BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00031023
         (740 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAC62932.1| antifreeze protein [Daucus carota] gi|4455920|emb...   347   1e-93
gb|AFW20019.1| antifreeze protein [Daucus carota]                     347   2e-93
gb|AAV66074.1| antifreeze protein [Daucus carota]                     333   2e-89
gb|AFQ39766.1| polygalacturonase-inhibiting protein [Vitis rupes...   239   6e-61
gb|ABU82741.1| polygalacturonase-inhibiting protein [Vitis thunb...   236   3e-60

>gb|AAC62932.1| antifreeze protein [Daucus carota] gi|4455920|emb|CAB37347.1|
           antifreeze polypeptide [Daucus carota]
          Length = 332

 Score =  347 bits (891), Expect = 1e-93
 Identities = 168/201 (83%), Positives = 182/201 (90%)
 Frame = -3

Query: 738 NLAGPVPLFFPQLKKLVCLDLSFNKLLGVIPPQLSTLPTLCALHLDHNELTGEIPDIFGN 559
           +L+GPVPLFFPQL KL CLDLSFNKLLGVIPPQLSTLP L ALHL+ NELTGEIPDIFGN
Sbjct: 133 SLSGPVPLFFPQLTKLTCLDLSFNKLLGVIPPQLSTLPNLKALHLERNELTGEIPDIFGN 192

Query: 558 FAGSPDIYLSHNQLTGFVPKTFASSDPINIDFSWNRLEGDISFFFGHNKRMEMADFSGNV 379
           FAGSPDIYLSHNQLTGFVPKTFA +DPI +DFS NRLEGDISF FG  KR+EM DFSGNV
Sbjct: 193 FAGSPDIYLSHNQLTGFVPKTFARADPIRLDFSGNRLEGDISFLFGPKKRLEMLDFSGNV 252

Query: 378 LSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVMKLQTFNVSNNNLCGKIPTGGNLQ 199
           LSF+F++VQEFPPSLTYLDLNHNQI+G L S LAK + LQTFNVS+NNLCGKIPTGGNLQ
Sbjct: 253 LSFNFSRVQEFPPSLTYLDLNHNQISGSLSSELAK-LDLQTFNVSDNNLCGKIPTGGNLQ 311

Query: 198 RFDSTSYLHNSCLCGSPLEEC 136
           RFD T+YLHNSCLCG+PL EC
Sbjct: 312 RFDRTAYLHNSCLCGAPLPEC 332


>gb|AFW20019.1| antifreeze protein [Daucus carota]
          Length = 332

 Score =  347 bits (889), Expect = 2e-93
 Identities = 168/201 (83%), Positives = 182/201 (90%)
 Frame = -3

Query: 738 NLAGPVPLFFPQLKKLVCLDLSFNKLLGVIPPQLSTLPTLCALHLDHNELTGEIPDIFGN 559
           +L+GPVPLFFPQL KL CLDLSFNKLLGVIPPQLSTLP L ALHL+ N+LTGEIPDIFGN
Sbjct: 133 SLSGPVPLFFPQLTKLTCLDLSFNKLLGVIPPQLSTLPNLKALHLEQNQLTGEIPDIFGN 192

Query: 558 FAGSPDIYLSHNQLTGFVPKTFASSDPINIDFSWNRLEGDISFFFGHNKRMEMADFSGNV 379
           FAGSPDIYLSHNQLTGFVPKTFASSDPI +DFS NRLEGDISF FG NKR+E  DFSGNV
Sbjct: 193 FAGSPDIYLSHNQLTGFVPKTFASSDPIRLDFSGNRLEGDISFLFGPNKRLETLDFSGNV 252

Query: 378 LSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVMKLQTFNVSNNNLCGKIPTGGNLQ 199
           LSF+F++VQ FPPSLTYLDLNHNQI+G L S LAK + LQTFNVS+NNLCGKIPTGGNLQ
Sbjct: 253 LSFNFSRVQGFPPSLTYLDLNHNQISGSLSSELAK-LDLQTFNVSDNNLCGKIPTGGNLQ 311

Query: 198 RFDSTSYLHNSCLCGSPLEEC 136
           RFD T+YLHNSCLCG+PL EC
Sbjct: 312 RFDRTAYLHNSCLCGAPLPEC 332


>gb|AAV66074.1| antifreeze protein [Daucus carota]
          Length = 332

 Score =  333 bits (854), Expect = 2e-89
 Identities = 162/201 (80%), Positives = 177/201 (88%)
 Frame = -3

Query: 738 NLAGPVPLFFPQLKKLVCLDLSFNKLLGVIPPQLSTLPTLCALHLDHNELTGEIPDIFGN 559
           +L+GPVPLFFPQL KL CLDLSFNKLLGVIPPQLSTL  L ALHL+ NELTGEIPDIFGN
Sbjct: 133 SLSGPVPLFFPQLTKLTCLDLSFNKLLGVIPPQLSTLANLKALHLERNELTGEIPDIFGN 192

Query: 558 FAGSPDIYLSHNQLTGFVPKTFASSDPINIDFSWNRLEGDISFFFGHNKRMEMADFSGNV 379
           FAGSPDIYLSHNQLTGFVPKTF  +DPI +DFS NRLEGDISF FG  KR+E  DFSGN 
Sbjct: 193 FAGSPDIYLSHNQLTGFVPKTFGRADPIRLDFSGNRLEGDISFLFGPKKRLETLDFSGNE 252

Query: 378 LSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVMKLQTFNVSNNNLCGKIPTGGNLQ 199
           LSF+F++VQEFPPSLTYLDLNHNQI+G L S LAK + LQTFNVS+NNLCGKIPTGGNLQ
Sbjct: 253 LSFNFSRVQEFPPSLTYLDLNHNQISGSLSSELAK-LDLQTFNVSDNNLCGKIPTGGNLQ 311

Query: 198 RFDSTSYLHNSCLCGSPLEEC 136
           RFD T+YL +SCLCG+PL EC
Sbjct: 312 RFDRTAYLRDSCLCGAPLPEC 332


>gb|AFQ39766.1| polygalacturonase-inhibiting protein [Vitis rupestris x Vitis
           vinifera]
          Length = 333

 Score =  239 bits (609), Expect = 6e-61
 Identities = 121/202 (59%), Positives = 142/202 (70%), Gaps = 1/202 (0%)
 Frame = -3

Query: 738 NLAGPVPLFFPQLKKLVCLDLSFNKLLGVIPPQLSTLPTLCALHLDHNELTGEIPDIFGN 559
           NL+GPVP FF +LK L  LDLSFN L G IP  LS LP L ALHLD N+LTG IPD FG 
Sbjct: 133 NLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNDLTGPIPDSFGK 192

Query: 558 FAGS-PDIYLSHNQLTGFVPKTFASSDPINIDFSWNRLEGDISFFFGHNKRMEMADFSGN 382
           FAGS PD+YLSHNQL+G +P +F   DP  +D S N+LEGD+S FF  NK  ++ DFS N
Sbjct: 193 FAGSTPDLYLSHNQLSGKIPYSFRGFDPTVMDLSRNKLEGDLSIFFNANKSTQIVDFSRN 252

Query: 381 VLSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVMKLQTFNVSNNNLCGKIPTGGNL 202
           +  FD ++V EFP SLT LDL+HN+I G LP  +   + LQ  NVS N LCGKIP GG L
Sbjct: 253 LFQFDLSRV-EFPQSLTSLDLSHNKIAGSLPEMMTS-LDLQFLNVSYNRLCGKIPVGGKL 310

Query: 201 QRFDSTSYLHNSCLCGSPLEEC 136
           Q FD  SY HN CLCG+PL+ C
Sbjct: 311 QSFDYDSYFHNRCLCGAPLQSC 332



 Score = 58.5 bits (140), Expect = 1e-06
 Identities = 44/147 (29%), Positives = 66/147 (44%), Gaps = 3/147 (2%)
 Frame = -3

Query: 615 ALHLDHNELTGEIPDIFGNFAGSPD-IYLSHNQLTGFVPKTFASSDPIN-IDFSWNRLEG 442
           +L +   +L+G+IPD  G+       I+   + LTG +P   A    +  +  SW  L G
Sbjct: 77  SLTIFSGQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSG 136

Query: 441 DISFFFGHNKRMEMADFSGNVLSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVM-K 265
            +  FF   K +   D S N LS          P+L  L L+ N +TG +P +  K    
Sbjct: 137 PVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNDLTGPIPDSFGKFAGS 196

Query: 264 LQTFNVSNNNLCGKIPTGGNLQRFDST 184
                +S+N L GKIP   + + FD T
Sbjct: 197 TPDLYLSHNQLSGKIPY--SFRGFDPT 221


>gb|ABU82741.1| polygalacturonase-inhibiting protein [Vitis thunbergii]
          Length = 333

 Score =  236 bits (603), Expect = 3e-60
 Identities = 121/202 (59%), Positives = 140/202 (69%), Gaps = 1/202 (0%)
 Frame = -3

Query: 738 NLAGPVPLFFPQLKKLVCLDLSFNKLLGVIPPQLSTLPTLCALHLDHNELTGEIPDIFGN 559
           NL+GPVP FF +LK L  LDLSFN L G IP  LS LP L ALHLD N LTG IPD FG 
Sbjct: 133 NLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLDALHLDRNHLTGPIPDSFGK 192

Query: 558 FAGS-PDIYLSHNQLTGFVPKTFASSDPINIDFSWNRLEGDISFFFGHNKRMEMADFSGN 382
           FAGS P +YLSHNQL+G +P +F   DPI +D S N+LEGD+S FF  NK  +  DFS N
Sbjct: 193 FAGSTPGLYLSHNQLSGKIPYSFREFDPIVMDLSRNKLEGDLSIFFNANKSTQTVDFSRN 252

Query: 381 VLSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVMKLQTFNVSNNNLCGKIPTGGNL 202
           +  FD ++V EFP SLT LDL+HN+I G LP  +   + LQ  NVS N LCGKIP GG L
Sbjct: 253 LFQFDLSRV-EFPKSLTSLDLSHNKIAGSLPEMMTS-LDLQFLNVSYNRLCGKIPVGGKL 310

Query: 201 QRFDSTSYLHNSCLCGSPLEEC 136
           Q FD  SY HN CLCG+PL+ C
Sbjct: 311 QSFDYDSYFHNRCLCGAPLQSC 332



 Score = 58.2 bits (139), Expect = 2e-06
 Identities = 41/136 (30%), Positives = 61/136 (44%), Gaps = 3/136 (2%)
 Frame = -3

Query: 615 ALHLDHNELTGEIPDIFGNFAGSPD-IYLSHNQLTGFVPKTFASSDPIN-IDFSWNRLEG 442
           +L +   +L+G+IPD  G+       I+   + LTG +P   A    +  +  SW  L G
Sbjct: 77  SLTIFSGQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSG 136

Query: 441 DISFFFGHNKRMEMADFSGNVLSFDFTKVQEFPPSLTYLDLNHNQITGGLPSALAKVM-K 265
            +  FF   K +   D S N LS          P+L  L L+ N +TG +P +  K    
Sbjct: 137 PVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLDALHLDRNHLTGPIPDSFGKFAGS 196

Query: 264 LQTFNVSNNNLCGKIP 217
                +S+N L GKIP
Sbjct: 197 TPGLYLSHNQLSGKIP 212