BLASTX nr result
ID: Bupleurum21_contig00030762
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00030762
(1326 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AER08630.1| steroid 22-alpha hydroxylase [Populus euphratica] 677 0.0
ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|2... 672 0.0
emb|CBI17531.3| unnamed protein product [Vitis vinifera] 672 0.0
ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-de... 671 0.0
ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis ... 671 0.0
>gb|AER08630.1| steroid 22-alpha hydroxylase [Populus euphratica]
Length = 490
Score = 677 bits (1747), Expect = 0.0
Identities = 337/434 (77%), Positives = 373/434 (85%), Gaps = 2/434 (0%)
Frame = -2
Query: 1325 MEQHISRYGKIYKSNLFGGPTIVSADADLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 1146
MEQHISRYGKIYKSNLFG PTIVSADA LNRFILQ+EG+LFECSYPRSIGGILGKWSMLV
Sbjct: 62 MEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQSEGRLFECSYPRSIGGILGKWSMLV 121
Query: 1145 LVGDMHRDMRNISLNFLSSARLRTHLLKEVESHTLFVLNSWKHDSTFCAQDEAKKFTFNL 966
LVGDMHR+MRNISLNFLS ARLR+HLLKEVE TL VL+SWK + TF AQDEAKKFTFNL
Sbjct: 122 LVGDMHRNMRNISLNFLSHARLRSHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFNL 181
Query: 965 MAKHIMSLEPGKPETEKLKKEYITFMKGVVSAPLNFPGTAYRKALQSRSTILGFIEERMD 786
MAKH+MSL+PGKPETE+LKKEY+TFMKGVVSAPLNFPGT YRKAL+SRSTIL FIE++MD
Sbjct: 182 MAKHMMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTPYRKALKSRSTILKFIEQKMD 241
Query: 785 ERINQMKERNGDCTVENYDDDLLGWVLKHSNFMKDQILDLVLSLLFAGHETSSVSIALAI 606
ERI ++KE D +DDLLGWVLKHSN +QILDL+LSLLFAGHETSSVSIALAI
Sbjct: 242 ERIARLKEGVEDLE----EDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAI 297
Query: 605 YFLQASPDALHQLREEHAEIQQGKKQSGEAELSWDDYKKMEFTHCVISETLRLGNVVRFL 426
YFLQA P A+ QL+EEH EI + KKQSGE E++WDDYKKMEFT CVISETLRLGNVVRFL
Sbjct: 298 YFLQACPGAIQQLKEEHIEISRAKKQSGEMEMNWDDYKKMEFTQCVISETLRLGNVVRFL 357
Query: 425 HRKALKDVSYKGYDIPCGWKVLPVIAAVHLDASTFDNPQHFNPWRWSHQNT--SEKPAPX 252
HRKA++DV YKGYDIPCGWKVLPVI+AVHLD++ FD PQ FNPWRW H N S +
Sbjct: 358 HRKAVRDVRYKGYDIPCGWKVLPVISAVHLDSTVFDQPQQFNPWRWQHNNARGSSTCSSA 417
Query: 251 XXXXXXSNVFMPFGGGPRLCTGSELAKLEMAVFIHHLVLNFNWELAGHSDHPFAFPFVDF 72
SN FMPFGGGPRLC GSELAKLEMAVFIHHLVLNF+WEL G +D FAFPFVDF
Sbjct: 418 AAAAASSNYFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNFHWELVG-TDQAFAFPFVDF 476
Query: 71 PKGLLIKVQRRHLV 30
PKGL I+V++ ++
Sbjct: 477 PKGLPIRVKQHTVI 490
>ref|XP_002327809.1| predicted protein [Populus trichocarpa] gi|222836894|gb|EEE75287.1|
predicted protein [Populus trichocarpa]
Length = 488
Score = 672 bits (1735), Expect = 0.0
Identities = 333/432 (77%), Positives = 369/432 (85%)
Frame = -2
Query: 1325 MEQHISRYGKIYKSNLFGGPTIVSADADLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 1146
MEQHISRYGKIYKSNLFG PTIVSADA LNRFILQ+EG+LFECSYPRSIGGILGKWSMLV
Sbjct: 62 MEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQSEGRLFECSYPRSIGGILGKWSMLV 121
Query: 1145 LVGDMHRDMRNISLNFLSSARLRTHLLKEVESHTLFVLNSWKHDSTFCAQDEAKKFTFNL 966
LVGDMHR+MR+ISLNFLS ARLR+HLLKEVE TL VL+SWK + TF AQDEAKKFTFNL
Sbjct: 122 LVGDMHRNMRSISLNFLSHARLRSHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFNL 181
Query: 965 MAKHIMSLEPGKPETEKLKKEYITFMKGVVSAPLNFPGTAYRKALQSRSTILGFIEERMD 786
MAKH+MSL+PGKPETE+LKKEY+TFMKGVVSAPLNFPGT YRKAL+SRS IL FIE++M+
Sbjct: 182 MAKHMMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTPYRKALKSRSIILKFIEQKME 241
Query: 785 ERINQMKERNGDCTVENYDDDLLGWVLKHSNFMKDQILDLVLSLLFAGHETSSVSIALAI 606
ERI ++KE D +DDLLGWVLKHSN +QILDL+LSLLFAGHETSSVSIALAI
Sbjct: 242 ERIARLKEGVEDLE----EDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALAI 297
Query: 605 YFLQASPDALHQLREEHAEIQQGKKQSGEAELSWDDYKKMEFTHCVISETLRLGNVVRFL 426
YFLQA P A+ QL+EEH EI + KKQSGE E++WDDYKKMEFT CVISETLRLGNVVRFL
Sbjct: 298 YFLQACPGAIQQLKEEHIEISRAKKQSGEMEINWDDYKKMEFTQCVISETLRLGNVVRFL 357
Query: 425 HRKALKDVSYKGYDIPCGWKVLPVIAAVHLDASTFDNPQHFNPWRWSHQNTSEKPAPXXX 246
HRKA++DV YKGYDIPCGWKVLPVI+AVHLD++ FD PQ FNPWRW H N
Sbjct: 358 HRKAVRDVRYKGYDIPCGWKVLPVISAVHLDSTVFDQPQQFNPWRWQHNNARGSSTCSSA 417
Query: 245 XXXXSNVFMPFGGGPRLCTGSELAKLEMAVFIHHLVLNFNWELAGHSDHPFAFPFVDFPK 66
SN FMPFGGGPRLC GSELAKLEMAVFIHHLVLNF+WEL G +D FAFPFVDFPK
Sbjct: 418 AAASSNHFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNFHWELVG-ADQAFAFPFVDFPK 476
Query: 65 GLLIKVQRRHLV 30
GL I+V+ ++
Sbjct: 477 GLPIRVKHHTVI 488
>emb|CBI17531.3| unnamed protein product [Vitis vinifera]
Length = 488
Score = 672 bits (1734), Expect = 0.0
Identities = 338/430 (78%), Positives = 363/430 (84%)
Frame = -2
Query: 1325 MEQHISRYGKIYKSNLFGGPTIVSADADLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 1146
MEQHISR+G+IYKSNLFG PTIVSAD+ LNRFILQNEGKLFECSYPRSIGGILGKWSMLV
Sbjct: 62 MEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 121
Query: 1145 LVGDMHRDMRNISLNFLSSARLRTHLLKEVESHTLFVLNSWKHDSTFCAQDEAKKFTFNL 966
LVGDMHRDMR ISLNFLS RLRTHLL EV HTL VL+SWK + TF AQDEAKKFTFNL
Sbjct: 122 LVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKFTFNL 181
Query: 965 MAKHIMSLEPGKPETEKLKKEYITFMKGVVSAPLNFPGTAYRKALQSRSTILGFIEERMD 786
MAKHIMSL+PGKPETE+LKKEY+TFMKGVVSAPLNFPGTAYRKALQSRSTIL FIE +M+
Sbjct: 182 MAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIELKME 241
Query: 785 ERINQMKERNGDCTVENYDDDLLGWVLKHSNFMKDQILDLVLSLLFAGHETSSVSIALAI 606
ERI +++ G DDDLLGWVLKHSN +QILDLVLSLLFAGHETSSV+IALAI
Sbjct: 242 ERIQKLR---GGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALAI 298
Query: 605 YFLQASPDALHQLREEHAEIQQGKKQSGEAELSWDDYKKMEFTHCVISETLRLGNVVRFL 426
YFL+ P+A+ QLREEH EI + KKQSGE ELSW+DYKKMEFT CVISETLRLGNVVRFL
Sbjct: 299 YFLEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRFL 358
Query: 425 HRKALKDVSYKGYDIPCGWKVLPVIAAVHLDASTFDNPQHFNPWRWSHQNTSEKPAPXXX 246
HRKALKDV YKGYDIPCGWKVLPVIAAVHLD S FD PQHFNPWRW N + +
Sbjct: 359 HRKALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQQNNGNRGNSTSWS 418
Query: 245 XXXXSNVFMPFGGGPRLCTGSELAKLEMAVFIHHLVLNFNWELAGHSDHPFAFPFVDFPK 66
N FMPFGGGPRLC GSELAKLEMAVFIHHLVLN+NWEL D FAFPFVDFPK
Sbjct: 419 TATNQN-FMPFGGGPRLCAGSELAKLEMAVFIHHLVLNYNWELV-DKDQAFAFPFVDFPK 476
Query: 65 GLLIKVQRRH 36
GL IKV+ +
Sbjct: 477 GLPIKVRHHN 486
>ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-deoxocathasterone or
6-oxocampestanol to cathasterone [Populus trichocarpa]
gi|222853127|gb|EEE90674.1| cytochrome P450 probable
campestanol to 6-deoxocathasterone or 6-oxocampestanol to
cathasterone [Populus trichocarpa]
Length = 486
Score = 671 bits (1732), Expect = 0.0
Identities = 338/433 (78%), Positives = 368/433 (84%), Gaps = 1/433 (0%)
Frame = -2
Query: 1325 MEQHISRYGKIYKSNLFGGPTIVSADADLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 1146
MEQHISRYGKIYKSNLFG PTIVSADA LNRFILQNEG+LFECSYP+SIGGILGKWSMLV
Sbjct: 62 MEQHISRYGKIYKSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPKSIGGILGKWSMLV 121
Query: 1145 LVGDMHRDMRNISLNFLSSARLRTHLLKEVESHTLFVLNSWKHDSTFCAQDEAKKFTFNL 966
LVGDMHRDMR ISLNFLS ARLRTHLLKEVE TL VL+SWK + TF AQDEAKKFTFNL
Sbjct: 122 LVGDMHRDMRIISLNFLSHARLRTHLLKEVEKQTLLVLSSWKENCTFSAQDEAKKFTFNL 181
Query: 965 MAKHIMSLEPGKPETEKLKKEYITFMKGVVSAPLNFPGTAYRKALQSRSTILGFIEERMD 786
MAKHIMSL+PGKPETE+LKKEY+TFMKGVVSAP+N PGT YRKAL+SRS IL FIE +M+
Sbjct: 182 MAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPINLPGTPYRKALKSRSIILKFIERKME 241
Query: 785 ERINQMKERNGDCTVENY-DDDLLGWVLKHSNFMKDQILDLVLSLLFAGHETSSVSIALA 609
ERI + K VEN DDDLLGWVLKHSN +QILDL+LSLLFAGHETSSVSIALA
Sbjct: 242 ERIAETKGG-----VENLEDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVSIALA 296
Query: 608 IYFLQASPDALHQLREEHAEIQQGKKQSGEAELSWDDYKKMEFTHCVISETLRLGNVVRF 429
IYFLQA P A+ QL+EEH +I + KK+SGE EL+WDDYKKMEFT CVISETLRLGNVVRF
Sbjct: 297 IYFLQACPGAIQQLKEEHIQISRAKKRSGETELNWDDYKKMEFTQCVISETLRLGNVVRF 356
Query: 428 LHRKALKDVSYKGYDIPCGWKVLPVIAAVHLDASTFDNPQHFNPWRWSHQNTSEKPAPXX 249
LHRKALKDV YKGYDIPCGWKVLPVI+AVHLD++ FD PQHFNPWRW N + +
Sbjct: 357 LHRKALKDVRYKGYDIPCGWKVLPVISAVHLDSTLFDQPQHFNPWRWQQHNNARGASTSS 416
Query: 248 XXXXXSNVFMPFGGGPRLCTGSELAKLEMAVFIHHLVLNFNWELAGHSDHPFAFPFVDFP 69
SN FMPFGGGPRLC GSELAKLEMAVFIHHLVLNF+WEL +D FAFPFVDFP
Sbjct: 417 SGTTSSNYFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNFHWELV-DTDQAFAFPFVDFP 475
Query: 68 KGLLIKVQRRHLV 30
KGL I+V +HL+
Sbjct: 476 KGLPIRV--KHLL 486
>ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis vinifera]
Length = 487
Score = 671 bits (1731), Expect = 0.0
Identities = 338/430 (78%), Positives = 363/430 (84%)
Frame = -2
Query: 1325 MEQHISRYGKIYKSNLFGGPTIVSADADLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 1146
MEQHISR+G+IYKSNLFG PTIVSAD+ LNRFILQNEGKLFECSYPRSIGGILGKWSMLV
Sbjct: 62 MEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKWSMLV 121
Query: 1145 LVGDMHRDMRNISLNFLSSARLRTHLLKEVESHTLFVLNSWKHDSTFCAQDEAKKFTFNL 966
LVGDMHRDMR ISLNFLS RLRTHLL EV HTL VL+SWK + TF AQDEAKKFTFNL
Sbjct: 122 LVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKFTFNL 181
Query: 965 MAKHIMSLEPGKPETEKLKKEYITFMKGVVSAPLNFPGTAYRKALQSRSTILGFIEERMD 786
MAKHIMSL+PGKPETE+LKKEY+TFMKGVVSAPLNFPGTAYRKALQSRSTIL FIE +M+
Sbjct: 182 MAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIELKME 241
Query: 785 ERINQMKERNGDCTVENYDDDLLGWVLKHSNFMKDQILDLVLSLLFAGHETSSVSIALAI 606
ERI +++ G DDDLLGWVLKHSN +QILDLVLSLLFAGHETSSV+IALAI
Sbjct: 242 ERIQKLR---GGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALAI 298
Query: 605 YFLQASPDALHQLREEHAEIQQGKKQSGEAELSWDDYKKMEFTHCVISETLRLGNVVRFL 426
YFL+ P+A+ QLREEH EI + KKQSGE ELSW+DYKKMEFT CVISETLRLGNVVRFL
Sbjct: 299 YFLEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRFL 358
Query: 425 HRKALKDVSYKGYDIPCGWKVLPVIAAVHLDASTFDNPQHFNPWRWSHQNTSEKPAPXXX 246
HRKALKDV YKGYDIPCGWKVLPVIAAVHLD S FD PQHFNPWRW QN +
Sbjct: 359 HRKALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRW--QNNGNRGNSTSW 416
Query: 245 XXXXSNVFMPFGGGPRLCTGSELAKLEMAVFIHHLVLNFNWELAGHSDHPFAFPFVDFPK 66
+ FMPFGGGPRLC GSELAKLEMAVFIHHLVLN+NWEL D FAFPFVDFPK
Sbjct: 417 STATNQNFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNYNWELV-DKDQAFAFPFVDFPK 475
Query: 65 GLLIKVQRRH 36
GL IKV+ +
Sbjct: 476 GLPIKVRHHN 485