BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00031209
         (1124 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002520211.1| Metalloendoproteinase 1 precursor, putative ...   410   e-112
emb|CCH68442.1| matrix metalloproteinase precursor [Solanum lyco...   410   e-112
gb|ABF58910.1| matrix metalloprotease 1 [Nicotiana tabacum]           407   e-111
gb|ADD21635.1| matrix metalloprotease 1 [Nicotiana benthamiana]       403   e-110
ref|XP_002267298.1| PREDICTED: metalloendoproteinase 1-like [Vit...   402   e-109

>ref|XP_002520211.1| Metalloendoproteinase 1 precursor, putative [Ricinus communis]
            gi|223540703|gb|EEF42266.1| Metalloendoproteinase 1
            precursor, putative [Ricinus communis]
          Length = 364

 Score =  410 bits (1054), Expect = e-112
 Identities = 205/338 (60%), Positives = 252/338 (74%), Gaps = 10/338 (2%)
 Frame = +2

Query: 62   LNAFPSDFIPRFFPNASSLPQ---ANLTF-AWQSFNNLTGCRSGESREGLAKLKQYFQQF 229
            L +FPS    RFFPN SS+P     N+T   W SF  L GCR+G+  EGL+KLK YF  F
Sbjct: 14   LVSFPSTSA-RFFPNVSSIPPNLLPNITSPTWDSFKRLAGCRAGDKLEGLSKLKNYFHYF 72

Query: 230  GYL-NNYQNFTDDFDNSLESALKTYQRNFNLNSTGELDQTTLNQILSPRCGVADIVNGTS 406
            GY+ N   NFTDDFD++LESA+KTYQ+NFNLN TGELD+ TL+Q++ PRCG ADI+NG++
Sbjct: 73   GYIPNTLSNFTDDFDDALESAIKTYQQNFNLNVTGELDEQTLDQLVRPRCGNADIINGST 132

Query: 407  TMNSGK--SPSSPLHVVAHYSYFPGSPRWPTGQDQLTYSFAPENPLDDNVKSIFRAAFDR 580
            TMNSGK  + ++  H V HYS+FPG+PRWP  +  LTY+F P N L +  KS+F  AFDR
Sbjct: 133  TMNSGKWQTTTTSFHAVGHYSFFPGTPRWPDSKRDLTYAFWPSNQLTEVEKSVFTRAFDR 192

Query: 581  WSEVTTLTFLQTEAYRAADIKIGFYRGDHGDGEAFDGVLGTLAHAFSPPSGRLHLDGDEN 760
            WS V  + F +TE+Y  ADI+IGF+ GDHGDGE FDGVLGTLAHAFSPPSGR HLDG+EN
Sbjct: 193  WSTVIPMNFTETESYNVADIRIGFFSGDHGDGEPFDGVLGTLAHAFSPPSGRFHLDGEEN 252

Query: 761  WVINGDDLKST--SAVDLESVVVHEIGHLLGLGHSSVEDAVMFPSIPSGAKKIQLAGDDV 934
            WV++GD   ST  SAVDLESV VHEIGHLLGLGHSSVE+A+M+P+IPS  +KI+LA DD+
Sbjct: 253  WVVSGDISSSTISSAVDLESVAVHEIGHLLGLGHSSVEEAIMYPTIPSRTRKIELANDDI 312

Query: 935  AGIQSLYGSNPAY-VAGSNPAGTQERETNDGRFVGPVW 1045
             GIQ LYGSNP +  + S+    QERET+       +W
Sbjct: 313  QGIQYLYGSNPNFNGSSSSTPSIQERETSGAYHARSLW 350


>emb|CCH68442.1| matrix metalloproteinase precursor [Solanum lycopersicum]
          Length = 367

 Score =  410 bits (1053), Expect = e-112
 Identities = 206/354 (58%), Positives = 254/354 (71%), Gaps = 15/354 (4%)
 Frame = +2

Query: 35   LFITLSSSFLNAFPSDFIPRFFPNASSLP----QANLTFAWQSFNNLTGCRSGESREGLA 202
            LFI +       FPS     FFPN SS+P    + N T AW +FN L GC SG++ +GLA
Sbjct: 5    LFIAILFVLSVPFPSS--AHFFPNISSIPPNLLKPNAT-AWDAFNKLLGCHSGQTVDGLA 61

Query: 203  KLKQYFQQFGYLNNYQ-NFTDDFDNSLESALKTYQRNFNLNSTGELDQTTLNQILSPRCG 379
            K+K+YF  FGY+NN   NFTDDFD++LESALKTYQ NFNLN+TG LD  T+  ++ PRCG
Sbjct: 62   KIKKYFHYFGYINNSSTNFTDDFDDTLESALKTYQLNFNLNTTGVLDANTIQHLIKPRCG 121

Query: 380  VADIVNGTSTMNSGKSP--SSPLHVVAHYSYFPGSPRWPTGQDQLTYSFAPENPLDDNVK 553
             AD+VNGTSTMNSGK P  S  +H VAHYS+FPGSPRWP  +  LTY+FAP+N L D++K
Sbjct: 122  NADVVNGTSTMNSGKPPAGSPTMHTVAHYSFFPGSPRWPANKRDLTYAFAPQNGLTDDIK 181

Query: 554  SIFRAAFDRWSEVTTLTFLQTEAYRAADIKIGFYRGDHGDGEAFDGVLGTLAHAFSPPSG 733
             +F  AFDRWSEVT LTF +  +Y++ADIKIGF+ GDH DGE FDG +GTLAHAFSPP+G
Sbjct: 182  IVFTRAFDRWSEVTPLTFTEIASYQSADIKIGFFSGDHNDGEPFDGPMGTLAHAFSPPAG 241

Query: 734  RLHLDGDENWVINGDDL------KSTSAVDLESVVVHEIGHLLGLGHSSVEDAVMFPSIP 895
              HLDG+ENWVI+G  +         SAVDLESV VHEIGHLLGLGHSSVEDA+M+P++ 
Sbjct: 242  HFHLDGEENWVIDGAPIVDGNFFSILSAVDLESVAVHEIGHLLGLGHSSVEDAIMYPTLG 301

Query: 896  SGAKKIQLAGDDVAGIQSLYGSNPAYVAGSNP--AGTQERETNDGRFVGPVWGH 1051
            +G ++++L  DD+ G+Q LYGSNP Y  G NP    +QE +TN        W H
Sbjct: 302  AGTRRVELRNDDILGVQELYGSNPNY-TGPNPNLTPSQESDTNGAPIFELSWFH 354


>gb|ABF58910.1| matrix metalloprotease 1 [Nicotiana tabacum]
          Length = 365

 Score =  407 bits (1045), Expect = e-111
 Identities = 195/335 (58%), Positives = 248/335 (74%), Gaps = 14/335 (4%)
 Frame = +2

Query: 95   FFPNASSLPQANL---TFAWQSFNNLTGCRSGESREGLAKLKQYFQQFGYLNNYQNFTDD 265
            F PN SS+P + L     AW +F+ L GC +G+  +GLAK+K+YF  FGY+ +  NFTDD
Sbjct: 22   FSPNISSIPPSLLKPNNTAWDAFHKLLGCHAGQKVDGLAKIKKYFYNFGYIPSLSNFTDD 81

Query: 266  FDNSLESALKTYQRNFNLNSTGELDQTTLNQILSPRCGVADIVNGTSTMNSGKSP--SSP 439
            FD++LESALKTYQ+NFNLN+TG LD  T+  ++ PRCG AD+VNGTSTMNSGK P  S  
Sbjct: 82   FDDALESALKTYQQNFNLNTTGVLDAPTIQHLIRPRCGNADVVNGTSTMNSGKPPAGSQN 141

Query: 440  LHVVAHYSYFPGSPRWPTGQDQLTYSFAPENPLDDNVKSIFRAAFDRWSEVTTLTFLQTE 619
            +H VAH+S+FPG PRWP  +  LTY+F P+N L DN+KS+F  AFDRWSEVT L+F +T 
Sbjct: 142  MHTVAHFSFFPGRPRWPDSKTDLTYAFLPQNGLTDNIKSVFSRAFDRWSEVTPLSFTETA 201

Query: 620  AYRAADIKIGFYRGDHGDGEAFDGVLGTLAHAFSPPSGRLHLDGDENWVIN------GDD 781
            ++++ADIKIGF+ GDH DGE FDG +GTLAHAFSPP G  HLDGDENWVI+      G+ 
Sbjct: 202  SFQSADIKIGFFAGDHNDGEPFDGPMGTLAHAFSPPGGHFHLDGDENWVIDGVPIVEGNF 261

Query: 782  LKSTSAVDLESVVVHEIGHLLGLGHSSVEDAVMFPSIPSGAKKIQLAGDDVAGIQSLYGS 961
                SAVDLESV VHEIGHLLGLGHSSVED++MFPS+ +G ++++LA DD+ G+Q LYGS
Sbjct: 262  FSILSAVDLESVAVHEIGHLLGLGHSSVEDSIMFPSLAAGTRRVELANDDIQGVQVLYGS 321

Query: 962  NPAYVAGS---NPAGTQERETNDGRFVGPVWGHMI 1057
            NP +   +   NP  TQE +TN     G +W H++
Sbjct: 322  NPNFTGPNTVLNP--TQENDTNGAPKFGSLWVHVV 354


>gb|ADD21635.1| matrix metalloprotease 1 [Nicotiana benthamiana]
          Length = 364

 Score =  403 bits (1036), Expect = e-110
 Identities = 195/334 (58%), Positives = 247/334 (73%), Gaps = 13/334 (3%)
 Frame = +2

Query: 95   FFPNASSLP---QANLTFAWQSFNNLTGCRSGESREGLAKLKQYFQQFGYLNNYQNFTDD 265
            FFPN SS+P   + N T AW +F+ L GC +G+  +GLAK+K+YF  FGY+++  NFTDD
Sbjct: 22   FFPNISSIPPLLKPNNT-AWDAFHKLLGCHAGQKVDGLAKIKKYFYNFGYISSLSNFTDD 80

Query: 266  FDNSLESALKTYQRNFNLNSTGELDQTTLNQILSPRCGVADIVNGTSTMNSGK--SPSSP 439
            FD++LESALKTYQ+NFNLN+TG LD  T+  ++ PRCG AD+VNGTSTMNSGK  + S  
Sbjct: 81   FDDALESALKTYQQNFNLNTTGVLDAPTIEHLIRPRCGNADVVNGTSTMNSGKPSAGSQN 140

Query: 440  LHVVAHYSYFPGSPRWPTGQDQLTYSFAPENPLDDNVKSIFRAAFDRWSEVTTLTFLQTE 619
            +H VAH+S+FPG PRWP     LTY+F P+N L DN+KS+F  AFDRWSEVT LTF +  
Sbjct: 141  IHTVAHFSFFPGRPRWPESNRDLTYAFLPQNGLTDNIKSVFSRAFDRWSEVTPLTFTEIA 200

Query: 620  AYRAADIKIGFYRGDHGDGEAFDGVLGTLAHAFSPPSGRLHLDGDENWVINGDDL----- 784
            ++++ADIKIGF+ GDH DGE FDG +GTLAHAFSPP G  HLDGDENWVI+G  +     
Sbjct: 201  SFQSADIKIGFFAGDHNDGEPFDGPMGTLAHAFSPPGGHFHLDGDENWVIDGAPIVEGNF 260

Query: 785  -KSTSAVDLESVVVHEIGHLLGLGHSSVEDAVMFPSIPSGAKKIQLAGDDVAGIQSLYGS 961
                SAVDLESV VHEIGHLLGLGHSSVED++MFPS+ +G ++++LA DD+ G+Q LYGS
Sbjct: 261  FSILSAVDLESVAVHEIGHLLGLGHSSVEDSIMFPSLAAGTRRVELANDDIQGVQVLYGS 320

Query: 962  NPAYVAGSNP--AGTQERETNDGRFVGPVWGHMI 1057
            NP +  G N     T E +TN     G +W H++
Sbjct: 321  NPNF-TGPNTVLTPTHENDTNGALKFGSLWVHVV 353


>ref|XP_002267298.1| PREDICTED: metalloendoproteinase 1-like [Vitis vinifera]
          Length = 364

 Score =  402 bits (1032), Expect = e-109
 Identities = 201/358 (56%), Positives = 259/358 (72%), Gaps = 12/358 (3%)
 Frame = +2

Query: 8    MSLNLITALLFITLSSSFLNAFPSDFIPRFFPNASSLPQAN----LTFAWQSFNNLTGCR 175
            M  +++ A +F+ L ++ ++A       RFFPNA S+P ++     T  W +F    GC 
Sbjct: 1    MRFHVLCAAIFLPLLTTSVSA-------RFFPNAPSMPPSSPPNATTSGWDAFKKFAGCH 53

Query: 176  SGESREGLAKLKQYFQQFGYLNNYQNFTDDFDNSLESALKTYQRNFNLNSTGELDQTTLN 355
            +G+  +GLAKLK+YF  FGY++N  N+TDDFD++ E ALKTYQ NFNLN+TG+LD+ TLN
Sbjct: 54   AGDKADGLAKLKEYFHYFGYIHN-SNYTDDFDDAFEQALKTYQLNFNLNTTGQLDEATLN 112

Query: 356  QILSPRCGVADIVNGTSTMNSGKSPSSP---LHVVAHYSYFPGSPRWPTGQDQLTYSFAP 526
            QI+SPRCG ADI NG+S+MNSGKS  S     H V HYS+F G P WP  +  LTY F P
Sbjct: 113  QIVSPRCGNADIENGSSSMNSGKSTPSTSGHFHTVGHYSFFDGKPVWPESKRNLTYGFLP 172

Query: 527  ENPLDDNVKSIFRAAFDRWSEVTTLTFLQTEAYRAADIKIGFYRGDHGDGEAFDGVLGTL 706
            +N L D VK++F +AF+RW+ VT LTF ++++Y +AD++I FY GDHGDGEAFDGVLGTL
Sbjct: 173  DNQLSDTVKAVFTSAFERWAAVTPLTFTESDSYYSADLRIAFYTGDHGDGEAFDGVLGTL 232

Query: 707  AHAFSPPSGRLHLDGDENWVINGDDLKS--TSAVDLESVVVHEIGHLLGLGHSSVEDAVM 880
            AHAFSP +GR HLDG+ENWV  GD   S  +SA+DLESV VHEIGHLLGLGHSSVE+A+M
Sbjct: 233  AHAFSPTNGRFHLDGEENWVATGDVTTSSISSAIDLESVAVHEIGHLLGLGHSSVEEAIM 292

Query: 881  FPSIPSGAKKIQLAGDDVAGIQSLYGSNPAYVAGSNPAGTQERET--NDGRF-VGPVW 1045
            +P+I S  KK++LA DD+ GIQ LYGSNP Y  GS+ + +QE ET  N G +   P W
Sbjct: 293  YPTITSRTKKVELASDDIQGIQELYGSNPNY-NGSSTSPSQESETTNNSGAYTAAPRW 349