BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00030273
         (1262 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002518890.1| kinase, putative [Ricinus communis] gi|22354...   342   2e-91
emb|CBI34567.3| unnamed protein product [Vitis vinifera]              338   2e-90
ref|XP_002273005.2| PREDICTED: serine/threonine-protein kinase W...   338   2e-90
ref|XP_003601461.1| Serine/threonine protein kinase WNK8 [Medica...   336   7e-90
emb|CBI40585.3| unnamed protein product [Vitis vinifera]              334   2e-89

>ref|XP_002518890.1| kinase, putative [Ricinus communis] gi|223541877|gb|EEF43423.1|
           kinase, putative [Ricinus communis]
          Length = 585

 Score =  342 bits (876), Expect = 2e-91
 Identities = 168/281 (59%), Positives = 204/281 (72%), Gaps = 4/281 (1%)
 Frame = -3

Query: 879 DPDEDVIVELSPNNRYIRYQEALSFGAYKIVYKGFDKDNGTEVVWNKFSLTDLALNSPTH 700
           D D+    E  P  RY+RY E L  GA+K VYK FD+ NG EV WN+ S+ D+ L SP  
Sbjct: 7   DDDDSEFEEKDPTGRYVRYDEVLGKGAFKTVYKAFDEINGIEVAWNQVSVEDV-LQSPDQ 65

Query: 699 CQRLVLEAANIKLLKHGNIARCYDSWVDTENKTVNMITESCCSGSLREFRMKHKGVDMKA 520
            +RL  E   +K LKH NI + Y+SWVD  NKT+NMITE   SGSLR++R KHK VD+KA
Sbjct: 66  LERLYSEVHLLKSLKHENIMKFYNSWVDDNNKTINMITELFTSGSLRKYRKKHKNVDIKA 125

Query: 519 IKKWARQILQGLCYLHSHNPPVVHGDLNCDNVFINGNGGEVKIGGLGFLTIL----GRGV 352
           IK WARQIL+GL YLHSHNPP++H DL CDNVF+NGN GEVKIG LG  T++     R V
Sbjct: 126 IKNWARQILRGLHYLHSHNPPIIHRDLKCDNVFVNGNNGEVKIGDLGLATVMQQPTARSV 185

Query: 351 AGTFKPEFMAPEVFEGASDQLVDIYAFGMCVLELVTCEYPYSECKNPGQRYRKIVSGGKP 172
            GT  PEFMAPE++E   ++LVDIY+FGMC+LE+VTCEYPYSECKNP Q Y+K+ SG KP
Sbjct: 186 IGT--PEFMAPELYEEEYNELVDIYSFGMCILEMVTCEYPYSECKNPAQIYKKVTSGIKP 243

Query: 171 DALGRVKNMQAKDFIEKCLVPASQRMSAAELLNDPFLSCEN 49
            +L +V +   K FIEKC+VPAS R+ A ELL DPFL+ EN
Sbjct: 244 ASLCKVNDPLVKRFIEKCIVPASMRLPALELLKDPFLATEN 284


>emb|CBI34567.3| unnamed protein product [Vitis vinifera]
          Length = 596

 Score =  338 bits (867), Expect = 2e-90
 Identities = 165/287 (57%), Positives = 206/287 (71%), Gaps = 4/287 (1%)
 Frame = -3

Query: 873 DEDVIVELSPNNRYIRYQEALSFGAYKIVYKGFDKDNGTEVVWNKFSLTDLALNSPTHCQ 694
           D+    E  P  RY+RY E L  GA+K VYK FD+ +G EV W +  + DL L SP   +
Sbjct: 148 DDSDFEEKDPTGRYVRYNEFLGKGAFKTVYKAFDEVDGIEVAWGQVEIEDL-LQSPQQLE 206

Query: 693 RLVLEAANIKLLKHGNIARCYDSWVDTENKTVNMITESCCSGSLREFRMKHKGVDMKAIK 514
           RL  E   +K LKH NI + Y+SWVD  NKT+N+ITE   SGSLR++R KHK VD+KAIK
Sbjct: 207 RLYSEVHLLKSLKHDNIIKFYNSWVDDTNKTINLITELFTSGSLRQYRKKHKNVDLKAIK 266

Query: 513 KWARQILQGLCYLHSHNPPVVHGDLNCDNVFINGNGGEVKIGGLGFLTIL----GRGVAG 346
            WA+QIL+GL YLHSHNPP++H DL CDN+F+NGN GEVKIG LG   ++     R V G
Sbjct: 267 NWAKQILRGLHYLHSHNPPIIHRDLKCDNIFVNGNNGEVKIGDLGLAIVMQQPTARSVIG 326

Query: 345 TFKPEFMAPEVFEGASDQLVDIYAFGMCVLELVTCEYPYSECKNPGQRYRKIVSGGKPDA 166
           T  PEFMAPE++E   ++LVDIY+FGMC+LELVTCEYPY+ECKNP Q Y+K+ SG KP +
Sbjct: 327 T--PEFMAPELYEEEYNELVDIYSFGMCILELVTCEYPYNECKNPAQIYKKVSSGIKPAS 384

Query: 165 LGRVKNMQAKDFIEKCLVPASQRMSAAELLNDPFLSCENLNDPLKLT 25
           LG+V + Q K FIEKCLVPAS R+SA ELL D F + EN  +PL ++
Sbjct: 385 LGKVSDPQVKQFIEKCLVPASLRLSAQELLKDAFFATENSKEPLSVS 431


>ref|XP_002273005.2| PREDICTED: serine/threonine-protein kinase WNK8-like [Vitis
           vinifera]
          Length = 645

 Score =  338 bits (866), Expect = 2e-90
 Identities = 165/288 (57%), Positives = 206/288 (71%), Gaps = 4/288 (1%)
 Frame = -3

Query: 885 KSDPDEDVIVELSPNNRYIRYQEALSFGAYKIVYKGFDKDNGTEVVWNKFSLTDLALNSP 706
           K + D+    E  P  RY+RY E L  GA+K VYK FD+ +G EV W +  + DL L SP
Sbjct: 5   KFETDDSDFEEKDPTGRYVRYNEFLGKGAFKTVYKAFDEVDGIEVAWGQVEIEDL-LQSP 63

Query: 705 THCQRLVLEAANIKLLKHGNIARCYDSWVDTENKTVNMITESCCSGSLREFRMKHKGVDM 526
              +RL  E   +K LKH NI + Y+SWVD  NKT+N+ITE   SGSLR++R KHK VD+
Sbjct: 64  QQLERLYSEVHLLKSLKHDNIIKFYNSWVDDTNKTINLITELFTSGSLRQYRKKHKNVDL 123

Query: 525 KAIKKWARQILQGLCYLHSHNPPVVHGDLNCDNVFINGNGGEVKIGGLGFLTIL----GR 358
           KAIK WA+QIL+GL YLHSHNPP++H DL CDN+F+NGN GEVKIG LG   ++     R
Sbjct: 124 KAIKNWAKQILRGLHYLHSHNPPIIHRDLKCDNIFVNGNNGEVKIGDLGLAIVMQQPTAR 183

Query: 357 GVAGTFKPEFMAPEVFEGASDQLVDIYAFGMCVLELVTCEYPYSECKNPGQRYRKIVSGG 178
            V GT  PEFMAPE++E   ++LVDIY+FGMC+LELVTCEYPY+ECKNP Q Y+K+ SG 
Sbjct: 184 SVIGT--PEFMAPELYEEEYNELVDIYSFGMCILELVTCEYPYNECKNPAQIYKKVSSGI 241

Query: 177 KPDALGRVKNMQAKDFIEKCLVPASQRMSAAELLNDPFLSCENLNDPL 34
           KP +LG+V + Q K FIEKCLVPAS R+SA ELL D F + EN  +P+
Sbjct: 242 KPASLGKVSDPQVKQFIEKCLVPASLRLSAQELLKDAFFATENSKEPV 289


>ref|XP_003601461.1| Serine/threonine protein kinase WNK8 [Medicago truncatula]
           gi|355490509|gb|AES71712.1| Serine/threonine protein
           kinase WNK8 [Medicago truncatula]
          Length = 614

 Score =  336 bits (862), Expect = 7e-90
 Identities = 161/285 (56%), Positives = 207/285 (72%), Gaps = 4/285 (1%)
 Frame = -3

Query: 882 SDPDEDVIVELSPNNRYIRYQEALSFGAYKIVYKGFDKDNGTEVVWNKFSLTDLALNSPT 703
           S+ D+  +VE  P  RY RY E L  GA+K VYK FD+ +G EV WN+ S+ D+ + SP 
Sbjct: 3   SEIDDVGVVEKDPTLRYARYDEMLGKGAFKTVYKAFDEVDGIEVAWNQISIEDV-MQSPQ 61

Query: 702 HCQRLVLEAANIKLLKHGNIARCYDSWVDTENKTVNMITESCCSGSLREFRMKHKGVDMK 523
           + ++L  E   +K LKH NI + Y SWVD ++  +NMITE   SGSLR++R KHK VDMK
Sbjct: 62  NLEKLYSEVHLLKSLKHENIIKMYSSWVDEKSSNINMITELFTSGSLRQYRKKHKNVDMK 121

Query: 522 AIKKWARQILQGLCYLHSHNPPVVHGDLNCDNVFINGNGGEVKIGGLGFLTIL----GRG 355
           AIK WARQIL+GLC+LHSHNPPV+H DL CDN+F+NGN G+VKIG LG   ++     R 
Sbjct: 122 AIKNWARQILRGLCFLHSHNPPVIHRDLKCDNIFVNGNNGQVKIGDLGLAIVMQQPTARS 181

Query: 354 VAGTFKPEFMAPEVFEGASDQLVDIYAFGMCVLELVTCEYPYSECKNPGQRYRKIVSGGK 175
           V GT  PEFMAPE++E   ++LVD+Y+FGMC+LE++TCEYPYSECKNP Q Y+K+ SG K
Sbjct: 182 VIGT--PEFMAPELYEEEYNELVDVYSFGMCILEMITCEYPYSECKNPAQIYKKVTSGIK 239

Query: 174 PDALGRVKNMQAKDFIEKCLVPASQRMSAAELLNDPFLSCENLND 40
           P +L RV + + K FIEKCLVPAS R+ A+ELL DPFL+  N  +
Sbjct: 240 PASLARVNDPEVKQFIEKCLVPASMRLPASELLKDPFLATGNTKE 284


>emb|CBI40585.3| unnamed protein product [Vitis vinifera]
          Length = 591

 Score =  334 bits (857), Expect = 2e-89
 Identities = 162/295 (54%), Positives = 210/295 (71%), Gaps = 4/295 (1%)
 Frame = -3

Query: 879 DPDEDVIVELSPNNRYIRYQEALSFGAYKIVYKGFDKDNGTEVVWNKFSLTDLALNSPTH 700
           D DE   VE  P  RY+R++E L  GA+K VYK FD+ +G E+ WN+  + D+ L SP  
Sbjct: 14  DEDEAAYVEKDPTGRYVRFKEILGKGAFKTVYKAFDEVDGIEIAWNQVKIDDV-LRSPED 72

Query: 699 CQRLVLEAANIKLLKHGNIARCYDSWVDTENKTVNMITESCCSGSLREFRMKHKGVDMKA 520
            ++L  E   +K LKH NI + Y+SWVD + KTVNMITE   SGSLR++R KHK VDMKA
Sbjct: 73  LEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKNVDMKA 132

Query: 519 IKKWARQILQGLCYLHSHNPPVVHGDLNCDNVFINGNGGEVKIGGLGFLTIL----GRGV 352
           IK WARQ+L+GL YLHSHNPP++H DL CDN+F+NGN GEVKIG LG   ++     R V
Sbjct: 133 IKNWARQVLRGLVYLHSHNPPIIHRDLKCDNIFVNGNHGEVKIGDLGLAIVMQQPTARSV 192

Query: 351 AGTFKPEFMAPEVFEGASDQLVDIYAFGMCVLELVTCEYPYSECKNPGQRYRKIVSGGKP 172
            GT  PEFMAPE++E   ++LVDIY+FGMC+LE+VT EYPY+ECKNP Q Y+K+ SG KP
Sbjct: 193 IGT--PEFMAPELYEEEYNELVDIYSFGMCMLEMVTFEYPYNECKNPAQIYKKVTSGIKP 250

Query: 171 DALGRVKNMQAKDFIEKCLVPASQRMSAAELLNDPFLSCENLNDPLKLTVRNLLP 7
            +L +V ++Q K+FI KCL PAS+R+ A ELL DPF   EN  +P+++ +    P
Sbjct: 251 ASLCKVTDLQIKEFIVKCLAPASERLPAKELLKDPFFQSENPKEPIRVPLHPDFP 305