BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Catharanthus22_contig00006710
         (470 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004252037.1| PREDICTED: putative late blight resistance p...    89   6e-16
ref|XP_006349899.1| PREDICTED: putative late blight resistance p...    88   1e-15
ref|XP_006348816.1| PREDICTED: putative late blight resistance p...    87   2e-15
ref|XP_006349898.1| PREDICTED: putative late blight resistance p...    87   3e-15
ref|XP_004233876.1| PREDICTED: late blight resistance protein R1...    85   1e-14
ref|XP_006351974.1| PREDICTED: putative late blight resistance p...    84   3e-14
ref|XP_006366838.1| PREDICTED: putative late blight resistance p...    82   7e-14
ref|XP_006362840.1| PREDICTED: putative late blight resistance p...    81   1e-13
ref|XP_006345448.1| PREDICTED: putative late blight resistance p...    81   2e-13
gb|AAT39957.1| Putative late blight resistance protein, identica...    81   2e-13
ref|XP_004231117.1| PREDICTED: putative late blight resistance p...    80   2e-13
ref|XP_004243044.1| PREDICTED: putative late blight resistance p...    80   3e-13
ref|XP_004239389.1| PREDICTED: late blight resistance protein R1...    80   3e-13
ref|XP_006350138.1| PREDICTED: putative late blight resistance p...    80   4e-13
ref|XP_004237146.1| PREDICTED: putative late blight resistance p...    80   4e-13
ref|XP_006356446.1| PREDICTED: putative late blight resistance p...    79   6e-13
ref|XP_004237148.1| PREDICTED: putative late blight resistance p...    79   8e-13
ref|XP_006350137.1| PREDICTED: putative late blight resistance p...    78   1e-12
ref|XP_004239472.1| PREDICTED: putative late blight resistance p...    78   1e-12
ref|XP_006363255.1| PREDICTED: putative late blight resistance p...    78   1e-12

>ref|XP_004252037.1| PREDICTED: putative late blight resistance protein homolog
            R1B-16-like [Solanum lycopersicum]
          Length = 934

 Score = 89.0 bits (219), Expect = 6e-16
 Identities = 43/107 (40%), Positives = 64/107 (59%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFY 180
            NLE+LKL    F   +W+   E F +LK LQ+    ++ W+   D FP L+ LVL CC +
Sbjct: 813  NLEVLKLKPYAFTGTKWEQVEEGFCSLKLLQIEMFDLEHWSASNDHFPVLEHLVLKCCLH 872

Query: 181  LKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQV 321
            L  IP  LG+I +L +IE+ N S+ A  SA EI  E+ ++G++  +V
Sbjct: 873  LDSIPHDLGDIPTLQIIELENSSQSAVLSAKEIQEEQQNIGNETLEV 919


>ref|XP_006349899.1| PREDICTED: putative late blight resistance protein homolog
           R1A-10-like [Solanum tuberosum]
          Length = 413

 Score = 87.8 bits (216), Expect = 1e-15
 Identities = 48/118 (40%), Positives = 72/118 (61%), Gaps = 3/118 (2%)
 Frame = +1

Query: 1   NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSR--IQEWNTCTDPFPCLQQLVLDCC 174
           NL++L L  I FE  +W++ ++EF  L+ L+L   +   +EW    D FP L+ LVL  C
Sbjct: 289 NLQVLSLKGISFETCKWEVRDDEFPQLRVLKLHAGKAHFEEWFVADDAFPNLEHLVLRDC 348

Query: 175 FYLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERL-DLGDDGFQVLVSSE*RK 345
            +LKEIPS  GEISSL  I VRNC++  ++SA +I   ++ D  +  F+V ++ E  K
Sbjct: 349 KFLKEIPSWFGEISSLKSIAVRNCNENVDKSASDIRETQVEDYQNSKFEVFITRENNK 406


>ref|XP_006348816.1| PREDICTED: putative late blight resistance protein homolog
            R1B-23-like [Solanum tuberosum]
          Length = 1437

 Score = 87.4 bits (215), Expect = 2e-15
 Identities = 45/110 (40%), Positives = 64/110 (58%), Gaps = 1/110 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFY 180
            NLE+LKL  +      W +++E+F  LKFL+L      EW+   D FPCL+ LVL  C Y
Sbjct: 1310 NLEVLKLLGVTISSNTWKVKDEQFSKLKFLKLENLSFSEWDVSDDAFPCLEHLVLKRCRY 1369

Query: 181  LKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERL-DLGDDGFQVLV 327
            L+EIPS  G + SL  IEV +C +    SA  I   ++ D+G   F+V++
Sbjct: 1370 LEEIPSCFGYMPSLKSIEVESCKESLADSAMVIKEMQVEDMGFSDFEVII 1419


>ref|XP_006349898.1| PREDICTED: putative late blight resistance protein homolog
           R1A-10-like [Solanum tuberosum]
          Length = 386

 Score = 86.7 bits (213), Expect = 3e-15
 Identities = 45/112 (40%), Positives = 70/112 (62%), Gaps = 3/112 (2%)
 Frame = +1

Query: 1   NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSR--IQEWNTCTDPFPCLQQLVLDCC 174
           NL++LKL  I FE  +W++ + EF  L+ L+L   +   +EW    D F  L++LVL  C
Sbjct: 258 NLQVLKLKGISFETCKWEVRDGEFPQLRALKLHAGQAHFEEWTVAEDSFANLERLVLSDC 317

Query: 175 FYLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERL-DLGDDGFQVLV 327
            +LKEIPS  G+ISSL  IEVRNC++  ++SA +I   ++ D  +  F++ +
Sbjct: 318 VFLKEIPSCFGDISSLKSIEVRNCNENVDKSARDIRETQVEDYQNSEFEIFI 369


>ref|XP_004233876.1| PREDICTED: late blight resistance protein R1-A-like [Solanum
           lycopersicum]
          Length = 733

 Score = 84.7 bits (208), Expect = 1e-14
 Identities = 47/110 (42%), Positives = 67/110 (60%), Gaps = 1/110 (0%)
 Frame = +1

Query: 1   NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFY 180
           NLE+L +    F  ++W +    FLNL+ L+L  S I+ WN   D FPCL+QLVL  C  
Sbjct: 621 NLEVLNILLSAFTGKKWTVGVGGFLNLRLLKLEHSSIKHWNMFVDSFPCLEQLVLRWCGK 680

Query: 181 LKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILN-ERLDLGDDGFQVLV 327
           L+EIP+S G + SL  IE R C   A +SA +I N +R D+ +  F+V++
Sbjct: 681 LEEIPASFGSMPSLQKIEARACCPSARKSAVKIRNMQRDDMKNLDFKVIM 730


>ref|XP_006351974.1| PREDICTED: putative late blight resistance protein homolog
            R1B-16-like [Solanum tuberosum]
          Length = 926

 Score = 83.6 bits (205), Expect = 3e-14
 Identities = 43/107 (40%), Positives = 62/107 (57%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFY 180
            NLE+LKL    F   +W+   E F +LK L +  S I+ W+   D FP L+ LVL  C +
Sbjct: 805  NLEVLKLKNYAFTGPKWEQVEEGFGSLKLLLIEISDIKHWSASNDHFPVLEHLVLKSCLH 864

Query: 181  LKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQV 321
            L  IP  LG+I +L +IE+ N S+ A  SA EI  E+  +G++  +V
Sbjct: 865  LDSIPHDLGDIPTLQIIELENSSQSAVLSAKEIQEEQQSMGNETLEV 911


>ref|XP_006366838.1| PREDICTED: putative late blight resistance protein homolog
            R1B-16-like [Solanum tuberosum]
          Length = 889

 Score = 82.0 bits (201), Expect = 7e-14
 Identities = 43/110 (39%), Positives = 64/110 (58%), Gaps = 1/110 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLN-LKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NL++LK+ +  F+   W + +EE  N LKFL +  + ++ W   +  FP LQ LVL  C 
Sbjct: 772  NLQVLKIKDNGFDGDAWRLSDEEIFNQLKFLLIDRTNLKRWKAGSVNFPKLQHLVLKRCI 831

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQVLV 327
            YL+EIP  +GEI +L  IE+ NC   A +S  EI  E+  +G+D   V +
Sbjct: 832  YLEEIPKDIGEIYTLESIELLNCRTSAAKSVKEIQEEQESMGNDCLSVRI 881


>ref|XP_006362840.1| PREDICTED: putative late blight resistance protein homolog
            R1B-17-like [Solanum tuberosum]
          Length = 876

 Score = 81.3 bits (199), Expect = 1e-13
 Identities = 44/112 (39%), Positives = 66/112 (58%), Gaps = 3/112 (2%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEW---NTCTDPFPCLQQLVLDC 171
            NLE+LKL +  F+   W+ + E F  LK+L +    +  W   +T + PFP LQ LVL  
Sbjct: 761  NLEVLKLKDFSFQGPEWETDEEGFHRLKYLLVESRDLVVWKQASTDSYPFPVLQHLVLRF 820

Query: 172  CFYLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQVLV 327
            C+ LKEIP  +G+I SL +I++ +CS  A R A  I  E++D G+   +V +
Sbjct: 821  CYKLKEIPYEIGDIPSLQVIKLHSCSPYATRLARMIEKEQIDSGNSCLEVFI 872


>ref|XP_006345448.1| PREDICTED: putative late blight resistance protein homolog
            R1A-10-like [Solanum tuberosum]
          Length = 888

 Score = 80.9 bits (198), Expect = 2e-13
 Identities = 48/113 (42%), Positives = 66/113 (58%), Gaps = 1/113 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENE-EFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NLE+LKL +  FE   W + +E EF  LKFL L   R+  W   +  FP LQ+LVL  C 
Sbjct: 756  NLEVLKLRDNEFEGCAWTLSDEDEFSQLKFLLLSEPRLVNWEAGSVNFPNLQKLVLRKCI 815

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQVLVSSE 336
             L+EIP  +GEI +L MIE+  CS  A+ SA EI  E+  +G+    + V ++
Sbjct: 816  RLEEIPLDIGEICTLEMIELICCSSSAQNSANEIREEQESMGNSCLDIRVYAD 868


>gb|AAT39957.1| Putative late blight resistance protein, identical [Solanum demissum]
            gi|49533774|gb|AAT66773.1| Putative late blight
            resistance protein, identical [Solanum demissum]
          Length = 1268

 Score = 80.9 bits (198), Expect = 2e-13
 Identities = 45/113 (39%), Positives = 65/113 (57%), Gaps = 1/113 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQL-RCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NL +LKL  I+F+  +W++ + EF  L  L+L +C    EW T    FP L+ LVL  C 
Sbjct: 1090 NLCVLKLVSIFFDNDKWEVRDGEFFGLTVLKLVKCEFFDEWKTSDFAFPMLEHLVLRECP 1149

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQVLVSSE 336
            YLKEIP S   I +L+ I+V++CS+  ERSA  I   +   G +  +V +  E
Sbjct: 1150 YLKEIPLSFEAIPTLNSIKVKSCSESVERSATIIKEVQEAYGSNDVRVFIQRE 1202


>ref|XP_004231117.1| PREDICTED: putative late blight resistance protein homolog
            R1A-10-like [Solanum lycopersicum]
          Length = 880

 Score = 80.5 bits (197), Expect = 2e-13
 Identities = 48/110 (43%), Positives = 64/110 (58%), Gaps = 1/110 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENE-EFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NLE+LKL +  FE   W + +E EF  LKFL L   R+  W   +  FP LQ+LVL  C 
Sbjct: 752  NLEVLKLRDNEFEGCAWTLSDEDEFSQLKFLLLAEPRLLNWEAGSVNFPNLQKLVLRKCI 811

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQVLV 327
             L+EIP  +GEI +L MIE+  CS  A+ SA EI  E+  +G+    + V
Sbjct: 812  CLEEIPIDIGEICTLEMIELICCSSSAQNSANEIREEQESMGNSCLDIRV 861


>ref|XP_004243044.1| PREDICTED: putative late blight resistance protein homolog
            R1A-10-like [Solanum lycopersicum]
          Length = 902

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 40/110 (36%), Positives = 65/110 (59%), Gaps = 1/110 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLN-LKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NL++LK+ +  F+   W + +E+  N LK+L +  + ++ W   +  FP LQ+LVL  C 
Sbjct: 772  NLQVLKIKDNGFDGDAWRLSDEQIFNQLKYLLIDRTNVKSWKAGSVNFPKLQRLVLKRCI 831

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGDDGFQVLV 327
            YL+EIP  +GE+ +L  IE+ NC   A +S  EI  E+  +G+D   V +
Sbjct: 832  YLEEIPKDIGEVYTLESIELLNCRTSAAKSVKEIQEEQESMGNDCLSVRI 881


>ref|XP_004239389.1| PREDICTED: late blight resistance protein R1-A-like [Solanum
            lycopersicum]
          Length = 1237

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 43/115 (37%), Positives = 67/115 (58%), Gaps = 2/115 (1%)
 Frame = +1

Query: 10   ILKLSEIWFEERRWDMENEEFLNLKFLQL-RCSRIQEWNTCTDPFPCLQQLVLDCCFYLK 186
            +LKL  ++F+   W++ + EF  L+ L+L +C    EW      FP L+ LVL  C YL 
Sbjct: 1094 VLKLVSVFFDNDEWEVRDGEFSGLRVLKLVKCEFFDEWKASDHAFPILEHLVLRECPYLM 1153

Query: 187  EIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDL-GDDGFQVLVSSE*RKE 348
            EIPSS  +I +L+ I+V++CS+  E+SA ++  E  D+ G D   V +  E  K+
Sbjct: 1154 EIPSSFQDIPTLNSIKVKSCSESVEQSAIKMKRELEDIYGSDHLMVFIQRELNKD 1208


>ref|XP_006350138.1| PREDICTED: putative late blight resistance protein homolog
            R1C-3-like, partial [Solanum tuberosum]
          Length = 911

 Score = 79.7 bits (195), Expect = 4e-13
 Identities = 39/98 (39%), Positives = 59/98 (60%)
 Frame = +1

Query: 4    LEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFYL 183
            LE+L+L++     R W++  E F  LKFL L+ S IQ W    D FPCL++L LD C +L
Sbjct: 786  LELLELTQGVCRGREWEVAEEGFPFLKFLLLKRSHIQYWRASCDHFPCLERLFLDSCRFL 845

Query: 184  KEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLD 297
              IP    +I++L++I++RNC +    SA +I  +  D
Sbjct: 846  DSIPQDFADITTLALIDIRNCPESVGNSAKQIQQDIQD 883


>ref|XP_004237146.1| PREDICTED: putative late blight resistance protein homolog
            R1A-10-like [Solanum lycopersicum]
          Length = 840

 Score = 79.7 bits (195), Expect = 4e-13
 Identities = 43/103 (41%), Positives = 65/103 (63%), Gaps = 1/103 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEE-FLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NLE LKL +     + W + +E+ F +LK L L  S ++ W   +D FP L++LVL  C+
Sbjct: 711  NLEELKLKQCGDMLKTWRLSDEDKFKSLKLLLLSGSSLKHWEATSDNFPNLKRLVLKNCY 770

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGD 306
             L+EIP+  GEI +L  IE+ NCS  A+ SA EI+ E+ ++G+
Sbjct: 771  RLQEIPADFGEIGTLESIELHNCSTTADDSAREIVQEQEEMGN 813


>ref|XP_006356446.1| PREDICTED: putative late blight resistance protein homolog
            R1B-14-like [Solanum tuberosum]
          Length = 1645

 Score = 79.0 bits (193), Expect = 6e-13
 Identities = 46/110 (41%), Positives = 64/110 (58%), Gaps = 1/110 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFY 180
            NL+ILKL    FE   W++++ EF  LK+L+L    I +W+   D FP L++LVL  C  
Sbjct: 1525 NLKILKLLLRTFEGDEWEVKDSEFRELKYLELENLNIAQWSVSEDAFPILERLVLTKCKR 1584

Query: 181  LKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLD-LGDDGFQVLV 327
            LK+IPS   +  SL  IEV  CS G   SA EI   + D + +D F+V +
Sbjct: 1585 LKKIPSHFDDAVSLKSIEVNWCSLGVANSAKEIQAFQHDEIANDAFKVTI 1634


>ref|XP_004237148.1| PREDICTED: putative late blight resistance protein homolog
            R1A-10-like [Solanum lycopersicum]
          Length = 881

 Score = 78.6 bits (192), Expect = 8e-13
 Identities = 44/103 (42%), Positives = 63/103 (61%), Gaps = 1/103 (0%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEE-FLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NLE LKL         W + +E+ F +LK L L  S ++ W   +D FP L++LVL  C+
Sbjct: 767  NLEELKLKNCQTLIDEWRLSDEDKFKSLKLLLLSGSSLKRWEANSDNFPNLKRLVLKNCY 826

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLDLGD 306
             L+EIP+  GEI +L  IE+ NCS  A+ SA EI+ E+ D+G+
Sbjct: 827  RLQEIPADFGEIGTLESIELHNCSTTADDSAREIVQEQEDMGN 869


>ref|XP_006350137.1| PREDICTED: putative late blight resistance protein homolog
            R1C-3-like, partial [Solanum tuberosum]
          Length = 911

 Score = 78.2 bits (191), Expect = 1e-12
 Identities = 39/98 (39%), Positives = 58/98 (59%)
 Frame = +1

Query: 4    LEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFYL 183
            LE+L+L++     R W++  E F  LKFL L+ S IQ W    D FPCL++L LD C +L
Sbjct: 786  LELLELTQGVCRGREWEVAEEGFPFLKFLLLKRSHIQYWRASCDHFPCLERLFLDSCRFL 845

Query: 184  KEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLD 297
              IP    +I++L++I +RNC +    SA +I  +  D
Sbjct: 846  DSIPQDFADITTLALIYIRNCPESVGNSAKQIQQDIQD 883


>ref|XP_004239472.1| PREDICTED: putative late blight resistance protein homolog
           R1B-16-like [Solanum lycopersicum]
          Length = 196

 Score = 78.2 bits (191), Expect = 1e-12
 Identities = 43/109 (39%), Positives = 63/109 (57%), Gaps = 1/109 (0%)
 Frame = +1

Query: 4   LEILKLSEIWFEERRWDMENEEFLNLKFLQLRCSRIQEWNTCTDPFPCLQQLVLDCCFYL 183
           LE+LKL  +      W +++E+F  LKFL+L      EW+   D FP L+ LVL  C YL
Sbjct: 46  LEVLKLLGVTICSNTWKVKDEQFSKLKFLKLENLSFSEWDVSDDAFPYLEHLVLIRCPYL 105

Query: 184 KEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERL-DLGDDGFQVLV 327
           + IPS  G +SSL  IEV++C +    SA  I   ++ D+G   F+V++
Sbjct: 106 EVIPSCFGYMSSLKSIEVKSCKESLADSAMVIKEMQVEDMGFSDFEVII 154


>ref|XP_006363255.1| PREDICTED: putative late blight resistance protein homolog
            R1B-23-like [Solanum tuberosum]
          Length = 1261

 Score = 77.8 bits (190), Expect = 1e-12
 Identities = 38/100 (38%), Positives = 63/100 (63%), Gaps = 1/100 (1%)
 Frame = +1

Query: 1    NLEILKLSEIWFEERRWDMENEEFLNLKFLQL-RCSRIQEWNTCTDPFPCLQQLVLDCCF 177
            NL++LKL  ++F +  W++ +++F  LK L++  C   ++WN   D FP L+ LVL  C 
Sbjct: 1149 NLQVLKLVAVFFSKAEWEVTSDKFHQLKVLKVVDCPCFKKWNVSDDAFPRLEHLVLRRCR 1208

Query: 178  YLKEIPSSLGEISSLSMIEVRNCSKGAERSAWEILNERLD 297
            YL+ IPS  G+I+SL  IEV++C +   +SA  I   +++
Sbjct: 1209 YLEAIPSRFGDITSLISIEVKSCKESLVKSAMVIRESQVE 1248