BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00030046
         (666 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI40195.3| unnamed protein product [Vitis vinifera]              236   3e-60
ref|XP_002262782.2| PREDICTED: dihydrolipoyllysine-residue acety...   236   4e-60
ref|XP_002526756.1| dihydrolipoamide acetyltransferase component...   214   2e-53
ref|XP_004138681.1| PREDICTED: dihydrolipoyllysine-residue acety...   195   6e-48
ref|XP_004159237.1| PREDICTED: dihydrolipoyllysine-residue acety...   195   7e-48

>emb|CBI40195.3| unnamed protein product [Vitis vinifera]
          Length = 659

 Score =  236 bits (602), Expect = 3e-60
 Identities = 121/176 (68%), Positives = 145/176 (82%)
 Frame = +3

Query: 135 QMALSRLRHPVICRAPSLFKSRSLSSITVASRYLASSSGLKISSIDVDETLLRPTALSGY 314
           +MALSRLRHP++ RAPSLF++R LSS   ASR L  +S ++ SS+D D TLLRP +L   
Sbjct: 23  KMALSRLRHPIVSRAPSLFRARILSS--TASRSLPHTSTVQKSSVDGDGTLLRPASLLMV 80

Query: 315 THISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEVGDV 494
             + + SS +KLQ+G+R+FSS E PSH+V+ MPALSPTMTQGNIAKW+KKEGDKIE GDV
Sbjct: 81  PRVQDGSSKLKLQVGVRNFSSAELPSHMVLGMPALSPTMTQGNIAKWRKKEGDKIEPGDV 140

Query: 495 ICEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNI 662
           +CEIETDKATLEFE LEEGFLAKIL  EGSKDVPVGQPIAITVED +DI  VP+++
Sbjct: 141 LCEIETDKATLEFESLEEGFLAKILVAEGSKDVPVGQPIAITVEDEEDIQKVPASV 196



 Score =  160 bits (405), Expect = 2e-37
 Identities = 91/163 (55%), Positives = 113/163 (69%), Gaps = 9/163 (5%)
 Frame = +3

Query: 204 LSSITVA--SRYLASSSGLKISSIDVDETLLRPTALSGYTHISEKSSSI-------KLQI 356
           L+ I VA  S+ +     + I+  D ++    P +++G + + EK S         K Q 
Sbjct: 161 LAKILVAEGSKDVPVGQPIAITVEDEEDIQKVPASVAGGSGVEEKKSKHENAGNEDKQQE 220

Query: 357 GLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEVGDVICEIETDKATLEFE 536
                ++ E P HIV+ MPALSPTM QGNIAKW+KKEGDKIEVGDVICEIETDKATLEFE
Sbjct: 221 MSSTINTAELPPHIVLGMPALSPTMNQGNIAKWRKKEGDKIEVGDVICEIETDKATLEFE 280

Query: 537 CLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNIT 665
            LEEG+LAKI+APEGSKDV VGQPIAITVED DDI  V ++++
Sbjct: 281 SLEEGYLAKIVAPEGSKDVAVGQPIAITVEDPDDIEIVKASVS 323


>ref|XP_002262782.2| PREDICTED: dihydrolipoyllysine-residue acetyltransferase component
           1 of pyruvate dehydrogenase complex, mitochondrial-like
           [Vitis vinifera]
          Length = 636

 Score =  236 bits (601), Expect = 4e-60
 Identities = 121/175 (69%), Positives = 144/175 (82%)
 Frame = +3

Query: 138 MALSRLRHPVICRAPSLFKSRSLSSITVASRYLASSSGLKISSIDVDETLLRPTALSGYT 317
           MALSRLRHP++ RAPSLF++R LSS   ASR L  +S ++ SS+D D TLLRP +L    
Sbjct: 1   MALSRLRHPIVSRAPSLFRARILSS--TASRSLPHTSTVQKSSVDGDGTLLRPASLLMVP 58

Query: 318 HISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEVGDVI 497
            + + SS +KLQ+G+R+FSS E PSH+V+ MPALSPTMTQGNIAKW+KKEGDKIE GDV+
Sbjct: 59  RVQDGSSKLKLQVGVRNFSSAELPSHMVLGMPALSPTMTQGNIAKWRKKEGDKIEPGDVL 118

Query: 498 CEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNI 662
           CEIETDKATLEFE LEEGFLAKIL  EGSKDVPVGQPIAITVED +DI  VP+++
Sbjct: 119 CEIETDKATLEFESLEEGFLAKILVAEGSKDVPVGQPIAITVEDEEDIQKVPASV 173



 Score =  160 bits (405), Expect = 2e-37
 Identities = 91/163 (55%), Positives = 113/163 (69%), Gaps = 9/163 (5%)
 Frame = +3

Query: 204 LSSITVA--SRYLASSSGLKISSIDVDETLLRPTALSGYTHISEKSSSI-------KLQI 356
           L+ I VA  S+ +     + I+  D ++    P +++G + + EK S         K Q 
Sbjct: 138 LAKILVAEGSKDVPVGQPIAITVEDEEDIQKVPASVAGGSGVEEKKSKHENAGNEDKQQE 197

Query: 357 GLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEVGDVICEIETDKATLEFE 536
                ++ E P HIV+ MPALSPTM QGNIAKW+KKEGDKIEVGDVICEIETDKATLEFE
Sbjct: 198 MSSTINTAELPPHIVLGMPALSPTMNQGNIAKWRKKEGDKIEVGDVICEIETDKATLEFE 257

Query: 537 CLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNIT 665
            LEEG+LAKI+APEGSKDV VGQPIAITVED DDI  V ++++
Sbjct: 258 SLEEGYLAKIVAPEGSKDVAVGQPIAITVEDPDDIEIVKASVS 300


>ref|XP_002526756.1| dihydrolipoamide acetyltransferase component of pyruvate
           dehydrogenase, putative [Ricinus communis]
           gi|223533883|gb|EEF35610.1| dihydrolipoamide
           acetyltransferase component of pyruvate dehydrogenase,
           putative [Ricinus communis]
          Length = 633

 Score =  214 bits (544), Expect = 2e-53
 Identities = 116/174 (66%), Positives = 138/174 (79%), Gaps = 2/174 (1%)
 Frame = +3

Query: 138 MALSRLRHPV-ICRAPSLF-KSRSLSSITVASRYLASSSGLKISSIDVDETLLRPTALSG 311
           MA SRLRH + I RAPSL  K+R LS+ + +    A  SG K S +D ++  LRPT++  
Sbjct: 1   MAFSRLRHQMMISRAPSLLLKTRVLSTSSRSVTRCAICSGAKHSFVDGNDFYLRPTSIFM 60

Query: 312 YTHISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEVGD 491
            T + +K   +KL IG+RHFSS+EP SH+VI MPALSPTMTQGN+AKW+KKEGDK++VGD
Sbjct: 61  ITGVHDKFLKLKLGIGVRHFSSSEP-SHMVIGMPALSPTMTQGNVAKWRKKEGDKVKVGD 119

Query: 492 VICEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVP 653
           V+CEIETDKATLEFE LEEGFLAKIL PEGSKDVPVGQPIAITVE+ DDI NVP
Sbjct: 120 VLCEIETDKATLEFESLEEGFLAKILTPEGSKDVPVGQPIAITVENEDDIQNVP 173



 Score =  162 bits (409), Expect = 7e-38
 Identities = 77/98 (78%), Positives = 89/98 (90%)
 Frame = +3

Query: 372 SSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEVGDVICEIETDKATLEFECLEEG 551
           +++E P H+ +EMPALSPTM QGNIAKW+KKEGDKIEVGDVICEIETDKATLEFECLEEG
Sbjct: 204 NTSELPPHVFLEMPALSPTMNQGNIAKWRKKEGDKIEVGDVICEIETDKATLEFECLEEG 263

Query: 552 FLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNIT 665
           +LAKILAPEGSKDV VGQPIA+TVED +DI  V ++I+
Sbjct: 264 YLAKILAPEGSKDVAVGQPIALTVEDPNDIETVKTSIS 301


>ref|XP_004138681.1| PREDICTED: dihydrolipoyllysine-residue acetyltransferase component
           1 of pyruvate dehydrogenase complex, mitochondrial-like
           [Cucumis sativus]
          Length = 638

 Score =  195 bits (496), Expect = 6e-48
 Identities = 108/178 (60%), Positives = 129/178 (72%), Gaps = 4/178 (2%)
 Frame = +3

Query: 138 MALSRLRHPVICRAPSLFKSRS---LSSITVASRYLASSSGLKISSIDV-DETLLRPTAL 305
           M+L RLR PVI RA SL  +R     SS  V+SRY++  S   +    V D +L RP   
Sbjct: 1   MSLHRLRDPVIVRARSLLHARLGAFHSSSPVSSRYISRYSTWNVQRFSVGDGSLFRPVPF 60

Query: 306 SGYTHISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEV 485
           S +T    ++  ++  +G+R FSST+  SH V+EMPALSPTM QGNIAKW+KKEGDK+ V
Sbjct: 61  SCFTGACGRALHLEQSVGIRFFSSTDS-SHAVLEMPALSPTMNQGNIAKWRKKEGDKVTV 119

Query: 486 GDVICEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSN 659
           GDV+CEIETDKATLEFE LEEG+LAKIL PEGSKDVPVGQPIAITVED DDI  V +N
Sbjct: 120 GDVLCEIETDKATLEFESLEEGYLAKILVPEGSKDVPVGQPIAITVEDPDDINRVLAN 177



 Score =  162 bits (410), Expect = 5e-38
 Identities = 81/121 (66%), Positives = 96/121 (79%)
 Frame = +3

Query: 303 LSGYTHISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIE 482
           +SG T + ++ S    Q      +S++ P HIV+EMPALSPTM QGNIA W+KKEGDKIE
Sbjct: 179 VSGATDVKQEKSEASAQASSVEINSSKLPPHIVLEMPALSPTMNQGNIATWRKKEGDKIE 238

Query: 483 VGDVICEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNI 662
           VGDVICEIETDKATLEFE LEEG+LAKILAPEGSKDV VG+PIAITVED  DI +V + +
Sbjct: 239 VGDVICEIETDKATLEFESLEEGYLAKILAPEGSKDVAVGKPIAITVEDLADIESVKNAV 298

Query: 663 T 665
           +
Sbjct: 299 S 299


>ref|XP_004159237.1| PREDICTED: dihydrolipoyllysine-residue acetyltransferase component
           1 of pyruvate dehydrogenase complex, mitochondrial-like
           [Cucumis sativus]
          Length = 638

 Score =  195 bits (495), Expect = 7e-48
 Identities = 107/178 (60%), Positives = 129/178 (72%), Gaps = 4/178 (2%)
 Frame = +3

Query: 138 MALSRLRHPVICRAPSLFKSRS---LSSITVASRYLASSSGLKISSIDV-DETLLRPTAL 305
           M+L RLR PVI RA SL  +R     SS  ++SRY++  S   +    V D +L RP   
Sbjct: 1   MSLHRLRDPVIVRARSLLHARLGAFHSSSPISSRYISRYSTWNVQRFSVGDGSLFRPVPF 60

Query: 306 SGYTHISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIEV 485
           S +T    ++  ++  +G+R FSST+  SH V+EMPALSPTM QGNIAKW+KKEGDK+ V
Sbjct: 61  SCFTGACGRALHLEQSVGIRFFSSTDS-SHAVLEMPALSPTMNQGNIAKWRKKEGDKVTV 119

Query: 486 GDVICEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSN 659
           GDV+CEIETDKATLEFE LEEG+LAKIL PEGSKDVPVGQPIAITVED DDI  V +N
Sbjct: 120 GDVLCEIETDKATLEFESLEEGYLAKILVPEGSKDVPVGQPIAITVEDPDDINRVLAN 177



 Score =  163 bits (413), Expect = 2e-38
 Identities = 82/121 (67%), Positives = 96/121 (79%)
 Frame = +3

Query: 303 LSGYTHISEKSSSIKLQIGLRHFSSTEPPSHIVIEMPALSPTMTQGNIAKWKKKEGDKIE 482
           +SG T + ++ S    Q      +S++ P HIV+EMPALSPTM QGNIA W+KKEGDKIE
Sbjct: 179 VSGATDVKQEKSEASAQASSVEINSSKLPPHIVLEMPALSPTMNQGNIATWRKKEGDKIE 238

Query: 483 VGDVICEIETDKATLEFECLEEGFLAKILAPEGSKDVPVGQPIAITVEDADDIPNVPSNI 662
           VGDVICEIETDKATLEFE LEEG+LAKILAPEGSKDV VG+PIAITVED  DI +V S +
Sbjct: 239 VGDVICEIETDKATLEFESLEEGYLAKILAPEGSKDVAVGKPIAITVEDPADIESVKSAV 298

Query: 663 T 665
           +
Sbjct: 299 S 299