BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00028646
         (896 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P14912.1|4CL1_PETCR RecName: Full=4-coumarate--CoA ligase 1; ...   503   e-140
sp|P14913.1|4CL2_PETCR RecName: Full=4-coumarate--CoA ligase 1; ...   503   e-140
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   495   e-138
sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; ...   489   e-136
gb|ABD59789.2| 4-coumarate:CoA ligase [Arnebia euchroma]              489   e-136

>sp|P14912.1|4CL1_PETCR RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|20432|emb|CAA31696.1|
            unnamed protein product [Petroselinum crispum]
          Length = 544

 Score =  503 bits (1296), Expect = e-140
 Identities = 250/298 (83%), Positives = 270/298 (90%)
 Frame = -3

Query: 894  PLFHIYSLNSVLLCGLRAGAAIVIMHKFEIVPFLEVIQKYRVTIGPFVPPIVLAIAKSPV 715
            PLFHIYSLN+VL CGLRAG  I+IM KF+IVPFLE+IQKY+VTIGPFVPPIVLAIAKSPV
Sbjct: 235  PLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPV 294

Query: 714  VDDYDLSSVRTVMSGAAPLGKDLENAVRAKFPNAKLGQGYGMTEAGPVLAMCLGFAKQPF 535
            VD YDLSSVRTVMSGAAPLGK+LE+AVRAKFPNAKLGQGYGMTEAGPVLAMCL FAK+P+
Sbjct: 295  VDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPY 354

Query: 534  EIKSGACGTVVRNAEMKIIDPKTNISLSRNQRGEICIRGDQIMKGYLNDPDSTERTIDRD 355
            EIKSGACGTVVRNAEMKI+DP+TN SL RNQRGEICIRGDQIMKGYLNDP+ST  TID +
Sbjct: 355  EIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEE 414

Query: 354  GWLHTGDIGYIDEDDELFIVDRLKEIIKYKGFQVAPAELEAMLLTHPNISDAAVVPMIDE 175
            GWLHTGDIG+ID+DDELFIVDRLKEIIKYKGFQVAPAELEA+LLTHP ISDAAVVPMIDE
Sbjct: 415  GWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDE 474

Query: 174  KAGEVPVAFVARLNGCITTEDEVKQFISKQXXXXXXXXXXXXVDAIPKSPSGKILRKN 1
            KAGEVPVAFV R NG  TTE+E+KQF+SKQ            VDAIPKSPSGKILRK+
Sbjct: 475  KAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 532


>sp|P14913.1|4CL2_PETCR RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|20436|emb|CAA31697.1|
            unnamed protein product [Petroselinum crispum]
          Length = 544

 Score =  503 bits (1296), Expect = e-140
 Identities = 250/298 (83%), Positives = 270/298 (90%)
 Frame = -3

Query: 894  PLFHIYSLNSVLLCGLRAGAAIVIMHKFEIVPFLEVIQKYRVTIGPFVPPIVLAIAKSPV 715
            PLFHIYSLN+VL CGLRAG  I+IM KF+IVPFLE+IQKY+VTIGPFVPPIVLAIAKSPV
Sbjct: 235  PLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPV 294

Query: 714  VDDYDLSSVRTVMSGAAPLGKDLENAVRAKFPNAKLGQGYGMTEAGPVLAMCLGFAKQPF 535
            VD YDLSSVRTVMSGAAPLGK+LE+AVRAKFPNAKLGQGYGMTEAGPVLAMCL FAK+P+
Sbjct: 295  VDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPY 354

Query: 534  EIKSGACGTVVRNAEMKIIDPKTNISLSRNQRGEICIRGDQIMKGYLNDPDSTERTIDRD 355
            EIKSGACGTVVRNAEMKI+DP+TN SL RNQRGEICIRGDQIMKGYLNDP+ST  TID +
Sbjct: 355  EIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEE 414

Query: 354  GWLHTGDIGYIDEDDELFIVDRLKEIIKYKGFQVAPAELEAMLLTHPNISDAAVVPMIDE 175
            GWLHTGDIG+ID+DDELFIVDRLKEIIKYKGFQVAPAELEA+LLTHP ISDAAVVPMIDE
Sbjct: 415  GWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDE 474

Query: 174  KAGEVPVAFVARLNGCITTEDEVKQFISKQXXXXXXXXXXXXVDAIPKSPSGKILRKN 1
            KAGEVPVAFV R NG  TTE+E+KQF+SKQ            VDAIPKSPSGKILRK+
Sbjct: 475  KAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 532


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  495 bits (1275), Expect = e-138
 Identities = 248/298 (83%), Positives = 268/298 (89%)
 Frame = -3

Query: 894  PLFHIYSLNSVLLCGLRAGAAIVIMHKFEIVPFLEVIQKYRVTIGPFVPPIVLAIAKSPV 715
            PLFHIYSLNSVLLCGLR GAAI+IM KF+IV FLE+IQ+Y+VTIGPFVPPIVLAIAKSP+
Sbjct: 234  PLFHIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPM 293

Query: 714  VDDYDLSSVRTVMSGAAPLGKDLENAVRAKFPNAKLGQGYGMTEAGPVLAMCLGFAKQPF 535
            VDDYDLSSVRTVMSGAAPLGK+LE+ VRAKFPNAKLGQGYGMTEAGPVLAMCL FAK+PF
Sbjct: 294  VDDYDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 353

Query: 534  EIKSGACGTVVRNAEMKIIDPKTNISLSRNQRGEICIRGDQIMKGYLNDPDSTERTIDRD 355
            EIKSGACGTVVRNAEMKI+DPKT  SL RNQ GEICIRGDQIMKGYLNDP++T RTID++
Sbjct: 354  EIKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKE 413

Query: 354  GWLHTGDIGYIDEDDELFIVDRLKEIIKYKGFQVAPAELEAMLLTHPNISDAAVVPMIDE 175
            GWL+TGDIGYID+DDELFIVDRLKE+IKYKGFQVAPAELEA+LL HPNISDAAVVPM DE
Sbjct: 414  GWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDE 473

Query: 174  KAGEVPVAFVARLNGCITTEDEVKQFISKQXXXXXXXXXXXXVDAIPKSPSGKILRKN 1
            +AGEVPVAFV R NG   TEDEVK FISKQ            VDAIPKSPSGKILRK+
Sbjct: 474  QAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKD 531


>sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|1663722|gb|AAB18637.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 547

 Score =  489 bits (1259), Expect = e-136
 Identities = 241/298 (80%), Positives = 265/298 (88%)
 Frame = -3

Query: 894  PLFHIYSLNSVLLCGLRAGAAIVIMHKFEIVPFLEVIQKYRVTIGPFVPPIVLAIAKSPV 715
            PLFHIYSLNS+LLCGLR GAAI+IM KF+I PFLE+IQKY+V+IGPFVPPIVLAIAKSP+
Sbjct: 239  PLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPI 298

Query: 714  VDDYDLSSVRTVMSGAAPLGKDLENAVRAKFPNAKLGQGYGMTEAGPVLAMCLGFAKQPF 535
            VD YDLSSVRTVMSGAAPLGK+LE+AVR KFPNAKLGQGYGMTEAGPVLAMCL FAK+PF
Sbjct: 299  VDSYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 358

Query: 534  EIKSGACGTVVRNAEMKIIDPKTNISLSRNQRGEICIRGDQIMKGYLNDPDSTERTIDRD 355
            +IKSGACGTVVRNAEMKI+DP T  SL RNQ GEICIRGDQIMKGYLNDP++T RTID++
Sbjct: 359  DIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKE 418

Query: 354  GWLHTGDIGYIDEDDELFIVDRLKEIIKYKGFQVAPAELEAMLLTHPNISDAAVVPMIDE 175
            GWLHTGDIG+IDEDDELFIVDRLKE+IKYKGFQVAPAE+EA+LL HPNISDAAVVPM DE
Sbjct: 419  GWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDE 478

Query: 174  KAGEVPVAFVARLNGCITTEDEVKQFISKQXXXXXXXXXXXXVDAIPKSPSGKILRKN 1
            +AGEVPVAFV R NG   TEDEVK FISKQ            V+ +PKSPSGKILRK+
Sbjct: 479  QAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKD 536


>gb|ABD59789.2| 4-coumarate:CoA ligase [Arnebia euchroma]
          Length = 660

 Score =  489 bits (1259), Expect = e-136
 Identities = 241/298 (80%), Positives = 267/298 (89%)
 Frame = -3

Query: 894  PLFHIYSLNSVLLCGLRAGAAIVIMHKFEIVPFLEVIQKYRVTIGPFVPPIVLAIAKSPV 715
            PLFHIYS+NS+LLCGLR GAAI++MHKF+I PFLE+IQ+Y+VTIGPFVPPIVLAIAKS V
Sbjct: 237  PLFHIYSMNSILLCGLRVGAAILLMHKFDIAPFLELIQRYKVTIGPFVPPIVLAIAKSNV 296

Query: 714  VDDYDLSSVRTVMSGAAPLGKDLENAVRAKFPNAKLGQGYGMTEAGPVLAMCLGFAKQPF 535
            VD +DLSSVRTVMSGAAPLGK+LE+AVRAKFPNAKLGQGYGMTEAGPVLAMCL FAK+PF
Sbjct: 297  VDQFDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 356

Query: 534  EIKSGACGTVVRNAEMKIIDPKTNISLSRNQRGEICIRGDQIMKGYLNDPDSTERTIDRD 355
            EIKSGACGTVVRNAEMKIID +T +SL RNQ GEICIRGDQIMKGYLNDP++TERTID +
Sbjct: 357  EIKSGACGTVVRNAEMKIIDTETGVSLPRNQSGEICIRGDQIMKGYLNDPEATERTIDNE 416

Query: 354  GWLHTGDIGYIDEDDELFIVDRLKEIIKYKGFQVAPAELEAMLLTHPNISDAAVVPMIDE 175
            GWLHTGDIGYID+DDELFIVDRLKE+IKYKGFQVAPAELEA+L+THP +SDAAVV M DE
Sbjct: 417  GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPKVSDAAVVSMKDE 476

Query: 174  KAGEVPVAFVARLNGCITTEDEVKQFISKQXXXXXXXXXXXXVDAIPKSPSGKILRKN 1
             AGEVPVAFV R NG   TEDE+KQF+SKQ            VD+IPKSPSGKILRK+
Sbjct: 477  GAGEVPVAFVVRSNGSTITEDEIKQFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKD 534