BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00029829
         (1203 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273941.2| PREDICTED: putative E3 ubiquitin-protein lig...   401   e-109
emb|CBI30594.3| unnamed protein product [Vitis vinifera]              401   e-109
ref|XP_002305782.1| predicted protein [Populus trichocarpa] gi|2...   360   4e-97
emb|CBI21880.3| unnamed protein product [Vitis vinifera]              357   3e-96
ref|XP_002329795.1| predicted protein [Populus trichocarpa] gi|2...   350   4e-94

>ref|XP_002273941.2| PREDICTED: putative E3 ubiquitin-protein ligase RING1a-like [Vitis
            vinifera]
          Length = 534

 Score =  401 bits (1030), Expect = e-109
 Identities = 219/403 (54%), Positives = 267/403 (66%), Gaps = 4/403 (0%)
 Frame = +2

Query: 5    SPASSIGDEDEYITVTLPDLRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 184
            SP+ S G++DE++ V L ++RKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 139  SPSWSNGEKDEFVIVKLAEIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 198

Query: 185  ECPACRTHCASRRSLRDDPNFDTLISVLYPDIDKYXXXXXXXXXXXRTRFKQIQASIAQT 364
            ECPACRTHCASRRSLRDDPN+D LI+ LYPDIDKY           +TR KQIQASIAQT
Sbjct: 199  ECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEQEELAFHEEEKTRNKQIQASIAQT 258

Query: 365  LHRQSEALARKRPSGK-XXXXXXXXXXXXXXXXXXNQRAVEQQGSDEQDGANGHDRAKDS 541
            L RQSEAL RKR + K                   N RA +  GS++ + ANG+D  KDS
Sbjct: 259  LRRQSEALGRKRTTAKATAAAFARRSQGNYRRGRRNYRATDLLGSEDDEDANGNDGGKDS 318

Query: 542  SSADEPVTEVQPKRCKRWTASRFSQTS--LXXXXXXXXXXXXXXNRDSLGASAGLVAHSE 715
            SSA++   EV+PKR +RW   RFSQ S                 NR+++GASAGLV +SE
Sbjct: 319  SSAEDHSAEVKPKRSRRWGGVRFSQPSSGAANADGGGDENDLEPNREAMGASAGLVGNSE 378

Query: 716  LLTWGSGGIRSNTRHSSLSAGNAKNARNMRMSKLFDSFQNTVKVDG-MDIHLILVPLDEQ 892
            +L WG GG+RS+TR+ S+S GN KNAR+ R+SKL D  +N  + D  +DIHL+LV LDEQ
Sbjct: 379  ILAWGKGGMRSHTRYGSVSGGNGKNARSSRLSKLVDYLRNLDQNDDELDIHLMLVSLDEQ 438

Query: 893  RIPSLENPHIRCSPTVSVRYLCQHVGSKTTVSADEVEILLVKGLHSIPNLTVNPSTFVPE 1072
            RIPSL+ P++ C PT+SVR+LCQ+V  +T + ADEVEIL++K   S     +NPS     
Sbjct: 439  RIPSLQTPYLCCRPTLSVRHLCQYVALQTPLLADEVEILVIKEQLS----KLNPSN---S 491

Query: 1073 RSIDPCRDXXXXXXXXXXXXXXXXXYNVNRRSLLFAYRQKLSA 1201
             S DPC D                  N N+  LL AYR+KLSA
Sbjct: 492  SSPDPCSDRLQVLEAQETLAVLKTMRNANQSHLLLAYRRKLSA 534


>emb|CBI30594.3| unnamed protein product [Vitis vinifera]
          Length = 433

 Score =  401 bits (1030), Expect = e-109
 Identities = 219/403 (54%), Positives = 267/403 (66%), Gaps = 4/403 (0%)
 Frame = +2

Query: 5    SPASSIGDEDEYITVTLPDLRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 184
            SP+ S G++DE++ V L ++RKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 38   SPSWSNGEKDEFVIVKLAEIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 97

Query: 185  ECPACRTHCASRRSLRDDPNFDTLISVLYPDIDKYXXXXXXXXXXXRTRFKQIQASIAQT 364
            ECPACRTHCASRRSLRDDPN+D LI+ LYPDIDKY           +TR KQIQASIAQT
Sbjct: 98   ECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEQEELAFHEEEKTRNKQIQASIAQT 157

Query: 365  LHRQSEALARKRPSGK-XXXXXXXXXXXXXXXXXXNQRAVEQQGSDEQDGANGHDRAKDS 541
            L RQSEAL RKR + K                   N RA +  GS++ + ANG+D  KDS
Sbjct: 158  LRRQSEALGRKRTTAKATAAAFARRSQGNYRRGRRNYRATDLLGSEDDEDANGNDGGKDS 217

Query: 542  SSADEPVTEVQPKRCKRWTASRFSQTS--LXXXXXXXXXXXXXXNRDSLGASAGLVAHSE 715
            SSA++   EV+PKR +RW   RFSQ S                 NR+++GASAGLV +SE
Sbjct: 218  SSAEDHSAEVKPKRSRRWGGVRFSQPSSGAANADGGGDENDLEPNREAMGASAGLVGNSE 277

Query: 716  LLTWGSGGIRSNTRHSSLSAGNAKNARNMRMSKLFDSFQNTVKVDG-MDIHLILVPLDEQ 892
            +L WG GG+RS+TR+ S+S GN KNAR+ R+SKL D  +N  + D  +DIHL+LV LDEQ
Sbjct: 278  ILAWGKGGMRSHTRYGSVSGGNGKNARSSRLSKLVDYLRNLDQNDDELDIHLMLVSLDEQ 337

Query: 893  RIPSLENPHIRCSPTVSVRYLCQHVGSKTTVSADEVEILLVKGLHSIPNLTVNPSTFVPE 1072
            RIPSL+ P++ C PT+SVR+LCQ+V  +T + ADEVEIL++K   S     +NPS     
Sbjct: 338  RIPSLQTPYLCCRPTLSVRHLCQYVALQTPLLADEVEILVIKEQLS----KLNPSN---S 390

Query: 1073 RSIDPCRDXXXXXXXXXXXXXXXXXYNVNRRSLLFAYRQKLSA 1201
             S DPC D                  N N+  LL AYR+KLSA
Sbjct: 391  SSPDPCSDRLQVLEAQETLAVLKTMRNANQSHLLLAYRRKLSA 433


>ref|XP_002305782.1| predicted protein [Populus trichocarpa] gi|222848746|gb|EEE86293.1|
            predicted protein [Populus trichocarpa]
          Length = 463

 Score =  360 bits (924), Expect = 4e-97
 Identities = 192/371 (51%), Positives = 248/371 (66%), Gaps = 9/371 (2%)
 Frame = +2

Query: 11   ASSIGDE--DEYITVTLPDLRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 184
            +SSI ++  +EY+ V L ++RKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 65   SSSINEDVKEEYVVVKLSEIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 124

Query: 185  ECPACRTHCASRRSLRDDPNFDTLISVLYPDIDKYXXXXXXXXXXXRTRFKQIQASIAQT 364
            ECPACRTHCASRRSLRDDPN+D LIS LYPDIDKY           + R KQIQA+IAQT
Sbjct: 125  ECPACRTHCASRRSLRDDPNYDALISALYPDIDKYEEEELAFQEEEKARNKQIQATIAQT 184

Query: 365  LHRQSEALARKRPSGK-----XXXXXXXXXXXXXXXXXXNQRAVEQQGSDEQDGANGHDR 529
             HRQ+EAL+RK+ + K                       N R    QGSD+ + ANG D 
Sbjct: 185  FHRQAEALSRKKTTAKVTAAAFARRTPSRFRDAHSRGRRNYRMAGFQGSDDNEDANG-DG 243

Query: 530  AKDSSSADEPVTEVQPKRCKRWTASRFSQTSLXXXXXXXXXXXXXXNRDSLGASAGLVAH 709
             KDSSSADE   EV+PKRCK+W  +R   ++               N++S+G S+GLV  
Sbjct: 244  GKDSSSADEHSAEVKPKRCKKWAGAR---SAAVNADGGGDENDSEVNKESVGGSSGLVGS 300

Query: 710  SELLTWGSGGIRSNTRHSSLSAGNAKNARNMRMSKLFDSFQNTVKVDG-MDIHLILVPLD 886
            SE L WG  G+RS+TR+ S +  N KNAR+ R+SKL D  +N    D  +DI+L+LV  D
Sbjct: 301  SERLAWGKNGMRSHTRYGSANGSNVKNARSSRISKLADYLRNLEGNDNELDINLMLVSFD 360

Query: 887  EQRIPSLENPHIRCSPTVSVRYLCQHVGSKTTVSADEVEILLVKGLHSIPNLTVNPSTFV 1066
            EQR+PSL+ P++ C PT+S+  LCQ+V  +T++ A+EVEI LV+ ++S  + +++ ST +
Sbjct: 361  EQRVPSLQRPYLCCRPTLSINKLCQYVAFQTSLQANEVEIYLVREMNSKVDFSISMSTPI 420

Query: 1067 PERS-IDPCRD 1096
             +   IDPC+D
Sbjct: 421  SKPGIIDPCKD 431


>emb|CBI21880.3| unnamed protein product [Vitis vinifera]
          Length = 568

 Score =  357 bits (916), Expect = 3e-96
 Identities = 201/411 (48%), Positives = 254/411 (61%), Gaps = 14/411 (3%)
 Frame = +2

Query: 2    ESPASSIGDEDEYITVTLPDLRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGN 181
            E+P+SS  ++ E++ V LPD+RK+VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN
Sbjct: 155  ENPSSSTEEKPEFVFVGLPDIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGN 214

Query: 182  NECPACRTHCASRRSLRDDPNFDTLISVLYPDIDKYXXXXXXXXXXXRTRFKQIQASIAQ 361
            NECPACRTHCASRRSLRDDPN+D LI+ LYPDIDKY           +TR KQIQASIAQ
Sbjct: 215  NECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELAFHEEEQTRNKQIQASIAQ 274

Query: 362  TLHRQSEALARKRPSGK-----XXXXXXXXXXXXXXXXXXNQRAVEQQGSDEQDGANGHD 526
             L RQSEAL RKR  GK                       N R  + QGSD+ +  N +D
Sbjct: 275  ILQRQSEALNRKRTLGKDIAGPFMTRSQRNYRNAYSRRRKNGRGSDLQGSDDNEDENDND 334

Query: 527  RAKDSSSADEPVTEVQPKRCKRWTASRF---SQTSLXXXXXXXXXXXXXXNRDSLGASAG 697
              K SSSA+E  TEV+ +R KR    R    S +S+              NR+S G S G
Sbjct: 335  GGKGSSSAEERSTEVKRRRYKRRAGIRLIQPSSSSVANSDGGCIENDLEVNRESRGFSPG 394

Query: 698  LVAHSELLTWGSGGIRSNTRHSSLSAGNAKNARNMRMSKLFDSFQNTVKVDG-MDIHLIL 874
            LV + E+L WG GG RS+TRH S S  N K+AR+ R+SKL D  ++  + D  +D+HL+L
Sbjct: 395  LVWNPEMLAWGRGGARSHTRHGSSSGCNHKSARSTRLSKLVDYLRSLGENDNELDVHLML 454

Query: 875  VPLDEQRIPSLENPHIRCSPTVSVRYLCQHVGSKTTVSADEVEILLVKGLHSIPNLTVNP 1054
            + LD++  PSL+ P++ C PT+SVR+LC++V  +T + A+EVEIL+VKG H+      NP
Sbjct: 455  ISLDKESTPSLQEPYLCCRPTLSVRHLCEYVALQTPLRAEEVEILVVKGCHNDYQPFPNP 514

Query: 1055 STFVPERS-----IDPCRDXXXXXXXXXXXXXXXXXYNVNRRSLLFAYRQK 1192
            ST V + +     IDP +D                     R  L+ AYRQK
Sbjct: 515  STSVDDLNPDPIVIDPSKDELQILEGQETLVGLQANGTSCRNQLVLAYRQK 565


>ref|XP_002329795.1| predicted protein [Populus trichocarpa] gi|222870857|gb|EEF07988.1|
            predicted protein [Populus trichocarpa]
          Length = 445

 Score =  350 bits (898), Expect = 4e-94
 Identities = 190/372 (51%), Positives = 249/372 (66%), Gaps = 9/372 (2%)
 Frame = +2

Query: 8    PASSIGDE--DEYITVTLPDLRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGN 181
            P+S+I ++  +EY+ V L ++RKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGN
Sbjct: 53   PSSNINEDVKEEYVVVKLSEIRKEVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGN 112

Query: 182  NECPACRTHCASRRSLRDDPNFDTLISVLYPDIDKYXXXXXXXXXXXRTRFKQIQASIAQ 361
            NECPACRTHCASRRSLRDDPN+D LI+ LYPDIDKY           + R K+IQA+IAQ
Sbjct: 113  NECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELAFQEDEKARNKEIQATIAQ 172

Query: 362  TLHRQSEALARKRPSGK-----XXXXXXXXXXXXXXXXXXNQRAVEQQGSDEQDGANGHD 526
            T  RQ+EAL+RKR + K                       N R  E QGSD+ + ANG  
Sbjct: 173  TFDRQAEALSRKRSTAKATAAVFARRTPSRFRDAHSRGRRNYRIAELQGSDDNEDANG-G 231

Query: 527  RAKDSSSADEPVTEVQPKRCKRWTASRFSQTSLXXXXXXXXXXXXXXNRDSLGASAGLVA 706
              KDSSS DE   EV+PKR +R +A+  +  S               N++S+GASAGL++
Sbjct: 232  GGKDSSSTDEHSAEVKPKRYRRCSAAFNADGS-------GGENDSEVNKESVGASAGLIS 284

Query: 707  HSELLTWGSGGIRSNTRHSSLSAGNAKNARNMRMSKLFDSFQNTVKVDG-MDIHLILVPL 883
             SE L WG  G+RS+TR+ S +  N KNARN R+SKL D  +N  + D  +DI+L+LV  
Sbjct: 285  SSERLAWGKNGMRSHTRYGSANGSNVKNARNSRISKLADYLRNLDENDNELDINLMLVSF 344

Query: 884  DEQRIPSLENPHIRCSPTVSVRYLCQHVGSKTTVSADEVEILLVKGLHSIPNLTVNPSTF 1063
            DEQR+PSL+ P++ C PT+S++ LCQ+V  +T++ A+EVEI LV+ ++S  + +++ S+ 
Sbjct: 345  DEQRVPSLQRPYLCCRPTLSIKSLCQYVAFQTSLQANEVEIYLVQDMNSKLDFSLSMSSP 404

Query: 1064 VPERS-IDPCRD 1096
            V     IDPC+D
Sbjct: 405  VSRHGIIDPCKD 416