BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00035173
         (447 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_175689.1| oxidoreductase, 2OG-Fe(II) oxygenase family pro...   195   4e-48
ref|XP_002284974.1| PREDICTED: gibberellin 20 oxidase 1 [Vitis v...   194   6e-48
emb|CAN80139.1| hypothetical protein VITISV_028513 [Vitis vinifera]   194   6e-48
ref|XP_002298127.1| 2-oxoglutarate-dependent dioxygenase [Populu...   186   2e-45
ref|XP_002280670.1| PREDICTED: gibberellin 3-beta-dioxygenase 3 ...   178   5e-43

>ref|NP_175689.1| oxidoreductase, 2OG-Fe(II) oxygenase family protein [Arabidopsis
           thaliana] gi|12324655|gb|AAG52291.1|AC019018_28 putative
           oxidoreductase; 33116-34434 [Arabidopsis thaliana]
           gi|67633452|gb|AAY78650.1| oxidoreductase [Arabidopsis
           thaliana] gi|332194732|gb|AEE32853.1| oxidoreductase,
           2OG-Fe(II) oxygenase family protein [Arabidopsis
           thaliana]
          Length = 314

 Score =  195 bits (495), Expect = 4e-48
 Identities = 92/149 (61%), Positives = 118/149 (79%), Gaps = 1/149 (0%)
 Frame = -2

Query: 446 LDKAIFRVADELFDLPTDTKILNKSDKPYQGYVGQIPYLPLHEGLGIDATTDEGVTN-FT 270
           LD +IF  A+ELFDLPT+TK  N ++KPY GYVGQ+P +PLHEGLG+D  T++ +   FT
Sbjct: 56  LDDSIFAAAEELFDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFT 115

Query: 269 NLMWPKGNDLFCESSLAFSKAVAELDRMVIRMLFESYGVEKYAESHVESSTYLLRYLKYR 90
           +LMWP+GND FC +   FS AVAELDR+V+RM+FE+YGVEK+ ESHV S TYLL++LKY 
Sbjct: 116 HLMWPQGNDRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYL 175

Query: 89  APEMNETTMAFPSHTDKSFLTILHQNQVS 3
           AP  + +  AFP HTDK+FL+ILHQN V+
Sbjct: 176 APPESISMPAFPQHTDKTFLSILHQNDVN 204


>ref|XP_002284974.1| PREDICTED: gibberellin 20 oxidase 1 [Vitis vinifera]
           gi|297743336|emb|CBI36203.3| unnamed protein product
           [Vitis vinifera]
          Length = 311

 Score =  194 bits (493), Expect = 6e-48
 Identities = 90/148 (60%), Positives = 117/148 (79%), Gaps = 1/148 (0%)
 Frame = -2

Query: 446 LDKAIFRVADELFDLPTDTKILNKSDKPYQGYVGQIPYLPLHEGLGI-DATTDEGVTNFT 270
           LD   F   +ELFDLPT+TK+ N SDKPY GY+GQ P +PLHE +GI DATT +GV +FT
Sbjct: 53  LDNTTFCQLEELFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFT 112

Query: 269 NLMWPKGNDLFCESSLAFSKAVAELDRMVIRMLFESYGVEKYAESHVESSTYLLRYLKYR 90
           N+MWP GND FCE+ L++SK V+EL++MV +M+FESY  EKY +SH++S+TYLLR +KYR
Sbjct: 113 NIMWPAGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYR 172

Query: 89  APEMNETTMAFPSHTDKSFLTILHQNQV 6
            P+ NET +    HTDKSF+T+LHQN+V
Sbjct: 173 GPQKNETCIGTSCHTDKSFITVLHQNEV 200


>emb|CAN80139.1| hypothetical protein VITISV_028513 [Vitis vinifera]
          Length = 311

 Score =  194 bits (493), Expect = 6e-48
 Identities = 90/148 (60%), Positives = 117/148 (79%), Gaps = 1/148 (0%)
 Frame = -2

Query: 446 LDKAIFRVADELFDLPTDTKILNKSDKPYQGYVGQIPYLPLHEGLGI-DATTDEGVTNFT 270
           LD   F   +ELFDLPT+TK+ N SDKPY GY+GQ P +PLHE +GI DATT +GV +FT
Sbjct: 53  LDNTTFCQLEELFDLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFT 112

Query: 269 NLMWPKGNDLFCESSLAFSKAVAELDRMVIRMLFESYGVEKYAESHVESSTYLLRYLKYR 90
           N+MWP GND FCE+ L++SK V+EL++MV +M+FESY  EKY +SH++S+TYLLR +KYR
Sbjct: 113 NIMWPAGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYR 172

Query: 89  APEMNETTMAFPSHTDKSFLTILHQNQV 6
            P+ NET +    HTDKSF+T+LHQN+V
Sbjct: 173 GPQKNETCIGTSCHTDKSFITVLHQNEV 200


>ref|XP_002298127.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
           gi|222845385|gb|EEE82932.1| 2-oxoglutarate-dependent
           dioxygenase [Populus trichocarpa]
          Length = 313

 Score =  186 bits (471), Expect = 2e-45
 Identities = 90/145 (62%), Positives = 112/145 (77%), Gaps = 1/145 (0%)
 Frame = -2

Query: 434 IFRVADELFDLPTDTKILNKSDKPYQGYVGQIPYLPLHEGLGID-ATTDEGVTNFTNLMW 258
           +F V  ELFDLPT+TK+ NK +KP  GYVGQI  LPLHE +GID AT+ +   NFT+LMW
Sbjct: 59  VFGVLKELFDLPTETKMQNKYEKPLNGYVGQIAKLPLHESMGIDNATSLDATQNFTDLMW 118

Query: 257 PKGNDLFCESSLAFSKAVAELDRMVIRMLFESYGVEKYAESHVESSTYLLRYLKYRAPEM 78
           P GND FCE  L ++   AELDRMV RM+FESYGVEKY +S+VES+TYLLR LK R P++
Sbjct: 119 PNGNDRFCECVLKYANLAAELDRMVTRMVFESYGVEKYHDSYVESTTYLLRLLKNRPPKV 178

Query: 77  NETTMAFPSHTDKSFLTILHQNQVS 3
           +E  + F +HTDKSF TILHQN+++
Sbjct: 179 DEHNLGFVTHTDKSFTTILHQNEIN 203


>ref|XP_002280670.1| PREDICTED: gibberellin 3-beta-dioxygenase 3 [Vitis vinifera]
           gi|297743341|emb|CBI36208.3| unnamed protein product
           [Vitis vinifera]
          Length = 317

 Score =  178 bits (451), Expect = 5e-43
 Identities = 85/148 (57%), Positives = 109/148 (73%), Gaps = 1/148 (0%)
 Frame = -2

Query: 446 LDKAIFRVADELFDLPTDTKILNKSDKPYQGYVGQIPYLPLHEGLGID-ATTDEGVTNFT 270
           L   +F   +ELF+LPT+TK+ N  +KPY GYVGQ   LPLHE +GID ATT EG+ +F 
Sbjct: 54  LQNKLFSALEELFNLPTETKMQNAHNKPYHGYVGQHSRLPLHESIGIDNATTLEGMQSFA 113

Query: 269 NLMWPKGNDLFCESSLAFSKAVAELDRMVIRMLFESYGVEKYAESHVESSTYLLRYLKYR 90
           NLMWP GND FCE +  FSK   ELD +V +M+FESYGV KY +SH+E  +YLLR LK++
Sbjct: 114 NLMWPAGNDHFCEITREFSKIALELDHLVTKMIFESYGVGKYYDSHLEPMSYLLRVLKHK 173

Query: 89  APEMNETTMAFPSHTDKSFLTILHQNQV 6
           AP++ E  + F  HTDKSF+T+L+QNQV
Sbjct: 174 APKVKEPNLGFARHTDKSFITVLYQNQV 201