BLASTX nr result
ID: Bupleurum21_contig00032258
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00032258
(1127 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002273396.1| PREDICTED: probable pectinesterase/pectinest... 174 2e-54
emb|CBI29155.3| unnamed protein product [Vitis vinifera] 174 2e-54
ref|XP_003598363.1| Pectinesterase [Medicago truncatula] gi|3554... 169 1e-50
emb|CAN80988.1| hypothetical protein VITISV_008223 [Vitis vinifera] 172 5e-50
ref|XP_002515781.1| Pectinesterase-3 precursor, putative [Ricinu... 157 5e-49
>ref|XP_002273396.1| PREDICTED: probable pectinesterase/pectinesterase inhibitor 46-like
[Vitis vinifera]
Length = 564
Score = 174 bits (442), Expect(2) = 2e-54
Identities = 96/164 (58%), Positives = 118/164 (71%)
Frame = +1
Query: 508 VVAALKNCRELIDLATDHLNSSFLSDKSTFFEAVEDIVTWLSAAGTYQETCIDGFSNTTL 687
+ AL +C EL+DLA D+LNSS S F +D+ TWLSAAGTYQETCI+GF + L
Sbjct: 135 LAGALDDCYELLDLAIDNLNSSLSSSLDNF----DDLKTWLSAAGTYQETCINGFESGNL 190
Query: 688 RPNVLQYLKDSTEFTSNSLAIIKQISNAAKSLNLRRKLLTAADDTDMPHWLSFKDRKLLQ 867
R +VL++LK+STEF+SNSLAII +IS A S++ RR L + +P WLS KDRKLLQ
Sbjct: 191 RSSVLEFLKNSTEFSSNSLAIITEISKLAGSISSRR--LMGLPEDKVPKWLSAKDRKLLQ 248
Query: 868 SSKPSPIKFDAVVAKDRTGQYKTIGDALKAVPEKSKKRFTIYVK 999
SS K DAVVA D +G+YKTI +ALKAVP+KSKK F IYVK
Sbjct: 249 SSSTLKKKADAVVATDGSGKYKTISEALKAVPDKSKKSFVIYVK 292
Score = 65.9 bits (159), Expect(2) = 2e-54
Identities = 44/133 (33%), Positives = 64/133 (48%)
Frame = +2
Query: 80 MSLLKVYGKVDESDQDWFAGGRRKMMRKRXXXXXXXXXXXXXXXXXXXXXXXXXKRGXXX 259
MS K YGKV+E++Q+ R+ R+R G
Sbjct: 4 MSSFKAYGKVNEAEQERLLA--RRKTRRRITIISLSSIILVAVIVAAVVGTTTNSGGKSK 61
Query: 260 XXXXXXXXXEAASIKAACGVTLYPDSCFRSLAPLLNSSGRAIVQPETLYNASLQVAISEL 439
+ SIKA C VTLYPDSC+ SLAP++ SS ++PE L+N S++VA++EL
Sbjct: 62 NAGNSV----STSIKAVCDVTLYPDSCYNSLAPMVKSSQ---LKPEDLFNMSMEVALNEL 114
Query: 440 SNAAKYFVDMEGW 478
+ F + EG+
Sbjct: 115 HRTFQRFSEHEGF 127
>emb|CBI29155.3| unnamed protein product [Vitis vinifera]
Length = 561
Score = 174 bits (442), Expect(2) = 2e-54
Identities = 96/164 (58%), Positives = 118/164 (71%)
Frame = +1
Query: 508 VVAALKNCRELIDLATDHLNSSFLSDKSTFFEAVEDIVTWLSAAGTYQETCIDGFSNTTL 687
+ AL +C EL+DLA D+LNSS S F +D+ TWLSAAGTYQETCI+GF + L
Sbjct: 132 LAGALDDCYELLDLAIDNLNSSLSSSLDNF----DDLKTWLSAAGTYQETCINGFESGNL 187
Query: 688 RPNVLQYLKDSTEFTSNSLAIIKQISNAAKSLNLRRKLLTAADDTDMPHWLSFKDRKLLQ 867
R +VL++LK+STEF+SNSLAII +IS A S++ RR L + +P WLS KDRKLLQ
Sbjct: 188 RSSVLEFLKNSTEFSSNSLAIITEISKLAGSISSRR--LMGLPEDKVPKWLSAKDRKLLQ 245
Query: 868 SSKPSPIKFDAVVAKDRTGQYKTIGDALKAVPEKSKKRFTIYVK 999
SS K DAVVA D +G+YKTI +ALKAVP+KSKK F IYVK
Sbjct: 246 SSSTLKKKADAVVATDGSGKYKTISEALKAVPDKSKKSFVIYVK 289
Score = 65.9 bits (159), Expect(2) = 2e-54
Identities = 44/133 (33%), Positives = 64/133 (48%)
Frame = +2
Query: 80 MSLLKVYGKVDESDQDWFAGGRRKMMRKRXXXXXXXXXXXXXXXXXXXXXXXXXKRGXXX 259
MS K YGKV+E++Q+ R+ R+R G
Sbjct: 1 MSSFKAYGKVNEAEQERLLA--RRKTRRRITIISLSSIILVAVIVAAVVGTTTNSGGKSK 58
Query: 260 XXXXXXXXXEAASIKAACGVTLYPDSCFRSLAPLLNSSGRAIVQPETLYNASLQVAISEL 439
+ SIKA C VTLYPDSC+ SLAP++ SS ++PE L+N S++VA++EL
Sbjct: 59 NAGNSV----STSIKAVCDVTLYPDSCYNSLAPMVKSSQ---LKPEDLFNMSMEVALNEL 111
Query: 440 SNAAKYFVDMEGW 478
+ F + EG+
Sbjct: 112 HRTFQRFSEHEGF 124
>ref|XP_003598363.1| Pectinesterase [Medicago truncatula] gi|355487411|gb|AES68614.1|
Pectinesterase [Medicago truncatula]
Length = 574
Score = 169 bits (429), Expect(2) = 1e-50
Identities = 97/182 (53%), Positives = 124/182 (68%), Gaps = 4/182 (2%)
Frame = +1
Query: 466 HGGLASLFAGNKTEVVAALKNCRELIDLATDHLNSSFLSDK--STFFEAVEDIVTWLSAA 639
HG L ++T ALKNCR L+DLA DHLN++ + + S+ + +D+ TWLSAA
Sbjct: 125 HGVFKKLIENSRTN--EALKNCRVLLDLAIDHLNNTLTASRENSSLHQVFDDLQTWLSAA 182
Query: 640 GTYQETCIDGFSNTT--LRPNVLQYLKDSTEFTSNSLAIIKQISNAAKSLNLRRKLLTAA 813
GTYQ+TCI+GF +T L+ +V YLK+STE+TSNSLAII I+ A +LNLRR L++
Sbjct: 183 GTYQQTCIEGFEDTKEQLKTSVTSYLKNSTEYTSNSLAIITYINKAINTLNLRR-LMSLP 241
Query: 814 DDTDMPHWLSFKDRKLLQSSKPSPIKFDAVVAKDRTGQYKTIGDALKAVPEKSKKRFTIY 993
+ + P W KDRKLL S+K K D VVAKD +G+YKTI DALK VP KSKKR IY
Sbjct: 242 YENETPKWFHSKDRKLL-STKDLRSKADIVVAKDGSGKYKTISDALKHVPNKSKKRTLIY 300
Query: 994 VK 999
VK
Sbjct: 301 VK 302
Score = 57.8 bits (138), Expect(2) = 1e-50
Identities = 42/127 (33%), Positives = 54/127 (42%)
Frame = +2
Query: 80 MSLLKVYGKVDESDQDWFAGGRRKMMRKRXXXXXXXXXXXXXXXXXXXXXXXXXKRGXXX 259
MS K YGKVDE DQ R+ RKR K
Sbjct: 1 MSFFKSYGKVDEHDQMMLEAKRKT--RKRITIISLSSIIFIGVVIAAVLGVVNSKNDDDN 58
Query: 260 XXXXXXXXXEAASIKAACGVTLYPDSCFRSLAPLLNSSGRAIVQPETLYNASLQVAISEL 439
S+KA C VTLY DSC+ SL+ ++ S VQPE L+ S+ VA++ +
Sbjct: 59 NNNASQNSV-TNSMKAVCDVTLYKDSCYNSLSSIVGSGKE--VQPEELFKLSINVALTHV 115
Query: 440 SNAAKYF 460
S A +YF
Sbjct: 116 SKAVEYF 122
>emb|CAN80988.1| hypothetical protein VITISV_008223 [Vitis vinifera]
Length = 559
Score = 172 bits (437), Expect(2) = 5e-50
Identities = 95/164 (57%), Positives = 117/164 (71%)
Frame = +1
Query: 508 VVAALKNCRELIDLATDHLNSSFLSDKSTFFEAVEDIVTWLSAAGTYQETCIDGFSNTTL 687
+ AL +C EL+DLA D+LNSS S F +D+ TWLSAAGTYQETCI+GF + L
Sbjct: 121 LAGALDDCYELLDLAIDNLNSSLSSSLDNF----DDLKTWLSAAGTYQETCINGFESGNL 176
Query: 688 RPNVLQYLKDSTEFTSNSLAIIKQISNAAKSLNLRRKLLTAADDTDMPHWLSFKDRKLLQ 867
R +VL++LK+STEF+SNSLAII +IS S++ RR L + +P WLS KDRKLLQ
Sbjct: 177 RSSVLEFLKNSTEFSSNSLAIITEISKLXGSISSRR--LMGLPEDKVPKWLSAKDRKLLQ 234
Query: 868 SSKPSPIKFDAVVAKDRTGQYKTIGDALKAVPEKSKKRFTIYVK 999
SS K DAVVA D +G+YKTI +ALKAVP+KSKK F IYVK
Sbjct: 235 SSSTLKKKADAVVATDGSGKYKTISEALKAVPDKSKKSFVIYVK 278
Score = 52.8 bits (125), Expect(2) = 5e-50
Identities = 37/114 (32%), Positives = 51/114 (44%)
Frame = +2
Query: 80 MSLLKVYGKVDESDQDWFAGGRRKMMRKRXXXXXXXXXXXXXXXXXXXXXXXXXKRGXXX 259
MS K YGKV+E++Q+ R+ R+R G
Sbjct: 1 MSSFKAYGKVNEAEQERLLAXRKT--RRRITIISLSSIILVAVIVAAVVGTTTNSGGKSK 58
Query: 260 XXXXXXXXXEAASIKAACGVTLYPDSCFRSLAPLLNSSGRAIVQPETLYNASLQ 421
+ SIKA C VTLYPDSC+ SLAP++ SS ++PE L+N + Q
Sbjct: 59 NAGNSV----STSIKAVCDVTLYPDSCYNSLAPMVKSSQ---LKPEDLFNITFQ 105
>ref|XP_002515781.1| Pectinesterase-3 precursor, putative [Ricinus communis]
gi|223545109|gb|EEF46620.1| Pectinesterase-3 precursor,
putative [Ricinus communis]
Length = 573
Score = 157 bits (396), Expect(2) = 5e-49
Identities = 90/170 (52%), Positives = 114/170 (67%), Gaps = 3/170 (1%)
Frame = +1
Query: 514 AALKNCRELIDLATDHLNSSFLSDKS-TFFEAVEDIVTWLSAAGTYQETCIDGFSNTTLR 690
AALK+C++L+DLA D LNSS S + + +D +WLSAAG+YQ+TCIDG L+
Sbjct: 138 AALKDCQDLLDLAIDRLNSSLSSANDVSLIDVADDFRSWLSAAGSYQQTCIDGLKEANLK 197
Query: 691 PNVLQY-LKDSTEFTSNSLAIIKQISNAAKSLNLRRKLLTAADD-TDMPHWLSFKDRKLL 864
Y LK++TE TSNSLAII I A S+ +RR + A D ++P WL DRKLL
Sbjct: 198 STAQNYYLKNTTELTSNSLAIITWIYKIASSVKMRRLMSYAEHDKVNLPRWLHQNDRKLL 257
Query: 865 QSSKPSPIKFDAVVAKDRTGQYKTIGDALKAVPEKSKKRFTIYVKLQTHT 1014
QS+ K +AVVAKD +G+YKTI DALKAVP+KSKKRF IYVK +T
Sbjct: 258 QSNDLKK-KANAVVAKDGSGKYKTISDALKAVPDKSKKRFIIYVKKGIYT 306
Score = 65.1 bits (157), Expect(2) = 5e-49
Identities = 44/129 (34%), Positives = 59/129 (45%)
Frame = +2
Query: 80 MSLLKVYGKVDESDQDWFAGGRRKMMRKRXXXXXXXXXXXXXXXXXXXXXXXXXKRGXXX 259
MS K YGKVDE++Q RR RKR G
Sbjct: 1 MSSFKAYGKVDEAEQATLEARRRT--RKRITIISLSSILLAAIVVAAVVGTHAS--GGNS 56
Query: 260 XXXXXXXXXEAASIKAACGVTLYPDSCFRSLAPLLNSSGRAIVQPETLYNASLQVAISEL 439
+ S+KA C VTLY DSC+ SLAP + +QPE L+ S+QVA+ E+
Sbjct: 57 NNGGDQAKPISTSVKAVCDVTLYKDSCYSSLAPFAKPNN---LQPEELFKLSIQVALDEI 113
Query: 440 SNAAKYFVD 466
S A++YF++
Sbjct: 114 SKASQYFIN 122