BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00006053
         (3821 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAC56914.1| nitrate transporter [Nicotiana tabacum]               830   0.0  
emb|CAC00544.1| putative low-affinity nitrate transporter [Nicot...   829   0.0  
dbj|BAC56913.1| nitrate transporter [Nicotiana tabacum]               828   0.0  
dbj|BAC56915.1| nitrate transporter [Nicotiana tabacum]               820   0.0  
dbj|BAC56916.1| nitrate transporter [Nicotiana tabacum]               818   0.0  

>dbj|BAC56914.1| nitrate transporter [Nicotiana tabacum]
          Length = 590

 Score =  830 bits (2143), Expect = 0.0
 Identities = 415/594 (69%), Positives = 485/594 (81%), Gaps = 2/594 (0%)
 Frame = +2

Query: 56   MALPQTHADAKTVPDAWDYKGRRALHSTTGGWHSAAMILGVEACERLTTLGIAINLVTYL 235
            MALP+T  D KT+PDAWDYKGR A+ S++GGW SAAMILG+EA ERLTTLGIA+NLVTYL
Sbjct: 1    MALPETQQDTKTLPDAWDYKGRPAVRSSSGGWSSAAMILGIEAVERLTTLGIAVNLVTYL 60

Query: 236  TGTMHLGNATSANTVTNFLGTSFMLCLLGGFIADTYLGRYLTIAIFAPVQAMGVTVLTIS 415
            TGTMHLGNA+SAN VTNFLGTSFML LLGGF+ADT+LGRYLTI IF  +QAMGVT+LTIS
Sbjct: 61   TGTMHLGNASSANNVTNFLGTSFMLTLLGGFVADTFLGRYLTIGIFTTIQAMGVTILTIS 120

Query: 416  TIVPSLRPPKCAAGSSSCVPASGNQLLVLYISLYLTALGTGGLKXXXXXXXXXXXXXXXX 595
            TI+PSLRPPKC+ GSS+C+PAS  QL+VLYI+LY+TALGTGGLK                
Sbjct: 121  TIIPSLRPPKCSPGSSTCIPASSKQLMVLYIALYMTALGTGGLKSSVSGFGSDQFDETDK 180

Query: 596  KERTRMIKXXXXXXXXISVGSLMAVTVLVYIQDNVGRRWGYGICAFAIVGGWVVFLLGTR 775
            KER +MIK        I+VGSL AVTVLVYIQDN+GR +GYGICA AIV G V+FL GTR
Sbjct: 181  KERGQMIKFFNWFFFFINVGSLGAVTVLVYIQDNLGREYGYGICACAIVIGLVIFLSGTR 240

Query: 776  RYRFKKLVGSPLTQFASVFVAALNKRHLELPSDSSLLFNIDDIPMEANNXXXXXXLPHSK 955
            +YRFKKLVGSPLTQ ASVFVAA NKRH+ELPSDSSLL+NIDDIP + N       LPHSK
Sbjct: 241  KYRFKKLVGSPLTQIASVFVAAWNKRHMELPSDSSLLYNIDDIPGDGNKKAKQR-LPHSK 299

Query: 956  QFRFLDKAAIR--DTEMPRDLNMINKWQLATLTDVEEVKMVIRMLPIWATTIFFWTIHAQ 1129
            +FRFLDKAAI+  D E    + ++NKW L+TLTDVEEVK+V+RMLP WATTI FWT++AQ
Sbjct: 300  EFRFLDKAAIKVQDPESA-GITVVNKWNLSTLTDVEEVKLVVRMLPTWATTIMFWTVYAQ 358

Query: 1130 MTTFTVSQASTMHRKVGTFEIPAAAMAGFFIGSILITVPVYDRFIMPACKKLFKNPHGLT 1309
            MTTF+VSQA+TM R +G FEIP A++  FF+GSIL+T   YDR ++P C+++  NPHG +
Sbjct: 359  MTTFSVSQATTMDRHIGNFEIPPASLTLFFVGSILLTCIFYDRAVVPVCRRVLNNPHGTS 418

Query: 1310 PLQRIGIGLVLAIASMSVAALVEIKRLKVARLHGLTNSGAATVPISVFWIVPQFSFVGAG 1489
            PLQRI +GL+L+I +M  AAL E+KRL VA LHGLTN   A VP+SVFW+VPQF  VGAG
Sbjct: 419  PLQRIAVGLILSIIAMIAAALTEVKRLNVAHLHGLTNDANAKVPLSVFWLVPQFLLVGAG 478

Query: 1490 EAFTYVGQLDFFLKECPKGMKTMSTGLFLSTCSIGFFFSSVLVSIVHKVTAHKTPWLADN 1669
            EAFTY+GQLDFFL+ECPKGMKTMSTGLFLST S+GFFFSS+LV+IVHKVT  K PWLADN
Sbjct: 479  EAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSILVTIVHKVTG-KNPWLADN 537

Query: 1670 LNRGKLYDFYWLLAILCALNLMAFLVCAKGYVYKEKRLADQGIELEETSEPVCH 1831
            LN+G+LYDFYWLLA L  LNLM FL  ++ YVYKEKRLA+ GIE+E+ SEP CH
Sbjct: 538  LNQGRLYDFYWLLATLSVLNLMIFLFISRRYVYKEKRLAECGIEMED-SEPACH 590


>emb|CAC00544.1| putative low-affinity nitrate transporter [Nicotiana plumbaginifolia]
          Length = 590

 Score =  829 bits (2141), Expect = 0.0
 Identities = 414/594 (69%), Positives = 485/594 (81%), Gaps = 2/594 (0%)
 Frame = +2

Query: 56   MALPQTHADAKTVPDAWDYKGRRALHSTTGGWHSAAMILGVEACERLTTLGIAINLVTYL 235
            MALP+T  D KT+PDAWDYKGR A+ S++GGW SAAMILG+EA ERLTTLGIA+NLVTYL
Sbjct: 1    MALPETQQDTKTLPDAWDYKGRPAVRSSSGGWSSAAMILGIEAVERLTTLGIAVNLVTYL 60

Query: 236  TGTMHLGNATSANTVTNFLGTSFMLCLLGGFIADTYLGRYLTIAIFAPVQAMGVTVLTIS 415
            TGTMHLGNA+SAN VTNFLGTSFML LLGGF+ADT+LGRYLTI IF  +QAMGVT+LTIS
Sbjct: 61   TGTMHLGNASSANNVTNFLGTSFMLTLLGGFVADTFLGRYLTIGIFTTIQAMGVTILTIS 120

Query: 416  TIVPSLRPPKCAAGSSSCVPASGNQLLVLYISLYLTALGTGGLKXXXXXXXXXXXXXXXX 595
            TI+PSLRPPKC+ GSS+C+PAS  QL+VLYI+LY+TALGTGGLK                
Sbjct: 121  TIIPSLRPPKCSPGSSTCIPASSKQLMVLYIALYMTALGTGGLKSSVSGFGSDQFDETDK 180

Query: 596  KERTRMIKXXXXXXXXISVGSLMAVTVLVYIQDNVGRRWGYGICAFAIVGGWVVFLLGTR 775
            KER +MIK        I+VGSL AVTVLVYIQDN+GR +GYGICA AIV G V+FL GTR
Sbjct: 181  KERGQMIKFFNWFFFFINVGSLGAVTVLVYIQDNLGREYGYGICACAIVIGLVIFLSGTR 240

Query: 776  RYRFKKLVGSPLTQFASVFVAALNKRHLELPSDSSLLFNIDDIPMEANNXXXXXXLPHSK 955
            +YRFKKLVGSPLTQ ASVFVAA NKRH++LPSDS+LL+NIDDIP + N       LPHSK
Sbjct: 241  KYRFKKLVGSPLTQIASVFVAAWNKRHMDLPSDSTLLYNIDDIPGDGNKKAKQR-LPHSK 299

Query: 956  QFRFLDKAAIR--DTEMPRDLNMINKWQLATLTDVEEVKMVIRMLPIWATTIFFWTIHAQ 1129
            +FRFLDKAAI+  DTE    + ++NKW L+TLTDVEEVK+V+RMLP WATTI FWT++AQ
Sbjct: 300  EFRFLDKAAIKVQDTESA-GITVVNKWNLSTLTDVEEVKLVVRMLPTWATTIMFWTVYAQ 358

Query: 1130 MTTFTVSQASTMHRKVGTFEIPAAAMAGFFIGSILITVPVYDRFIMPACKKLFKNPHGLT 1309
            MTTF+VSQA+TM R +G FEIP A++  FF+GSIL+T   YDR ++P C+++  NPHG T
Sbjct: 359  MTTFSVSQATTMDRHIGNFEIPPASLTLFFVGSILLTCIFYDRAVVPVCRRVLNNPHGTT 418

Query: 1310 PLQRIGIGLVLAIASMSVAALVEIKRLKVARLHGLTNSGAATVPISVFWIVPQFSFVGAG 1489
            PLQRI +GL+L+I +M  AAL E+KRL VA LHGLTN   A VP+SVFW+VPQF  VGAG
Sbjct: 419  PLQRIAVGLILSIIAMVAAALTEVKRLNVAHLHGLTNDANAKVPLSVFWLVPQFLLVGAG 478

Query: 1490 EAFTYVGQLDFFLKECPKGMKTMSTGLFLSTCSIGFFFSSVLVSIVHKVTAHKTPWLADN 1669
            EAFTY+GQLDFFL+ECPKGMKTMSTGLFLST S+GFF SS+LV+IVHKVT  K PWLADN
Sbjct: 479  EAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFVSSILVTIVHKVTV-KNPWLADN 537

Query: 1670 LNRGKLYDFYWLLAILCALNLMAFLVCAKGYVYKEKRLADQGIELEETSEPVCH 1831
            LN+G+LYDFYWLLA L  LNLM FL  ++ YVYKEKRLA+ GIE+E+ SEP CH
Sbjct: 538  LNQGRLYDFYWLLATLSVLNLMVFLFISRRYVYKEKRLAECGIEMED-SEPACH 590


>dbj|BAC56913.1| nitrate transporter [Nicotiana tabacum]
          Length = 590

 Score =  828 bits (2138), Expect = 0.0
 Identities = 414/594 (69%), Positives = 484/594 (81%), Gaps = 2/594 (0%)
 Frame = +2

Query: 56   MALPQTHADAKTVPDAWDYKGRRALHSTTGGWHSAAMILGVEACERLTTLGIAINLVTYL 235
            MALP+T  D KT+PDAWDYKGR A+ S++GGW SAAMILG+EA ERLTTLGIA+NLVTYL
Sbjct: 1    MALPETQQDTKTLPDAWDYKGRPAVRSSSGGWSSAAMILGIEAVERLTTLGIAVNLVTYL 60

Query: 236  TGTMHLGNATSANTVTNFLGTSFMLCLLGGFIADTYLGRYLTIAIFAPVQAMGVTVLTIS 415
            TGTMHLGNA+SAN VTNFLGTSFML LLGGF+ADT+LGRYLTI IF  +QAMGVT+LTIS
Sbjct: 61   TGTMHLGNASSANNVTNFLGTSFMLTLLGGFVADTFLGRYLTIGIFTTIQAMGVTILTIS 120

Query: 416  TIVPSLRPPKCAAGSSSCVPASGNQLLVLYISLYLTALGTGGLKXXXXXXXXXXXXXXXX 595
            TI+PSLRPPKC+ GSS+C+PAS  QL+VLYI+LY+TALGTGGLK                
Sbjct: 121  TIIPSLRPPKCSPGSSTCIPASSKQLMVLYIALYMTALGTGGLKSSVSGFGSDQFDETDK 180

Query: 596  KERTRMIKXXXXXXXXISVGSLMAVTVLVYIQDNVGRRWGYGICAFAIVGGWVVFLLGTR 775
            KER +MIK        I+VGSL AVTVLVYIQDN+GR +GYGICA AIV G V+FL GTR
Sbjct: 181  KERGQMIKFFNWFFFFINVGSLGAVTVLVYIQDNLGREYGYGICACAIVIGLVIFLSGTR 240

Query: 776  RYRFKKLVGSPLTQFASVFVAALNKRHLELPSDSSLLFNIDDIPMEANNXXXXXXLPHSK 955
            +YRFKKLVGSPLTQ ASVFVAA NKRH++LPSDS LL+NIDDIP + N       LPHSK
Sbjct: 241  KYRFKKLVGSPLTQIASVFVAAWNKRHMDLPSDSYLLYNIDDIPGDGNKKAKQR-LPHSK 299

Query: 956  QFRFLDKAAIR--DTEMPRDLNMINKWQLATLTDVEEVKMVIRMLPIWATTIFFWTIHAQ 1129
            +FRFLDKAAI+  D E    + ++NKW L+TLTDVEEVK+V+RMLP WATTI FWT++AQ
Sbjct: 300  EFRFLDKAAIKVQDPESA-GITVVNKWNLSTLTDVEEVKLVVRMLPTWATTIMFWTVYAQ 358

Query: 1130 MTTFTVSQASTMHRKVGTFEIPAAAMAGFFIGSILITVPVYDRFIMPACKKLFKNPHGLT 1309
            MTTF+VSQA+TM R +G FEIP A++  FF+GSIL+T   YDR ++P C+++  NPHG T
Sbjct: 359  MTTFSVSQATTMDRHIGNFEIPPASLTLFFVGSILLTCIFYDRAVVPVCRRVLNNPHGTT 418

Query: 1310 PLQRIGIGLVLAIASMSVAALVEIKRLKVARLHGLTNSGAATVPISVFWIVPQFSFVGAG 1489
            PLQRI +GL+L+I +M  AAL E+KRL VA LHGLTN   A VP+SVFW+VPQF  VGAG
Sbjct: 419  PLQRIAVGLILSIIAMVAAALTEVKRLNVAHLHGLTNDANAKVPLSVFWLVPQFLLVGAG 478

Query: 1490 EAFTYVGQLDFFLKECPKGMKTMSTGLFLSTCSIGFFFSSVLVSIVHKVTAHKTPWLADN 1669
            EAFTY+GQLDFFL+ECPKGMKTMSTGLFLST S+GFFFSS+LV+IVHKVT  K PWLADN
Sbjct: 479  EAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSILVTIVHKVTV-KNPWLADN 537

Query: 1670 LNRGKLYDFYWLLAILCALNLMAFLVCAKGYVYKEKRLADQGIELEETSEPVCH 1831
            LN+G+LYDFYWLLA L  LNLM FL  ++ YVYKEKRLA+ GIE+E+ SEP CH
Sbjct: 538  LNQGRLYDFYWLLATLSVLNLMIFLFISRRYVYKEKRLAECGIEMED-SEPACH 590


>dbj|BAC56915.1| nitrate transporter [Nicotiana tabacum]
          Length = 594

 Score =  820 bits (2117), Expect = 0.0
 Identities = 420/596 (70%), Positives = 478/596 (80%), Gaps = 4/596 (0%)
 Frame = +2

Query: 56   MALPQTHADAKTVPDAWDYKGRRALHSTTGGWHSAAMILGVEACERLTTLGIAINLVTYL 235
            MALP+T  D+K +PDAWDYKGR AL S++GGW S AMILGVEA ERLTTLGIA+NLVTYL
Sbjct: 1    MALPETQQDSKALPDAWDYKGRPALRSSSGGWASGAMILGVEAVERLTTLGIAVNLVTYL 60

Query: 236  TGTMHLGNATSANTVTNFLGTSFMLCLLGGFIADTYLGRYLTIAIFAPVQAMGVTVLTIS 415
            TGTMHLGNAT+AN VTNFLGTSFML L GGF+ADT+LGRYLTI IFA VQAMGVT+LTIS
Sbjct: 61   TGTMHLGNATAANNVTNFLGTSFMLTLFGGFVADTFLGRYLTIGIFATVQAMGVTILTIS 120

Query: 416  TIVPSLRPPKCA-AGSSSCVPASGNQLLVLYISLYLTALGTGGLKXXXXXXXXXXXXXXX 592
            TI+PSLRPPKC   GSSSC+PA+  QL+VLYI+LY+TALGTGGLK               
Sbjct: 121  TIIPSLRPPKCEQVGSSSCIPANSKQLMVLYIALYMTALGTGGLKSSVSGFGTDQFDDAD 180

Query: 593  XKERTRMIKXXXXXXXXISVGSLMAVTVLVYIQDNVGRRWGYGICAFAIVGGWVVFLLGT 772
             KE+ +MIK        I+VGSL AVTVLVYIQDN+GR WGYGICA AIV G VVFL GT
Sbjct: 181  KKEKGQMIKFFDWFFFFINVGSLGAVTVLVYIQDNLGREWGYGICACAIVIGLVVFLSGT 240

Query: 773  RRYRFKKLVGSPLTQFASVFVAALNKRHLELPSDSSLLFNIDDIPMEANNXXXXXXLPHS 952
            R+YRFKKLVGSPLTQ ASV VAA  KRHLEL SDSSLLF IDDI  E N       LPHS
Sbjct: 241  RKYRFKKLVGSPLTQIASVIVAAWKKRHLELLSDSSLLFEIDDIFGEGNKKNKQK-LPHS 299

Query: 953  KQFRFLDKAAIR---DTEMPRDLNMINKWQLATLTDVEEVKMVIRMLPIWATTIFFWTIH 1123
            K++RFLDKAAI+   D E      +INKW+LATLTDVEEVK++IRMLP WATTI FWT++
Sbjct: 300  KEYRFLDKAAIKEDHDLESNGTNVVINKWKLATLTDVEEVKLLIRMLPTWATTIMFWTVY 359

Query: 1124 AQMTTFTVSQASTMHRKVGTFEIPAAAMAGFFIGSILITVPVYDRFIMPACKKLFKNPHG 1303
            AQMTTF+VSQA+TM R +GTFEIP A++  FF+GSIL+TV  YDR I+P C++    PHG
Sbjct: 360  AQMTTFSVSQATTMDRHIGTFEIPPASLTVFFVGSILLTVIFYDRVIVPICRRFMNKPHG 419

Query: 1304 LTPLQRIGIGLVLAIASMSVAALVEIKRLKVARLHGLTNSGAATVPISVFWIVPQFSFVG 1483
            LTPLQRI  GLVL+I +M  AAL E+KRLKVA LHGLTN   AT+P+SVFW+VPQF  VG
Sbjct: 420  LTPLQRIFTGLVLSILAMIAAALTEVKRLKVAHLHGLTNDANATIPLSVFWLVPQFLLVG 479

Query: 1484 AGEAFTYVGQLDFFLKECPKGMKTMSTGLFLSTCSIGFFFSSVLVSIVHKVTAHKTPWLA 1663
            AGEAFTY+GQLDFFL+ECPKGMKTMSTGLFLST ++GFFFSS+LV+IVH VT    PWLA
Sbjct: 480  AGEAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSILVTIVHVVTGTTNPWLA 539

Query: 1664 DNLNRGKLYDFYWLLAILCALNLMAFLVCAKGYVYKEKRLADQGIELEETSEPVCH 1831
            DNLN+G+LYDFYWLLAIL  LNLM FL  ++ YVYKEKRLA+ GIELE+   PVCH
Sbjct: 540  DNLNQGRLYDFYWLLAILSVLNLMFFLYFSRKYVYKEKRLAEMGIELEDDG-PVCH 594


>dbj|BAC56916.1| nitrate transporter [Nicotiana tabacum]
          Length = 594

 Score =  818 bits (2112), Expect = 0.0
 Identities = 418/596 (70%), Positives = 476/596 (79%), Gaps = 4/596 (0%)
 Frame = +2

Query: 56   MALPQTHADAKTVPDAWDYKGRRALHSTTGGWHSAAMILGVEACERLTTLGIAINLVTYL 235
            MALP+T  D+K +PDAWDYKGR AL S++GGW S AMILGVEA ERLTTLGIA+NLVTYL
Sbjct: 1    MALPETQQDSKALPDAWDYKGRPALRSSSGGWASGAMILGVEAVERLTTLGIAVNLVTYL 60

Query: 236  TGTMHLGNATSANTVTNFLGTSFMLCLLGGFIADTYLGRYLTIAIFAPVQAMGVTVLTIS 415
            TGTMHLGNA+SAN VTNFLGTSFML LLGGF+ADT+LGRYLTI IFA VQAMGVT+LTIS
Sbjct: 61   TGTMHLGNASSANNVTNFLGTSFMLTLLGGFVADTFLGRYLTIGIFATVQAMGVTILTIS 120

Query: 416  TIVPSLRPPKCA-AGSSSCVPASGNQLLVLYISLYLTALGTGGLKXXXXXXXXXXXXXXX 592
            TI+PSLRPPKC   GSSSC+PA+  QL+VLYI+LY+TALGTGGLK               
Sbjct: 121  TIIPSLRPPKCEQVGSSSCIPANSKQLMVLYIALYMTALGTGGLKSSVSGFGTDQFDDSD 180

Query: 593  XKERTRMIKXXXXXXXXISVGSLMAVTVLVYIQDNVGRRWGYGICAFAIVGGWVVFLLGT 772
             KE+ +MIK        I+VGSL AVTVLVYIQDN+GR WGYGICA AIV   VVFL GT
Sbjct: 181  KKEKGQMIKFFDWFFFFINVGSLGAVTVLVYIQDNLGREWGYGICACAIVIALVVFLFGT 240

Query: 773  RRYRFKKLVGSPLTQFASVFVAALNKRHLELPSDSSLLFNIDDIPMEANNXXXXXXLPHS 952
            R+YRFKKL GSPLTQ ASV VAA  KRHLELPSDSSLLF IDDI  E N       LPHS
Sbjct: 241  RKYRFKKLGGSPLTQIASVIVAAWKKRHLELPSDSSLLFEIDDIFGEGNKKSKQK-LPHS 299

Query: 953  KQFRFLDKAAIR---DTEMPRDLNMINKWQLATLTDVEEVKMVIRMLPIWATTIFFWTIH 1123
            K++RFLDKAAI+   D E      +INKW+LATLTDVEEVK++IRMLP WATTI FWT++
Sbjct: 300  KEYRFLDKAAIKEDDDLESNGTNVVINKWKLATLTDVEEVKILIRMLPTWATTIMFWTVY 359

Query: 1124 AQMTTFTVSQASTMHRKVGTFEIPAAAMAGFFIGSILITVPVYDRFIMPACKKLFKNPHG 1303
            AQMTTF+VSQA+TM R +GTFEIP A +  FF+GSIL+TV  YDR I+P C++    PHG
Sbjct: 360  AQMTTFSVSQATTMDRHIGTFEIPPATLTVFFVGSILLTVIFYDRIIVPICRRFMNKPHG 419

Query: 1304 LTPLQRIGIGLVLAIASMSVAALVEIKRLKVARLHGLTNSGAATVPISVFWIVPQFSFVG 1483
            LTPLQRI  GLVL+I +M  AAL E+KRLKVA LHGLTN   AT+P++VFW+VPQF  VG
Sbjct: 420  LTPLQRIFTGLVLSILAMIAAALTEVKRLKVAHLHGLTNDANATIPLTVFWLVPQFLLVG 479

Query: 1484 AGEAFTYVGQLDFFLKECPKGMKTMSTGLFLSTCSIGFFFSSVLVSIVHKVTAHKTPWLA 1663
            AGEAFTY+GQLDFFL+ECPKGMKTMSTGLFLST ++GFFFSS+LV+IVH VT    PWLA
Sbjct: 480  AGEAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLALGFFFSSILVTIVHVVTGTTNPWLA 539

Query: 1664 DNLNRGKLYDFYWLLAILCALNLMAFLVCAKGYVYKEKRLADQGIELEETSEPVCH 1831
            DNLN+G+LYDFYWLLAIL   NLM FL  ++ YVYKEKRLA+ GIELE+   PVCH
Sbjct: 540  DNLNQGRLYDFYWLLAILSVFNLMFFLYSSRKYVYKEKRLAEMGIELEDDG-PVCH 594