BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00028648
         (747 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265458.1| PREDICTED: diphthamide biosynthesis protein ...   384   e-105
ref|XP_003610873.1| Diphthamide biosynthesis protein [Medicago t...   384   e-104
ref|XP_002529006.1| Diphthamide biosynthesis protein, putative [...   376   e-102
ref|XP_003540663.1| PREDICTED: diphthamide biosynthesis protein ...   371   e-100
ref|XP_004136856.1| PREDICTED: diphthamide biosynthesis protein ...   369   e-100

>ref|XP_002265458.1| PREDICTED: diphthamide biosynthesis protein 1-like [Vitis vinifera]
          Length = 461

 Score =  384 bits (987), Expect = e-105
 Identities = 186/247 (75%), Positives = 213/247 (86%)
 Frame = +3

Query: 6   IPCLYVFVDIKIDVNKLVGTIKLNLSDKDINKIVLAGTIQFGTAIRVAKPELEKLGFKVL 185
           IPCLYVFV+I IDV+ L+GT+KLNL      +IVLAGTIQF +AIR AKPELEK GF+VL
Sbjct: 148 IPCLYVFVEIAIDVDLLIGTVKLNLPPA--GQIVLAGTIQFSSAIRAAKPELEKAGFRVL 205

Query: 186 VPQSKPLSAGEVLGCTAPTIMGKQFDEEGEIVLVFVADGRFHLEAFMIANPGIQAYRYDP 365
           +PQSKPLSAGEVLGCTAP I    F  + ++V VFVADGRFHLEAFMIANP I+AYRYDP
Sbjct: 206 IPQSKPLSAGEVLGCTAPKISKTSFISD-DVVAVFVADGRFHLEAFMIANPAIKAYRYDP 264

Query: 366 YIGKLFLEEYDHKGMKECRQNAIMKAREAKHWGVVLGTLGRQGNPRILDRLQKKMMEKDL 545
           YIG+L LEEYDHKGMK+ R+NAI+KARE K+WG+VLGTLGRQGNPRIL+ LQ +M EK  
Sbjct: 265 YIGRLLLEEYDHKGMKDSRKNAILKAREGKNWGIVLGTLGRQGNPRILNMLQDRMREKGF 324

Query: 546 DWTVVLMSELSPARIALFGDSVDAWVQIACPRLSIDWGDAFVKPLLNPFEAEIALGDLPG 725
            WTVVLMSE+SPAR+ LFGDSVDAW+QIACPRLSIDWGDAF KPLLNPFEAEIALG +PG
Sbjct: 325 SWTVVLMSEISPARVLLFGDSVDAWIQIACPRLSIDWGDAFGKPLLNPFEAEIALGFIPG 384

Query: 726 WWERSGV 746
           WWE++ +
Sbjct: 385 WWEKASI 391


>ref|XP_003610873.1| Diphthamide biosynthesis protein [Medicago truncatula]
           gi|355512208|gb|AES93831.1| Diphthamide biosynthesis
           protein [Medicago truncatula]
          Length = 575

 Score =  384 bits (986), Expect = e-104
 Identities = 185/249 (74%), Positives = 218/249 (87%), Gaps = 2/249 (0%)
 Frame = +3

Query: 6   IPCLYVFVDIKIDVNKLVGTIKLNLSDKDI--NKIVLAGTIQFGTAIRVAKPELEKLGFK 179
           IPCLYVFVDIKIDV+ LV T+KLN  D      +++LAGTIQF + IR  KPELEKLGF+
Sbjct: 142 IPCLYVFVDIKIDVDHLVDTVKLNSKDLGFMGKRVILAGTIQFASGIRAVKPELEKLGFR 201

Query: 180 VLVPQSKPLSAGEVLGCTAPTIMGKQFDEEGEIVLVFVADGRFHLEAFMIANPGIQAYRY 359
           VLVPQSKPLSAGE+LGCTAP + GK+ D++ E VLVFV+DGRFHLEAFMIANPG++A+RY
Sbjct: 202 VLVPQSKPLSAGEILGCTAPKV-GKELDDKEESVLVFVSDGRFHLEAFMIANPGVKAFRY 260

Query: 360 DPYIGKLFLEEYDHKGMKECRQNAIMKAREAKHWGVVLGTLGRQGNPRILDRLQKKMMEK 539
           DPY+GKLFLEEYDH GMK  R+NAI+KA+EA++WGVVLGTLGRQGNP+IL+RL+ KM +K
Sbjct: 261 DPYVGKLFLEEYDHVGMKGSRKNAILKAKEARNWGVVLGTLGRQGNPKILERLEMKMGDK 320

Query: 540 DLDWTVVLMSELSPARIALFGDSVDAWVQIACPRLSIDWGDAFVKPLLNPFEAEIALGDL 719
             D+TVVLMSELSP RI+LF DSVDAW+QIACPRLSIDWGDAFVKP+L PFEAEIALG +
Sbjct: 321 GFDYTVVLMSELSPYRISLFEDSVDAWIQIACPRLSIDWGDAFVKPVLTPFEAEIALGLI 380

Query: 720 PGWWERSGV 746
           PGWWE++ V
Sbjct: 381 PGWWEKTQV 389


>ref|XP_002529006.1| Diphthamide biosynthesis protein, putative [Ricinus communis]
           gi|223531546|gb|EEF33376.1| Diphthamide biosynthesis
           protein, putative [Ricinus communis]
          Length = 434

 Score =  376 bits (965), Expect = e-102
 Identities = 184/246 (74%), Positives = 207/246 (84%), Gaps = 1/246 (0%)
 Frame = +3

Query: 6   IPCLYVFVDIKIDVNKLVGTIKLNLSDKDINKIVLAGTIQFGTAIRVAKPELEKLGFKVL 185
           IPCLYVFV+IKIDV  L+ TIKLNL  KD   ++LAGTIQF +AIR AKPELE+LG   L
Sbjct: 135 IPCLYVFVEIKIDVECLISTIKLNL--KDYKSVILAGTIQFASAIREAKPELERLGLYAL 192

Query: 186 VPQSKPLSAGEVLGCTAPTIMGKQFDEEG-EIVLVFVADGRFHLEAFMIANPGIQAYRYD 362
           +PQSKPLSAGEVLGCTAP I        G E V+VFVADGRFHLEAFMIANPGI  +RYD
Sbjct: 193 IPQSKPLSAGEVLGCTAPKISSNSMVGGGSETVVVFVADGRFHLEAFMIANPGISTFRYD 252

Query: 363 PYIGKLFLEEYDHKGMKECRQNAIMKAREAKHWGVVLGTLGRQGNPRILDRLQKKMMEKD 542
           PY+GKLFLEEYDH+GMKE R+ AI  A+EA++WG+VLGTLGRQGNPRILDRL+KKM EK 
Sbjct: 253 PYMGKLFLEEYDHRGMKEVRKGAIQMAKEARNWGIVLGTLGRQGNPRILDRLEKKMREKG 312

Query: 543 LDWTVVLMSELSPARIALFGDSVDAWVQIACPRLSIDWGDAFVKPLLNPFEAEIALGDLP 722
             +TVVLMSE+SP RIALF D +DAW+QIACPRLSIDWG+AF KPLL PFEAEIALGDLP
Sbjct: 313 FSYTVVLMSEISPVRIALFEDCIDAWIQIACPRLSIDWGEAFKKPLLTPFEAEIALGDLP 372

Query: 723 GWWERS 740
           GWWE+S
Sbjct: 373 GWWEKS 378


>ref|XP_003540663.1| PREDICTED: diphthamide biosynthesis protein 1-like [Glycine max]
          Length = 459

 Score =  371 bits (952), Expect = e-100
 Identities = 181/251 (72%), Positives = 213/251 (84%), Gaps = 4/251 (1%)
 Frame = +3

Query: 6   IPCLYVFVDIKIDVNKLVGTIKLNLSDKDINKIVLAGTIQFGTAIRVAKPELEKLGFKVL 185
           IPCLYVFVDIKIDV   V T+ LNL  +    +V+AGTIQF +AIR AKP+LE+LGF+VL
Sbjct: 144 IPCLYVFVDIKIDVPHFVDTLSLNLLAQT-KTLVVAGTIQFASAIRAAKPQLEELGFRVL 202

Query: 186 VPQSKPLSAGEVLGCTAPTIMGKQF----DEEGEIVLVFVADGRFHLEAFMIANPGIQAY 353
           +PQSKPLSAGEVLGCTAP +   +      + GE VLVFVADGRFHLEAFMIANPGI+A+
Sbjct: 203 IPQSKPLSAGEVLGCTAPKVSSSKLLGGGGDIGECVLVFVADGRFHLEAFMIANPGIRAF 262

Query: 354 RYDPYIGKLFLEEYDHKGMKECRQNAIMKAREAKHWGVVLGTLGRQGNPRILDRLQKKMM 533
           RYDPY+GKLFLEEYDH GMK  R+NAI KAREA+ WG+VLGTLGRQGNPRIL+RL++ M 
Sbjct: 263 RYDPYMGKLFLEEYDHLGMKRSRKNAIFKAREARSWGLVLGTLGRQGNPRILERLERMMR 322

Query: 534 EKDLDWTVVLMSELSPARIALFGDSVDAWVQIACPRLSIDWGDAFVKPLLNPFEAEIALG 713
           E+ LD+TVVLMSE+SP RIALF DS+DAW+QIACPRLSIDWG+AFVKP+L PFEAE+ALG
Sbjct: 323 ERGLDYTVVLMSEMSPTRIALFEDSLDAWIQIACPRLSIDWGEAFVKPVLTPFEAEVALG 382

Query: 714 DLPGWWERSGV 746
            +PGWWE++ V
Sbjct: 383 VIPGWWEKNEV 393


>ref|XP_004136856.1| PREDICTED: diphthamide biosynthesis protein 1-like [Cucumis
           sativus] gi|449478949|ref|XP_004155462.1| PREDICTED:
           diphthamide biosynthesis protein 1-like [Cucumis
           sativus]
          Length = 462

 Score =  369 bits (948), Expect = e-100
 Identities = 174/245 (71%), Positives = 206/245 (84%)
 Frame = +3

Query: 6   IPCLYVFVDIKIDVNKLVGTIKLNLSDKDINKIVLAGTIQFGTAIRVAKPELEKLGFKVL 185
           IPCLYVFVDI IDV +LV T++LN+   D   +VLAGTIQF +AIR +K ELE+ G +VL
Sbjct: 149 IPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELERHGIRVL 208

Query: 186 VPQSKPLSAGEVLGCTAPTIMGKQFDEEGEIVLVFVADGRFHLEAFMIANPGIQAYRYDP 365
           +PQS+PLSAGEVLGCTAP +    F   GE V +FVADGRFHLEAFMIANPGI+ +RYDP
Sbjct: 209 IPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPGIRVFRYDP 268

Query: 366 YIGKLFLEEYDHKGMKECRQNAIMKAREAKHWGVVLGTLGRQGNPRILDRLQKKMMEKDL 545
           YIGKL LEEYDH+GMK  R+ AI+KA+EA +WG+VLGTLGRQGNP+IL+RL++KM  K  
Sbjct: 269 YIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKSKGF 328

Query: 546 DWTVVLMSELSPARIALFGDSVDAWVQIACPRLSIDWGDAFVKPLLNPFEAEIALGDLPG 725
           D+TVVLMSE+SP R+ALF DSVDAW+QIACPRLSIDWGDAF KPLL PFEAE+ALG +PG
Sbjct: 329 DYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGLIPG 388

Query: 726 WWERS 740
           WWER+
Sbjct: 389 WWERT 393