BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00015819
         (411 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus...   104   8e-21
sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase...   104   8e-21
ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]...   102   3e-20
ref|XP_002332458.1| cytochrome P450 [Populus trichocarpa] gi|222...    97   1e-18
emb|CBI39705.3| unnamed protein product [Vitis vinifera]               97   1e-18

>gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus roseus]
           gi|325989357|gb|ADZ48683.1| tabersonine 16-hydroxylase
           [synthetic construct]
          Length = 506

 Score =  104 bits (259), Expect = 8e-21
 Identities = 50/82 (60%), Positives = 63/82 (76%)
 Frame = -2

Query: 251 FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAVIKE 72
           F AG E S+T + WAM EMIKNPTV+ +AQ+EVRKV+ + GNVDE++LH+LKYLQAVIKE
Sbjct: 306 FSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDETKLHQLKYLQAVIKE 365

Query: 71  SMRLHXXXXXXXPRESSSQCEI 6
           ++RLH       PRE   QC+I
Sbjct: 366 TLRLHPPVPLLLPRECREQCKI 387


>sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase; AltName: Full=Cytochrome
           P450 71D12 gi|5921278|emb|CAB56503.1| cytochrome P450
           [Catharanthus roseus]
          Length = 495

 Score =  104 bits (259), Expect = 8e-21
 Identities = 50/82 (60%), Positives = 63/82 (76%)
 Frame = -2

Query: 251 FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAVIKE 72
           F AG E S+T + WAM EMIKNPTV+ +AQ+EVRKV+ + GNVDE++LH+LKYLQAVIKE
Sbjct: 295 FSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDETKLHQLKYLQAVIKE 354

Query: 71  SMRLHXXXXXXXPRESSSQCEI 6
           ++RLH       PRE   QC+I
Sbjct: 355 TLRLHPPVPLLLPRECREQCKI 376


>ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]
           gi|223537959|gb|EEF39572.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 499

 Score =  102 bits (254), Expect = 3e-20
 Identities = 48/83 (57%), Positives = 63/83 (75%)
 Frame = -2

Query: 251 FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAVIKE 72
           F AG E S+T + WAMSEM++NP V+ +AQ+EVR+V+G +GNVDE++LHEL YL+ VIKE
Sbjct: 300 FIAGSETSSTTVEWAMSEMLRNPRVMEKAQEEVRRVFGKKGNVDEAELHELNYLKLVIKE 359

Query: 71  SMRLHXXXXXXXPRESSSQCEIN 3
           ++RLH       PRES   CEIN
Sbjct: 360 TLRLHPPAPLLLPRESRESCEIN 382


>ref|XP_002332458.1| cytochrome P450 [Populus trichocarpa] gi|222832529|gb|EEE71006.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 490

 Score = 97.4 bits (241), Expect = 1e-18
 Identities = 46/83 (55%), Positives = 61/83 (73%)
 Frame = -2

Query: 251 FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAVIKE 72
           F AG + S+T +VWAMSEM+K+P V+ +AQ+EVR+V+GD+G VDE+ LHEL YL+  IKE
Sbjct: 286 FVAGTDTSSTTVVWAMSEMVKHPRVMKKAQEEVRQVFGDKGTVDEAGLHELNYLKLAIKE 345

Query: 71  SMRLHXXXXXXXPRESSSQCEIN 3
           + RLH       PRES   C+IN
Sbjct: 346 TFRLHPPVPLLLPRESREDCKIN 368


>emb|CBI39705.3| unnamed protein product [Vitis vinifera]
          Length = 1345

 Score = 97.1 bits (240), Expect = 1e-18
 Identities = 46/83 (55%), Positives = 61/83 (73%)
 Frame = -2

Query: 251 FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAVIKE 72
           F AG E S+TA+ WAM+EM+KNP V+A+AQ EVR ++  +GN DE+ +HELK+L+ VIKE
Sbjct: 545 FIAGSETSSTAVEWAMAEMLKNPGVMAKAQAEVRDIFSRKGNADETMIHELKFLKLVIKE 604

Query: 71  SMRLHXXXXXXXPRESSSQCEIN 3
           ++RLH       PRES   CEIN
Sbjct: 605 TLRLHPPVPLLIPRESRESCEIN 627



 Score = 81.6 bits (200), Expect = 6e-14
 Identities = 37/65 (56%), Positives = 52/65 (80%)
 Frame = -2

Query: 251 FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAVIKE 72
           F AG E S+TA+ WAM+EM+KNP V+A+AQ EVR ++  +GN DE+ + ELK+L+ VIKE
Sbjct: 267 FVAGTETSSTAVEWAMAEMLKNPRVMAKAQAEVRDIFSRKGNADETVVRELKFLKLVIKE 326

Query: 71  SMRLH 57
           ++RLH
Sbjct: 327 TLRLH 331



 Score = 65.1 bits (157), Expect = 6e-09
 Identities = 27/57 (47%), Positives = 44/57 (77%)
 Frame = -2

Query: 251  FGAGGEPSTTAIVWAMSEMIKNPTVLARAQDEVRKVYGDRGNVDESQLHELKYLQAV 81
            FGAG EPS+T I +AMSEM++NP ++ +AQ+EVR+++  +  +DE  + ELK+L+ +
Sbjct: 1171 FGAGSEPSSTTIDFAMSEMMRNPRIMRKAQEEVRRIFDRKEEIDEMGIQELKFLKLI 1227