BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00032339
         (1019 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264085.1| PREDICTED: hyoscyamine 6-dioxygenase [Vitis ...   278   2e-72
ref|XP_002306841.1| predicted protein [Populus trichocarpa] gi|2...   277   3e-72
ref|XP_003632389.1| PREDICTED: LOW QUALITY PROTEIN: hyoscyamine ...   274   2e-71
gb|AAM48133.1|AF509338_1 putative flavanone 3-hydroxylase [Sauss...   269   9e-70
ref|XP_002330260.1| predicted protein [Populus trichocarpa] gi|2...   268   2e-69

>ref|XP_002264085.1| PREDICTED: hyoscyamine 6-dioxygenase [Vitis vinifera]
           gi|297744130|emb|CBI37100.3| unnamed protein product
           [Vitis vinifera]
          Length = 341

 Score =  278 bits (710), Expect = 2e-72
 Identities = 139/273 (50%), Positives = 186/273 (68%), Gaps = 1/273 (0%)
 Frame = +2

Query: 2   INHGVPDDLMQETISLYEEFFNLPVEVNENLLSDDFYKSVRLYTSGFNYANEEYHLWKDT 181
           INHGV D+L+ E+ S++ EFFN+P E   +L S D  KS  LYTS  NY  EE HLW+D 
Sbjct: 71  INHGVSDNLINESRSIFSEFFNMPAEDKASLYSTDIDKSCILYTSNLNYDIEEVHLWRDN 130

Query: 182 LKHLTYPLEDVIQGWPDKPARYRDVMAKYSEEVRKMCHRILDLICEGLGLEDGFFRGELS 361
           L+H  +P E+ +Q WP+KP +YR+++  +S EVRK+  +ILDLI EGLGLE G+F GELS
Sbjct: 131 LRHPCHPSEEYVQFWPEKPTKYREIVGAFSVEVRKLSLKILDLISEGLGLEQGYFGGELS 190

Query: 362 KGLGMVINNYPPCPEPSLAMGIHPHCDPYILTI-IQQQVYGLQIKRKDQWIGVEPLANAF 538
           K   + +N+YP CP+PSL +G+  H DP ++ + +Q  V GLQ+ +  QW+GVEPL  AF
Sbjct: 191 KRQLLSVNHYPRCPDPSLTLGLPKHIDPCLIAVLLQGDVDGLQVYKDGQWLGVEPLPYAF 250

Query: 539 VIMIPYQLQVISNGKLSSCEHRGVTNSSTARISIVTFCGPTQECIIGPAKEHVSASNPPR 718
           VI I +QLQVISNGKL   EHR VTN   AR +  TF  P+ +C+I PA+  V+ASNPP 
Sbjct: 251 VINIGHQLQVISNGKLRGAEHRVVTNPREARTTPTTFINPSPDCVIQPAEALVNASNPPL 310

Query: 719 FKPYKYLDFQINYLYHLSKKNLCSETALGPYLL 817
           +K +K++DF  N+    +  N   ETAL PY L
Sbjct: 311 YKAFKFIDFFKNF----TAANGDPETALNPYKL 339


>ref|XP_002306841.1| predicted protein [Populus trichocarpa] gi|222856290|gb|EEE93837.1|
           predicted protein [Populus trichocarpa]
          Length = 345

 Score =  277 bits (708), Expect = 3e-72
 Identities = 131/256 (51%), Positives = 179/256 (69%), Gaps = 3/256 (1%)
 Frame = +2

Query: 2   INHGVPDDLMQETISLYEEFFNLPVEVNENLLSDDFY--KSVRLYTSGFNYANEEYHLWK 175
           +NHGVP+DLM++T+ +++EFF LP E      ++D    K  +LY S   YA E+ HLW+
Sbjct: 72  VNHGVPEDLMKDTMRMFKEFFELPAEDKAIFYTEDARSKKHCKLYPSSLIYATEDVHLWR 131

Query: 176 DTLKHLTYPLEDVIQGWPDKPARYRDVMAKYSEEVRKMCHRILDLICEGLGLEDGFFRGE 355
           D LKH  +PLE  I  WP KP RYR V+  Y+ EV K+  RIL+LICEGLGLE G+F G+
Sbjct: 132 DNLKHDCHPLEKCILDWPVKPTRYRQVVGSYAAEVTKLVPRILELICEGLGLESGYFEGK 191

Query: 356 LSKGLGMVINNYPPCPEPSLAMGIHPHCDPYILTI-IQQQVYGLQIKRKDQWIGVEPLAN 532
           L +   + +N+YPPCP+PSLA+G+  HCDP ++TI +Q  V GLQI + ++WIGV+P+ N
Sbjct: 192 LGEVTSLSVNHYPPCPDPSLALGLSKHCDPNLITILLQDDVCGLQIFKDNEWIGVDPVPN 251

Query: 533 AFVIMIPYQLQVISNGKLSSCEHRGVTNSSTARISIVTFCGPTQECIIGPAKEHVSASNP 712
           AFV+ I YQLQ++SN KL S EHR VTNS TAR S   F  P+ + +I PAK  ++ASNP
Sbjct: 252 AFVVNIGYQLQIMSNNKLKSVEHRAVTNSRTARTSAAFFFNPSDDIMIAPAKALINASNP 311

Query: 713 PRFKPYKYLDFQINYL 760
           P F+  +Y++F  NY+
Sbjct: 312 PVFRACQYIEFLRNYM 327


>ref|XP_003632389.1| PREDICTED: LOW QUALITY PROTEIN: hyoscyamine 6-dioxygenase-like
           [Vitis vinifera]
          Length = 339

 Score =  274 bits (701), Expect = 2e-71
 Identities = 137/275 (49%), Positives = 185/275 (67%), Gaps = 3/275 (1%)
 Frame = +2

Query: 2   INHGVPDDLMQETISLYEEFFNLPVEVNENLLSDDFYKSVRLYTSGFNYANEEYHLWKDT 181
           INHGV D+L+ +++S+++EFFNLP E   +L S D  KS RLYTS F Y  EE H W D 
Sbjct: 71  INHGVSDNLIDDSMSIFKEFFNLPAEDKASLYSTDLNKSCRLYTSNFTYETEEVHFWSDI 130

Query: 182 LKHLTYPLEDVIQGWPDKPARYRDVMAKYSEEVRKMCHRILDLICEGLGLEDGFFRGELS 361
           L+H  +PL+D +Q WP+KP RYR+++  YS E+RK+  +ILDLICEGLGLE    +G LS
Sbjct: 131 LRHPCHPLQDQVQIWPEKPTRYREIVGAYSVELRKLSLKILDLICEGLGLE----QGXLS 186

Query: 362 KGLGMVINNYPPCPEPSLAMGIHPHCDPYILTII-QQQVYGLQIKRK--DQWIGVEPLAN 532
           KG  + +N+YP CP+PSL +G+  HCDP + T++ Q  V GLQ+ +    QW+GVEPL +
Sbjct: 187 KGQMLSVNHYPRCPDPSLTLGLPKHCDPNLKTVVLQGDVGGLQVYKDGIGQWLGVEPLPH 246

Query: 533 AFVIMIPYQLQVISNGKLSSCEHRGVTNSSTARISIVTFCGPTQECIIGPAKEHVSASNP 712
           AFV+ I YQLQ+ISNGKL   EHR +TN   AR +  TF  P+ +C+I PA+  V++SNP
Sbjct: 247 AFVVNIGYQLQIISNGKLRGAEHRVMTNPREARTTSATFINPSPDCVIHPAEALVNSSNP 306

Query: 713 PRFKPYKYLDFQINYLYHLSKKNLCSETALGPYLL 817
           P +K +KY+DF   Y           ET L P+ L
Sbjct: 307 PLYKAFKYVDFFGTYTAATGD----PETVLNPHKL 337


>gb|AAM48133.1|AF509338_1 putative flavanone 3-hydroxylase [Saussurea medusa]
           gi|48431269|gb|AAT44124.1| F3H-like protein [Saussurea
           medusa]
          Length = 343

 Score =  269 bits (687), Expect = 9e-70
 Identities = 136/273 (49%), Positives = 188/273 (68%), Gaps = 3/273 (1%)
 Frame = +2

Query: 2   INHGVPDDLMQETISLYEEFFNLPVEVNENLLSDDFYKS-VRLYTSGFNYANEEYHLWKD 178
           INHGV + +M++   LY EFFN+PV+       + +  +   LYTSG NYA E+ H WKD
Sbjct: 70  INHGVSEKMMEDMRVLYHEFFNMPVDDKLRGYFEPWSTTGCSLYTSGVNYAKEDVHYWKD 129

Query: 179 TLKHLTYPLEDVIQGWPDKPARYRDVMAKYSEEVRKMCHRILDLICEGLGLEDGFFRGEL 358
           TL+H  +PLE+    WP+KPARYR+ + +Y+ EVRKM  +ILDLI EGLGL +G+F G +
Sbjct: 130 TLRHPCHPLEEHTPSWPEKPARYREEVGRYAVEVRKMGFKILDLIGEGLGLNEGYFNG-V 188

Query: 359 SKGLGMVINNYPPCPEPSLAMGIHPHCDPYILTIIQQQVYGLQIKRKDQWIGVEPLANAF 538
           ++   M IN+YP CP+P LAMGI  H DP ++T +QQ  YGLQ+ +  +W+G++P+ NAF
Sbjct: 189 NQYQTMAINHYPLCPDPGLAMGIDGHTDPNLITFLQQDQYGLQMCKDGKWMGIDPIPNAF 248

Query: 539 VIMIPYQLQVISNGKLSSCEHRGVTNSSTARISIVTFCGPTQ--ECIIGPAKEHVSASNP 712
           V+ I YQL++ISNGKL S EHR VT+S+TAR SIVTF GP      ++ PAKE V++++P
Sbjct: 249 VVNIGYQLEIISNGKLKSAEHRVVTSSTTARTSIVTFFGPNAGLPIVVEPAKELVTSTSP 308

Query: 713 PRFKPYKYLDFQINYLYHLSKKNLCSETALGPY 811
             FK Y+Y +F  +YL ++ K    S T L PY
Sbjct: 309 KMFKSYQYNNFIADYLAYIRKPFPPSGTPLDPY 341


>ref|XP_002330260.1| predicted protein [Populus trichocarpa] gi|222871716|gb|EEF08847.1|
           predicted protein [Populus trichocarpa]
          Length = 327

 Score =  268 bits (684), Expect = 2e-69
 Identities = 124/257 (48%), Positives = 179/257 (69%), Gaps = 4/257 (1%)
 Frame = +2

Query: 2   INHGVPDDLMQETISLYEEFFNLPVEVNENLLSDDFYKS--VRLYTSGFNYANEEYHLWK 175
           INHGV +DLM +T+S+++EFF LP E   +   +D  ++    LYTS   YA E+ HLW+
Sbjct: 71  INHGVAEDLMNDTMSVFKEFFELPEEDKTSFYCEDAMRNNHCMLYTSSLLYATEDVHLWR 130

Query: 176 DTLKHLTYPLEDVIQGWPDKPARYRDVMAKYSEEVRKMCHRILDLICEGLGLEDGFFRGE 355
           D L++  +PLE+ IQ WP+KP RYR V+  Y+ EV+K+   IL+L+CEGLGLE G F G+
Sbjct: 131 DNLRNSCHPLEECIQHWPEKPTRYRHVVGAYATEVKKLAATILELLCEGLGLESGHFGGK 190

Query: 356 LSKGLGMVINNYPPCPEPSLAMGIHPHCDPYILTIIQQ--QVYGLQIKRKDQWIGVEPLA 529
           LS+   +++N+YPPCP+PSL +G+  HCDP ++TI+QQ   V+GLQ+ +  +WIGVEP++
Sbjct: 191 LSEIPSLLVNHYPPCPDPSLTLGVSEHCDPNLITILQQDSDVFGLQVLKNGEWIGVEPIS 250

Query: 530 NAFVIMIPYQLQVISNGKLSSCEHRGVTNSSTARISIVTFCGPTQECIIGPAKEHVSASN 709
           NAFV+ + YQ+Q++SN KL S EHR VTNS  AR S+  F  P  + I+ PAK  V   N
Sbjct: 251 NAFVVNMGYQMQIVSNNKLRSVEHRAVTNSEKARTSVAMFFIPNGDSIVEPAKALVDPRN 310

Query: 710 PPRFKPYKYLDFQINYL 760
           P  +K ++Y +F  ++L
Sbjct: 311 PAIYKSFQYKEFMSHFL 327