BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00030613
         (761 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003615678.1| Cytochrome P450 [Medicago truncatula] gi|355...   290   2e-76
gb|AAL66194.1|AF386512_1 cytochrome P450 [Pyrus communis]             280   2e-73
ref|XP_003615667.1| Cytochrome P450 [Medicago truncatula] gi|355...   278   1e-72
gb|AEY75215.1| cytochrome P450 CYP736A12 [Panax ginseng]              276   3e-72
dbj|BAD15331.1| cytochrome P450 [Panax ginseng]                       274   1e-71

>ref|XP_003615678.1| Cytochrome P450 [Medicago truncatula] gi|355517013|gb|AES98636.1|
           Cytochrome P450 [Medicago truncatula]
          Length = 510

 Score =  290 bits (743), Expect = 2e-76
 Identities = 146/253 (57%), Positives = 192/253 (75%)
 Frame = +1

Query: 1   VVDVSDVVGDLIEKMTYRMLFGKKHDDRFDLKGTMHHLMDQAGAFNIADYVPLLGPFDPQ 180
           VV+VS+VV +LIE + Y+++FG+   ++FDLK  +   +   GAFN+ADYVP LG FD Q
Sbjct: 173 VVNVSEVVENLIEDIVYKLMFGRSKYEQFDLKSLVQEGLALIGAFNLADYVPWLGIFDLQ 232

Query: 181 GLTKKIKAMHKAVDNILEIMISEHEQAAYIDRQEGHTTDFVDILLSVIDRSKEKTDEYYL 360
           GLTK  K + KA+D +LE++++EHEQAA +++ +    DFVDILLS++ ++ +   E  L
Sbjct: 233 GLTKSCKKVSKALDEVLEVILTEHEQAANVNKTQ---KDFVDILLSIMHQTIDIEGEQNL 289

Query: 361 NSIDREGIKAILIDMIAAGIDTSRTTVEWILSELVRHPRVMKKLQEEIKNVVGDKEMVED 540
             IDR  IKAIL+DMI A IDTS T++EW LSEL+RHPRVMKKLQ+EI+N VG+K MV +
Sbjct: 290 -VIDRTNIKAILLDMIVAAIDTSATSIEWALSELLRHPRVMKKLQDEIQNEVGNKRMVNE 348

Query: 541 KDLGKLHYLDLVIKESFRLHPAAPLLVPRESMEDTVIDGYHIPKKTRIFINTWALGRDPT 720
           KDL KL+YLD+V+ E+ RL+P APLLVPRES E T IDGY I +KTR+ +N WA+GRDP 
Sbjct: 349 KDLKKLNYLDMVVDETLRLYPVAPLLVPRESRESTTIDGYFIKEKTRLIVNAWAIGRDPN 408

Query: 721 VWSENVLEFIPER 759
           VWSEN  EF PER
Sbjct: 409 VWSENAKEFYPER 421


>gb|AAL66194.1|AF386512_1 cytochrome P450 [Pyrus communis]
          Length = 506

 Score =  280 bits (717), Expect = 2e-73
 Identities = 139/253 (54%), Positives = 182/253 (71%)
 Frame = +1

Query: 1   VVDVSDVVGDLIEKMTYRMLFGKKHDDRFDLKGTMHHLMDQAGAFNIADYVPLLGPFDPQ 180
           VVD S+ VG+L+E +TYRM+ G+K+DD FDLKG +   +   GAFNI+DYVP L P D Q
Sbjct: 170 VVDFSEKVGELVEGITYRMVLGRKNDDMFDLKGIIEEALFLTGAFNISDYVPFLSPLDLQ 229

Query: 181 GLTKKIKAMHKAVDNILEIMISEHEQAAYIDRQEGHTTDFVDILLSVIDRSKEKTDEYYL 360
           GLTK++K + K VD + E +I +HEQ +  + Q  H  DFVD+LLS I ++ +  DE  +
Sbjct: 230 GLTKRMKRVSKTVDQLFEKIIQDHEQVSRSE-QGNHHKDFVDVLLSSIHQTLKPNDEE-V 287

Query: 361 NSIDREGIKAILIDMIAAGIDTSRTTVEWILSELVRHPRVMKKLQEEIKNVVGDKEMVED 540
             ++R   KA L+DMIA   DTS T + W L+EL+RHP+VMK+LQEE+++V+G   MVE+
Sbjct: 288 YMLERTNAKATLLDMIAGAFDTSATAIIWTLAELLRHPKVMKRLQEELQSVIGMDRMVEE 347

Query: 541 KDLGKLHYLDLVIKESFRLHPAAPLLVPRESMEDTVIDGYHIPKKTRIFINTWALGRDPT 720
            DL KL YL +V+KESFRLHP APLLVP +SMED  +DGYH PKK+RIFIN W +GRDP 
Sbjct: 348 SDLPKLDYLSMVVKESFRLHPVAPLLVPHQSMEDITVDGYHTPKKSRIFINIWTIGRDPK 407

Query: 721 VWSENVLEFIPER 759
            W +N  EF PER
Sbjct: 408 SW-DNAEEFYPER 419


>ref|XP_003615667.1| Cytochrome P450 [Medicago truncatula] gi|355517002|gb|AES98625.1|
           Cytochrome P450 [Medicago truncatula]
          Length = 959

 Score =  278 bits (710), Expect = 1e-72
 Identities = 141/251 (56%), Positives = 189/251 (75%)
 Frame = +1

Query: 1   VVDVSDVVGDLIEKMTYRMLFGKKHDDRFDLKGTMHHLMDQAGAFNIADYVPLLGPFDPQ 180
           VV+VS++V ++IE + Y+M+ G+   ++FDLK  +   +   GAFN+ADYVP LG FD Q
Sbjct: 173 VVNVSEIVENVIEDIVYKMILGRGKYEQFDLKKLVQEGLALIGAFNLADYVPWLGIFDLQ 232

Query: 181 GLTKKIKAMHKAVDNILEIMISEHEQAAYIDRQEGHTTDFVDILLSVIDRSKEKTDEYYL 360
           GLTK  K + KA+D  LE++++EHEQAA +++   H  DFVDILLS++ ++ +   E  L
Sbjct: 233 GLTKSCKKVSKAIDEQLEVILTEHEQAANVNKT--HHKDFVDILLSIMHQTIDVEGEQNL 290

Query: 361 NSIDREGIKAILIDMIAAGIDTSRTTVEWILSELVRHPRVMKKLQEEIKNVVGDKEMVED 540
             IDR  IKAIL+DMI A IDTS T++EW LSEL+RHPRVMKKLQ+EI+N VG+K  +E+
Sbjct: 291 -VIDRTNIKAILLDMIVAAIDTSATSIEWALSELLRHPRVMKKLQDEIQNEVGNKRKIEE 349

Query: 541 KDLGKLHYLDLVIKESFRLHPAAPLLVPRESMEDTVIDGYHIPKKTRIFINTWALGRDPT 720
           KD+ KL+YLD+V+ E+ RL+P APLLVPRES E T+IDGY I +KTR+ +N WA+GRDP 
Sbjct: 350 KDMKKLNYLDMVVDETLRLYPVAPLLVPRESRESTIIDGYFIKEKTRLIVNAWAIGRDPN 409

Query: 721 VWSENVLEFIP 753
           VWSEN  E IP
Sbjct: 410 VWSEN-FESIP 419



 Score =  258 bits (659), Expect = 1e-66
 Identities = 134/253 (52%), Positives = 178/253 (70%)
 Frame = +1

Query: 1    VVDVSDVVGDLIEKMTYRMLFGKKHDDRFDLKGTMHHLMDQAGAFNIADYVPLLGPFDPQ 180
            VV+VS++V +LIE + Y+M+ G+   ++FDLK  +   +   GAFN+ADYVP LG FD Q
Sbjct: 623  VVNVSEIVENLIEDIVYKMILGRGKYEQFDLKKMIKDGLTLMGAFNLADYVPWLGIFDLQ 682

Query: 181  GLTKKIKAMHKAVDNILEIMISEHEQAAYIDRQEGHTTDFVDILLSVIDRSKEKTDEYYL 360
            GLT+  K   KA+D +LE++I+EHEQ    D  +    DFVD +LS++ ++ +      L
Sbjct: 683  GLTQACKKTSKALDEVLEMIITEHEQTTNTDDPK----DFVDTVLSIMHQTIDVEGGQDL 738

Query: 361  NSIDREGIKAILIDMIAAGIDTSRTTVEWILSELVRHPRVMKKLQEEIKNVVGDKEMVED 540
              IDR  IKAIL+DMI A IDTS   +EW LSEL+RHPRVMK LQ+EI+N VG+K MVE+
Sbjct: 739  -VIDRTIIKAILLDMIGASIDTSSNVIEWALSELLRHPRVMKILQDEIQNEVGNKRMVEE 797

Query: 541  KDLGKLHYLDLVIKESFRLHPAAPLLVPRESMEDTVIDGYHIPKKTRIFINTWALGRDPT 720
            KDL   +YLD+V+ E+ RL+P APLL+PRE  E+  ID Y I +KTR+ +N WA+GRDP 
Sbjct: 798  KDLKNFNYLDMVVDETLRLYPVAPLLIPRECRENITIDDYSIKEKTRVIVNAWAIGRDPD 857

Query: 721  VWSENVLEFIPER 759
            VWSEN  EF PER
Sbjct: 858  VWSENAEEFYPER 870


>gb|AEY75215.1| cytochrome P450 CYP736A12 [Panax ginseng]
          Length = 500

 Score =  276 bits (707), Expect = 3e-72
 Identities = 141/253 (55%), Positives = 179/253 (70%)
 Frame = +1

Query: 1   VVDVSDVVGDLIEKMTYRMLFGKKHDDRFDLKGTMHHLMDQAGAFNIADYVPLLGPFDPQ 180
           VVD+S  V ++IE MTYR+L G+  DDR+DLKG M+  +  AG FNIAD+VP LGP D Q
Sbjct: 169 VVDLSAKVANIIENMTYRLLLGRTKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQ 228

Query: 181 GLTKKIKAMHKAVDNILEIMISEHEQAAYIDRQEGHTTDFVDILLSVIDRSKEKTDEYYL 360
           GLT++ K   K +D ILE +I EHEQ +      G   DF+D +LS+ ++     DE   
Sbjct: 229 GLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASG---DFIDDMLSLKNKPSNTHDELS- 284

Query: 361 NSIDREGIKAILIDMIAAGIDTSRTTVEWILSELVRHPRVMKKLQEEIKNVVGDKEMVED 540
             IDR  IKAI+ID+I+A IDTS T++EWIL+EL++HPR MKK QEEI  VVG   MVE+
Sbjct: 285 KVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEE 344

Query: 541 KDLGKLHYLDLVIKESFRLHPAAPLLVPRESMEDTVIDGYHIPKKTRIFINTWALGRDPT 720
            DL  L Y+ +V+KE  RLHP APLL P ESMED  I+GY IPK++R+ +N+WALGRDP 
Sbjct: 345 TDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPN 404

Query: 721 VWSENVLEFIPER 759
           VWSEN  EF+PER
Sbjct: 405 VWSENAEEFLPER 417


>dbj|BAD15331.1| cytochrome P450 [Panax ginseng]
          Length = 500

 Score =  274 bits (701), Expect = 1e-71
 Identities = 140/253 (55%), Positives = 179/253 (70%)
 Frame = +1

Query: 1   VVDVSDVVGDLIEKMTYRMLFGKKHDDRFDLKGTMHHLMDQAGAFNIADYVPLLGPFDPQ 180
           VVD+S  V ++IE MTYR+L G+  DDR+DLKG M+  +  AG FNIAD+VP LGP D Q
Sbjct: 169 VVDLSAKVANIIENMTYRLLLGRTKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQ 228

Query: 181 GLTKKIKAMHKAVDNILEIMISEHEQAAYIDRQEGHTTDFVDILLSVIDRSKEKTDEYYL 360
           GLT++ K   K +D ILE +I EHEQ +      G   DF+D +LS+ ++     DE   
Sbjct: 229 GLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASG---DFIDDMLSLKNKPSNTHDELS- 284

Query: 361 NSIDREGIKAILIDMIAAGIDTSRTTVEWILSELVRHPRVMKKLQEEIKNVVGDKEMVED 540
             IDR  IKAI+ID+I+A IDTS T++EWIL+EL++HPR MKK QEEI  VVG   MVE+
Sbjct: 285 KVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEE 344

Query: 541 KDLGKLHYLDLVIKESFRLHPAAPLLVPRESMEDTVIDGYHIPKKTRIFINTWALGRDPT 720
            DL  L Y+ +V+KE  RLHP APLL P ESMED  I+GY IPK++R+ +N+WALGRDP 
Sbjct: 345 TDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPN 404

Query: 721 VWSENVLEFIPER 759
           VWSE+  EF+PER
Sbjct: 405 VWSEDADEFLPER 417