BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00030162
         (886 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAT92094.1| GA 2-oxidase 2 [Nerium oleander]                       412   e-113
gb|AFH56955.1| gibberellin 2-oxidase 2 [Petunia x hybrida]            404   e-110
ref|XP_002300430.1| gibberellin 2-oxidase [Populus trichocarpa] ...   404   e-110
ref|XP_002316789.1| gibberellin 2-oxidase [Populus trichocarpa] ...   402   e-110
gb|AFP58845.1| gibberellin 2-oxidase [Populus tomentosa]              396   e-108

>gb|AAT92094.1| GA 2-oxidase 2 [Nerium oleander]
          Length = 332

 Score =  412 bits (1060), Expect = e-113
 Identities = 205/279 (73%), Positives = 237/279 (84%), Gaps = 5/279 (1%)
 Frame = -1

Query: 823 MVVMSKQPQTKMEQFCAVNSSQITTTL--IPLIDLSQPDSKACLVKACEEFGFFKVINHG 650
           MVV+SK     MEQFC V + +  T    +PLIDLS+PDSK  LVKAC+EFGFFKVINHG
Sbjct: 1   MVVLSKPA---MEQFCLVKNCKAPTLFPGVPLIDLSKPDSKHLLVKACQEFGFFKVINHG 57

Query: 649 IPTDFISKLESQAVKFFSSPMSDKEKAGPPDPCGYGNKSIGRNGDIGWVEYLLLTTNSES 470
           +PT+FI+KLES+AVKFFS P+SDKEKAGPPDP GYGNK IG NGDIGWVEY++L+TNSE 
Sbjct: 58  VPTEFITKLESEAVKFFSLPLSDKEKAGPPDPFGYGNKKIGPNGDIGWVEYIILSTNSEF 117

Query: 469 DYSRIAAVFGETPENFSLCVNNYVKAVKKMACDILEMLADGLMIQEKNVFSELLMDKQSD 290
           +Y R  ++FG TPE F   VN+YV +VKKMAC+ILE++A+GL IQ KNVFS+LLMD+QSD
Sbjct: 118 NYQRFESIFGMTPEKFRDAVNDYVSSVKKMACEILELMAEGLNIQPKNVFSKLLMDEQSD 177

Query: 289 SVFRLNHYPPCP---VVHGKNLIGFGEHTDPQIISVLRSNNTSGLQISLREGSWISVPAD 119
           SVFRLNHYPP P    +HG+NLIGFGEHTDPQIISVLRSNNTSGLQI L++GSWIS+P D
Sbjct: 178 SVFRLNHYPPYPDLQELHGRNLIGFGEHTDPQIISVLRSNNTSGLQIQLKDGSWISIPPD 237

Query: 118 QDSFFINVGDSLQVMTNGRFKSVKHRVLANSYKSRVSMI 2
           Q SFFINVGDS QV+TNGRFKSVKHRVLANS KSR+SMI
Sbjct: 238 QSSFFINVGDSTQVLTNGRFKSVKHRVLANSMKSRLSMI 276


>gb|AFH56955.1| gibberellin 2-oxidase 2 [Petunia x hybrida]
          Length = 332

 Score =  404 bits (1039), Expect = e-110
 Identities = 202/279 (72%), Positives = 237/279 (84%), Gaps = 5/279 (1%)
 Frame = -1

Query: 823 MVVMSKQPQTKMEQFCAVNSSQITTTL--IPLIDLSQPDSKACLVKACEEFGFFKVINHG 650
           MVV+SK     +E F  VN+ +  +    +PLIDLS+PDSK  +VKACEEFGFFKVINHG
Sbjct: 1   MVVLSKPA---IEHFSIVNNCKPPSYFSGVPLIDLSKPDSKNLIVKACEEFGFFKVINHG 57

Query: 649 IPTDFISKLESQAVKFFSSPMSDKEKAGPPDPCGYGNKSIGRNGDIGWVEYLLLTTNSES 470
           +PT+FISKLES+A+KFFSSP+S+KEKAGP DP GYGNK IG NGD+GWVEY+L++TNSE 
Sbjct: 58  VPTEFISKLESEAIKFFSSPLSEKEKAGPADPFGYGNKKIGSNGDVGWVEYILVSTNSEF 117

Query: 469 DYSRIAAVFGETPENFSLCVNNYVKAVKKMACDILEMLADGLMIQEKNVFSELLMDKQSD 290
           DY++ A++ G   EN    VN+YV AVKKMAC ILE+LA+GL IQ++NVFS+LLMD++SD
Sbjct: 118 DYNKFASILGVNSENIRDAVNDYVSAVKKMACQILEVLAEGLKIQQRNVFSKLLMDEKSD 177

Query: 289 SVFRLNHYPPCPVVH---GKNLIGFGEHTDPQIISVLRSNNTSGLQISLREGSWISVPAD 119
           SVFRLNHYPPCP V    GKNLIGFGEHTDPQIISVLRSNNTSGLQISL++G WISVP D
Sbjct: 178 SVFRLNHYPPCPEVEEFSGKNLIGFGEHTDPQIISVLRSNNTSGLQISLKDGHWISVPPD 237

Query: 118 QDSFFINVGDSLQVMTNGRFKSVKHRVLANSYKSRVSMI 2
           Q+SFFINVGDSLQVMTNGRFKSV+HRVLANS KSR+SMI
Sbjct: 238 QNSFFINVGDSLQVMTNGRFKSVRHRVLANSVKSRLSMI 276


>ref|XP_002300430.1| gibberellin 2-oxidase [Populus trichocarpa]
           gi|222847688|gb|EEE85235.1| gibberellin 2-oxidase
           [Populus trichocarpa]
          Length = 332

 Score =  404 bits (1037), Expect = e-110
 Identities = 200/279 (71%), Positives = 235/279 (84%), Gaps = 5/279 (1%)
 Frame = -1

Query: 823 MVVMSKQPQTKMEQFCAVNSSQITTTL--IPLIDLSQPDSKACLVKACEEFGFFKVINHG 650
           MV++SK     +EQF  + + + TT    IPLIDLS+PDSK  LV+ACEEFGFFKVINHG
Sbjct: 1   MVLISKPA---LEQFSFIRNRKPTTVFSGIPLIDLSKPDSKHLLVEACEEFGFFKVINHG 57

Query: 649 IPTDFISKLESQAVKFFSSPMSDKEKAGPPDPCGYGNKSIGRNGDIGWVEYLLLTTNSES 470
           +P +FISKLES+AV FFS P+S+KEK GPP P GYGNKSIG+NGD+GWVEYLLLTTN ES
Sbjct: 58  VPMEFISKLESEAVNFFSLPLSEKEKVGPPSPFGYGNKSIGQNGDVGWVEYLLLTTNQES 117

Query: 469 DYSRIAAVFGETPENFSLCVNNYVKAVKKMACDILEMLADGLMIQEKNVFSELLMDKQSD 290
              R ++VFG+ PE F   +N+YV AVKKMAC+ILEM+ADGL +Q++NVFS+LLMD+QSD
Sbjct: 118 ISQRFSSVFGDNPEKFRCALNDYVSAVKKMACEILEMMADGLKLQQRNVFSKLLMDEQSD 177

Query: 289 SVFRLNHYPPCPVVHG---KNLIGFGEHTDPQIISVLRSNNTSGLQISLREGSWISVPAD 119
           SVFRLNHYPPCP +     +N+IGFGEHTDPQIISVLRSNNTSGLQISL +GSWISVP D
Sbjct: 178 SVFRLNHYPPCPEIEALTDQNMIGFGEHTDPQIISVLRSNNTSGLQISLSDGSWISVPPD 237

Query: 118 QDSFFINVGDSLQVMTNGRFKSVKHRVLANSYKSRVSMI 2
           Q+SFF+NVGDSLQVMTNGRFKSV+HRVL NS K+RVSMI
Sbjct: 238 QNSFFVNVGDSLQVMTNGRFKSVRHRVLTNSMKARVSMI 276


>ref|XP_002316789.1| gibberellin 2-oxidase [Populus trichocarpa]
           gi|222859854|gb|EEE97401.1| gibberellin 2-oxidase
           [Populus trichocarpa]
          Length = 332

 Score =  402 bits (1032), Expect = e-110
 Identities = 197/272 (72%), Positives = 230/272 (84%), Gaps = 5/272 (1%)
 Frame = -1

Query: 802 PQTKMEQFCAVNSSQITTTL--IPLIDLSQPDSKACLVKACEEFGFFKVINHGIPTDFIS 629
           P+  ++QF  + + + TT    IPLIDLS+PDSK  LVKACEEFGFFKV+NHG+P +FIS
Sbjct: 5   PKPALQQFSFIRNIKPTTFFSGIPLIDLSKPDSKHLLVKACEEFGFFKVVNHGVPLEFIS 64

Query: 628 KLESQAVKFFSSPMSDKEKAGPPDPCGYGNKSIGRNGDIGWVEYLLLTTNSESDYSRIAA 449
           KLES+AVKFFS P+S+KEKA PP+P GYG KSIG+NGD+GWVEYLLLTTN ES   R+++
Sbjct: 65  KLESEAVKFFSLPLSEKEKASPPNPFGYGKKSIGQNGDVGWVEYLLLTTNQESVSQRLSS 124

Query: 448 VFGETPENFSLCVNNYVKAVKKMACDILEMLADGLMIQEKNVFSELLMDKQSDSVFRLNH 269
           VFG+ PE F   +N+YV AVKKMAC+ILEM+ADGL IQ +NVFS+LLMD+QSDSVFRLNH
Sbjct: 125 VFGDNPEKFRCALNDYVSAVKKMACEILEMMADGLKIQNRNVFSKLLMDEQSDSVFRLNH 184

Query: 268 YPPCPVVHG---KNLIGFGEHTDPQIISVLRSNNTSGLQISLREGSWISVPADQDSFFIN 98
           YPPCP +      N+IGFGEHTDPQIISVLRSNNTSGLQISL +GSWISVP D  SFFIN
Sbjct: 185 YPPCPEIQALKDHNMIGFGEHTDPQIISVLRSNNTSGLQISLNDGSWISVPPDPSSFFIN 244

Query: 97  VGDSLQVMTNGRFKSVKHRVLANSYKSRVSMI 2
           VGDSLQVMTNGRFKSV+HRVLANS K+R+SMI
Sbjct: 245 VGDSLQVMTNGRFKSVRHRVLANSIKARISMI 276


>gb|AFP58845.1| gibberellin 2-oxidase [Populus tomentosa]
          Length = 332

 Score =  396 bits (1018), Expect = e-108
 Identities = 193/268 (72%), Positives = 229/268 (85%), Gaps = 5/268 (1%)
 Frame = -1

Query: 790 MEQFCAVNSSQITTTL--IPLIDLSQPDSKACLVKACEEFGFFKVINHGIPTDFISKLES 617
           ++QF  + + + TT    IPLIDLS+PDSK  LVKACEEFGFFKV+NHG+P +FISKLES
Sbjct: 9   LQQFSFIRNIKPTTFFSGIPLIDLSKPDSKHLLVKACEEFGFFKVVNHGVPLEFISKLES 68

Query: 616 QAVKFFSSPMSDKEKAGPPDPCGYGNKSIGRNGDIGWVEYLLLTTNSESDYSRIAAVFGE 437
           +AVKFFS P S+KEKA PP+P GYG KSIG+NGD+GWVEYLLLT N ES   R+++VFG+
Sbjct: 69  EAVKFFSLPPSEKEKASPPNPFGYGKKSIGQNGDVGWVEYLLLTANQESVSQRLSSVFGD 128

Query: 436 TPENFSLCVNNYVKAVKKMACDILEMLADGLMIQEKNVFSELLMDKQSDSVFRLNHYPPC 257
            PE F   +N+YV AVKKMAC+ILEM+ADGL +Q++NVFS+LLMD+QSDSVFRLNHYPPC
Sbjct: 129 NPEKFRYALNDYVSAVKKMACEILEMMADGLKLQQRNVFSKLLMDEQSDSVFRLNHYPPC 188

Query: 256 PVVHG---KNLIGFGEHTDPQIISVLRSNNTSGLQISLREGSWISVPADQDSFFINVGDS 86
           P +     +N+IGFGEHTDPQIIS+LRSNNTSGLQISL +GSWISVP DQ+SFF+NVGDS
Sbjct: 189 PEIEALTDQNMIGFGEHTDPQIISLLRSNNTSGLQISLSDGSWISVPPDQNSFFVNVGDS 248

Query: 85  LQVMTNGRFKSVKHRVLANSYKSRVSMI 2
           LQVMTNGRFKSV+HRVL NS K+RVSMI
Sbjct: 249 LQVMTNGRFKSVRHRVLTNSMKARVSMI 276