BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00005914
         (845 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus...   285   9e-75
sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase...   285   9e-75
ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   283   3e-74
ref|XP_003617703.1| Cytochrome P450 [Medicago truncatula] gi|355...   280   3e-73
ref|XP_003615863.1| Cytochrome P450 [Medicago truncatula] gi|355...   280   4e-73

>gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus roseus]
            gi|325989357|gb|ADZ48683.1| tabersonine 16-hydroxylase
            [synthetic construct]
          Length = 506

 Score =  285 bits (729), Expect = 9e-75
 Identities = 141/289 (48%), Positives = 203/289 (70%), Gaps = 8/289 (2%)
 Frame = +2

Query: 2    EVMNMIKAISAQKGSVVNLSRKVSSLTYSMIAQAAFGKRSEYHDEFIALMDEVVKLMGGF 181
            EV+N IK+I +++G+ +NLS+++S L Y +  +AAFG++++  +EFI L+D++ K +   
Sbjct: 158  EVLNFIKSIGSKEGTRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEP 217

Query: 182  CLVDLYPSVKILEKITGIRKKLEKTRVRVDAILQSILTEHKNK--------RGESKPGNE 337
             + D++PS+K L+ I+  + K+EK   + D I+++IL  HK K         GE K   E
Sbjct: 218  NIADMFPSLKFLQLISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKK---E 274

Query: 338  DLVDVLVNVQKSGEFGDTLTDDNLKGVIYDIFTAGGETSSTTVIWAFLEMLKNPTVLKKA 517
            DLVDVL+N+Q+  +F   L D N+K +I++IF+AG ETSSTTV WA  EM+KNPTV+KKA
Sbjct: 275  DLVDVLLNIQRRNDFEAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKA 334

Query: 518  QDEVRAVFGSRGNADESQLDELKYMHAVVKETXXXXXXXXXXXXXESSEKCEINGYTIPA 697
            Q+EVR VF   GN DE++L +LKY+ AV+KET             E  E+C+I GYTIP+
Sbjct: 335  QEEVRKVFNEEGNVDETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPS 394

Query: 698  KTRVIVNAFAIARDPTHWKEPEKFNPDRFLEAEIDYKGKNLFYIPFGAG 844
            K+RVIVNA+AI RDP +W EPEKFNPDRFLE+++D+KG +  Y+PFG G
Sbjct: 395  KSRVIVNAWAIGRDPNYWIEPEKFNPDRFLESKVDFKGNSFEYLPFGGG 443


>sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase; AltName: Full=Cytochrome
            P450 71D12 gi|5921278|emb|CAB56503.1| cytochrome P450
            [Catharanthus roseus]
          Length = 495

 Score =  285 bits (729), Expect = 9e-75
 Identities = 141/289 (48%), Positives = 203/289 (70%), Gaps = 8/289 (2%)
 Frame = +2

Query: 2    EVMNMIKAISAQKGSVVNLSRKVSSLTYSMIAQAAFGKRSEYHDEFIALMDEVVKLMGGF 181
            EV+N IK+I +++G+ +NLS+++S L Y +  +AAFG++++  +EFI L+D++ K +   
Sbjct: 147  EVLNFIKSIGSKEGTRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEP 206

Query: 182  CLVDLYPSVKILEKITGIRKKLEKTRVRVDAILQSILTEHKNK--------RGESKPGNE 337
             + D++PS+K L+ I+  + K+EK   + D I+++IL  HK K         GE K   E
Sbjct: 207  NIADMFPSLKFLQLISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKK---E 263

Query: 338  DLVDVLVNVQKSGEFGDTLTDDNLKGVIYDIFTAGGETSSTTVIWAFLEMLKNPTVLKKA 517
            DLVDVL+N+Q+  +F   L D N+K +I++IF+AG ETSSTTV WA  EM+KNPTV+KKA
Sbjct: 264  DLVDVLLNIQRRNDFEAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKA 323

Query: 518  QDEVRAVFGSRGNADESQLDELKYMHAVVKETXXXXXXXXXXXXXESSEKCEINGYTIPA 697
            Q+EVR VF   GN DE++L +LKY+ AV+KET             E  E+C+I GYTIP+
Sbjct: 324  QEEVRKVFNEEGNVDETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPS 383

Query: 698  KTRVIVNAFAIARDPTHWKEPEKFNPDRFLEAEIDYKGKNLFYIPFGAG 844
            K+RVIVNA+AI RDP +W EPEKFNPDRFLE+++D+KG +  Y+PFG G
Sbjct: 384  KSRVIVNAWAIGRDPNYWIEPEKFNPDRFLESKVDFKGNSFEYLPFGGG 432


>ref|XP_003634756.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 71D10-like [Vitis
            vinifera]
          Length = 554

 Score =  283 bits (725), Expect = 3e-74
 Identities = 140/285 (49%), Positives = 197/285 (69%), Gaps = 4/285 (1%)
 Frame = +2

Query: 2    EVMNMIKAISAQKGSVVNLSRKVSSLTYSMIAQAAFGKRSEYHDEFIALMDEVVKLMGGF 181
            EV  +I +IS   GS +NLS K+ SLTY + +++AFGK+    D F++ + E V+L  GF
Sbjct: 203  EVSKLIWSISLNAGSPINLSEKIFSLTYGITSRSAFGKKFRGQDAFVSAILEAVELSAGF 262

Query: 182  CLVDLYPSVKILEKITGIRKKLEKTRVRVDAILQSILTEHKNKRGESKPGN----EDLVD 349
            C+ D+YPS+K L  I+G++ KLEK   ++D IL +I+ +H+ ++  +K G     EDLVD
Sbjct: 263  CVADMYPSLKWLHYISGMKPKLEKVHQKIDRILNNIIDDHRKRKTTTKAGQPETQEDLVD 322

Query: 350  VLVNVQKSGEFGDTLTDDNLKGVIYDIFTAGGETSSTTVIWAFLEMLKNPTVLKKAQDEV 529
            VL+N+Q+ G+ G  LTD N+K V+ DIF+ GGETSST V+WA  EMLK+P V++KAQ EV
Sbjct: 323  VLLNLQEHGDLGIPLTDGNVKAVLLDIFSGGGETSSTAVVWAMAEMLKSPIVMEKAQAEV 382

Query: 530  RAVFGSRGNADESQLDELKYMHAVVKETXXXXXXXXXXXXXESSEKCEINGYTIPAKTRV 709
            R VF  + + +E+ + ELKY+++VVKET             E  E+C INGY IP  T+V
Sbjct: 383  RRVFDGKRDINETGIHELKYLNSVVKETLRLHPSVPLLLPRECRERCVINGYEIPENTKV 442

Query: 710  IVNAFAIARDPTHWKEPEKFNPDRFLEAEIDYKGKNLFYIPFGAG 844
            I+NA+AIA+DP HW EP KF P+RFL++ ID+KG +  YIPFGAG
Sbjct: 443  IINAWAIAQDPDHWFEPNKFFPERFLDSSIDFKGTDFKYIPFGAG 487


>ref|XP_003617703.1| Cytochrome P450 [Medicago truncatula] gi|355519038|gb|AET00662.1|
            Cytochrome P450 [Medicago truncatula]
          Length = 746

 Score =  280 bits (716), Expect = 3e-73
 Identities = 138/282 (48%), Positives = 195/282 (69%), Gaps = 1/282 (0%)
 Frame = +2

Query: 2    EVMNMIKAISAQKGSVVNLSRKVSSLTYSMIAQAAFGKRSEYHDEFIALMDEVVKLMGGF 181
            EV N+  +I+A +GS+VNL+ K+ S+TY +  +AAFGKRS++   F + ++E+V L+GG 
Sbjct: 403  EVSNLATSITASEGSIVNLTHKIFSMTYGITTRAAFGKRSKHQQSFKSAVEEIVSLLGGI 462

Query: 182  CLVDLYPSVKILEKITGIRKKLEKTRVRVDAILQSILTEHKNKRGESKPGNEDLVDVLVN 361
            C+VDLYPS+K+L+ ++  + K+EK    +D ILQ I+ +HK+   E     E LVDVL+ 
Sbjct: 463  CIVDLYPSIKLLQCVSRAKAKMEKLHKEIDMILQDIIDDHKSIHKEDS-NEEYLVDVLLK 521

Query: 362  VQKSGEFGD-TLTDDNLKGVIYDIFTAGGETSSTTVIWAFLEMLKNPTVLKKAQDEVRAV 538
            +Q+        LTDDN+K +I DIF AG ETSSTTV+WA  EM+KNP V+++AQ EVR V
Sbjct: 522  IQQENYHSQHPLTDDNIKSIIQDIFDAGTETSSTTVLWAISEMVKNPKVMEEAQAEVRRV 581

Query: 539  FGSRGNADESQLDELKYMHAVVKETXXXXXXXXXXXXXESSEKCEINGYTIPAKTRVIVN 718
            F  +G  DE++L +L Y+ +V+KET             ES E+C+INGY IPAKTRV+VN
Sbjct: 582  FDRKGFVDETELHQLIYLKSVIKETMRLHPTVPLLLPRESRERCQINGYEIPAKTRVMVN 641

Query: 719  AFAIARDPTHWKEPEKFNPDRFLEAEIDYKGKNLFYIPFGAG 844
            A+AI RDP +W + E F P+RF+ + ID+KG +  YIPFGAG
Sbjct: 642  AWAIGRDPRYWVDAESFKPERFVNSPIDFKGTDFEYIPFGAG 683



 Score = 74.7 bits (182), Expect = 2e-11
 Identities = 39/90 (43%), Positives = 61/90 (67%)
 Frame = +2

Query: 2   EVMNMIKAISAQKGSVVNLSRKVSSLTYSMIAQAAFGKRSEYHDEFIALMDEVVKLMGGF 181
           E+++  +  S  +GSVVNLS K+ S+TY + A+A FGKR+ + D FI  +++VV L+G F
Sbjct: 142 ELLSAKRIQSFSEGSVVNLSEKIFSMTYGITARAVFGKRNRHQDVFIPALEKVVVLLGRF 201

Query: 182 CLVDLYPSVKILEKITGIRKKLEKTRVRVD 271
            + DLYPS+K+L+ +T      EKT+ R +
Sbjct: 202 EIADLYPSIKLLQWMT-----REKTKNRFE 226


>ref|XP_003615863.1| Cytochrome P450 [Medicago truncatula] gi|355517198|gb|AES98821.1|
           Cytochrome P450 [Medicago truncatula]
          Length = 491

 Score =  280 bits (715), Expect = 4e-73
 Identities = 135/283 (47%), Positives = 194/283 (68%), Gaps = 3/283 (1%)
 Frame = +2

Query: 5   VMNMIKAISAQKGSVVNLSRKVSSLTYSMIAQAAFGKRSEYHDEFIALMDEVVKLMGGFC 184
           V N+IK+IS  KGSVVNLS K+ ++T ++  +AAFGKR+++   F + + E+  LMGGFC
Sbjct: 148 VSNLIKSISTSKGSVVNLSHKIFAMTSAITTRAAFGKRNKHQQVFQSAIKEIASLMGGFC 207

Query: 185 LVDLYPSVKILEKITGIRKKLEKTRVRVDAILQSILTEHKN-KRGESKPGNEDLVDVLVN 361
           + D+YPS+K+L++++G++ K EK    +D ILQ I+ +HKN  + ESK  +EDLVD L+ 
Sbjct: 208 IADVYPSIKMLQRVSGVKTKFEKFHKEIDMILQDIVDDHKNIHKEESK--DEDLVDALIK 265

Query: 362 VQKSGEFGD--TLTDDNLKGVIYDIFTAGGETSSTTVIWAFLEMLKNPTVLKKAQDEVRA 535
           +Q+  +     TLTDD++K +I D+F  G ETSS  V+W   EM+KNP ++K+AQ EVR 
Sbjct: 266 IQQENDLSHDHTLTDDSMKSIILDMFVGGTETSSGVVLWGISEMIKNPKIMKEAQAEVRK 325

Query: 536 VFGSRGNADESQLDELKYMHAVVKETXXXXXXXXXXXXXESSEKCEINGYTIPAKTRVIV 715
           VF  +G+ DE++L  L Y+ +++KET             ES E+C+INGY IPAKTRV +
Sbjct: 326 VFDKKGHVDETELHHLIYLKSIIKETLRLHPSLPLLIPRESRERCQINGYEIPAKTRVAI 385

Query: 716 NAFAIARDPTHWKEPEKFNPDRFLEAEIDYKGKNLFYIPFGAG 844
           N +AI RD  +W E E F P+RF+ + ID+KG N  YIPFGAG
Sbjct: 386 NVWAIGRDERYWAEAESFKPERFVNSTIDFKGTNFEYIPFGAG 428