BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00032224
         (469 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002521729.1| pentatricopeptide repeat-containing protein,...   228   3e-58
ref|XP_002307219.1| predicted protein [Populus trichocarpa] gi|2...   228   5e-58
ref|XP_002274224.2| PREDICTED: pentatricopeptide repeat-containi...   226   2e-57
emb|CBI30973.3| unnamed protein product [Vitis vinifera]              226   2e-57
ref|XP_003516519.1| PREDICTED: pentatricopeptide repeat-containi...   219   2e-55

>ref|XP_002521729.1| pentatricopeptide repeat-containing protein, putative [Ricinus
           communis] gi|223539120|gb|EEF40716.1| pentatricopeptide
           repeat-containing protein, putative [Ricinus communis]
          Length = 629

 Score =  228 bits (582), Expect = 3e-58
 Identities = 108/155 (69%), Positives = 130/155 (83%)
 Frame = -3

Query: 467 LMSEMQDPSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGR 288
           LM E+++P+ VTFN++I+AYC EENLVQALV+LEK F  G VPDV+T+TKVVEILC+ GR
Sbjct: 235 LMDEIEEPNDVTFNVLIAAYCKEENLVQALVLLEKSFSLGFVPDVVTMTKVVEILCNAGR 294

Query: 287 AMEAIEVVETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNI 108
             EA+E++E VE KGG+VDVVAYNTL++GF   GK+KV HRFLKEMERKGCLPN +TYNI
Sbjct: 295 VTEAVEMLERVEYKGGLVDVVAYNTLLRGFCRLGKIKVAHRFLKEMERKGCLPNVETYNI 354

Query: 107 LISGFCELGMLDSASDMFNEMKMVGINQNFITYDT 3
           LISGFC+ GM D A DMFN+MK  GI+ NF TYDT
Sbjct: 355 LISGFCDSGMFDMALDMFNDMKTDGISWNFDTYDT 389



 Score = 72.8 bits (177), Expect = 3e-11
 Identities = 39/148 (26%), Positives = 70/148 (47%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P  V  ++ I  +C +  +  A ++ ++    G  P+++    +V   C  G   EA E+
Sbjct: 452 PRAVDRSLRILGFCEKGAVKNAKMVFDQMINEGGTPNILVYDCLVHGFCQEGNLREAFEL 511

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGFCE 87
           +  +   G  +    +N LI GF   GK +   + L +M  +GC+P+  TY+ LI   C 
Sbjct: 512 MNEMVGHGYFLVASGFNALIHGFCGQGKDESALKLLDDMVGRGCVPDRGTYSPLIDALCR 571

Query: 86  LGMLDSASDMFNEMKMVGINQNFITYDT 3
            G    A  +FN+M   GI  +  T+++
Sbjct: 572 KGNFQKALSIFNQMIEKGITPDSSTWNS 599



 Score = 60.1 bits (144), Expect = 2e-07
 Identities = 43/149 (28%), Positives = 73/149 (48%), Gaps = 2/149 (1%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P+  T+NI+IS +C       AL M       G+  +  T   +++ L   GR  E +++
Sbjct: 347 PNVETYNILISGFCDSGMFDMALDMFNDMKTDGISWNFDTYDTLIKGLFFGGRIEEGLKI 406

Query: 266 VETVEK-KGGIVDVVA-YNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGF 93
           +E +E+ KGG    ++ YN+++ G            FL +ME+    P A   ++ I GF
Sbjct: 407 LELMEESKGGSGGRISPYNSVLYGLYKKNMWDEALEFLMKMEK--LFPRAVDRSLRILGF 464

Query: 92  CELGMLDSASDMFNEMKMVGINQNFITYD 6
           CE G + +A  +F++M   G   N + YD
Sbjct: 465 CEKGAVKNAKMVFDQMINEGGTPNILVYD 493


>ref|XP_002307219.1| predicted protein [Populus trichocarpa] gi|222856668|gb|EEE94215.1|
           predicted protein [Populus trichocarpa]
          Length = 584

 Score =  228 bits (580), Expect = 5e-58
 Identities = 108/155 (69%), Positives = 130/155 (83%)
 Frame = -3

Query: 467 LMSEMQDPSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGR 288
           LM+E+++P+ VTFN++I  YC E+N VQALV+LEK F  G VPDV+T+TKVVEILC+ GR
Sbjct: 211 LMNEIKEPNDVTFNVLICGYCKEDNFVQALVLLEKSFSLGFVPDVVTVTKVVEILCNVGR 270

Query: 287 AMEAIEVVETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNI 108
             EA+E++E VE KGG+VDVVA+NTLIKGF   GKVK+GH  LKEMERKGCLPNADTYN 
Sbjct: 271 VTEAVEILERVESKGGVVDVVAHNTLIKGFCKFGKVKLGHGLLKEMERKGCLPNADTYNA 330

Query: 107 LISGFCELGMLDSASDMFNEMKMVGINQNFITYDT 3
           LISGFCE GML+SA DMFN+MK  GIN NF+T+DT
Sbjct: 331 LISGFCESGMLESALDMFNDMKTDGINCNFVTFDT 365



 Score = 65.1 bits (157), Expect = 6e-09
 Identities = 43/160 (26%), Positives = 77/160 (48%), Gaps = 6/160 (3%)
 Frame = -3

Query: 467 LMSEMQD----PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILC 300
           L+ EM+     P+  T+N +IS +C    L  AL M       G+  + +T   +++ L 
Sbjct: 312 LLKEMERKGCLPNADTYNALISGFCESGMLESALDMFNDMKTDGINCNFVTFDTLIKGLF 371

Query: 299 SRGRAMEAIEVVETVEKKGGIV--DVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPN 126
           SRGR  +  +++E +E+  G+    +  YN+++ G      +     FL +ME     P 
Sbjct: 372 SRGRTEDGFKILELMEETRGVCGGSISPYNSVLYGLYRKNMLNEALEFLMKME--NLFPR 429

Query: 125 ADTYNILISGFCELGMLDSASDMFNEMKMVGINQNFITYD 6
           A   ++ I GFCE G +  A  ++++M   G   + + YD
Sbjct: 430 AVDRSLRILGFCEEGAIQEAKRVYDQMNNEGGIPSALVYD 469



 Score = 63.9 bits (154), Expect = 1e-08
 Identities = 41/129 (31%), Positives = 59/129 (45%)
 Frame = -3

Query: 431 FNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEVVETVE 252
           +N ++     +  L +AL  L K     L P  +  +  +   C  G   EA  V + + 
Sbjct: 400 YNSVLYGLYRKNMLNEALEFLMKM--ENLFPRAVDRSLRILGFCEEGAIQEAKRVYDQMN 457

Query: 251 KKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGFCELGMLD 72
            +GGI   + Y+ LI GF   G V+     + EM   G  P A T+N LISGFC LG   
Sbjct: 458 NEGGIPSALVYDCLIHGFCQEGCVREALELMNEMVFLGYFPVASTFNALISGFCRLGKDG 517

Query: 71  SASDMFNEM 45
           SA  +  +M
Sbjct: 518 SALKLLEDM 526



 Score = 61.6 bits (148), Expect = 6e-08
 Identities = 34/148 (22%), Positives = 68/148 (45%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P  V  ++ I  +C E  + +A  + ++    G +P  +    ++   C  G   EA+E+
Sbjct: 428 PRAVDRSLRILGFCEEGAIQEAKRVYDQMNNEGGIPSALVYDCLIHGFCQEGCVREALEL 487

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGFCE 87
           +  +   G       +N LI GF   GK     + L++M  +GC+P+  +Y+ LI   C 
Sbjct: 488 MNEMVFLGYFPVASTFNALISGFCRLGKDGSALKLLEDMVGRGCVPDTGSYSPLIDALCR 547

Query: 86  LGMLDSASDMFNEMKMVGINQNFITYDT 3
                 A+ +  +M   GI  +++ +++
Sbjct: 548 KKSFQKAASLLLQMVENGITPDYLIWNS 575


>ref|XP_002274224.2| PREDICTED: pentatricopeptide repeat-containing protein At2g17525,
           mitochondrial-like [Vitis vinifera]
          Length = 686

 Score =  226 bits (576), Expect = 2e-57
 Identities = 110/155 (70%), Positives = 125/155 (80%)
 Frame = -3

Query: 467 LMSEMQDPSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGR 288
           LM+EM +PS VTFN++ISAYC EENLVQALV+LEK F  G VPDV+T TKVV ILC  GR
Sbjct: 290 LMNEMVEPSDVTFNVLISAYCQEENLVQALVLLEKSFSMGFVPDVVTATKVVGILCKAGR 349

Query: 287 AMEAIEVVETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNI 108
             E +EV+E VE  GG+VDVVAYNTLIKGF   GK KVGHR LK+ME KGCLPN DTYNI
Sbjct: 350 VTEGVEVLERVESMGGVVDVVAYNTLIKGFCMLGKAKVGHRVLKDMEIKGCLPNVDTYNI 409

Query: 107 LISGFCELGMLDSASDMFNEMKMVGINQNFITYDT 3
           L SG+C+ GMLDSA D+FN+MK  GIN NF+TYDT
Sbjct: 410 LASGYCDSGMLDSAIDLFNDMKTDGINWNFMTYDT 444



 Score = 70.5 bits (171), Expect = 1e-10
 Identities = 37/148 (25%), Positives = 69/148 (46%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P  V  ++ I  +C E ++  A  + ++  K G VP V+    ++   C  G   EA E+
Sbjct: 507 PRAVDRSLRILGFCNEGSIGDAKRVYDQMIKEGGVPSVLVYVCLIHGFCQDGNVREAFEL 566

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGFCE 87
           +  +   G       +N LI  F   GKV    + +++M  +GCLP+  +Y+ ++   C 
Sbjct: 567 INEMVDHGYFPTAPTFNALISAFCGQGKVGSALKLMEDMVGRGCLPDMGSYSPMVDALCN 626

Query: 86  LGMLDSASDMFNEMKMVGINQNFITYDT 3
            G    A  +F +M    I  ++ T+++
Sbjct: 627 KGDFQKAVRLFLQMVEKDILPDYSTWNS 654



 Score = 58.5 bits (140), Expect = 5e-07
 Identities = 38/148 (25%), Positives = 70/148 (47%), Gaps = 2/148 (1%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P+  T+NI+ S YC    L  A+ +       G+  + +T   ++  LCS GR  +  ++
Sbjct: 402 PNVDTYNILASGYCDSGMLDSAIDLFNDMKTDGINWNFMTYDTLIRGLCSGGRMEDGFKI 461

Query: 266 VETVEKKGGIVD--VVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGF 93
           +E +E+  G     +  YN++I G     + +    FL +ME+    P A   ++ I GF
Sbjct: 462 LELMEESRGGAGGRISPYNSIIYGLYKKNQFEEALEFLTKMEK--LFPRAVDRSLRILGF 519

Query: 92  CELGMLDSASDMFNEMKMVGINQNFITY 9
           C  G +  A  ++++M   G   + + Y
Sbjct: 520 CNEGSIGDAKRVYDQMIKEGGVPSVLVY 547



 Score = 57.8 bits (138), Expect = 9e-07
 Identities = 25/115 (21%), Positives = 58/115 (50%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           PS + +  +I  +C + N+ +A  ++ +   HG  P   T   ++   C +G+   A+++
Sbjct: 542 PSVLVYVCLIHGFCQDGNVREAFELINEMVDHGYFPTAPTFNALISAFCGQGKVGSALKL 601

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILI 102
           +E +  +G + D+ +Y+ ++    + G  +   R   +M  K  LP+  T+N ++
Sbjct: 602 MEDMVGRGCLPDMGSYSPMVDALCNKGDFQKAVRLFLQMVEKDILPDYSTWNSML 656


>emb|CBI30973.3| unnamed protein product [Vitis vinifera]
          Length = 722

 Score =  226 bits (576), Expect = 2e-57
 Identities = 110/155 (70%), Positives = 125/155 (80%)
 Frame = -3

Query: 467 LMSEMQDPSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGR 288
           LM+EM +PS VTFN++ISAYC EENLVQALV+LEK F  G VPDV+T TKVV ILC  GR
Sbjct: 138 LMNEMVEPSDVTFNVLISAYCQEENLVQALVLLEKSFSMGFVPDVVTATKVVGILCKAGR 197

Query: 287 AMEAIEVVETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNI 108
             E +EV+E VE  GG+VDVVAYNTLIKGF   GK KVGHR LK+ME KGCLPN DTYNI
Sbjct: 198 VTEGVEVLERVESMGGVVDVVAYNTLIKGFCMLGKAKVGHRVLKDMEIKGCLPNVDTYNI 257

Query: 107 LISGFCELGMLDSASDMFNEMKMVGINQNFITYDT 3
           L SG+C+ GMLDSA D+FN+MK  GIN NF+TYDT
Sbjct: 258 LASGYCDSGMLDSAIDLFNDMKTDGINWNFMTYDT 292



 Score = 70.5 bits (171), Expect = 1e-10
 Identities = 37/148 (25%), Positives = 69/148 (46%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P  V  ++ I  +C E ++  A  + ++  K G VP V+    ++   C  G   EA E+
Sbjct: 355 PRAVDRSLRILGFCNEGSIGDAKRVYDQMIKEGGVPSVLVYVCLIHGFCQDGNVREAFEL 414

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGFCE 87
           +  +   G       +N LI  F   GKV    + +++M  +GCLP+  +Y+ ++   C 
Sbjct: 415 INEMVDHGYFPTAPTFNALISAFCGQGKVGSALKLMEDMVGRGCLPDMGSYSPMVDALCN 474

Query: 86  LGMLDSASDMFNEMKMVGINQNFITYDT 3
            G    A  +F +M    I  ++ T+++
Sbjct: 475 KGDFQKAVRLFLQMVEKDILPDYSTWNS 502



 Score = 58.5 bits (140), Expect = 5e-07
 Identities = 38/148 (25%), Positives = 70/148 (47%), Gaps = 2/148 (1%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P+  T+NI+ S YC    L  A+ +       G+  + +T   ++  LCS GR  +  ++
Sbjct: 250 PNVDTYNILASGYCDSGMLDSAIDLFNDMKTDGINWNFMTYDTLIRGLCSGGRMEDGFKI 309

Query: 266 VETVEKKGGIVD--VVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGF 93
           +E +E+  G     +  YN++I G     + +    FL +ME+    P A   ++ I GF
Sbjct: 310 LELMEESRGGAGGRISPYNSIIYGLYKKNQFEEALEFLTKMEK--LFPRAVDRSLRILGF 367

Query: 92  CELGMLDSASDMFNEMKMVGINQNFITY 9
           C  G +  A  ++++M   G   + + Y
Sbjct: 368 CNEGSIGDAKRVYDQMIKEGGVPSVLVY 395



 Score = 57.8 bits (138), Expect = 9e-07
 Identities = 25/115 (21%), Positives = 58/115 (50%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           PS + +  +I  +C + N+ +A  ++ +   HG  P   T   ++   C +G+   A+++
Sbjct: 390 PSVLVYVCLIHGFCQDGNVREAFELINEMVDHGYFPTAPTFNALISAFCGQGKVGSALKL 449

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILI 102
           +E +  +G + D+ +Y+ ++    + G  +   R   +M  K  LP+  T+N ++
Sbjct: 450 MEDMVGRGCLPDMGSYSPMVDALCNKGDFQKAVRLFLQMVEKDILPDYSTWNSML 504


>ref|XP_003516519.1| PREDICTED: pentatricopeptide repeat-containing protein At2g17525,
           mitochondrial-like [Glycine max]
          Length = 618

 Score =  219 bits (558), Expect = 2e-55
 Identities = 106/155 (68%), Positives = 126/155 (81%)
 Frame = -3

Query: 467 LMSEMQDPSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGR 288
           LM+EM+DP+ VTFNI+IS YC E N VQALV+LEK F  G VPDV+++TKV+EILC+ GR
Sbjct: 232 LMNEMEDPNDVTFNILISGYCKEGNSVQALVLLEKSFSMGFVPDVVSVTKVLEILCNAGR 291

Query: 287 AMEAIEVVETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNI 108
            MEA EV+E VE  GG++DVVAYNTLIKGF  +GKVKVG  FLK+ME KGCLPN DTYN+
Sbjct: 292 TMEAAEVLERVESMGGLLDVVAYNTLIKGFCGAGKVKVGLHFLKQMENKGCLPNVDTYNV 351

Query: 107 LISGFCELGMLDSASDMFNEMKMVGINQNFITYDT 3
           LISGF E GMLD A D+FN+MK  GI  NF+T+DT
Sbjct: 352 LISGFSESGMLDLALDLFNDMKTDGIKWNFVTFDT 386



 Score = 67.4 bits (163), Expect = 1e-09
 Identities = 34/113 (30%), Positives = 56/113 (49%)
 Frame = -3

Query: 347 LVPDVITLTKVVEILCSRGRAMEAIEVVETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGH 168
           L P  +  + ++   C +G   +A  V + +  +GGI  ++ YN L+ GFS  G V+   
Sbjct: 447 LFPRAVDRSLMILEHCKKGAIEDAKRVYDQMIDEGGIPSILVYNCLVHGFSKQGNVREAV 506

Query: 167 RFLKEMERKGCLPNADTYNILISGFCELGMLDSASDMFNEMKMVGINQNFITY 9
             + EM    C P   T+N +I+GFC  G ++SA  +  ++   G   N  TY
Sbjct: 507 ELMNEMIANNCFPIPSTFNAVITGFCRQGKVESALKLVEDITARGCVPNTETY 559



 Score = 66.2 bits (160), Expect = 3e-09
 Identities = 36/139 (25%), Positives = 67/139 (48%)
 Frame = -3

Query: 446 PSQVTFNIMISAYCGEENLVQALVMLEKCFKHGLVPDVITLTKVVEILCSRGRAMEAIEV 267
           P  V  ++MI  +C +  +  A  + ++    G +P ++    +V     +G   EA+E+
Sbjct: 449 PRAVDRSLMILEHCKKGAIEDAKRVYDQMIDEGGIPSILVYNCLVHGFSKQGNVREAVEL 508

Query: 266 VETVEKKGGIVDVVAYNTLIKGFSSSGKVKVGHRFLKEMERKGCLPNADTYNILISGFCE 87
           +  +           +N +I GF   GKV+   + ++++  +GC+PN +TY+ LI   C 
Sbjct: 509 MNEMIANNCFPIPSTFNAVITGFCRQGKVESALKLVEDITARGCVPNTETYSPLIDVLCR 568

Query: 86  LGMLDSASDMFNEMKMVGI 30
            G L  A  +F +M   GI
Sbjct: 569 NGDLQKAMQVFMQMVDKGI 587