BLASTX nr result
ID: Cephaelis21_contig00005059
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cephaelis21_contig00005059
(1588 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI31395.3| unnamed protein product [Vitis vinifera] 198 3e-69
ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like... 198 3e-69
ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|2... 206 2e-68
ref|XP_002532780.1| pullulanase, putative [Ricinus communis] gi|... 199 2e-67
ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like... 195 3e-63
>emb|CBI31395.3| unnamed protein product [Vitis vinifera]
Length = 956
Score = 198 bits (504), Expect(2) = 3e-69
Identities = 92/139 (66%), Positives = 106/139 (76%)
Frame = +2
Query: 2 YNPFILPSNLDVESLLKCQLAVVIFSSDGKCTTATSLQLPGVLDELFFYSGPLGAVFSSD 181
Y F +P +D +SL+KCQLAV FS DG+C T LQLPGVLDELF Y GPLGA+FS +
Sbjct: 147 YKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTGLQLPGVLDELFSYEGPLGAIFSKE 206
Query: 182 AISLYLWAPTAQSVCAFIYKDLFSDDSREII*LNELNGVWSSTGPREWEGCYYVFEVSVY 361
A++LYLWAPTAQ V A IY D D EI+ L E NGVWS++GP+ WEGCYY +EVSVY
Sbjct: 207 AVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVY 266
Query: 362 HPSTLQIEKCIVNDPYARG 418
HPSTLQIEKC NDPYARG
Sbjct: 267 HPSTLQIEKCTANDPYARG 285
Score = 92.0 bits (227), Expect(2) = 3e-69
Identities = 45/52 (86%), Positives = 47/52 (90%)
Frame = +3
Query: 480 LSADSRSTLLVNLGSDSLKPEGWDNLADEKPNLLSFADISIYELHIRDFSLS 635
LSAD+R TLLVNL SD LKPEGWDNLADEKP LLSF+DISIYELHIRDFS S
Sbjct: 286 LSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSAS 337
Score = 115 bits (288), Expect = 3e-23
Identities = 52/63 (82%), Positives = 57/63 (90%)
Frame = +2
Query: 1160 YNPVLWRVPKGSYASNPNGSCRSLEFRKMVQALNQIGLRVMLDVVYNHIHASGPLDDNSV 1339
YNPVLW VPKGSYASNPN CR+LEFRKMVQALN+IG RV+LDVVYNH+H SGP D+NSV
Sbjct: 427 YNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFDENSV 486
Query: 1340 LDK 1348
LDK
Sbjct: 487 LDK 489
>ref|XP_002271820.2| PREDICTED: pullulanase 1, chloroplastic-like [Vitis vinifera]
Length = 896
Score = 198 bits (504), Expect(2) = 3e-69
Identities = 92/139 (66%), Positives = 106/139 (76%)
Frame = +2
Query: 2 YNPFILPSNLDVESLLKCQLAVVIFSSDGKCTTATSLQLPGVLDELFFYSGPLGAVFSSD 181
Y F +P +D +SL+KCQLAV FS DG+C T LQLPGVLDELF Y GPLGA+FS +
Sbjct: 87 YKAFKVPQAVDAKSLIKCQLAVAAFSCDGQCNNVTGLQLPGVLDELFSYEGPLGAIFSKE 146
Query: 182 AISLYLWAPTAQSVCAFIYKDLFSDDSREII*LNELNGVWSSTGPREWEGCYYVFEVSVY 361
A++LYLWAPTAQ V A IY D D EI+ L E NGVWS++GP+ WEGCYY +EVSVY
Sbjct: 147 AVTLYLWAPTAQVVHACIYGDPSGGDPLEIVCLEEFNGVWSASGPKSWEGCYYEYEVSVY 206
Query: 362 HPSTLQIEKCIVNDPYARG 418
HPSTLQIEKC NDPYARG
Sbjct: 207 HPSTLQIEKCTANDPYARG 225
Score = 92.0 bits (227), Expect(2) = 3e-69
Identities = 45/52 (86%), Positives = 47/52 (90%)
Frame = +3
Query: 480 LSADSRSTLLVNLGSDSLKPEGWDNLADEKPNLLSFADISIYELHIRDFSLS 635
LSAD+R TLLVNL SD LKPEGWDNLADEKP LLSF+DISIYELHIRDFS S
Sbjct: 226 LSADARRTLLVNLDSDDLKPEGWDNLADEKPALLSFSDISIYELHIRDFSAS 277
Score = 115 bits (288), Expect = 3e-23
Identities = 52/63 (82%), Positives = 57/63 (90%)
Frame = +2
Query: 1160 YNPVLWRVPKGSYASNPNGSCRSLEFRKMVQALNQIGLRVMLDVVYNHIHASGPLDDNSV 1339
YNPVLW VPKGSYASNPN CR+LEFRKMVQALN+IG RV+LDVVYNH+H SGP D+NSV
Sbjct: 367 YNPVLWGVPKGSYASNPNSPCRTLEFRKMVQALNRIGFRVVLDVVYNHLHGSGPFDENSV 426
Query: 1340 LDK 1348
LDK
Sbjct: 427 LDK 429
>ref|XP_002315334.1| predicted protein [Populus trichocarpa] gi|222864374|gb|EEF01505.1|
predicted protein [Populus trichocarpa]
Length = 893
Score = 206 bits (523), Expect(2) = 2e-68
Identities = 94/139 (67%), Positives = 111/139 (79%)
Frame = +2
Query: 2 YNPFILPSNLDVESLLKCQLAVVIFSSDGKCTTATSLQLPGVLDELFFYSGPLGAVFSSD 181
Y F +PS +D +SL+KCQLAV F SDGKC+ AT LQLPGVLDELF Y GPLGA +S D
Sbjct: 86 YKAFKVPSTVDAKSLVKCQLAVATFGSDGKCSYATGLQLPGVLDELFAYDGPLGAHYSED 145
Query: 182 AISLYLWAPTAQSVCAFIYKDLFSDDSREII*LNELNGVWSSTGPREWEGCYYVFEVSVY 361
A+SLYLWAPTAQ+VCA +YK+ S D E++ L E+NGVWS G ++WEGCYYV+EVSVY
Sbjct: 146 AVSLYLWAPTAQAVCACVYKNANSRDPVEVVQLKEVNGVWSVEGSKDWEGCYYVYEVSVY 205
Query: 362 HPSTLQIEKCIVNDPYARG 418
HPSTL +EKC NDPYARG
Sbjct: 206 HPSTLHVEKCYANDPYARG 224
Score = 82.0 bits (201), Expect(2) = 2e-68
Identities = 38/50 (76%), Positives = 44/50 (88%)
Frame = +3
Query: 480 LSADSRSTLLVNLGSDSLKPEGWDNLADEKPNLLSFADISIYELHIRDFS 629
LS DS+ TL VNL SD+LKPEGW+ LADEKP +LSF+DISIYELH+RDFS
Sbjct: 225 LSPDSQRTLFVNLDSDTLKPEGWEKLADEKPIILSFSDISIYELHVRDFS 274
Score = 117 bits (292), Expect = 1e-23
Identities = 52/63 (82%), Positives = 59/63 (93%)
Frame = +2
Query: 1160 YNPVLWRVPKGSYASNPNGSCRSLEFRKMVQALNQIGLRVMLDVVYNHIHASGPLDDNSV 1339
YNPVLW VPKGSYASNP+GSCR++EFRKMVQALN IGLRV+LDVVYNH+H +GP D+NSV
Sbjct: 366 YNPVLWGVPKGSYASNPSGSCRTIEFRKMVQALNHIGLRVVLDVVYNHLHGNGPFDENSV 425
Query: 1340 LDK 1348
LDK
Sbjct: 426 LDK 428
>ref|XP_002532780.1| pullulanase, putative [Ricinus communis]
gi|223527468|gb|EEF29599.1| pullulanase, putative
[Ricinus communis]
Length = 964
Score = 199 bits (505), Expect(2) = 2e-67
Identities = 92/139 (66%), Positives = 109/139 (78%)
Frame = +2
Query: 2 YNPFILPSNLDVESLLKCQLAVVIFSSDGKCTTATSLQLPGVLDELFFYSGPLGAVFSSD 181
Y F P LD +SLLKCQLAV + +DGKC +AT LQLPG+LDEL+ Y GPLGA +S +
Sbjct: 157 YRAFKAPPTLDAKSLLKCQLAVASYEADGKCRSATGLQLPGILDELYSYDGPLGAHYSKN 216
Query: 182 AISLYLWAPTAQSVCAFIYKDLFSDDSREII*LNELNGVWSSTGPREWEGCYYVFEVSVY 361
A+SL+LWAPTAQ+V +IYKD FS EI L E+NGVWS GP++WEGCYYV+EVSVY
Sbjct: 217 AVSLHLWAPTAQAVLVYIYKDSFSKVPLEIHQLKEVNGVWSIKGPKDWEGCYYVYEVSVY 276
Query: 362 HPSTLQIEKCIVNDPYARG 418
HPSTL+IEKC NDPYARG
Sbjct: 277 HPSTLRIEKCYANDPYARG 295
Score = 85.1 bits (209), Expect(2) = 2e-67
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = +3
Query: 480 LSADSRSTLLVNLGSDSLKPEGWDNLADEKPNLLSFADISIYELHIRDFS 629
LS+D + TLLVNL S+SLKPEGWDNLA EKP+LLSF DIS+YELHIRDFS
Sbjct: 296 LSSDGKRTLLVNLTSESLKPEGWDNLAKEKPSLLSFCDISLYELHIRDFS 345
Score = 113 bits (283), Expect = 1e-22
Identities = 51/63 (80%), Positives = 58/63 (92%)
Frame = +2
Query: 1160 YNPVLWRVPKGSYASNPNGSCRSLEFRKMVQALNQIGLRVMLDVVYNHIHASGPLDDNSV 1339
YNPVLW VPKGSYASNPNG R+++FRKMVQALN+IGLRV+LDVVYNH+H SGP D+NSV
Sbjct: 437 YNPVLWGVPKGSYASNPNGPSRTIQFRKMVQALNRIGLRVVLDVVYNHLHGSGPFDENSV 496
Query: 1340 LDK 1348
LDK
Sbjct: 497 LDK 499
>ref|XP_003536283.1| PREDICTED: pullulanase 1, chloroplastic-like [Glycine max]
Length = 951
Score = 195 bits (496), Expect(2) = 3e-63
Identities = 92/136 (67%), Positives = 108/136 (79%)
Frame = +2
Query: 11 FILPSNLDVESLLKCQLAVVIFSSDGKCTTATSLQLPGVLDELFFYSGPLGAVFSSDAIS 190
F LP LDV+ LLK +LAVVI SDG+C T LQLPGVLD+LF YSGPLGA+FS +A+S
Sbjct: 145 FSLPPTLDVKPLLKFRLAVVICDSDGECMNCTGLQLPGVLDDLFSYSGPLGALFSEEAVS 204
Query: 191 LYLWAPTAQSVCAFIYKDLFSDDSREII*LNELNGVWSSTGPREWEGCYYVFEVSVYHPS 370
LYLWAPTAQ+V A+IYKD DD EI+ L E NGVW + GP+ WEGCYYV+EV VYHPS
Sbjct: 205 LYLWAPTAQAVHAYIYKDPSGDDPIEIVCLEEENGVWRTKGPKSWEGCYYVYEVCVYHPS 264
Query: 371 TLQIEKCIVNDPYARG 418
T++IEKC +DPYARG
Sbjct: 265 TMRIEKCYTSDPYARG 280
Score = 74.7 bits (182), Expect(2) = 3e-63
Identities = 34/52 (65%), Positives = 43/52 (82%)
Frame = +3
Query: 480 LSADSRSTLLVNLGSDSLKPEGWDNLADEKPNLLSFADISIYELHIRDFSLS 635
LS+D R + L+NL S LKP+GWDNLA++KP + SF+DISIYE+HIRDFS S
Sbjct: 281 LSSDGRRSFLLNLDSVKLKPDGWDNLANKKPTIHSFSDISIYEMHIRDFSAS 332
Score = 107 bits (266), Expect = 1e-20
Identities = 49/63 (77%), Positives = 55/63 (87%)
Frame = +2
Query: 1160 YNPVLWRVPKGSYASNPNGSCRSLEFRKMVQALNQIGLRVMLDVVYNHIHASGPLDDNSV 1339
YNPVLW VPKGSYASNPNG R++EFRKMV ALN IGLRV+LDVVYNH+ SGP D++SV
Sbjct: 422 YNPVLWGVPKGSYASNPNGPYRTIEFRKMVMALNHIGLRVVLDVVYNHLQGSGPFDEHSV 481
Query: 1340 LDK 1348
LDK
Sbjct: 482 LDK 484