BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00015752
         (1889 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADZ48682.1| cytochrome P450 [Catharanthus roseus]                  358   e-122
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   317   e-109
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                313   e-106
ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis ...   304   e-106
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                310   e-106

>gb|ADZ48682.1| cytochrome P450 [Catharanthus roseus]
          Length = 503

 Score =  358 bits (919), Expect(3) = e-122
 Identities = 191/356 (53%), Positives = 245/356 (68%), Gaps = 10/356 (2%)
 Frame = -3

Query: 1500 GPLISLRFGIRPVLLVFSPSLADECFTKTNDFVFANRPESISSKYLGYNGTTLVSSSYGD 1321
            GP++ LRFGIRP L+V SPS+ +E FTK ND  FANRP+SI+ K L YN TTL  S YG+
Sbjct: 64   GPIMLLRFGIRPTLIVSSPSIVEEIFTK-NDLAFANRPKSIALKLLQYNYTTLGFSPYGN 122

Query: 1320 HWRNLRRVATXXXXXXXXXXXXXXIWLEEIHFTVQKMF--SNNSDGKTWKAVNMNS*FRE 1147
            HWRNLRRVAT              IW EE+  + +++F  SN  + + W  +N+   F E
Sbjct: 123  HWRNLRRVATVHIFSSLNIFHSSNIWTEEVRLSAKQLFANSNKQNPEKWTELNLTDLFHE 182

Query: 1146 LVFNVIMKMVAGKRWLSDQSTDIF-TPQMIMDVSDYLPFLRWIGYKGQ--EKTMISLHRK 976
            LV+NV+M+M AGKRW   +S D F     ++D  D++P LRWIG+ G   EK M SL  +
Sbjct: 183  LVYNVMMRMAAGKRW--SKSADSFKVVNTLIDTYDFIPILRWIGFGGYKYEKEMRSLQDE 240

Query: 975  RDEFLQDLIDETRKGQGENGSCSAERKKTIIEALLELQVAEPEYYTDEIIKGIIPVVFAA 796
            RD+FLQDLIDE R  + E+ S   E KKT++++LL LQ A+P+YYTDEI+KG+I ++F A
Sbjct: 241  RDKFLQDLIDEGRINRNESNSID-EGKKTVVQSLLALQEADPQYYTDEIVKGMIQIMFTA 299

Query: 795  GTHTSAVTMERAMSFLLNHREALQKAKNEMDNNITQRGQLIEDSDLPKLPYLRCIIHETL 616
            GT  SAV M   MS LLNH E L+KAK E+ N I+  G+LI+D+DLP LPYLR II+E+L
Sbjct: 300  GTDISAVAMLWTMSLLLNHPEVLEKAKTEIKNQISPAGKLIDDADLPNLPYLRSIINESL 359

Query: 615  RLFPPAPTLLPHYSSEDCTIGGYRVPKGTTLLVNA*AIQRNPDFWE-----KPENF 463
            RL PPAP LLPH++SEDCTI GY VPK TT+L+N  AI R+P+ WE     KPE F
Sbjct: 360  RLCPPAPLLLPHFTSEDCTINGYNVPKETTVLINTWAIHRDPNVWEEATKFKPERF 415



 Score =  100 bits (250), Expect(3) = e-122
 Identities = 47/85 (55%), Positives = 65/85 (76%), Gaps = 1/85 (1%)
 Frame = -2

Query: 469 KFLPERFEGIEEGVFNE-GSKFIPFGKGRRACPGAAMAMRVVGLTLGNLIQCFEWERKVP 293
           KF PERFEGIE G  ++ G KF+ FGKG+R+CPG+ +A+R + LT+G LIQ F+W+R  P
Sbjct: 409 KFKPERFEGIEVGNSDQLGFKFVTFGKGKRSCPGSFLAVRFITLTIGTLIQSFDWKRLGP 468

Query: 292 EMVNLEEKSGSTLGKAKPLEALYKP 218
           E+V+++EK G T+ + KPL A YKP
Sbjct: 469 ELVDMQEKPGLTMHRDKPLVAFYKP 493



 Score = 29.6 bits (65), Expect(3) = e-122
 Identities = 14/27 (51%), Positives = 18/27 (66%)
 Frame = -2

Query: 1582 LPVLGHLHYFIKTAPQLALQKLSSKYG 1502
            LP++GHLH  +KT     LQ L+ KYG
Sbjct: 39   LPIIGHLH-LLKTPLHRTLQFLAEKYG 64


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  317 bits (812), Expect(2) = e-109
 Identities = 175/360 (48%), Positives = 234/360 (65%), Gaps = 14/360 (3%)
 Frame = -3

Query: 1500 GPLISLRFGIRPVLLVFSPSLADECFTKTNDFVFANRPESISSKYLGYNGTTLVSSSYGD 1321
            GP++  +FG RPVL+V SPS A+EC TK ND  FANRP  +  K+ G N TTL  +SYG 
Sbjct: 62   GPVLYFQFGSRPVLVVSSPSAAEECLTK-NDIGFANRPRLMIGKHSGNNYTTLAWASYGQ 120

Query: 1320 HWRNLRRVATXXXXXXXXXXXXXXIWLEEIHFTVQKMFSNNSDGKTWKAVNMNS*FRELV 1141
            +WRNLRR++               I  +E+   V+++ +    G  +  V M S F EL 
Sbjct: 121  NWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLVA----GYEYHKVEMKSVFFELT 176

Query: 1140 FNVIMKMVAGKRWLSDQSTDI-------------FTPQMIMDVSDYLPFLRWIGYKGQEK 1000
             N++M+M+AGKR+  +   D+             F      +V D+LP LRWIGYKG EK
Sbjct: 177  LNIMMRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEK 236

Query: 999  TMISLHRKRDEFLQDLIDETRKGQGENGSC-SAERKKTIIEALLELQVAEPEYYTDEIIK 823
             +I L  KRDEFLQ+LI E RK +  + S  S E+KKT+I+ LL LQ  EPEYYTDE+I+
Sbjct: 237  RLIRLKNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIR 296

Query: 822  GIIPVVFAAGTHTSAVTMERAMSFLLNHREALQKAKNEMDNNITQRGQLIEDSDLPKLPY 643
            G+I ++FAAGT TSA TME AMS LLN+ + ++KA++E+D  I + G+ I++SDL KLPY
Sbjct: 297  GLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQI-EPGRPIDESDLNKLPY 355

Query: 642  LRCIIHETLRLFPPAPTLLPHYSSEDCTIGGYRVPKGTTLLVNA*AIQRNPDFWEKPENF 463
            L CII+ETLR++P  P L+PH SSEDC IGGY +P GT LLVN  AIQ +P+ W++P  F
Sbjct: 356  LHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKF 415



 Score =  108 bits (270), Expect(2) = e-109
 Identities = 54/96 (56%), Positives = 66/96 (68%)
 Frame = -2

Query: 472 RKFLPERFEGIEEGVFNEGSKFIPFGKGRRACPGAAMAMRVVGLTLGNLIQCFEWERKVP 293
           RKF PERFEG + GV  +G K +PFG GRR CPG  +A RVVGLTL +L+QCF+W R   
Sbjct: 413 RKFKPERFEGYQGGV-RDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGE 471

Query: 292 EMVNLEEKSGSTLGKAKPLEALYKPRLATSNFISQL 185
           EMV + E +G TL K  PLEA  +PR    NF+SQ+
Sbjct: 472 EMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNFLSQV 507


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  313 bits (801), Expect(2) = e-106
 Identities = 168/360 (46%), Positives = 224/360 (62%), Gaps = 14/360 (3%)
 Frame = -3

Query: 1500 GPLISLRFGIRPVLLVFSPSLADECFTKTNDFVFANRPESISSKYLGYNGTTLVSSSYGD 1321
            GP++ L+ G R VL+V SPS A+ECFTK ND VFANRP+ +  K +G N T+L  S YGD
Sbjct: 77   GPILRLQLGFRRVLIVSSPSAAEECFTK-NDIVFANRPKMLFGKIIGVNYTSLAWSPYGD 135

Query: 1320 HWRNLRRVATXXXXXXXXXXXXXXIWLEEIHFTVQKMFSNNSDGKTWKAVNMNS*FRELV 1141
            +WRNLRR+A+              I +EE    +QK+ S  + G +   V M   F EL 
Sbjct: 136  NWRNLRRIASIEILSIHRLNEFHDIRVEETRLLIQKLLSACNSGSS--QVTMKFSFYELT 193

Query: 1140 FNVIMKMVAGKRWLS--------------DQSTDIFTPQMIMDVSDYLPFLRWIGYKGQE 1003
             NV+M+M++GKR+                D   + F      +V DYLP L W+G KG E
Sbjct: 194  LNVMMRMISGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLE 253

Query: 1002 KTMISLHRKRDEFLQDLIDETRKGQGENGSCSAERKKTIIEALLELQVAEPEYYTDEIIK 823
            K +I L  KRD F Q LID+ RK +G        +KKT+IE LL LQ  EPEYYTD +I+
Sbjct: 254  KKLIKLQEKRDVFFQGLIDQLRKSKGTE---DVNKKKTMIELLLSLQETEPEYYTDAMIR 310

Query: 822  GIIPVVFAAGTHTSAVTMERAMSFLLNHREALQKAKNEMDNNITQRGQLIEDSDLPKLPY 643
              + V+ AAG+ TSA TME  MS LLNH + L+KA+NE+D+ +  +  L+++SD+P LPY
Sbjct: 311  SFVLVLLAAGSDTSAGTMEWVMSLLLNHPQVLKKAQNEIDS-VIGKNCLVDESDIPNLPY 369

Query: 642  LRCIIHETLRLFPPAPTLLPHYSSEDCTIGGYRVPKGTTLLVNA*AIQRNPDFWEKPENF 463
            LRCII+ETLRL+P  P L+PH +S DC +GGY VP+GT L+VN  AI  +P  W++PE F
Sbjct: 370  LRCIINETLRLYPAGPLLVPHEASSDCVVGGYNVPRGTILIVNQWAIHHDPKVWDEPETF 429



 Score =  102 bits (255), Expect(2) = e-106
 Identities = 48/94 (51%), Positives = 68/94 (72%)
 Frame = -2

Query: 466 FLPERFEGIEEGVFNEGSKFIPFGKGRRACPGAAMAMRVVGLTLGNLIQCFEWERKVPEM 287
           F PERFEG+E     +G K +PFG GRR+CPG  +A+R++G+TLG++IQCF+WER   E+
Sbjct: 429 FKPERFEGLEGT--RDGFKLLPFGSGRRSCPGEGLAVRMLGMTLGSIIQCFDWERTSEEL 486

Query: 286 VNLEEKSGSTLGKAKPLEALYKPRLATSNFISQL 185
           V++ E  G T+ KA PL A  KPR+  +N +S+L
Sbjct: 487 VDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLSEL 520


>ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis vinifera]
          Length = 507

 Score =  304 bits (779), Expect(2) = e-106
 Identities = 161/367 (43%), Positives = 235/367 (64%), Gaps = 18/367 (4%)
 Frame = -3

Query: 1509 SMAGPLISLRFGIRPVLLVFSPSLADECFTKTNDFVFANRPESISSKYLGYNGTTLVSSS 1330
            S  GP++ LRFG RP ++V SPS   ECFTK ND V ANRP S+   ++ YN T    +S
Sbjct: 61   SQYGPILFLRFGCRPFVVVSSPSAVQECFTK-NDVVLANRPRSMIGDHVTYNYTAFAWAS 119

Query: 1329 YGDHWRNLRRVATXXXXXXXXXXXXXXIWLEEIHFTVQKMFSNNSDGKTWKAVNMNS*FR 1150
            YG  WR LRR+                +  EE+ + ++++F  ++DG   + V+M     
Sbjct: 120  YGHLWRVLRRLTVVEILSSNKLLLLSTVREEEVRYLLRQLFKVSNDGA--QKVDMRLYLS 177

Query: 1149 ELVFNVIMKMVAGKRWLSDQSTDI-------------FTPQMIMDVSDYLPFLRWIGYKG 1009
               FN IMK + GKR + +++  I             F P    ++ D+ P LRW+GYKG
Sbjct: 178  LFSFNFIMKTITGKRCIEEEAEGIETNRQFLERLKRIFVPTTTTNLCDFFPILRWVGYKG 237

Query: 1008 QEKTMISLHRKRDEFLQDLIDETRKGQGENGSCSAERKKTIIEALLELQVAEPEYYTDEI 829
             EK++I   ++RD +LQ ++DE R+    N +   ++K+T+IE LL LQ +EP++YTD++
Sbjct: 238  LEKSVIQFGKERDGYLQGMLDEFRRN---NSAVEWQKKRTLIETLLFLQQSEPDFYTDDV 294

Query: 828  IKGIIPVVFAAGTHTSAVTMERAMSFLLNHREALQKAKNEMDNNITQRGQLIEDSDLPKL 649
            IKG++ VV +AGT TS+VT+E AMS LLNH EAL+KA+ E+D+++ + G L++DSDL KL
Sbjct: 295  IKGLMLVVISAGTDTSSVTLEWAMSLLLNHPEALEKARAEIDSHV-KPGHLLDDSDLAKL 353

Query: 648  PYLRCIIHETLRLFPPAPTLLPHYSSEDCTIGGYRVPKGTTLLVNA*AIQRNPDFWE--- 478
            PYLR +++ETLRL+P AP LLPH SSEDC++GG+ +P+GTT++VN  A+ R+P  WE   
Sbjct: 354  PYLRSVVNETLRLYPTAPLLLPHLSSEDCSVGGFDIPRGTTVMVNVWALHRDPRVWEEAT 413

Query: 477  --KPENF 463
              KPE F
Sbjct: 414  KFKPERF 420



 Score =  111 bits (277), Expect(2) = e-106
 Identities = 55/95 (57%), Positives = 68/95 (71%)
 Frame = -2

Query: 469 KFLPERFEGIEEGVFNEGSKFIPFGKGRRACPGAAMAMRVVGLTLGNLIQCFEWERKVPE 290
           KF PERFEG+E     E  KF PFG GRRACPGAA+AM++V L LG LIQCFEWER   E
Sbjct: 414 KFKPERFEGMENEE-KEAFKFAPFGIGRRACPGAALAMKIVSLALGGLIQCFEWERVEAE 472

Query: 289 MVNLEEKSGSTLGKAKPLEALYKPRLATSNFISQL 185
            V++   SG T+ KAKPLE +++PR   ++ +SQL
Sbjct: 473 KVDMSPGSGITMPKAKPLEIIFRPRPTMTSLLSQL 507


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  310 bits (793), Expect(2) = e-106
 Identities = 167/360 (46%), Positives = 223/360 (61%), Gaps = 14/360 (3%)
 Frame = -3

Query: 1500 GPLISLRFGIRPVLLVFSPSLADECFTKTNDFVFANRPESISSKYLGYNGTTLVSSSYGD 1321
            G ++ L+ G R VL+V SPS A+ECFTK ND VFANRP+ +  K +G N T+L  S YGD
Sbjct: 62   GQILRLQLGFRRVLIVSSPSAAEECFTK-NDIVFANRPKMLFGKIIGVNYTSLAWSPYGD 120

Query: 1320 HWRNLRRVATXXXXXXXXXXXXXXIWLEEIHFTVQKMFSNNSDGKTWKAVNMNS*FRELV 1141
            +WRNLRR+A+              I +EE    +QK+ S  + G +   V M   F EL 
Sbjct: 121  NWRNLRRIASIEILSIHRLNEFHDIRVEETRLLIQKLLSACNSGSS--QVTMKFSFYELT 178

Query: 1140 FNVIMKMVAGKRWLS--------------DQSTDIFTPQMIMDVSDYLPFLRWIGYKGQE 1003
             NV+M+M++GKR+                D   + F      +V DYLP L W+G KG E
Sbjct: 179  LNVMMRMISGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLE 238

Query: 1002 KTMISLHRKRDEFLQDLIDETRKGQGENGSCSAERKKTIIEALLELQVAEPEYYTDEIIK 823
            K +I L  KRD F Q LID+ RK +G        +KKT+IE LL LQ  EPEYYTD +I+
Sbjct: 239  KKLIKLQEKRDVFFQGLIDQLRKSKGTE---DVNKKKTMIELLLSLQETEPEYYTDAMIR 295

Query: 822  GIIPVVFAAGTHTSAVTMERAMSFLLNHREALQKAKNEMDNNITQRGQLIEDSDLPKLPY 643
              + V+ AAG+ TSA TME  MS LLNH + L+KA+NE+D+ +  +  L+++SD+P LPY
Sbjct: 296  SFVLVLLAAGSDTSAGTMEWVMSLLLNHPQVLKKAQNEIDS-VIGKNCLVDESDIPNLPY 354

Query: 642  LRCIIHETLRLFPPAPTLLPHYSSEDCTIGGYRVPKGTTLLVNA*AIQRNPDFWEKPENF 463
            LRCII+ETLRL+P  P L+PH +S DC +GGY VP+GT L+VN  AI  +P  W++PE F
Sbjct: 355  LRCIINETLRLYPAGPLLVPHEASSDCVVGGYNVPRGTILIVNQWAIHHDPKVWDEPETF 414



 Score =  102 bits (255), Expect(2) = e-106
 Identities = 48/94 (51%), Positives = 68/94 (72%)
 Frame = -2

Query: 466 FLPERFEGIEEGVFNEGSKFIPFGKGRRACPGAAMAMRVVGLTLGNLIQCFEWERKVPEM 287
           F PERFEG+E     +G K +PFG GRR+CPG  +A+R++G+TLG++IQCF+WER   E+
Sbjct: 414 FKPERFEGLEGT--RDGFKLLPFGSGRRSCPGEGLAVRMLGMTLGSIIQCFDWERTSEEL 471

Query: 286 VNLEEKSGSTLGKAKPLEALYKPRLATSNFISQL 185
           V++ E  G T+ KA PL A  KPR+  +N +S+L
Sbjct: 472 VDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLSEL 505