BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00034475
         (602 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281827.2| PREDICTED: probable polygalacturonase At1g80...   185   6e-45
emb|CBI36284.3| unnamed protein product [Vitis vinifera]              185   6e-45
ref|NP_001234256.1| polygalacturonase [Solanum lycopersicum] gi|...   184   1e-44
gb|ABM67700.1| polygalacturonase [Citrus sinensis]                    184   1e-44
gb|AAD55489.1|AC009322_29 Similar to polygalacturonases [Arabido...   183   2e-44

>ref|XP_002281827.2| PREDICTED: probable polygalacturonase At1g80170-like [Vitis
           vinifera]
          Length = 452

 Score =  185 bits (469), Expect = 6e-45
 Identities = 85/170 (50%), Positives = 115/170 (67%)
 Frame = +2

Query: 92  MIYTIIVYAVYIFSNAECILQNQQLPQPRITNKPKPRSKNTTMLFVSDFGAKGDGFTDDT 271
           +I T+ ++ V    N E      QLP    +   +PR + T +L+V+ FGAKGDG  DDT
Sbjct: 23  LICTVFIFLVLFLINVEGFEPLIQLPH---SPAARPRLE-TKILYVNQFGAKGDGVQDDT 78

Query: 272 KAFEKVWKVACSSSSPRTKIVVPEKYSLLIRPIHFAGPCASKVTIKISGRILAPENPDVW 451
            AF   WK+AC+S + +  IV+P     L+RPI F GPC +KVT+ ISG I+AP++PDVW
Sbjct: 79  DAFRNAWKIACASIT-KASIVIPHDKKFLVRPISFGGPCRAKVTVTISGTIIAPKDPDVW 137

Query: 452 HGLNKQKWLYFIGMKNLIIEGHGVVNGMGREWWSRSCKVNTTYPCQHAPT 601
           HGLN QKWLYF G+ +L + G G++NG G +WWS+SCK+N T PC+HAPT
Sbjct: 138 HGLNPQKWLYFHGVNHLTVRGSGIINGRGHKWWSQSCKINATNPCEHAPT 187


>emb|CBI36284.3| unnamed protein product [Vitis vinifera]
          Length = 441

 Score =  185 bits (469), Expect = 6e-45
 Identities = 85/170 (50%), Positives = 115/170 (67%)
 Frame = +2

Query: 92  MIYTIIVYAVYIFSNAECILQNQQLPQPRITNKPKPRSKNTTMLFVSDFGAKGDGFTDDT 271
           +I T+ ++ V    N E      QLP    +   +PR + T +L+V+ FGAKGDG  DDT
Sbjct: 12  LICTVFIFLVLFLINVEGFEPLIQLPH---SPAARPRLE-TKILYVNQFGAKGDGVQDDT 67

Query: 272 KAFEKVWKVACSSSSPRTKIVVPEKYSLLIRPIHFAGPCASKVTIKISGRILAPENPDVW 451
            AF   WK+AC+S + +  IV+P     L+RPI F GPC +KVT+ ISG I+AP++PDVW
Sbjct: 68  DAFRNAWKIACASIT-KASIVIPHDKKFLVRPISFGGPCRAKVTVTISGTIIAPKDPDVW 126

Query: 452 HGLNKQKWLYFIGMKNLIIEGHGVVNGMGREWWSRSCKVNTTYPCQHAPT 601
           HGLN QKWLYF G+ +L + G G++NG G +WWS+SCK+N T PC+HAPT
Sbjct: 127 HGLNPQKWLYFHGVNHLTVRGSGIINGRGHKWWSQSCKINATNPCEHAPT 176


>ref|NP_001234256.1| polygalacturonase [Solanum lycopersicum]
           gi|7381227|gb|AAF61444.1|AF138858_1 polygalacturonase
           [Solanum lycopersicum]
          Length = 452

 Score =  184 bits (467), Expect = 1e-44
 Identities = 88/182 (48%), Positives = 126/182 (69%)
 Frame = +2

Query: 56  LQSFHVSADPTYMIYTIIVYAVYIFSNAECILQNQQLPQPRITNKPKPRSKNTTMLFVSD 235
           + SF+ S+    +   + +Y++Y    +  +   + L Q  +      R+K+ ++  V+D
Sbjct: 9   ISSFNFSSYSKSVALFLSLYSLYFLLISANVSGFESLLQ-LLPASASLRTKSESLFRVND 67

Query: 236 FGAKGDGFTDDTKAFEKVWKVACSSSSPRTKIVVPEKYSLLIRPIHFAGPCASKVTIKIS 415
           FGA GDG TDDTK+F+ VW +ACSS S   KIV+P  YS L+R I+FAGPC SKV+I+I+
Sbjct: 68  FGATGDGITDDTKSFKDVWDMACSSPS-HAKIVIPAGYSFLVRQINFAGPCRSKVSIRIA 126

Query: 416 GRILAPENPDVWHGLNKQKWLYFIGMKNLIIEGHGVVNGMGREWWSRSCKVNTTYPCQHA 595
           G ILAP++PDVW  LN +KW+YF  +K+L +EG G+++GMG+EWW+RSCKVN T PC HA
Sbjct: 127 GTILAPKDPDVWDDLNPRKWIYFFKVKHLTVEGGGIIDGMGQEWWARSCKVNRTNPCHHA 186

Query: 596 PT 601
           PT
Sbjct: 187 PT 188


>gb|ABM67700.1| polygalacturonase [Citrus sinensis]
          Length = 445

 Score =  184 bits (467), Expect = 1e-44
 Identities = 81/134 (60%), Positives = 105/134 (78%)
 Frame = +2

Query: 200 RSKNTTMLFVSDFGAKGDGFTDDTKAFEKVWKVACSSSSPRTKIVVPEKYSLLIRPIHFA 379
           RSK+  ++FV DFGAKGDGF DDT+AF   WK ACS  + RTKIV    Y+ LI PI  +
Sbjct: 48  RSKSKRVIFVGDFGAKGDGFNDDTEAFANAWKKACSFPA-RTKIVFSAGYTFLIHPIDIS 106

Query: 380 GPCASKVTIKISGRILAPENPDVWHGLNKQKWLYFIGMKNLIIEGHGVVNGMGREWWSRS 559
           GPC S++T+KISG I+AP++PDVW GLN+++WLYF  + +L ++G G +NGMG+EWWSRS
Sbjct: 107 GPCKSRLTLKISGTIVAPKDPDVWKGLNRRRWLYFNRVNHLTVQGGGTINGMGQEWWSRS 166

Query: 560 CKVNTTYPCQHAPT 601
           CK+NTT PC+HAPT
Sbjct: 167 CKINTTNPCRHAPT 180


>gb|AAD55489.1|AC009322_29 Similar to polygalacturonases [Arabidopsis thaliana]
          Length = 459

 Score =  183 bits (464), Expect = 2e-44
 Identities = 86/172 (50%), Positives = 121/172 (70%)
 Frame = +2

Query: 86  TYMIYTIIVYAVYIFSNAECILQNQQLPQPRITNKPKPRSKNTTMLFVSDFGAKGDGFTD 265
           T ++  ++  ++ + +NA       QLP+ R +   +PRS+   +L V +FGAKG+G TD
Sbjct: 11  TLLLLLVVASSLALTANANSFESLLQLPR-RQSRSTRPRSER--LLHVGNFGAKGNGVTD 67

Query: 266 DTKAFEKVWKVACSSSSPRTKIVVPEKYSLLIRPIHFAGPCASKVTIKISGRILAPENPD 445
           DTKAF   WK ACSS   +T+I+VPE Y+ L+RPI  +GPC +++T++ISG I+AP +PD
Sbjct: 68  DTKAFADAWKTACSSKV-KTRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPD 126

Query: 446 VWHGLNKQKWLYFIGMKNLIIEGHGVVNGMGREWWSRSCKVNTTYPCQHAPT 601
           VW GLN++KWLYF G+  L +EG G VNGMG+EWW RSCK N + PC+ APT
Sbjct: 127 VWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPT 178