BLASTX nr result
ID: Catharanthus22_contig00016163
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Catharanthus22_contig00016163
(1274 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 279 2e-72
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 279 2e-72
ref|XP_006340578.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 278 3e-72
ref|XP_004240740.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 276 1e-71
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 276 2e-71
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 275 2e-71
ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 275 4e-71
ref|XP_002276320.2| PREDICTED: probable (S)-N-methylcoclaurine 3... 275 4e-71
emb|CBI34742.3| unnamed protein product [Vitis vinifera] 275 4e-71
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 274 5e-71
ref|XP_006340577.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 274 6e-71
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 273 8e-71
ref|XP_002318835.2| hypothetical protein POPTR_0012s13630g [Popu... 273 1e-70
ref|XP_002318834.2| hypothetical protein POPTR_0012s13620g [Popu... 271 3e-70
ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 271 4e-70
ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 271 5e-70
emb|CAN70170.1| hypothetical protein VITISV_006874 [Vitis vinifera] 271 5e-70
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 270 7e-70
gb|ABD39696.1| littorine mutase/monooxygenase [Hyoscyamus niger] 270 9e-70
ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 269 2e-69
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 279 bits (713), Expect = 2e-72
Identities = 153/372 (41%), Positives = 227/372 (61%), Gaps = 4/372 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ L K PH+ +A LA+ GPL SL++G++ ++AS+P +A E+ K HD+ LSGR
Sbjct: 1092 IIGNLLQLG-KNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGR 1150
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ ++A A EC W+ LRTI TE+FS +A+E E R +KV E++
Sbjct: 1151 YVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELV 1210
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIVECGA-PGL 702
+FL +K GE++K+ D +F ++ NILS S D+ + E ++ + E GA P L
Sbjct: 1211 EFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDF--EREGLKDFIYRAAELGATPNL 1268
Query: 703 FDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALIKNS 879
D YP L G + KK++ + W L ER+ KQ+ +D L+A ++
Sbjct: 1269 SDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLEIR 1326
Query: 880 WDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIVLNE 1056
++D++I + E F ++ T IEWA+T+L+RN + KL+DELTK I E + E
Sbjct: 1327 FEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKII---GESPVRE 1383
Query: 1057 ECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGRDSM 1236
L LPYLQAC+KET RLHPP+P L+P A++TC+VM Y IPK++ V +N +A+GRD
Sbjct: 1384 SHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPK 1443
Query: 1237 TWDDPLSFKPER 1272
WDDPLSF PER
Sbjct: 1444 VWDDPLSFTPER 1455
Score = 276 bits (705), Expect = 2e-71
Identities = 152/377 (40%), Positives = 228/377 (60%), Gaps = 9/377 (2%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + K PH +A LA+ GPL SL++G++ ++AS+P +A E+ K HD+ LSGR
Sbjct: 587 IIGNLLQMG-KNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGR 645
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ +I A +C+ W+ LR I ELFS +A+E E R +KV E++
Sbjct: 646 YLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELV 705
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSE---EMQRIVRWIVECGA- 693
+FL +K GE++K+ D +F ++ NILS S D+ ++ ++ ++ E GA
Sbjct: 706 EFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGAT 765
Query: 694 PGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALI 870
P L D YP L G D I ++A + E W DIL ER+ + + +DFL+AL+
Sbjct: 766 PNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSH--RDFLEALL 823
Query: 871 KNSWDDNEIFTFLSEFF---VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAE 1041
+ ++D++I + E F T SLT +EWAM EL+RNQ A+ KL+ EL + + E
Sbjct: 824 EIGFEDDQINQVILELFSAGAETSSLT--VEWAMAELIRNQDAMDKLRGELRQIV---GE 878
Query: 1042 IVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYAL 1221
+ E L +LPYLQAC+KE RLHPP+P L+P A +TC+VM Y IPK++ + +N +A+
Sbjct: 879 SPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAM 938
Query: 1222 GRDSMTWDDPLSFKPER 1272
RD WDDPLSFKPER
Sbjct: 939 ARDPKIWDDPLSFKPER 955
Score = 271 bits (692), Expect = 5e-70
Identities = 153/380 (40%), Positives = 225/380 (59%), Gaps = 12/380 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + H+ +A LA+ GPL SL++G++ ++AS+ +A E+ K HD+ LSGR
Sbjct: 47 IIGNLLQMGGNL-HVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGR 105
Query: 349 YIPQINRI-LPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ A+A A C+ WR L+ I ELFS +A+E E R +KV E++
Sbjct: 106 YVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELV 165
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIV-------- 681
+FL K GE++K+ D ++ +V NILS S D S+ R VR ++
Sbjct: 166 EFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDF------SDFEGRDVRGVLLKDLLNEN 219
Query: 682 -ECGAPGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDF 855
E GA + D YP L G D I+K+ + W DIL ER+ KQ+ DF
Sbjct: 220 AELGATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDF 277
Query: 856 LDALIKNSWDDNEIFTFLSE-FFVSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDH 1032
LDAL++ ++D++I + E FF E+ + +EWAM EL++NQ A+ KL +ELT+ I
Sbjct: 278 LDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQII-- 335
Query: 1033 KAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNA 1212
E + E L LPYLQAC+KET RLHP P L+P A +TC++M Y IPK++++ +N
Sbjct: 336 -GESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNM 394
Query: 1213 YALGRDSMTWDDPLSFKPER 1272
+A+GRD TW+DPLSFKPER
Sbjct: 395 WAMGRDPGTWEDPLSFKPER 414
>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Vitis vinifera]
Length = 515
Score = 279 bits (713), Expect = 2e-72
Identities = 153/372 (41%), Positives = 227/372 (61%), Gaps = 4/372 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ L K PH+ +A LA+ GPL SL++G++ ++AS+P +A E+ K HD+ LSGR
Sbjct: 47 IIGNLLQLG-KNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGR 105
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ ++A A EC W+ LRTI TE+FS +A+E E R +KV E++
Sbjct: 106 YVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELV 165
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIVECGA-PGL 702
+FL +K GE++K+ D +F ++ NILS S D+ + E ++ + E GA P L
Sbjct: 166 EFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDF--EREGLKDFIYRAAELGATPNL 223
Query: 703 FDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALIKNS 879
D YP L G + KK++ + W L ER+ KQ+ +D L+A ++
Sbjct: 224 SDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERR--KQKNPGSSHRDLLEAFLEIR 281
Query: 880 WDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIVLNE 1056
++D++I + E F ++ T IEWA+T+L+RN + KL+DELTK I E + E
Sbjct: 282 FEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKII---GESPVRE 338
Query: 1057 ECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGRDSM 1236
L LPYLQAC+KET RLHPP+P L+P A++TC+VM Y IPK++ V +N +A+GRD
Sbjct: 339 SHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPK 398
Query: 1237 TWDDPLSFKPER 1272
WDDPLSF PER
Sbjct: 399 VWDDPLSFTPER 410
>ref|XP_006340578.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 531
Score = 278 bits (711), Expect = 3e-72
Identities = 158/377 (41%), Positives = 228/377 (60%), Gaps = 10/377 (2%)
Frame = +1
Query: 172 VGNIFHLDPKKPHISIAKLAESFGP-LFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
+GN+F + K+PH S+AKLA+S GP L S++ G+R ++AS+P +A E+ K HD+ LSGR
Sbjct: 77 IGNLFQVGRKRPHASLAKLAQSHGPDLMSIRFGTRLVVVASSPAAAAEVLKTHDRMLSGR 136
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
++ R+ + + A ECDE W+ +RTI LFS++ALE R K+ EM+
Sbjct: 137 FVSHPIRVEGSKLHNVSTAFLEECDENWKNVRTIYRGALFSNKALESQVSIREMKIWEMM 196
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIV--KSWNDSEEMQRIVRWIVECGAP- 696
+L++K G++IKIK +F + N+L +++S D++ + + +R E G
Sbjct: 197 QYLDTKKGQVIKIKFVVFVTALNVLGNLLLSVDLIDYEGKGIGARLSEYLRKFTEAGGIL 256
Query: 697 GLFDLYPALSGF--DFW--IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDA 864
L DLYP L F DF KK F I +WGDI+ + K+++ ++ DF+DA
Sbjct: 257 ELSDLYPVLGIFCTDFQGTYKKLMGIFDTICA-VWGDIVQD----KRKRDSLDAVDFVDA 311
Query: 865 LIKNSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAE 1041
L+KN + D + L E F TES T EW + EL+RN +L KL+DE+++ +
Sbjct: 312 LVKNGFTDKHVNALLVEIFGAGTESTTATSEWMLVELLRNPQSLQKLRDEISQVVGGGKG 371
Query: 1042 IVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYAL 1221
I+ E L LPYL AC KET RLHPP P L+P A+QTCEVM YRIP+NT V++N +A+
Sbjct: 372 II-KESDLPSLPYLDACFKETLRLHPPGPLLLPHRAVQTCEVMGYRIPRNTQVLVNMWAI 430
Query: 1222 GRDSMTWDDPLSFKPER 1272
RDS WDDP SFKPER
Sbjct: 431 ARDSKIWDDPSSFKPER 447
>ref|XP_004240740.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Solanum lycopersicum]
Length = 498
Score = 276 bits (707), Expect = 1e-71
Identities = 156/377 (41%), Positives = 226/377 (59%), Gaps = 10/377 (2%)
Frame = +1
Query: 172 VGNIFHLDPKKPHISIAKLAESFGP-LFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
+GN+FH+ K+PH S+AKLA+S P L SL+ G+R ++AS+P +A + K HD+ LSGR
Sbjct: 41 IGNLFHVGRKRPHASLAKLAQSHAPDLMSLRFGTRLVVVASSPAAAAAVLKTHDRLLSGR 100
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
++ R+ + + A ECDE W+ +RTI LFS++ALE R K+ EM+
Sbjct: 101 FVSHPIRVEGSKLHNVSTAFLEECDENWKNVRTIYRGALFSNKALESQVSLRENKIREMM 160
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIV--KSWNDSEEMQRIVRWIVECGAP- 696
+L++K G++IKIK +F + N+L+ +++S D++ + + +R E G
Sbjct: 161 QYLDTKMGQVIKIKFVVFVTALNVLANLLLSVDLIDFEGKGIGAGLTEYLRKFTEAGGIL 220
Query: 697 GLFDLYPAL----SGFDFWIKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDA 864
L DLYP L + KK F I +WGDI+ + KR + + ++ DF+DA
Sbjct: 221 ELSDLYPVLGILCTDLQGTYKKLIGMFDTICA-VWGDIVQD-KRKRDSQPSLDSVDFVDA 278
Query: 865 LIKNSWDDNEIFTFLSEFFVS-TESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAE 1041
L+KN + D + L E F + TES T EW + EL+R AL KL+DE+++ +
Sbjct: 279 LVKNGFTDKHVNALLLEIFAAGTESTTATSEWMLVELLRKPQALQKLRDEISQVVGGSKG 338
Query: 1042 IVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYAL 1221
I+ E L LPYL AC KET RLHPP P L+P A+QTCEVM YRIP+NT V++N +A+
Sbjct: 339 II-KESDLPSLPYLDACFKETLRLHPPGPLLLPHRAVQTCEVMGYRIPRNTQVLVNMWAI 397
Query: 1222 GRDSMTWDDPLSFKPER 1272
RDS WDDP SFKPER
Sbjct: 398 ARDSKIWDDPSSFKPER 414
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 276 bits (705), Expect = 2e-71
Identities = 152/377 (40%), Positives = 228/377 (60%), Gaps = 9/377 (2%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + K PH +A LA+ GPL SL++G++ ++AS+P +A E+ K HD+ LSGR
Sbjct: 47 IIGNLLQMG-KNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGR 105
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ +I A +C+ W+ LR I ELFS +A+E E R +KV E++
Sbjct: 106 YLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELV 165
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSE---EMQRIVRWIVECGA- 693
+FL +K GE++K+ D +F ++ NILS S D+ ++ ++ ++ E GA
Sbjct: 166 EFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGAT 225
Query: 694 PGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALI 870
P L D YP L G D I ++A + E W DIL ER+ + + +DFL+AL+
Sbjct: 226 PNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSH--RDFLEALL 283
Query: 871 KNSWDDNEIFTFLSEFF---VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAE 1041
+ ++D++I + E F T SLT +EWAM EL+RNQ A+ KL+ EL + + E
Sbjct: 284 EIGFEDDQINQVILELFSAGAETSSLT--VEWAMAELIRNQDAMDKLRGELRQIV---GE 338
Query: 1042 IVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYAL 1221
+ E L +LPYLQAC+KE RLHPP+P L+P A +TC+VM Y IPK++ + +N +A+
Sbjct: 339 SPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAM 398
Query: 1222 GRDSMTWDDPLSFKPER 1272
RD WDDPLSFKPER
Sbjct: 399 ARDPKIWDDPLSFKPER 415
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 275 bits (704), Expect = 2e-71
Identities = 152/377 (40%), Positives = 228/377 (60%), Gaps = 9/377 (2%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + K PH +A LA+ GPL SL++G++ ++AS+P +A E+ K HD+ LSGR
Sbjct: 47 IIGNLLQMG-KNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGR 105
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ +I A +C+ W+ LR I ELFS +A+E E R +KV E++
Sbjct: 106 YLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELV 165
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSE---EMQRIVRWIVECGA- 693
+FL +K GE++K+ D +F ++ NILS S D+ ++ ++ ++ E GA
Sbjct: 166 EFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGAT 225
Query: 694 PGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALI 870
P L D YP L G D I ++A + E W DIL ER+ + + +DFL+AL+
Sbjct: 226 PNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSH--RDFLEALL 283
Query: 871 KNSWDDNEIFTFLSEFF---VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAE 1041
+ ++D++I + E F T SLT +EWAM EL+RNQ A+ KL+ EL + + E
Sbjct: 284 EIGFEDDQINQVILELFSAGAETSSLT--VEWAMAELIRNQDAMDKLRGELRQIV---GE 338
Query: 1042 IVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYAL 1221
+ E L +LPYLQAC+KE RLHPP+P L+P A +TC+VM Y IPK++ + +N +A+
Sbjct: 339 SPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAM 398
Query: 1222 GRDSMTWDDPLSFKPER 1272
RD WDDPLSFKPER
Sbjct: 399 ARDPKIWDDPLSFKPER 415
>ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 487
Score = 275 bits (702), Expect = 4e-71
Identities = 154/375 (41%), Positives = 224/375 (59%), Gaps = 7/375 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
++GNI L K PHIS+AK AE GPL S+K+G++ ++AS+P SA EI K D+ LS R
Sbjct: 40 VLGNILQLG-KNPHISLAKFAEIHGPLISVKLGTQLVVVASSPHSAAEILKTQDRLLSAR 98
Query: 349 YIPQINRI-LPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
P + L +I + + W+FLR T LFS +A+E + R KKV+EM+
Sbjct: 99 SPPSVVPYELSVIDQHSIVFSSDLGNHWKFLRAFCRTHLFSPKAVESQAALRDKKVSEMI 158
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWN---DSEEMQRIVRWIVECGA- 693
FL SK GE +KI + +F ++ N L + S+D+V + + ++R+ R +E GA
Sbjct: 159 HFLRSKKGETVKISEILFGTILNTLGNLFFSKDLVCGLDYERNPSGIKRVFRKYIELGAM 218
Query: 694 PGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALI 870
P + D YP D ++K+ ++ +W +I+ E K+Q DFLD +I
Sbjct: 219 PNISDFYPLFEALDLQGLRKQTEIYQSRLINIWSEIVKE----KRQAISRHSSDFLDVMI 274
Query: 871 KNSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIV 1047
+ + D +I ++E +++ T+ IEWAMTEL+RN A+ KLQ ELT I
Sbjct: 275 DSGFSDLQINILVTELMGAGSDTTTSTIEWAMTELLRNNGAMHKLQAELTSKFGENDTIT 334
Query: 1048 LNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGR 1227
E +S+LPYL AC+KET RLH P+PFL+PR A +TC++M Y IPKN+ + +NAYA+GR
Sbjct: 335 --ESNISELPYLAACVKETLRLHSPTPFLIPRRAPETCKIMNYTIPKNSKLFVNAYAIGR 392
Query: 1228 DSMTWDDPLSFKPER 1272
DS TW+D LSFKPER
Sbjct: 393 DSNTWEDALSFKPER 407
>ref|XP_002276320.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 571
Score = 275 bits (702), Expect = 4e-71
Identities = 153/379 (40%), Positives = 237/379 (62%), Gaps = 11/379 (2%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ H K+ HI++A A S+GPLFSL++G++ I+ S+ +A+EI +D+ R
Sbjct: 114 IIGNV-HQIGKQRHIAMADFARSYGPLFSLRLGTQTLIVGSSAAAAKEILSSYDRIFCAR 172
Query: 349 YIPQINRILPETKT----SAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVN 516
Y+P + +PE + ++I + ECD+RW++LRT+ T+LFS +A+E + R KK+
Sbjct: 173 YVPGV---MPEKSSEFYNNSIVWSLECDDRWKYLRTMCRTQLFSGKAIESQACLREKKLM 229
Query: 517 EMLDFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIVEC--- 687
E++ FL+S G ++K+K+ F + N++S ++S+D+V S D + R++ + +
Sbjct: 230 EVVGFLSSMEGRVVKLKELAFVTALNMISNALLSKDLV-SLEDETAVARMLGCVKKTVDV 288
Query: 688 -GAPGLFDLYPALSGFDFWI--KKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFL 858
P L D YP L G D KK + F E+ +W I+ ER+ K + DFL
Sbjct: 289 MSTPNLADYYPILRGLDLQRLQKKSRDSFVELFS-LWQPIVKERRERKGSHATRQ-HDFL 346
Query: 859 DALIKNSWDDNEIFTFLSEFFVS-TESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHK 1035
DALI + + D+ I L E ++ TES + EWAM EL+R+ ++ K+++ELT I+
Sbjct: 347 DALINDGFTDDRINFLLGELLIAGTESTSVTTEWAMAELIRSPDSMKKIREELTTEINKS 406
Query: 1036 AEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAY 1215
L + L KLPYLQAC+KET RLHPP PFL+P AL++C+VM Y IPK+ V++NA+
Sbjct: 407 T---LKDSDLRKLPYLQACLKETLRLHPPGPFLLPHRALESCKVMNYTIPKDAQVLVNAW 463
Query: 1216 ALGRDSMTWDDPLSFKPER 1272
A+GRD M+W+DPL FKPER
Sbjct: 464 AIGRDPMSWEDPLVFKPER 482
>emb|CBI34742.3| unnamed protein product [Vitis vinifera]
Length = 504
Score = 275 bits (702), Expect = 4e-71
Identities = 153/379 (40%), Positives = 237/379 (62%), Gaps = 11/379 (2%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ H K+ HI++A A S+GPLFSL++G++ I+ S+ +A+EI +D+ R
Sbjct: 47 IIGNV-HQIGKQRHIAMADFARSYGPLFSLRLGTQTLIVGSSAAAAKEILSSYDRIFCAR 105
Query: 349 YIPQINRILPETKT----SAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVN 516
Y+P + +PE + ++I + ECD+RW++LRT+ T+LFS +A+E + R KK+
Sbjct: 106 YVPGV---MPEKSSEFYNNSIVWSLECDDRWKYLRTMCRTQLFSGKAIESQACLREKKLM 162
Query: 517 EMLDFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIVEC--- 687
E++ FL+S G ++K+K+ F + N++S ++S+D+V S D + R++ + +
Sbjct: 163 EVVGFLSSMEGRVVKLKELAFVTALNMISNALLSKDLV-SLEDETAVARMLGCVKKTVDV 221
Query: 688 -GAPGLFDLYPALSGFDFWI--KKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFL 858
P L D YP L G D KK + F E+ +W I+ ER+ K + DFL
Sbjct: 222 MSTPNLADYYPILRGLDLQRLQKKSRDSFVELFS-LWQPIVKERRERKGSHATRQ-HDFL 279
Query: 859 DALIKNSWDDNEIFTFLSEFFVS-TESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHK 1035
DALI + + D+ I L E ++ TES + EWAM EL+R+ ++ K+++ELT I+
Sbjct: 280 DALINDGFTDDRINFLLGELLIAGTESTSVTTEWAMAELIRSPDSMKKIREELTTEINKS 339
Query: 1036 AEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAY 1215
L + L KLPYLQAC+KET RLHPP PFL+P AL++C+VM Y IPK+ V++NA+
Sbjct: 340 T---LKDSDLRKLPYLQACLKETLRLHPPGPFLLPHRALESCKVMNYTIPKDAQVLVNAW 396
Query: 1216 ALGRDSMTWDDPLSFKPER 1272
A+GRD M+W+DPL FKPER
Sbjct: 397 AIGRDPMSWEDPLVFKPER 415
>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 496
Score = 274 bits (701), Expect = 5e-71
Identities = 141/374 (37%), Positives = 236/374 (63%), Gaps = 6/374 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
++GNIF + K PHI++ A+++GPL SL++G++ ++ S+P+ A EIFK HD+ LSGR
Sbjct: 44 VIGNIFQIG-KMPHITLTNFAKTYGPLISLRLGTQCLVVGSSPSVAIEIFKTHDRILSGR 102
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
++P R + + +++ ECD WR+LRT+ T+LF+ + LE + R KKV E++
Sbjct: 103 HVPNAVRSKGSDLEKTSLGWKSECDSGWRYLRTLCRTDLFAGKVLESQACLREKKVMELV 162
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSE--EMQRIVRWIVECG-AP 696
+FL SK G+++ I + +FA+V N+LS +++S+D++ ++E E++ +VR IVE G AP
Sbjct: 163 EFLRSKEGQVVNIGELVFATVLNMLSNVLISKDMIHLEEETEDSEIKSLVRGIVEVGSAP 222
Query: 697 GLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALIK 873
+ D YP L D ++K++ D +W I+ ER+ ++ + QDFL+ L+
Sbjct: 223 NISDFYPILGKLDLQGLRKKSTDLVTKFCSIWEPIIEERRNSRENDSSSQ-QDFLETLLD 281
Query: 874 NSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIVL 1050
N + ++ I E +++ TT IEWAM EL++N + K+Q+EL I+ L
Sbjct: 282 NGFTNDHINQLFMELLSAGSDTSTTAIEWAMAELIKNVETMKKVQEELE--IELSESDYL 339
Query: 1051 NEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGRD 1230
E + ++ Y+QAC+KE FRLHPP+P L+P A++TC+VM Y IPK +++N +A+ RD
Sbjct: 340 KESQVLQMSYVQACVKEAFRLHPPAPLLLPHRAIETCQVMSYTIPKGAQILVNVWAITRD 399
Query: 1231 SMTWDDPLSFKPER 1272
W++P F+P+R
Sbjct: 400 PFIWEEPDMFRPQR 413
>ref|XP_006340577.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 503
Score = 274 bits (700), Expect = 6e-71
Identities = 150/380 (39%), Positives = 231/380 (60%), Gaps = 12/380 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLF-SLKIGSRFFIIASNPTSAREIFKIHDKELSG 345
++GN+ + +PH S+AKLA+ GP F S++ G+R ++AS+P +A E+ K HD+ LSG
Sbjct: 44 LIGNLLQIGKTRPHASLAKLAQVHGPDFMSIRFGTRLIVVASSPAAAAEVLKTHDRVLSG 103
Query: 346 RYIPQINRILPE-TKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEM 522
RY+ ++ RI + A ECD+ W+ +RTI LFS++ALE +R KKV EM
Sbjct: 104 RYVSRVVRIKGSIVHNLSTAFLEECDDNWKKVRTIYRGALFSTKALESQVSTREKKVMEM 163
Query: 523 LDFLNS-KNGELIKIKDFIFASVTNILSRMMVSEDIV--KSWNDSEEMQRIVRWIVECGA 693
+ +L + ++I+I++ IF +V NIL +++S D++ + EEM + +R E GA
Sbjct: 164 IRYLEEIQLNQVIQIREVIFVTVLNILGNLLLSMDLIDFEGRGVGEEMMKYLREFTETGA 223
Query: 694 PG-LFDLYPALSG-----FDFWIKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDF 855
L D++P L F KK + F + + +W ++ ER++ + Q ++ DF
Sbjct: 224 TQELADMFPVLGAICSMNFQRTYKKIMDLFNKTSV-VWAGVVQERRKRESQTS-MDAVDF 281
Query: 856 LDALIKNSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDH 1032
+DAL+KN + D I L E F TE+ EW + EL++N L KL+DE+ +
Sbjct: 282 VDALVKNGFTDKHINALLMELFGAGTETTIVTSEWMLVELLKNHQCLTKLRDEIANVVGD 341
Query: 1033 KAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNA 1212
K ++++ E L+ +PYL AC+KET RLHPP P L+P A++TCEVM YRIPK+T V++N
Sbjct: 342 K-DVIIRESDLTNMPYLDACLKETLRLHPPGPLLLPHRAVETCEVMGYRIPKDTQVMVNM 400
Query: 1213 YALGRDSMTWDDPLSFKPER 1272
+A+ RD WDDP SFKPER
Sbjct: 401 WAIARDPKVWDDPSSFKPER 420
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 273 bits (699), Expect = 8e-71
Identities = 145/374 (38%), Positives = 230/374 (61%), Gaps = 6/374 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GNI + K PHIS+A A+ GPL SL++G+R ++AS+PT+A EI K HD+ LSGR
Sbjct: 46 ILGNILQVG-KNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGR 104
Query: 349 YIPQINRILPETKTS-AIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
YIP A+ C ++W+FLR + +ELFS++A+E + R KK+ EML
Sbjct: 105 YIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEML 164
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIV--KSWNDSEEMQRIVRWIVECGA-P 696
DFL SK G+++ I + +F + N +S ++ S+D++ + ++ E++ ++ ++E P
Sbjct: 165 DFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCP 224
Query: 697 GLFDLYPALSGFD-FWIKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALIK 873
+ D YP L+ D IK++ + E +W + ER+ ++ +D DFLD +
Sbjct: 225 NIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERR--ERHVKDARKTDFLDVFLS 282
Query: 874 NSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIVL 1050
N +DD++I L E F ++ TT +EWAM EL++ L K+++EL I + ++
Sbjct: 283 NGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEI---GKDMI 339
Query: 1051 NEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGRD 1230
E + +L YL AC+KET RLHPP PFL+PR A + CEVM Y IPK++ +++N +A+GRD
Sbjct: 340 RESHIPQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRD 399
Query: 1231 SMTWDDPLSFKPER 1272
W+DPLS++PER
Sbjct: 400 PSAWEDPLSYRPER 413
>ref|XP_002318835.2| hypothetical protein POPTR_0012s13630g [Populus trichocarpa]
gi|550327063|gb|EEE97055.2| hypothetical protein
POPTR_0012s13630g [Populus trichocarpa]
Length = 507
Score = 273 bits (698), Expect = 1e-70
Identities = 145/381 (38%), Positives = 230/381 (60%), Gaps = 13/381 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + KPHIS+ KLA+ +GPL SL++G++ ++ S+ +A EI K HD+ELSGR
Sbjct: 49 IIGNVLQMG-NKPHISLTKLAQVYGPLMSLRLGTQLVVVGSSREAASEILKTHDRELSGR 107
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
+P + P+ +IA EC +RWRF R++ ELFSS+ ++ S +R K EM+
Sbjct: 108 CVPHASFAKDPKLNEDSIAWTFECTDRWRFFRSLMRNELFSSKVVDGQSRTRETKAREMI 167
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRI---VRWIVECGAP 696
DFL K GE +KI+D +F N L+ + +S+D+V ++ E QR+ VR ++E
Sbjct: 168 DFLKKKEGEGVKIRDIVFVYTFNALANIYLSKDLV-DYDQIGECQRVCGLVREMMELHTT 226
Query: 697 -GLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVEC-----QDF 855
+ DLYP L D + ++ N+ + +WG ++ ER+ + D + +DF
Sbjct: 227 LNISDLYPILGSLDLQGVSRKCNECESRIQELWGSVIKERREGRNDTGDDDDNSSKRKDF 286
Query: 856 LDALIKNSWDDNEIFTFLSEFFVS--TESLTTIIEWAMTELMRNQVALIKLQDELTKAID 1029
LD L+ + D +I F + ++ ++S ++ ++WAM ELMRN A+ +L++EL
Sbjct: 287 LDVLLDGEFSDEQISLFFVQELLAAVSDSTSSTVDWAMAELMRNPQAMKQLREELA---G 343
Query: 1030 HKAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLN 1209
E ++ E L+K PYL C+KET RLHPP+PFL+P A + C+V+ IPK+T V++N
Sbjct: 344 ETPEDLITESSLAKFPYLHLCVKETLRLHPPAPFLIPHRATEDCQVLDCTIPKDTQVLVN 403
Query: 1210 AYALGRDSMTWDDPLSFKPER 1272
+A+ RD +W+DPL FKPER
Sbjct: 404 VWAIARDPASWEDPLCFKPER 424
>ref|XP_002318834.2| hypothetical protein POPTR_0012s13620g [Populus trichocarpa]
gi|550327062|gb|EEE97054.2| hypothetical protein
POPTR_0012s13620g [Populus trichocarpa]
Length = 506
Score = 271 bits (694), Expect = 3e-70
Identities = 145/380 (38%), Positives = 231/380 (60%), Gaps = 12/380 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + KPHIS+ KLA+ +GPL SL++G++ ++ S+ +A EI K HD+ELSGR
Sbjct: 49 IIGNVLQIG-NKPHISLTKLAQVYGPLMSLRLGTQLVVVGSSREAASEILKTHDRELSGR 107
Query: 349 YIPQINRIL-PETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
+P + P+ +IA EC +RWRF R++ ELFSS+ ++ S +R K EM+
Sbjct: 108 CVPHASFAKDPKLNEDSIAWTFECTDRWRFFRSLMRNELFSSKVVDGQSSTRETKAKEMI 167
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRI---VRWIVECGAP 696
DFL K GE +KI+D +F N+L+ + +S+D++ ++ + E QR+ VR ++E
Sbjct: 168 DFLKKKEGEGVKIRDIVFVYTFNVLANIYLSKDLI-DYDQTGECQRVCGLVREMMELHTT 226
Query: 697 -GLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVEC----QDFL 858
+ DLYP L D + ++ N+ + +W I+ ER+ + D + +DFL
Sbjct: 227 LNISDLYPILGSLDLQGLSRKTNECGSRIQELWRSIIKERREGRNDTGDDDNSSKRKDFL 286
Query: 859 DALIKNSWDDNEIFTFLSEFFVS--TESLTTIIEWAMTELMRNQVALIKLQDELTKAIDH 1032
D L+ + D +I +F + ++ ++S ++ IEWAM ELMRN A+ +L++EL
Sbjct: 287 DVLLDGEFSDEQISSFFVQELLAAVSDSSSSTIEWAMAELMRNPQAMKQLREELA---GE 343
Query: 1033 KAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNA 1212
E ++ E L+K PYL C+KET RLHPP+P L+P A + C+V+ IPK+T V++N
Sbjct: 344 TPEDLITESSLAKFPYLHLCVKETLRLHPPAPLLIPHRATEDCQVLDCTIPKDTQVLVNV 403
Query: 1213 YALGRDSMTWDDPLSFKPER 1272
+A+ RD +W+DPL FKPER
Sbjct: 404 WAIARDPASWEDPLCFKPER 423
>ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Solanum lycopersicum]
Length = 486
Score = 271 bits (693), Expect = 4e-70
Identities = 143/374 (38%), Positives = 230/374 (61%), Gaps = 6/374 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
++GN+ + K PH+S+AK A+ GPL S+K+G++ ++AS+P SA EI K D+ LS R
Sbjct: 40 LLGNLLQIG-KTPHVSLAKFAQIHGPLISVKLGTQLVVVASSPASAAEILKSQDRLLSVR 98
Query: 349 YIPQINRI-LPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
+P++ L +I + + +W+FLR T LFS +A+E + R KKV+EM+
Sbjct: 99 SVPKVATYELSVIDQHSIVFSSDLSNQWKFLRAFCRTHLFSPKAVESQAALREKKVSEMM 158
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDI--VKSWNDSEEMQRIVRWIVECGA-P 696
+FL S+ GE +KI + +F ++ N L + S+D+ + ++ ++ ++R VE GA P
Sbjct: 159 NFLRSRKGETVKISEILFGTILNTLGNLFFSKDLCDLDYETNTSGIKHVIRKFVELGAMP 218
Query: 697 GLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALIK 873
+ + YP D ++K+ ++ +W +I+ E K+Q DFLDA+I
Sbjct: 219 NISEFYPLFDALDLQGLRKQTEIYQNRLVNIWSEIVKE----KRQAISRSSSDFLDAMID 274
Query: 874 NSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIVL 1050
N + D ++ L E +++ T+ IEWAM EL+RN+ A+ KL+ EL + ++
Sbjct: 275 NGFSDLQVDILLMELISAGSDTTTSTIEWAMAELLRNKGAMRKLKAELASKLGEND--II 332
Query: 1051 NEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGRD 1230
E +S+LPYL AC+KET R+HPP+PFL+PR A +TC++M Y IPKN+ + +NA+A+GRD
Sbjct: 333 TESNVSELPYLAACVKETLRIHPPTPFLIPRRAPETCKIMDYTIPKNSKLFVNAWAIGRD 392
Query: 1231 SMTWDDPLSFKPER 1272
S TW+D LSF+PER
Sbjct: 393 SNTWEDALSFRPER 406
>ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Vitis vinifera]
Length = 498
Score = 271 bits (692), Expect = 5e-70
Identities = 153/380 (40%), Positives = 225/380 (59%), Gaps = 12/380 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + H+ +A LA+ GPL SL++G++ ++AS+ +A E+ K HD+ LSGR
Sbjct: 47 IIGNLLQMGGNL-HVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGR 105
Query: 349 YIPQINRI-LPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ A+A A C+ WR L+ I ELFS +A+E E R +KV E++
Sbjct: 106 YVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELV 165
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIV-------- 681
+FL K GE++K+ D ++ +V NILS S D S+ R VR ++
Sbjct: 166 EFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDF------SDFEGRDVRGVLLKDLLNEN 219
Query: 682 -ECGAPGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDF 855
E GA + D YP L G D I+K+ + W DIL ER+ KQ+ DF
Sbjct: 220 AELGATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDF 277
Query: 856 LDALIKNSWDDNEIFTFLSE-FFVSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDH 1032
LDAL++ ++D++I + E FF E+ + +EWAM EL++NQ A+ KL +ELT+ I
Sbjct: 278 LDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQII-- 335
Query: 1033 KAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNA 1212
E + E L LPYLQAC+KET RLHP P L+P A +TC++M Y IPK++++ +N
Sbjct: 336 -GESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNM 394
Query: 1213 YALGRDSMTWDDPLSFKPER 1272
+A+GRD TW+DPLSFKPER
Sbjct: 395 WAMGRDPGTWEDPLSFKPER 414
>emb|CAN70170.1| hypothetical protein VITISV_006874 [Vitis vinifera]
Length = 559
Score = 271 bits (692), Expect = 5e-70
Identities = 153/380 (40%), Positives = 225/380 (59%), Gaps = 12/380 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
I+GN+ + H+ +A LA+ GPL SL++G++ ++AS+ +A E+ K HD+ LSGR
Sbjct: 47 IIGNLLQMGGNL-HVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRTLSGR 105
Query: 349 YIPQINRI-LPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
Y+ + P+ A+A A C+ WR L+ I ELFS +A+E E R +KV E++
Sbjct: 106 YVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKVMELV 165
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEEMQRIVRWIV-------- 681
+FL K GE++K+ D ++ +V NILS S D S+ R VR ++
Sbjct: 166 EFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDF------SDFEGRDVRGVLLKDLLNEN 219
Query: 682 -ECGAPGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDF 855
E GA + D YP L G D I+K+ + W DIL ER+ KQ+ DF
Sbjct: 220 AELGATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR--KQRIHGSSHGDF 277
Query: 856 LDALIKNSWDDNEIFTFLSE-FFVSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDH 1032
LDAL++ ++D++I + E FF E+ + +EWAM EL++NQ A+ KL +ELT+ I
Sbjct: 278 LDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQII-- 335
Query: 1033 KAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNA 1212
E + E L LPYLQAC+KET RLHP P L+P A +TC++M Y IPK++++ +N
Sbjct: 336 -GESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNM 394
Query: 1213 YALGRDSMTWDDPLSFKPER 1272
+A+GRD TW+DPLSFKPER
Sbjct: 395 WAMGRDPGTWEDPLSFKPER 414
>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 486
Score = 270 bits (691), Expect = 7e-70
Identities = 145/374 (38%), Positives = 229/374 (61%), Gaps = 6/374 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
++GNI + K PHIS+A+ A+ +GPL S+K+G++ ++AS+P SA EI K D+ LS R
Sbjct: 40 LLGNILQIG-KSPHISLAQFAKIYGPLISVKLGTQLVVVASSPDSAAEILKTQDRLLSVR 98
Query: 349 YIPQINRI-LPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEML 525
+P++ L +I + + W+FLR T LFS +A+E + R KKV+EM+
Sbjct: 99 SVPKVATYELSVIDQHSIVFSSDLSNHWKFLRAFCRTHLFSPKAVESQAALREKKVSEMI 158
Query: 526 DFLNSKNGELIKIKDFIFASVTNILSRMMVSEDI--VKSWNDSEEMQRIVRWIVECGA-P 696
FL S+ GE +KI + +FA++ N L + S+D+ + ++ ++ ++R VE GA P
Sbjct: 159 HFLRSRKGETVKISEIMFATILNTLGNLFFSKDLCDLDYETNTSGIKHVIRKFVELGAMP 218
Query: 697 GLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALIK 873
+ + YP D ++K+ ++ +W DI+ E K+Q DFLD +I
Sbjct: 219 NISEFYPLFDALDLQGLRKQTEIYQNQLFNIWSDIVKE----KRQAISRSSSDFLDVMID 274
Query: 874 NSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIVL 1050
+ + D ++ L E +++ T+ IEWAM EL+RN+ A+ KLQ ELT I +L
Sbjct: 275 SGFSDLQVDILLMELISAGSDTTTSTIEWAMAELLRNKGAMHKLQAELTSKIGEND--IL 332
Query: 1051 NEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGRD 1230
E +++LPYL AC+KET R+HPP+PFL+PR A +TC++M Y IPKN+ + +N +A+GR+
Sbjct: 333 TESNITELPYLAACVKETLRIHPPTPFLIPRRAPETCKIMDYTIPKNSRLFVNVWAIGRN 392
Query: 1231 SMTWDDPLSFKPER 1272
S TW+D LSF+PER
Sbjct: 393 SNTWEDALSFRPER 406
>gb|ABD39696.1| littorine mutase/monooxygenase [Hyoscyamus niger]
Length = 510
Score = 270 bits (690), Expect = 9e-70
Identities = 155/382 (40%), Positives = 233/382 (60%), Gaps = 14/382 (3%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGP-LFSLKIGSRFFIIASNPTSAREIFKIHDKELSG 345
I+G++F+L KPH+S+AKLA GP L S+++G R I+AS+P + E+ K HD+ LSG
Sbjct: 48 IIGHLFYLG-NKPHVSLAKLANVHGPHLMSIRLGGRLVIVASSPMATAEVLKTHDRLLSG 106
Query: 346 RYIPQINRILPE-TKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEM 522
R++ R+ + A ECDE W+ +R++ LFS +A+E R KKV E+
Sbjct: 107 RFVSHPMRVEGSYIRNLATETLEECDENWKKVRSMYQIVLFSHKAVESQVNIREKKVMEL 166
Query: 523 LDFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIV--KSWNDSEEMQRIVRWIVEC-GA 693
+ F+ SK GEL+ IK F ++ NILS +S D+V + E M+ +R + G
Sbjct: 167 VKFVASKEGELVNIKGIAFVTILNILSNSTISNDLVDFEGKGIGEGMREWIRNYTKLEGV 226
Query: 694 PGLFDLYPALSG----FDFWIKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLD 861
P L DL+P L G F KK + F I+ +W DI++ +KRM+ + E +DF D
Sbjct: 227 PQLADLFPILDGCTWDFQGTYKKLKDTFERISD-VWRDIIN-KKRMEISNKYYEGEDFAD 284
Query: 862 ALIKNSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTK---AID 1029
ALI+N ++D +I L E + TE+ T +EW + EL++N A+ +L++E+ K ID
Sbjct: 285 ALIRNGFEDKQINALLMELYSAGTETTITTVEWTLVELLKNPEAMKRLRNEIKKELTTID 344
Query: 1030 HKAEIVLNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLN 1209
+ +++ + L LPYL+AC+KET RLHPP+P L P A+QTCEVM YRIP++T +++N
Sbjct: 345 DREIMIVKDSNLPNLPYLEACMKETLRLHPPAPLLFPHRAVQTCEVMGYRIPQDTQIIVN 404
Query: 1210 AYALGRDSMTW-DDPLSFKPER 1272
+ + RDS W DDP SFKP+R
Sbjct: 405 VWKMARDSEYWNDDPWSFKPDR 426
>ref|XP_006358915.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 495
Score = 269 bits (688), Expect = 2e-69
Identities = 146/375 (38%), Positives = 237/375 (63%), Gaps = 7/375 (1%)
Frame = +1
Query: 169 IVGNIFHLDPKKPHISIAKLAESFGPLFSLKIGSRFFIIASNPTSAREIFKIHDKELSGR 348
++GNIF + K PHI++ A+++GPL SLK+G+++ ++ S+P +A EI K HDK LSGR
Sbjct: 43 VIGNIFQMG-KMPHITLTNFAKTYGPLMSLKLGTKWLVVGSSPLAAVEILKTHDKILSGR 101
Query: 349 YIPQI--NRILPETKTSAIAMAGECDERWRFLRTIAHTELFSSQALEYGSESRLKKVNEM 522
+P ++ KTS + EC+ WR+LRT+ TELF+ + LE + R KKV E+
Sbjct: 102 DVPSAAPSKGSDLDKTS-LGWISECNSGWRYLRTLCRTELFAGKVLESQACLREKKVMEL 160
Query: 523 LDFLNSKNGELIKIKDFIFASVTNILSRMMVSEDIVKSWNDSEE--MQRIVRWIVEC-GA 693
++FL SK G+++ I + +FA+V N+LS +++S+D+V + E+ ++ +VR IVE A
Sbjct: 161 VEFLRSKEGQVVNIGELVFATVLNMLSNVLISKDMVNLEEEIEDGVIKSLVRGIVEVLSA 220
Query: 694 PGLFDLYPALSGFDFW-IKKRANDFREINKRMWGDILSERKRMKQQKQDVECQDFLDALI 870
P + DLYP L D ++K++ D W IL ER+ +++ + QDFL+ L+
Sbjct: 221 PNISDLYPILGKLDLQGLQKKSIDLVTKLCSNWEPILEERRNSREKGSSSQ-QDFLETLL 279
Query: 871 KNSWDDNEIFTFLSEFF-VSTESLTTIIEWAMTELMRNQVALIKLQDELTKAIDHKAEIV 1047
N + ++ I L E +++ T+ IEWAM ELM+N ++ K+Q+EL I+
Sbjct: 280 DNGFTNDRIHQLLMELLSAGSDTSTSTIEWAMAELMKNVESMKKVQEELE--IELSESDY 337
Query: 1048 LNEECLSKLPYLQACIKETFRLHPPSPFLVPRSALQTCEVMKYRIPKNTVVVLNAYALGR 1227
L E L ++ Y+QAC+KET RLHPP P L+P A+++C+VM Y IPK+ +++N +A+ R
Sbjct: 338 LKESQLLQMSYIQACVKETLRLHPPGPLLLPHRAIESCQVMSYTIPKDAQILVNIWAITR 397
Query: 1228 DSMTWDDPLSFKPER 1272
D + W++P F+P+R
Sbjct: 398 DPLIWEEPDMFRPQR 412