BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00030801
         (464 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279813.1| PREDICTED: probable polygalacturonase non-ca...   106   5e-36
emb|CBI15046.3| unnamed protein product [Vitis vinifera]              106   5e-36
ref|XP_002318036.1| predicted protein [Populus trichocarpa] gi|2...    99   6e-33
emb|CCD74525.1| polygalacturonase 1 [Arabidopsis halleri subsp. ...    89   7e-33
ref|NP_176242.1| polygalacturonase 1 [Arabidopsis thaliana] gi|7...    89   7e-33

>ref|XP_002279813.1| PREDICTED: probable polygalacturonase non-catalytic subunit
           JP650-like [Vitis vinifera]
          Length = 630

 Score =  106 bits (264), Expect(2) = 5e-36
 Identities = 53/89 (59%), Positives = 65/89 (73%), Gaps = 2/89 (2%)
 Frame = -2

Query: 454 NPFTAKASLIRYWNKQISNNLTKPEFLFSKASPLSAVNTAYFSKLAASKKLDSQLSSFCS 275
           NPFTAKASLIRYWNKQISN L KP FL SKASPL+AV++A  +KLA    L S LSSFCS
Sbjct: 37  NPFTAKASLIRYWNKQISNKLPKPSFLLSKASPLNAVDSAVLTKLATQNALSSHLSSFCS 96

Query: 274 AAKLFCLHTKHTPNDDD--NTHFSFYSNK 194
           +A LFC+      ++    + +F+FYSN+
Sbjct: 97  SANLFCVFDSSPTSEKQVKDANFAFYSNR 125



 Score = 70.1 bits (170), Expect(2) = 5e-36
 Identities = 31/61 (50%), Positives = 42/61 (68%)
 Frame = -3

Query: 183 YGDSHLSGSDNFKNYSGGNNFATASFGRYSRSSTNHNVGFSTYAKDANVASSNFTSYAAS 4
           YGDS + G D+FKNYS G N  + SF RYS  ST+H+  F++YA+D NVA+ +F  Y + 
Sbjct: 130 YGDSRIGGVDSFKNYSDGLNMPSGSFRRYSGDSTDHHEEFTSYARDGNVATGSFAGYGSG 189

Query: 3   A 1
           A
Sbjct: 190 A 190


>emb|CBI15046.3| unnamed protein product [Vitis vinifera]
          Length = 602

 Score =  106 bits (264), Expect(2) = 5e-36
 Identities = 53/89 (59%), Positives = 65/89 (73%), Gaps = 2/89 (2%)
 Frame = -2

Query: 454 NPFTAKASLIRYWNKQISNNLTKPEFLFSKASPLSAVNTAYFSKLAASKKLDSQLSSFCS 275
           NPFTAKASLIRYWNKQISN L KP FL SKASPL+AV++A  +KLA    L S LSSFCS
Sbjct: 40  NPFTAKASLIRYWNKQISNKLPKPSFLLSKASPLNAVDSAVLTKLATQNALSSHLSSFCS 99

Query: 274 AAKLFCLHTKHTPNDDD--NTHFSFYSNK 194
           +A LFC+      ++    + +F+FYSN+
Sbjct: 100 SANLFCVFDSSPTSEKQVKDANFAFYSNR 128



 Score = 70.1 bits (170), Expect(2) = 5e-36
 Identities = 31/61 (50%), Positives = 42/61 (68%)
 Frame = -3

Query: 183 YGDSHLSGSDNFKNYSGGNNFATASFGRYSRSSTNHNVGFSTYAKDANVASSNFTSYAAS 4
           YGDS + G D+FKNYS G N  + SF RYS  ST+H+  F++YA+D NVA+ +F  Y + 
Sbjct: 133 YGDSRIGGVDSFKNYSDGLNMPSGSFRRYSGDSTDHHEEFTSYARDGNVATGSFAGYGSG 192

Query: 3   A 1
           A
Sbjct: 193 A 193


>ref|XP_002318036.1| predicted protein [Populus trichocarpa] gi|222858709|gb|EEE96256.1|
           predicted protein [Populus trichocarpa]
          Length = 615

 Score = 98.6 bits (244), Expect(2) = 6e-33
 Identities = 51/89 (57%), Positives = 63/89 (70%), Gaps = 2/89 (2%)
 Frame = -2

Query: 454 NPFTAKASLIRYWNKQISNNLTKPEFLFSKASPLSAVNTAYFSKLAASKKLDSQLSSFCS 275
           NPF+ +ASLIRYWNK ISN+L KP FL SKASPLSA+++A  +KLA    L   L SFCS
Sbjct: 23  NPFSPRASLIRYWNKHISNSLPKPLFLLSKASPLSAIDSAILTKLATQNSLSLHLDSFCS 82

Query: 274 AAKLFC-LHTKHT-PNDDDNTHFSFYSNK 194
            A LFC   TK +  N D +++F+ YSNK
Sbjct: 83  LANLFCFFDTKQSLRNHDQDSNFALYSNK 111



 Score = 67.4 bits (163), Expect(2) = 6e-33
 Identities = 32/61 (52%), Positives = 40/61 (65%)
 Frame = -3

Query: 183 YGDSHLSGSDNFKNYSGGNNFATASFGRYSRSSTNHNVGFSTYAKDANVASSNFTSYAAS 4
           YG S LSG D+FKNYS G N    SF RYSR ST H+  F+ Y  D NVA++ F +Y ++
Sbjct: 116 YGGSRLSGVDSFKNYSNGLNTVADSFIRYSRESTGHSETFTNYGNDGNVANATFGNYGSA 175

Query: 3   A 1
           A
Sbjct: 176 A 176


>emb|CCD74525.1| polygalacturonase 1 [Arabidopsis halleri subsp. halleri]
          Length = 626

 Score = 89.4 bits (220), Expect(2) = 7e-33
 Identities = 48/94 (51%), Positives = 63/94 (67%), Gaps = 5/94 (5%)
 Frame = -2

Query: 460 SKNPFTAKASLIRYWNKQISNNLTKPEFLFSKASPLSAVNTAYFSKLAASKKLDSQLSSF 281
           S+NPFT KASLIRYWN  I+ +  KP F  SKASPL+AV++  F+ LA++  L ++ S F
Sbjct: 36  SENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHALHTRHSDF 95

Query: 280 CSAAKLFC---LHTKHTPND--DDNTHFSFYSNK 194
           CSAAKLFC   L   H+ +D   D+  F+ YS K
Sbjct: 96  CSAAKLFCFPELANAHSISDKHGDDVTFAAYSGK 129



 Score = 76.3 bits (186), Expect(2) = 7e-33
 Identities = 34/58 (58%), Positives = 43/58 (74%)
 Frame = -3

Query: 183 YGDSHLSGSDNFKNYSGGNNFATASFGRYSRSSTNHNVGFSTYAKDANVASSNFTSYA 10
           YG   LSG+D+FKNYSGG+N A  SF RYSR+S  H+ GF+ YA + NVA  +FT+YA
Sbjct: 134 YGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYA 191


>ref|NP_176242.1| polygalacturonase 1 [Arabidopsis thaliana]
           gi|75099907|sp|O80760.1|Y639_ARATH RecName:
           Full=Probable polygalacturonase non-catalytic subunit
           At1g60390; AltName: Full=Aromatic-rich glycoprotein
           At1g60390; Flags: Precursor gi|3249081|gb|AAC24065.1|
           Strong similarity to AR0GP2 gene gb|1762634 from
           Lycopersicon esculentum [Arabidopsis thaliana]
           gi|110743341|dbj|BAE99558.1| hypothetical protein
           [Arabidopsis thaliana] gi|332195560|gb|AEE33681.1|
           polygalacturonase 1 [Arabidopsis thaliana]
          Length = 624

 Score = 89.4 bits (220), Expect(2) = 7e-33
 Identities = 46/92 (50%), Positives = 60/92 (65%), Gaps = 3/92 (3%)
 Frame = -2

Query: 460 SKNPFTAKASLIRYWNKQISNNLTKPEFLFSKASPLSAVNTAYFSKLAASKKLDSQLSSF 281
           S+NPFT KASLIRYWN  I+ +  KP F  SKASPL+AV++  F+ LA++  L++  S F
Sbjct: 36  SENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHALNTHHSDF 95

Query: 280 CSAAKLFC---LHTKHTPNDDDNTHFSFYSNK 194
           CSAAKLFC   L         D+ +F+ YS K
Sbjct: 96  CSAAKLFCFPELAAHSLEKHGDDVNFAAYSGK 127



 Score = 76.3 bits (186), Expect(2) = 7e-33
 Identities = 34/58 (58%), Positives = 43/58 (74%)
 Frame = -3

Query: 183 YGDSHLSGSDNFKNYSGGNNFATASFGRYSRSSTNHNVGFSTYAKDANVASSNFTSYA 10
           YG   LSG+D+FKNYSGG+N A  SF RYSR+S  H+ GF+ YA + NVA  +FT+YA
Sbjct: 132 YGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYA 189