BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00032527
         (382 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGC65498.1| FAD2-1 protein [Carthamus tinctorius]                  144   6e-33
ref|XP_004170289.1| PREDICTED: omega-6 fatty acid desaturase, en...   143   1e-32
ref|XP_004136572.1| PREDICTED: omega-6 fatty acid desaturase, en...   143   1e-32
gb|ABY71269.1| delta-12 fatty acid desaturase [Gossypium hirsutum]    143   2e-32
gb|ABF70320.1| delta-12 desaturase [Isatis tinctoria] gi|1022306...   140   8e-32

>gb|AGC65498.1| FAD2-1 protein [Carthamus tinctorius]
          Length = 380

 Score =  144 bits (364), Expect = 6e-33
 Identities = 64/127 (50%), Positives = 87/127 (68%)
 Frame = -2

Query: 381 FQRSIFRSFSYLAFDIMFAYFFHMMATTYVINKPESFSSGLIYLVNWSIYAVVQGCVFGA 202
           F RS+ RSFSY+ +D+  A+ F+ +ATTY+   P  FS    YL  W  Y +VQGCV   
Sbjct: 45  FNRSLIRSFSYIVYDLAIAFVFYYLATTYIHRLPTPFS----YLA-WLAYWIVQGCVLTG 99

Query: 201 FWFIGHEAGHHAFTEYEWLDDIVGFITHTFILWPYFSFKFSHRRHHGKTGHLSQEEVETP 22
            W + HE GHHAF++Y+W+DD VGFI H+F+L PYFS+K+SHRRHH  T  L ++EV  P
Sbjct: 100 AWVVAHECGHHAFSDYQWVDDTVGFIVHSFLLVPYFSWKYSHRRHHSNTASLERDEVFVP 159

Query: 21  MLKSEVP 1
             +S++P
Sbjct: 160 KPRSKLP 166


>ref|XP_004170289.1| PREDICTED: omega-6 fatty acid desaturase, endoplasmic
           reticulum-like, partial [Cucumis sativus]
          Length = 242

 Score =  143 bits (361), Expect = 1e-32
 Identities = 63/126 (50%), Positives = 85/126 (67%)
 Frame = -2

Query: 381 FQRSIFRSFSYLAFDIMFAYFFHMMATTYVINKPESFSSGLIYLVNWSIYAVVQGCVFGA 202
           FQRS+ RSFSY+ +D+  A  F+ +ATTY  N P      L++   W++Y   QGCV   
Sbjct: 45  FQRSVLRSFSYVVYDLTLASIFYYIATTYFQNLPS-----LLFYPAWALYWAAQGCVLTG 99

Query: 201 FWFIGHEAGHHAFTEYEWLDDIVGFITHTFILWPYFSFKFSHRRHHGKTGHLSQEEVETP 22
            W I HE GHHAF++Y+WLDD VG I H+F++ PYFS+K+SHRRHH  TG L ++EV  P
Sbjct: 100 VWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVP 159

Query: 21  MLKSEV 4
             KS++
Sbjct: 160 KQKSDM 165


>ref|XP_004136572.1| PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum
           isozyme 2-like [Cucumis sativus]
          Length = 382

 Score =  143 bits (361), Expect = 1e-32
 Identities = 63/126 (50%), Positives = 85/126 (67%)
 Frame = -2

Query: 381 FQRSIFRSFSYLAFDIMFAYFFHMMATTYVINKPESFSSGLIYLVNWSIYAVVQGCVFGA 202
           FQRS+ RSFSY+ +D+  A  F+ +ATTY  N P      L++   W++Y   QGCV   
Sbjct: 45  FQRSVLRSFSYVVYDLTLASIFYYIATTYFQNLPS-----LLFYPAWALYWAAQGCVLTG 99

Query: 201 FWFIGHEAGHHAFTEYEWLDDIVGFITHTFILWPYFSFKFSHRRHHGKTGHLSQEEVETP 22
            W I HE GHHAF++Y+WLDD VG I H+F++ PYFS+K+SHRRHH  TG L ++EV  P
Sbjct: 100 VWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVP 159

Query: 21  MLKSEV 4
             KS++
Sbjct: 160 KQKSDM 165


>gb|ABY71269.1| delta-12 fatty acid desaturase [Gossypium hirsutum]
          Length = 234

 Score =  143 bits (360), Expect = 2e-32
 Identities = 63/124 (50%), Positives = 84/124 (67%)
 Frame = -2

Query: 381 FQRSIFRSFSYLAFDIMFAYFFHMMATTYVINKPESFSSGLIYLVNWSIYAVVQGCVFGA 202
           F+RS+ RSFSYL +D++ A  F+ +AT Y  + P+         V W +Y  +QGC+F A
Sbjct: 30  FKRSVLRSFSYLIYDLVLASLFYYVATNYFPSLPQPLP-----YVAWPLYWAMQGCIFTA 84

Query: 201 FWFIGHEAGHHAFTEYEWLDDIVGFITHTFILWPYFSFKFSHRRHHGKTGHLSQEEVETP 22
           FW + HE GH AF++Y+WLDD +GFI HTF+L PYFS K+SHRRHH  TG L ++EV  P
Sbjct: 85  FWALAHECGHQAFSDYQWLDDTIGFILHTFLLTPYFSLKYSHRRHHSNTGSLERDEVFVP 144

Query: 21  MLKS 10
             KS
Sbjct: 145 KKKS 148


>gb|ABF70320.1| delta-12 desaturase [Isatis tinctoria] gi|102230686|gb|ABF70321.1|
           delta-12 desaturase [Isatis tinctoria]
          Length = 260

 Score =  140 bits (354), Expect = 8e-32
 Identities = 68/126 (53%), Positives = 86/126 (68%)
 Frame = -2

Query: 381 FQRSIFRSFSYLAFDIMFAYFFHMMATTYVINKPESFSSGLIYLVNWSIYAVVQGCVFGA 202
           F+RSI RSFSYL +DI+ A  F+ +ATTY    P+  S    YL  W +Y V QGCV   
Sbjct: 38  FKRSIPRSFSYLIWDIIIASCFYFVATTYFSLLPQPLS----YLA-WPLYWVCQGCVLTG 92

Query: 201 FWFIGHEAGHHAFTEYEWLDDIVGFITHTFILWPYFSFKFSHRRHHGKTGHLSQEEVETP 22
            W I HE GHHAF++Y+WLDD VG I H+F+L PYFS+K+SHRRHH  TG L ++EV  P
Sbjct: 93  VWVIAHECGHHAFSDYQWLDDTVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVP 152

Query: 21  MLKSEV 4
             KS++
Sbjct: 153 KQKSDI 158