BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00031466
         (1347 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267626.2| PREDICTED: vacuolar protein sorting-associat...   488   e-135
emb|CBI38711.3| unnamed protein product [Vitis vinifera]              488   e-135
ref|XP_002320230.1| predicted protein [Populus trichocarpa] gi|2...   477   e-132
ref|XP_002515073.1| conserved hypothetical protein [Ricinus comm...   455   e-125
ref|XP_003529870.1| PREDICTED: vacuolar protein sorting-associat...   447   e-123

>ref|XP_002267626.2| PREDICTED: vacuolar protein sorting-associated protein 8 homolog
            [Vitis vinifera]
          Length = 1979

 Score =  488 bits (1257), Expect = e-135
 Identities = 271/463 (58%), Positives = 325/463 (70%), Gaps = 14/463 (3%)
 Frame = -1

Query: 1347 LDLAEELEKKNALIGSPQENGAASQPTRLEGVYPGSTALGYFDIDSNNMVTRTISSETFR 1168
            L+LAEELEK  A  G   E GAA+QP RLEGV  GST LGYF+ID+NN +TRTISS  F+
Sbjct: 413  LELAEELEKSQASTGLHWEEGAAAQPMRLEGVRRGSTTLGYFEIDNNNTITRTISSPAFK 472

Query: 1167 RDHGFAQVLAVHHNFIAVGMSKGVIFRFPTKKTLPNAES-DAKIVMFGTQGDRSHASVMS 991
            RDHG  QVLAVH NFIAVGMS+GV+   P+K +  NA++ DAKI+M G QG+RSHA V S
Sbjct: 473  RDHGSPQVLAVHLNFIAVGMSRGVVMVVPSKYSAYNADNMDAKILMLGLQGERSHAPVTS 532

Query: 990  MCFNRQGDLLFAGYSDGRYTIWDVQKATSLKTVR-EHKAPVVHIFYLGLDSQASRQFNVV 814
            MCFN QGDLL AGY DG  T+WDVQ+AT+ K +  EH APV+H  +LG DSQ +RQF  V
Sbjct: 533  MCFNHQGDLLLAGYGDGHITVWDVQRATAAKVITGEHSAPVIHTLFLGQDSQVTRQFKAV 592

Query: 813  SGDSKGVIKMIHFSTVLWVN---MTKQVILLDEKTSTVICASALLSDEYXXXXXXXXXXX 643
            +GDSKG++ +  FS V  +N   +  Q +L  ++T TV+ AS LL DE            
Sbjct: 593  TGDSKGLVLLHAFSVVPLLNRFSIKTQCLLDGQRTGTVLSASPLLLDESSGSSLMSSQGN 652

Query: 642  XXXXXXXXXXXXXXXXXGD--WKLF-EGSSLVEEGVVIFVSHQSALVAKVNPSVEVYAQL 472
                             GD  WKLF EGSSLVEEGVVIFV+HQ+ALV +++PS+EVYAQL
Sbjct: 653  ATGSTSSIGSMMGGVVGGDAGWKLFSEGSSLVEEGVVIFVTHQTALVVRLSPSLEVYAQL 712

Query: 471  PKPESVREGCMPYAAWKYTRQPCTTYNG------NVPEETSERVGLLALAWDLEVQVAGL 310
             KP+ VREG MPY AWK     C T +       N P E SERV LLA+AWD +VQVA L
Sbjct: 713  NKPDGVREGSMPYTAWK-----CMTIHSRGLSTENTPVEASERVSLLAIAWDRKVQVAKL 767

Query: 309  VKSKLKIHRKLTLKSSAVGVAWLDDQLLVVLTSDGQLCLFETEEGTMIHQTSFAVDRFRG 130
            VKS+LKI+ K TL+S+A+GVAWLDDQ+LVVLTS GQLCLF  ++GT+IHQTSFAVD   G
Sbjct: 768  VKSELKIYGKWTLESTAIGVAWLDDQILVVLTSTGQLCLF-AKDGTVIHQTSFAVDGSGG 826

Query: 129  DDLIACHDLFINTFGNPEKAYHNSVAISGASIYILGPMQLTVS 1
            DD +A H  F N FGNPEKAY NS+A+ GASIYILGP+ L VS
Sbjct: 827  DDPVAYHTYFTNIFGNPEKAYQNSIAVRGASIYILGPVHLVVS 869


>emb|CBI38711.3| unnamed protein product [Vitis vinifera]
          Length = 1934

 Score =  488 bits (1257), Expect = e-135
 Identities = 271/463 (58%), Positives = 325/463 (70%), Gaps = 14/463 (3%)
 Frame = -1

Query: 1347 LDLAEELEKKNALIGSPQENGAASQPTRLEGVYPGSTALGYFDIDSNNMVTRTISSETFR 1168
            L+LAEELEK  A  G   E GAA+QP RLEGV  GST LGYF+ID+NN +TRTISS  F+
Sbjct: 368  LELAEELEKSQASTGLHWEEGAAAQPMRLEGVRRGSTTLGYFEIDNNNTITRTISSPAFK 427

Query: 1167 RDHGFAQVLAVHHNFIAVGMSKGVIFRFPTKKTLPNAES-DAKIVMFGTQGDRSHASVMS 991
            RDHG  QVLAVH NFIAVGMS+GV+   P+K +  NA++ DAKI+M G QG+RSHA V S
Sbjct: 428  RDHGSPQVLAVHLNFIAVGMSRGVVMVVPSKYSAYNADNMDAKILMLGLQGERSHAPVTS 487

Query: 990  MCFNRQGDLLFAGYSDGRYTIWDVQKATSLKTVR-EHKAPVVHIFYLGLDSQASRQFNVV 814
            MCFN QGDLL AGY DG  T+WDVQ+AT+ K +  EH APV+H  +LG DSQ +RQF  V
Sbjct: 488  MCFNHQGDLLLAGYGDGHITVWDVQRATAAKVITGEHSAPVIHTLFLGQDSQVTRQFKAV 547

Query: 813  SGDSKGVIKMIHFSTVLWVN---MTKQVILLDEKTSTVICASALLSDEYXXXXXXXXXXX 643
            +GDSKG++ +  FS V  +N   +  Q +L  ++T TV+ AS LL DE            
Sbjct: 548  TGDSKGLVLLHAFSVVPLLNRFSIKTQCLLDGQRTGTVLSASPLLLDESSGSSLMSSQGN 607

Query: 642  XXXXXXXXXXXXXXXXXGD--WKLF-EGSSLVEEGVVIFVSHQSALVAKVNPSVEVYAQL 472
                             GD  WKLF EGSSLVEEGVVIFV+HQ+ALV +++PS+EVYAQL
Sbjct: 608  ATGSTSSIGSMMGGVVGGDAGWKLFSEGSSLVEEGVVIFVTHQTALVVRLSPSLEVYAQL 667

Query: 471  PKPESVREGCMPYAAWKYTRQPCTTYNG------NVPEETSERVGLLALAWDLEVQVAGL 310
             KP+ VREG MPY AWK     C T +       N P E SERV LLA+AWD +VQVA L
Sbjct: 668  NKPDGVREGSMPYTAWK-----CMTIHSRGLSTENTPVEASERVSLLAIAWDRKVQVAKL 722

Query: 309  VKSKLKIHRKLTLKSSAVGVAWLDDQLLVVLTSDGQLCLFETEEGTMIHQTSFAVDRFRG 130
            VKS+LKI+ K TL+S+A+GVAWLDDQ+LVVLTS GQLCLF  ++GT+IHQTSFAVD   G
Sbjct: 723  VKSELKIYGKWTLESTAIGVAWLDDQILVVLTSTGQLCLF-AKDGTVIHQTSFAVDGSGG 781

Query: 129  DDLIACHDLFINTFGNPEKAYHNSVAISGASIYILGPMQLTVS 1
            DD +A H  F N FGNPEKAY NS+A+ GASIYILGP+ L VS
Sbjct: 782  DDPVAYHTYFTNIFGNPEKAYQNSIAVRGASIYILGPVHLVVS 824


>ref|XP_002320230.1| predicted protein [Populus trichocarpa] gi|222861003|gb|EEE98545.1|
            predicted protein [Populus trichocarpa]
          Length = 1976

 Score =  477 bits (1227), Expect = e-132
 Identities = 261/473 (55%), Positives = 325/473 (68%), Gaps = 24/473 (5%)
 Frame = -1

Query: 1347 LDLAEELEKKNALIGSPQENGAASQPTRLEGVYPGSTALGYFDIDSNNMVTRTISSETFR 1168
            L+LAEELEKK A  G   E GAA+QP RLEGV  GST+LGYFD+DS+N++T+T+ S+TFR
Sbjct: 367  LELAEELEKKMAYTGLHWEEGAAAQPMRLEGVRRGSTSLGYFDVDSHNVITQTVGSQTFR 426

Query: 1167 RDHGFAQVLAVHHNFIAVGMSKGVIFRFPTKKTLPNAES-----------------DAKI 1039
            RDHG  QVLAVH N+IAVGMSKGVI   P++ +  N ++                 D K+
Sbjct: 427  RDHGSPQVLAVHLNYIAVGMSKGVIVVVPSRYSSHNDDNMDAKWMSLPFVFLLLLKDGKM 486

Query: 1038 VMFGTQGDRSHASVMSMCFNRQGDLLFAGYSDGRYTIWDVQKATSLKTVR-EHKAPVVHI 862
            +M G QGDRSHA V SMCFN+QGD+L AGY DG  T+WDVQ+A++ K +  EH APVVH 
Sbjct: 487  LMLGLQGDRSHAPVTSMCFNQQGDMLLAGYGDGHITVWDVQRASAAKVITGEHTAPVVHA 546

Query: 861  FYLGLDSQASRQFNVVSGDSKGVIKMIHFSTVLWVN---MTKQVILLDEKTSTVICASAL 691
            F+LG DSQ +RQF  V+GDSKG++ +  FS V  +N      Q +L  ++T TV+ AS L
Sbjct: 547  FFLGQDSQVTRQFKAVTGDSKGLVLLHAFSVVPLLNRFSFKTQCLLDGQRTGTVLSASPL 606

Query: 690  LSDEYXXXXXXXXXXXXXXXXXXXXXXXXXXXXGD--WKLF-EGSSLVEEGVVIFVSHQS 520
            L DE                             GD  WKLF EGSSLVEEGVVIFV+HQ+
Sbjct: 607  LLDESCGGALPATQGNSSASSTSISSMMGGVVGGDAGWKLFNEGSSLVEEGVVIFVTHQT 666

Query: 519  ALVAKVNPSVEVYAQLPKPESVREGCMPYAAWKYTRQPCTTYNGNVPEETSERVGLLALA 340
            ALV +++PS++VYAQL +P+ VREG MPY AWK T Q  ++   NVPE  +ERV LLA+A
Sbjct: 667  ALVVRLSPSLQVYAQLSRPDGVREGSMPYTAWKCTTQSHSSSPDNVPEHVAERVSLLAIA 726

Query: 339  WDLEVQVAGLVKSKLKIHRKLTLKSSAVGVAWLDDQLLVVLTSDGQLCLFETEEGTMIHQ 160
            WD +VQVA LVKS+LK++ K +L S+A+GVAWLDD +LVVLT  GQL LF  ++GT+IHQ
Sbjct: 727  WDRKVQVAKLVKSELKVYGKWSLDSAAIGVAWLDDHMLVVLTLTGQLYLF-AKDGTVIHQ 785

Query: 159  TSFAVDRFRGDDLIACHDLFINTFGNPEKAYHNSVAISGASIYILGPMQLTVS 1
            TSFAVD  RGDDL A H   IN +GNPEKAYHN + + GAS+YILGP  L VS
Sbjct: 786  TSFAVDGSRGDDLAAYHTHLINIYGNPEKAYHNCIGVRGASVYILGPTHLIVS 838


>ref|XP_002515073.1| conserved hypothetical protein [Ricinus communis]
            gi|223545553|gb|EEF47057.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 1899

 Score =  455 bits (1170), Expect = e-125
 Identities = 253/466 (54%), Positives = 314/466 (67%), Gaps = 17/466 (3%)
 Frame = -1

Query: 1347 LDLAEELEKKNALIGSPQENGAASQPTRLEGVYPGSTALGYFDIDSNNMVTRTISSETFR 1168
            L+LAEELEKK A  G   E GAA+QP +LEGV  GST LGYFDID+NN +TRTI S+TFR
Sbjct: 349  LELAEELEKKQASTGLHLEEGAAAQPMKLEGVRRGSTTLGYFDIDANNAITRTIMSQTFR 408

Query: 1167 RDHGFAQVLAVHHNFIAVGMSKGVIFRFPTKKTLPNAES-DAKIVMFGTQGDRSHASVMS 991
            RDHG  QVLAVH N IAVGM KGVI   P++ +  N ++ D+K++M G QGDRS+A V S
Sbjct: 409  RDHGSPQVLAVHLNHIAVGMGKGVIAVVPSRYSPYNGDNMDSKMLMLGLQGDRSYAPVTS 468

Query: 990  MCFNRQGDLLFAGYSDGRYTIWDVQKATSLKTVR-EHKAPVVHIFYLGLDSQASRQFNVV 814
            MCFN+QGDLL AGY DG  T+WD+Q+A+  K +  EH APVVH  +LG DSQ +RQF  V
Sbjct: 469  MCFNQQGDLLLAGYGDGHITVWDIQRASVAKVITGEHTAPVVHALFLGQDSQVTRQFKAV 528

Query: 813  SGDSKGVIKMIHFSTVLWVNMTK------------QVILLDEKTSTVICASALLSDEYXX 670
            +GDSKG + +  FS V  +N                 +L  ++T  V+ AS LL DE   
Sbjct: 529  TGDSKGHVYLHSFSVVPLLNRFTIKTQANPYSSLLHCLLDGQRTGIVLSASPLLFDESSG 588

Query: 669  XXXXXXXXXXXXXXXXXXXXXXXXXXGD--WKLF-EGSSLVEEGVVIFVSHQSALVAKVN 499
                                      GD  WKLF EGSS VEEGVVIFV+HQ+ALV ++ 
Sbjct: 589  GALPSSQGNASVSSSSIGNMMGGVVGGDAGWKLFNEGSSPVEEGVVIFVTHQTALVVRLT 648

Query: 498  PSVEVYAQLPKPESVREGCMPYAAWKYTRQPCTTYNGNVPEETSERVGLLALAWDLEVQV 319
            P++EVYAQL KP+ VREG MPY AWK T Q  ++   N+  + +ERV LLA+AWD +VQV
Sbjct: 649  PTLEVYAQLSKPDGVREGSMPYTAWKCTSQSHSSEYENISADAAERVSLLAVAWDRKVQV 708

Query: 318  AGLVKSKLKIHRKLTLKSSAVGVAWLDDQLLVVLTSDGQLCLFETEEGTMIHQTSFAVDR 139
            A L+KS+LK++   +L S+A+GV WLD  +LVVLT  GQL LF  ++GT+IHQTSFAVD 
Sbjct: 709  AKLIKSELKVYGTWSLDSAAIGVTWLDAHMLVVLTLTGQLYLF-AKDGTVIHQTSFAVDG 767

Query: 138  FRGDDLIACHDLFINTFGNPEKAYHNSVAISGASIYILGPMQLTVS 1
              GDDL+A H  FIN +GNPEKAYHNS+A+ GAS+YILGP  L VS
Sbjct: 768  SGGDDLVAYHTHFINIYGNPEKAYHNSLAVRGASVYILGPTHLVVS 813


>ref|XP_003529870.1| PREDICTED: vacuolar protein sorting-associated protein 8 homolog
            [Glycine max]
          Length = 1886

 Score =  447 bits (1150), Expect = e-123
 Identities = 246/458 (53%), Positives = 315/458 (68%), Gaps = 9/458 (1%)
 Frame = -1

Query: 1347 LDLAEELEKKNALIGSPQENGAASQPTRLEGVYPGSTALGYFDIDSNNMVTRTISSETFR 1168
            L+LAEELEKK A  G   E GAA+QP RLEGV  GST LGYFD+D+ N  TR ISS+TFR
Sbjct: 317  LELAEELEKKRASTGLHLEEGAAAQPMRLEGVRRGSTTLGYFDVDAGNAFTRAISSQTFR 376

Query: 1167 RDHGFAQVLAVHHNFIAVGMSKGVIFRFPTKKTLPNAE-SDAKIVMFGTQGDRSHASVMS 991
            R+ G  + LAVH N+IAVGMSKG+I  FP+K ++ +A+ SD K++M   QGDR HA V S
Sbjct: 377  REQGSTRALAVHANYIAVGMSKGLIVVFPSKYSIHHADNSDGKMMMLAIQGDRLHAPVTS 436

Query: 990  MCFNRQGDLLFAGYSDGRYTIWDVQKATSLKTVR-EHKAPVVHIFYLGLDSQASRQFNVV 814
            M FN+QGDLL AGY DG  T+WDVQK    K +  EH APVVH  +LG D Q +RQF  V
Sbjct: 437  MSFNQQGDLLLAGYGDGHLTLWDVQKGVVAKVISGEHTAPVVHTLFLGQDPQNTRQFKAV 496

Query: 813  SGDSKGVIKMIHFSTVLWV----NMTKQVILLDEKTSTVICASALLSDEYXXXXXXXXXX 646
            +GD KG++ + H  +V+ +    ++  Q +L  + T  V+ AS LL D++          
Sbjct: 497  TGDCKGLV-LFHIISVVPLFSRFSIKTQCLLDGQSTGLVLSASPLLFDDFSGSASPYTQG 555

Query: 645  XXXXXXXXXXXXXXXXXXGD--WKLF-EGSSLVEEGVVIFVSHQSALVAKVNPSVEVYAQ 475
                              GD  WKLF E  SLVEEGVV+FV+HQ+ALV +++P+++VYAQ
Sbjct: 556  NTSAPASSISSMMGGVVGGDAGWKLFNEAPSLVEEGVVVFVTHQTALVVRLSPTLQVYAQ 615

Query: 474  LPKPESVREGCMPYAAWKYTRQPCTTYNGNVPEETSERVGLLALAWDLEVQVAGLVKSKL 295
            L +P+ VREG MPY AWKY  Q C++   N+  E  ERV LLA+AW+ +V VA LVKS+L
Sbjct: 616  LSRPDGVREGSMPYTAWKYMTQICSS-TENMSAEAVERVSLLAIAWERKVLVAKLVKSEL 674

Query: 294  KIHRKLTLKSSAVGVAWLDDQLLVVLTSDGQLCLFETEEGTMIHQTSFAVDRFRGDDLIA 115
            K++ + +L  +A+G+AWLDDQ+LVVLTS GQL LF +++GT+IHQTSF+VD   GDDL++
Sbjct: 675  KVYGRWSLDGAALGLAWLDDQMLVVLTSSGQLYLF-SKDGTVIHQTSFSVDGIGGDDLVS 733

Query: 114  CHDLFINTFGNPEKAYHNSVAISGASIYILGPMQLTVS 1
             H  FIN FGNPEKAYHNSVA+ GASIYILGP  L VS
Sbjct: 734  YHTHFINIFGNPEKAYHNSVAVRGASIYILGPTHLLVS 771