BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Catharanthus22_contig00008669
         (2131 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283797.1| PREDICTED: ubiquitin-like modifier-activatin...   656   0.0  
gb|EOY22484.1| NAD(P)-binding Rossmann-fold superfamily protein ...   643   0.0  
ref|XP_006351469.1| PREDICTED: ubiquitin-like modifier-activatin...   642   0.0  
ref|XP_004236327.1| PREDICTED: ubiquitin-like modifier-activatin...   640   0.0  
ref|XP_002511184.1| Ubiquitin-activating enzyme E1 domain-contai...   638   e-180
gb|EMJ11706.1| hypothetical protein PRUPE_ppa006127mg [Prunus pe...   636   e-179
ref|XP_002321704.1| thiF family protein [Populus trichocarpa] gi...   634   e-179
ref|XP_004297542.1| PREDICTED: ubiquitin-like modifier-activatin...   633   e-178
gb|ESW27544.1| hypothetical protein PHAVU_003G211200g [Phaseolus...   632   e-178
ref|XP_004145156.1| PREDICTED: ubiquitin-like modifier-activatin...   630   e-178
ref|XP_006485349.1| PREDICTED: ubiquitin-like modifier-activatin...   627   e-177
ref|XP_003549854.2| PREDICTED: ubiquitin-like modifier-activatin...   626   e-176
ref|XP_004501700.1| PREDICTED: ubiquitin-like modifier-activatin...   626   e-176
ref|XP_003525714.2| PREDICTED: ubiquitin-like modifier-activatin...   626   e-176
ref|XP_006418036.1| hypothetical protein EUTSA_v10007699mg [Eutr...   626   e-176
ref|XP_003602810.1| Ubiquitin-like modifier-activating enzyme [M...   622   e-175
ref|NP_172027.2| NAD(P)-binding Rossmann-fold superfamily protei...   619   e-174
ref|XP_003595421.1| Ubiquitin-like modifier-activating enzyme [M...   619   e-174
gb|ACU21220.1| unknown [Glycine max]                                  616   e-173
ref|XP_006436836.1| hypothetical protein CICLE_v10031478mg [Citr...   610   e-172

>ref|XP_002283797.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5 isoform 1
            [Vitis vinifera] gi|297734421|emb|CBI15668.3| unnamed
            protein product [Vitis vinifera]
          Length = 427

 Score =  656 bits (1692), Expect = 0.0
 Identities = 335/426 (78%), Positives = 369/426 (86%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            M+ ELK +LDDL+ L+ S  DPS QA IDK++  V+++TNLA+     R+KVK++SAEVV
Sbjct: 1    MDVELKEMLDDLQALKRSLPDPSHQASIDKLQVHVEHLTNLAQYAPARRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETF SSLR K+
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLRKKT 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P KEGSGV LVLSCVDNYEARMVVNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPKKEGSGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK+LL FG V+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAE--VLTEGPLHEE 648
            NALKD+FPTMEM+PNPQCSN++CLERQKEY+LAKPARDAAA+ KM AE  V+ E P+H +
Sbjct: 301  NALKDYFPTMEMRPNPQCSNASCLERQKEYLLAKPARDAAAKAKMEAEALVVAECPIHVD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESS-NDADDLEDLKRQ 471
            NEWNISVVDD++L   D R S ALPEGLTHELP ADEFEK P  E++ N  DDL+DL+RQ
Sbjct: 361  NEWNISVVDDSELDRTDGRSSDALPEGLTHELPTADEFEKQPTPETTFNSVDDLDDLRRQ 420

Query: 470  LEALNA 453
            L+ALNA
Sbjct: 421  LDALNA 426


>gb|EOY22484.1| NAD(P)-binding Rossmann-fold superfamily protein [Theobroma cacao]
          Length = 428

 Score =  643 bits (1658), Expect = 0.0
 Identities = 334/427 (78%), Positives = 364/427 (85%), Gaps = 4/427 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +LDDL+ L+ S  DP+  A IDK++S VD++T+LAKS  + R+KVK++SAEVV
Sbjct: 1    MEVELKEMLDDLQSLRQSLSDPTLHASIDKLQSCVDHLTHLAKSVPVRRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETF SSLRNK+
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLRNKT 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+KEGSGV LVLSCVDNYEARMVVNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FCPSKEGSGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK+LL FG V+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLTEG---PLHE 651
            ++LKD+FPTM MKPNPQCSN+ACLERQKEYILAKPARDA A+ KM AE  T     PLH 
Sbjct: 301  SSLKDYFPTMAMKPNPQCSNAACLERQKEYILAKPARDAEAKAKMEAEASTAAADLPLHA 360

Query: 650  ENEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAES-SNDADDLEDLKR 474
            +NEWNISVVDD+ L       S ALPEGL HELP ADEF+K PA+E+      DLEDL+R
Sbjct: 361  DNEWNISVVDDSGLESTGGTSSDALPEGLIHELPSADEFQKPPASEALDTGIVDLEDLRR 420

Query: 473  QLEALNA 453
            QLEALNA
Sbjct: 421  QLEALNA 427


>ref|XP_006351469.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Solanum
            tuberosum]
          Length = 426

 Score =  642 bits (1656), Expect = 0.0
 Identities = 325/425 (76%), Positives = 356/425 (83%), Gaps = 2/425 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +L DL  L+ S  DPS  ALI+KM    +N+  L KSGT  R+KVK++SAEVV
Sbjct: 1    MEVELKELLSDLHSLKKSISDPSHLALIEKMHLHAENIATLEKSGTARRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVNNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQVGMTKTDAAVQTLS+INPDVVLESYTLNITTV+GF+TF SSL NKS
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLSEINPDVVLESYTLNITTVEGFDTFMSSLTNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P K GSGV LVLSCVDNYEARMVVNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FQPTKSGSGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFAC PPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN+LKYLL FGQV+ YLGY
Sbjct: 241  CFACVPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLKFGQVSRYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT--EGPLHEE 648
            NALKD+FPTMEMKPN QCSN+ACLERQKEYILAKP RDAAA+ K   E L+  E P+H +
Sbjct: 301  NALKDYFPTMEMKPNSQCSNAACLERQKEYILAKPTRDAAAKAKADIEALSPAENPVHAD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQL 468
            NEWNISVVDD ++  +D + SG LPEG+ HELP+ADE+ K P  E+   ADDLE+L+RQL
Sbjct: 361  NEWNISVVDDEEVDGSDVKISGVLPEGIVHELPVADEYPKPPVTETVGPADDLEELRRQL 420

Query: 467  EALNA 453
            EALNA
Sbjct: 421  EALNA 425


>ref|XP_004236327.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Solanum
            lycopersicum]
          Length = 426

 Score =  640 bits (1651), Expect = 0.0
 Identities = 323/425 (76%), Positives = 357/425 (84%), Gaps = 2/425 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +L DL  L+ S  DPS  ALI+KM    +N+  L KSGT+ R+KVK++SAEVV
Sbjct: 1    MEVELKELLSDLHSLKKSISDPSHLALIEKMHLHAENIATLEKSGTIRRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVNNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQVGMTKTDAAVQTLS+INPDVVLESYTLNITTV+GF+TF SSL NKS
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLSEINPDVVLESYTLNITTVEGFDTFMSSLTNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P K GSGV LVLSCVDNYEARMVVNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPTKSGSGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFAC PPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN+LKYLL FGQV+ YLGY
Sbjct: 241  CFACVPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLKFGQVSRYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT--EGPLHEE 648
            NALKD+FPTMEMKPN QCSN+ACLERQKEYILAKP RDAAA+ K   E ++  E P+H +
Sbjct: 301  NALKDYFPTMEMKPNSQCSNAACLERQKEYILAKPTRDAAAKAKADIEAVSPAENPVHAD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQL 468
            NEWNISVVDD ++  +D + SG LPEG+ HELP+AD++ K P  E+   ADDLE+L+RQL
Sbjct: 361  NEWNISVVDDEEVDGSDVKNSGVLPEGIVHELPVADDYPKAPVTETVGPADDLEELRRQL 420

Query: 467  EALNA 453
            EALNA
Sbjct: 421  EALNA 425


>ref|XP_002511184.1| Ubiquitin-activating enzyme E1 domain-containing protein, putative
            [Ricinus communis] gi|223550299|gb|EEF51786.1|
            Ubiquitin-activating enzyme E1 domain-containing protein,
            putative [Ricinus communis]
          Length = 428

 Score =  638 bits (1645), Expect = e-180
 Identities = 329/426 (77%), Positives = 364/426 (85%), Gaps = 4/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELKG+LDDL  L +S  D S+QA I K++SRV+ +T LA S    R+KVK++SAEVV
Sbjct: 1    MEAELKGLLDDLSSLISSLPDASRQASIHKLQSRVEVITKLATSAPSRRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVHNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVGMTKTDAAVQTLS+INPDVVLESYT+NITTVQGFETF SSL++KS
Sbjct: 121  ELANMNRLFFRPDQVGMTKTDAAVQTLSEINPDVVLESYTMNITTVQGFETFVSSLKSKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+KEG+GV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPSKEGTGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK+LL FG V+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLQFGHVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAE--VLTEGPLHEE 648
            N+LKD+FPTMEMKPNPQCSN+ACLERQKEYI+AKPARDAAA+ KM AE  + TE PLH +
Sbjct: 301  NSLKDYFPTMEMKPNPQCSNAACLERQKEYIIAKPARDAAAKAKMEAEASLATEIPLHTD 360

Query: 647  NEWNISVVDDNDLTIND-TRCSGALPEGLTHELPIADEFEKLPAAESSNDA-DDLEDLKR 474
            NEWNISVVDD++    D T  +  LPEGL  ELP ADEF+K PAAE+S    DDLE+LK+
Sbjct: 361  NEWNISVVDDSEPETKDATSSADKLPEGLARELPTADEFQKFPAAETSTTTFDDLEELKK 420

Query: 473  QLEALN 456
            QLEALN
Sbjct: 421  QLEALN 426


>gb|EMJ11706.1| hypothetical protein PRUPE_ppa006127mg [Prunus persica]
          Length = 426

 Score =  636 bits (1641), Expect = e-179
 Identities = 325/426 (76%), Positives = 361/426 (84%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +L+DL+ L+ S  DPS  A ID+++ RV+ +T+LA SG + R+KVK++SAEVV
Sbjct: 1    MEVELKEMLNDLESLKRSLTDPSHHAPIDQLQLRVERLTSLASSGPVRRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIRDFSVAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQ GMTKTDAAVQTLSDINPDVVLESYTLNITTVQGF+TF SSL++KS
Sbjct: 121  ELANMNRLFFRPEQSGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFDTFMSSLKDKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F PNKEGSGV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPNKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LKYLLNFG V+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLNFGNVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEV---LTEGPLHE 651
            N+LKDFFPTMEMKPNPQCSN ACLERQKE++L KPARDAA + KM AE    +TE PLH 
Sbjct: 301  NSLKDFFPTMEMKPNPQCSNFACLERQKEFLLVKPARDAALKAKMEAEASSSVTECPLHL 360

Query: 650  ENEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQ 471
            +NEW ISVVDD++    + + + ALPEGL  ELP ADEF+K P   +    DDLEDL++Q
Sbjct: 361  DNEWEISVVDDSEPDRIEAKSADALPEGLVRELPNADEFQKSPTEAADATIDDLEDLRKQ 420

Query: 470  LEALNA 453
            LEALN+
Sbjct: 421  LEALNS 426


>ref|XP_002321704.1| thiF family protein [Populus trichocarpa] gi|222868700|gb|EEF05831.1|
            thiF family protein [Populus trichocarpa]
          Length = 428

 Score =  634 bits (1636), Expect = e-179
 Identities = 331/427 (77%), Positives = 362/427 (84%), Gaps = 4/427 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQAL-IDKMRSRVDNVTNLAKSGTMHRAKVKELSAEV 1545
            ME ELK +L+DL  L+ S  DPS  A  I K++SRV+++T LAKS  + R KV+++SAEV
Sbjct: 1    MEAELKEMLNDLDSLKQSQPDPSNLASSILKLQSRVEHLTKLAKSAPVRRTKVQDMSAEV 60

Query: 1544 VDSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDK 1365
            VDSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYDK
Sbjct: 61   VDSNPYSRLMALQRMGIVDNYERIRDFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDK 120

Query: 1364 VELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNK 1185
            VELANMNRLFFRPEQVGMTKTDAA QTLSDINPDVVLES+TLNITTVQGFETF SSLRNK
Sbjct: 121  VELANMNRLFFRPEQVGMTKTDAASQTLSDINPDVVLESFTLNITTVQGFETFMSSLRNK 180

Query: 1184 SFSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGET 1005
            SF PNKEGSGV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGET
Sbjct: 181  SFRPNKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGET 240

Query: 1004 ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLG 825
            ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK LL FGQV+PYLG
Sbjct: 241  ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKLLLQFGQVSPYLG 300

Query: 824  YNALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT--EGPLHE 651
            YN+LKDFFPTMEM+PNPQCSN+ACLERQKEY+L KPARDAA   KM AE L+  EGPLH 
Sbjct: 301  YNSLKDFFPTMEMRPNPQCSNAACLERQKEYLLVKPARDAAIIAKMEAEALSVPEGPLHT 360

Query: 650  ENEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDA-DDLEDLKR 474
            +NEWNISVVDD+     D   S ALPEGLT ELP ADEF+K PAAE +    DD+E+L++
Sbjct: 361  DNEWNISVVDDSAPERTDATSSDALPEGLTRELPAADEFQKFPAAEPATATFDDIEELRK 420

Query: 473  QLEALNA 453
            QL+ALNA
Sbjct: 421  QLDALNA 427


>ref|XP_004297542.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Fragaria
            vesca subsp. vesca]
          Length = 426

 Score =  633 bits (1632), Expect = e-178
 Identities = 320/426 (75%), Positives = 361/426 (84%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +L DL+ L+ S  DPS  A IDK++ RV+ +T+LAK+G + R+KV+++SAEVV
Sbjct: 1    MEAELKEMLSDLESLKRSLTDPSHTAPIDKLQMRVERLTSLAKTGPVRRSKVQDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVKNYERIREFSVAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQ GMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSL++KS
Sbjct: 121  ELANMNRLFFRPEQSGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLKDKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F PNKEGSGV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPNKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN+LKYLLNFG V+PYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLNFGNVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEV---LTEGPLHE 651
            N+LKDFFPTMEM+PNPQCSN AC+ERQKEY+LAKPARDAA++ KM AE    + E PLH 
Sbjct: 301  NSLKDFFPTMEMRPNPQCSNFACMERQKEYLLAKPARDAASKVKMEAEASSSVIEAPLHL 360

Query: 650  ENEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQ 471
            +NEW ISVVDD +    + +    LPEGL  ELP AD+F+K  A  +  + DDLE+L++Q
Sbjct: 361  DNEWEISVVDDTEPHSIEAKSLDTLPEGLVRELPTADDFQKPAAEAADTNVDDLEELRKQ 420

Query: 470  LEALNA 453
            L+ALN+
Sbjct: 421  LDALNS 426


>gb|ESW27544.1| hypothetical protein PHAVU_003G211200g [Phaseolus vulgaris]
          Length = 426

 Score =  632 bits (1629), Expect = e-178
 Identities = 324/425 (76%), Positives = 359/425 (84%), Gaps = 2/425 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +  DL+FL  S  DPS  AL+ K++ RVD++  LA +  + R+KV+++SAEVV
Sbjct: 1    MEIELKELNADLQFLSQSLPDPSHHALLRKIQLRVDDLAKLAAAAPVRRSKVEDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIRDFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVG+TKTDAAVQTLSDINPDVVLESYTLNITTV GFETF SSL+ KS
Sbjct: 121  ELANMNRLFFRPDQVGLTKTDAAVQTLSDINPDVVLESYTLNITTVTGFETFMSSLKKKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F PNKEGSGV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGE+A
Sbjct: 181  FRPNKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGESA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN+LK LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKLLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT--EGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN ACL+RQ+EY+LAKPARDAAA+ KM AEVL+  E PLH++
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNVACLKRQEEYMLAKPARDAAAKAKMEAEVLSTEEVPLHDD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQL 468
            NEWNISVVDD +L   DTR S  LPEGLT ELP ADEF K        + DDLEDL+RQL
Sbjct: 361  NEWNISVVDDVELDGPDTRSSDVLPEGLTRELPSADEFHKATLDSPEANNDDLEDLRRQL 420

Query: 467  EALNA 453
            EA+N+
Sbjct: 421  EAINS 425


>ref|XP_004145156.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Cucumis
            sativus] gi|449474254|ref|XP_004154119.1| PREDICTED:
            ubiquitin-like modifier-activating enzyme 5-like [Cucumis
            sativus] gi|449503682|ref|XP_004162124.1| PREDICTED:
            ubiquitin-like modifier-activating enzyme 5-like [Cucumis
            sativus]
          Length = 426

 Score =  630 bits (1625), Expect = e-178
 Identities = 325/426 (76%), Positives = 360/426 (84%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK + +DL+ L++S  DPS +  +DK++  V+ +TNL+KS  + R+KVK++SAEVV
Sbjct: 1    MEAELKDMWNDLESLKHSLTDPSLRGPVDKLQLHVERLTNLSKSVPVRRSKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQVGMTKTDAAVQTL+DINPDVVLESYT NITTVQGFETF SSLRNKS
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTFNITTVQGFETFMSSLRNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            FSP KEGSGV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN+LK+LL FG V+PYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT--EGPLHEE 648
            N+LKD+FPTMEM+PNPQCSN ACLERQKE+I+AKPARDAAA  KM AE LT  E PLH +
Sbjct: 301  NSLKDYFPTMEMRPNPQCSNVACLERQKEFIIAKPARDAAAMAKMEAEALTVEEIPLHAD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAE-SSNDADDLEDLKRQ 471
            NEWNISVVD+ ++       S ALPEGL  ELP ADE +  P AE ++   DDLEDLKRQ
Sbjct: 361  NEWNISVVDEVEIETVGATSSDALPEGLVRELPNADESQHPPPAEPAAPSLDDLEDLKRQ 420

Query: 470  LEALNA 453
            LEALN+
Sbjct: 421  LEALNS 426


>ref|XP_006485349.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Citrus
            sinensis]
          Length = 427

 Score =  627 bits (1617), Expect = e-177
 Identities = 325/426 (76%), Positives = 359/426 (84%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK ++DDL+ L+ S  D S  A I+K+  RV+N+T +A S T  R+KVK++S+EVV
Sbjct: 1    MEAELKDMIDDLESLKRSLPDSSLHASINKLELRVENLTKMAMSATARRSKVKDMSSEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRPEQVGMTKTDAAVQTL+DINPDVVLES+TLNITTVQGFETF SSLRNKS
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESFTLNITTVQGFETFMSSLRNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+KEGSGV LVLSCVDNYEARM VNQACNELNQTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPSKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LKYLL FGQV+ YLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLKFGQVSSYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEV--LTEGPLHEE 648
            N+LKD+FPTM MKPNPQCSN+ACLERQKE ILAKPARDAAA+ KM AE    TE  LH +
Sbjct: 301  NSLKDYFPTMAMKPNPQCSNAACLERQKECILAKPARDAAAKAKMEAEASSATECSLHAD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPA-AESSNDADDLEDLKRQ 471
            NEWNISVVDD++    +   + ALPEGLTHELP AD+F+K PA   +    DDL+ L++Q
Sbjct: 361  NEWNISVVDDSNPDSVEATSADALPEGLTHELPNADDFKKSPAPGPTDTSIDDLDLLRKQ 420

Query: 470  LEALNA 453
            LEALNA
Sbjct: 421  LEALNA 426


>ref|XP_003549854.2| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Glycine
            max]
          Length = 426

 Score =  626 bits (1615), Expect = e-176
 Identities = 323/425 (76%), Positives = 355/425 (83%), Gaps = 2/425 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME  LK +  DL+ L  S  DPS   L+ K++ RV+++  LA++  + R+KV+++SAEVV
Sbjct: 1    MEVVLKELHADLQSLSQSLPDPSHHDLLRKIQLRVEDLAKLAEAAPVRRSKVEDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVDNYERIRDFSVAIVGVGGVGSVSAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVGMTKTDAAVQTLSDINPDVVLESYTLNITTV GFETF SSL+NKS
Sbjct: 121  ELANMNRLFFRPDQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVTGFETFMSSLKNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
              PNK+GSGV LVLSCVDNYEARM VNQACNEL+QTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  LRPNKQGSGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKLLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEV--LTEGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN ACLERQ+EY+LAKPARDAAA+ KM AE     E PLHEE
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNVACLERQEEYMLAKPARDAAAKAKMEAEAPSTEEVPLHEE 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQL 468
            NEWNISVVDD +L   DTR S  LPEGLTHELP ADEF K        D DDLEDL+RQL
Sbjct: 361  NEWNISVVDDIELDSPDTRSSDVLPEGLTHELPTADEFHKATLDAPLVDNDDLEDLRRQL 420

Query: 467  EALNA 453
            EA+N+
Sbjct: 421  EAINS 425


>ref|XP_004501700.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Cicer
            arietinum]
          Length = 427

 Score =  626 bits (1615), Expect = e-176
 Identities = 318/426 (74%), Positives = 363/426 (85%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME EL  ++ DL+ L  S  DPS    I K++SRV+++ +L K+G + R+KV+++SAEVV
Sbjct: 1    MEIELNDLIADLQSLNQSLPDPSLSDAIHKIQSRVEHIADLVKAGPVRRSKVQDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIRDFSIAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+Q GMTKTDAAVQTLSDINPDVVLES+TLNITTV GFETF SSL+NKS
Sbjct: 121  ELANMNRLFFRPDQAGMTKTDAAVQTLSDINPDVVLESFTLNITTVDGFETFMSSLKNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+KEGSGV LVLSCVDNYEARM VNQACNEL+QTW+ESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPDKEGSGVDLVLSCVDNYEARMAVNQACNELSQTWLESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN+LK+LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAE--VLTEGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN+ACL+RQ+EYILAKPARDAAA+ KM AE   + EGPLH++
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNAACLKRQEEYILAKPARDAAAKAKMEAEGPSIEEGPLHDD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAES-SNDADDLEDLKRQ 471
            NEWNISVVDD +    +   S ALPEGLTHELP ADEF+     E+ +ND DDL+DL++Q
Sbjct: 361  NEWNISVVDDCEPDGPNPSSSDALPEGLTHELPTADEFQNESTDETPTNDNDDLDDLRKQ 420

Query: 470  LEALNA 453
            LEA+N+
Sbjct: 421  LEAINS 426


>ref|XP_003525714.2| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Glycine
            max]
          Length = 426

 Score =  626 bits (1614), Expect = e-176
 Identities = 322/425 (75%), Positives = 354/425 (83%), Gaps = 2/425 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +  DL+ L  S  DPS   L+ K++ RV ++  LA++  + R+KV+++SAEVV
Sbjct: 1    MEVELKELHADLQSLSQSLPDPSHHDLLRKIQLRVGDLAKLAEAAPVRRSKVEDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVDNYERIRDFSVAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVGMTKTDAAVQTLSDINPDV LESYTLNITTV GFETF SSL+NKS
Sbjct: 121  ELANMNRLFFRPDQVGMTKTDAAVQTLSDINPDVALESYTLNITTVTGFETFMSSLKNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F PNK+GSGV LVLSCVDNYEARM VNQACNEL+QTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPNKQGSGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKLLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEV--LTEGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN ACLERQ+EY+ AKPARDAAA+ KM AE     E PLHE+
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNVACLERQEEYLFAKPARDAAAKAKMEAEAPSTEEVPLHED 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQL 468
            NEWNISVVDD +L   DTR S  LPEGLTHELP ADEF K        D DDLEDL+RQL
Sbjct: 361  NEWNISVVDDVELVGPDTRSSDVLPEGLTHELPTADEFHKATLDAPVVDNDDLEDLRRQL 420

Query: 467  EALNA 453
            EA+N+
Sbjct: 421  EAINS 425


>ref|XP_006418036.1| hypothetical protein EUTSA_v10007699mg [Eutrema salsugineum]
            gi|557095807|gb|ESQ36389.1| hypothetical protein
            EUTSA_v10007699mg [Eutrema salsugineum]
          Length = 428

 Score =  626 bits (1614), Expect = e-176
 Identities = 322/430 (74%), Positives = 362/430 (84%), Gaps = 7/430 (1%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME E K +LDDL  L+ S  DP   A I K+RS V+N+  L+K     R+KVKELS+EVV
Sbjct: 1    MEVEFKAMLDDLDVLEKSLSDP---APIHKLRSHVENLATLSKCNPHRRSKVKELSSEVV 57

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYD V
Sbjct: 58   DSNPYSRLMALQRMGIVDNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTV 117

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVGMTKTDAAVQTL++INPDVVLES+T+NITTVQGFETFTSSL+NKS
Sbjct: 118  ELANMNRLFFRPDQVGMTKTDAAVQTLAEINPDVVLESFTMNITTVQGFETFTSSLKNKS 177

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+KEG+GV LVLSCVDNYEARM VNQACNEL+QTWMESGVSE+AVSGHIQLL+PGETA
Sbjct: 178  FCPSKEGTGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLVPGETA 237

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQNSLK+LLNFG+V+PYLGY
Sbjct: 238  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNSLKFLLNFGEVSPYLGY 297

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT---EGPLHE 651
            N+LKDFFPTM+M+PNPQCSN ACLERQKEY+LAKPARDAAA+ KM A+ LT   +GPLH+
Sbjct: 298  NSLKDFFPTMQMRPNPQCSNVACLERQKEYMLAKPARDAAAKAKMEADALTAIDDGPLHD 357

Query: 650  ENEWNISVVDDNDL--TINDTRCSGALPEGLTHELPIADEFEKLPA--AESSNDADDLED 483
            +NEWNISVVDD +   T   +  S  LPEGLT ELP+ADE+EK  A     + + DDLED
Sbjct: 358  DNEWNISVVDDENEKDTTKASSSSDTLPEGLTRELPVADEYEKAIAIGPGETEEEDDLED 417

Query: 482  LKRQLEALNA 453
            LK+QLEALNA
Sbjct: 418  LKKQLEALNA 427


>ref|XP_003602810.1| Ubiquitin-like modifier-activating enzyme [Medicago truncatula]
            gi|355491858|gb|AES73061.1| Ubiquitin-like
            modifier-activating enzyme [Medicago truncatula]
            gi|388517059|gb|AFK46591.1| unknown [Medicago truncatula]
          Length = 427

 Score =  622 bits (1604), Expect = e-175
 Identities = 313/426 (73%), Positives = 362/426 (84%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME EL  ++ DL+ L  S  DPS +    K++S V+++ +L ++  + R KVK++SAEVV
Sbjct: 1    MEIELNDLITDLQSLNQSLPDPSLRDAFHKIQSHVEHIADLVRAQPVRRTKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIRDFSIAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+Q GMTKTDAA+QTLSDINPDVVLES+TLNITTV GFETF SSL+NKS
Sbjct: 121  ELANMNRLFFRPDQAGMTKTDAALQTLSDINPDVVLESFTLNITTVDGFETFMSSLKNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+K+GSGV LVLSCVDNYEARM VNQACNEL+QTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPDKQGSGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGV+AGLLVQN+LK+LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVIAGLLVQNTLKFLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAE--VLTEGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN+ACL+RQ EYILAKP RDAAA+ K+ AE  ++ EGPLH++
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNAACLKRQGEYILAKPTRDAAAKAKLEAEGPLIEEGPLHDD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAES-SNDADDLEDLKRQ 471
            NEWNISVVDD +    +T+ S ALPEGLTHELP ADEF+ L   E+ SND DDL++L++Q
Sbjct: 361  NEWNISVVDDCEPDGANTKSSDALPEGLTHELPTADEFQNLVTREAPSNDNDDLDELRKQ 420

Query: 470  LEALNA 453
            LEA+N+
Sbjct: 421  LEAINS 426


>ref|NP_172027.2| NAD(P)-binding Rossmann-fold superfamily protein [Arabidopsis
            thaliana] gi|290463407|sp|O23034.2|UBA5_ARATH RecName:
            Full=Ubiquitin-like modifier-activating enzyme 5;
            Short=Ubiquitin-activating enzyme 5
            gi|332189707|gb|AEE27828.1| NAD(P)-binding Rossmann-fold
            superfamily protein [Arabidopsis thaliana]
          Length = 431

 Score =  619 bits (1597), Expect = e-174
 Identities = 323/433 (74%), Positives = 359/433 (82%), Gaps = 10/433 (2%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME   K +LDDL  L+ S  DP   ALI+K+RS V+N+  L+K     R+KVKELS+EVV
Sbjct: 1    MEVGFKALLDDLDVLEKSLSDP---ALINKLRSHVENLATLSKCNPHRRSKVKELSSEVV 57

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYD V
Sbjct: 58   DSNPYSRLMALQRMGIVDNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTV 117

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVGMTKTDAAVQTL++INPDVVLES+T+NITTVQGFETFTSSL NKS
Sbjct: 118  ELANMNRLFFRPDQVGMTKTDAAVQTLAEINPDVVLESFTMNITTVQGFETFTSSLTNKS 177

Query: 1181 FSPNKEG-SGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGET 1005
            F P+KEG SGV LVLSCVDNYEARM VNQACNEL QTWMESGVSE+AVSGHIQLL+PGET
Sbjct: 178  FCPSKEGGSGVDLVLSCVDNYEARMAVNQACNELRQTWMESGVSEDAVSGHIQLLVPGET 237

Query: 1004 ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLG 825
            ACFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQNSLK+LLNFG+V+PYLG
Sbjct: 238  ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNSLKFLLNFGEVSPYLG 297

Query: 824  YNALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEVLT---EGPLH 654
            YN+LKDFFPTM+M+PNPQCSN ACLERQKEY+LAKP RDAAA+ KM A+  T   EGPLH
Sbjct: 298  YNSLKDFFPTMKMRPNPQCSNVACLERQKEYMLAKPERDAAAKAKMEADASTTIDEGPLH 357

Query: 653  EENEWNISVVDDNDL--TINDTRCSGALPEGLTHELPIADEFEKLPAAES----SNDADD 492
            ++NEWNISVVDD +   T      S  LPEGLT ELP+ADE+EK  A  S    + + DD
Sbjct: 358  DDNEWNISVVDDENEKDTTKAASSSDTLPEGLTRELPVADEYEKAIAIASGSGETEEEDD 417

Query: 491  LEDLKRQLEALNA 453
            LEDLK+QLEALNA
Sbjct: 418  LEDLKKQLEALNA 430


>ref|XP_003595421.1| Ubiquitin-like modifier-activating enzyme [Medicago truncatula]
            gi|355484469|gb|AES65672.1| Ubiquitin-like
            modifier-activating enzyme [Medicago truncatula]
          Length = 427

 Score =  619 bits (1595), Expect = e-174
 Identities = 312/426 (73%), Positives = 361/426 (84%), Gaps = 3/426 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME EL  ++ DL+ L  S  DPS +    K++S V+++ +L +S  + R KVK++SAEVV
Sbjct: 1    MEIELNDLITDLQSLNQSLPDPSFRDAFLKIQSHVEHIADLVRSQPVRRTKVKDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            D+NPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DNNPYSRLMALQRMGIVENYERIRDFSIAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+Q GMTKTDAA+QTLSDINPDVVLES+TLNITTV GFETF SSL+NKS
Sbjct: 121  ELANMNRLFFRPDQAGMTKTDAALQTLSDINPDVVLESFTLNITTVDGFETFMSSLKNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F P+K+GSGV LVLSCVDNYEARM VNQACNEL+QTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPDKQGSGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGV+AGLLVQN+LK+LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVIAGLLVQNTLKFLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAE--VLTEGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN+ACL+RQ EYILAKP RDAAA+ K+ AE  ++ EGPLH++
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNAACLKRQGEYILAKPTRDAAAKAKLEAEGPLIEEGPLHDD 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAES-SNDADDLEDLKRQ 471
            NEWNISVVDD +    +T+ S ALPEGLTHELP ADEF+ L   E+  ND DDL++L++Q
Sbjct: 361  NEWNISVVDDCEPDGANTKSSDALPEGLTHELPTADEFQNLVTREAPRNDNDDLDELRKQ 420

Query: 470  LEALNA 453
            LEA+N+
Sbjct: 421  LEAINS 426


>gb|ACU21220.1| unknown [Glycine max]
          Length = 456

 Score =  616 bits (1589), Expect = e-173
 Identities = 318/423 (75%), Positives = 349/423 (82%), Gaps = 2/423 (0%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK +  DL+ L  S  DPS   L+ K++ RV ++  LA++  + R+KV+++SAEVV
Sbjct: 1    MEVELKELHADLQSLSQSLPDPSHHDLLRKIQLRVGDLAKLAEAAPVRRSKVEDMSAEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIV+NYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVDNYERIRDFSVAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFTSSLRNKS 1182
            ELANMNRLFFRP+QVGMTKTDAAVQTLSDINPDV LESYTLNITTV GFETF SSL+NKS
Sbjct: 121  ELANMNRLFFRPDQVGMTKTDAAVQTLSDINPDVALESYTLNITTVTGFETFMSSLKNKS 180

Query: 1181 FSPNKEGSGVALVLSCVDNYEARMVVNQACNELNQTWMESGVSENAVSGHIQLLIPGETA 1002
            F PNK+GSGV LVLSCVDNYEARM VNQACNEL+QTWMESGVSE+AVSGHIQLLIPGETA
Sbjct: 181  FRPNKQGSGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 1001 CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGY 822
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQN+LK LL FGQV+PYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKLLLGFGQVSPYLGY 300

Query: 821  NALKDFFPTMEMKPNPQCSNSACLERQKEYILAKPARDAAAREKMSAEV--LTEGPLHEE 648
            N+LKDFFPTM+MKPNPQCSN ACLERQ+EY+ AKPARDAAA+ KM AE     E PLHE+
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNVACLERQEEYLFAKPARDAAAKAKMEAEAPSTEEVPLHED 360

Query: 647  NEWNISVVDDNDLTINDTRCSGALPEGLTHELPIADEFEKLPAAESSNDADDLEDLKRQL 468
            NEWNISVVDD +L   DTR S  LPEGLTHELP ADEF K        D DDLEDL+  L
Sbjct: 361  NEWNISVVDDVELVGPDTRSSDVLPEGLTHELPTADEFHKATLDAPVVDNDDLEDLRSNL 420

Query: 467  EAL 459
            + L
Sbjct: 421  KPL 423


>ref|XP_006436836.1| hypothetical protein CICLE_v10031478mg [Citrus clementina]
            gi|557539032|gb|ESR50076.1| hypothetical protein
            CICLE_v10031478mg [Citrus clementina]
          Length = 460

 Score =  610 bits (1573), Expect = e-172
 Identities = 325/459 (70%), Positives = 359/459 (78%), Gaps = 36/459 (7%)
 Frame = -3

Query: 1721 METELKGILDDLKFLQNSFLDPSQQALIDKMRSRVDNVTNLAKSGTMHRAKVKELSAEVV 1542
            ME ELK ++DDL+ L+ S  D S  A I+K+  RV+N+T +A S T  R+KVK++S+EVV
Sbjct: 1    MEAELKDMIDDLESLKRSLPDSSLHASINKLELRVENLTKMAMSATARRSKVKDMSSEVV 60

Query: 1541 DSNPYSRLMALQRMGIVENYERIRHFXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1362
            DSNPYSRLMALQRMGIVENYERIR F              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGVGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1361 ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLE----------------------- 1251
            ELANMNRLFFRPEQVGMTKTDAAVQTL+DINPDVVLE                       
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLEEINVWTISFNILRQKLLGGERRA 180

Query: 1250 ----------SYTLNITTVQGFETFTSSLRNKSFSPNKEGSGVALVLSCVDNYEARMVVN 1101
                      S+TLNITTVQGFETF SSLRNKSF P+KEGSGV LVLSCVDNYEARM VN
Sbjct: 181  YKCTRRYRFSSFTLNITTVQGFETFMSSLRNKSFRPSKEGSGVDLVLSCVDNYEARMAVN 240

Query: 1100 QACNELNQTWMESGVSENAVSGHIQLLIPGETACFACAPPLVVASGVDERTLKREGVCAA 921
            QACNELNQTWMESGVSE+AVSGHIQLLIPGETACFACAPPLVVASGVDERTLKREGVCAA
Sbjct: 241  QACNELNQTWMESGVSEDAVSGHIQLLIPGETACFACAPPLVVASGVDERTLKREGVCAA 300

Query: 920  SLPTTMGVVAGLLVQNSLKYLLNFGQVTPYLGYNALKDFFPTMEMKPNPQCSNSACLERQ 741
            SLPTTMGVVAGLLVQN+LKYLL FGQV+ YLGYN+LKD+FPTM MKPNPQCSN+ACLERQ
Sbjct: 301  SLPTTMGVVAGLLVQNTLKYLLKFGQVSSYLGYNSLKDYFPTMAMKPNPQCSNAACLERQ 360

Query: 740  KEYILAKPARDAAAREKMSAEV--LTEGPLHEENEWNISVVDDNDLTINDTRCSGALPEG 567
            KE ILAKPARDAAA+ KM AE    TE  LH +NEWNISVVDD++    +   + ALPEG
Sbjct: 361  KECILAKPARDAAAKAKMEAEASSATECSLHADNEWNISVVDDSNPDSVEATSADALPEG 420

Query: 566  LTHELPIADEFEKLPA-AESSNDADDLEDLKRQLEALNA 453
            LTHELP AD+F+K PA   +    DDL+ L++QLEALNA
Sbjct: 421  LTHELPNADDFKKSPAPGPTDTSIDDLDLLRKQLEALNA 459