BLASTX nr result
ID: Bupleurum21_contig00029356
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00029356
(535 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative rib... 109 2e-34
emb|CAN76892.1| hypothetical protein VITISV_005356 [Vitis vinifera] 106 1e-31
gb|ABN08038.1| RNA-directed DNA polymerase (Reverse transcriptas... 113 5e-31
dbj|BAM28751.1| reverse transcriptase-like protein, partial [Cam... 101 9e-31
emb|CAN77449.1| hypothetical protein VITISV_016970 [Vitis vinifera] 115 2e-30
>ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative ribonuclease H protein
At1g65750-like [Vitis vinifera]
Length = 820
Score = 109 bits (272), Expect(3) = 2e-34
Identities = 46/90 (51%), Positives = 63/90 (70%)
Frame = -2
Query: 534 KSLSREGKDLLLKMVAQALPNYAMSTFLLPQQICADMEKIMNQFWWKGSKTSKGIHWMSW 355
+ LSR GK++ LK VAQ++P Y M LLP+ +C D+E +MN F+W + + I WMSW
Sbjct: 250 RRLSRAGKEICLKTVAQSIPTYVMQLLLLPKDLCRDIESMMNGFFWDSNPQCRSIRWMSW 309
Query: 354 DLMCEKKSIGGLGFRNLQEYNMALLGKQGW 265
MC++K GGLGFR L ++N+ALL KQGW
Sbjct: 310 GKMCKQKKDGGLGFRKLXDFNLALLAKQGW 339
Score = 57.0 bits (136), Expect(3) = 2e-34
Identities = 24/42 (57%), Positives = 35/42 (83%)
Frame = -3
Query: 263 NPNSLVGKVYKARYYPDGSLLTAKIGSNPSYIWRSIMEAQVL 138
NP+SLV ++++ARY+ + S L A++GSNPSY+WRSI+ AQ L
Sbjct: 344 NPDSLVTRIFQARYFRNSSFLNAELGSNPSYMWRSILAAQGL 385
Score = 25.0 bits (53), Expect(3) = 2e-34
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 124 VGKGTTINILEDPWLPDSN 68
+ GT + + D WLPDS+
Sbjct: 394 IASGTKVQVWGDSWLPDSS 412
>emb|CAN76892.1| hypothetical protein VITISV_005356 [Vitis vinifera]
Length = 1793
Score = 106 bits (265), Expect(3) = 1e-31
Identities = 45/89 (50%), Positives = 63/89 (70%)
Frame = -2
Query: 534 KSLSREGKDLLLKMVAQALPNYAMSTFLLPQQICADMEKIMNQFWWKGSKTSKGIHWMSW 355
+ LSR GK++ LK VAQ++P Y M LLP+ +C D+E +MN F+W + + I WMSW
Sbjct: 1337 RRLSRAGKEICLKTVAQSIPTYVMQLLLLPKDLCRDIESMMNXFFWDSNPQXRSIRWMSW 1396
Query: 354 DLMCEKKSIGGLGFRNLQEYNMALLGKQG 268
MC++K GGLGFR L+++N+ALL KQG
Sbjct: 1397 XKMCKQKKDGGLGFRKLEDFNLALLAKQG 1425
Score = 51.2 bits (121), Expect(3) = 1e-31
Identities = 23/42 (54%), Positives = 33/42 (78%)
Frame = -3
Query: 263 NPNSLVGKVYKARYYPDGSLLTAKIGSNPSYIWRSIMEAQVL 138
N +SLV +++ARY+ + S L A++GSNPSY+WRSI+ AQ L
Sbjct: 1431 NLDSLVTIIFQARYFRNSSFLNAELGSNPSYMWRSILAAQDL 1472
Score = 24.3 bits (51), Expect(3) = 1e-31
Identities = 11/29 (37%), Positives = 16/29 (55%), Gaps = 1/29 (3%)
Frame = -1
Query: 124 VGKGTTINILEDPWLPD-SNPYVHTNHEA 41
+ GT + + D WLP+ SN + T H A
Sbjct: 1481 IASGTKVQVWGDSWLPNSSNRLIITPHVA 1509
>gb|ABN08038.1| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
truncatula]
Length = 573
Score = 113 bits (283), Expect(3) = 5e-31
Identities = 47/91 (51%), Positives = 69/91 (75%), Gaps = 1/91 (1%)
Frame = -2
Query: 534 KSLSREGKDLLLKMVAQALPNYAMSTFLLPQQICADMEKIMNQFWWKGSKTSK-GIHWMS 358
+SLSR K++L+K VAQA+P+Y M FL+P +C ++E++MN F+W K + GI+WM
Sbjct: 313 RSLSRADKEVLIKSVAQAIPSYCMGAFLIPASLCEELERMMNSFYWGSKKNGRRGINWMR 372
Query: 357 WDLMCEKKSIGGLGFRNLQEYNMALLGKQGW 265
WD + KS+GGLGFRNL+ +N+++LGKQGW
Sbjct: 373 WDFLTLHKSLGGLGFRNLEAFNLSMLGKQGW 403
Score = 42.4 bits (98), Expect(3) = 5e-31
Identities = 18/40 (45%), Positives = 25/40 (62%)
Frame = -3
Query: 257 NSLVGKVYKARYYPDGSLLTAKIGSNPSYIWRSIMEAQVL 138
+SL ++ KA+Y+P L A +G NPSY WRS+ Q L
Sbjct: 410 SSLFTRILKAKYFPRRDFLDATLGHNPSYTWRSLWSTQSL 449
Score = 23.9 bits (50), Expect(3) = 5e-31
Identities = 12/48 (25%), Positives = 23/48 (47%), Gaps = 7/48 (14%)
Frame = -1
Query: 127 RVGKGTTINILEDPWL-------PDSNPYVHTNHEAVQHKTVDVLMNS 5
++G G+ IN+ PW+ P ++P +H H+ + + NS
Sbjct: 457 KIGDGSKINVWSMPWIRNLLSLKPSTSPPLH-QHDLTVNSLLSADFNS 503
>dbj|BAM28751.1| reverse transcriptase-like protein, partial [Camellia sinensis var.
sinensis]
Length = 255
Score = 101 bits (251), Expect(3) = 9e-31
Identities = 44/90 (48%), Positives = 61/90 (67%)
Frame = -2
Query: 534 KSLSREGKDLLLKMVAQALPNYAMSTFLLPQQICADMEKIMNQFWWKGSKTSKGIHWMSW 355
KSLS G+++L+K VAQA+P Y MS F LP +C ++E ++ FWW + ++ IHW SW
Sbjct: 31 KSLSVAGREVLIKSVAQAIPTYIMSCFRLPDGVCXEIESMVCNFWWGQKEETRKIHWTSW 90
Query: 354 DLMCEKKSIGGLGFRNLQEYNMALLGKQGW 265
+C+ K GG+GFR LQ +N ALL KQ W
Sbjct: 91 KQLCKSKLRGGMGFRCLQHFNSALLAKQCW 120
Score = 53.9 bits (128), Expect(3) = 9e-31
Identities = 24/44 (54%), Positives = 33/44 (75%)
Frame = -3
Query: 260 PNSLVGKVYKARYYPDGSLLTAKIGSNPSYIWRSIMEAQVLCSA 129
P SLV +V+KARY+P S L A +GSNPS+ WRSI+ ++ L +A
Sbjct: 126 PQSLVARVFKARYFPQTSFLDALVGSNPSFTWRSIVGSRRLLNA 169
Score = 23.9 bits (50), Expect(3) = 9e-31
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = -1
Query: 127 RVGKGTTINILEDPWLP 77
RVG G +I++ D W+P
Sbjct: 174 RVGNGASISVKYDKWVP 190
>emb|CAN77449.1| hypothetical protein VITISV_016970 [Vitis vinifera]
Length = 517
Score = 115 bits (287), Expect(2) = 2e-30
Identities = 51/92 (55%), Positives = 72/92 (78%), Gaps = 2/92 (2%)
Frame = -2
Query: 534 KSLSREGKDLLLKMVAQALPNYAMSTFLLPQQICADMEKIMNQFWWKGSKTSKG--IHWM 361
K LS+ GK +L+K+V QA+P+Y MSTFLLP + +++K+MN FWW G K+ G I+W+
Sbjct: 183 KLLSQAGKKILIKVVGQAIPSYCMSTFLLPISLSEELQKMMNSFWW-GPKSDGGRRINWL 241
Query: 360 SWDLMCEKKSIGGLGFRNLQEYNMALLGKQGW 265
SWD +C +K GGLGFRNL+++N+A+LGKQGW
Sbjct: 242 SWDKLCLRKEEGGLGFRNLRDFNLAMLGKQGW 273
Score = 42.7 bits (99), Expect(2) = 2e-30
Identities = 16/41 (39%), Positives = 27/41 (65%)
Frame = -3
Query: 260 PNSLVGKVYKARYYPDGSLLTAKIGSNPSYIWRSIMEAQVL 138
P+SL+ ++ KARYY L+A++ NPS+ W+ I +Q +
Sbjct: 279 PDSLIARLLKARYYCSRDFLSARLSHNPSFTWKGIWSSQAI 319