BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00049954
         (300 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADU19849.1| putative secologanin synthase [Camptotheca acumin...   126   2e-27
gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]         118   4e-25
gb|AAA17732.1| cytochrome P450 [Catharanthus roseus]                  118   6e-25
sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Shor...   118   6e-25
gb|AEY75218.1| cytochrome P450 CYP72A129 [Panax ginseng]              105   4e-21

>gb|ADU19849.1| putative secologanin synthase [Camptotheca acuminata]
          Length = 524

 Score =  126 bits (316), Expect = 2e-27
 Identities = 54/99 (54%), Positives = 75/99 (75%)
 Frame = -2

Query: 299 PHIRKTVETYGNNSFMWFGRIPRVHITDPDLVREVLTKYYRFHKNLHDHDPITRVLLTGV 120
           PH+ KT++TYG NSF W GR+PRVHI +PDL++++L  +  F KN H ++P+T+ LLTG+
Sbjct: 86  PHVIKTIDTYGKNSFTWIGRMPRVHIMEPDLIKDILANHNDFMKNHHAYNPLTKFLLTGI 145

Query: 119 GMLEGEPWAARRKIIKSAFLFEKLKNMLPAFYVSSIEMM 3
           G LEG+ WA  R+II  +F  EKLK MLPAFYVS  +++
Sbjct: 146 GSLEGDKWAKHRRIISPSFHLEKLKTMLPAFYVSYDDLL 184


>gb|AAA17746.1| cytochrome P450, partial [Catharanthus roseus]
          Length = 516

 Score =  118 bits (296), Expect = 4e-25
 Identities = 51/99 (51%), Positives = 70/99 (70%)
 Frame = -2

Query: 299 PHIRKTVETYGNNSFMWFGRIPRVHITDPDLVREVLTKYYRFHKNLHDHDPITRVLLTGV 120
           PHI  T++TYG NSF W GRIPR+H+ +P+L++EVLT   ++ KN   H+P+ + LLTGV
Sbjct: 75  PHINHTIKTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTGV 134

Query: 119 GMLEGEPWAARRKIIKSAFLFEKLKNMLPAFYVSSIEMM 3
           G  EG  W+  R+II  AF  EKLK+MLPAF +   +M+
Sbjct: 135 GSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDML 173


>gb|AAA17732.1| cytochrome P450 [Catharanthus roseus]
          Length = 524

 Score =  118 bits (295), Expect = 6e-25
 Identities = 51/99 (51%), Positives = 69/99 (69%)
 Frame = -2

Query: 299 PHIRKTVETYGNNSFMWFGRIPRVHITDPDLVREVLTKYYRFHKNLHDHDPITRVLLTGV 120
           PHI  T+ TYG NSF W GRIPR+H+ +P+L++EVLT   ++ KN   H+P+ + LLTGV
Sbjct: 86  PHINHTINTYGGNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTGV 145

Query: 119 GMLEGEPWAARRKIIKSAFLFEKLKNMLPAFYVSSIEMM 3
           G  EG  W+  R+II  AF  EKLK+MLPAF +   +M+
Sbjct: 146 GSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDML 184


>sp|Q05047.1|C72A1_CATRO RecName: Full=Secologanin synthase; Short=SLS; AltName:
           Full=CYPLXXII; AltName: Full=Cytochrome P450 72A1
           gi|167484|gb|AAA33106.1| cytochrome P-450 protein
           [Catharanthus roseus] gi|445604|prf||1909351A cytochrome
           P450
          Length = 524

 Score =  118 bits (295), Expect = 6e-25
 Identities = 51/99 (51%), Positives = 69/99 (69%)
 Frame = -2

Query: 299 PHIRKTVETYGNNSFMWFGRIPRVHITDPDLVREVLTKYYRFHKNLHDHDPITRVLLTGV 120
           PHI  T+ TYG NSF W GRIPR+H+ +P+L++EVLT   ++ KN   H+P+ + LLTGV
Sbjct: 86  PHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHNPLVKFLLTGV 145

Query: 119 GMLEGEPWAARRKIIKSAFLFEKLKNMLPAFYVSSIEMM 3
           G  EG  W+  R+II  AF  EKLK+MLPAF +   +M+
Sbjct: 146 GSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDML 184


>gb|AEY75218.1| cytochrome P450 CYP72A129 [Panax ginseng]
          Length = 518

 Score =  105 bits (262), Expect = 4e-21
 Identities = 50/99 (50%), Positives = 69/99 (69%)
 Frame = -2

Query: 299 PHIRKTVETYGNNSFMWFGRIPRVHITDPDLVREVLTKYYRFHKNLHDHDPITRVLLTGV 120
           P  +K +  YG NSF+W G  P VHI +PD +++VL+K+Y+F K  H+  P+T++L TGV
Sbjct: 83  PLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRHN--PLTKLLATGV 140

Query: 119 GMLEGEPWAARRKIIKSAFLFEKLKNMLPAFYVSSIEMM 3
              EG+ WA  RK+I  AF  EKLKNMLPA Y+SS E++
Sbjct: 141 ADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIV 179