BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00029130
         (561 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI30150.3| unnamed protein product [Vitis vinifera]              158   6e-37
ref|XP_004143360.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...   124   8e-27
ref|XP_002326378.1| predicted protein [Populus trichocarpa] gi|2...   123   2e-26
ref|XP_002266033.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...   112   3e-23
ref|NP_195965.2| transducin/WD40 domain-containing protein [Arab...   109   3e-22

>emb|CBI30150.3| unnamed protein product [Vitis vinifera]
          Length = 610

 Score =  158 bits (399), Expect = 6e-37
 Identities = 90/194 (46%), Positives = 117/194 (60%), Gaps = 8/194 (4%)
 Frame = +2

Query: 2   SGGDHQVCCLPCGHIYGLXXXXXXXXXXXXXXXXXXXXXXXX-MKDVRLLYASRIVAVDE 178
           SGG+HQVCCLPCGHIYG+                         +++VR LYASR+VAVDE
Sbjct: 118 SGGEHQVCCLPCGHIYGISCIKKWFQQCGRSSGKCPQCKRKCTLRNVRTLYASRVVAVDE 177

Query: 179 ELRKKVQTLEAKCASLEMKRSECC-------KREAELHMQVHRLKERASSLEGMLEYSER 337
           +LRKK+++LEAKCASLE K ++ C       KREA+LH+QV +L ER +       Y ER
Sbjct: 178 DLRKKIRSLEAKCASLEKKNADWCMKESDWHKREADLHLQVQQLIERTT-------YLER 230

Query: 338 ILSGSITSSSGFLGRSTPGCKFDSAFMKTNSSCNFALQKEFDIEGARIFDVDAIGQTVVI 517
           + S      SG +  +  G  F   F +  SSCNF LQKE  ++GAR FD+DA G+ +VI
Sbjct: 231 LSSEMHGRGSGLV--AARGHNFAENFGRRGSSCNFVLQKELQVDGARYFDIDASGEILVI 288

Query: 518 ARRLAGMGALNALT 559
           ARRL+G G  + LT
Sbjct: 289 ARRLSGTGGTHVLT 302


>ref|XP_004143360.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3-like [Cucumis sativus]
           gi|449482586|ref|XP_004156335.1| PREDICTED: E3
           ubiquitin-protein ligase RFWD3-like [Cucumis sativus]
          Length = 617

 Score =  124 bits (312), Expect = 8e-27
 Identities = 74/193 (38%), Positives = 104/193 (53%), Gaps = 9/193 (4%)
 Frame = +2

Query: 8   GDHQVCCLPCGHIYGLXXXXXXXXXXXXXXXXXXXXXXXXMKDVRLLYASRIVAVDEELR 187
           G+H +CCLPCGH+YG+                         KDVR ++A RIVA D E +
Sbjct: 123 GEHHICCLPCGHLYGMSCINRWLQQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGESQ 182

Query: 188 KKVQTLEAKCASLEMKRSECC-------KREAELHMQVHRLKERASSLEGMLEYSER--I 340
           K++++LEA CASLE K +  C       KRE ++  ++  L ER + LE +LE S+R  +
Sbjct: 183 KRIKSLEATCASLEKKEANWCKKEVEWKKRETDMQSKLRELSERTNYLEHLLEDSQRKPV 242

Query: 341 LSGSITSSSGFLGRSTPGCKFDSAFMKTNSSCNFALQKEFDIEGARIFDVDAIGQTVVIA 520
           L+        F G+S     F   F    SSC+F L+KE  ++GAR+ DVDA  Q V++A
Sbjct: 243 LA---NLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLIDVDASNQIVLVA 299

Query: 521 RRLAGMGALNALT 559
           RR  G G  + LT
Sbjct: 300 RR--GFGGTHVLT 310


>ref|XP_002326378.1| predicted protein [Populus trichocarpa] gi|222833571|gb|EEE72048.1|
           predicted protein [Populus trichocarpa]
          Length = 681

 Score =  123 bits (308), Expect = 2e-26
 Identities = 72/191 (37%), Positives = 101/191 (52%), Gaps = 7/191 (3%)
 Frame = +2

Query: 8   GDHQVCCLPCGHIYGLXXXXXXXXXXXXXXXXXXXXXXXXMKDVRLLYASRIVAVDEELR 187
           GDH +CCLPCGH++GL                        ++D+R L+  RI  VDEE +
Sbjct: 185 GDHHICCLPCGHLFGLSCIEKWLRQRGRLAKCPQCNRKGTLRDIRKLFVPRIAVVDEESQ 244

Query: 188 KKVQTLEAKCASLEMKRSECCK-------REAELHMQVHRLKERASSLEGMLEYSERILS 346
           K+++ LE KCASL  K  + CK       REA L ++V + KER   LE +L   +   S
Sbjct: 245 KRIRFLEEKCASLVKKDVDLCKKESEWKRREAALQLEVQKCKERTIYLEHLLGDLQSRPS 304

Query: 347 GSITSSSGFLGRSTPGCKFDSAFMKTNSSCNFALQKEFDIEGARIFDVDAIGQTVVIARR 526
           G  T+  G  G    G    S F +  SS  F LQ+E  ++GAR+FDVDA G+ +++ RR
Sbjct: 305 GVATAYRGSQGHEF-GNSIYSDFNRQGSSSCFMLQRELRVDGARLFDVDASGKILLLVRR 363

Query: 527 LAGMGALNALT 559
           +  MG  + LT
Sbjct: 364 VPMMGGSHVLT 374


>ref|XP_002266033.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3-like [Vitis vinifera]
          Length = 567

 Score =  112 bits (281), Expect = 3e-23
 Identities = 71/194 (36%), Positives = 94/194 (48%), Gaps = 8/194 (4%)
 Frame = +2

Query: 2   SGGDHQVCCLPCGHIYGLXXXXXXXXXXXXXXXXXXXXXXXX-MKDVRLLYASRIVAVDE 178
           SGG+HQVCCLPCGHIYG+                         +++VR LYASR+VAVDE
Sbjct: 118 SGGEHQVCCLPCGHIYGISCIKKWFQQCGRSSGKCPQCKRKCTLRNVRTLYASRVVAVDE 177

Query: 179 ELRKKVQTLEAKCASLEMKRSECC-------KREAELHMQVHRLKERASSLEGMLEYSER 337
           +LRKK+++LEAKCASLE K ++ C       KREA+LH+QV +L E+   ++G       
Sbjct: 178 DLRKKIRSLEAKCASLEKKNADWCMKESDWHKREADLHLQVQQLIEKELQVDG------- 230

Query: 338 ILSGSITSSSGFLGRSTPGCKFDSAFMKTNSSCNFALQKEFDIEGARIFDVDAIGQTVVI 517
                                                        AR FD+DA G+ +VI
Sbjct: 231 ---------------------------------------------ARYFDIDASGEILVI 245

Query: 518 ARRLAGMGALNALT 559
           ARRL+G G  + LT
Sbjct: 246 ARRLSGTGGTHVLT 259


>ref|NP_195965.2| transducin/WD40 domain-containing protein [Arabidopsis thaliana]
           gi|7378628|emb|CAB83304.1| putative protein [Arabidopsis
           thaliana] gi|209529765|gb|ACI49777.1| At5g03450
           [Arabidopsis thaliana] gi|332003223|gb|AED90606.1|
           transducin/WD40 domain-containing protein [Arabidopsis
           thaliana]
          Length = 630

 Score =  109 bits (273), Expect = 3e-22
 Identities = 65/193 (33%), Positives = 98/193 (50%), Gaps = 7/193 (3%)
 Frame = +2

Query: 2   SGGDHQVCCLPCGHIYGLXXXXXXXXXXXXXXXXXXXXXXXXMKDVRLLYASRIVAVDEE 181
           SGG HQVCCLPCGH+YG                         ++DVR +YASR+ AVD+E
Sbjct: 129 SGGQHQVCCLPCGHLYGYSCINKWFQQRRSGGKCPLCNKICSLRDVRKIYASRVAAVDDE 188

Query: 182 LRKKVQTLEAKCASLEMKRSECCKR-------EAELHMQVHRLKERASSLEGMLEYSERI 340
             K++  LEAK  S+E K +    +       EAEL ++V +LK++ +++E   + S+  
Sbjct: 189 AHKRILFLEAKLRSIEQKTASWSNKEVQWKITEAELRLEVTKLKKKIANMESTTKGSQS- 247

Query: 341 LSGSITSSSGFLGRSTPGCKFDSAFMKTNSSCNFALQKEFDIEGARIFDVDAIGQTVVIA 520
              ++ S   ++    P           + SC+F  Q +  I G RIFD+D   Q ++ A
Sbjct: 248 -QSNVASQEKYI----PAHNIYQEHHGQSPSCSFRHQGDLLINGGRIFDIDGGRQILLYA 302

Query: 521 RRLAGMGALNALT 559
           RRL+G+G    LT
Sbjct: 303 RRLSGVGGTFVLT 315