BLASTX nr result
ID: Bupleurum21_contig00028454
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00028454
(1034 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002264085.1| PREDICTED: hyoscyamine 6-dioxygenase [Vitis ... 346 6e-93
ref|XP_003632389.1| PREDICTED: LOW QUALITY PROTEIN: hyoscyamine ... 342 9e-92
ref|XP_002330260.1| predicted protein [Populus trichocarpa] gi|2... 328 2e-87
ref|XP_002302051.1| predicted protein [Populus trichocarpa] gi|2... 323 6e-86
ref|XP_002302052.1| predicted protein [Populus trichocarpa] gi|2... 321 2e-85
>ref|XP_002264085.1| PREDICTED: hyoscyamine 6-dioxygenase [Vitis vinifera]
gi|297744130|emb|CBI37100.3| unnamed protein product
[Vitis vinifera]
Length = 341
Score = 346 bits (887), Expect = 6e-93
Identities = 165/322 (51%), Positives = 233/322 (72%), Gaps = 8/322 (2%)
Frame = -2
Query: 1003 MDMQSSSW-FNVQNVPQTYIFSEEDRPGDLIIPICDTIPVIDLGKPVST--AHKVQEIIQ 833
M+ SSW NVQ++P+ Y+ RPG LI+P C ++PV+DLGK S A +Q+I++
Sbjct: 1 MEKLVSSWSINVQSLPENYVLPPGQRPGKLIVPPCKSLPVVDLGKATSPDRAETIQKILE 60
Query: 832 ACREFRLFQVIDHGVPEDVISDTMSVFNDFFKLPEDDKTTIPN---EGGC-LYTGSSCFT 665
A EF FQVI+HGV +++I+++ S+F++FF +P +DK ++ + + C LYT + +
Sbjct: 61 ASWEFGFFQVINHGVSDNLINESRSIFSEFFNMPAEDKASLYSTDIDKSCILYTSNLNYD 120
Query: 664 KDGVHLWRDCLKHPCHPLEQCVHYWPEKPTNYRNVVTKYVGEIRKMSLKILELISKGLGL 485
+ VHLWRD L+HPCHP E+ V +WPEKPT YR +V + E+RK+SLKIL+LIS+GLGL
Sbjct: 121 IEEVHLWRDNLRHPCHPSEEYVQFWPEKPTKYREIVGAFSVEVRKLSLKILDLISEGLGL 180
Query: 484 EAEYLG-KLSEVQILTANNYPACPDPSLTLGILKHCDPSLITILYQGDIRGLQIVKDGRW 308
E Y G +LS+ Q+L+ N+YP CPDPSLTLG+ KH DP LI +L QGD+ GLQ+ KDG+W
Sbjct: 181 EQGYFGGELSKRQLLSVNHYPRCPDPSLTLGLPKHIDPCLIAVLLQGDVDGLQVYKDGQW 240
Query: 307 IAVEALPHAFVVILGNQLEIISNGKLRSTEHRAVTNPNEARKSIATLVNPSPNCIVEPAQ 128
+ VE LP+AFV+ +G+QL++ISNGKLR EHR VTNP EAR + T +NPSP+C+++PA+
Sbjct: 241 LGVEPLPYAFVINIGHQLQVISNGKLRGAEHRVVTNPREARTTPTTFINPSPDCVIQPAE 300
Query: 127 VLVDELNPSRYEPTLYRDFVHN 62
LV+ NP Y+ + DF N
Sbjct: 301 ALVNASNPPLYKAFKFIDFFKN 322
>ref|XP_003632389.1| PREDICTED: LOW QUALITY PROTEIN: hyoscyamine 6-dioxygenase-like [Vitis
vinifera]
Length = 339
Score = 342 bits (877), Expect = 9e-92
Identities = 168/320 (52%), Positives = 227/320 (70%), Gaps = 9/320 (2%)
Frame = -2
Query: 1003 MDMQSSSWF-NVQNVPQTYIFSEEDRPGDLIIPICDTIPVIDLGKPVST--AHKVQEIIQ 833
M+ SSW N Q++P+ Y+F RPG LI+P C +I +IDLGK S+ A +Q+I++
Sbjct: 1 MEALVSSWSTNAQSLPENYVFPPRXRPGKLIVPPCKSIALIDLGKAESSDRAETIQKILE 60
Query: 832 ACREFRLFQVIDHGVPEDVISDTMSVFNDFFKLPEDDKTTIP----NEGGCLYTGSSCFT 665
A +E+ LFQVI+HGV +++I D+MS+F +FF LP +DK ++ N+ LYT + +
Sbjct: 61 ASQEYGLFQVINHGVSDNLIDDSMSIFKEFFNLPAEDKASLYSTDLNKSCRLYTSNFTYE 120
Query: 664 KDGVHLWRDCLKHPCHPLEQCVHYWPEKPTNYRNVVTKYVGEIRKMSLKILELISKGLGL 485
+ VH W D L+HPCHPL+ V WPEKPT YR +V Y E+RK+SLKIL+LI +GLGL
Sbjct: 121 TEEVHFWSDILRHPCHPLQDQVQIWPEKPTRYREIVGAYSVELRKLSLKILDLICEGLGL 180
Query: 484 EAEYLGKLSEVQILTANNYPACPDPSLTLGILKHCDPSLITILYQGDIRGLQIVKDG--R 311
E G LS+ Q+L+ N+YP CPDPSLTLG+ KHCDP+L T++ QGD+ GLQ+ KDG +
Sbjct: 181 EQ---GXLSKGQMLSVNHYPRCPDPSLTLGLPKHCDPNLKTVVLQGDVGGLQVYKDGIGQ 237
Query: 310 WIAVEALPHAFVVILGNQLEIISNGKLRSTEHRAVTNPNEARKSIATLVNPSPNCIVEPA 131
W+ VE LPHAFVV +G QL+IISNGKLR EHR +TNP EAR + AT +NPSP+C++ PA
Sbjct: 238 WLGVEPLPHAFVVNIGYQLQIISNGKLRGAEHRVMTNPREARTTSATFINPSPDCVIHPA 297
Query: 130 QVLVDELNPSRYEPTLYRDF 71
+ LV+ NP Y+ Y DF
Sbjct: 298 EALVNSSNPPLYKAFKYVDF 317
>ref|XP_002330260.1| predicted protein [Populus trichocarpa] gi|222871716|gb|EEF08847.1|
predicted protein [Populus trichocarpa]
Length = 327
Score = 328 bits (840), Expect = 2e-87
Identities = 164/323 (50%), Positives = 228/323 (70%), Gaps = 11/323 (3%)
Frame = -2
Query: 1003 MDMQSSSWFNVQNVPQTYIFSEEDRPGDLIIPICDTIPVIDLGKPVSTAHK---VQEIIQ 833
M+ SSW NVQ VP+ YIF E RPG++ +IPVIDL + + +Q+I++
Sbjct: 1 MEKLVSSWNNVQTVPEKYIFPPEKRPGNVTFNASKSIPVIDLEAIAISQDREATIQKILK 60
Query: 832 ACREFRLFQVIDHGVPEDVISDTMSVFNDFFKLPEDDKTT------IPNEGGCLYTGSSC 671
A +++ FQVI+HGV ED+++DTMSVF +FF+LPE+DKT+ + N LYT S
Sbjct: 61 AGQDYGFFQVINHGVAEDLMNDTMSVFKEFFELPEEDKTSFYCEDAMRNNHCMLYTSSLL 120
Query: 670 FTKDGVHLWRDCLKHPCHPLEQCVHYWPEKPTNYRNVVTKYVGEIRKMSLKILELISKGL 491
+ + VHLWRD L++ CHPLE+C+ +WPEKPT YR+VV Y E++K++ ILEL+ +GL
Sbjct: 121 YATEDVHLWRDNLRNSCHPLEECIQHWPEKPTRYRHVVGAYATEVKKLAATILELLCEGL 180
Query: 490 GLEAEYLG-KLSEVQILTANNYPACPDPSLTLGILKHCDPSLITILYQ-GDIRGLQIVKD 317
GLE+ + G KLSE+ L N+YP CPDPSLTLG+ +HCDP+LITIL Q D+ GLQ++K+
Sbjct: 181 GLESGHFGGKLSEIPSLLVNHYPPCPDPSLTLGVSEHCDPNLITILQQDSDVFGLQVLKN 240
Query: 316 GRWIAVEALPHAFVVILGNQLEIISNGKLRSTEHRAVTNPNEARKSIATLVNPSPNCIVE 137
G WI VE + +AFVV +G Q++I+SN KLRS EHRAVTN +AR S+A P+ + IVE
Sbjct: 241 GEWIGVEPISNAFVVNMGYQMQIVSNNKLRSVEHRAVTNSEKARTSVAMFFIPNGDSIVE 300
Query: 136 PAQVLVDELNPSRYEPTLYRDFV 68
PA+ LVD NP+ Y+ Y++F+
Sbjct: 301 PAKALVDPRNPAIYKSFQYKEFM 323
>ref|XP_002302051.1| predicted protein [Populus trichocarpa] gi|222843777|gb|EEE81324.1|
predicted protein [Populus trichocarpa]
Length = 341
Score = 323 bits (827), Expect = 6e-86
Identities = 166/322 (51%), Positives = 221/322 (68%), Gaps = 8/322 (2%)
Frame = -2
Query: 1003 MDMQSSSWFNVQNVPQTYIFSEEDRPGDLIIPICDTIPVIDLGKPV--STAHKVQEIIQA 830
M+ SS N+Q++ + YIF E+ RPG + I +C++IPVIDLG + A+ QEI++A
Sbjct: 1 MEKLLSSRSNLQSLSEKYIFPEKIRPGKVAIALCESIPVIDLGDIAGQNRANIAQEILKA 60
Query: 829 CREFRLFQVIDHGVPEDVISDTMSVFNDFFKLPEDDKTTI----PNEGGCLYTGSSCFTK 662
+EF FQVI+HGV +++++DTMSVFN+ F++P +D I PN L+T S +
Sbjct: 61 SQEFGFFQVINHGVSKELMNDTMSVFNEVFEMPAEDLADIYSEDPNRSCRLFTSGSPYAN 120
Query: 661 DGVHLWRDCLKHPCHP-LEQCVHYWPEKPTNYRNVVTKYVGEIRKMSLKILELISKGLGL 485
+ VH WRD L+HPCHP L+ C+ WPEKPT YR VV Y E+ K++ ILELI++GLGL
Sbjct: 121 EDVHYWRDFLRHPCHPDLDACIQQWPEKPTRYRQVVGNYSTEVMKLASGILELITEGLGL 180
Query: 484 EAEYLG-KLSEVQILTANNYPACPDPSLTLGILKHCDPSLITILYQGDIRGLQIVKDGRW 308
E+ Y G KLSE L+ N YP CPDPSLTLG+ KHCDP+LITIL QGD+RGLQ+ KDG W
Sbjct: 181 ESGYFGGKLSENAFLSVNRYPPCPDPSLTLGLPKHCDPNLITILLQGDVRGLQVFKDGEW 240
Query: 307 IAVEALPHAFVVILGNQLEIISNGKLRSTEHRAVTNPNEARKSIATLVNPSPNCIVEPAQ 128
I V +P+AFV+ +G QL+IISN KL+ EHRAVTN +AR S A V+PS + IVEPA+
Sbjct: 241 IDVGPVPNAFVINIGYQLQIISNNKLKGAEHRAVTNSKDARTSAAFFVSPSRDSIVEPAR 300
Query: 127 VLVDELNPSRYEPTLYRDFVHN 62
L+ N Y + +F N
Sbjct: 301 ELIKAGNRPLYRAFEFTEFFSN 322
>ref|XP_002302052.1| predicted protein [Populus trichocarpa] gi|222843778|gb|EEE81325.1|
predicted protein [Populus trichocarpa]
Length = 341
Score = 321 bits (822), Expect = 2e-85
Identities = 165/322 (51%), Positives = 222/322 (68%), Gaps = 8/322 (2%)
Frame = -2
Query: 1003 MDMQSSSWFNVQNVPQTYIFSEEDRPGDLIIPICDTIPVIDLGKPV--STAHKVQEIIQA 830
M++ SS N+Q++ + YIF EE RPG + I +C++IPVIDLG + A+ QEI++A
Sbjct: 1 MEILVSSRCNLQSLSEKYIFPEEIRPGKVAIALCESIPVIDLGDIAGQNRANIAQEILKA 60
Query: 829 CREFRLFQVIDHGVPEDVISDTMSVFNDFFKLPEDDKTTI----PNEGGCLYTGSSCFTK 662
+EF FQVI+HGV +++++DTMSVF + F++P +D I P+ L+T S+ +
Sbjct: 61 SQEFGFFQVINHGVSKELMNDTMSVFKEVFEMPAEDLAGIYSEDPDRSCRLFTSSNSYAS 120
Query: 661 DGVHLWRDCLKHPCHP-LEQCVHYWPEKPTNYRNVVTKYVGEIRKMSLKILELISKGLGL 485
+ VH WRD L+HPCHP L+ C+ WPEKPT YR VV Y E+ K++ ILELI++GLGL
Sbjct: 121 EDVHNWRDFLRHPCHPDLDACIQQWPEKPTRYRQVVGNYSTEVMKLASGILELITEGLGL 180
Query: 484 EAEYLG-KLSEVQILTANNYPACPDPSLTLGILKHCDPSLITILYQGDIRGLQIVKDGRW 308
E+ Y G KLSE L+ N YP CPDPSLTLG+ KHCDP+LITIL QGD+ GLQ+ KDG W
Sbjct: 181 ESGYFGGKLSENAFLSVNRYPPCPDPSLTLGLPKHCDPNLITILLQGDVCGLQVFKDGEW 240
Query: 307 IAVEALPHAFVVILGNQLEIISNGKLRSTEHRAVTNPNEARKSIATLVNPSPNCIVEPAQ 128
I V +P+AFV+ +G QL+IISN KL+ EHRAVTN +AR S A V+PS + IVEPA+
Sbjct: 241 IGVGPVPNAFVINIGYQLQIISNNKLKGAEHRAVTNSKDARTSAAFFVSPSRDSIVEPAR 300
Query: 127 VLVDELNPSRYEPTLYRDFVHN 62
L+ E N Y + +F N
Sbjct: 301 ELIKEDNRPLYRAFEFTEFFSN 322