BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00032769
         (1300 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265768.1| PREDICTED: rhomboid protein 1, mitochondrial...   283   9e-74
emb|CBI15323.3| unnamed protein product [Vitis vinifera]              282   2e-73
ref|XP_002309495.1| predicted protein [Populus trichocarpa] gi|2...   254   3e-65
ref|XP_004137444.1| PREDICTED: uncharacterized protein C13E7.11-...   246   1e-62
ref|NP_564058.1| RHOMBOID-like protein 12 [Arabidopsis thaliana]...   238   3e-60

>ref|XP_002265768.1| PREDICTED: rhomboid protein 1, mitochondrial [Vitis vinifera]
          Length = 333

 Score =  283 bits (723), Expect = 9e-74
 Identities = 167/325 (51%), Positives = 205/325 (63%), Gaps = 20/325 (6%)
 Frame = +1

Query: 94   MQRFLSLKLLIKNHATSNGTSYISNRCFSLTKTNSICPQANLVSQFSSTALS-------N 252
            MQR +SLK+           S IS R F  TK +S+       + FS +  S        
Sbjct: 1    MQRLISLKV----------ASDISRRVF--TKPSSLLHSHPNKTFFSLSQPSPTHHLHTT 48

Query: 253  SRVKPHNF------------YDLLSNPLLVNQFLQKTGALLKATSKGVVDCKWVFPRSYF 396
            S + P +F            Y  +SNP L+ QFL    ALLK +SK +   +  F R+ F
Sbjct: 49   SLIPPPHFPWPSHHGLSRSVYGFISNPTLLKQFLPT--ALLKGSSKTLAASRVGFLRAQF 106

Query: 397  LQKKSSFGLVSSRSFNRGRRWIQRLDSDHVVLGLIAANVSVFLLWRLADHRFMTNNFMIS 576
             ++   F         R R W+  L ++ VVLGLI ANV+VF+LWR++DHRFM NNF IS
Sbjct: 107  PRRSFGFSPSFDSYGRRWRSWLSGLSTNDVVLGLIIANVAVFMLWRISDHRFMLNNFTIS 166

Query: 577  VDNFRSGRIHTMITSAFSHIDARHIISNMIGLYFFGNSIGRSFGPEFXXXXXXXXXXXXS 756
            +DNF+SGRIHT+ITSAFSHID  H+ISNMIGLYFFG +IGR FGPEF            S
Sbjct: 167  LDNFKSGRIHTLITSAFSHIDIGHLISNMIGLYFFGMNIGRVFGPEFLLKLYLAGAVVGS 226

Query: 757  VFYLVYHAFLIP-SKSNQMWGRDPSSVPGLGASGAVNAIMLLDIFLNPQKTIFFEMIIPV 933
            VFYLV+HAF+ P SK  QMW  +PS++PGLGASGAVNAIMLLDIFL P+ T++ E  IPV
Sbjct: 227  VFYLVHHAFMAPSSKGQQMWSINPSAIPGLGASGAVNAIMLLDIFLFPRSTLYLEFFIPV 286

Query: 934  PAILVGIFLIGKDMLNIIEGDTQVS 1008
            PAIL+GIFLIGKDML IIEGD Q+S
Sbjct: 287  PAILLGIFLIGKDMLRIIEGDDQIS 311


>emb|CBI15323.3| unnamed protein product [Vitis vinifera]
          Length = 362

 Score =  282 bits (721), Expect = 2e-73
 Identities = 147/245 (60%), Positives = 177/245 (72%), Gaps = 1/245 (0%)
 Frame = +1

Query: 277  YDLLSNPLLVNQFLQKTGALLKATSKGVVDCKWVFPRSYFLQKKSSFGLVSSRSFNRGRR 456
            Y  +SNP L+ QFL    ALLK +SK +   +  F R+ F ++   F         R R 
Sbjct: 98   YGFISNPTLLKQFLPT--ALLKGSSKTLAASRVGFLRAQFPRRSFGFSPSFDSYGRRWRS 155

Query: 457  WIQRLDSDHVVLGLIAANVSVFLLWRLADHRFMTNNFMISVDNFRSGRIHTMITSAFSHI 636
            W+  L ++ VVLGLI ANV+VF+LWR++DHRFM NNF IS+DNF+SGRIHT+ITSAFSHI
Sbjct: 156  WLSGLSTNDVVLGLIIANVAVFMLWRISDHRFMLNNFTISLDNFKSGRIHTLITSAFSHI 215

Query: 637  DARHIISNMIGLYFFGNSIGRSFGPEFXXXXXXXXXXXXSVFYLVYHAFLIP-SKSNQMW 813
            D  H+ISNMIGLYFFG +IGR FGPEF            SVFYLV+HAF+ P SK  QMW
Sbjct: 216  DIGHLISNMIGLYFFGMNIGRVFGPEFLLKLYLAGAVVGSVFYLVHHAFMAPSSKGQQMW 275

Query: 814  GRDPSSVPGLGASGAVNAIMLLDIFLNPQKTIFFEMIIPVPAILVGIFLIGKDMLNIIEG 993
              +PS++PGLGASGAVNAIMLLDIFL P+ T++ E  IPVPAIL+GIFLIGKDML IIEG
Sbjct: 276  SINPSAIPGLGASGAVNAIMLLDIFLFPRSTLYLEFFIPVPAILLGIFLIGKDMLRIIEG 335

Query: 994  DTQVS 1008
            D Q+S
Sbjct: 336  DDQIS 340


>ref|XP_002309495.1| predicted protein [Populus trichocarpa] gi|222855471|gb|EEE93018.1|
            predicted protein [Populus trichocarpa]
          Length = 242

 Score =  254 bits (649), Expect = 3e-65
 Identities = 130/241 (53%), Positives = 162/241 (67%), Gaps = 1/241 (0%)
 Frame = +1

Query: 358  VVDCKWVFPRSYFLQKKSSFGLVSSRSFNRGRRWIQRLDSDHVVLGLIAANVSVFLLWRL 537
            + DCK  F  + F +  S   L    +    R W  RL +D+VVLGLI AN +VF+LWR+
Sbjct: 2    LTDCKVGFFIARFARGYSWSNLSLGSNRRGWRSWFNRLSADNVVLGLIIANAAVFMLWRI 61

Query: 538  ADHRFMTNNFMISVDNFRSGRIHTMITSAFSHIDARHIISNMIGLYFFGNSIGRSFGPEF 717
             D +FM  NFMIS+DNFRSGRIHT++TSAFSHID  HI  NMIGLYFFG +I R+FGPEF
Sbjct: 62   EDQKFMMENFMISLDNFRSGRIHTLVTSAFSHIDIGHIAFNMIGLYFFGTNIARTFGPEF 121

Query: 718  XXXXXXXXXXXXSVFYLVYHAFL-IPSKSNQMWGRDPSSVPGLGASGAVNAIMLLDIFLN 894
                        SVFYL++H ++ + SK   MW RD S  PGLGASGAVNAIMLLDIFLN
Sbjct: 122  LLKLYLAGAIGGSVFYLLHHGYMDLSSKGQGMWARDSSRTPGLGASGAVNAIMLLDIFLN 181

Query: 895  PQKTIFFEMIIPVPAILVGIFLIGKDMLNIIEGDTQVSXXXXXXXXXXXXXXXXRYRRGR 1074
            P+ T++F+ IIPVPAIL+GIFLIGKD+L ++EG++ +S                R +RGR
Sbjct: 182  PRATLYFDFIIPVPAILLGIFLIGKDLLRVMEGNSNISGSAHLGGAAVAAIAWARIKRGR 241

Query: 1075 F 1077
            F
Sbjct: 242  F 242


>ref|XP_004137444.1| PREDICTED: uncharacterized protein C13E7.11-like [Cucumis sativus]
          Length = 336

 Score =  246 bits (627), Expect = 1e-62
 Identities = 136/286 (47%), Positives = 172/286 (60%), Gaps = 2/286 (0%)
 Frame = +1

Query: 226  QFSSTALSNSRVKPHNFYDLLSNPLLVNQFL-QKTGALLKATSKGVVDCKWVFPRSYFLQ 402
            Q  S + S+ R      +   S P++  +F+   +    K + K VV+ + +F  + F +
Sbjct: 51   QDHSRSWSSHRSFAQKIHGFFSVPVVGKRFIVNPSNTYFKGSMKSVVESRSLFTGTPFSK 110

Query: 403  KKSSFGLVSSRSFNRGRRWIQRLDSDHVVLGLIAANVSVFLLWRLADHRFMTNNFMISVD 582
             K          +   R W QRL  + VVLGLI ANV+VFLLWR+AD  FM+ NF IS++
Sbjct: 111  LKFQLKPGFVHQWGGRRSWFQRLTPNEVVLGLIIANVAVFLLWRIADPIFMSKNFTISLE 170

Query: 583  NFRSGRIHTMITSAFSHIDARHIISNMIGLYFFGNSIGRSFGPEFXXXXXXXXXXXXSVF 762
            N RSGRIHT+ITSAFSHID  HI+SNM+GLYFFG +IG  FG EF            S F
Sbjct: 171  NVRSGRIHTLITSAFSHIDTGHIVSNMVGLYFFGMNIGNVFGSEFLLKLYLAGAIGGSAF 230

Query: 763  YLVYHAF-LIPSKSNQMWGRDPSSVPGLGASGAVNAIMLLDIFLNPQKTIFFEMIIPVPA 939
            YL +H F  + SKS   WG DP    GLGASGAVNAIMLLDIFL P+ T++ +  IPVPA
Sbjct: 231  YLAHHLFQALSSKSRSFWGEDPVRAKGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPA 290

Query: 940  ILVGIFLIGKDMLNIIEGDTQVSXXXXXXXXXXXXXXXXRYRRGRF 1077
            IL+GIFLIGKDML ++E D+Q+S                R RRGRF
Sbjct: 291  ILLGIFLIGKDMLRMLENDSQISGSAHLGGAAVAALAWARVRRGRF 336


>ref|NP_564058.1| RHOMBOID-like protein 12 [Arabidopsis thaliana]
            gi|9795596|gb|AAF98414.1|AC026238_6 Hypothetical protein
            [Arabidopsis thaliana]
            gi|13877607|gb|AAK43881.1|AF370504_1 Unknown protein
            [Arabidopsis thaliana] gi|20148713|gb|AAM10247.1| unknown
            protein [Arabidopsis thaliana] gi|21592397|gb|AAM64348.1|
            unknown [Arabidopsis thaliana]
            gi|332191612|gb|AEE29733.1| RHOMBOID-like protein 12
            [Arabidopsis thaliana]
          Length = 336

 Score =  238 bits (606), Expect = 3e-60
 Identities = 126/249 (50%), Positives = 162/249 (65%), Gaps = 3/249 (1%)
 Frame = +1

Query: 340  KATSKG-VVDCKWVFPRSYFLQKKSSFGLVSSRSFNRGRRWIQRLDSDHVVLGLIAANVS 516
            KA   G +V+ +  F  S F +K   F   S       + W+Q L    VVLGL+ AN  
Sbjct: 86   KAVKLGNLVESRVGFIGSQFPKKGFEFQRFSGFQRRGWKHWLQGLSDRDVVLGLVIANAG 145

Query: 517  VFLLWRLADHRFMTNNFMISVDNFRSGRIHTMITSAFSHIDARHIISNMIGLYFFGNSIG 696
            VF++WR+ + +FM NNFMIS+DNF+SGR+HT+ITSAFSHID  HI+SNMIGLYFFG SI 
Sbjct: 146  VFVMWRVFNQQFMMNNFMISLDNFKSGRLHTLITSAFSHIDIGHIVSNMIGLYFFGTSIA 205

Query: 697  RSFGPEFXXXXXXXXXXXXSVFYLVYHAFLIPS--KSNQMWGRDPSSVPGLGASGAVNAI 870
            R+FGP+F            SVFYL++HA++  +  K    + RDPS  PGLGASGAVNAI
Sbjct: 206  RNFGPQFLLKLYLAGALGGSVFYLIHHAYMAATSPKGQGAFVRDPSRTPGLGASGAVNAI 265

Query: 871  MLLDIFLNPQKTIFFEMIIPVPAILVGIFLIGKDMLNIIEGDTQVSXXXXXXXXXXXXXX 1050
            MLLDIFL+P+ T++ E  IPVPA+L+GIFLIGKD+L I EG++ +S              
Sbjct: 266  MLLDIFLHPRATLYLEFFIPVPAMLLGIFLIGKDILRITEGNSNISGSAHLGGAAVAAIA 325

Query: 1051 XXRYRRGRF 1077
              R R+GRF
Sbjct: 326  WARIRKGRF 334