BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Catharanthus22_contig00022261
         (346 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEX07771.1| cytochrome P450 [Catharanthus roseus]                  100   3e-19
gb|AAO32822.1| cytochrome P450 71D1 [Catharanthus roseus]              70   3e-10
gb|ACD42776.1| cytochrome P450 71D1 [Catharanthus roseus]              70   3e-10
gb|AGN04217.1| cytochrome P450 [Salvia miltiorrhiza]                   69   6e-10
ref|XP_006339043.1| PREDICTED: premnaspirodiene oxygenase-like [...    66   5e-09
ref|XP_004249812.1| PREDICTED: premnaspirodiene oxygenase-like [...    64   2e-08
dbj|BAN58140.1| putative premaspirodiene oxygenase [Hyoscyamus a...    64   3e-08
gb|AAO32823.1| cytochrome P450 71D2 [Catharanthus roseus]              64   3e-08
sp|A6YIH8.1|C7D55_HYOMU RecName: Full=Premnaspirodiene oxygenase...    64   3e-08
ref|XP_006350395.1| PREDICTED: premnaspirodiene oxygenase-like [...    63   4e-08
ref|XP_006339046.1| PREDICTED: premnaspirodiene oxygenase-like [...    63   4e-08
dbj|BAJ39894.1| P450 mono-oxygenase [Zingiber zerumbet]                62   6e-08
ref|XP_006341719.1| PREDICTED: premnaspirodiene oxygenase-like [...    62   1e-07
gb|EOY04049.1| Cytochrome P450 71D10, putative [Theobroma cacao]       62   1e-07
ref|XP_004237369.1| PREDICTED: premnaspirodiene oxygenase-like [...    61   1e-07
gb|ABC69404.1| CYP71D51v2 [Nicotiana tabacum] gi|482680203|gb|AG...    61   1e-07
gb|ABC69405.1| CYP71D51v3 [Nicotiana tabacum]                          61   1e-07
emb|CAC24711.1| cytochrome P450 [Solanum tuberosum]                    61   1e-07
gb|ABQ24001.1| limonene hydroxylase [Mentha arvensis]                  61   2e-07
ref|XP_006350396.1| PREDICTED: premnaspirodiene oxygenase-like [...    60   2e-07

>gb|AEX07771.1| cytochrome P450 [Catharanthus roseus]
          Length = 506

 Score =  100 bits (248), Expect = 3e-19
 Identities = 55/115 (47%), Positives = 78/115 (67%), Gaps = 3/115 (2%)
 Frame = -3

Query: 344 SKLISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSY---NVLEVTE 174
           SKL+++CT+SI+N+ AFG+VR+E E F++LI Q  ALAGGFKLVDLFPSY   +VLE TE
Sbjct: 176 SKLMTSCTNSIINKVAFGKVRYEREVFIDLINQILALAGGFKLVDLFPSYKILHVLEGTE 235

Query: 173 QKL*IFATRLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEI 9
           +KL     ++ K   K      +      +     GQE+I+D+L+RIE+ GDL++
Sbjct: 236 RKLWEIRGKIDKILDKVIDEHRENSSRTGKGNGCNGQEDIVDILLRIEEGGDLDL 290


>gb|AAO32822.1| cytochrome P450 71D1 [Catharanthus roseus]
          Length = 495

 Score = 70.1 bits (170), Expect = 3e-10
 Identities = 43/120 (35%), Positives = 73/120 (60%), Gaps = 7/120 (5%)
 Frame = -3

Query: 344 SKLISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL 165
           S+ I++ T+S+V RAAFG+V      F++L+K+A  +AGGF   D+FPSY  L V     
Sbjct: 166 SEKINSYTTSMVARAAFGKVNDAGVTFLKLVKEASEVAGGFDPADIFPSYKFLNVFFNS- 224

Query: 164 *IFATRLIKFWTKS*LSTE-------KTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
               ++L+K   K+ +  E       K ++  ++A  + G+E++ID+L+ I+DSG+L+ P
Sbjct: 225 ---RSKLLKIHGKTDMILEEMIDEHIKNHEMGKKANGENGKEDVIDILLSIKDSGELQFP 281


>gb|ACD42776.1| cytochrome P450 71D1 [Catharanthus roseus]
          Length = 507

 Score = 70.1 bits (170), Expect = 3e-10
 Identities = 43/120 (35%), Positives = 73/120 (60%), Gaps = 7/120 (5%)
 Frame = -3

Query: 344 SKLISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL 165
           S+ I++ T+S+V RAAFG+V      F++L+K+A  +AGGF   D+FPSY  L V     
Sbjct: 178 SEKINSYTTSMVARAAFGKVNDAGVTFLKLVKEASEVAGGFDPADIFPSYKFLNVFFNS- 236

Query: 164 *IFATRLIKFWTKS*LSTE-------KTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
               ++L+K   K+ +  E       K ++  ++A  + G+E++ID+L+ I+DSG+L+ P
Sbjct: 237 ---RSKLLKIHGKTDMILEEMIDEHIKNHEMGKKANGENGKEDVIDILLSIKDSGELQFP 293


>gb|AGN04217.1| cytochrome P450 [Salvia miltiorrhiza]
          Length = 502

 Score = 68.9 bits (167), Expect = 6e-10
 Identities = 43/111 (38%), Positives = 66/111 (59%), Gaps = 1/111 (0%)
 Frame = -3

Query: 335 ISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL*IF 156
           I T TS+I  RAAFG+V  + +G + L K+A A+AGGF+L DLFPS+ +L V        
Sbjct: 181 IFTFTSTITCRAAFGKVMTDRDGLIALFKEAVAMAGGFELADLFPSWKLLNVLSWS---- 236

Query: 155 ATRLIKFWTKS*LSTEKTYQELE-RAALKLGQENIIDVLIRIEDSGDLEIP 6
             RL +   K     +    E + + + + G E+I+DVLIR++ +G+L+ P
Sbjct: 237 KYRLWRMRGKLDAILDGIIDEHKLKQSGEFGGEDIVDVLIRMQQTGELKFP 287


>ref|XP_006339043.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum tuberosum]
          Length = 509

 Score = 65.9 bits (159), Expect = 5e-09
 Identities = 38/112 (33%), Positives = 62/112 (55%), Gaps = 7/112 (6%)
 Frame = -3

Query: 320 SSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL*IFATRLI 141
           SSI  +AAFGE+  + E F++++K+   LAGGF L D+FPS  +L+V         +R++
Sbjct: 186 SSITCKAAFGELLKDQEKFIQMVKEVMELAGGFNLADIFPSIKILQVLSG----VRSRIL 241

Query: 140 KFWTKS*LSTEKTYQELERAALK-------LGQENIIDVLIRIEDSGDLEIP 6
           K         E    E ++            G E+++DVL+R+ +SG+L+IP
Sbjct: 242 KVHKNVDAIVEDVINEHKKNIASGKKGNGAFGGEDLVDVLLRLMESGELKIP 293


>ref|XP_004249812.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum lycopersicum]
          Length = 511

 Score = 63.9 bits (154), Expect = 2e-08
 Identities = 38/117 (32%), Positives = 64/117 (54%), Gaps = 7/117 (5%)
 Frame = -3

Query: 335 ISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL*IF 156
           IS   SSI  + AFGE+  + E F+ ++++   +AGGF   D+FPS  +L+V        
Sbjct: 181 ISWYQSSITCKTAFGELLKDQEKFLGILREFTEVAGGFSTADIFPSIKILQVLSG----L 236

Query: 155 ATRLIKFWTKS*LSTEKTYQELERAALK-------LGQENIIDVLIRIEDSGDLEIP 6
            +R++KF     +  E    E ++            G EN++DVL+R+++SG+L+IP
Sbjct: 237 KSRILKFHKNVDVIVEDVINEHKKNLASGKKGNGAFGGENLVDVLLRLKESGELKIP 293


>dbj|BAN58140.1| putative premaspirodiene oxygenase [Hyoscyamus albus]
          Length = 502

 Score = 63.5 bits (153), Expect = 3e-08
 Identities = 40/109 (36%), Positives = 62/109 (56%), Gaps = 3/109 (2%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEV---TEQKL*IFA 153
           TSS+  R+AFG+V  E E F++LIK+   LAGGF + D+FPS   L V    E K+ + A
Sbjct: 178 TSSMTCRSAFGKVFKEQETFIQLIKEVIGLAGGFDVADIFPSLKFLHVLTGMEGKI-MKA 236

Query: 152 TRLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
              +    +  ++  K    + +    LG E++IDVL+R+ + G L+ P
Sbjct: 237 HHKVDAIVEDVINEHKKNLAMGKTNGALGGEDLIDVLLRLMNDGGLQFP 285


>gb|AAO32823.1| cytochrome P450 71D2 [Catharanthus roseus]
          Length = 430

 Score = 63.5 bits (153), Expect = 3e-08
 Identities = 43/119 (36%), Positives = 68/119 (57%), Gaps = 7/119 (5%)
 Frame = -3

Query: 344 SKLISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL 165
           S+ I   T+S+V RAAFG+V      F++L+ +A  +A GF   D+FPSY  L V     
Sbjct: 101 SEKIKLYTTSMVARAAFGKVNDAGVTFLKLVTEAAEVAEGFDPADMFPSYKFLNVFFNS- 159

Query: 164 *IFATRLIKFWTKS*LSTE-------KTYQELERAALKLGQENIIDVLIRIEDSGDLEI 9
               + L+K   K+ +  E       K++Q  ++A  + G+E++ID+L+ I+DSGDL I
Sbjct: 160 ---RSNLLKIHGKTDMILEEMIDEHIKSHQMGKKANGENGEEDVIDILLSIKDSGDLGI 215


>sp|A6YIH8.1|C7D55_HYOMU RecName: Full=Premnaspirodiene oxygenase; Short=HPO; AltName:
           Full=Cytochrome P450 71D55 gi|151335776|gb|ABS00393.1|
           cytochrome P450 hydroxylase [Hyoscyamus muticus]
          Length = 502

 Score = 63.5 bits (153), Expect = 3e-08
 Identities = 40/109 (36%), Positives = 62/109 (56%), Gaps = 3/109 (2%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEV---TEQKL*IFA 153
           TSS+  R+AFG+V  E E F++LIK+   LAGGF + D+FPS   L V    E K+ + A
Sbjct: 178 TSSMTCRSAFGKVFKEQETFIQLIKEVIGLAGGFDVADIFPSLKFLHVLTGMEGKI-MKA 236

Query: 152 TRLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
              +    +  ++  K    + +    LG E++IDVL+R+ + G L+ P
Sbjct: 237 HHKVDAIVEDVINEHKKNLAMGKTNGALGGEDLIDVLLRLMNDGGLQFP 285


>ref|XP_006350395.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum tuberosum]
          Length = 502

 Score = 63.2 bits (152), Expect = 4e-08
 Identities = 40/108 (37%), Positives = 61/108 (56%), Gaps = 2/108 (1%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE--QKL*IFAT 150
           TSS+  R+AFG+V  E + F++LIK+   LAGGF + DLFPS  +L V    +   + A 
Sbjct: 178 TSSMTCRSAFGKVFKEQDKFIQLIKEVIGLAGGFDVADLFPSLKLLHVLSGMKGKIMNAH 237

Query: 149 RLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
             I    +  ++  K    + +    LG E++IDVLIR+ + G L+ P
Sbjct: 238 HKIDAIVEDVINEHKKNIAMGKTNGALGGEDLIDVLIRLMNDGGLQFP 285


>ref|XP_006339046.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum tuberosum]
          Length = 510

 Score = 63.2 bits (152), Expect = 4e-08
 Identities = 35/112 (31%), Positives = 63/112 (56%), Gaps = 7/112 (6%)
 Frame = -3

Query: 320 SSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL*IFATRLI 141
           SSI  + AFGE+  + E F+ ++++   +AGGF L D+FPS  +L+V         ++++
Sbjct: 186 SSITCKTAFGELLKDQEKFLGILRELMEVAGGFSLADIFPSIKILQVLSG----LKSKIL 241

Query: 140 KFWTKS*LSTEKTYQELERAALK-------LGQENIIDVLIRIEDSGDLEIP 6
           K    + +  E    E ++            G EN++DVL+R+++SG+L+IP
Sbjct: 242 KVHKNADVIVEDVINEHKKNIASGKKGNGAFGGENLVDVLLRLKESGELKIP 293


>dbj|BAJ39894.1| P450 mono-oxygenase [Zingiber zerumbet]
          Length = 505

 Score = 62.4 bits (150), Expect = 6e-08
 Identities = 41/113 (36%), Positives = 69/113 (61%), Gaps = 3/113 (2%)
 Frame = -3

Query: 335 ISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL*IF 156
           ++  T+SIV +A+FG    + + F+E IK+   +A GF + DLFPS  V++V    L  F
Sbjct: 179 LNELTNSIVVQASFGRRCQQQKKFMETIKEVIKMASGFSVGDLFPSLQVVDV----LTGF 234

Query: 155 ATRLIKFWTKS*LSTEKTYQELERAALKLG---QENIIDVLIRIEDSGDLEIP 6
           +T+L K+  K     + T +E +    + G   +E++IDVL+R++D G+LE+P
Sbjct: 235 STQLKKYHKKLDAILDATIKEHQMT--RDGGEEEEDLIDVLLRLKDEGNLEVP 285


>ref|XP_006341719.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum tuberosum]
          Length = 506

 Score = 61.6 bits (148), Expect = 1e-07
 Identities = 43/119 (36%), Positives = 66/119 (55%), Gaps = 6/119 (5%)
 Frame = -3

Query: 344 SKLISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLE-VTEQK 168
           S++I T T++I+ RAAFG      + F+  +++   LAGGF L D FPS   L  +T  K
Sbjct: 176 SQMIFTLTNNIIARAAFGRKLENQDEFIATLRKIVDLAGGFDLPDTFPSLKFLHPLTGAK 235

Query: 167 L*IFATRLIKFWTKS*LSTEKTYQELERAALKLG-----QENIIDVLIRIEDSGDLEIP 6
                  + K   +     E   QE  +AA KL      +E+++DVL+R+++SGDLE+P
Sbjct: 236 -----AAMEKIHHQIDKILESVLQE-HKAARKLAINREDKEDLVDVLLRVQESGDLEVP 288


>gb|EOY04049.1| Cytochrome P450 71D10, putative [Theobroma cacao]
          Length = 509

 Score = 61.6 bits (148), Expect = 1e-07
 Identities = 38/116 (32%), Positives = 67/116 (57%), Gaps = 6/116 (5%)
 Frame = -3

Query: 335 ISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE------ 174
           I + T  I +RAAFG+   + E F+ ++ +   LAGGF L D++PS  +L++        
Sbjct: 179 IFSMTYGITSRAAFGKKSKDQEEFIRIMMETIKLAGGFCLADMYPSNELLKLISGVRLKL 238

Query: 173 QKL*IFATRLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
           +KL + + R+++         EKT +  E    + G+E+++DVL++++ S DLEIP
Sbjct: 239 EKLQLASDRILEDIVNE--HKEKTNRTSE-TGNQQGEEDLLDVLLKLQQSSDLEIP 291


>ref|XP_004237369.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum lycopersicum]
          Length = 501

 Score = 61.2 bits (147), Expect = 1e-07
 Identities = 37/108 (34%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE--QKL*IFAT 150
           TSS+  R+AFG+V  E + F++LIK+   LAGGF + D+FPS   L V    +   + A 
Sbjct: 180 TSSMTCRSAFGKVFKEQDKFIQLIKEVIGLAGGFDVADIFPSLKFLHVLSGMKGKIVNAH 239

Query: 149 RLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
             +    +  ++  K    + +    LG E++IDVL+R+ + G L+ P
Sbjct: 240 HKVDAIVEDVINEHKKNIAMGKTNGALGGEDLIDVLLRLMNDGGLQFP 287


>gb|ABC69404.1| CYP71D51v2 [Nicotiana tabacum] gi|482680203|gb|AGK30193.1|
           cytochrome P450 CYP71D51 [Nicotiana tabacum]
          Length = 514

 Score = 61.2 bits (147), Expect = 1e-07
 Identities = 37/108 (34%), Positives = 61/108 (56%), Gaps = 2/108 (1%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE--QKL*IFAT 150
           TSS+  R+AFG+V  E + F++LIK+   LAGGF + D+FPS   L V    +   + A 
Sbjct: 190 TSSMTCRSAFGQVFKEQDKFIQLIKEVILLAGGFDVADIFPSLKFLHVLSGMKGKIMNAH 249

Query: 149 RLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
             +    ++ ++  K    + +    LG E++IDVL+R+ + G L+ P
Sbjct: 250 HKVDAIVENVINEHKKNLAIGKTNGALGGEDLIDVLLRLMNDGGLQFP 297


>gb|ABC69405.1| CYP71D51v3 [Nicotiana tabacum]
          Length = 514

 Score = 61.2 bits (147), Expect = 1e-07
 Identities = 37/108 (34%), Positives = 61/108 (56%), Gaps = 2/108 (1%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE--QKL*IFAT 150
           TSS+  R+AFG+V  E + F++LIK+   LAGGF + D+FPS   L V    +   + A 
Sbjct: 190 TSSMTCRSAFGQVFKEQDKFIQLIKEVILLAGGFDVADIFPSLKFLHVLSGMKGKIMNAH 249

Query: 149 RLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
             +    ++ ++  K    + +    LG E++IDVL+R+ + G L+ P
Sbjct: 250 HKVDAIVENVINEHKKNLAIGKTNGALGGEDLIDVLLRLMNDGGLQFP 297


>emb|CAC24711.1| cytochrome P450 [Solanum tuberosum]
          Length = 502

 Score = 61.2 bits (147), Expect = 1e-07
 Identities = 38/108 (35%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE--QKL*IFAT 150
           TSS+  R+AFG+V  E + F++LIK+   LAGGF + D+FPS   L V    +   + A 
Sbjct: 178 TSSMTCRSAFGKVFKEQDKFIQLIKEVIGLAGGFDVADIFPSLKFLHVLSGMKGKIMNAH 237

Query: 149 RLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
             +    +  ++  K    + +    LG E++IDVLIR+ + G L+ P
Sbjct: 238 HKVDAIVEDVINEHKKKFAIGKTNGALGGEDLIDVLIRLMNDGGLQFP 285


>gb|ABQ24001.1| limonene hydroxylase [Mentha arvensis]
          Length = 500

 Score = 60.8 bits (146), Expect = 2e-07
 Identities = 40/115 (34%), Positives = 61/115 (53%), Gaps = 5/115 (4%)
 Frame = -3

Query: 335 ISTCTSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTEQKL*IF 156
           I+T T SI+ RAAFG V  + E  VEL+K A ++A GF+L DLFPS  +L        + 
Sbjct: 179 IATLTCSIICRAAFGSVIRDHEELVELVKDALSMASGFELADLFPSSKLLN-------LL 231

Query: 155 ATRLIKFW-----TKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
                K W       + L       +L+++  + G E+IIDVL R++    +++P
Sbjct: 232 CWNKSKLWRMRRRVDTILEAIVEEHKLKKSG-EFGGEDIIDVLFRMQKDSQIKVP 285


>ref|XP_006350396.1| PREDICTED: premnaspirodiene oxygenase-like [Solanum tuberosum]
          Length = 502

 Score = 60.5 bits (145), Expect = 2e-07
 Identities = 38/108 (35%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
 Frame = -3

Query: 323 TSSIVNRAAFGEVRFEPEGFVELIKQAFALAGGFKLVDLFPSYNVLEVTE--QKL*IFAT 150
           TSS+  R+AFG+V  E + F++LIK+   LAGGF + D+FPS   L V    +   + A 
Sbjct: 178 TSSMTCRSAFGKVFKEQDKFIQLIKEVIGLAGGFDVADIFPSLKFLHVLSGMKGKIMNAH 237

Query: 149 RLIKFWTKS*LSTEKTYQELERAALKLGQENIIDVLIRIEDSGDLEIP 6
             +    +  ++  K    + +    LG E++IDVLIR+ + G L+ P
Sbjct: 238 DKVDAIVEDVINEHKKNIAMGKTNGALGGEDLIDVLIRLMNDGGLQFP 285