BLASTX nr result
ID: Bupleurum21_contig00031288
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00031288
(1145 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003612608.1| Replication protein A 70 kDa DNA-binding sub... 172 1e-40
ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative rib... 159 1e-36
gb|ABD28710.1| Polynucleotidyl transferase, Ribonuclease H fold ... 158 3e-36
gb|ABN06084.1| Polynucleotidyl transferase, Ribonuclease H fold ... 152 2e-34
gb|ABD32189.2| Polynucleotidyl transferase, Ribonuclease H fold ... 152 2e-34
>ref|XP_003612608.1| Replication protein A 70 kDa DNA-binding subunit [Medicago
truncatula] gi|355513943|gb|AES95566.1| Replication
protein A 70 kDa DNA-binding subunit [Medicago
truncatula]
Length = 1723
Score = 172 bits (436), Expect = 1e-40
Identities = 105/329 (31%), Positives = 154/329 (46%), Gaps = 8/329 (2%)
Frame = +3
Query: 180 FWQKHFGQCNQGATHK---GWTKLWNLEVPQKVKVFLWRICRNNIPVRNLLRGRGVNTTI 350
F H Q +TH+ W LWNL+VP KVK FLWR CRN +P R L+ RGVN T
Sbjct: 1397 FEPNHLSQHVDLSTHRCDGNWVLLWNLKVPPKVKTFLWRSCRNALPTRVRLQDRGVNCTK 1456
Query: 351 LCPMCGSDVEHLLHIFHDCKFAKGCWRFFGL----NYNAGAIESCPEWVLHCLATESHEK 518
C +C ++ E +H+F C ++ CW+ GL A S + L +
Sbjct: 1457 TCALCENEDEDSMHLFFYCTKSRQCWQQLGLWSKVQQKAQLNLSFGVTMFEILQELDSSQ 1516
Query: 519 SIQMATVLWGIWVARNMIVWEQKPLTPELAMHWSARQIAQWREVQALKGSSIKGRTQGQQ 698
+ A V+W IW RN +W + +T + W+ Q +
Sbjct: 1517 RVIWACVMWSIWKQRNDCIWRNEVMTTAAVRDRGLNLLTGWQNAQDI------------- 1563
Query: 699 QSVVRWKAPEEGQFKINVDAAVQLGRPDFKVGMVLRDHRGNFCNARVACLGGEVTVFEAE 878
W+ P+EG FK NVDAA +G+ +RD G AR + + V E E
Sbjct: 1564 -----WRKPDEGHFKCNVDAAFFKESNRVGIGICIRDDSGRLVKARTSWSTLLLDVPEGE 1618
Query: 879 ARGMLEAVRWATELGISNVIFESDSMLTVQALNRKTINYLEVGNMLQDCRSLIND-RPDF 1055
A G+L A+RWA E ++N+ FE DS V + + + ++G ++++CR+ + +
Sbjct: 1619 AIGLLYAIRWAKEQNLNNITFELDSKRVVYSFHNTRNDVSDLGAIIRECRTTFSSFFTNS 1678
Query: 1056 SVTFVRRQANKVAHLLARVPCDAYCFHDF 1142
V F+RRQAN+V H LAR H F
Sbjct: 1679 RVEFIRRQANEVVHSLARAATFYASIHYF 1707
>ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative ribonuclease H protein
At1g65750-like [Vitis vinifera]
Length = 820
Score = 159 bits (402), Expect = 1e-36
Identities = 99/344 (28%), Positives = 150/344 (43%), Gaps = 5/344 (1%)
Frame = +3
Query: 120 DRIAWMHTIDGKYTVKSGYS----FWQKHFGQCNQGATHKGWTKLWNLEVPQKVKVFLWR 287
D+I+W G+YT +SGY F Q + ++ W +LW + P K+ F WR
Sbjct: 467 DQISWSFDARGEYTARSGYGALRCFRQSTALVASDVDSNFVWAQLWKVTAPPKILNFAWR 526
Query: 288 ICRNNIPVRNLLRGRGVNTTILCPMCGSDVEHLLHIFHDCKFAKGCWRFFGLNYNAGAIE 467
RN +P R L R V+T + CP+C S++E LH +C A+ W GL G
Sbjct: 527 AARNCLPTRFALTIRHVDTPMCCPICRSELETTLHALVECVAARDVWDESGLAMLQGNFG 586
Query: 468 SCPEWVLHCLATESHEKSIQMATVLWGIWVARNMIVWEQKPLTPELAMHWSARQIAQWRE 647
S +W+ A + V WG+W RN +VW + + ++ + W
Sbjct: 587 SFVDWLATMFAYCDFVVFAKYLAVCWGLWWRRNDVVWNGRIWHSQQVVNGCFTMLESWFH 646
Query: 648 VQALKGSSIKGRTQGQQQSVVRWKAPEEGQFKINVDAAVQLGRPD-FKVGMVLRDHRGNF 824
+++ + +W+ P+ G KINVD AV PD +G V RDH+G F
Sbjct: 647 ANETLATAVTVPSYSS-----KWQKPDYGWIKINVDGAV---FPDKGAIGAVFRDHQGRF 698
Query: 825 CNARVACLGGEVTVFEAEARGMLEAVRWATELGISNVIFESDSMLTVQALNRKTINYLEV 1004
+ EA G+ E + W E S ++ E+D + VQA+ K+
Sbjct: 699 MGGFAKPFPHQTLPKVVEALGVREVLSWIHERSRSRIVVETDCLRVVQAIQHKSCPNTSF 758
Query: 1005 GNMLQDCRSLINDRPDFSVTFVRRQANKVAHLLARVPCDAYCFH 1136
G ++ DC ++ D V + RR AN AH LA C H
Sbjct: 759 GFIIVDCLDVLQHLVDVQVVYARRSANSAAHCLANGACSFTSLH 802
>gb|ABD28710.1| Polynucleotidyl transferase, Ribonuclease H fold [Medicago
truncatula]
Length = 393
Score = 158 bits (399), Expect = 3e-36
Identities = 100/360 (27%), Positives = 161/360 (44%), Gaps = 18/360 (5%)
Frame = +3
Query: 117 SDRIAWMHTIDGKYTVKSGYSFWQKHFGQCNQGATHKGWTKLWNLEVPQKVKVFLWRICR 296
+D + W +G Y+VKS Y + W+ +W L+VP +VK +WR+CR
Sbjct: 18 TDSLLWKVEKNGCYSVKSAYRVCVEDVVNNAHLRKPGYWSGIWRLKVPPRVKKLVWRVCR 77
Query: 297 NNIPVRNLLRGRGVNTTILCPMCGSDVEHLLHIFHDCKFAKGCWRFFGLNY----NAGAI 464
P R L RGVN C C E HIF C+ A W + + +
Sbjct: 78 ECFPTRVRLISRGVNCPSACVKCEDPHEDCYHIFFHCRTAIDVWNTANVWHLIAPSLSQF 137
Query: 465 ESCPEWVLHCLATESHEKSIQMATVLWGIWVARNMIVWEQKPLTPELAMHWSARQIAQWR 644
++ P+ + + L S + + T++W IW +RN+ +W+Q + + + + WR
Sbjct: 138 DNAPDIIFNLLQKLSASQMESIVTIMWSIWKSRNLKLWQQVSESSVTILERAKHLLEGWR 197
Query: 645 EVQALKG--------SSIKGRTQGQQQSV-----VRWKAPEEGQFKINVDAAVQLGRPDF 785
+ +G ++ + +T Q + +RW+ P+ G+ K NVDA+
Sbjct: 198 KANHKQGLLGQVHSPTNSRPQTHDSQNTDNRYGNIRWRKPKSGRLKCNVDASFSTSSNKV 257
Query: 786 KVGMVLRDHRGNFCNARVACLGGEVTVFEAEARGMLEAVRWATELGISNVIFESDSMLTV 965
+GM +RD GN ++ V EA G+ A RW EL ++NV FE DS
Sbjct: 258 GIGMCIRDSEGNHVRSKTMWFSPLCPVNIGEALGLYHATRWINELQLTNVDFEVDSKTIA 317
Query: 966 QALNRKTINYLEVGNMLQDCRSLIN-DRPDFSVTFVRRQANKVAHLLARVPCDAYCFHDF 1142
N+ + E G+++++ N + V F RRQAN+VAH LA+ FH F
Sbjct: 318 DYFNKARGDNTEFGSIMENTIQFCNIFLTNSHVEFTRRQANEVAHELAKAATLGPSFHIF 377
>gb|ABN06084.1| Polynucleotidyl transferase, Ribonuclease H fold [Medicago
truncatula]
Length = 519
Score = 152 bits (383), Expect = 2e-34
Identities = 99/362 (27%), Positives = 159/362 (43%), Gaps = 33/362 (9%)
Frame = +3
Query: 120 DRIAWMHTIDGKYTVKSGYSFWQKHFGQCNQGATHKGWTKLWNLEVPQKVKVFLWRICRN 299
DRI W DGKY+V+S Y+ + + W+ +W L+VP K+K F+WR+CR
Sbjct: 131 DRIIWKAENDGKYSVRSAYTLCVEELIDTSFLRRPGYWSDIWRLKVPPKIKNFMWRVCRG 190
Query: 300 NIPVRNLLRGRGVNTTILCPMCGSDVEHLLHIFHDCKFAKGCWRFFGL----NYNAGAIE 467
+P RN LR +GV C C + E + H+F C F W+ L +
Sbjct: 191 VLPTRNKLRDKGVQCPEACVNCSTTSEDVKHLFCVCTFDVQVWQLSDLWSEIQQAVIHFD 250
Query: 468 SCPEWVLHCLATESHEKSIQMATVLWGIWVARNMIVWEQKPLTPELAMHWSARQIAQWRE 647
+ + L + E+S Q+ +W +W RN+ VWE K + + + + + +W+
Sbjct: 251 TASAVIFDLLQRFTLEQS-QIFVTMWSLWKRRNLKVWENKNESCAMTVDRARVMMEEWQL 309
Query: 648 VQA--------------------------LKGSSIKGRTQGQQQSV--VRWKAPEEGQFK 743
A L+ S + QQ V + W+ P G+FK
Sbjct: 310 ATATRSAPTHQHTHLQNPSAATAQQPVVHLQQSHVASAVSAQQSLVPSISWERPSRGRFK 369
Query: 744 INVDAAVQLGRPDFKVGMVLRDHRGNFCNARVACLGGEVTVFEAEARGMLEAVRWATELG 923
NVDA + +G+ +RD G + A+ V EA G+ A+ W +++
Sbjct: 370 CNVDAGFSTSKNRIGIGICVRDDDGAYVLAKTISFDVVHPVRVGEALGLYHALEWLSDMQ 429
Query: 924 ISNVIFESDSMLTVQALNRKTINYLEVGNMLQDCRSLINDR-PDFSVTFVRRQANKVAHL 1100
N+ F DS +T A N + + E G+++ +SL + + V F+RRQAN AH
Sbjct: 430 FDNMDFAMDSKITYDAFNSQKDDISEFGHIISARQSLFSTHFTNSRVEFIRRQANAAAHA 489
Query: 1101 LA 1106
A
Sbjct: 490 FA 491
>gb|ABD32189.2| Polynucleotidyl transferase, Ribonuclease H fold [Medicago
truncatula]
Length = 612
Score = 152 bits (383), Expect = 2e-34
Identities = 99/362 (27%), Positives = 159/362 (43%), Gaps = 33/362 (9%)
Frame = +3
Query: 120 DRIAWMHTIDGKYTVKSGYSFWQKHFGQCNQGATHKGWTKLWNLEVPQKVKVFLWRICRN 299
DRI W DGKY+V+S Y+ + + W+ +W L+VP K+K F+WR+CR
Sbjct: 224 DRIIWKAENDGKYSVRSAYTLCVEELIDTSFLRRPGYWSDIWRLKVPPKIKNFMWRVCRG 283
Query: 300 NIPVRNLLRGRGVNTTILCPMCGSDVEHLLHIFHDCKFAKGCWRFFGL----NYNAGAIE 467
+P RN LR +GV C C + E + H+F C F W+ L +
Sbjct: 284 VLPTRNKLRDKGVQCPEACVNCSTTSEDVKHLFCVCTFDVQVWQLSDLWSEIQQAVIHFD 343
Query: 468 SCPEWVLHCLATESHEKSIQMATVLWGIWVARNMIVWEQKPLTPELAMHWSARQIAQWRE 647
+ + L + E+S Q+ +W +W RN+ VWE K + + + + + +W+
Sbjct: 344 TASAVIFDLLQRFTLEQS-QIFVTMWSLWKRRNLKVWENKNESCAMTVDRARVMMEEWQL 402
Query: 648 VQA--------------------------LKGSSIKGRTQGQQQSV--VRWKAPEEGQFK 743
A L+ S + QQ V + W+ P G+FK
Sbjct: 403 ATATRSAPTHQHTHLQNPSAATAQQPVVHLQQSHVASAVSAQQSLVPSISWERPSRGRFK 462
Query: 744 INVDAAVQLGRPDFKVGMVLRDHRGNFCNARVACLGGEVTVFEAEARGMLEAVRWATELG 923
NVDA + +G+ +RD G + A+ V EA G+ A+ W +++
Sbjct: 463 CNVDAGFSTSKNRIGIGICVRDDDGAYVLAKTISFDVVHPVRVGEALGLYHALEWLSDMQ 522
Query: 924 ISNVIFESDSMLTVQALNRKTINYLEVGNMLQDCRSLINDR-PDFSVTFVRRQANKVAHL 1100
N+ F DS +T A N + + E G+++ +SL + + V F+RRQAN AH
Sbjct: 523 FDNMDFAMDSKITYDAFNSQKDDISEFGHIISARQSLFSTHFTNSRVEFIRRQANAAAHA 582
Query: 1101 LA 1106
A
Sbjct: 583 FA 584