BLASTX nr result
ID: Bupleurum21_contig00034440
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00034440
(641 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM82604.1|AF525305_2 putative AP endonuclease/reverse transc... 99 7e-21
emb|CAA18234.1| putative protein [Arabidopsis thaliana] gi|72694... 94 2e-20
ref|XP_002331338.1| predicted protein [Populus trichocarpa] gi|2... 98 1e-18
gb|AAC28221.1| similar to reverse transcriptases (PFam: rvt.hmm,... 89 2e-18
dbj|BAB01845.1| non-LTR retroelement reverse transcriptase-like ... 92 2e-18
>gb|AAM82604.1|AF525305_2 putative AP endonuclease/reverse transcriptase [Brassica napus]
Length = 1214
Score = 98.6 bits (244), Expect(2) = 7e-21
Identities = 53/177 (29%), Positives = 94/177 (53%), Gaps = 2/177 (1%)
Frame = -2
Query: 628 LTDLRCIGCDFTWWDSNDTSPLYRKLDRVLVNDTWLSEYPMSIANFHPRALSDHSPAVVS 449
++DL G +TWW++ + +P+ +K+DR+LVND+WL P+S +F SDH P+ V+
Sbjct: 176 ISDLPFRGNHYTWWNNQENNPIAKKIDRILVNDSWLIASPLSYGSFCAMEFSDHCPSCVN 235
Query: 448 LGDTGQRIKKPFMLFHHMLEDPDFMEEVQKAWD-CTISGSPWFILTTKLRMVKAAMIQLN 272
+ + KPF L + ++ P+F+E+++ WD GS F L+ K + +K + N
Sbjct: 236 ISNQSGGRNKPFKLSNFLMHHPEFIEKIRVTWDRLAYQGSAMFTLSKKSKFLKGTIRTFN 295
Query: 271 HKR-GNVQTAVDIARQNLLQFQASMPSCPTYAQHEEESRLSSIYSKALSTEEQFLKQ 104
+ ++ V A QNL Q ++ + P+ E +++ EE+FL Q
Sbjct: 296 REHYSGLEKRVVQAAQNLKTCQNNLLAAPSSYLAGLEKEAHRSWAELALAEERFLCQ 352
Score = 27.7 bits (60), Expect(2) = 7e-21
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -1
Query: 104 KSRIHWLQHGDGNNRFFFNACQGRWNRNKI 15
KSR+ WL+ GD N FF R N+I
Sbjct: 353 KSRVLWLKCGDSNTTFFHRMMTARRAINEI 382
>emb|CAA18234.1| putative protein [Arabidopsis thaliana] gi|7269488|emb|CAB79491.1|
putative protein [Arabidopsis thaliana]
Length = 1141
Score = 94.0 bits (232), Expect(2) = 2e-20
Identities = 54/177 (30%), Positives = 89/177 (50%), Gaps = 2/177 (1%)
Frame = -2
Query: 628 LTDLRCIGCDFTWWDSNDTSPLYRKLDRVLVNDTWLSEYPMSIANFHPRALSDHSPAVVS 449
L+DL G FTWW+ + T P+ +K+DR+LVN++W + +P S F P SDH+ V
Sbjct: 170 LSDLVYKGSSFTWWNKSKTRPVAKKIDRILVNESWSNLFPSSFGLFGPPDFSDHASCGVV 229
Query: 448 LGDTGQRIKKPFMLFHHMLEDPDFMEEVQKAWDCT-ISGSPWFILTTKLRMVKAAMIQLN 272
L + K+PF F+ +L++P+F+ V W T + GS F ++ KL+ +K + +
Sbjct: 230 LELDPIKAKRPFKFFNFLLKNPEFLNLVWDVWYSTNVVGSSMFRVSKKLKALKKPIKDFS 289
Query: 271 H-KRGNVQTAVDIARQNLLQFQASMPSCPTYAQHEEESRLSSIYSKALSTEEQFLKQ 104
N++ + A + LL FQ P+ E + + EE F +Q
Sbjct: 290 RLNYSNLEKRTEEAHETLLSFQNLTLDNPSLENAAHELEAQRKWQILATAEESFFRQ 346
Score = 30.8 bits (68), Expect(2) = 2e-20
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 104 KSRIHWLQHGDGNNRFFFNACQGRWNRNKIMAI 6
+SR+ W GDGN R+F R + N I +
Sbjct: 347 RSRVTWFAEGDGNTRYFHRMADSRKSVNTITTL 379
>ref|XP_002331338.1| predicted protein [Populus trichocarpa] gi|222873371|gb|EEF10502.1|
predicted protein [Populus trichocarpa]
Length = 819
Score = 98.2 bits (243), Expect = 1e-18
Identities = 62/187 (33%), Positives = 91/187 (48%), Gaps = 4/187 (2%)
Frame = -2
Query: 622 DLRCIGCDFTWWDSNDTSPLYRKLDRVLVNDTWLSEYPMSIANFHPRALSDHSPAVVSLG 443
DLR G +TW + + + RKLDRVLVN+ W ++P+S A F P +SDHSP VV +
Sbjct: 553 DLRYSGMHYTWSNQCPENLIMRKLDRVLVNEKWNLKFPLSEARFLPSGMSDHSPMVVKVI 612
Query: 442 DTGQRIKKPFMLFHHMLEDPDFMEEVQKAWDCTISGSPWFILTTKLRMVKAAMIQLNHKR 263
Q KKPF F ++ +FM V+K WD G P + L KLR +K + N
Sbjct: 613 GNDQNKKKPFRFFDMWMDHDEFMPLVKKVWDQNSRGCPMYQLCCKLRKLKQELKLFNMAH 672
Query: 262 -GNVQTAVDIARQNLLQFQASMPSC---PTYAQHEEESRLSSIYSKALSTEEQFLKQNLA 95
N+ V A+ + + Q ++ + P E + + Y+ + EE F KQ
Sbjct: 673 FSNISDRVRDAKNKMDKAQQALHTAHENPILCMRERD--VVHKYASTVRAEESFFKQKAR 730
Query: 94 SIGCSMG 74
S+G
Sbjct: 731 IQWLSLG 737
>gb|AAC28221.1| similar to reverse transcriptases (PFam: rvt.hmm, score: 60.13)
[Arabidopsis thaliana]
Length = 1164
Score = 89.0 bits (219), Expect(2) = 2e-18
Identities = 52/161 (32%), Positives = 84/161 (52%), Gaps = 4/161 (2%)
Frame = -2
Query: 628 LTDLRCIGCDFTWWDSNDTSPLYRKLDRVLVNDTWLSEYPMSIANFHPRALSDHSPAVVS 449
LTDL G FTWW+ +P+ +KLDR+LVND W + +P S+ F SDHS +S
Sbjct: 75 LTDLSFRGNTFTWWNKRSRAPVAKKLDRILVNDKWTTTFPSSLGLFGEPDFSDHSSCELS 134
Query: 448 LGDTGQRIKKPFMLFHHMLEDPDFMEEVQKAWDCT-ISGSPWFILTTKLRMVKAAMIQLN 272
L R KKPF + +L+D +F+ + W T ++GS + ++ KL+ +K + +
Sbjct: 135 LMSASPRSKKPFRFNNFLLKDENFLSLICLKWFSTSVTGSAMYRVSVKLKALKKVIRDFS 194
Query: 271 HKR-GNVQTAVDIARQNLLQFQASM--PSCPTYAQHEEESR 158
+++ A LL Q+ + CP+ A E E++
Sbjct: 195 RDNYSDIEKRTKEAHDALLLAQSVLLASPCPSNAAIEAETQ 235
Score = 29.3 bits (64), Expect(2) = 2e-18
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -1
Query: 104 KSRIHWLQHGDGNNRFFFNACQGRWNRNKI 15
+SR++WL+ GD N+ +F R + N I
Sbjct: 252 RSRVNWLREGDMNSSYFHKMASARQSLNHI 281
>dbj|BAB01845.1| non-LTR retroelement reverse transcriptase-like protein
[Arabidopsis thaliana]
Length = 893
Score = 92.4 bits (228), Expect(2) = 2e-18
Identities = 53/177 (29%), Positives = 89/177 (50%), Gaps = 2/177 (1%)
Frame = -2
Query: 628 LTDLRCIGCDFTWWDSNDTSPLYRKLDRVLVNDTWLSEYPMSIANFHPRALSDHSPAVVS 449
L DL G +TWW+ + PL +K+DR+LVND W + +P + ANF SDHS V
Sbjct: 175 LYDLVYKGSSYTWWNKCSSRPLAKKIDRILVNDHWNTLFPSAYANFGEPDFSDHSSCEVV 234
Query: 448 LGDTGQRIKKPFMLFHHMLEDPDFMEEVQKAW-DCTISGSPWFILTTKLRMVKAAMIQLN 272
L + K+PF F++ L +PDF++ +++ W C +SGS + ++ KL+ +K + +
Sbjct: 235 LDPAVLKAKRPFRFFNYFLHNPDFLQLIRENWYSCNVSGSAMYRVSKKLKHLKLPICCFS 294
Query: 271 HKR-GNVQTAVDIARQNLLQFQASMPSCPTYAQHEEESRLSSIYSKALSTEEQFLKQ 104
+ +++ V A +L Q + P+ E + + EE F Q
Sbjct: 295 RENYSDIEKRVSEAHAIVLHRQRITLTNPSVVHATLELEATRKWQILAKAEESFFCQ 351
Score = 25.8 bits (55), Expect(2) = 2e-18
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = -1
Query: 104 KSRIHWLQHGDGNNRFFFNACQGRWNRNKI 15
KS I WL GD N +F R + N I
Sbjct: 352 KSSISWLYEGDNNTAYFHKMADMRKSINTI 381