BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cephaelis21_contig00005913
         (1094 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]...   400   e-109
gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus...   394   e-107
sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase...   394   e-107
ref|XP_003519623.1| PREDICTED: cytochrome P450 71D10-like [Glyci...   391   e-106
ref|XP_002332458.1| cytochrome P450 [Populus trichocarpa] gi|222...   389   e-106

>ref|XP_002522721.1| cytochrome P450, putative [Ricinus communis]
            gi|223537959|gb|EEF39572.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 499

 Score =  400 bits (1027), Expect = e-109
 Identities = 194/363 (53%), Positives = 260/363 (71%)
 Frame = +1

Query: 1    QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXXMSDHDIIFANRPPLLAA 180
            QLLG LPH  L DLAKKYG +MH+QLG                M  HDI+FA RP LLAA
Sbjct: 48   QLLGSLPHHRLRDLAKKYGSIMHLQLGEVPHVVVSSPEAAKEVMKTHDIVFAQRPFLLAA 107

Query: 181  CVFTHNCQDIAFSPYGNYWRQMRKLCATELLSSKRVQTFGSIREKEVMNMIKSISSRKGS 360
             V T+N  DIAFSPY +YWRQ+RK+C  ELLS+KRVQ+F SIRE+EV N+I +++S  G 
Sbjct: 108  SVITYNFTDIAFSPYSDYWRQLRKICVLELLSAKRVQSFRSIREEEVSNLINTLTSFSGK 167

Query: 361  VINLSKRIYTLSYSIIAEAAFGKGSKYTEEFIPLMEVVLKLFGGFSLVDMYPSVKILERI 540
              N S+++++ +Y I A A+FGK  K  EEFI + + + ++ GGFSL D++PS+  L  I
Sbjct: 168  PFNFSRKLFSSTYGIAARASFGKKYKDQEEFIKVAQQMTEVAGGFSLADLFPSIAFLHVI 227

Query: 541  TGVRNKLERTQEQLDAVLESILNEHKNRGAESEAGNQEAKEDLVDVLLNVQKSGEFGAPL 720
            +G++++L R ++  D +LE+I+++H+ R    +   +   +DLVDVLL  QK G+   PL
Sbjct: 228  SGMKSRLLRLRDDGDNILENIIDDHRARNMTGKTSGEGEDDDLVDVLLRFQKGGQLEFPL 287

Query: 721  SDNSIKAVIYDIFSAGGDTSATTVTWAMFEMIKNPTMLERAQDEVRKVFGGRGNADESQL 900
            + N+IKAVI DIF AG +TS+TTV WAM EM++NP ++E+AQ+EVR+VFG +GN DE++L
Sbjct: 288  TTNNIKAVILDIFIAGSETSSTTVEWAMSEMLRNPRVMEKAQEEVRRVFGKKGNVDEAEL 347

Query: 901  HELKYLQAVIKETLRLHAPVPLLLPRESSEQCEINGYVIPAKTRVIINCWAMGRDPMYWT 1080
            HEL YL+ VIKETLRLH P PLLLPRES E CEINGY IP  ++VI+N WA+GRDP  WT
Sbjct: 348  HELNYLKLVIKETLRLHPPAPLLLPRESRESCEINGYYIPVNSKVIVNAWAIGRDPNNWT 407

Query: 1081 DPE 1089
            + E
Sbjct: 408  EAE 410


>gb|ACM92061.1| tabersonine 16-hydroxylase CYP71D12 [Catharanthus roseus]
            gi|325989357|gb|ADZ48683.1| tabersonine 16-hydroxylase
            [synthetic construct]
          Length = 506

 Score =  394 bits (1011), Expect = e-107
 Identities = 199/366 (54%), Positives = 261/366 (71%), Gaps = 2/366 (0%)
 Frame = +1

Query: 1    QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXXMSDHDIIFANRPPLLAA 180
            QL G   H IL DLAKKYGPLMH+++G                   HDI+FA+RP  L +
Sbjct: 52   QLSGGHTHHILRDLAKKYGPLMHLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLES 111

Query: 181  C-VFTHNCQDIAFSPYGNYWRQMRKLCATELLSSKRVQTFGSIREKEVMNMIKSISSRKG 357
              + +++  D+  SPYGNYWRQ+RK+   ELLS K VQ+F SIRE+EV+N IKSI S++G
Sbjct: 112  FKIVSYDFSDMVVSPYGNYWRQLRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEG 171

Query: 358  SVINLSKRIYTLSYSIIAEAAFGKGSKYTEEFIPLMEVVLKLFGGFSLVDMYPSVKILER 537
            + INLSK I  L Y I   AAFG+ +K TEEFI L++ + K     ++ DM+PS+K L+ 
Sbjct: 172  TRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQL 231

Query: 538  ITGVRNKLERTQEQLDAVLESILNEHKNR-GAESEAGNQEAKEDLVDVLLNVQKSGEFGA 714
            I+  + K+E+  +Q D ++E+IL  HK +        N E KEDLVDVLLN+Q+  +F A
Sbjct: 232  ISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKKEDLVDVLLNIQRRNDFEA 291

Query: 715  PLSDNSIKAVIYDIFSAGGDTSATTVTWAMFEMIKNPTMLERAQDEVRKVFGGRGNADES 894
            PL D +IKA+I++IFSAG +TS+TTV WAM EMIKNPT++++AQ+EVRKVF   GN DE+
Sbjct: 292  PLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDET 351

Query: 895  QLHELKYLQAVIKETLRLHAPVPLLLPRESSEQCEINGYVIPAKTRVIINCWAMGRDPMY 1074
            +LH+LKYLQAVIKETLRLH PVPLLLPRE  EQC+I GY IP+K+RVI+N WA+GRDP Y
Sbjct: 352  KLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDPNY 411

Query: 1075 WTDPER 1092
            W +PE+
Sbjct: 412  WIEPEK 417


>sp|P98183.1|C71DC_CATRO RecName: Full=Tabersonine 16-hydroxylase; AltName: Full=Cytochrome
            P450 71D12 gi|5921278|emb|CAB56503.1| cytochrome P450
            [Catharanthus roseus]
          Length = 495

 Score =  394 bits (1011), Expect = e-107
 Identities = 199/366 (54%), Positives = 261/366 (71%), Gaps = 2/366 (0%)
 Frame = +1

Query: 1    QLLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXXMSDHDIIFANRPPLLAA 180
            QL G   H IL DLAKKYGPLMH+++G                   HDI+FA+RP  L +
Sbjct: 41   QLSGGHTHHILRDLAKKYGPLMHLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLES 100

Query: 181  C-VFTHNCQDIAFSPYGNYWRQMRKLCATELLSSKRVQTFGSIREKEVMNMIKSISSRKG 357
              + +++  D+  SPYGNYWRQ+RK+   ELLS K VQ+F SIRE+EV+N IKSI S++G
Sbjct: 101  FKIVSYDFSDMVVSPYGNYWRQLRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEG 160

Query: 358  SVINLSKRIYTLSYSIIAEAAFGKGSKYTEEFIPLMEVVLKLFGGFSLVDMYPSVKILER 537
            + INLSK I  L Y I   AAFG+ +K TEEFI L++ + K     ++ DM+PS+K L+ 
Sbjct: 161  TRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQL 220

Query: 538  ITGVRNKLERTQEQLDAVLESILNEHKNR-GAESEAGNQEAKEDLVDVLLNVQKSGEFGA 714
            I+  + K+E+  +Q D ++E+IL  HK +        N E KEDLVDVLLN+Q+  +F A
Sbjct: 221  ISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKKEDLVDVLLNIQRRNDFEA 280

Query: 715  PLSDNSIKAVIYDIFSAGGDTSATTVTWAMFEMIKNPTMLERAQDEVRKVFGGRGNADES 894
            PL D +IKA+I++IFSAG +TS+TTV WAM EMIKNPT++++AQ+EVRKVF   GN DE+
Sbjct: 281  PLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDET 340

Query: 895  QLHELKYLQAVIKETLRLHAPVPLLLPRESSEQCEINGYVIPAKTRVIINCWAMGRDPMY 1074
            +LH+LKYLQAVIKETLRLH PVPLLLPRE  EQC+I GY IP+K+RVI+N WA+GRDP Y
Sbjct: 341  KLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDPNY 400

Query: 1075 WTDPER 1092
            W +PE+
Sbjct: 401  WIEPEK 406


>ref|XP_003519623.1| PREDICTED: cytochrome P450 71D10-like [Glycine max]
          Length = 508

 Score =  391 bits (1004), Expect = e-106
 Identities = 191/363 (52%), Positives = 262/363 (72%), Gaps = 1/363 (0%)
 Frame = +1

Query: 7    LGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXXMSDHDIIFANRPPLLAACV 186
            LG LPHR LA LA +YGPLMH+QLG                M  HDIIFANRP +LAA V
Sbjct: 55   LGTLPHRSLARLANQYGPLMHMQLGELSCIMVSSPEMAKEVMKTHDIIFANRPYVLAADV 114

Query: 187  FTHNCQDIAFSPYGNYWRQMRKLCATELLSSKRVQTFGSIREKEVMNMIKSISSRKGSVI 366
             T+  + + FSP G YWRQMRK+C  ELL+ KRV +F SIRE+E+   +K +S  +GS I
Sbjct: 115  ITYGSKGMTFSPQGTYWRQMRKICTMELLAPKRVDSFRSIREQELSIFVKEMSLSEGSPI 174

Query: 367  NLSKRIYTLSYSIIAEAAFGKGSKYTEEFIPLMEVVLKLFGGFSLVDMYPSVKILERITG 546
            NLS++I +L+Y +I+  AFGK SK  E +I  M+ V     GFSL D+YPS+ +L+ +TG
Sbjct: 175  NLSEKISSLAYGLISRIAFGKKSKDQEAYIEFMKGVTDTVSGFSLADLYPSIGLLQVLTG 234

Query: 547  VRNKLERTQEQLDAVLESILNEHKNRGAESE-AGNQEAKEDLVDVLLNVQKSGEFGAPLS 723
            +R ++E+ +  +D ++++I+ +H+++ ++++    +E  EDLVDVLL +QK+G    PLS
Sbjct: 235  IRPRVEKIRRGMDRIIDNIVRDHRDKNSDTQPVVGEENGEDLVDVLLRLQKNGNLQHPLS 294

Query: 724  DNSIKAVIYDIFSAGGDTSATTVTWAMFEMIKNPTMLERAQDEVRKVFGGRGNADESQLH 903
            D  +KA I DIFSAG +T++TT+ WAM E++KNP M+E+AQ EVR+VF  +G  DE+ +H
Sbjct: 295  DTVVKATIMDIFSAGSETTSTTMEWAMSELVKNPRMMEKAQIEVRRVFDPKGYVDETSIH 354

Query: 904  ELKYLQAVIKETLRLHAPVPLLLPRESSEQCEINGYVIPAKTRVIINCWAMGRDPMYWTD 1083
            ELKYL++VIKETLRLH PVPLLLPRE SE+CEINGY IPAK++VI+N WA+GRDP YW +
Sbjct: 355  ELKYLRSVIKETLRLHTPVPLLLPRECSERCEINGYEIPAKSKVIVNAWAIGRDPNYWIE 414

Query: 1084 PER 1092
             E+
Sbjct: 415  AEK 417


>ref|XP_002332458.1| cytochrome P450 [Populus trichocarpa] gi|222832529|gb|EEE71006.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 490

 Score =  389 bits (999), Expect = e-106
 Identities = 190/364 (52%), Positives = 259/364 (71%), Gaps = 1/364 (0%)
 Frame = +1

Query: 4    LLGPLPHRILADLAKKYGPLMHIQLGXXXXXXXXXXXXXXXXMSDHDIIFANRPPLLAAC 183
            L+G LPH    D+AKKYGP+MH++LG                M  HD+IFA RP  +AA 
Sbjct: 34   LVGSLPHHRFRDMAKKYGPVMHLRLGEVTHVLISSAETAKEVMKTHDLIFAQRPAPIAAK 93

Query: 184  VFTHNCQDIAFSPYGNYWRQMRKLCATELLSSKRVQTFGSIREKEVMNMIKSISSRKGSV 363
            + ++NC DIAF+PYG+YWR +RKLC  ELLS+KRV++F SIRE+EV  +++SISS   S 
Sbjct: 94   ILSYNCMDIAFAPYGDYWRMLRKLCVLELLSAKRVRSFRSIREEEVWRVVRSISSSAWSP 153

Query: 364  INLSKRIYTLSYSIIAEAAFGKGSKYTEEFIPLMEVVLKLFGGFSLVDMYPSVKILERIT 543
            +N S+ I +L+Y I + AAFGK  K  + FIP ++   K  GG+SL D+YPS+K+L  I+
Sbjct: 154  VNFSRMISSLTYCITSRAAFGKICKGEDVFIPAVKEANKAAGGYSLADLYPSIKLLSVIS 213

Query: 544  GVRNKLERTQEQLDAVLESILNEHKNRGA-ESEAGNQEAKEDLVDVLLNVQKSGEFGAPL 720
            G+R  LE+   +LD +L+ I+NEH+++    ++ G  E + DLVDVLL +Q  G+    L
Sbjct: 214  GMRLTLEKIHARLDKILQEIINEHRSKKEMAAKTGADEEEHDLVDVLLGIQDQGDTEFSL 273

Query: 721  SDNSIKAVIYDIFSAGGDTSATTVTWAMFEMIKNPTMLERAQDEVRKVFGGRGNADESQL 900
            +DN+IKA+I D+F AG DTS+TTV WAM EM+K+P ++++AQ+EVR+VFG +G  DE+ L
Sbjct: 274  TDNNIKAIILDLFVAGTDTSSTTVVWAMSEMVKHPRVMKKAQEEVRQVFGDKGTVDEAGL 333

Query: 901  HELKYLQAVIKETLRLHAPVPLLLPRESSEQCEINGYVIPAKTRVIINCWAMGRDPMYWT 1080
            HEL YL+  IKET RLH PVPLLLPRES E C+INGY IP K++VI+N  A+GRDP YW 
Sbjct: 334  HELNYLKLAIKETFRLHPPVPLLLPRESREDCKINGYDIPIKSKVIVNVSAIGRDPTYWN 393

Query: 1081 DPER 1092
            +PER
Sbjct: 394  EPER 397