGVDDEKENWKSVDPEVLEKFPSDSDKQQALITAIQNDDGYNWGYNPVLWGVP 444

Query: 1285 KGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIVPGYY 1464
            KGSYASN NG  R +EFRKMVQALNR GLRVVLDVVYNHL  SGP D+NSVLDKIVPGYY
Sbjct: 445  KGSYASNANGTYRAIEFRKMVQALNRFGLRVVLDVVYNHLHGSGPVDDNSVLDKIVPGYY 504

Query: 1465 LRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKRTMVK 1644
            LRRNTDG IE+STC+NNTASEHFMVERLI+DDL  WAV+YKVDGFRFDLMGHIM+RTMVK
Sbjct: 505  LRRNTDGFIEHSTCVNNTASEHFMVERLIVDDLLHWAVDYKVDGFRFDLMGHIMRRTMVK 564

Query: 1645 AKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFNDRIRD 1824
            AK AL SL+K++ GVDGS IY+YGEGWDFGEVANN RGINASQFN+ GTGIGSFNDRIRD
Sbjct: 565  AKDALCSLTKERDGVDGSSIYIYGEGWDFGEVANNGRGINASQFNIHGTGIGSFNDRIRD 624

Query: 1825 AVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKDYILT 2004
            A+LGGSPFG+PLQQGF TGL L+PNG+D G ++V + ML+ S DHIQVG+AANL+D++LT
Sbjct: 625  AILGGSPFGHPLQQGFVTGLLLQPNGHDHGPEAVAEHMLAESKDHIQVGMAANLRDFVLT 684

Query: 2005 CHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVDERCR 2184
               G E+KGSEV+T+ G  V YTL P E +NYVSAHDNETLFDIVSLKTPME+SV+ERCR
Sbjct: 685  NCEGKEVKGSEVLTYGGTPVAYTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERCR 744

Query: 2185 MNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVGLPPR 2364
            +NHLATSIIAL QGIPFFHSGDE+LRSKSLDRDSYNSGDWFNR+DFTY SNNWGVGLPP+
Sbjct: 745  INHLATSIIALAQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPK 804

Query: 2365 EKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKERLRF 2544
            EKNE +WPL KPRLA+PSFKPQK HI+AAV+NF NLL+IRYSSPLFRLRTAN I+ER+RF
Sbjct: 805  EKNEKSWPLFKPRLADPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVRF 864

Query: 2545 HNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKTRKLQ 2724
            HNTGP  V GVI MS EDGH+G PG+ Q+DPIY+ +VVI NACPT++S   P L+ R LQ
Sbjct: 865  HNTGPSLVPGVIVMSIEDGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQARTLQ 924

Query: 2725 LHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            LHP Q+ S D++VK+S+Y+ ++G FTVP RTTSVFVE R
Sbjct: 925  LHPEQVMSTDEIVKRSTYNASAGCFTVPPRTTSVFVEPR 963


>gb|ONI23748.1| hypothetical protein PRUPE_2G205800 [Prunus persica]
          Length = 966

 Score = 1312 bits (3395), Expect = 0.0
 Identities = 629/880 (71%), Positives = 745/880 (84%), Gaps = 1/880 (0%)
 Frame = +1

Query: 205  LSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQSGLPE 384
            L Y+RA+WV+ S IAWN+     S +L AS+ AAL L  + I G DVK+ L  ++ GLPE
Sbjct: 85   LLYSRAFWVSESIIAWNVYVGNGSCYLLASKTAALSLTSDGILGEDVKVKLEEDKHGLPE 144

Query: 385  NVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDDIFAY 564
            NV +KFPHI  Y+AF +P  LDA+ LLKCQLAVA+F+ DG+C++AT LQ+ G+LDD+F+Y
Sbjct: 145  NVKEKFPHIKDYRAFNVPPDLDAKPLLKCQLAVATFNSDGRCSDATGLQLPGILDDLFSY 204

Query: 565  SGPLGAIFSKETVTLHLWAPTAQ-EVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGSRSW 741
            +GPLGA++SK++V+L+LWAPTAQ EV VC +++PS G  L+ V+LEE NGVWS  G +SW
Sbjct: 205  NGPLGALYSKQSVSLYLWAPTAQQEVCVCIYKEPSGGSPLEVVQLEEFNGVWSTKGPKSW 264

Query: 742  EGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNLANE 921
            EG YY+YEV VYHP+TL+IE C A DPYARGLSS+GRRT LVNLD +N+KP+GWD L +E
Sbjct: 265  EGCYYVYEVSVYHPSTLRIEKCYANDPYARGLSSDGRRTLLVNLDSDNIKPEGWDKLVDE 324

Query: 922  KPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGLTHI 1101
            KP++LS SDISIYELHIRDFSA+D +V  + RGGYLAFT QDS G  HLKKLSNAG+TH+
Sbjct: 325  KPDILSFSDISIYELHIRDFSASDQAVHPEFRGGYLAFTLQDSAGAIHLKKLSNAGITHV 384

Query: 1102 HLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVIWGV 1281
            HLLP+F FAGVDD+K NWK  D   + K PSDSDKQQA +TA+Q++DGYNWGYNPV+WGV
Sbjct: 385  HLLPAFQFAGVDDEKENWKSVDPEVLEKFPSDSDKQQALITAIQNDDGYNWGYNPVLWGV 444

Query: 1282 PKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIVPGY 1461
            PKGSYASN NG  R +EFRKMVQALNR GLRVVLDVVYNHL  SGP D+NSVLDKIVPGY
Sbjct: 445  PKGSYASNANGTYRAIEFRKMVQALNRFGLRVVLDVVYNHLHGSGPVDDNSVLDKIVPGY 504

Query: 1462 YLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKRTMV 1641
            YLRRNTDG IE+STC+NNTASEHFMVERLI+DDL  WAV+YKVDGFRFDLMGHIM+RTMV
Sbjct: 505  YLRRNTDGFIEHSTCVNNTASEHFMVERLIVDDLLHWAVDYKVDGFRFDLMGHIMRRTMV 564

Query: 1642 KAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFNDRIR 1821
            KAK AL SL+K++ GVDGS IY+YGEGWDFGEVANN RGINASQFN+ GTGIGSFNDRIR
Sbjct: 565  KAKDALCSLTKERDGVDGSSIYIYGEGWDFGEVANNGRGINASQFNIHGTGIGSFNDRIR 624

Query: 1822 DAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKDYIL 2001
            DA+LGGSPFG+PLQQGF TGL L+PNG+D G ++V + ML+ S DHIQVG+AANL+D++L
Sbjct: 625  DAILGGSPFGHPLQQGFVTGLLLQPNGHDHGPEAVAEHMLAESKDHIQVGMAANLRDFVL 684

Query: 2002 TCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVDERC 2181
            T   G E+KGSEV+T+ G  V YTL P E +NYVSAHDNETLFDIVSLKTPME+SV+ERC
Sbjct: 685  TNCEGKEVKGSEVLTYGGTPVAYTLCPTETINYVSAHDNETLFDIVSLKTPMEISVEERC 744

Query: 2182 RMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVGLPP 2361
            R+NHLATSIIAL QGIPFFHSGDE+LRSKSLDRDSYNSGDWFNR+DFTY SNNWGVGLPP
Sbjct: 745  RINHLATSIIALAQGIPFFHSGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPP 804

Query: 2362 REKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKERLR 2541
            +EKNE +WPL KPRLA+PSFKPQK HI+AAV+NF NLL+IRYSSPLFRLRTAN I+ER+R
Sbjct: 805  KEKNEKSWPLFKPRLADPSFKPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERVR 864

Query: 2542 FHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKTRKL 2721
            FHNTGP  V GVI MS EDGH+G PG+ Q+DPIY+ +VVI NACPT++S   P L+ R L
Sbjct: 865  FHNTGPSLVPGVIVMSIEDGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQARTL 924

Query: 2722 QLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            QLHP Q+ S D++VK+S+Y+ ++G FTVP RTTSVFVE R
Sbjct: 925  QLHPEQVMSTDEIVKRSTYNASAGCFTVPPRTTSVFVEPR 964


>gb|PHT29890.1| Pullulanase 1, chloroplastic [Capsicum baccatum]
          Length = 954

 Score = 1304 bits (3375), Expect = 0.0
 Identities = 620/883 (70%), Positives = 742/883 (84%)
 Frame = +1

Query: 193  ASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQS 372
            + D L Y+RA+WVT+S IAWN+G  E   +L+ASRKAAL + D+ IQG+DVKI L     
Sbjct: 72   SQDSLFYSRAFWVTKSIIAWNVGGGEDECYLYASRKAALCVDDDGIQGHDVKIKLERTNY 131

Query: 373  GLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDD 552
            GLP+ V +KFPHI  Y AF +P  LD ++L+KCQLAV  F+ DGKC +AT LQ+ G+LD+
Sbjct: 132  GLPQQVIEKFPHIRDYAAFEVPKTLDFQSLVKCQLAVGIFNSDGKCASATGLQLPGILDE 191

Query: 553  IFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGS 732
            +F+Y+GPLGAIF+ E V+L+LWAPTAQ V    ++ PS  D ++ V+L+EL+GVWSA G 
Sbjct: 192  LFSYTGPLGAIFATEAVSLYLWAPTAQAVQALIYKSPSEVDPVEIVQLKELDGVWSAKGP 251

Query: 733  RSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNL 912
            R WEG YY+YEV VYHP+TL+IE C + DPYARGLS++G+RT LVNLD +++KP+GWDNL
Sbjct: 252  RHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 311

Query: 913  ANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGL 1092
             +EKP+LLS SD+SIYELH+RDFSANDP+V  + +GGYLAFT QDS G+ HLKKLS+AG+
Sbjct: 312  QHEKPKLLSFSDVSIYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVQHLKKLSSAGV 371

Query: 1093 THIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVI 1272
            TH+HLLP+F FAGV+D+K  WK  D   +   P DS++QQA +T++QDEDGYNWGYNPV+
Sbjct: 372  THVHLLPTFQFAGVEDEKDKWKHIDIEILNSFPPDSEEQQALITSIQDEDGYNWGYNPVL 431

Query: 1273 WGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIV 1452
            WGVPKGSYASN NGP RI+EFRKMVQALNRIGLRVVLDVVYNHL ASG  D+ SVLDKIV
Sbjct: 432  WGVPKGSYASNANGPCRIVEFRKMVQALNRIGLRVVLDVVYNHLNASGHIDDFSVLDKIV 491

Query: 1453 PGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKR 1632
            PGY+LRRN DGGIE+STC+NNTASEHFMVERLILDDL  WAV+YKVDGFRFDLMGHIMKR
Sbjct: 492  PGYFLRRNVDGGIEHSTCVNNTASEHFMVERLILDDLKCWAVDYKVDGFRFDLMGHIMKR 551

Query: 1633 TMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFND 1812
            TM+KAK  L SLS +  GVDGS IY+YGEGWDFGEVANN RGINASQFNL G+GIGSFND
Sbjct: 552  TMLKAKSLLNSLSTENNGVDGSSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIGSFND 611

Query: 1813 RIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKD 1992
            RIRDA+LGGSPFG+PL QGF TGL LEPNG+D GD++ +  ML+ S DHIQVG+AANLKD
Sbjct: 612  RIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDEANVKRMLTVSTDHIQVGMAANLKD 671

Query: 1993 YILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVD 2172
            ++LT  +G ++KGS+V+ HDGK VGY  SPIE VNYVSAHDNETLFDI+SLKTP ++SV+
Sbjct: 672  FVLTNCDGQQVKGSDVLLHDGKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKDISVE 731

Query: 2173 ERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVG 2352
            ERCRMNHLATSIIAL+QGIPFFH+GDEMLRSKS+DRDSYNSGDWFNR+DF+Y SNNWGVG
Sbjct: 732  ERCRMNHLATSIIALSQGIPFFHAGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVG 791

Query: 2353 LPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKE 2532
            LPP+EKNE NWPLIKPRLA+PS+KPQK HILAAV+NF+NL++IRYSSPLFRLRTAN I+E
Sbjct: 792  LPPKEKNERNWPLIKPRLADPSYKPQKSHILAAVENFLNLMQIRYSSPLFRLRTANSIQE 851

Query: 2533 RLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKT 2712
            R+RFHNTGP W+ G+I MS EDGH   PG+ Q+DPIY+ +VVI N CPT +S   P L+ 
Sbjct: 852  RVRFHNTGPSWIPGLIVMSIEDGHQEVPGLSQLDPIYSYIVVIINPCPTDVSFANPALRE 911

Query: 2713 RKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            + LQLHPVQ+NS DD+VK S+YD +SG F VP RTTSVFVE R
Sbjct: 912  KSLQLHPVQMNSIDDVVKNSTYDASSGCFNVPARTTSVFVEPR 954


>ref|XP_019236141.1| PREDICTED: pullulanase 1, chloroplastic isoform X2 [Nicotiana
            attenuata]
 gb|OIT23986.1| pullulanase 1, chloroplastic [Nicotiana attenuata]
          Length = 955

 Score = 1304 bits (3375), Expect = 0.0
 Identities = 616/883 (69%), Positives = 740/883 (83%)
 Frame = +1

Query: 193  ASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQS 372
            + D L Y+RA+WV+RS IAWN+G  +   +L+AS+ AAL + D +IQG+DVKI L     
Sbjct: 73   SQDSLFYSRAFWVSRSIIAWNVGAGDGECYLYASKNAALCVADGEIQGHDVKIKLERTNY 132

Query: 373  GLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDD 552
            GLP+ V +KFPHI  Y AF +P+ L+ ++L+KCQLAV  F+ DGKC +AT LQ+ G+LD+
Sbjct: 133  GLPQQVVEKFPHIRDYTAFEVPATLNCQSLVKCQLAVGIFNSDGKCASATGLQLPGILDE 192

Query: 553  IFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGS 732
            +F+Y+GPLGA+F  E V+L+LWAPTAQEV    ++ P+  D ++ V+L+ELNGVWS  G 
Sbjct: 193  LFSYTGPLGAVFDTEAVSLYLWAPTAQEVRALIYKSPAEADPVEIVQLKELNGVWSVKGI 252

Query: 733  RSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNL 912
            R WEG YY+YEV VYHP+TL+IE C + DPYARGLS++G+RT LVNLD +++KP+GWDNL
Sbjct: 253  RHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 312

Query: 913  ANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGL 1092
             +EKP LLS SDIS+YELH+RDFSANDP+V  + +GGYLAFT QDS G+ HLK+LS+AG+
Sbjct: 313  QDEKPNLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGI 372

Query: 1093 THIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVI 1272
            TH+HLLP+F FAGV+D+K  WK  D  ++   P DS++QQA +TA+Q+EDGYNWGYNPV+
Sbjct: 373  THVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVL 432

Query: 1273 WGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIV 1452
            WGVPKGSYASN NGP RI+EFRKMVQALNRIGLRVV DVVYNH+ ASG  D+ SVLDKIV
Sbjct: 433  WGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVSDVVYNHINASGHFDDFSVLDKIV 492

Query: 1453 PGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKR 1632
            PGYYLRRN DGGIENSTC+NNTASEHFMVERLILDDL  WAV+YK+DGFRFDLMGHIMKR
Sbjct: 493  PGYYLRRNVDGGIENSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKR 552

Query: 1633 TMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFND 1812
            TMVKAK  L SLSKDK GVDG  IY+YGEGWDFGEVAN+ RGINASQFNL G+GIGSFND
Sbjct: 553  TMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANSGRGINASQFNLFGSGIGSFND 612

Query: 1813 RIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKD 1992
            RIRDA+LGGSPFG+PL QGF TGL LEPNG+D GDK+ ++ ML+ S DHIQVG+AANLKD
Sbjct: 613  RIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKD 672

Query: 1993 YILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVD 2172
            ++LT  +G E+KGSEV+ HD K VGY  SP+E VNYVSAHDNETLFDI+SLKTP ++SV+
Sbjct: 673  FVLTNCDGEEVKGSEVLLHDRKPVGYASSPVETVNYVSAHDNETLFDIISLKTPKDISVE 732

Query: 2173 ERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVG 2352
            ERCRMNHLATS+IAL+QGIPFFHSGDEMLRSKS+DRDSYNSGDWFNR+DF+Y SNNWGVG
Sbjct: 733  ERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVG 792

Query: 2353 LPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKE 2532
            LPP+EKNE NWPLIKPRLA+PS+KPQK HILAAV+NF NL++IRYSSPLFRLRTAN I+E
Sbjct: 793  LPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQE 852

Query: 2533 RLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKT 2712
            R+RFHNTGP W+ G+I MS EDGH G PG+ Q+DPIY+ +VVI N  PT +S   P L+ 
Sbjct: 853  RVRFHNTGPSWIPGLIVMSIEDGHQGVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRA 912

Query: 2713 RKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            R LQLHP+Q+NS DD+VK S+YD +SG F VP RTTSVFVE R
Sbjct: 913  RSLQLHPIQMNSTDDVVKNSTYDASSGCFNVPARTTSVFVEPR 955


>ref|XP_019236140.1| PREDICTED: pullulanase 1, chloroplastic isoform X1 [Nicotiana
            attenuata]
          Length = 957

 Score = 1304 bits (3374), Expect = 0.0
 Identities = 616/881 (69%), Positives = 739/881 (83%)
 Frame = +1

Query: 199  DFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQSGL 378
            D L Y+RA+WV+RS IAWN+G  +   +L+AS+ AAL + D +IQG+DVKI L     GL
Sbjct: 77   DSLFYSRAFWVSRSIIAWNVGAGDGECYLYASKNAALCVADGEIQGHDVKIKLERTNYGL 136

Query: 379  PENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDDIF 558
            P+ V +KFPHI  Y AF +P+ L+ ++L+KCQLAV  F+ DGKC +AT LQ+ G+LD++F
Sbjct: 137  PQQVVEKFPHIRDYTAFEVPATLNCQSLVKCQLAVGIFNSDGKCASATGLQLPGILDELF 196

Query: 559  AYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGSRS 738
            +Y+GPLGA+F  E V+L+LWAPTAQEV    ++ P+  D ++ V+L+ELNGVWS  G R 
Sbjct: 197  SYTGPLGAVFDTEAVSLYLWAPTAQEVRALIYKSPAEADPVEIVQLKELNGVWSVKGIRH 256

Query: 739  WEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNLAN 918
            WEG YY+YEV VYHP+TL+IE C + DPYARGLS++G+RT LVNLD +++KP+GWDNL +
Sbjct: 257  WEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNLQD 316

Query: 919  EKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGLTH 1098
            EKP LLS SDIS+YELH+RDFSANDP+V  + +GGYLAFT QDS G+ HLK+LS+AG+TH
Sbjct: 317  EKPNLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGITH 376

Query: 1099 IHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVIWG 1278
            +HLLP+F FAGV+D+K  WK  D  ++   P DS++QQA +TA+Q+EDGYNWGYNPV+WG
Sbjct: 377  VHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNEDGYNWGYNPVLWG 436

Query: 1279 VPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIVPG 1458
            VPKGSYASN NGP RI+EFRKMVQALNRIGLRVV DVVYNH+ ASG  D+ SVLDKIVPG
Sbjct: 437  VPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVSDVVYNHINASGHFDDFSVLDKIVPG 496

Query: 1459 YYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKRTM 1638
            YYLRRN DGGIENSTC+NNTASEHFMVERLILDDL  WAV+YK+DGFRFDLMGHIMKRTM
Sbjct: 497  YYLRRNVDGGIENSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKRTM 556

Query: 1639 VKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFNDRI 1818
            VKAK  L SLSKDK GVDG  IY+YGEGWDFGEVAN+ RGINASQFNL G+GIGSFNDRI
Sbjct: 557  VKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANSGRGINASQFNLFGSGIGSFNDRI 616

Query: 1819 RDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKDYI 1998
            RDA+LGGSPFG+PL QGF TGL LEPNG+D GDK+ ++ ML+ S DHIQVG+AANLKD++
Sbjct: 617  RDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKDFV 676

Query: 1999 LTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVDER 2178
            LT  +G E+KGSEV+ HD K VGY  SP+E VNYVSAHDNETLFDI+SLKTP ++SV+ER
Sbjct: 677  LTNCDGEEVKGSEVLLHDRKPVGYASSPVETVNYVSAHDNETLFDIISLKTPKDISVEER 736

Query: 2179 CRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVGLP 2358
            CRMNHLATS+IAL+QGIPFFHSGDEMLRSKS+DRDSYNSGDWFNR+DF+Y SNNWGVGLP
Sbjct: 737  CRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLP 796

Query: 2359 PREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKERL 2538
            P+EKNE NWPLIKPRLA+PS+KPQK HILAAV+NF NL++IRYSSPLFRLRTAN I+ER+
Sbjct: 797  PKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLMQIRYSSPLFRLRTANSIQERV 856

Query: 2539 RFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKTRK 2718
            RFHNTGP W+ G+I MS EDGH G PG+ Q+DPIY+ +VVI N  PT +S   P L+ R 
Sbjct: 857  RFHNTGPSWIPGLIVMSIEDGHQGVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRARS 916

Query: 2719 LQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            LQLHP+Q+NS DD+VK S+YD +SG F VP RTTSVFVE R
Sbjct: 917  LQLHPIQMNSTDDVVKNSTYDASSGCFNVPARTTSVFVEPR 957


>ref|XP_020703866.1| pullulanase 1, chloroplastic [Dendrobium catenatum]
          Length = 966

 Score = 1303 bits (3371), Expect = 0.0
 Identities = 623/904 (68%), Positives = 747/904 (82%)
 Frame = +1

Query: 130  IPFCSLSEAPMEETAVIQHPVASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAAL 309
            IP CS     M  ++  Q  ++   LS  +A+WV +S IAWN   D +SV L+AS +A L
Sbjct: 65   IPRCS----SMPNSSTDQRVLSQACLSNEKAFWVLKSLIAWNTSSDVASVCLYASWEAKL 120

Query: 310  FLLDEDIQGYDVKIPLVTEQSGLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVAS 489
             L D  ++G D KIPL  E+ GLP  V +KFPHI  YKA+ +P+ +D   +LKCQLA+AS
Sbjct: 121  CLTDRGLEGEDFKIPLEWERFGLPSKVLEKFPHIRDYKAYTVPASVDFRKILKCQLAIAS 180

Query: 490  FSGDGKCTNATALQISGVLDDIFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSS 669
            F  DGKC++ T +Q+ GVLDD+FAY GPLGAIFSK ++TL+LWAPTAQ V+   ++ PS+
Sbjct: 181  FGDDGKCSDVTGIQLPGVLDDLFAYDGPLGAIFSKRSITLNLWAPTAQVVHALLYKCPSA 240

Query: 670  GDLLQRVKLEELNGVWSAVGSRSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNG 849
            GD  + + L++ +GVW AVG RSWEG YYLY++ VYH +T+++ET T  DPYARG+SSNG
Sbjct: 241  GDPFECIHLKDQDGVWCAVGPRSWEGCYYLYQISVYHQSTVRVETYTVSDPYARGISSNG 300

Query: 850  RRTWLVNLDDENLKPDGWDNLANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYL 1029
             RT LV+L+ E+LKP GWD+LA EKP+L S SDISIYELHIRDFS ND SVD D RGGYL
Sbjct: 301  SRTLLVDLNSEDLKPSGWDDLAYEKPDLPSFSDISIYELHIRDFSENDQSVDPDFRGGYL 360

Query: 1030 AFTSQDSFGICHLKKLSNAGLTHIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQ 1209
            AFT QDS GI HLKKLSNAGLTH+HLLP F FAGVDDDKS WK  D+V M+KLP+DS++Q
Sbjct: 361  AFTCQDSLGINHLKKLSNAGLTHLHLLPCFQFAGVDDDKSQWKHTDDVMMSKLPADSEQQ 420

Query: 1210 QAEVTAVQDEDGYNWGYNPVIWGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDV 1389
            QA +T++QDEDGYNWGYNPV+WGVPKGSYASNPNGP RILEFRKM+QALN IGLRVVLDV
Sbjct: 421  QAIITSIQDEDGYNWGYNPVLWGVPKGSYASNPNGPCRILEFRKMIQALNHIGLRVVLDV 480

Query: 1390 VYNHLAASGPSDENSVLDKIVPGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTS 1569
            VYNH+ ASGP DE S+LDK+VPGYYLRR  +G IE+STCMNNTASEHFMVERLI+DDL  
Sbjct: 481  VYNHVHASGPLDETSILDKVVPGYYLRRTIEGCIEHSTCMNNTASEHFMVERLIIDDLLY 540

Query: 1570 WAVNYKVDGFRFDLMGHIMKRTMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANN 1749
            WA  YKVDGFRFDLMGH+MK TM++AK A+++L+KD  GVDGS IYLYGEGWDFGEVA N
Sbjct: 541  WATAYKVDGFRFDLMGHLMKGTMLRAKAAIENLTKDVDGVDGSKIYLYGEGWDFGEVAKN 600

Query: 1750 KRGINASQFNLPGTGIGSFNDRIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVM 1929
            KRGINASQ N+ G GIGSFNDR+RDA+LGGSPFG+PLQQGF TGL L+PN YD GDK ++
Sbjct: 601  KRGINASQLNITGNGIGSFNDRLRDAILGGSPFGHPLQQGFVTGLLLQPNEYDHGDKHIV 660

Query: 1930 DFMLSTSADHIQVGLAANLKDYILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSA 2109
            D MLSTSADHIQVGLAANL++++LT H G  MKG E++THDG  VGY LSP E VNYVSA
Sbjct: 661  DGMLSTSADHIQVGLAANLRNFVLTSHKGDVMKGCEILTHDGMPVGYALSPTETVNYVSA 720

Query: 2110 HDNETLFDIVSLKTPMELSVDERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSY 2289
            HDNETLFDI+ LK P+ +SVD+RCR+NH+ATSI+AL+QGIPFFH GD+MLRSKSLDRDSY
Sbjct: 721  HDNETLFDIICLKAPVGISVDDRCRINHVATSIVALSQGIPFFHCGDDMLRSKSLDRDSY 780

Query: 2290 NSGDWFNRIDFTYQSNNWGVGLPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVN 2469
            NSGDWFNR+DF+YQSNNWGVGLPPR KNE NWPLI+PRLA+ SFKPQKHHILAAV+NF+ 
Sbjct: 781  NSGDWFNRLDFSYQSNNWGVGLPPRGKNENNWPLIRPRLADSSFKPQKHHILAAVENFIE 840

Query: 2470 LLKIRYSSPLFRLRTANDIKERLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYAL 2649
            LL+IRYSS LFRL+TANDI+ER+RF NTG  WV+G+I MS ED ++GKPGI Q+D  Y+ 
Sbjct: 841  LLQIRYSSQLFRLQTANDIQERVRFLNTGTSWVQGLIVMSIEDCNEGKPGIVQLDHNYSY 900

Query: 2650 LVVIFNACPTQISLDVPELKTRKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVF 2829
            +V+IFNACPT++S+ +P LK R LQLHPV L+S D LVK+SSY+ +SG F +P+RTT+VF
Sbjct: 901  IVIIFNACPTEVSMPIPALKRRALQLHPVLLDSADSLVKRSSYESSSGCFKIPQRTTAVF 960

Query: 2830 VEVR 2841
            VEVR
Sbjct: 961  VEVR 964


>ref|XP_016436612.1| PREDICTED: pullulanase 1, chloroplastic-like [Nicotiana tabacum]
          Length = 952

 Score = 1301 bits (3366), Expect = 0.0
 Identities = 615/883 (69%), Positives = 740/883 (83%)
 Frame = +1

Query: 193  ASDFLSYARAYWVTRSHIAWNIGDDESSVWLFASRKAALFLLDEDIQGYDVKIPLVTEQS 372
            + D L Y+RA+WV+RS IAWN+G  E   +L+ASR AAL + D +IQG+DVKI L     
Sbjct: 70   SQDSLFYSRAFWVSRSIIAWNVGAGEGECYLYASRNAALCVADGEIQGHDVKIKLERTNY 129

Query: 373  GLPENVTQKFPHIATYKAFRLPSLLDAENLLKCQLAVASFSGDGKCTNATALQISGVLDD 552
            GL + V + FPHI  Y++F +P++L+ ++L+KCQLAV  F+ DGKC +AT LQ+ G+LD+
Sbjct: 130  GLSQQVVENFPHIRDYRSFEVPAILNCQSLVKCQLAVGIFNSDGKCASATGLQLPGILDE 189

Query: 553  IFAYSGPLGAIFSKETVTLHLWAPTAQEVNVCFFEDPSSGDLLQRVKLEELNGVWSAVGS 732
            +F+Y+GPLGA+F  E V+L+LWAPTAQEV    ++ PS  D ++ V+L+ELNGVWS  G 
Sbjct: 190  LFSYTGPLGAVFDTEAVSLYLWAPTAQEVRALIYKSPSEADPVEIVQLKELNGVWSVKGP 249

Query: 733  RSWEGLYYLYEVMVYHPTTLQIETCTAVDPYARGLSSNGRRTWLVNLDDENLKPDGWDNL 912
            R WEG YY+YEV VYHP+TL+IE C + DPYARGLS++G+RT LVNLD +++KP+GWDNL
Sbjct: 250  RHWEGCYYVYEVSVYHPSTLRIEKCVSNDPYARGLSADGKRTLLVNLDSDDVKPEGWDNL 309

Query: 913  ANEKPELLSLSDISIYELHIRDFSANDPSVDLDSRGGYLAFTSQDSFGICHLKKLSNAGL 1092
             +EKP LLS SDIS+YELH+RDFSANDP+V  + +GGYLAFT QDS G+ HLK+LS+AG+
Sbjct: 310  QDEKPNLLSFSDISLYELHVRDFSANDPTVPHEFQGGYLAFTLQDSAGVKHLKRLSSAGI 369

Query: 1093 THIHLLPSFHFAGVDDDKSNWKCPDEVEMAKLPSDSDKQQAEVTAVQDEDGYNWGYNPVI 1272
            TH+HLLP+F FAGV+D+K  WK  D  ++   P DS++QQA +TA+Q++DGYNWGYNPV+
Sbjct: 370  THVHLLPTFQFAGVEDEKHKWKHVDIEKLNSFPPDSEEQQALITAIQNKDGYNWGYNPVL 429

Query: 1273 WGVPKGSYASNPNGPNRILEFRKMVQALNRIGLRVVLDVVYNHLAASGPSDENSVLDKIV 1452
            WGVPKGSYASN NGP RI+EFRKMVQALNRIGLRVVLDVVYNH+ ASG  D+ SVLDKIV
Sbjct: 430  WGVPKGSYASNANGPCRIIEFRKMVQALNRIGLRVVLDVVYNHINASGHFDDFSVLDKIV 489

Query: 1453 PGYYLRRNTDGGIENSTCMNNTASEHFMVERLILDDLTSWAVNYKVDGFRFDLMGHIMKR 1632
            PGYYLRRN DGGIE+STC+NNTASEHFMVERLILDDL  WAV+YK+DGFRFDLMGHIMKR
Sbjct: 490  PGYYLRRNADGGIEHSTCVNNTASEHFMVERLILDDLKCWAVHYKIDGFRFDLMGHIMKR 549

Query: 1633 TMVKAKIALQSLSKDKAGVDGSMIYLYGEGWDFGEVANNKRGINASQFNLPGTGIGSFND 1812
            TMVKAK  L SLSKDK GVDG  IY+YGEGWDFGEVANN RGINASQFNL G+GIGSFND
Sbjct: 550  TMVKAKSLLNSLSKDKNGVDGPSIYIYGEGWDFGEVANNGRGINASQFNLFGSGIGSFND 609

Query: 1813 RIRDAVLGGSPFGNPLQQGFATGLSLEPNGYDQGDKSVMDFMLSTSADHIQVGLAANLKD 1992
            RIRDA+LGGSPFG+PL QGF TGL LEPNG+D GDK+ ++ ML+ S DHIQVG+AANLKD
Sbjct: 610  RIRDALLGGSPFGHPLHQGFVTGLYLEPNGHDLGDKANVERMLAVSKDHIQVGMAANLKD 669

Query: 1993 YILTCHNGVEMKGSEVMTHDGKSVGYTLSPIEIVNYVSAHDNETLFDIVSLKTPMELSVD 2172
            ++L+  +G E+KGSEV+ HD K VGY  SPIE VNYVSAHDNETLFDI+SLKTP ++SV+
Sbjct: 670  FVLSNCDGEEVKGSEVLLHDRKPVGYASSPIETVNYVSAHDNETLFDIISLKTPKDISVE 729

Query: 2173 ERCRMNHLATSIIALTQGIPFFHSGDEMLRSKSLDRDSYNSGDWFNRIDFTYQSNNWGVG 2352
            ERCRMNHLATS+IAL+QGIPFFHSGDEMLRSKS+DRDSYNSGDWFNR+DF+Y SNNWG G
Sbjct: 730  ERCRMNHLATSVIALSQGIPFFHSGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGGG 789

Query: 2353 LPPREKNEANWPLIKPRLANPSFKPQKHHILAAVDNFVNLLKIRYSSPLFRLRTANDIKE 2532
            LPP+EKNE NWPLIKPRLA+PS+KPQK HILAAV+NF NL++IRYSSPLFRLRTAN I+E
Sbjct: 790  LPPKEKNEWNWPLIKPRLADPSYKPQKSHILAAVENFSNLVQIRYSSPLFRLRTANSIQE 849

Query: 2533 RLRFHNTGPLWVRGVIAMSTEDGHDGKPGIPQMDPIYALLVVIFNACPTQISLDVPELKT 2712
            R+RFHNTGP W+ G+I MS EDGH G PG+ Q+DPIY+ +VVI N  PT +S   P L+ 
Sbjct: 850  RVRFHNTGPSWIPGLIVMSIEDGHQGVPGLSQLDPIYSYIVVIINPGPTDVSFANPALRA 909

Query: 2713 RKLQLHPVQLNSEDDLVKQSSYDPTSGSFTVPRRTTSVFVEVR 2841
            R LQLHP+Q+NS DD+VK S+YD +SG F VP RTTSVFVE R
Sbjct: 910  RSLQLHPIQMNSTDDVVKNSTYDASSGCFNVPTRTTSVFVEPR 952